Academic literature on the topic 'Microalgae biofuels'

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Journal articles on the topic "Microalgae biofuels"

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Zhu, Lian Dong, Erkki Hiltunen, and Josu Takala. "Microalgal Biofuels Beat the First and Second Generation Biofuels." Applied Mechanics and Materials 197 (September 2012): 760–63. http://dx.doi.org/10.4028/www.scientific.net/amm.197.760.

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Recently biofuels derived from biomass have received increased concerns in an attempt to search for sustainable development. The first and second generation biofuels are unsustainable since the growth of these food or non-food crops for biofuel generation will compete for limited arable farmlands, thus increasing the risks on food availability. Microalgal biofuels, known as the third generation biofuels, have the potential for sustainable production in an economically effective manner. The advantages of microalgae as a biofuel feedstock are many, for instance, high photosynthesis efficiency, high oil content and noncompetition with food crop production on farmlands. Microalgae can be employed for the production of biodiesel, bioethanol, biogas, biohydrogen, among others. The integrated biorefinery approach has huge potential to greatly improve the economics of biofuel production from microalgae. However, the production of microalgal biofuels is still at pre-commercial stages since it is expensive to produce substantial amount of biofuels at a large scale. Despite this, microalgae are still the most promising and best feedstock available for the biofuels. Biotechnology advances including genetic and metabolic engineering, well-funded R&D researches and policy support can make microalgal biofuels have a bright future.
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Bhatt, Neha Chamoli, Amit Panwar, Tara Singh Bisht, and Sushma Tamta. "Coupling of Algal Biofuel Production with Wastewater." Scientific World Journal 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/210504.

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Microalgae have gained enormous consideration from scientific community worldwide emerging as a viable feedstock for a renewable energy source virtually being carbon neutral, high lipid content, and comparatively more advantageous to other sources of biofuels. Although microalgae are seen as a valuable source in majority part of the world for production of biofuels and bioproducts, still they are unable to accomplish sustainable large-scale algal biofuel production. Wastewater has organic and inorganic supplements required for algal growth. The coupling of microalgae with wastewater is an effective way of waste remediation and a cost-effective microalgal biofuel production. In this review article, we will primarily discuss the possibilities and current scenario regarding coupling of microalgal cultivation with biofuel production emphasizing recent progress in this area.
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Assadad, Luthfi, Bagus Sediadi Bandol Utomo, and Rodiah Nurbaya Sari. "The use of microalgae as the raw material of bioethanol." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 5, no. 2 (August 1, 2010): 51. http://dx.doi.org/10.15578/squalen.v5i2.47.

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Biofuel is one of alternative fossil fuel, in which the raw materials come from biological resources.One of the raw materials for biofuel production is microalgae. Microalgae grows rapidly, does notcompete with food for humans, and needs small areas to cultivate. Utilization of microalgae forbiofuel research nowadays is focusing on biodiesel production, but actually microalgae can beused to produce other biofuels such as bioethanol. The carbohydrate content of the microalgaecan be converted into glucose and fermented into alcohol. Carbohydrate content of the microalgaeis about 5.0–67.9%, which could produce bioethanol up to 38%. A harmony between bioethanoland biodiesel production from microalgae is needed for the optimum utilization of microalgae.Bioethanol production from microalgae can be done using de-oiled microalgae.
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Ortiz-Marquez, Juan Cesar Federico, Mauro Do Nascimento, Maria de los Angeles Dublan, and Leonardo Curatti. "Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae." Applied and Environmental Microbiology 78, no. 7 (January 20, 2012): 2345–52. http://dx.doi.org/10.1128/aem.06260-11.

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ABSTRACTConcerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third-generation biofuels from microalgal biomass mainly because of its potential for high yields and reduced land use changes in comparison with biofuels derived from plant feedstocks. Regardless of the nature of the feedstock, the use of fertilizers, especially nitrogen, entails a potential economic and environmental drawback for the sustainability of biofuel production. In this work, we have studied the possibility of nitrogen biofertilization by diazotrophic bacteria applied to cultured microalgae as a promising feedstock for next-generation biofuels. We have obtained anAzotobacter vinelandiimutant strain that accumulates several times more ammonium in culture medium than wild-type cells. The ammonium excreted by the mutant cells is bioavailable to promote the growth of nondiazotrophic microalgae. Moreover, this synthetic symbiosis was able to produce an oil-rich microalgal biomass using both carbon and nitrogen from the air. This work provides a proof of concept that artificial symbiosis may be considered an alternative strategy for the low-N-intensive cultivation of microalgae for the sustainable production of next-generation biofuels and other bioproducts.
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Jimenez Escobedo, Manuel, and Augusto Castillo Calderón. "Microalgal biomass with high potential for the biofuels production." Scientia Agropecuaria 12, no. 2 (June 1, 2021): 265–82. http://dx.doi.org/10.17268/sci.agropecu.2021.030.

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The study of biofuels continues in constant development, for five decades. This article summarizes the analysis of several recent scientific publications, related to third generation biofuels using microalgae. An overview of biofuels and their classification, the theoretical bases of microalgae, techniques for their cultivation, harvesting and pretreatment of their biomass are presented. Promising technologies for obtaining biofuels of great potential worldwide demand are also briefly described, considering the technical characteristics of the process, depending on the microalgae species that have the highest yields and productivity for each type of biofuel:Biodiesel (extraction of lipids, transesterification and purification), ethanol (hydrolysis of sugars, fermentation and purification) and biogas (anaerobic digestion).Most studies are focused on the production of lipids, being Chlorella vulgaris, Nanochloropsis sp. and Botryococcus braunii(A) the most used microalgae to obtain biodiesel. However, there are few studies focused on the production of microalgal biomass toproduce bioethanol, thus, the microalgae Porphyridium cruentumand Spirogira sp. they could be used to produce bioethanol, with the advantage of not containing lignin. Biogas is produced by anaerobic biodigestion of microalgal biomass residues in biorefineries, but its commercial production is very limited due to high production costs and because there are other economically very competitive biomasses. The need to produce biofuels using microalgal biomass is reaching a greater boom, the transcendental proposal being the launching of a biorefinery, mainly focused on the optimal production of microalgal biomass as the main key to the entire process.
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Jayaseelan, Merrylin, Mohamed Usman, Adishkumar Somanathan, Sivashanmugam Palani, Gunasekaran Muniappan, and Rajesh Banu Jeyakumar. "Microalgal Production of Biofuels Integrated with Wastewater Treatment." Sustainability 13, no. 16 (August 6, 2021): 8797. http://dx.doi.org/10.3390/su13168797.

