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Journal articles on the topic 'MHA (Mueller Hinton Agar)'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Marliana, Nina, Iis Kurniati, Cecep Patria, Asep Dermawan, and Yuliansyah Sundara Mulia. "UJI KEPEKAAN ANTIBIOTIKA Staphylococcus aureus DAN Escherichia coli PADA MEDIA TAHU PENGGANTI MUELLER HINTON AGAR." JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG 14, no. 2 (2022): 319–24. http://dx.doi.org/10.34011/juriskesbdg.v14i2.2033.

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Mueller Hinton Agar merupakan media yang direkomendasikan oleh World Health Organization (WHO) untuk uji kepekaan bakteri terhadap antibiotika sedangkan Media Tahu secara umum dapat digunakan sebagai basal seperti halnya agar nutrient untuk menyiapkan media kaya dengan penambahan suplemen tertentu misalnya darah. Keduanya merupakan media padat, non selektif dan mempunyai kemiripan susunan makanan. Penelitian ini bertujuan menentukan perbedaan hasil uji kepekaan Staphylococcus aureus dan Escherichia coli menggunakan Mueller Hinton Agar dan Media Tahu. Penelitian uji kepekaan menggunakan Mueller

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Otajevwo Dafinone Festus and Osawaru Osama Emmanuella. "Testing the efficacy of Mueller Hinton agar over Nutrient agar for optimal antibiotic sensitivity testing response by selected clinical bacterial pathogens." GSC Advanced Research and Reviews 5, no. 2 (2020): 061–74. http://dx.doi.org/10.30574/gscarr.2020.5.2.0037.

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The efficacy of Mueller Hinton agar over Nutrient agar in terms of antibiotic sensitivity testing for optimal antibiotic response by selected clinical bacterial pathogens was carried out in this study. Clinical bacterial pathogens used for the study were Pseudomonas aerµginosa, Enterococcus spp, Escherichia coli and Klebsiella pneumoniae. Standard and locally manufactured antibiotic discs used were by Abtek Biologicals Ltd, Liverpool and Maxicare Medical Laboratory, Nigeria respectively. Antibiotic sensitivity testing (AST) was by agar diffusion method. Pure cultures of each isolate were subcu

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Otajevwo, Dafinone Festus, and Osama Emmanuella Osawaru. "Testing the efficacy of Mueller Hinton agar over Nutrient agar for optimal antibiotic sensitivity testing response by selected clinical bacterial pathogens." GSC Advanced Research and Reviews 5, no. 2 (2020): 061–74. https://doi.org/10.5281/zenodo.4319567.

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The efficacy of Mueller Hinton agar over Nutrient agar in terms of antibiotic sensitivity testing for optimal antibiotic response by selected clinical bacterial pathogens was carried out in this study. Clinical bacterial pathogens used for the study were <em>Pseudomonas aerµginosa, Enterococcus</em> spp, <em>Escherichia coli</em> and <em>Klebsiella pneumoniae.</em> Standard and locally manufactured antibiotic discs used were by Abtek Biologicals Ltd, Liverpool and Maxicare Medical Laboratory, Nigeria respectively. Antibiotic sensitivity testing (AST) was by

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Daniela, V. Pencheva, S. Ivancheva Katia, V.Rumenkina Mina, M. Al-Dzhasem Aleksandrina, and N. Ivanov Ivan. "In Search of the Truth about the Quality of Mueller Hinton Agar and Tested Antimicrobial Discs." Pharmaceutical and Chemical Journal 5, no. 1 (2018): 145–52. https://doi.org/10.5281/zenodo.13893762.

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<em>Objectives</em>: The main goal of the provided experiments was to test the influence of the used in the Disc Diffusion Method (DDM) Mueller Hinton agar (MHA) and to establish its reflection on the interpretation of the antimicrobial test. <em>Methods: </em>This study is based on developed microbiological methodology and outworked protocol for qualification of the Mueller Hinton agar when it is used in Disc Diffusion Method in order to obtain reliable and reproducible results on quality indicators for MHA, appointed by the Clinical and Laboratory Standards Institute (CLSI) and European Comm

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Kusmayadi, Andri, Lovita Adriani, Abun, Muchtaridi, and Ujang Hidayat Tanuwiria. "Effects of Mangosteen Peel Extract Encapsulated By Maltodextrin On Antibacterial Activities of Escherichia coli." Jurnal Biologi Tropis 23, no. 1 (2023): 164–68. http://dx.doi.org/10.29303/jbt.v23i1.4627.

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Mangosteen peel extract contains good antibacterial compounds and is thought to be better when microencapsulated is formed. This study aimed to determine the effects of formulation of mangosteen peel ethanol extract associated to maltodextrin on activities of Escherichia coli. It was conducted by disk diffusion method according to Kirby-Bauer test in Mueller Hinton Agar (MHA) using three different concentrations namely: 100, 75, and 50 (%). Each formulation was tested as many as 10 µL disk -1 consisted of each treatment. The employed maltodextrin was obtained by enzymatic processing of arenga

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Chelaru, Edgar-Costin, Andrei-Alexandru Muntean, Mădălina-Maria Muntean та ін. "Oxacillin-Supplemented Mueller-Hinton Agar for In Vitro Inhibition of Ambler Class C β-Lactamases in Enterobacterales". Antibiotics 14, № 6 (2025): 616. https://doi.org/10.3390/antibiotics14060616.

