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1

Mesele, Tariku, Kumela Dibaba, and Esayas Mendesil. "Farmers’ Perceptions of Mexican Bean Weevil, Zabrotes subfasciatus (Boheman), and Pest Management Practices in Southern Ethiopia." Advances in Agriculture 2019 (April 11, 2019): 1–10. http://dx.doi.org/10.1155/2019/8193818.

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The common bean, Phaseolus vulgaris L., is one of the most important sources of protein in Ethiopia and other developing countries. However, the Mexican bean weevil, Zabrotes subfasciatus (Boheman), is a major constraint of stored common bean that causes qualitative and quantitative losses. This study was conducted to assess farmers’ knowledge and perceptions of Mexican bean weevil, to examine farmers’ pest management practices, and to identify challenges of pest management practices to develop integrated pest management (IPM) strategies. A survey of 148 smallholder common bean farmers was conducted at Mareka and Loma districts in southern Ethiopia. The majority (75%) of the farmers stored common bean in polypropylene bags while less than 10% of the farmers stored beans in ‘Diya’ (a traditional storage structure). Most (60.8%) farmers stored their beans in seed (threshed) form, and the majority (63.5%) of them stored their beans for 3-5 months. The majority of the farmers had knowledge about the Mexican bean weevil; they could identify damaged seeds based on the ‘holes’ on the seed (72.3%) and circular ‘windows’ on the seed (20.0%). About 45% of the farmers mentioned the high amount of loss at the time of storage. In addition, most farmers (53.4%) estimated 26-50% loss in storage. Most farmers reported the use of pesticidal plants for control of Mexican bean weevil, while only a few farmers reported they had applied insecticide in their store. Education level and family size had a positive and statistically significant impact on the use of pesticidal plants for the control of Mexican bean weevil. Furthermore, education level also influences the use of chemical insecticide. Results highlighted the need to use improved storage technology and to train farmers in postharvest handling practices as a component to develop IPM approach in order to minimize losses occurring along the value chains of the common bean.
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Cardona, Cesar, Carmen E. Posso, Julia Kornegay, Jose Valor, and Miguel Serrano. "Antibiosis Effects of Wild Dry Bean Accessions on the Mexican Bean Weevil and the Bean Weevil (Coleoptera: Bruchidae)." Journal of Economic Entomology 82, no. 1 (February 1, 1989): 310–15. http://dx.doi.org/10.1093/jee/82.1.310.

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3

Fory, L. F., F. Finardi-Filho, C. M. Quintero, T. C. Osborn, C. Cardona, M. J. Chrispeels, and J. E. Mayer. "α-Amylase Inhibitors in Resistance of Common Beans to the Mexican Bean Weevil and the Bean Weevil (Coleoptera: Bruchidae)." Journal of Economic Entomology 89, no. 1 (February 1, 1996): 204–10. http://dx.doi.org/10.1093/jee/89.1.204.

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4

Bliss, Fredrick A. "BREEDING STRATEGIES FOR DEVELOPING DRY BEAN CULTIVARS RESISTANT TO BRUCHID WEEVILS." HortScience 25, no. 9 (September 1990): 1123d—1123. http://dx.doi.org/10.21273/hortsci.25.9.1123d.

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The presence of arcelin protein in the seeds of common bean, Phaseolus vulgaris L., provides resistance to the Mexican bean weevil and to a lesser degree, the common bean weevil. Fast, accurate identification of single seeds containing arcelin facilitates the transfer of alleles for each of four different arcelin types through standard crossing procedures. Seed yields and other traits of near-isogenic lines that contain different alleles were comparable to the standard parent, Porrillo 70. Genotypic mixtures containing resistant and susceptible seeds produced seed yields comparable to Porrillo 70, which suggests that heterogeneous populations offer the potential for stable resistant cultivars.
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Mikami, Adriana Yatie, Aline Pissinati, Dáfila Fagotti, Ayres de Oliveira Menezes Júnior, and Maurício Ursi Ventura. "Control of the Mexican bean weevil Zabrotes subfasciatus with kaolin." Ciência Rural 40, no. 7 (July 23, 2010): 1497–501. http://dx.doi.org/10.1590/s0103-84782010005000108.