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Human civilization will need to reduce its impacts on air and water quality and reduce its use of fossil fuels in order to advance towards a more sustainable future. Using microalgae to treat wastewater as well as simultaneously produce biofuels is one of the approaches for a sustainable future. The manufacture of biofuels from microalgae is one of the next-generation biofuel solutions that has recently received a lot of interest, as it can remove nutrients from the wastewater whilst capturing carbon dioxide from the atmosphere. The resulting biomass are employed to generate biofuels, which can run fuel cell vehicles of zero emission, power combustion engines and power plants. By cultivating microalgae in wastewater, eutrophication can be prevented, thereby enhancing the quality of the effluent. Thus, by combining wastewater treatment and biofuel production, the cost of the biofuels, as well as the environmental hazards, can be minimized, as there is a supply of free and already available nutrients and water. In this article, the steps involved to generate the various biofuels through microalgae are detailed.
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Siti Zulaiha. "Genetic engineering of microalgae lipid biosynthesis for sustainable biodiesel production." World Journal of Advanced Research and Reviews 11, no. 3 (September 30, 2021): 072–77. http://dx.doi.org/10.30574/wjarr.2021.11.3.0397.

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Biofuel is one of the most promising alternative energy sources for reducing human reliance on fossil fuels. Microalgae has recently emerged as the most promising biofuel source. However, biofuels from microalgae are still not feasible to replace fossil fuels because of their high production costs, therefore, it is necessary to pick microalgae species with high growth rates and lipid content. Overexpression of lipid biosynthesis enzymes and inhibition of competitive metabolic pathways are two genetic engineering strategies that can be developed to assess microalgae lipid production. Malate and multienzyme enzymes (GPAT, LPAAT and DGAT) can be overexpressed in microalgae to boost lipid production. The strategy of blocking competitive metabolic pathways can be carried out through suppression of starch metabolism and lipid catabolism. The strategy of blocking competitive metabolic pathways has been carried out in several microalgae and is effective for enhancing lipid biosynthesis. Several mutations that block both the starch metabolic and lipid catabolic pathways can result in increased levels of microalgal lipid accumulation.
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Blinová, Lenka, Alica Bartošová, and Kristína Gerulová. "Cultivation Of Microalgae (Chlorella vulgaris) For Biodiesel Production." Research Papers Faculty of Materials Science and Technology Slovak University of Technology 23, no. 36 (June 1, 2015): 87–95. http://dx.doi.org/10.1515/rput-2015-0010.

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Abstract Production of biofuel from renewable sources is considered to be one of the most sustainable alternatives to petroleum sourced fuels. Biofuels are also viable means of environmental and economic sustainability. Biofuels are divided into four generations, depending on the type of biomass used for biofuels production. At present, microalgae are presented as an ideal third generation biofuel feedstock because of their rapid growth rate. They also do not compete with food or feed crops, and can be produced on non-arable land. Cultivation conditions (temperature, pH, light, nutrient quantity and quality, salinity, aerating) are the major factors that influence photosynthesis activity and behaviour of the microalgae growth rate. In this paper, we present an overview about the effect of cultivation conditions on microalgae growth.
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Lourenço, S. O. "EDITORIAL." Revista de Engenharia Térmica 8, no. 1 (June 30, 2009): 02. http://dx.doi.org/10.5380/reterm.v8i1.61858.

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The search for energy sources that alleviate the dependency on fossil fuels is one the greatest challenges of humankind. The environmental damages that result of many decades of gas emissions from burning oil, natural gas, and mineral coal are evident, revealed by the high levels of atmospheric CO2 and by the ocean acidification, for instance. Two fundamental routes will help to reduce the dependence on fossil fuels: the development of machines and engines with more efficient consumption of fuel and the production of renewable sources of energy, such as biofuels.Brazil is probably the country with the highest potential to produce biofuels. The Brazilian success in the production of ethanol since the 1970’s is a world landmark. The recent growth of biodiesel production in Brazil from different sources (e.g., soybeans, bovine fat) is encouraging. New matrixes to produce biodiesel have been tested all over the world. Microalgae represent a world hope to generate advanced biofuels, allying a (potential) huge scale and very high productivity.In theory, microalgae can triplicate their biomass in 24 hours, depending on the species. This high growth rate combined to high accumulation of triglycerides allow the estimates that some microalgae could generate dozens of thousands of liters of biodiesel / ha per year. Microalgae do not follow seasonal crop harvest regimes (they can be harvested on daily basis), they make biofixation of CO2, occupy small physical areas, and can be cultivated in salty or brackish waters, avoiding the competition with scarce water resources for human consumption of irrigation. Fertile lands are unnecessary, since the cultivation includes ponds or photobioreactors, which are independent of the soil characteristics. There is no conflict with land use for agriculture, deforestation of pristine biomes is avoided, and there is the possibility to generate valuable co-products in parallel to biofuel production.Despite these stimulating arguments, no company produces biofuel from microalgae at commercial scale. Several hurdles still have to be overcome, such as the cost and the efficiency of the separation of the cells from the liquid medium, the accumulation of more triglycerides by the microalgae, the reduction of costs of the systems for mixing the cultivation and dissolution of CO2, and the scarce availability of water in key regions, among others. All technical problems put together and the high intensity of manpower result in high costs of production of biofuels from microalgae. Probably it is not possible yet to produce 1 liter of microalgae biodiesel for less than US$ 9.00, a value that makes the incorporation of microalgae to the world matrix of biofuel to be economically impossible, using the current technology.Due to the Brazilian tradition on biofuels, there is a tremendous international expectation on the participation of Brazil in the production of biofuels from microalgae. Several Brazilian groups have been working on the challenge of creating solutions to make feasible the cultivation of microalgae to generate biofuels. In the previous issue of Engenharia Térmica, two good examples of the Brazilian effort to develop microalgae production can be evaluated by the readers. Ribeiro et al. offered a mathematical analysis of the growth of Phaeodactylum tricornutum, a fast-growing marine microalga, in a closed system for cultivation - a photobioreactor. Torrens et al. evaluated the properties of different kinds of biodiesel generated from microalgae and their theoretical gas emissions in engines, based on the characteristics of their fatty acid composition. These initiatives are important and very welcome. Hopefully, these promising results will stimulate the development of the field in the country, attract more researchers to the subject, and inspire the cooperation amongmultidisciplinary Brazilian teams.
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Blinová, Lenka, Alica Bartošová, and Maroš Sirotiak. "Unconventional Type of Biomass Suitable for the Production of Biofuels." Advanced Materials Research 860-863 (December 2013): 514–17. http://dx.doi.org/10.4028/www.scientific.net/amr.860-863.514.