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Background: The increasing incidence of infection with Gram-negative bacilli (GNB) producing broad-spectrum β-lactamases, such as extended-spectrum β-lactamases (ESBLs), cephalosporinases (AmpCs), and carbapenemases, has become a great clinical concern. AmpCs are found in many clinically relevant Enterobacterales, where they may compromise the effectiveness of most β-lactams, including carbapenems when associated with an impaired outer membrane. Detection and distinction between these resistance mechanisms are crucial for antimicrobial therapy and for implementation of proper infection control

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7

Wilson, Wildiani, and Dewi Yuniliani. "Potensi Antibakteri Isolat Bakteri Endofit dari Tanaman Purwoceng (Pimpinella pruatjan Molk.) Terhadap Klebsiella pneumoniae dan Salmonella typhi." Biomedika 12, no. 1 (2019): 74–78. http://dx.doi.org/10.31001/biomedika.v12i1.430.

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Penelitian ini bertujuan untuk mengetahui isolat bakteri endofit purwoceng sebagai antibakteri. Pengujian antibakteri dilakukan dengan menggunakan metode difusi sumuran. Isolat bakteri endofit potensial terlebih dahulu diinokulasi ke dalam media fermentasi cair selama 52 jam dan selanjutnya dilakukan proses ekstraksi menggunakan pelarut n-Heksan. Ekstrak kasar ini digunakan untuk pengujian antibakteri terhadap K. pneumoniae dan S. typhi. Aktivitas antibakteri isolat bakteri endofit menggunakan media MHA (Mueller Hinton Agar) dengan ketebalan 6 mm dan ukuran diameter sumuran 5 mm. Hasil penelit

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Miller, Shelley A., James Karichu, Peggy Kohner, et al. "Multicenter Evaluation of a Modified Cefoxitin Disk Diffusion Method and PBP2a Testing To PredictmecA-Mediated Oxacillin Resistance in Atypical Staphylococcus aureus." Journal of Clinical Microbiology 55, no. 2 (2016): 485–94. http://dx.doi.org/10.1128/jcm.02211-16.

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ABSTRACTPhenotypic variants ofStaphylococcus aureusthat display small colonies, reduced pigmentation, and decreased hemolysis and/or coagulase activity are periodically isolated by the clinical laboratory. Antimicrobial susceptibility testing (AST) of these isolates is complicated, because many do not grow on routine AST media, including Mueller-Hinton agar (MHA) and cation-adjusted Mueller-Hinton broth. This multicenter study evaluated cefoxitin disk diffusion for 37 atypicalS. aureusisolates (156 readings) with MHA supplemented with 5% sheep's blood (BMHA), usingmecAPCR as the reference stan

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Taiwo, Samuel Sunday, Titilope Bosede Bamigboye, Osatohanmwen Odaro, Olusegun Adelowo Adefioye, and Solomon Olufemi Fadiora. "Vancomycin intermediate and high level vancomycin resistant Staphylococcus aureus clinical isolates in Osogbo, Nigeria." Microbiology Research 2, no. 1 (2011): 6. http://dx.doi.org/10.4081/mr.2011.e6.

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<p>The decreased vancomycin susceptibility and subsequent emergence of vancomycin resistant <em>Staphylococcus aureus </em>(VRSA) strains already multi-resistant to antibiotics is a major public health problem. In 2009, the Clinical and Laboratory Standards Institute (CLSI) altered its guidelines for vancomycin susceptibility testing in <em>S. aureus</em> and recent data suggests the possibility that VRSA may emerge more frequently than previously expected. Against this background, we conducted a study to ascertain the susceptibility status of clinical <em>S

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TSAI, CHIN-EN, and FUSAO KONDO. "Improved Agar Diffusion Method for Detecting Residual Antimicrobial Agents." Journal of Food Protection 64, no. 3 (2001): 361–66. http://dx.doi.org/10.4315/0362-028x-64.3.361.

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The improved agar diffusion method for determination of residual antimicrobial agents was investigated, and the sensitivities of various combinations of test organisms and assay media were determined using 7 organisms, 5 media, and 31 antimicrobial agents. Bacillus stearothermophilus and synthetic assay medium (SAM) showed the greatest sensitivity for screening penicillins (penicillin G and ampicillin). The combination of Bacillus subtilis and minimum medium (MM) was the most sensitive for tetracyclines (oxytetracycline and chlortetracycline), B. stearothermophilus and SAM or Micrococcus luteu

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Priyono, Sumarnie, and Praptiwi Praptiwi. "IDENTIFIKASI SENYAWA KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK Piper Sp. ASAL PAPUA." Jurnal Teknologi Lingkungan 10, no. 3 (2016): 271. http://dx.doi.org/10.29122/jtl.v10i3.1473.

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The aim of this study was to identity the chemical compounds of the piper sp.Fruit originated from Papua, that has been used as “sirih pinang”(chew betle)and to determine the antibacterial activity of hexane and chloroform extraxt ofpiper sp. to Gram + bacteria isolates(Bacillus subtilis, Listeria monocytogenesdan Staphylococcus aureus) and Gram – bacteria isolates(Salmonellatyphimurium, Eschericia coli and Pseudomonas pseudomallei). The extractconcentration were 50 % and 25 %, and every treatment consisted of 3replications. The bacteriae growth media was Mueller Hinton Agar(MHA). Theresult of

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Amalia, Martina, Irma Ervina, Silvia, and Erdi E. Nasution. "Antimicrobial activity of chitosan nanoparticles loaded with 0.7% tetracycline against porphyromonas gingivalis." Journal of Dentomaxillofacial Science 8, no. 3 (2023): 169–72. http://dx.doi.org/10.15562/jdmfs.v8i3.1652.

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Objectives: Nanoparticle technology has also come to the fore as a viable drug delivery strategy, providing opportunities for controlled release, protecting the active ingredient from enzymatic or environmental degradation, and local retention. Porphyromonas gingivalis is a Gram-negative obligate anaerobe rod involved in the etiology of periodontal disease. Tetracycline has been widely used as an adjuvant in periodontal therapy due to its antibacterial efficacy of these drugs.  Material and Methods: Samples of this study were the pure culture of P. gingivalis (ATCC 33 277) in Mueller Hin

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Menezes, Ralciane de Paula, Mário Paulo Amante Penatti, and Reginaldo dos Santos Pedroso. "Different culture media containing methyldopa for melanin production by Cryptococcus species." Revista da Sociedade Brasileira de Medicina Tropical 44, no. 5 (2011): 591–94. http://dx.doi.org/10.1590/s0037-86822011000500012.