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The Mexican bean weevil Zabrotes subfasciatus (Coleoptera: Chrysomelidae: Bruchinae) is an important pest of stored beans in tropical regions. The efficiency of kaolin [with or without neem (Azadirachta indica) oil] and diatomaceous earth (DE) (standard treatment) was studied in laboratory aiming to obtain alternatives for chemical control of this insect. Insects were confined in plastic vials containing beans treated with kaolin (2, 4 and 8g kg-1), kaolin + neem [2g kg-1(5% neem oil)], diatomaceous earth (1g kg-1) and control. Mortality of adult insects, number of eggs and F1generation beetles emergency were assessed. Kaolin caused mortality of Z. subfasciatus, however higher periods and doses than DE were necessary to promote high mortality (100% or close). Kaolin treatments also affected female behavior because many eggs were placed in the vials walls. Number of emerged adults (F1) was similar between DE and kaolin; hence, kaolin constitutes a promising tool to the management of Z. subfasciatus. The mixture of kaolin and neem oil was not efficient in the control of Z. subfasciatus.
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Tigist, Shiferaw G., Rob Melis, Julia Sibiya, and Gemechu Keneni. "Evaluation of different Ethiopian common bean, Phaseolus vulgaris (Fabaceae) genotypes for host resistance to the Mexican bean weevil, Zabrotes subfasciatus (Coleoptera: Bruchidae)." International Journal of Tropical Insect Science 38, no. 01 (November 27, 2017): 1–15. http://dx.doi.org/10.1017/s1742758417000248.

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AbstractCommon bean (Phaseolus vulgarisL.) is amongst the most important grain legume crops in Africa in general, and Ethiopia in particular. The Mexican been weevil (Zabrotes subfasciatusBoheman) heavily attacks the grain of common bean. A total of 300 common bean entries were subjected to a ‘no-choice’ test at Melkassa Agricultural Research Centre, Ethiopia, using a randomized complete block design with three replications, to evaluate for resistance to the Mexican bean weevil. Data on insect and seed traits were collected and a significant level (P<0.01) of variation in all parameters measured was observed amongst genotypes. Relative resistance was recorded in landraces, improved genotypes and breeding lines, but the resistant genotypes, RAZ-11, RAZ-36, RAZ-2, RAZ-44, RAZ-120, RAZ-40 and MAZ-203, showed consistently complete resistance, with zero index of susceptibility value. Two other promising entries were also identified from the breeding lines (SCR-11) and landrace collections (NC-16) of Ethiopia. Stratified ranking diagrams showed that accessions from different eco-geographical origins in Ethiopia and those with different colours showed different patterns of response to infestation. The Ethiopian bean breeding programme should take up the resistant genotypes for a comprehensive yield trial at the national level and direct release them as commercial varieties. The incorporation of bean weevil resistance genes into adapted varieties through backcross breeding techniques, supported with marker assisted selection, seems to be the best strategy not only in terms of time saving but also in terms of effectiveness and efficiency.
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7

Pimenta, M., R. A. Mata, M. Venzon, D. N. C. Cunha, E. M. G. Fontes, C. S. S. Pires, and E. R. Sujii. "Survival and preference of cotton boll weevil adults for alternative food sources." Brazilian Journal of Biology 76, no. 2 (March 1, 2016): 387–95. http://dx.doi.org/10.1590/1519-6984.16214.

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Abstract Plants that have potential as alternative food source (floral nectar, pollen and plant tissues) to the boll weevil during the intercropping season were evaluated considering the prevalent conditions of Cerrado in the Central Brazil. Initially, we tested the nutritional adequacy for the survival of the insect of flower resource (pollen and nectar) provided by eight plant species (fennel, mexican sunflower, castor bean, okra, hibiscus, sorghum, pigeonpea and sunn hemp). Subsequently, we tested if the resources provided by the selected plants continued to be exploited by the boll weevil in the presence of cotton plant, its main food source average longevity of boll weevil adults was significantly longer when they were fed on hibiscus’ flowers (166.6 ± 74.4) and okra flowers (34.7 ± 28.9) than when they fed on flowers of other six species. Subsequently, the preference of the boll weevil in the use of resources was compared between okra or hibiscus and cotton plants, in dual choice experiments. Boll weevils preferred plants of the three species in the reproductive stages than those in vegetative stages. Although the cotton plant in the reproductive stage was the most preferred plant of all, boll weevils preferred flowering okra and hibiscus than cotton at the vegetative stage.
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8

