Academic literature on the topic 'Microalgae metabolism'

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Journal articles on the topic "Microalgae metabolism"

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Gonzalez-Bashan, Luz E., Vladimir K. Lebsky, Juan P. Hernandez, Jose J. Bustillos, and Yoav Bashan. "Changes in the metabolism of the microalga Chlorella vulgaris when coimmobilized in alginate with the nitrogen-fixing Phyllobacterium myrsinacearum." Canadian Journal of Microbiology 46, no. 7 (2000): 653–59. http://dx.doi.org/10.1139/w00-041.

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In an agroindustrial wastewater pond, a naturally occurring unicellular microalga, Chlorella vulgaris, was closely associated with the terrestrial plant-associative N2-fixing bacterium Phyllobacterium myrsinacearum. When the two microorganisms were artificially coimmobilized in alginate beads, they shared the same internal bead cavities, and the production of five microalgal pigments increased, but there were no effects on the number of the cells or the biomass of the microalga. The association, however, reduces the ability of C. vulgaris to remove ammonium ions and phosphorus from water. The
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Bellido-Pedraza, Carmen M., Maria J. Torres, and Angel Llamas. "The Microalgae Chlamydomonas for Bioremediation and Bioproduct Production." Cells 13, no. 13 (2024): 1137. http://dx.doi.org/10.3390/cells13131137.

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The extensive metabolic diversity of microalgae, coupled with their rapid growth rates and cost-effective production, position these organisms as highly promising resources for a wide range of biotechnological applications. These characteristics allow microalgae to address crucial needs in the agricultural, medical, and industrial sectors. Microalgae are proving to be valuable in various fields, including the remediation of diverse wastewater types, the production of biofuels and biofertilizers, and the extraction of various products from their biomass. For decades, the microalga Chlamydomonas
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González-González, Lina Maria, and Luz E. de-Bashan. "Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia." Biology 10, no. 4 (2021): 282. http://dx.doi.org/10.3390/biology10040282.

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Engineered mutualistic consortia of microalgae and bacteria may be a means of assembling a novel combination of metabolic capabilities with potential biotechnological advantages. Microalgae are promising organisms for the sustainable production of metabolites of commercial interest, such as lipids, carbohydrates, pigments, and proteins. Several studies reveal that microalgae growth and cellular storage of these metabolites can be enhanced significantly by co-cultivation with growth-promoting bacteria. This review summarizes the state of the art of microalgae–bacteria consortia for the producti
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de-Bashan, Luz E., Yoav Bashan, Manuel Moreno, Vladimir K. Lebsky, and Jose J. Bustillos. "Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense." Canadian Journal of Microbiology 48, no. 6 (2002): 514–21. http://dx.doi.org/10.1139/w02-051.

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Three strains of the freshwater microalgae used for wastewater treatment, Chlorella vulgaris and Chlorella sorokiniana co-immobilized separately in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense Cd, resulted in significant changes in microalgal-population size, cell size, cell cytology, pigment, lipid content, and the variety of fatty acids produced in comparison with microalgae immobilized in alginate without the bacterium. Cells of C. vulgaris UTEX 2714 did not change in size, but the population size within the beads significantly increased. On the othe
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Amir, Azhar, Tanda S. S. Panjaitan, Yudi Adinata, et al. "Microalgae Application in Ruminants Feeds on The Production and Quality of Meat and Milk: A Review." IOP Conference Series: Earth and Environmental Science 1360, no. 1 (2024): 012011. http://dx.doi.org/10.1088/1755-1315/1360/1/012011.