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INTRODUCTION: Melanin production by species of Cryptococcus is widely used to characterize C. neoformans complex in mycology laboratories. This study aims to test the efficacy of methyldopa from pharmaceutical tablet as a substrate for melanin production, to compare the production of melanin using different agar base added with methyldopa, and to compare the melanin produced in those media with that produced in Niger seed agar and sunflower seed agar by C. neoformans, C. laurentii, and C. albidus. Two isolates of each species, C. neoformans, C. laurentii, and C. albidus, and one of Candida alb

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14

Mohammed, Sudad Jasim, Adil Turki Al-Musawi, Aliaa Saadoon Al-Fraji, and Hayder Shannoon Kareem. "Comparison of three culture media in assessing the sensitivity of antibiotics to common foodborne microorganisms." Journal of Medicine and Life 15, no. 5 (2022): 645–49. http://dx.doi.org/10.25122/jml-2021-0404.

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This study aimed to determine the antibiotic susceptibility of seven antibiotics, (Amoxicillin (AX), Ampicillin (AM), Chloramphenicol (C), Ciprofloxacin (CIP), Doxycycline (DO), Gentamicin (CN) and Neomycin (N)) on some common microorganisms that cause food poisoning. Furthermore, we aimed to compare three types of culture media in assessing antibiotics susceptibility. A sensitivity test was carried out using six bacterial isolates: Micrococcus spp., Staphylococcus aureus, Escherichia coli, Salmonella spp., Proteus mirabilis, Pseudomonas aeruginosa. These bacterial isolates were identified at

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Kosilova, I. S., L. V. Domotenko, N. K. Fursova, S. V. Dentovskaya, M. G. Ershova, and A. P. Shepelin. "TRIALS OF THE DOMESTICALLY PRODUCED NUTRIENT MEDIUM «AGAR MULLER-HINTON II - OBOLENSK»." Russian Clinical Laboratory Diagnostics 64, no. 6 (2019): 360–67. http://dx.doi.org/10.18821/0869-2084-2019-64-6-360-367.

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The results of the comparative tests of the «Agar Muller-Hinton II - Obolensk» nutrient medium developed in SRCAMB, Obolensk, and the control nutrient medium imported «Mueller Hinton II Agar» are presented in the study. The susceptibility of bacterial clinical strains to antimicrobial agents (AMP) was determined by the disc diffusion method and the method of gradient diffusion (E-test). The carbapenemase activity of the strains carrying the carbapenemase genes was determined by CIM-test. Total 173 characterized bacterial strains of species Klebsiella pneumoniae, Pseudomonas aeruginosa, Acineto

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Pfaller, M. A., S. A. Messer, Å. Karlsson, and A. Bolmström. "Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media." Journal of Clinical Microbiology 36, no. 9 (1998): 2586–89. http://dx.doi.org/10.1128/jcm.36.9.2586-2589.1998.

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The performance of the Etest for fluconazole susceptibility testing of 402 yeast isolates was assessed against the National Committee for Clinical Laboratory Standards (NCCLS) microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48 h at 35°C. Etest MICs were determined with RPMI agar containing 2% glucose (RPG), Casitone agar (CAS), and Mueller-Hinton agar (MHA) and were read after incubation for 48 h at 35°C. The yeast isolates included Candida albicans(n = 161), Candida glabrata(n = 41), Candida tropicalis(n = 35), Candida para

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Abdullah, Abdullah. "DETEKSI KEBERADAAN BAKTERI RESISTEN LOGAM MERKURI (Hg) PADA PENAMBANGAN EMAS TANPA IZIN (PETI) DI SIMPI, SEKADAU, KALIMANTAN BARAT." Indonesian Journal of Pure and Applied Chemistry 1, no. 2 (2018): 56. http://dx.doi.org/10.26418/indonesian.v1i2.30529.

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The purpose of this research is to know the existence of mercury metal degradation bacteria in the activity of illegal gold mining (PETI) in Simpi Village, Belitang Hilir sub-district, Sekadau District, West Kalimantan. The samples were taken from one location with three different sampling points. Isolation of bacteria by pour plate method in NA-HgCl medium. Detection of bacteria by paper disc method based on inhibit zone resistant of Mueller-Hinton Agar (MHA) with mercury levels such as 10 mg/L, 20 mg/L and 30 mg/L . The results showed that it was found that one pure isolate (PP) showed the m

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Amalda, Elfahra Casanza, Mifta Rizqina Amalia, Ainrisq Auliya Rifai, Yohana Maria Karo, Lady Konfidenia Chintari, and Habyb Palyoga. "Isolation, Identification, and Antibiotic Susceptibility Test of Streptococcus spp. from a Cat Suspected of Respiratory Tract Disease at Nong Lam University, Vietnam." Veterinary Biomedical and Clinical Journal 6, no. 2 (2024): 47–53. https://doi.org/10.21776/ub.vetbioclinj.2024.006.02.1.

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The nasal sample of a suspected respiratory tract disease cat, which is characterized by having a yellowish-colored nasal discharge, was obtained from a clinic in Ho Chi Minh City to be brought to the laboratory using the Stuart Amies transport medium. Samples were isolated on blood agar plate (BAP) media anaerobically. Gram staining and catalase tests were performed to identify the bacterial species. Furthermore, we conducted antibiotic sensitivity tests using Mueller-Hinton Agar (MHA) media, which included Tetracycline, Gentamicin, Marbofloxacin, and Amoxicilin-Clavulanate antibiotic disks.