Credland, P. F., and J. Dendy. "Intraspecific variation in bionomic characters of the Mexican bean weevil, Zabrotes subfasciatus." Entomologia Experimentalis et Applicata 65, no. 1 (October 1992): 39–47. http://dx.doi.org/10.1111/j.1570-7458.1992.tb01625.x.

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Bayih, Tegegne, Amanuel Tamiru, and Meseret Chimdessa Egigu. "Bioefficacy of Unitary and Binary Botanical Combinations Against Mexican Bean Weevil, Zabrotes subfasciatus (Coleoptera: Chrysomelidae)." International Journal of Tropical Insect Science 38, no. 03 (July 13, 2018): 205–15. http://dx.doi.org/10.1017/s1742758418000036.

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AbstractMexican bean weevil, Zabrotes subfasciatus Boheman, is a significant pest of stored beans (Phaseolus vulgaris L.) that causes considerable loss in the quality and quantity of bean grain in Africa. In the past two to three decades, researchers have attempted to identify botanicals with better insecticidal potential against storage pests. However, there is a lack of information on the potency of botanical combinations, their toxicology, and optimal application rates. This study was conducted to examine the bioefficacy of unitary and binary botanical combinations of Jatropha curcas (L.), Datura stramonium (L.), Chenopodium ambrosioides (L.), Schinus molle (L.), and Azadirachta indica (A. Juss) against Z. subfasciatus at 1% and 2% w/w dosages. The insecticidal activities of the botanicals were measured based on their effect on adult mortality of Z. subfasciatus and progeny reduction, grain weight loss, and weevil perforation index. Among botanical admixtures, binary combinations of C. ambrosioides + D. stramonium, J. curcas + C. ambrosioides, and S. molle + C. ambrosioides were the most potent. They resulted in the highest mortality of Z. subfasciatus, low weevil perforation index or none, and grain weight loss at the most moderate test dosage (1% w/w). Treatment with C. ambrosioides resulted in the highest efficacy when applied individually. We failed to observe any notable difference in toxicity between dosage levels among the binary botanical combinations. However, the effectiveness of unitary formulations improved with the increase in dosage rate. Application of botanicals did not adversely affect bean seed germination. Our study suggests that botanicals in binary mixtures may enhance the potency of constituent components for effective control of Z. subfasciatus.
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Lopes, Lucas Martins, Josiane Moura do Nascimento, Vanderley Borges Dos Santos, Lêda Rita Dantonino Faroni, and Adalberto Hipólito de Sousa. "EMERGENCE RATE OF THE MEXICAN BEAN WEEVIL IN VARIETIES OF BEANS FROM THE SOUTHWESTERN AMAZON." Revista Caatinga 31, no. 4 (December 2018): 1048–53. http://dx.doi.org/10.1590/1983-21252018v31n428rc.

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ABSTRACT Common beans (Phaseolus vulgaris L.) are one of the most important protein sources worldwide. However, infestation by bruchids compromises the storage of these beans after harvest. The objective of this study was to determine the emergence rate of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in four P. vulgaris varieties (Carioca Pitoco, Enxofre, Gorgutuba Vermelho, and Rosinha) cultivated in the southwestern Amazon (State of Acre, Brazil). The grains from each variety were infested with 50 non-sexed adult Z. subfasciatus, and the insects were collected 13 days after initiating the bioassays. The adult progeny was collected from each plant variety on alternate days from the beginning to the end of the emergence period, and they were counted (number of insects/jar). The sum of emerged insects per day (SEd) was determined from the beginning of the emergence period, from which the accumulated emergence was calculated (SEa= ΣSEd, % day). There were differences in the emergence rates of Z. subfasciatus among the bean varieties, with the rates being lower in the Gorgutuba Vermelho variety. Although this bean variety had the lowest peak of adult insect emergence, the period of insect development was not of increased length in this variety compared to the other varieties. In general, the Gorgutuba Vermelho variety appears to be resistant to Z. subfasciatus.
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11