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Abstract Research focusing on microalgal as an alternative source of protein and fat in ruminant feed shows a growing trend. Microalgae are rich in carbohydrates, proteins, fats, amino acids, vitamins, minerals, and carotene. Ruminants can digest the cell walls of microalgae, positively affecting their health, growth, metabolism, reproduction, production, and product quality. This paper introduces the latest review of microalgae on ruminant feed and its effect on performance, production, and quality of meat and milk. The results showed that using microalgae does not increase milk production in
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Garofalo, Cristiana, Alessandra Norici, Lorenzo Mollo, Andrea Osimani, and Lucia Aquilanti. "Fermentation of Microalgal Biomass for Innovative Food Production." Microorganisms 10, no. 10 (2022): 2069. http://dx.doi.org/10.3390/microorganisms10102069.

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Fermentation is an ancient method used worldwide to process and preserve food while enhancing its nutraceutical profile. Alga-based fermented products have recently been developed and tested due to growing interest in healthy sustainable diets, which demands the development of innovative practices in food production, operating for both human health and Earth sustainability. Algae, particularly microalgae such as Arthrospira platensis, Chlorella vulgaris, and Dunaliella salina, are already cultivated as sources of food due to their valuable compounds, including proteins, pigments, lipids, carot
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Moreira, Juliana Botelho, Bruna da Silva Vaz, Bruna Barcelos Cardias, et al. "Microalgae Polysaccharides: An Alternative Source for Food Production and Sustainable Agriculture." Polysaccharides 3, no. 2 (2022): 441–57. http://dx.doi.org/10.3390/polysaccharides3020027.

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Carbohydrates or polysaccharides are the main products derived from photosynthesis and carbon fixation in the Calvin cycle. Compared to other sources, polysaccharides derived from microalgae are safe, biocompatible, biodegradable, stable, and versatile. These polymeric macromolecules present complex biochemical structures according to each microalgal species. In addition, they exhibit emulsifying properties and biological characteristics that include antioxidant, anti-inflammatory, antitumor, and antimicrobial activities. Some microalgal species have a naturally high concentration of carbohydr
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Bossa, Rosanna, Melania Di Colandrea, Giovanna Salbitani, and Simona Carfagna. "Phosphorous Utilization in Microalgae: Physiological Aspects and Applied Implications." Plants 13, no. 15 (2024): 2127. http://dx.doi.org/10.3390/plants13152127.

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Phosphorus (P) is a fundamental element for life, playing an integral role in cellular metabolism including energy transfer, nucleic acid synthesis, and membrane structure. This nutrient is critical to the physiological ecology in all photosynthetic organisms including eukaryotic microalgae and cyanobacteria. The review, here presented, delves into the intricate mechanisms governing phosphorus acquisition from the environment, its utilization in plant metabolism, and regulation in these photosynthetic microorganisms. Furthermore, it comprehensively explores the strategies employed by microalga
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Saini, Dinesh Kumar, Geetanjali Manchanda, Ratiram Gomaji Chaudhary, and Raghvendra Pratap Singh. "Microalgal Metabolomes and Recent Biotechnological Advances for Their Industrial Application." Microbiology Research 15, no. 4 (2024): 2056–69. http://dx.doi.org/10.3390/microbiolres15040138.

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In recent decades, microalgae have emerged as new sustainable resources for the production of various bioactive and biochemical compounds. These organisms are photosynthetic, use CO2 as their sole carbon source, and grow rapidly under different environmental conditions. This makes microalgae a promising source of feedstock for many bio-commodities. However, the economic viability for large-scale production through microalgal cells is limited to certain organisms. Recent advances in omics technologies, along with high-throughput approaches, have provided new insights for understanding microalga
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Siti Zulaiha. "Genetic engineering of microalgae lipid biosynthesis for sustainable biodiesel production." World Journal of Advanced Research and Reviews 11, no. 3 (2021): 072–77. http://dx.doi.org/10.30574/wjarr.2021.11.3.0397.

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Biofuel is one of the most promising alternative energy sources for reducing human reliance on fossil fuels. Microalgae has recently emerged as the most promising biofuel source. However, biofuels from microalgae are still not feasible to replace fossil fuels because of their high production costs, therefore, it is necessary to pick microalgae species with high growth rates and lipid content. Overexpression of lipid biosynthesis enzymes and inhibition of competitive metabolic pathways are two genetic engineering strategies that can be developed to assess microalgae lipid production. Malate and
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Dissertations / Theses on the topic "Microalgae metabolism"

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Prioretti, Laura. "Sulfur metabolism in microalgae." Doctoral thesis, Università Politecnica delle Marche, 2015. http://hdl.handle.net/11566/243011.