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Nurbaya, Siti, Yosy Cinthya Eriwaty Silalahi, Nurussakinah Nurussakinah, and Trionaldo Purba. "PENENTUAN KONSENTRASI HAMBAT MINIMUM (KHM) DARI EKSTRAK BUAH SAWO TERHADAP Salmonella typhi." JURNAL FARMANESIA 4, no. 2 (2017): 122–25. http://dx.doi.org/10.51544/jf.v4i2.2713.

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Sapodilla fruit (Manilkara zapota) belongs to the Sapotaceae family. Sapodilla fruit is traditionally used in traditional medicine to cure typhoid and sapodilla fruit is not only made medicine but also the sap is taken as raw material for making chewing gum. The purpose of the examination was to determine the minimum inhibitory concentration (MIC) of sapodilla fruit extract against Salmonella typhi bacteria. The method used is the agar diffusion method using Mueller Hinton Agar (MHA) as a growth medium and paper backing. The results of the examination of the effective inhibition at a concentra

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Noviyandri, Putri Rahmi, Diotama Nuza, and Suzanna Sungkar. "Effect of ethanol extract of robusta coffee leaves (Coffea canephora var. robusta) against Streptococcus mutans growth." Journal of Syiah Kuala Dentistry Society 5, no. 2 (2021): 75–79. http://dx.doi.org/10.24815/jds.v5i2.20017.

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The study to determine the inhibition capability of robusta coffee's (Coffea canephora var. robusta) leaf extract against S. mutans growth. Robusta coffee's (Coffea canephora var. robusta) leaf extract was made by maceration method using 96% ethanol. The antibacterial examination using well diffusion on Mueller Hinton Agar (MHA) media. The concentration of robusta coffee's (Coffea canephora var. robusta) leaf extract used in this study was 25%, 50%, and 75%. The study was analyzed using the One Way Analysis of Variance (ANOVA) test and continued with the Least Significant Difference (LSD) test

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Sapitri, Alfi, Eva Diansari Marbun, and Ulfayani Mayasari. "PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI." Jurnal Penelitian Saintek 26, no. 1 (2021): 64–73. http://dx.doi.org/10.21831/jps.v26i1.39859.

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Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terha

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Gading, Kalaudius. "UJI AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK DAUN MINDI (Melia azedarach L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis SECARA IN VITRO." INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL 5, no. 1 (2020): 8–18. http://dx.doi.org/10.52447/inspj.v5i1.1785.

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Jerawat merupakan salah satu penyakit infeksi yang dapat di sebabkan oleh bakteri S. epidermidis.Pada sebagian besar kasus infeksi, penggunaan antibiotik sangat diperlukan tetapi apabila pemakaian atntibiotik berlebihan akan menyebabkan bakteri menjadi resisten. Salah satu tanaman yang memiliki khasiat sebagai antibakteri adalah daun Mindi (Melia azedarach L.). Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antibakteri fraksi daun mindi (Melia azedarach L.) terhadap pertumbuhan bakteri Staphylococcus epidermidis. Metode yang digunakan adalah metode ekstraksi dan metode fraksi dan

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Tri Utami, Dini Fatwa, Muthia Zahra, Siti Fathia Azhar Hasibuan, and Yazima Fadila Ningsih. "Aktivitas Antibakteri Daun Mangga (Mangifera Indica L) Terhadap Bakteri Pada Sputum." Jurnal Biologi dan Pembelajarannya (JB&P) 11, no. 1 (2024): 11–17. https://doi.org/10.29407/jbp.v11i1.21571.

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Mango (Mangifera indica L.) is a native plant from Southeast Asia, and has been widely distributed in tropical and subtropical regions of the world. Mango leaf extract is reported to contain alkaloids, phenols, saponins, coumarins, tannins, flavonoids, triterponoids, steroids and glycosides which function as antimicrobial compounds that can inhibit bacterial growth. It is hoped that this content can be a solution as an antibacterial to replace antibiotics, because inappropriate use of antibiotics can cause bacteria to become resistant. The aim of this study was to test the antibacterial activi

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Supriani, Supriani, and Nurwijayanti Nurwijayanti. "Uji Kandungan Fitokimia dan Aktivitas Antibakteri dari Ekstrak Etanolik Bawang Dayak terhadap Bakteri Staphylococcus Aureus dalam Media Mueller Hinton Agar." Jurnal Farmasetis 10, no. 1 (2021): 1–6. http://dx.doi.org/10.32583/farmasetis.v10i1.955.

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Infeksi merupakan penyakityang disebabkan oleh bakteri. Antibakteri adalah zat yang dapat mengganggu pertumbuhan atau bahkan mematikan bakteri.Bawangdayak telah dilaporkan mengandung senyawa yang memiliki kemampuan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan uji aktivitas antibakteri dari ekstrak etanolik bawang dayak terhadap bakteri Staphylococcus aureus dalam media Mueller Hinton Agar (MHA).Metode:Metode yang digunakan untuk ekstraksi bawang dayak adalah maserasi, dengan pelarut etanol 96%. Ekstrak etanolik yang diperoleh diuji kandungan fitokimia

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Sundaresan, Preeja K., and Kala P. Kesavan. "In vitro evaluation of antibacterial activity in ethanolic extract of whole plant Sphaeranthus indicus Linn." International Journal of Basic & Clinical Pharmacology 9, no. 11 (2020): 1730. http://dx.doi.org/10.18203/2319-2003.ijbcp20204409.