Kornegay, Julia, Cesar Cardona, and Carmen E. Posso. "Inheritance of Resistance to Mexican Bean Weevil in Common Bean, Determined by Bioassay and Biochemical Tests." Crop Science 33, no. 3 (May 1993): 589–94. http://dx.doi.org/10.2135/cropsci1993.0011183x003300030034x.

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12

Ishimoto, M., and M. J. Chrispeels. "Protective Mechanism of the Mexican Bean Weevil against High Levels of [alpha]-Amylase Inhibitor in the Common Bean." Plant Physiology 111, no. 2 (June 1, 1996): 393–401. http://dx.doi.org/10.1104/pp.111.2.393.

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13

Cardona, Cesar, Julia Kornegay, Carmen E. Posso, Francisco Morales, and Hernando Ramirez. "Comparative value of four arcelin variants in the development of dry bean lines resistant to the Mexican bean weevil." Entomologia Experimentalis et Applicata 56, no. 2 (August 1990): 197–206. http://dx.doi.org/10.1111/j.1570-7458.1990.tb01397.x.

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14

SILVA, KAREN FERREIRA DA, EDSON LUIZ LOPES BALDIN, and LUIZ EDUARDO DA ROCHA PANNUTI. "USE OF BOTANICAL INSECTICIDES AS AN ALTERNATIVE FOR THE MANAGEMENT OF THE MEXICAN BEAN WEEVIL." Revista Caatinga 29, no. 2 (June 2016): 348–57. http://dx.doi.org/10.1590/1983-21252016v29n211rc.

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ABSTRACT: This study aimed to evaluate the insecticidal activity of eight botanical species in the behavior and biological development of Zabrotes subfasciatus (Coleoptera: Chrysomelidae: Bruchinae) under laboratory conditions. The botanical species were applied on bean grains (Phaseolus vulgaris Linnaeus) directly as powder or indirectly within TNT bags. Three laboratory assays were performed. First, a repellent activity test was performed by exposing twenty couples of Z. subfasciatus adults in a choice-test arena. Second, a mortality test was performed for seven days after infestation. Finally, the oviposition and emergency rates of adults (%) and the development from egg to adult (in days) were evaluated in seven couples (males and females) for seven days inside of a vial containing 0.3g of the powder from each botanical species and 10 g of bean grains (3% w.w-1). The study was conducted in a completely randomized design, and the treatments were arranged as a factorial design (2 x 9) with two factors (factor 1= powder and TNT bag application forms and factor 2= eight botanical species and control) with eight replications. The powder application form was more efficient in controlling Z. subfasciatus. Azadirachta indica (powder application), Ruta graveolens (powder application), and Piper aduncum (TNT bag) reduced the infestation of adults. The species A. inidica, Piper tuberculatum, Trichilia catigua, Pfaffia glomerata, R. graveolens, and Mentha pulegium inhibited the oviposition of the insects regardless of the formulation applied. R. graveolens (powder application) caused 100% of mortality. The powder application of R. graveolens and M. pulegium reduced egg viability and insect emergence; therefore, they are very promising alternatives to control Z. subfasciatus in stored grains.
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Bliss, F. A., J. C. Rosas, and P. A. A. Pereira. "DEPLOYMENT OF ARCELIN GENES FOR BRUCHID RESISTANCE IN COMMON BEANS." HortScience 27, no. 6 (June 1992): 597e—597. http://dx.doi.org/10.21273/hortsci.27.6.597e.

