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Journal articles on the topic 'Microalgal culture'

Author: Grafiati

Published: 10 March 2023

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1

Gonzalez, Luz E., and Yoav Bashan. "Increased Growth of the Microalga Chlorella vulgariswhen Coimmobilized and Cocultured in Alginate Beads with the Plant-Growth-Promoting Bacterium Azospirillum brasilense." Applied and Environmental Microbiology 66, no. 4 (2000): 1527–31. http://dx.doi.org/10.1128/aem.66.4.1527-1531.2000.

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ABSTRACT Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacteriumAzospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigments significantly increased. Light microscopy revealed that both microorganisms colonized the same cavities inside the beads, though the microalgae tended to concentrate in the more aerated periphe

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2

Wang, Hui, Haywood D. Laughinghouse, Matthew A. Anderson, et al. "Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1." Applied and Environmental Microbiology 78, no. 5 (2011): 1445–53. http://dx.doi.org/10.1128/aem.06474-11.

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ABSTRACTIncreasing petroleum costs and climate change have resulted in microalgae receiving attention as potential biofuel producers. Little information is available on the diversity and functions of bacterial communities associated with biofuel-producing algae. A potential biofuel-producing microalgal strain,Nannochloropsis oceanicaIMET1, was grown in Permian groundwater. Changes in the bacterial community structure at three temperatures were monitored by two culture-independent methods, and culturable bacteria were characterized. After 9 days of incubation,N. oceanicaIMET1 began to aggregate

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3

Yu, Hyeonjung, Jaai Kim, Chaeyoung Rhee, Juhee Shin, Seung Gu Shin, and Changsoo Lee. "Effects of Different pH Control Strategies on Microalgae Cultivation and Nutrient Removal from Anaerobic Digestion Effluent." Microorganisms 10, no. 2 (2022): 357. http://dx.doi.org/10.3390/microorganisms10020357.

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This study investigated nutrient removal from anaerobic digestion effluent by cultivating mixed-culture microalgae enriched from anaerobic sludge under different pH conditions: RUC (uncontrolled), R7–8 (maintained at 7–8), and R<8 (maintained below 8). Significant amounts of NH4+-N were lost by volatilization in RUC cultures due to increased pH values (≤8.6) during the early period of cultivation. The pH control strategies significantly affected the biological NH4+-N removal (highest in R7–8), microalgal growth (highest in R7–8), biomass settleability (highest in R<8), and microalgal gro

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4

Postaue, Najla, Leila Cristina Moraes, and Rosa Maria Farias Asmus. "CHORUME COMO FONTE DE NUTRIENTE NA PRODUÇÃO DA BIOMASSA MICROALGAL." e-xacta 12, no. 2 (2020): 11. http://dx.doi.org/10.18674/exacta.v12i2.2746.

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A biomassa de microalgas tem apresentado potencial para produção de biodiesel, contudo a viabilidade do cultivo de microalgas depende de fonte de nutrientes de baixo custo. O presente estudo objetivou utilizar o chorume como fonte de nutrientes para microalgas. Os experimentos foram conduzidos visando avaliar a obtenção da biomassa microalgal, conversão de lipídios e rendimento em ésteres metílicos de ácidos graxos, para os meios de cultivos utilizando 5%, 12% e 20% de chorume, com concentrações de 0,02, 0,05 e 0,08 g N. L-1 e para meio de controle contendo 1% de, Nitrogênio (N), Fósforo (P) e

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Van Anh, Le Thi, Tran Ngoc Anh Thu, and Nguyen Thi Dong Phuong. "Investigation of microalgae culture by autoflocculation methodologies." Vietnam Journal of Biotechnology 20, no. 3 (2022): 487–94. http://dx.doi.org/10.15625/1811-4989/17059.