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Background: Sphaeranthus indicus Linn is a widely used medicinal plant in Indian traditional system of medicine against human pathogens. Alarming bacterial resistance is urging scientist to search for newer anti-microbial substances from the medicinal plants. The objective of the study was to evaluate the antibacterial activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn (Asteraceae).Methods: The antibacterial activity of ethanolic extract of whole plant of Sphaeranthus indicus Linn was done against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphyloc

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Mansur, Murtala Musa, Jibril Mohammed Sani, Daneil Jesuwenu Ajoose, et al. "Antibacterial Activity of the Leaf Extract of Acalypha wilkesiana against some Human Bacterial Pathogens." UMYU Scientifica 3, no. 1 (2024): 168–76. http://dx.doi.org/10.56919/usci.2431.018.

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Humanity has long been creating traditional treatments based on the knowledge of medicinal plants found worldwide. The medicinal potential of Acalypha wilkesiana in treating skin and gastrointestinal problems is highly appreciated. This study aimed to examine the Acalypha wilkesiana leaf extract's antibacterial effectiveness against a variety of human bacterial infections. Standard techniques were used to determine whether secondary metabolites were present in methanolic, ethanolic, and hot water extracts. The plant extracts were evaluated for their ability to inhibit the growth of bacteria ob

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Putra, I. Made Agus Sunadi. "UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SIRSAK (Annonae muricata L.) DENGAN METODE DIFUSI AGAR CAKRAM TERHADAP Escherichia coli." Jurnal Ilmiah Medicamento 1, no. 1 (2020): 15–19. http://dx.doi.org/10.36733/medicamento.v1i1.721.

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Diare biasanya disebabkan oleh bakteri Eschericia coli yang terkontaminasi melalui makanan dan minuman yang terkontaminasi bakteri tersebut melalui lalat. Beberapa masyarakat menggunakan daun sirsak sebagai antidiare. Banyak kandungan yang terdapat dalam daun sirsak ini saponin, tanin, alkaloid, dan flavonoid, yang mana senyawa ini dapat berfungsi sebagai desinfektan-antiseptik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak etanol daun sirsak (Annonae muricatae L.) terhadap daya hambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan isolat bakter

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Nofita, Amalia Desty. "UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOLIK BAWANG MERAH (Allium cepa L.) TERHADAP BAKTERI Staphylococcus aureus DALAM MEDIA MUELLER HINTON AGAR (MHA)." Media Informasi 16, no. 1 (2021): 1–7. http://dx.doi.org/10.37160/bmi.v16i1.355.

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Normaliska, Ratna, Mirnawati Bachrum Sudarwanto, and Hadri Latif. "Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor." Acta VETERINARIA Indonesiana 7, no. 2 (2019): 42–48. http://dx.doi.org/10.29244/avi.7.2.42-48.

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Extended spectrum β-lactamase (ESBL) adalah enzim yang dapat menghidrolisis berbagai jenis antibiotik β-laktam termasuk generasi ketiga sefalosporin spektrum luas dan monobaktam. Tujuan penelitian ini adalah untuk melihat kepekaan dan resistensi Escherichia coli penghasil ESBL terhadap beberapa antibiotik. Isolat Escherichia coli penghasil ESBL (n=10) diisolasi dari total 80 sampel lingkungan di RPH-R Kota Bogor, sebelum proses produksi, menggunakan metode difusi cakram pada media Mueller Hinton Agar (MHA) dan interpretasi hasil mengacu pada Clinical and Laboratory Standards Institute (CLSI).

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Arsana, I. Nyoman, A. A. Ayu Sauca Sunia Widyantari, I. Ketut Winantra, Ni Putu Dyah Sartika Sari, Ni Made Ayu Suwandani, and Ni Ketut Ayu Juliasih. "Loloh Daun Kemangi (Ocimum basilicum L.) Tidak Cukup Kuat Sebagai Minuman Antioksidan Untuk Memperkuat Sistem Pengobatan Tradisional." Metamorfosa: Journal of Biological Sciences 11, no. 01 (2024): 73–81. http://dx.doi.org/10.24843/metamorfosa.2024.v11.i01.p7.

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Total Flavonoid dianalisis dengan menggunakan larutan standar quarsetine. Tanin dianalisis dengan menggunakan pereaksi Folin-Denis, aktivitas antioksidan dianalisis dengan menggunakan 2,2-diphenyl-1-picrylhydrazyl (DPPH) sebagai sumber radikal bebas, dan antibakteri dengan metode difusi pada Mueller Hinton Agar (MHA). Hasil penelitian menunjukkan total fenol, total flavonoid, tanin, dan IC50 loloh VB berturut-turut adalah 48,78 mgGAE/100g, 107,31 mg QE/100g, 72,24 mg/100 g, dan 3553,37 ppm. Sedangkan VL berturut-turut sebasar 91,40 mgGAE/100g, 305,65 mg QE/100g, 153,43mg/100g, dan 1507,85ppm.

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Yusro, Fathul, and Yeni Mariani. "The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis." Jurnal Biologi Tropis 25, no. 3 (2025): 2584–89. https://doi.org/10.29303/jbt.v25i3.9012.

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This study aims to evaluate the antibacterial activity of Melaleuca leucadendra (Gelam) bark extract against Pseudomonas aeruginosa and Enterococcus faecalis. Bark samples were collected from Karimunting Village, Bengkayang Regency, and extracted using maceration with 96% ethanol. Antibacterial testing was conducted using the disc diffusion method on MHA (Mueller Hinton Agar), with bacterial suspensions adjusted to McFarland standard 1. The results showed that the extract exhibited concentration-dependent antibacterial activity. The highest inhibition zones were observed at a concentration of

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Habibah, Firda Nurul, Hafizah Ilmi Sufa, Iis Kurniati, and Zuri Rismiarti. "Effect of solvent variation on results of antibiotic susceptibility test using the disk diffusion method against Staphylococcus aureus." Current Biomedicine 3, no. 2 (2025): 60–66. https://doi.org/10.29244/currbiomed.3.2.60.