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La concentrazione media di SO4 2- in oceano è 28-30 mmol L-1. Essa ha subito notevoli variazioni nel corso della storia della Terra e potrebbe aver facilitato la radiazione delle alghe della linea rossa (alghe con chl a+c quali diatomee, dinoflagellate e coccolitoforidi) a discapito di quelle della linea verde (alghe con chl a+b) e dei cianobatteri. È ancora da chiarire se gli enzimi responsabili dell’assimilazione del SO4 2- negli organismi fotosintetici abbiano svolto un ruolo nel controllare l’adattamento delle alghe alle variazioni di concentrazione del SO4 2-. In accordo con stud
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LIN, HONGMIN. "Sulfur Metabolism in Microalgae." Doctoral thesis, Università Politecnica delle Marche, 2018. http://hdl.handle.net/11566/259778.

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Gli organismi fotosintetici acquisiscono lo zolfo in forma di solfato e lo assimilano, in un processo riduttivo, come solfuro. L’amminoacido cisteina è il primo composto organico in cui lo zolfo viene incorporato al termine della via di assimilazione. Perché il solfato possa essere ridotto, deve prima essere attivato ad AdenosinFosfosolfato (APS). Questa reazione è catalizzata dall’enzima ATP-solforilasi, che nella gran parte delle alghe, eccetto dinoflagellate e -cianobatteri, e a differenza di tutti gli altri organismi, è soggetta a regolazione redox. Gli altri passaggi della via di assim
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Abida, Heni. "Characterization of lipid metabolism in the marine diatom Phaeodactylum tricornutum." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS231.

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L’océan domine la surface de notre planète et joue un rôle majeur dans la régulation de notre biosphère. Par exemple, les microorganismes photosynthétiques vivant dans l’océan produisent 50% de l’oxygène que nous respirons tous les ans, et une grande partie de notre alimentation et des ressources minérales en proviennent. En cette époque de crise écologique liée à l’accumulation anthropogénique de gaz à effet de serre dans l’atmosphère, il est impératif de développer des énergies plus durables que les carburants fossiles. Le biodiesel pourrait être une source de carburant viable et durable pou
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POUSA, KURPAN NOGUEIRA DANIEL. "Diversity of sulfur metabolism in microalgae." Doctoral thesis, Università Politecnica delle Marche, 2021. http://hdl.handle.net/11566/289649.

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Lo zolfo è un macronutriente fondamentale, ma spesso sottovalutato sostanzialmente meno informazioni su di esso in letteratura rispetto ad altri macronutrienti. Tra gli organismi fotosintetici, il metabolismo dello zolfo è generalmente si presume di essere conservato e la maggior parte delle informazioni disponibili è limitata alle pianti vascolari. L'indagine sulle alghe è limitata a pochissimi cladi, nonostante il importanza del solfato - la forma più disponibile di zolfo in natura - nell'acqua di mare. Infatti, ci sono prove che suggeriscono che la concentrazione di solfato nell'acqua di ma
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Lippmeier, James Casey. "Fatty acid metabolism of marine microalgae." Thesis, University of Hull, 2007. http://hydra.hull.ac.uk/resources/hull:7014.

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Pathways for the biosynthesis of docosahexaenoic acid (DHA) and other polyunsaturated fatty acids (PUFA) were elucidated in two heterotrophic, marine microalgae; Schizochytrium sp. and Crypthecodinium cohnii. PUFA-requiring auxotrophs of both of these algae were created and used as tools for studying PUFA biosynthetic pathways. Additionally, equilibrium radio-labeling techniques were applied to algal cultures fed 14C-fatty acids. Both organisms were found to possess two distinct pathways for PUFA biosynthesis. One pathway, mediated by classical elongases and desaturases, was incomplete in both
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Bouillaud, Dylan. "Multiscale NMR analysis of the microalgae metabolism." Thesis, Nantes, 2020. http://www.theses.fr/2020NANT4029.