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Harvesting of microalgae from their different cultivation media has pointed out challenges in resolving the problems of flocculation. These challenges must be faced with a suitable method for inducing flocculation that avoid or limit the microalgae’s contamination. This study developed the fundamental experiments with a support of chemicals and some bacteria strains inducing the flocculation of Chlorella vulgaris SAG 211-19. Particularly, the determination of minimum content of Mg2+, Ca2+, E. coli ATCC 85922 and Bacillus subtilis MT300405 was effectuated with co-cultivation of microalgae and s

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Sauvage, Justine, Gary H. Wikfors, Xiaoxu Li, et al. "Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species." Applied Microbiology and Biotechnology 105, no. 5 (2021): 2139–56. http://dx.doi.org/10.1007/s00253-021-11147-8.

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Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros cal

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7

Zhang, Xin Ru, Ze Yi Jiang, Hao Yuan, et al. "Influence of Surface Zeta Potential on Adhesion of Chlorella sp. to Substratum Surfaces." Advanced Materials Research 690-693 (May 2013): 1431–34. http://dx.doi.org/10.4028/www.scientific.net/amr.690-693.1431.

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Microalgae, in wet conditions, tend to grow on surfaces and form biofilms. The adhesion of microalgae to surfaces is very important for algal mass culture. The formation and development of microalgal biofims are in large denpend on the properties of cell surface, substratum surface and gowth medium. In this paper, the influence of substratum surface zeta potential on the microalgal biofilms was particularly investigated. We focused on a widely-used microalgal strain, the freshwater autotrophicChlorella sp..The adhesion phenomena ofChlorella sp.to surfaces with different zeta potential were obs

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8

Jeevanandam, Jaison, Mohd Razif Harun, Sie Yon Lau, Divine D. Sewu, and Michael K. Danquah. "Microalgal Biomass Generation via Electroflotation: A Cost-Effective Dewatering Technology." Applied Sciences 10, no. 24 (2020): 9053. http://dx.doi.org/10.3390/app10249053.

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Microalgae are an excellent source of bioactive compounds for the production of a wide range of vital consumer products in the biofuel, pharmaceutical, food, cosmetics, and agricultural industries, in addition to huge upstream benefits relating to carbon dioxide biosequestration and wastewater treatment. However, energy-efficient, cost-effective, and scalable microalgal technologies for commercial-scale applications are limited, and this has significantly impacted the full-scale implementation of microalgal biosystems for bioproduct development, phycoremediation, and biorefinery applications.

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9

Gonzalez-Bashan, Luz E., Vladimir K. Lebsky, Juan P. Hernandez, Jose J. Bustillos, and Yoav Bashan. "Changes in the metabolism of the microalga Chlorella vulgaris when coimmobilized in alginate with the nitrogen-fixing Phyllobacterium myrsinacearum." Canadian Journal of Microbiology 46, no. 7 (2000): 653–59. http://dx.doi.org/10.1139/w00-041.

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In an agroindustrial wastewater pond, a naturally occurring unicellular microalga, Chlorella vulgaris, was closely associated with the terrestrial plant-associative N2-fixing bacterium Phyllobacterium myrsinacearum. When the two microorganisms were artificially coimmobilized in alginate beads, they shared the same internal bead cavities, and the production of five microalgal pigments increased, but there were no effects on the number of the cells or the biomass of the microalga. The association, however, reduces the ability of C. vulgaris to remove ammonium ions and phosphorus from water. The

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10

Khaw, Yam Sim, Hui Teng Tan, Arissara Sopawong, Noor Azmi Shaharuddin, Abdul Rahman Omar, and Fatimah Md Yusoff. "A Recommendation for a Pre-Standardized Marine Microalgal Dry Weight Determination Protocol for Laboratory Scale Culture Using Ammonium Formate as a Washing Agent." Biology 10, no. 8 (2021): 799. http://dx.doi.org/10.3390/biology10080799.