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Background Mueller-Hinton agar (MHA) is widely used for disk diffusion tests to assess antibiotic susceptibility in non-fastidious bacteria. The type of water used to prepare the MHA may have affected the test outcomes. Objective This study evaluated the effect of different water types as solvents on the antibiotic susceptibility test results of tetracycline and gentamicin against Staphylococcus aureus. Methods MHA was prepared using four types of water: distilled (control), bottled, reverse osmosis (RO), and municipal tap water (PDAM). Antibiotic susceptibility testing was performed using the

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Chairunas, Chairunas, Gian Salsabila Sinta, Liza Meutia Sari, Rachmad Suhanda, and Siti Nur Nabihah Zainul Abidin. "Bacterial growth assessment of extraction pliers following tooth extraction in culturing bacterial on various media: a quasi-experimental study." Padjadjaran Journal of Dentistry 36, no. 2 (2024): 224–31. http://dx.doi.org/10.24198/pjd.vol36no2.53803.

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ABSTRACTIntroduction: Tooth extraction injury potentially creating an entry point for bacteria that may cause infection. Dental extraction tools that come into contact with a patient's oral cavity contain many bacteria which can be opportunistic and pathogenic. The purpose of this study was to examine the bacterial growth on tooth extraction pliers following tooth extraction in culturing bacteria on various media. Methods: Type of study is a quasi-experimental research design, using pre and post-test analysis. It was conducted in September–October 2021 at the Teeth and Mouth Dentistry Hospital

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Susanti, Novi, Dini Oktaviani, Leni Apriani, and Gustriana Gustriana. "effectivity of Garlic (Allium sativum L.) and Turmeric (Curcuma longa L.) in Tilapia (Oreochromis niloticus) infected by Edwardsiella tarda." IJOTA (Indonesian Journal of Tropical Aquatic) 4, no. 2 (2022): 1–12. http://dx.doi.org/10.22219/ijota.v4i2.17961.

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The aim of this study is to determine the effectivity of Garlic (Allium sativum L.) dan Turmeric (Curcuma longa L.) in Tilapia (Oreochromis niloticus) infected by Edwardsiella tarda. We used sensitivity test with disc diffusion Kirby-Bauer method. The 24 hours purified E. tarda isolate, cultivated in Mueller Hinton Agar (MHA) by swabbing it using a sterile swab on the MHA surface, next put paper disc contain variants garlic concentrations and turmeric. Incubated MHA on the room temperature (30 oC) for at least 24 hours, inhibition zone diameter is measured to see the effectivity. Serial Tube D

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Sari, Devi Indah, Retno Sri Wahjuni, Ratih Novita Praja, Budi Utomo, Faisal Fikri, and Prima Ayu Wibawati. "Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro." Jurnal Medik Veteriner 4, no. 1 (2021): 63. http://dx.doi.org/10.20473/jmv.vol4.iss1.2021.63-71.

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The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions

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Hubert, Susannah K., Jasmine M. Mohammed, Scott K. Fridkin, Robert P. Gaynes, John E. McGowan, and Fred C. Tenover. "Glycopeptide-Intermediate Staphylococcus aureus: Evaluation of a Novel Screening Method and Results of a Survey of Selected U.S. Hospitals." Journal of Clinical Microbiology 37, no. 11 (1999): 3590–93. http://dx.doi.org/10.1128/jcm.37.11.3590-3593.1999.

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Isolates of Staphylococcus aureus with decreased susceptibilities to glycopeptide antimicrobial agents, such as vancomycin and teicoplanin, have emerged in the United States and elsewhere. Commercially prepared brain heart infusion agar (BHIA) supplemented with 6 μg of vancomycin per ml was shown in a previous study to detect glycopeptide-intermediate S. aureus (GISA) with high sensitivity and specificity; however, this medium, when prepared in-house, occasionally showed growth of vancomycin-susceptible control organisms. This limitation could significantly impact laboratories that prepare med

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Dixit, Manju, and Megha Tiwari. "Study of Antimicrobial Quality of Tobacco Leaves Extract (Nicotiana tabacum) on Aeromonas hydrophila and Escherichia coli." Biosciences Biotechnology Research Asia 20, no. 4 (2023): 1461–65. http://dx.doi.org/10.13005/bbra/3191.

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ABSTRACT: Tobacco is a perennial herb and its belong to family Solanaceae. Tobacco leaves have some antimicrobial substance such as alkaloids, phenol and some essential oils. The leaves extract of this plant show some microbial Quality and influence on both gram positive and gram negative bacteria. The aim of this work to determine the effectiveness of tobacco (Nicotiana tabacum) leaves extract in inhibiting on Aeromonas hydrophila and Escherichia coli. The antibacterial test performed on Mueller Hinton Agar (MHA) media. Test result indicate the leaves extract of tobacco showed positive result

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Admi, Masda, Wilda Kurniawan, and Dasrul Dasrul. "Isolasi, Identifikasi dan Uji Sensitivitas Bakteri Streptococcus SP. Penyebab Endometritis Subklinis pada Sapi Aceh." Jurnal Peternakan 21, no. 2 (2024): 145. http://dx.doi.org/10.24014/jupet.v21i2.24172.

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ABSTRAK. Penelitian ini bertujuan untuk isolasi, identifikasi dan uji sensitivitas antibiotik eritromisin dan penisilin G terhadap bakteri Streptococcus sp. penyebab endometritis subklinis pada sapi aceh. Penelitian ini dilakukan dengan cara swab dibagian vagina sapi sebagai sampel, dan dimasukkan ke dalam tabung reaksi berisi Nutrien Broth (NB), kemudian diisolasi menggunakan media Manitol Salt Agar (MSA) dan diidentifikasi koloni bakteri menggunakan pewarnaan Gram, serta dilakukan uji katalase. Hasil isolasi bakteri tersebut diuji sensitivitas antibiotik dengan cara menempelkan disk antibiot

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Nur Laela Alydrus and Nurul Khofifahl. "Efektifitas Antibakteri Ekstrak Daun Sirih Hijau (Piper Betle L) Terhadap Staphylococcus Aureus." INHEALTH : INDONESIAN HEALTH JOURNAL 1, no. 1 (2022): 56–61. http://dx.doi.org/10.56314/inhealth.v1i1.23.