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Production of biofuel from renewable sources is considered to be one of the most sustainable alternatives to petroleum sourced fuels. Biofuels are also viable means for environmental and economic sustainability. Biofuels are divided into four generations. At present microalgae are presented as an ideal third generation biofuel feedstock because of their rapid growth rate and they also do not compete with food or feed crops, and can be produced on non-arable land. Microalgae have broad bioenergy potential because they can be used to produce liquid transportation and heating fuels (bioethanol, biodiesel). In this paper we present an overview about biofuels generation, especially about using duckweed for bioethanol production.
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Dissertations / Theses on the topic "Microalgae biofuels"

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Canter, Christina Elizabeth. "The Sustainability of Biofuels Produced from Microalgae." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/293419.

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Fossil fuels are not sustainable due to their worldwide depletion and greenhouse gas (GHG) emissions. Transportation biofuels produced from microalgae are sustainable if GHG emissions are lower than fossil fuels and the sources for materials used during production are sustainable. Four areas were evaluated to address these concerns. First, a study of peer reviewed life-cycle analyses (LCAs) was performed. The purpose of this evaluation was to determine which processing choices during cultivation have the most impacts. Data from nine authors was converted to similar units, and a new LCA was performed to evaluate the impacts. Overall GHG emissions per kg of algae cultivation ranged from 0.1 - 4.4 kg CO₂ eq. / kg algae, with the most of the emissions coming from fertilizer contributions. The second topic evaluated was the GHG emissions from experimental dewatering technologies. The five experimental technology emissions, for acoustic harvesting, membrane filtration, flocculation, electrocoagulation and flocculation plus belt filtration, were compared to a modeled dissolved air flotation technology and a fossil fuel source. For a functional unit of one MJ of renewable diesel (RD), membrane filtration had the lowest GHG emissions at 40.8 g CO₂(eq)/MJ RD. Dissolved air flotation was the highest scenario at 51.9 g CO₂(eq)/MJ RD. All technologies were lower than gasoline at 90.7 g CO₂(eq)/MJ gasoline. The third topic evaluated was the GHG emissions from the materials used for plant construction. A LCA was performed for the infrastructure materials and compared to results from the fuel-cycle. Plastic from pond liners had the largest contribution to GHG emissions for the baseline case. Increasing productivity and lipid content both decreased infrastructure emissions. The final topic evaluated was the sustainability of nitrogen, phosphorus and potassium used for microalgae growth. Results show that the surplus of world fertilizers cannot sustain large scale algae production in the United States. Technology choices that can recycle nutrients lower the overall requirement. Alternative sources of nutrients, like concentrated animal feeding operations, can provide enough nutrients for large scale production of algae.
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Al, Emara Mohammed-Hassan Khairallah. "Microalgae cultivation and harvesting for the production of biofuels." Thesis, University of Surrey, 2017. http://epubs.surrey.ac.uk/813963/.

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Increasing concern over climate change and the impact of greenhouse gas emissions as well as diminishing global oil reserves has pushed research into alternative energy. Reducing the cost of microalgae, a promising source for alternative energy, is a key step in commercialising biodiesel production. Currently avenues such as the use of waste stream cost effective cultivation system and efficient harvesting options are being explored for the common goal of establishing commercially viable microalgae production and utilisation schemes. From reviewing the current progress presented in literature this research has identified several aspects of importance to commercialising biofuel production. After identifying several gaps in the literature covering direct comparison of microalgal biomass production between temperate and hot region, a novel investigation utilising a refined computer model was undertaken to compare upstream cultivation of open systems in both temperate and hot climates. The outcome of which suggested the relative importance of light over temperature for the cultivation of microalgae in an open pond system. This was then explored further experimentally by setting the temperate light intensity, photoperiod and temperature conditions for three months representing summer and winter seasons. The results of this novel adaptation of seasonal highs and lows data of a temperate climate (UK) indicated that a more effective direction of intervention is the investment in additional light-supply in place of a heating-system, which is more than likely to yield higher algal biomass for biofuel production. Finally, an approach was made towards engaging more economical aspects of the process from upstream cultivation of waste stream based nutrients (leachate) with a native microalgae strain for the first time, to downstream dewatering of algal biomass with innovative improvements to energy efficient forward osmosis technology by uniquely assessing microalgae nutrient-based draw solution. The results both indicated the real potential of utilising these cost efficient methods at a lab scale. The ultimate goal of the project was to combine the research efforts for both cultivation (upstream) and harvesting (downstream) to assist in the understanding of the commercial viability of biofuel production from microalgae.
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Baroukh, Caroline. "Metabolic modelling under non-balanced growth : application to microalgae growth for biofuels production." Thesis, Montpellier 2, 2014. http://www.theses.fr/2014MON20190.