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Les bio-procédés à base de microalgues sont reconnus pour des applications majeures comme la production de lipides précurseurs de biocarburant. Aujourd'hui la plupart de ces technologies vertes ne sont pas suffisamment matures en raison de coûts de production importants et sont pour cela activement développées par une importante communauté de bio-procédés. A l'interface entre la biologie et les procédés, cette communauté n'est pas familière avec la spectroscopie RMN, outil puissant d'analyse structurale et quantitative. L'objectif de cette thèse est d’évaluer le potentiel d’approches RMN multi
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Siripornadulsil, Surasak. "Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii) /." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486462702465653.

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Baroukh, Caroline. "Metabolic modelling under non-balanced growth : application to microalgae growth for biofuels production." Thesis, Montpellier 2, 2014. http://www.theses.fr/2014MON20190.

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La modélisation métabolique est un outil performant pour mieux comprendre, prédire et optimiser les bioprocédés, particulièrement lorsqu'ils impliquent des molécules d'intérêt. Malheureusement, l'utilisation de cette approche de modélisation pour des métabolismes dynamiques est difficile à cause du manque de données expérimentales nécessaires pour définir et calibrer les cinétiques des réactions appartenant aux chemins métaboliques. C'est pourquoi, les modèles métaboliques sont souvent utilisés sous l'hypothèse de croissance équilibrée. Cependant, pour certains procédés comme la croissance pho
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Cagnon, Caroline. "Une approche de génétique classique pour l' isolement et la caractérisation de mutants affectés dans la remobilisation des lipides chez Chlamydomonas reinhardtii." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4012.

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Les microalgues accumulent de grandes quantités d’huile, et sont de bons candidats pour la production de biocarburants. Mais des verrous techniques et biologiques doivent être levés pour une production rentable. Augmenter la teneur en huile par cellule et découvrir des protéine clés du métabolisme des triglycérides sont des objectifs importants. Nous avons mis en place une approche de génétique classique ciblée sur l’isolement de mutants d’insertion affectés dans la remobilisation des lipides de réserves suite à la re-supplémentation en azote après carence. Nous avons mis au point un protocole
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Gonzalez, Luz Estela de Bashan. "Ammonium metabolism coupled with indole-3-acetic acid in the microalgae Chlorella vulgaris when co-immobilized in alginate beads with the microalgae growth-promoting bacterium Azospirillum brasilense." Thesis, Université Laval, 2006. http://www.theses.ulaval.ca/2006/23707/23707.pdf.

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Books on the topic "Microalgae metabolism"

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Laliberté, Gilles *. Proline and arginine metabolism in green microalgae in relation to osmoregulation and heterotrophy. 1990.

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Yao, Changhong, Wei Xiong, and Chengwei Liang, eds. Microalgae for Metabolite Production Under Stress Conditions. Frontiers Media SA, 2022. http://dx.doi.org/10.3389/978-2-88974-401-5.

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Book chapters on the topic "Microalgae metabolism"

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Khozin-Goldberg, Inna. "Lipid Metabolism in Microalgae." In The Physiology of Microalgae. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-24945-2_18.

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Tsygankov, Anatoly, and Azat Abdullatypov. "Hydrogen Metabolism in Microalgae." In Photosynthesis. John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781119084150.ch3.

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Giordano, Mario, and Laura Prioretti. "Sulphur and Algae: Metabolism, Ecology and Evolution." In The Physiology of Microalgae. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-24945-2_9.

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Miyachi, S., K. Kuchitsu, and Y. Yagawa. "Responses of Microalgae to Changes in CO2 Concentration." In Inorganic Nitrogen Metabolism. Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71890-8_2.