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Daun sirih hijau (Piper betle L) sudah lama digunakan oleh warga Indonesia selaku obat tradisional. Ekstrak daun sirih hijau memiliki energi antibakteri yang terdiri dari fenol serta senyawa turunannya yang membatasi bermacam berbagai perkembangan Bakteri. Penelitian ini dilakukan untuk mengetahui Efektifitas Antibakteri ekstrak Daun sirih hijau (Piper betle L) Terhadap Staphylococcus aureus. Dan mengetahui efektifitas dari beberapa konsentrasi 20%, 40%, 60%, dan 80% dalam meghambat pertumbuhan Staphylococcus aureus. untuk uji zona hambat pada pertumbuhan Staphyloccocus aures dengn menggunakan

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Anwar, Eka Nurdianty, Wenda Permita Sari, Parwito Parwito, and Ismail Arifin. "Uji Daya Hambat Air Rebusan Daun Sirih Hijau (Piper Betle L.) Terhadap Bakteri Staphylococcus Aureus." Jurnal Ilmiah Pharmacy 10, no. 2 (2023): 173–79. https://doi.org/10.52161/jiphar.v10i2.617.

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Telah dilakukan penelitian tentang Uji Daya Hambat Air Rebusan Daun Sirih Hijau (Piper betle L) terhadap bakteri Staphylococcus aureus pada tanggal 20-24 Februari 2023 di Laboratorium Bakteri Kampus Akademi Analis Kesehatan Harapan Bangsa, Bengkulu. Penelitian ini bertujuan untuk mengetahui Air Rebusan Daun Sirih Hijau (Piper betle L) dapat menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini menggunakan metode Difusi dan Dilusi. Teknik pengambilan sampel menggunakan purposive sampling. Sampel yang digunakan dalam penelitian ini adalah Air Rebusan Daun Sirih Hijau (Piper betle

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Primasari, Ameta, Minasari Nasution, Nurul Hidayati Arbi, Dini Permata Sari, and Mohammad Basyuni. "THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO." Asian Journal of Pharmaceutical and Clinical Research 11, no. 12 (2018): 411. http://dx.doi.org/10.22159/ajpcr.2018.v11i12.28435.

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Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Eac

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Halkai, Rahul, Kiran R. Halkai, Syeda Uzma Mahveen, Syed Zakaullah, S. Syed Ishaq, and Javeria Firdous. "Evaluation of antimicrobial efficacy of 1% phytic acid incorporated with 0.2% chitosan nanoparticles against Enterococcus faecalis: An in vitro study." Endodontology 35, no. 3 (2023): 248–53. http://dx.doi.org/10.4103/endo.endo_238_22.

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ABSTRACT Aim: The aim of the study was to evaluate the antimicrobial activity of 1% phytic acid and ethylenediaminetetraacetic acid (EDTA) incorporated with 0.2% of chitosan nanoparticles (Csnps) against Enterococcus faecalis (E. faecalis) by agar diffusion and biofilm model. Materials and Methods: Antimicrobial efficacy was evaluated in two stages: (i) agar well-diffusion method and (ii) biofilm model. E faecalis (American Type Culture Collection 29212) strain was subcultured and grown on the blood agar plates, followed by placement of test solutions in punched holes (5 mm diameter) and incub

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Unita, Lisna, and Colvin Voon. "DAYA HAMBAT EKSTRAK DAUN KARI TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus." Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) 10, no. 3 (2018): 287–91. http://dx.doi.org/10.36911/pannmed.v10i3.117.

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Curry leaves ( Murraya koenigii ) can be used as a spice in cooking and curry leaf extract , has the effect of antimicrobial activity can inhibit the growth of Staphylococcus aureus . The purpose of this study was to determine the curry leaf extract solution of various concentrations to inhibit the growth of Staphylococcus aureus and know the difference inhibition curry leaf extract solution between the various different concentrations on the growth of Staphylococcus aureus . This study uses laboratory Experimental design by Design Posttest only control group design . Test method using agar di

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Pratiwi, Siska Telly, Sayu Putu Yuni Paryati, Elizabeth Noviana Lumban Raja, and Panca Andana. "PERBANDINGAN EFEKTIVITAS BAKTERIOSIN Lactobacillus acidophillus ATCC 4356 DENGAN NISIN PADA PERTUMBUHAN Salmonella typhi ATCC 6539." Medika Kartika Jurnal Kedokteran dan Kesehatan, Volume 5 No 2 (June 30, 2022): 162–74. http://dx.doi.org/10.35990/mk.v5n2.p162-174.

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Demam tifoid termasuk penyakit infeksi serius di Indonesia. Permasalahan yang sering timbul sehubungan penanganan kasus demam tifoid salah satunya adalah resistensi antibiotik. Resistensi antibiotika menjadi masalah global terutama dalam menangani masalah infeksi. WHO melaporkan Asia Tenggara memiliki angka tertinggi dalam kasus resistensi antibiotik di dunia. Penggunaan antimikroba yang tidak rasional dan berkembangnya resistensi antibiotika membuka ketertarikan untuk menggunakan alternatif antimikroba alami seperti bakteriosin. Bakteri Lactobacillus acidophilus (L. acidophilus) menghasilkan

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Sari, Wahyu E., Muhammad Hambal, Henni Vanda, et al. "In Vitro Evaluation of Antimicrobial Activity of Coffee Grounds Extracts against Fish Pathogenic Aeromonas hydrophila." E3S Web of Conferences 151 (2020): 01042. http://dx.doi.org/10.1051/e3sconf/202015101042.