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La modélisation métabolique est un outil performant pour mieux comprendre, prédire et optimiser les bioprocédés, particulièrement lorsqu'ils impliquent des molécules d'intérêt. Malheureusement, l'utilisation de cette approche de modélisation pour des métabolismes dynamiques est difficile à cause du manque de données expérimentales nécessaires pour définir et calibrer les cinétiques des réactions appartenant aux chemins métaboliques. C'est pourquoi, les modèles métaboliques sont souvent utilisés sous l'hypothèse de croissance équilibrée. Cependant, pour certains procédés comme la croissance photoautotrophique des microalgues, l'hypothèse de croissance équilibrée ne semble pas raisonnable à cause de la synchronisation de leur cycle circadien sur la lumière du jour. Cependant, une compréhension approfondie du métabolisme des microalgues est nécessaire afin d'optimiser les rendements de production des bioprocédés basés sur ces microorganismes, comme par exemple la production de biocarburants.Dans cette thèse, DRUM, une nouvelle approche de modélisation métabolique dynamique qui prend en compte la croissance non-équilibrée, a été développée. La première étape de l'approche consiste à découper le réseau métabolique en sous-réseaux décrivant des réactions qui sont spatialement et fonctionnellement proches et supposés satisfaire une croissance équilibrée. Les métabolites interconnectant les sous-réseaux peuvent alors avoir un comportement dynamique. Puis, grâce à l'analyse de modes élémentaires, chaque sous-réseau est réduit à des réactions macroscopiques, pour lesquelles des cinétiques simples sont supposées. Enfin, un système d'équations ordinaires différentielles est obtenu pour décrire la consommation des substrats, la production de biomasse, les produits excrétés et l'accumulation de certains métabolites intracellulaires.DRUM a été appliquée à l'accumulation des lipides et des carbohydrates de la microalgue Tisochrysis lutea soumise à des cycles jour/nuits en condition d'azote normal et de carence azotée. Le model décrit avec précision les données expérimentales. DRUM a également été appliquée à la microalgue Chlorella Sorokiniana en croissance hétérotrophique, montrant que la croissance équilibrée est valide dans ce cas-là
Metabolic modeling is a powerful tool to understand, predict and optimize bioprocesses, particularly when they imply intracellular molecules of interest. Unfortunately, the use of metabolic models for time varying metabolic fluxes is hampered by the lack of experimental data required to define and calibrate the kinetic reaction rates of the metabolic pathways. For this reason, metabolic models are often used under the balanced growth hypothesis. However, for some processes such as the photoautotrophic metabolism of microalgae, the balanced-growth assumption appears to be unreasonable because of the synchronization of their circadian cycle on the daily light. Yet, understanding microalgae metabolism is necessary to optimize the production yield of bioprocesses based on this microorganism, as for example production of third-generation biofuels.In this PhD thesis, DRUM, a new dynamic metabolic modeling framework that handles the non-balanced growth condition and hence accumulation of intracellular metabolites was developed. The first stage of the approach consists in splitting the metabolic network into sub-networks describing reactions which are spatially and functionally close, and which are assumed to satisfy balanced growth condition. The left metabolites interconnecting the sub-networks behave dynamically. Then, thanks to Elementary Flux Mode analysis, each sub-network is reduced to macroscopic reactions, for which simple kinetics are assumed. Finally, an Ordinary Differential Equation system is obtained to describe substrate consumption, biomass production, products excretion and accumulation of some internal metabolites.DRUM was applied to the accumulation of lipids and carbohydrates of the microalgae Tisochrysis lutea under day/night cycles in normal and nitrogen starvation conditions. The resulting model describes accurately experimental data. It efficiently predicts the accumulation and consumption of lipids and carbohydrates. DRUM was also applied to the microalgae Chlorella Sorokiniana in dark heterotrophic growth, showing that the balanced-growth assumption was valid in this case
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Kaloudis, Dimitrios. "Improving microalgae for biofuel production." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.665443.

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Microalgae are a diverse group of oxygenic photosynthetic microorganisms which show great promise as a source of biofuel. However, significant challenges still remain before microalgae can be considered a viable source of biofuel. The main current challenges are nutrient sourcing and recycling as well as downstream processing. The algal cell wall and especially the presence of an algaenan cell wall in some Chlorophyte algae could be an important variable in determining downstream processing costs but not much comparative research has been done to elucidate this. The first part of the present study focuses on the recently isolated alga Pseudochoricystis ellipsoidea (Trebouxiophyceae) and its improvement and assessment for biofuel production. Random mutagenesis and FACS screening protocols were developed for the isolation of pigment and cell wall mutants but despite considerable efforts no suitable mutants could be identified in the first half of this project. Two 500 L raceway ponds as well as an algal growth room and bubble column bioreactors were set up to facilitate algal research at the University of Bath and assess the performance of P. ellipsoidea in realistic culture conditions. P. ellipsoidea showed a maximum growth of 1.53 divisions day-1 in semi-open raceway ponds, resistance to contamination and a 30% lipid content, making it particularly suitable for raceway pond cultures. In the second part of this project six species of Chlorophyte (“green”) algae, three of which produced algaenan, were compared for suitability to growth in anaerobic digestate and municipal wastewater as well as cell wall strength, permeability and suitability to hydrothermal liquefaction. We found that anaerobic digestate was a good medium for the growth of all species independently of autoclaving and that non-autoclaved wastewater was a very challenging medium. Algaenan production did not affect cell disruption by ultrasonication but growth stage and cell wall thickness did. Lipid extraction kinetics by chloroform/methanol were greatly affected by algaenan, meaning that this material is relatively impermeable to organic solvents. Cell wall thickness, cell volume and lipid content also had an effect on lipid extraction kinetics but this was only measurable after 180 minutes of extraction. 8 Hydrothermal liquefaction showed high solid and low oil yields, very low sulphur (≤0.1 %) as well as a 1.1 % -1.8 % nitrogen content which is significantly lower than most algal HTL studies to date. This suggests that stationary stage algae are more difficult to process but give a cleaner biocrude and reduce the loss of nitrogen through incorporation in the oil. Significant opportunities for optimisation still exist in the HTL process.
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Moulin, Solène. "Synthesis of hydrocarbons in algae : from biodiversity to biotechnology." Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0429.