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Kumar, Arun, and Jay Shankar Singh. "Microalgae II: Cell Structure, Nutrition and Metabolism." In Microalgae in Waste Water Remediation. CRC Press, 2021. http://dx.doi.org/10.1201/9780429298080-2.

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Glibert, Patricia M. "Silica Uptake, Assimilation, and Metabolism: Making Cell Walls." In Phytoplankton Whispering: An Introduction to the Physiology and Ecology of Microalgae. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-53897-1_14.

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Ogbonna, James Chukwuma, and Navid R. Moheimani. "Potentials of Exploiting Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae." In Biofuel and Biorefinery Technologies. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16640-7_3.

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Ogbonna, James Chukwuma, and Mark P. McHenry. "Culture Systems Incorporating Heterotrophic Metabolism for Biodiesel Oil Production by Microalgae." In Biofuel and Biorefinery Technologies. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16640-7_4.

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Glibert, Patricia M. "Phosphorus Uptake, Assimilation, and Metabolism: Making Energy and Genetic Material." In Phytoplankton Whispering: An Introduction to the Physiology and Ecology of Microalgae. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-53897-1_13.

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Glibert, Patricia M. "Nitrogen Uptake, Assimilation, and Metabolism: Making Amino Acids and Protein." In Phytoplankton Whispering: An Introduction to the Physiology and Ecology of Microalgae. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-53897-1_12.

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Conference papers on the topic "Microalgae metabolism"

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Natsi, P. D., and P. G. Koutsoukos. "Calcium Carbonate Biofouling in the Presence of Heavy Metals." In CORROSION 2021. AMPP, 2021. https://doi.org/10.5006/c2021-16606.

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ABSTRACT The presence of heavy metals in wastewater is significant to the extent they are involved in biofouling. Microalgae and other microorganisms play a significant role in the transport of heavy metal ions in the environment while at the same time they are closely related to the formation of minerals in aquatic systems, mainly with the formation of calcium carbonate. Metabolic byproducts of microalgae act as sequestrants of metal ions, thus contributing to the transport of heavy metals on mineral deposits. In this study, the effect of zinc and cadmium in the precipitation of calcium carbo
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Divi, Suresh. "Case Studies of Microbiological Influenced Corrosion (MIC) in Pipelines." In CONFERENCE 2025. AMPP, 2025. https://doi.org/10.5006/c2025-00168.

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Abstract Microbiologically influenced corrosion (MIC), also known as microbial corrosion, is a type of electrochemical corrosion that can be caused by the activity of microorganisms in oil and gas pipeline. Pipeline corrosion is influenced by a large number of factors such as fluid type, fluid chemistry, temperature, pH, aeration, and microorganisms. MIC is the one closely related to the activity of living microorganisms which includes microalgae, bacteria, archaea, and fungi. MIC, in any form, starts with the formation of a biofilm on the metal substrate and produces metabolic products which
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Malcata, F. Xavier. "Engineering of microalgae toward biodiesel: Facts and prospects." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/jeul5047.

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Excessive release to the atmosphere of greenhouse-effect gases—arising from combustion of fossil fuels, has urged a worldwide search for alternative sources of environment-friendly fuels; microalgae constitute an interesting possibility, owing to their widespread presence in most habitats and unique ability to synthesize oil. Microalgae require indeed only sunlight and water to grow—both freely available; together with CO2 as source of carbon—which concomitantly conveys a path for its direct sequestering from the atmosphere; and low-cost inorganic sources of phosphorus and nitrogen. However, t
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Sardar, Rahila, Kashif M. Shaikh, and Pavan P. Jutur. "Identification of transcription hubs that control lipid metabolism and carbon concentrating mechanism in model microalgae chlamydomonas reinhardtii using regulatory networks: Regulatory networks hubs in C. reinhardtii that control lipid and carbon concentrating metabolic pathways." In 2016 International Conference on Bioinformatics and Systems Biology (BSB). IEEE, 2016. http://dx.doi.org/10.1109/bsb.2016.7552116.