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The discovery of bruchid resistance in wild beans and the demonstration that theArcelin protein is responsible for the resistance, provide an opportunity to develop resistant cultivars of common bean, Phaseolus vulgaris L. Arcelin expression is controlled by multiple alleles, which impart different levels of insect resistance. In field tests in Honduras and Brazil, backcross-derived lines with the Arl-1 allele were most resistant, especially to Mexican bean weevil. Seed mixtures of 0.80 Arl-1:0.20 susceptible and equal amounts of Arl-1,Arl-2, and Arl-3, and Arl-4 containing seeds showed resistanc elevels and seed yields similar to lines homogeneous for Arl-1. Breeding lines uniform for appearance and agronomic performance, but heterogenous for resistance genes are being tested as potential new dry bean cultivars having stable insect resistance.
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de Sa, M. Fatima Grossi, T. Erik Mirkov, Masao Ishimoto, Gabriella Colucci, Kaye S. Bateman, and Maarten J. Chrispeels. "Molecular characterization of a bean α-amylase inhibitor that inhibits the α-amylase of the Mexican bean weevil Zabrotes subfasciatus." Planta 203, no. 3 (October 22, 1997): 295–303. http://dx.doi.org/10.1007/s004250050195.

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Silva, Cléia G. V., Hugo B. Zago, Hugo J. G. S. Júnior, Claudio A. G. da Camara, José Vargas de Oliveira, Reginaldo Barros, Manfred O. E. Schwartz, and Maria F. A. Lucena. "Composition and Insecticidal Activity of the Essential Oil ofCroton grewioidesBaill. against Mexican Bean Weevil (Zabrotes subfasciatusBoheman)." Journal of Essential Oil Research 20, no. 2 (March 2008): 179–82. http://dx.doi.org/10.1080/10412905.2008.9699985.

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SUZUKI, Kazunori, Masao ISHIMOTO, Fumio KIKUCHI, and Keisuke KITAMURA. "Growth Inhibitory Effect of an .ALPHA.-Amylase Inhibitor from the Wild Common Bean Resistant to the Mexican Bean Weevil (Zabrotes subfasciatus)." Ikushugaku zasshi 43, no. 2 (1993): 257–65. http://dx.doi.org/10.1270/jsbbs1951.43.257.

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19

Vilca Mallqui, K. S., J. L. Vieira, R. N. C. Guedes, and L. M. Gontijo. "Azadirachtin-Induced Hormesis Mediating Shift in Fecundity-Longevity Trade-Off in the Mexican Bean Weevil (Chrysomelidae: Bruchinae)." Journal of Economic Entomology 107, no. 2 (April 1, 2014): 860–66. http://dx.doi.org/10.1603/ec13526.

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., Ahsanul Haque, A. K. M. Faruquzzam ., Hasina Banu ., N. Islam ., Md Abdul Alim ., and Aleya Nasreen . "Pesticidal Efficacy of Some Indigenous Plant Oils Against the Mexican Bean Weevil, Zabrotes subfasciatus. Boheman. (Coleoptera: Bruchidae)." Journal of Biological Sciences 1, no. 11 (October 15, 2001): 1034–39. http://dx.doi.org/10.3923/jbs.2001.1034.1039.

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ISHIMOTO, Masao, and Keisuke KITAMURA. "Specific Inhibitory Acitivity and Inheritance of an .ALPHA.-Amylase Inhibitor in a Wild Common Bean Accession Resistant to the Mexican Bean Weevil." Ikushugaku zasshi 43, no. 1 (1993): 69–73. http://dx.doi.org/10.1270/jsbbs1951.43.69.

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Ortega, Jacobo Enrique Cruz, Leopoldo Partida Ruvalcaba, Teresa de Jesús Velásquez Alcaraz, Juan Eulogio Guerra Liera, Tomás Díaz Valdés, and Luz del Carmen Oliva Ortiz. "Effectiveness of Different Doses of Diatomaceous Earth on Mexican Bean Weevil (Zabrotes subfasciatus Boheman) in Culiacan, Sinaloa, Mexico." OALib 03, no. 12 (2016): 1–11. http://dx.doi.org/10.4236/oalib.1103228.

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Gonçalves, Gabriel Luiz Padoan, Simone Possedente De Lira, Danilo Soares Gissi, and José Djair Vendramim. "BIOACTIVITY OF EXTRACTS FROM SOLANACEAE AGAINST Zabrotes subfasciatus." Acta Biológica Colombiana 26, no. 1 (August 25, 2020): 62–71. http://dx.doi.org/10.15446/abc.v26n1.84712.