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Microalgal biomass is one of the crucial criteria in microalgal studies. Many reported methods, even the well-established protocol on microalgal dry weight (DW) determination, vary greatly, and reliable comparative assessment amongst published results could be problematic. This study aimed to determine the best condition of critical parameters in marine microalgal DW determination for laboratory-scale culture using four different marine microalgal species. These parameters included the washing process, grades of glass microfiber filter (GMF), GMF pretreatment conditions, washing agent (ammoniu

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Kovalev, N. N., S. E. Leskova, E. V. Mikheev, Yu M. Pozdnyakova, and R. V. Esipenko. "CULTURAL AND BIOCHEMICAL PARAMETERS OF TWO MICROALGAE, PHAEODACTYLUM TRICORNUTUM AND TETRASELMIS SUECIACA, IN CUMULATIVE CULTURES." Bulletin оf Kamchatka State Technical University, no. 53 (2020): 54–65. http://dx.doi.org/10.17217/2079-0333-2020-53-54-65.

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A comparative study of cultural and biochemical parameters of two microalgal species, Tetraselmis sueciaca and Phaeodactylum tricornutum, was performed. Cell density in the storage culture was 2.88 million cells per one ml for T. sueciaca and 20.37 million cells per one ml for Ph. tricornutum. As shown, determination of op-tical density of the culture medium is a more objective characteristic of the cumulative culture growth. For T. sueciaca, cell growth rate during the course of experiment made 400% and for Ph. tricornutum – 700%. Dif-ference in the chemical composition of microalgal biomass

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12

Wang, Jing Han, Hai Zhen Yang, and Feng Wang. "Potential of Mixotrophic Cultivation of Chlorella sorokiniana for Biodiesel Production." Advanced Materials Research 779-780 (September 2013): 1509–13. http://dx.doi.org/10.4028/www.scientific.net/amr.779-780.1509.

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Biodiesel from microalgae provides a promising alternative for biofuel production. Microalgal biodiesel can be produced under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Studies of biodiesel production from microalgae have been reported mostly focusing on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity ofChlorella sorokinianaunder photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was adopted as organic

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13

Li, Shu Wen, Sheng Jun Luo, and Rong Bo Guo. "Influence of Carbon Dioxide Concentration on Microalgal Growth in a Bubble Column Photobioreactor." Advanced Materials Research 599 (November 2012): 137–40. http://dx.doi.org/10.4028/www.scientific.net/amr.599.137.

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The CO2 sequestration by microalgae is thought to be one of the most sustainable strategies to relieve global warming. To produce 1 ton of microalgal dry biomass, 2 ton of CO2 is required. However, insufficient supply of CO2 will limit microalgal growth, and excessive CO2 both means wasting and inhibits microalgal growth. In the present study, the dissolved CO2 concentration in culture limiting and inhibiting microalgal growth (Chlorella vulgaris) in a bubble column photobioreactor was studied. The experimental results showed that the dissolved CO2 concentration ranging from 107μmol/L to 1500

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14

Bertolini, Marta, and Fosca Conti. "Algae Culture Conditions and Process Parameters for Phycoremediation and Biomaterials Production." Environmental and Climate Technologies 26, no. 1 (2022): 1092–105. http://dx.doi.org/10.2478/rtuect-2022-0082.

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Abstract Climate change and increasing world population call for careful utilization of water and energy sources. Microalgae to treat wastewater in a coupled process to produce biofuels and other value-added products for human consumption are promising solutions. An analysis of culture parameters and cultivation processes is presented as essential to achieve economical sustainability from the algae. Results of the activity of microalgal strains in detoxification of wastewater are compared and discussed, particularly in remediation of nitrogen and phosphorous compounds, heavy metal, pharmaceuti

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15

Fitriyah, F., Y. Faramitha, D. A. Sari, I. Kresnawaty, T. Panji, and D. Santoso. "Improved direct lysis PCR amplification method of microalgal culture for sequencing and species identification." IOP Conference Series: Earth and Environmental Science 948, no. 1 (2021): 012013. http://dx.doi.org/10.1088/1755-1315/948/1/012013.