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Pessoa, Jassiara da Silva, Caroline Frere Martiniuc De Oliveira, Jesús Pascual Mena-Chalco, João Carlos Monteiro De Carvalho, and Livia Seno Ferreira Camargo. "VIAS METABÓLICAS E A SÍNTESE DE LIPÍDEOS NA MICROALGA CHLAMYDOMONAS REINHARDTII: UM ESTUDO CIENCIOMÉTRICO." In I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1385.

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Introdução: O uso de microalga para produção de biodiesel é uma alternativa sustentável frente a crescente demanda mundial de combustível. A Chlamydomonas reinhardtii é considerada uma microalga modelo, além de ser promissora para produção de biodiesel. Estratégias para aprimoramento do cultivo são necessárias para que essa plataforma se torne mais vantajosa economicamente. O crescimento microalgal em diferentes vias metabólicas tem impacto no crescimento e síntese de compostos intracelulares, como os lipídeos. Tais metabolismos se diferem pela forma em que as células obtêm energia, e são tipi
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Dalgamouni, Tasneem atef, Shatha Kanji, Maroua Cherif, et al. "Isolation, Cultivation, and Characterization of Novel Local Marine Micro-Algae for Aquaculture Feed Supplement Production." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0037.

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Aquaculture is considered as a promising alternative to support the food demands of the everincreasing population. Currently, this sector faces several challenges such as using fishmeal, which is unsustainable and expensive. Therefore, it is necessary to identify an alternative feed component that is sustainable, cost-effective and can provide the essential nutrients required by the fish. In this context, microalgae are considered as a viable source of proteins, lipids, polysaccharides and highvalue products (HVPs) such as essential fatty acids, amino acids and vitamins. They play a vital role
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Glazunova, Darina, Liliya Biktasheva, Polina Kuryntseva, and Polina Galitskaya. "CARBON SEQUESTRATION FROM INDUSTRIAL EMISSIONS USING MICROALGAE: RESULTS OF LABORATORY MODELING." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/4.1/s19.38.

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Global climate change is one of the most acute environmental problems of our time. Among the methods of capturing CO2 in industrial emissions are chemical, physical and biological. The latter are based on the use of living organisms that absorb carbon dioxide in metabolic processes, and the subsequent use or burial of their biomass or their metabolic products. One variant of organisms used to capture carbon dioxide biotechnologically are microalgae. In the present study, the efficiency to capture carbon from the exhaust gas was compared for three microalgae species - Chlorella vulgaris, Scened
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Jeon, Minkyu, Boeun Kim, Mingyu Sung, and Jay H. Lee. "On integrating the Droop model with the flux balance model for predicting metabolic shifts in microalgae growth." In 2014 14th International Conference on Control, Automation and Systems (ICCAS). IEEE, 2014. http://dx.doi.org/10.1109/iccas.2014.6987810.

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Dugo, Paola, Francesca Rigano, and Luigi Mondello. "Lipidomic analysis in food: The role of a detailed elucidation of intact lipids in functional foods for investigating on nutritional aspects." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/chnd8051.

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The growing demand in natural matrices, representing a source of dietary and nutraceutical molecules, placed, as direct consequence, the urgent need for the development of suitable analytical methods able to provide a comprehensive characterization of both “conventional” and “unconventional” products. In the last decades, lipidomic has emerged as a cutting-edge approach among omics- techniques, since lipids revealed to be essential molecules in the regulation of metabolic pathways. To this regard, the content of essential fatty acids (EFAs), as well as nutritional indices such as the levels of
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Reports on the topic "Microalgae metabolism"

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Hildebrand, Mark, Juergen Polle, and Michael Huesemann. A Systems Biology and Pond Culture-based Understanding and Improvement of Metabolic Processes Related to Productivity in Diverse Microalgal Classes for Viable Biofuel Production. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1458513.

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