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The botanical family Solanaceae has many species producing compounds with insecticidal properties, e.g. nicotine and capsaicin, which are used for pest management in agriculture. This fact provides perspectives to identify insecticidal compounds in Brazilian native species of Solanaceae. In this study, we performed a screening with 25 ethanolic extracts from 17 Solanaceae species in order to evaluate their bioactivity against the Mexican bean weevil, Zabrotes subfasciatus(Coleoptera: Chrysomelidae: Bruchinae). The bioactivity of Solanaceae ethanolic extracts (2500 mg kg-1) was tested with residual contact bioassays. Adults ofZ. subfasciatus were exposed to treated bean grains, and adult mortality, oviposition, F1progeny and damages on grains were quantified. Most of the ethanolic extracts from Solanaceae reduced the number of eggs per sample, the egg-adult viability, the F1progeny and the damages on bean grains promoted byZ. subfasciatus, but none of them interfered on its sex ratio. Ethanolic extract from leaves of Solanum lycocarpumA. St.-Hil promoted the most promissory effects on Z. subfasciatus. This ethanolic extracts can be a suitable alternative to control Z. subfasciatus in stored beans, mainly for small farmers and organic farmers.
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Tigist, Shiferaw G., Rob Melis, Julia Sibiya, Beyene A. Amelework, Gemechu Keneni, and Asheber Tegene. "Genetic diversity analysis of common bean (Phaseolus vulgaris L.) genotypes for resistance to Mexican bean weevil (Zabrotes subfasciatus), using single nucleotide polymorphism and phenotypic markers." Acta Agriculturae Scandinavica, Section B — Soil & Plant Science 70, no. 6 (June 29, 2020): 495–506. http://dx.doi.org/10.1080/09064710.2020.1779339.

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Macedo, M. L. R., and D. C. S. Damico. "Effects of Protein Fractions from Zea Mays L. on Development and Survival of the Mexican Bean Weevil, Zabrotes subfasciatus (Boh.)." International Journal of Tropical Insect Science 20, no. 02 (June 2000): 135–39. http://dx.doi.org/10.1017/s1742758400018786.

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Barbosa, Flávia Rabelo, Massaru Yokoyama, Pedro Antônio Arraes Pereira, and Francisco José Pfeilsticker Zimmermann. "Effect of arcelin protein on the biology of Zabrotes subfasciatus (Boheman 1833), in dry beans." Pesquisa Agropecuária Brasileira 34, no. 10 (October 1999): 1805–10. http://dx.doi.org/10.1590/s0100-204x1999001000006.

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Arcelin is a seed protein found in wild beans (Phaseolus vulgaris) which gives resistance to Mexican bean weevil, Zabrotes subfasciatus (Boheman 1833) (Coleoptera: Bruchidae). Studies were carried out with the objective of estimating the effect of four alleles of protein arcelin (Arc1, Arc2, Arc3 and Arc4) on the biology of Z. subfasciatus. The experiment was carried out in laboratory at Embrapa-Centro Nacional de Pesquisa de Arroz e Feijão, in Santo Antônio de Goiás, GO, Brazil, under non controlled conditions. The highest levels of antibiosis to Z. subfasciatus were observed in Arc1, with reduction in the number of eggs, number of emerged adults, adults longevity. In the line Arc2 only reduction in the number of emerged adults was observed. The lines Arc3 and Arc4 showed low efficiency on the reduction of progeny of Z. subfasciatus and effects in the longevity and egg-adult cycle were not detected. Insect sexual ratio was not altered by the presence of Arc1, Arc2, Arc3 and Arc4 in the seeds.
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Dively, Galen P., Terrence Patton, Lindsay Barranco, and Kelly Kulhanek. "Comparative Efficacy of Common Active Ingredients in Organic Insecticides Against Difficult to Control Insect Pests." Insects 11, no. 9 (September 8, 2020): 614. http://dx.doi.org/10.3390/insects11090614.