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Abstract Molecular approach plays important role in species identification for microalgae which involves sequencing of specific DNA barcode present in the genome. This approach involved preparation of template DNA for polymerase chain reaction (PCR) which is time consuming and requires large amounts of algal cells. Microalgal direct PCR have been used frequently for species identification, which simplified the DNA isolation procedure. However, the recent attempts to amplify the rbcL gene of microalga using the previously reported protocol led to poor repeatability. In this study, Nannochlorops

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Wang, Jing Han, Hai Zhen Yang, and Feng Wang. "Mixotrophic Cultivation of Scenedesmus sp. as Biodiesel Feedstock." Advanced Materials Research 777 (September 2013): 268–73. http://dx.doi.org/10.4028/www.scientific.net/amr.777.268.

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Microalgae are a promising feedstock for biodiesel production. Microalgal biodiesel can be obtained under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Reported studies of microalgal biodiesel production are mainly based on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity of Scenedesmus sp. under photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was added as organic carbon source at five concentrations (0

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17

Branyikova, Irena, Gita Prochazkova, Tomas Potocar, Zuzana Jezkova, and Tomas Branyik. "Harvesting of Microalgae by Flocculation." Fermentation 4, no. 4 (2018): 93. http://dx.doi.org/10.3390/fermentation4040093.

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Due to increasing demands for microalgal biomass and products originating from microalgae, large-scale production systems are necessary. However, current microalgal production technologies are not cost-effective and are hindered by various bottlenecks, one of which is the harvesting of microalgal biomass. Cell separation is difficult because of the low sedimentation velocity of microalgae, their colloidal character with repelling negative surface charges, and low biomass concentrations in culture broths; therefore, large volumes need to be processed in order to concentrate the cells. Flocculat

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18

Ermis, Hande, Ünzile Güven-Gülhan, Tunahan Çakır, and Mahmut Altınbaş. "Microalgae growth and diversity in anaerobic digestate compared to synthetic media." Biofuel Research Journal 9, no. 1 (2022): 1551–61. http://dx.doi.org/10.18331/brj2022.9.1.2.

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Economizing microalgal cultivation is a considerable milestone targeted by efforts put into microalgal biorefineries. In light of that, the present study was aimed to explore the potential of using anaerobic liquid digestate (ALD) as culture media to grow microalgae and compared it with three different synthetic media (i.e., N8, BBM, and M8) in terms of biomass yield, fatty acid composition, and nutrient utilization/recovery. Moreover, a mixed culture of wild-type microalgae was employed in this study owing to the ability of mixed cultures to survive extreme conditions, eliminating the risk of

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19

Hao, Zong Di, Ping Huai Liu, Xun Yang, Jie Shi, and Sen Zhang. "Screening Method for Lipid-Content Microalgae Based on Sulfo-Phospho-Vanillin Reaction." Advanced Materials Research 610-613 (December 2012): 3532–35. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.3532.

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Studies that address the use of microalgae as biofuels often require the frequent measurement of total lipid content. Traditional methods for the quantiﬁcation of lipid are time-consuming or involve the use of expensive analytical equipment that is not available in many labs. Here we investigated microalgal culture as the starting material and simple, colorimetric method for quantitative measurement of neutral lipids in microalgae with a relatively high correlation coefﬁcient (R2=0.9038) between gravimetric and spectrophotometric quantiﬁcation. Linear responses for triolein, vegetable oil and

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20

Tang, Zhixuan, Jiangbing Qiu, Guixiang Wang, Ying Ji, Philipp Hess, and Aifeng Li. "Development of an Efficient Extraction Method for Harvesting Gymnodimine-A from Large-Scale Cultures of Karenia selliformis." Toxins 13, no. 11 (2021): 793. http://dx.doi.org/10.3390/toxins13110793.