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Les hydrocarbures (HCs) sont prédominants dans notre économie actuelle (carburants, cosmétiques, chimie, etc.) mais sont quasi-exclusivement issus des ressources fossiles. Les problématiques de changement climatique et d’épuisement des ressources poussent les recherches vers l’étude et la domestication des voies de synthèse naturelles d’HCs. Lorsque j’ai commencé ma thèse, une enzyme de biosynthèse d’HC, l’acide gras photodécarboxylase (FAP) venait d’être découverte chez la microalgue Chlorella. J’ai d’abord caractérisé son homologue chez la microalgue modèle Chlamydomonas. Une étude phylogénétique de la famille des GMC oxidoréductases à laquelle appartient la FAP a permis d’identifier un large réservoir de de 200 FAPs putatives. La caractérisation biochimique de plusieurs d’entre elles a permis de montrer qu’une FAP fonctionnelle a été conservée lors des endosymbioses secondaires. Cela suggère que la FAP joue un rôle important chez les algues. Ce rôle a été étudié par une approche de génétique inverse chez Chlamydomonas. La caractérisation physiologique de mutants knockout a permis de démontrer le rôle de la FAP dans la synthèse d’HCs dans le chloroplaste et de mettre en évidence des modifications physiologiques transitoires. Des mécanismes de compensation à l’absence d’HCs restent donc à découvrir. Dans une dernière partie, j’ai développé une souche d’E. coli exprimant la FAP et une thioestérase. Cette souche produit en continu des HCs dans la phase gaz des cultures, ce qui permet une récolte facilitée du produit d’intérêt sous forme pure. Cette étude constitue une preuve de concept que la FAP pourrait être utilisée pour la production biosourcée d’HCs
Hydrocarbons (HCs) are predominant in our current economy (fuels, cosmetics, chemicals, etc.) but are almost exclusively derived from fossil resources. Climate change and resource depletion concerns are pushing research towards the study and domestication of natural HC synthesis pathways. When I started my thesis, a HC forming enzyme, the fatty acid photodecarboxylase (FAP) had just been discovered in the microalgae Chlorella. I first characterised its homolog in the model microalgae Chlamydomonas. A phylogenetic study of the GMC oxidoreductase family to which the FAP belongs has allowed identification of a large reservoir of 200 putative FAPs. Biochemical characterisation of several of them showed that a functional FAP was maintained during secondary endosymbiosis. This suggests that FAP plays an important role in algae. This role has been studied by a reverse genetic approach in Chlamydomonas. The physiological characterisation of knockout mutants demonstrated the role of FAP in the synthesis of HCs in chloroplasts as well as transient physiological changes. Mechanisms to compensate for the absence of HCs therefore remain to be discovered. In a last part, I developed a strain of E. coli expressing the FAP and a thioesterase. This strain continuously produces HCs in the gas phase of the cultures, which allows an easier harvesting of the product of interest in a pure form. This study is a proof of concept that FAP could be used for the biobased production of HCs
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Sorigue, Damien. "Biosynthèse d'hydrocarbures dérivés des acides gras chez les microalgues." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4084.

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Les alcanes et les alcènes sont des hydrocarbures non cycliques important dans l’industrie. Ils sont synthétisés à partir d'acides gras par une grande variété d’organismes mais les connaissances à ce sujet sont très limitées chez les microalgues. Le but de ces travaux était donc de rechercher la présence d’alcanes ou d’alcènes dans diverses microalgues modèles, et d’essayer d’identifier la ou les enzymes responsables de la synthèse de ces composés. Nous avons mis en évidence la présence d’hydrocarbures linéaires en C15-C17 chez les microalgues Chlorella et Chlamydomonas. Ces composés étaient synthétisés uniquement en présence de lumière. L’absence dans le génome de ces microalgues d’homologues de gènes codant pour des enzymes connues de synthèse d’alcanes/alcènes a permis de conclure à la présence d’un nouveau système de synthèse d’hydrocarbures. Des purifications enzymatique et des analyses protéomique ont permis d’identifier une enzyme candidate qui exprimée chezE. coli est suffisante à la synthèse d’hydrocarbures. L'étude de cette enzyme révella qu'il s'agissait d'une photoenzyme utilisant l'énergie des photons bleue pour décarboxyler les acides grass en alca(e)ne. La structure de cette photoenzyme montre la présence un tunnel hydrophobe contenant l’acide gras et le cofacteur FAD. Cette nouvelle enzyme nommée « alcane photosynthase » amène de nombreuses question: qu'elle est la fonction des hydrocarbures chez ces microorganismes? Quel est le mécanisme catalytique de l’alcane photosynthase? Enfin, elle offre de nouvelles possibilités pour la production de biocarburants utilisant directement l’énergie solaire
Alkanes and alkenes are important in industry. Alkanes and alkenes are synthesized from fatty acids by a variety of organisms, such as plants and insects. However, the presence in microalgae of enzymes converting fatty acids into hydrocarbons has been poorly studied. The aim of this work was to investigate the presence of alkanes and alkenes in various microalgae models, and try to identify the enzymes responsible for the synthesis of these compounds.We have first demonstrated the presence of linear hydrocarbons C15-C17 in microalgae Chlorella and Chlamydomonas. Then we have shown that the main hydrocarbon formed in Chlorella and Chlamydomonas was derived from cis-vaccenic acid and was synthesized only in the presence of light. Absence of homologues of genes coding for known alkane/alkene biosynthetic enzymes in the genome of Chlorella and Chlamydomonas indicate the presence of an unknown pathway. Enzymatic purification and proteomic analysis allowed to identify a candidate enzyme which, expressed in E. coli lead to the formation of hydrocarbons with variable chain lengths, thus demonstrating that it was really an synthase alkane. Characterization showed that the enzyme was a photoenzyme, which used blue light to catalyse the decarboxylation of fatty acid to an alka(e)ne. The three-dimensional structure of this enzyme revealed a hydrophobic tunnel containing the fatty acid and the FAD cofactor
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King, P. M. "The use of ultrasound on the extraction of microalgal lipids." Thesis, Coventry University, 2014. http://curve.coventry.ac.uk/open/items/4aabbd22-686a-4284-a18d-23de6bcff203/1.

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Microalgae synthesize and store large volumes of lipids (potentially over 25% of dry weight) which could provide a renewable source of biodiesel. Traditional extraction techniques often produce poor lipid yields particularly from microalgae with robust cell walls. This project investigated the role of power ultrasound as a cell disruption step in lipid extraction from four microalgal species. Nile Red staining was used to assess the time when ultrasound induced increased membrane permeability in each species and lipids were extracted using an ultrasound assisted Bligh and Dyer extraction method. A 20 kHz probe system (40% amplitude, 0.086 W/cm3) caused increased lipid recovery from dry biomass in all cases; D. salina (no cell wall) from 15 to 22.5% of dry biomass after 1 minute (26% when stressed with 35 g/L NaCl). C. concordia (thin cell wall) from 7.5 to 10.5% of dry biomass after 2 minutes (27% with 25% nitrogen reduction in growth media). N. oculata (thick cell wall) from 6.5 to 10% of dry biomass after 16 minutes (31.5% when stressed with 30 g/L NaCl). The stressed cultures yield could be improved to 35% when ultrasound was combined with S070 beating beads. Chlorella sp. (thick cell wall) from 6.3 to 8.7% of dry biomass, after 16 minutes (44% was achieved when harvested at day 9 instead of 15). A Dual Frequency Reactor (16 and 20 kHz, 0.01 W/cm3) flow system with S070 beads demonstrated that high lipid extraction yields could be achieved on a larger level with N. oculata. After 4:48 minutes sonication 24% lipid recovery was achieved. This system could theoretically increase daily microalgal oil production from 3.96 to 5.76 L per day when compared to conventional techniques, at an extra production cost of only 2.9 p/litre (1.5% increase). D. salina, N. oculata and C. concordia resumed normal growth following sonication at 20 kHz after 1-20 days (8 minutes treatment for D. salina, 60 minutes treatment for N. oculata and 16 minutes treatment for C. concordia). It was found that the supernatant of sonicated D. salina and C. concordia when added to established cultures were able to boost their growth.
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Weiss, Annika Verfasser], Liselotte [Akademischer Betreuer] [Schebek, and Peter [Akademischer Betreuer] Cornel. "Energy balance of microalgae biofuels / Annika Weiss. Betreuer: Liselotte Schebek ; Peter Cornel." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2016. http://d-nb.info/1112332812/34.