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Fish diseases caused bypathogenic bacteria Aeromonas hydrophila is one of the obstacles for fish farming because it can cause death and loss for farmers. A. hydrophila was reported to possess resistance to several antibiotics. Therefore, there is a need to seek an alternative source of natural antimicrobials. This in vitro study aimed to examinethe effect of ethanol extracts of coffee grounds as an antimicrobial to A. hydrophila which infects carp (Cyprinus carpio). The coffee grounds were collected from four traditional coffee shops in Banda Aceh, and a total of 500 g coffee grounds were extr

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Elobaid, Rahaf, Bhupinder Kaur, and Natesan Vijayalakshmi. "Isolation and identification of Bifidobacterium from dairy products and screening its antibacterial activity." QIJMHS 3, no. 2 (2020): 38–43. https://doi.org/10.5281/zenodo.4473214.

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<strong>Introduction:</strong> <em>Bifidobacterium</em> is one of the most used probiotic microorganisms in the food industry due to its health-enhancing benefits. <strong>Methods:</strong> In this study, twenty samples from five different dairy products were collected. <em>Bifidobacterium</em> was isolated on Man Rogosa Sharpe (MRS) agar and identified through several tests, including Gram staining, catalase test, oxidase test, triple sugar iron test and testing its susceptibility to mupirocin. Antimicrobial activity against 3 Gram-negative and 1 Gram-positive bacteria which includes <em>Pseu

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Sengupta, Mallika, Sayantan Banerjee, and Tanoy K. Sarker. "Identification of Fosfomycin Heteroresistance Among Multidrug-Resistant Enterobacteriaceae Urinary Isolates." Recent Advances in Biology and Medicine 6 (2020): 1. http://dx.doi.org/10.18639/rabm.2020.1117679.

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Fosfomycin is an orally bioavailable bactericidal agent which concentrates significantly in urine and is being increasingly prescribed for patients with Multidrug Resistant Enterobacteriaceae (MDRE) urinary isolates. According to the Clinical and Laboratory Standards Institute (CLSI), disc diffusion (DD) and agar dilution (AD) are the standards for fosfomycin susceptibility testing for urinary Escherichia coli and Enterococcus faecalis isolates, whereas the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines suggest both AD and broth microdilution (BD), but not DD. A

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Chairani, Aulia, and Erna Harfiani. "Efektivitas Getah Jarak Sebagai Antiseptik terhadap Pertumbuhan Staphylococcus aureus, Escherichia colidan Candida sp. secara In Vitro." Jurnal Kedokteran Universitas Lampung 2, no. 2 (2018): 84–92. https://doi.org/10.23960/jkunila.v2i2.pp84-92.

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Kulit manusia merupakan perlindungan utama terhadap berbagai jenis mikroorganisme yang menyerang imun tubuh. Apabila terjadi kerusakan pada kulit seperti luka, maka terbukalah sistem pertahanan yang kompleks pada kulit. Larutan antiseptik diharapkan dapat membunuh mikroorganisme di sekitar luka agar tidak menyebabkan gangguan lebih lanjut. Salah satu tanaman yang mempunyai zat bioaktif alkaloid, flavonoid, tannin dan saponin yang dapat dipergunakan sebagai antiseptik adalah getah tanaman jarak cina (Jatropha multifida L.).Penelitian ini dilakukan menggunakan metode eksperimental murni, bertuju

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Fitriyanti, Fitriyanti, Abdurrazaq Abdurrazaq, and Muhammad Nazarudin. "UJI EFEKTIVITAS ANTIBAKTERI ESKTRAK ETIL ASETAT BAWANG DAYAK (Eleutherine palmifolia Merr) TERHADAP STAPHYLOCOCCUS AUREUS DENGAN METODE SUMURAN." Jurnal Ilmiah Manuntung 5, no. 2 (2020): 174. http://dx.doi.org/10.51352/jim.v5i2.278.

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Dayak onion (Eleutherine palmifolia Merr.) is a multifunctional medicinal plant for various diseases, one of which is an antibacterial. Dayak Onions (E. palmifolia Merr.) contains an alkaloid, flavonoid, triterpenoid, and saponin compound which has antibacterial properties. This study aims to determine the effect of the ethyl acetate extract of Dayak Onion tubers inhibits the growth of Staphylococcus aureus bacteria. Dayak onion tuber was extracted using ethyl acetate solvent by the maceration method until thick extract was obtained. The concentration extract used were 30 mg/ml;15 mg/ml;7,5 mg

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Sulistiawati Dewi, Putu, and Setiawan Setiawan. "RHIZOME BANGLE EXTRACT INHIBITION GROWTH TEST AGAINST Staphylococcus aureus FOR ORAL ABSCESSS HERBAL TREATMENT (UJI DAYA HAMBAT EKSTRAK RIMPANG BANGLE TERHADAP PERTUMBUHAN Staphylococcus aureus SEBAGAI PERAWATAN HERBAL ABSES ORAL)." Journal of Health and Dental Sciences 1, Volume 1 No 3 (2021): 225–32. http://dx.doi.org/10.54052/jhds.v1n3.p225-232.

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An abscess is a typical infection caused by Staphylococcus aureus bacteria. Resistance of bacteria occurs due to improper antibiotics dosage, inaccurate diagnosis, and improper bacterial causes. Rhizome Bangle contains saponins, flavonoids and essential oils, alkaloids, tannins, and glycosides that have antibacterial. The purpose of this study is to determine the inhibitory effect of rhizome Bangle extract (Zingiber purpureum Roxb.) on the growth of Staphylococcus aureus bacteria in Vitro. The study was an experimental laboratory study with group design post-test in vitro. The method used as t

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