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There exists a lack of control efficacy information to enable decision-making about which organic insecticide product works best for a given insect pest. Here, we summarize results of 153 field trials on the control efficacy of common active ingredients in organic insecticides against 12 groups of the most difficult to control insect pests. These trials evaluated primarily the organic products Entrust (spinosad), Azera (pyrethrin and azadirachtin), PyGanic (pyrethrin) and Neemix (azadirachtin), which reduced pest infestations by an overall 73.9%, 61.7%, 48.6% and 46.1% respectively, averaged across all trials. Entrust was the most effective control option for many insect pests, particularly providing >75% control of flea beetles, Colorado potato beetle, cabbageworms and alfalfa weevil, but was relatively ineffective against true bugs and aphids. Azera provided >75% control of green peach aphid, flea beetles, Japanese beetle, Mexican bean beetle, potato leafhopper and cabbageworms. PyGanic was less effective than Entrust and Azera but still provided >75% control of green peach aphid, flea beetles and potato leafhopper. The growth inhibition effects of azadirachtin in Neemix were particularly effective against larvae of Mexican bean beetle and Colorado potato beetle but was generally less effective in trials with insect infestations consisting mainly of adult stages. Those insect pests that were particularly difficult to control included thrips, stinkbugs, cucumber beetles and fruitworms. Several caveats pertaining to the application of the results are discussed.
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Zambre, M., A. Goossens, C. Cardona, M. Van Montagu, N. Terryn, and G. Angenon. "A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil." Theoretical and Applied Genetics 110, no. 5 (February 9, 2005): 914–24. http://dx.doi.org/10.1007/s00122-004-1910-7.

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Lagarda-Diaz, Irlanda, Miguel Ángel Hernández-Oñate, José Ángel Huerta-Ocampo, Ana M. Guzmán-Partida, Joy Winzerling, Dawn Geiser, and Luz Vázquez-Moreno. "Gene Sequences of Potential Targets of Insecticidal PF2 Lectin Identified from the Larval De Novo Transcriptome of the Mexican Bean Weevil (Zabrotes Subfasciatus; Boheman 1833)." Insects 11, no. 11 (October 27, 2020): 736. http://dx.doi.org/10.3390/insects11110736.

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The available genomic and proteomic information of non-model organisms is often underrepresented in public databases hindering their study at molecular, cellular, and physiological levels. Information on Zabrotes subfasciatus (Mexican bean weevil) is poorly represented in databases, yet it is a major pest of common beans. We report the transcriptome of Z. subfasciatus larvae; transcripts were sequenced using an Illumina RNA-Seq technology and assembled de novo identifying 29,029 unigenes with an average size of 1168 bp and an N50 value of 2196 bp. About 15,124 unigenes (52%) were functionally annotated and categorized. Further analysis revealed 30 unigene sequences encoding putative targets of the insecticidal PF2 lectin. The complete deduced amino acid sequences of eight selected proteins potentially related to insecticidal mechanism of Palo Fierro 2 (PF2) were used for predicting probable N-glycosylation sites and analyzing phylogenetic relationships with insect sequences. This work provides a dramatic increase in the genetic resources available for Coleopterans and set the basis for developing future studies on biological aspects and potential control strategies for Z. subfasciatus.
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Meireles, Elaine A., Cíntia N. B. Carneiro, Renato A. DaMatta, Richard I. Samuels, and Carlos P. Silva. "Digestion of Starch Granules from Maize, Potato and Wheat by Larvae of the the Yellow Mealworm,Tenebrio molitorand the Mexican Bean Weevil,Zabrotes subfasciatus." Journal of Insect Science 9, no. 43 (June 2009): 1–8. http://dx.doi.org/10.1673/031.009.4301.

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Credland, Peter F., and Julie Dendy. "Comparison of seed consumption and the practical use of insect weight in determining effects of host seed on the Mexican bean weevil, Zabrotes subfasciatus (Boh.)." Journal of Stored Products Research 28, no. 4 (October 1992): 225–34. http://dx.doi.org/10.1016/0022-474x(92)90001-7.