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Lowrey, Joshua Bradley. "Seawater/Wastewater Production of Microalgae-based Biofuels in Closed Loop Tubular Photobioreactors." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/509.

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The push for alternatives to petroleum fuels has forced researchers to look for highly productive, renewable, non-food resources. The advantages of using microalgae instead of traditional oil crops for biofuel production include high oil yields, rapid reproductive rates, and versatile growing requirements. In order to reduce the cost of producing microalgae based biofuels, wastewater has been used as a nutrient source instead of specialized plant nutrients. The purpose of this study was to compare the relative effectiveness of different combinations of microalgae strain and dairy wastewater for increasing biomass. The methods for monitoring growth included optical density, cell counting, biomass estimation by chlorophyll-a, and volatile suspended solids. The analyses compared four concentrations of wastewater media as well as four strain treatments: Chlorella vulgaris, Tetraselmis sp., mixed freshwater culture and mixed saltwater culture. Optimum wastewater concentrations for microalgae growth were approximately 0% and 25% for most strain treatments. The results of the wastewater treatments concluded that dairy wastewater could serve as an effective nutrient substitute for plant food at concentrations approximately 25%. Chlorella vulgaris and Tetraselmis sp. prevailed over the mixed cultures for biomass production. Nitrate was the most limiting nutrient and exhibited the greatest reductions, sometimes in excess of 90%. The regression equations derived from the volatile suspended solids data achieved high R2 values and determined that total nitrogen, ammonium, and nitrate were significant in the model. In those equations, increasing either ammonium or nitrate yielded an increase in volatile suspended solids. With regards to comparing biomass quantification methods, the two most useful and reliable biomass quantification methods were optical density and volatile suspended solids.
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Weiss, Annika [Verfasser], Liselotte [Akademischer Betreuer] Schebek, and Peter [Akademischer Betreuer] Cornel. "Energy balance of microalgae biofuels / Annika Weiss. Betreuer: Liselotte Schebek ; Peter Cornel." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2016. http://nbn-resolving.de/urn:nbn:de:tuda-tuprints-53524.

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Books on the topic "Microalgae biofuels"

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Moheimani, Navid R., Mark P. McHenry, Karne de Boer, and Parisa A. Bahri, eds. Biomass and Biofuels from Microalgae. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16640-7.

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Gouveia, Luisa. Microalgae as a Feedstock for Biofuels. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17997-6.

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Alam, Md Asraful, and Zhongming Wang, eds. Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-2264-8.

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Microalgae Cultivation for Biofuels Production. Elsevier, 2020. http://dx.doi.org/10.1016/c2018-0-01358-4.

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Microalgae-Based Biofuels and Bioproducts. Elsevier, 2017. http://dx.doi.org/10.1016/c2015-0-05935-4.

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Gouveia, Luisa. Microalgae as a Feedstock for Biofuels. Springer, 2011.

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Microalgae As A Feedstock For Biofuels. Springer, 2011.

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Gouveia, Luisa. Microalgae as a Feedstock for Biofuels (SpringerBriefs in Microbiology). Springer, 2011.

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Algae for Biofuels and Energy (Developments in Applied Phycology). Springer, 2012.

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Moheimani, Navid R., Mark P. McHenry, Karne de Boer, and Parisa A. Bahri. Biomass and Biofuels from Microalgae: Advances in Engineering and Biology. Springer, 2015.

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Book chapters on the topic "Microalgae biofuels"

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Barbosa, Maria J., and René H. Wijffels. "Biofuels from Microalgae." In Handbook of Microalgal Culture, 566–77. Oxford, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118567166.ch29.

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Katiyar, Richa, Amit Kumar, and B. R. Gurjar. "Microalgae Based Biofuel: Challenges and Opportunities." In Biofuels, 157–75. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3791-7_9.

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Singh, Harshita, and Debabrata Das. "Biofuels from Microalgae: Biohydrogen." In Energy from Microalgae, 201–28. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_10.

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Bastos, Reinaldo Gaspar. "Biofuels from Microalgae: Bioethanol." In Energy from Microalgae, 229–46. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_11.

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Passos, Fabiana, Cesar Mota, Andrés Donoso-Bravo, Sergi Astals, David Jeison, and Raúl Muñoz. "Biofuels from Microalgae: Biomethane." In Energy from Microalgae, 247–70. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_12.

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Reijnders, Lucas. "Biofuels from Microalgae: Biodiesel." In Energy from Microalgae, 171–80. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_8.

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Sharma, P., M. B. Khetmalas, and G. D. Tandon. "Biofuels from Green Microalgae." In Biotechnology: Prospects and Applications, 95–112. New Delhi: Springer India, 2013. http://dx.doi.org/10.1007/978-81-322-1683-4_9.

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Peng, Kun, Jiashuo Li, Kailin Jiao, Xianhai Zeng, Lu Lin, Sharadwata Pan, and Michael K. Danquah. "The Bioeconomy of Microalgal Biofuels." In Energy from Microalgae, 157–69. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_7.

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Talebi, Ahmad Farhad, Meisam Tabatabaei, and Mortaza Aghbashlo. "Recent Patents on Biofuels from Microalgae." In Energy from Microalgae, 291–306. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69093-3_14.

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Bajpai, Pratima. "Production of Biofuel from Microalgae." In Third Generation Biofuels, 45–66. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2378-2_7.

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Conference papers on the topic "Microalgae biofuels"

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Yibin Tian, Chunhu Li, Junjie Bian, and Lijuan Feng. "Microalgae derived biofuels and processes." In Environment (ICMREE). IEEE, 2011. http://dx.doi.org/10.1109/icmree.2011.5930809.

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Sharma, Rohan, Scott Shirley, Tahir Farrukh, Mohammadhassan Kavosi, and Myeongsub Kim. "Microalgae Harvesting in a Microfluidic Centrifugal Separator for Enhanced Biofuel Production." In ASME 2020 18th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2020 Heat Transfer Summer Conference and the ASME 2020 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/icnmm2020-1078.

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Abstract Biofuel is one of the renewable energy resources alternatives to fossil fuels [1]. Among various sources for biofuels, microalgae provide at least three-orders-of-magnitude higher production rate of biodiesel at a given land area than conventional crop-based methods. However, microalgal biodiesel still suffers from significantly lower harvesting performance, making such a fuel less competitive. To increase the separation performance of microalgae from cultivation solution, we used a spiral microchannel that enables the isolation of biofuel-algae particles from water and contaminants contained in the culturing solution. Our preliminary data show that separation performance in the microfluidic centrifugal separator is as high as 88% within a quick separation time of 30 seconds. To optimize separation performance, multiple parameters of algae behaviors and separation techniques were studied and were manipulated to achieve better performance. We found that changing these factors altered the separation performance by increasing or decreasing flocculation, or “clumping” of the microalgae within the microchannels. The important characteristics of the separator geometry, fluid properties, and environmental conditions on algae separation was found and will be further studied in the forthcoming tests. This introductory study reveals that there is an opportunity to improve the currently low performance of algae separation in centrifugal systems using much smaller designs in size, ensuring a much more efficient algae harvesting.
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Melis, Tasios. "Optical Properties of Microalgae for Enhanced Biofuels Production." In Frontiers in Optics. Washington, D.C.: OSA, 2007. http://dx.doi.org/10.1364/fio.2007.smc4.

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Melis, Anastasios. "Optical Properties of Microalgae for Enhanced Biofuels Production." In Frontiers in Optics. Washington, D.C.: OSA, 2008. http://dx.doi.org/10.1364/fio.2008.jthb3.

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Rosa, Marcos P., Jose V. C. Vargas, Vanessa M. Kava, Fernando G. Dias, Daiani Savi, Beatriz Santos, Wellington Balmant, Andre B. Mariano, Andre Servienski, and Juan C. Ordóñez. "Hydrogen and Compounds With Biological Activity From Microalgae." In ASME 2019 13th International Conference on Energy Sustainability collocated with the ASME 2019 Heat Transfer Summer Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/es2019-3965.

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Abstract Microalgae have a high biotechnological potential as a source of biofuels (biodiesel, biohydrogen) and other high-added value products (e.g., pharmaceuticals, proteins, pigments). However, for microalgae cultivation to be economically competitive with other fuel sources, it is necessary to apply the concept of biorefinery. This seems to be the most ambitious strategy to achieve viability. Therefore, the objectives of this study were to isolate and identify the main microalgae line used to produce biofuels at Federal University of Parana, Brazil, using the rDNA sequence and micromorphological analysis, and to evaluate the potential of this lineage in the production of hydrogen and co-products with biological activity. For the purification of the lineage (LGMM0001), an aliquot was seeded into solid CHU culture medium and an isolated colony was selected. The genomic DNA was purified using a commercial kit (Macherey-Nagel, Düren, Germany) for molecular identification, the ITS region (ITS1, 5.8S and ITS2) (Internal Transcribed Spacer) was amplified and sequenced using primers LS266 and V9G. Morphological characterization was performed as described by Hemschemeier et al. [1]. Finally, for biological activity research, secondary metabolites were extracted by fractionation and evaluated against bacteria of clinical interest. Through microscopic analysis, general characteristics shared by the genus Tetradesmus were observed. The plasticity of the morphological characteristics of this genus reinforces the need for further studies to classify correctly the species in this group, using DNA sequencing. ITS sequence analysis of LGMM0001 showed 100% homology with sequences from the Tetradesmus obliquus species, so, the lineage was classified as belonging to this species. The evaluated microalgae strain was able to produce hydrogen, showing positive results for gas formation. Biological activity was observed with the extract obtained from the residual culture carried out with alternative medium used in the photobioreactors (PBR), against the Staphylococcus aureus pathogenic lineage. In conclusion, the microalgae strain used in this work was identified as Tetradesmus obliquus (= Acutodesmus obliquus), and was able to produce a compound with economic potential in association with the existing biofuel production process.
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Madugu, F., and M. Collu. "Techno-economic modelling analysis of microalgae cultivation for biofuels and co-products." In ENERGY QUEST 2014. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/eq141022.

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Cui, Yan, Wenqiao Wayne Yuan, and Zhijian Pei. "Effects of Carrier Material and Design on Microalgae Attachment for Biofuel Manufacturing: A Literature Review." In ASME 2010 International Manufacturing Science and Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/msec2010-34150.

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Continuous use of petroleum derived fuels is widely recognized as unsustainable due to depleting supplies and the accumulation of greenhouse gases in the environment. Renewable, carbon neutral transport fuels are needed for environmental and economic sustainabilities. Algae have been demonstrated to be one of the most promising sources for biofuel production. However, large-scale algae production and harvesting for energy manufacturing are too costly using existing methods. The approach of growing algae on solid carriers is innovative and can potentially lead to cost-effective manufacturing of algae biofuels. As cells approach to the solid surface, many factors come in to influence microbial attachment such as the surface wettability, free energy, polarity, roughness and topography. Surface wettability plays an important role in the initial cell attachment. For further contact, surface free energy and polarity are more directly related to cell-substratum attachment strength. Surface roughness and texture are species-specific parameters and have been applied widely in attachment studies.
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Van Den Bos, Patricia, Judith Jahn, and Leo J. Van Den Broeke. "Sustainable Production of Biochemicals and Biofuels Based on Biofixation of Carbon Dioxide by Microalgae." In SPE International Production and Operations Conference & Exhibition. Society of Petroleum Engineers, 2012. http://dx.doi.org/10.2118/157396-ms.

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Bucy, Harrison, and Anthony J. Marchese. "Oxidative Stability of Algae Derived Methyl Esters Containing Varying Levels of Methyl Eicosapentaenoate and Methyl Docosahexaenoate." In ASME 2011 Internal Combustion Engine Division Fall Technical Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/icef2011-60047.