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Bifano, Thaís D., Richard I. Samuels, Daniel Alexandre, and Carlos P. Silva. "Host-mediated induction of α-amylases by larvae of the Mexican bean weevil Zabrotes subfasciatus (Coleoptera: Chrysomelidae: Bruchinae) is irreversible and observed from the initiation of the feeding period." Archives of Insect Biochemistry and Physiology 74, no. 4 (July 13, 2010): 247–60. http://dx.doi.org/10.1002/arch.20375.

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Weaver, David K., Carl D. Wells, Florence V. Dunkel, Wolfgang Bertsch, Sharlene E. Sing, and Shobha Sriharan. "Insecticidal Activity of Floral, Foliar, and Root Extracts of Tagetes minuta (Asterales: Asteraceae) Against Adult Mexican Bean Weevils (Coleoptera: Bruchidae)." Journal of Economic Entomology 87, no. 6 (December 1, 1994): 1718–25. http://dx.doi.org/10.1093/jee/87.6.1718.

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Mushobozy, Deus, Gerod Nganilevanu, Sosthenus Ruheza, and George Swella. "Plant Oils as Common Bean (Phaseolus Vulgaris L.) Seed Protectants Against Infestations by the Mexican Bean Weevil Zabrotes Subfasciatus (Boh.)." Journal of Plant Protection Research 49, no. 1 (January 1, 2009). http://dx.doi.org/10.2478/v10045-009-0005-5.

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35

Chamorro, Maria Lourdes, and Maxwell Barclay. "On the identity of a U.S. intercepted Conotrachelus Dejean (Coleoptera: Curculionidae) with avocado (Persea americana)." Biodiversity Data Journal 6 (November 2, 2018). http://dx.doi.org/10.3897/bdj.6.e26362.

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The multimillion-dollar avocado industry is threatened by a number of serious insect pests, including at least seven species of Curculionidae. Of these, three Conotrachelus species are known to develop and feed on avocados: Conotrachelus aguacatae Barber, Conotrachelus perseae Barber and C. serpentinus (Klug); the first two are of economic importance. Recently, a series of unrecognised Conotrachelus was intercepted with avocado and other commodities by the USDA at various southern U.S. ports of entry. The species most closely resembled the U.S. native Conotrachelus posticatus Boheman. Given the threat posed by certain species of Conotrachelus to avocado, the identity and biology of intercepted unknown Conotrachelus species becomes a matter of much concern for regulators due to the potential risk posed by non-native species to local agriculture. This study aims to determine the identity, which in turn may shed light on the biology and native distribution, of possible new non-U.S.-native weevils and provide the tools necessary to distinguish amongst phenotypically similar native species. Amongst the unknown Conotrachelus weevils intercepted with avocados at certain U.S. ports of entry is Conotrachelus lobatus Champion. This poorly known species resembles a commonly collected, phenotypically variable indigenous U.S. species, Conotrachelus posticatus, which, on occasion, is also intercepted with avocado. Conotrachelus lobatus has been collected, since the early 1900s until today, along a narrow corridor in the southwest Mexican states of Michoacan, Jalisco and Nayarit. Specimen label data in natural history collections suggests the presence of this species in large numbers in early July in the avocado growing region of Mexico and, based on notes from former curators, appears to breed in acorns of the Mexican endemic oak species Quercus obtusata. The interception of C. posticatus and C. lobatus wth avocado does not imply strict biological association, however it reveals an important pattern of a non-native species' potential for introduction and its potential vector. Understanding all aspects of an organism's biology will better equip growers, as well as regulators, with effective and well-informed management strategies. Characters are imaged and discussed in order to help distinguish some Conotrachelus species belonging to Conotrachelus group II designated by Schoof (1942). Some characters of particular importance are the shape of the metauncus; shape of the lateral margin of the elytra and presence/absence of costate first and second elytral intervals. This study includes high-resolution images of seven Conotrachelus species, including the known avocado pests C. aguacatae and C. perseae, as well as the first images of C. lobatus, C. scoparius Champion and C. squamifrons Champion. The latter three species are not USA natives and were not included in Schoof's work. This study also confirms the important role played by natural history collections in anchoring the species' name through the study of types, which allows for the linking of biological and distribution data over time. Lectotypes are herein designated for C. lobatus and C. squamifrons.
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