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Microalgae is currently receiving strong consideration as a potential biofuel feedstock to help meet the advanced biofuels mandate of the 2007 Energy Independence and Security Act because of its theoretically high yield (gallons/acre/year) in comparison to current terrestrial feedstocks. Microalgae lipids can be converted into a variety of biofuels including fatty acid methyl esters (e.g. biodiesel), renewable diesel, renewable gasoline or synthetic paraffinic aviation kerosene. For algal methyl ester biodiesel, fuel properties will be directly related to the fatty acid composition of the lipids produced by the given microalgae strain. Several microalgae species under consideration for wide scale cultivation, such as Nannochloropsis, produce lipids with fatty acid compositions containing substantially higher quantities of long chain-polyunsaturated fatty acids (LC-PUFA) in comparison to terrestrial feedstocks. It is expected that increased levels of LC-PUFA will be problematic in terms of meeting all of the current ASTM specifications for biodiesel. For example, it is well known that oxidative stability decreases with increasing levels of LC-PUFA. However, these same LC-PUFA fatty acids, such as eicosapentaenoic acid (EPA: C20:5) and docosahexaenoic acid (DHA: C22:6) are known to have high nutritional value thereby making separation of these compounds economically attractive. Given the uncertainty in the future value of these LC-PUFA compounds and the economic viability of the separation process, the goal of this study was to examine the oxidative stability of algal methyl esters with varying levels of EPA and DHA. Tests were conducted using a Metrohm 743 Rancimat with automatic induction period determination following ASTM D6751 and EN 14214 standards, which call for induction periods of at least 3 hours and 6 hours, respectively. Tests were conducted at a temperature of 110°C and airflow of 10 L/h with model algal methyl ester compounds synthesized from various sources to match the fatty acid compositions of several algae strains subjected to varying removal amounts of roughly 0 to 100 percent LC-PUFA. In addition, tests were also conducted with real algal methyl esters produced from multiple sources. The bis-allylic position equivalent (BAPE) was calculated for each fuel sample to quantify the level of unsaturation. The induction period was then plotted as a function of BAPE, which showed that the oxidative stability varied exponentially with the amount of LC-PUFA. The results suggest that removal of 45 to 65 percent of the LC-PUFA from Nannochloropsis-based algal methyl esters would be sufficient for meeting existing ASTM specifications for oxidative stability.
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ROKICKA, Magdalena, Marcin ZIELIŃSKI, and Marcin DĘBOWSKI. "LIPIDS ACCUMULATION OF CHLORELLA VULGARIS UNDER VARIABLE LIGHTING CONDITIONS." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.203.

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The cultivation of microalgae is now an intensively developed research area. Some species of microalgae under appropriate conditions accumulate large amounts of lipids in the cells, which may be a suitable feedstock for biodiesel production. The cultures of microalgae for lipids production should be cultivated in specific physicochemical conditions. The most important environmental parameters affecting the algae growth are: nutrients, lighting, reaction, turbulence, salinity and temperature. Periodic changes in lighting is a key parameter that have a significant effect on cells density and lipid accumulation. The mechanism of this action depends on intensity of light and its spectral composition. To produce 3rd and 4th generation biofuels, a better understanding of the relationship between light conditions and yield of lipids accumulation is necessary. The aim of the study was to determine the effects of variable lighting conditions for lipids accumulation of microalgae Chlorella vulgaris and to determine the most effective lighting parameters. The study confirmed the possibility of using the lighting shock conditions to maximize lipids accumulation in algae Chlorella vulgaris cells. In the study, 33.18% of lipids were obtained from biomass culturing with red light-emitting diodes (LEDs), which was 22% more than obtained with white continuous lighting.
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Reports on the topic "Microalgae biofuels"

1

Polle, J. Creating a Collection of Microalgae for use in Biofuels Research. Fort Belvoir, VA: Defense Technical Information Center, June 2008. http://dx.doi.org/10.21236/ada484623.

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Cooksey, Keith E., Matthew Fields, Brent Peyton, and Ross Carlson. Lipid-Derived Biofuels: Determination of Factors that Control Triglyceride Accumulation in Microalgae. Fort Belvoir, VA: Defense Technical Information Center, October 2012. http://dx.doi.org/10.21236/ada581861.

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Zhu, Yunhua, Susanne Jones, Andrew Schmidt, Heather Job, Justin Billing, James Collett, Kyle Pomraning, et al. Microalgae Conversion to Biofuels and Biochemical via Sequential Hydrothermal Liquefaction (SEQHTL) and Bioprocessing: 2020 State of Technology. Office of Scientific and Technical Information (OSTI), April 2021. http://dx.doi.org/10.2172/1784347.

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Sayre, Richard. Optimization of Biofuel Production from Transgenic Microalgae. Fort Belvoir, VA: Defense Technical Information Center, May 2008. http://dx.doi.org/10.21236/ada586572.

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Colucci, Jose, Govind Nadathur, Vilmaris Bracero, William Rosado, Miriam Fontalvo, Jesus Garcia, Cecilia Diaz, Luis Colon, Adrian Lopez, and Giovanna Santiago. Propulsion and Power Rapid Response Research and Development (R&D) Support. Task Order 0004: Advanced Propulsion Fuels R&D, Subtask: Optimization of Lipid Production and Processing of Microalgae for the Development of Biofuels. Fort Belvoir, VA: Defense Technical Information Center, February 2013. http://dx.doi.org/10.21236/ada582355.

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Umbach, Brynn E. Characterization of Microalgal Lipids for Optimization of Biofuels. Fort Belvoir, VA: Defense Technical Information Center, May 2014. http://dx.doi.org/10.21236/ada604782.

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Pienkos, Philip. Isolation, Preliminary Characterization and Preliminary Assessment of Scale-Up Potential of Photosynthetic Microalgae for the Production of Both Biofuels and Bio-Active Molecules in the U.S. and Canada: Cooperative Research and Development Final Report, CRADA Number CRD-10-372. Office of Scientific and Technical Information (OSTI), September 2012. http://dx.doi.org/10.2172/1051892.

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Ly, A., K. A. Rolison, X. Mayali, and T. J. Samo. DE-STRESSING BIOFUEL MICROALGAE: LEVERAGING BENEFICIAL MICROBIOMES TO ALLEVIATE HARMFUL CONDITIONS THAT REDUCE ALGAL GROWTH. Office of Scientific and Technical Information (OSTI), August 2019. http://dx.doi.org/10.2172/1557936.

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Hildebrand, Mark, Juergen Polle, and Michael Huesemann. A Systems Biology and Pond Culture-based Understanding and Improvement of Metabolic Processes Related to Productivity in Diverse Microalgal Classes for Viable Biofuel Production. Office of Scientific and Technical Information (OSTI), July 2018. http://dx.doi.org/10.2172/1458513.

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