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Gymnodimine-A (GYM-A) is a fast-acting microalgal toxin and its production of certified materials requires an efficient harvesting technology from the large-scale cultures of toxigenic microalgae. In this study the recoveries of GYM-A were compared between several liquid-liquid extraction (LLE) treatments including solvents, ratios and stirring times to optimize the LLE technique for harvesting GYM-A from Karenia selliformis cultures, of which the dichloromethane was selected as the extractant and added to microalgal cultures at the ratio 55 mL L−1 (5.5%, v/v). The recovery of GYM-A obtained b

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Ma, Wei, Chenchen Feng, Fachun Guan, Dianrong Ma, and Jinling Cai. "Effective Chlorella vulgaris Biomass Harvesting through Sulfate and Chloride Flocculants." Journal of Marine Science and Engineering 11, no. 1 (2022): 47. http://dx.doi.org/10.3390/jmse11010047.

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Efficient microalgae harvesting is a great challenge hindering diverse industrial applications of microalgae. Flocculation is regarded as an effective and promising technology for microalgae harvesting. In this study, sulfate (Al2(SO4)3 and Fe2(SO4)3) and chloride flocculants (AlCl3 and FeCl3) were used to harvest Chlorella vulgaris. Flocculation conditions, including flocculant dose, flocculation time, stirring speed, stirring time, and flocculation pH, were optimized, and flocculant effects on microalgal cell status, floc characteristics, biomass composition, algal cell re-culture, and media

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Araújo, Fabíola Ornellas de, Reinaldo Giudici, and João José Martins Simões de Sousa. "CULTIVATION OF THE MICROALGAE CHLORELLA PYRENOIDOSA USING THE PROCESSES OF BIOTECHNOLOGY." Revista Eletrônica Acervo Científico 2 (March 26, 2019): 121. http://dx.doi.org/10.25248/reac.e121.2019.

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The results obtained here, show that the use of Chlorella pyrenoidosa microalgae and biotechnology, using the discontinuous process, presented satisfactory results. With this, the study of the microalga Chlorella sp. has proved to be important because it has a wealth of proteins, carbohydrates, amino acids, fatty acids, carotenoids, vitamins and minerals in its constitution, which may represent commercial importance. This research revealed the best results for obtaining a lipoprotein-rich biomass, taking into account three different culture media, calculations of cell concentration, cell produ

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Journal, Baghdad Science. "Microalgae Chlorella Vulgaris Harvesting Via Co-Pelletization with Filamentous Fungus." Baghdad Science Journal 15, no. 1 (2018): 31–36. http://dx.doi.org/10.21123/bsj.15.1.31-36.

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The objective of this study was to progress another method for coagulation/flocculation of the microalga Chlorella vulgaris via pellet-forming of the fungal species Aspergillus niger which was isolated from municipal wastewater mud and the facultative heterotrophic microalga "C.vulgaris was used. The main factors studies were spore inoculums, organic carbon concentration in medium as well as pH variation which had considerably positive effects on microalgae/fungi co-pelletization formation. The process parameters are an inoculum1×104 spores/ML, 15 g/l sucrose as carbon source and pH ranged fro

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Sirohi, Priyanka, Hariom Verma, Sandeep Kumar Singh, et al. "Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production." Current Issues in Molecular Biology 44, no. 12 (2022): 6257–79. http://dx.doi.org/10.3390/cimb44120427.

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Microalgae are microscopic photosynthetic organisms frequently found in fresh and marine water ecosystems. Various microalgal species have been considered a reservoir of diverse health-value products, including vitamins, proteins, lipids, and polysaccharides, and are broadly utilized as food and for the treatment of human ailments such as cancer, cardiovascular diseases, allergies, and immunodeficiency. Microalgae-derived carotenoids are the type of accessory pigment that possess light-absorbing potential and play a significant role in metabolic functions. To date, nearly a thousand carotenoid

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Plyusnina, Tatiana Yu, Sergei S. Khruschev, Polina V. Fursova, et al. "Simulating the Interplay between the Uptake of Inorganic Phosphate and the Cell Phosphate Metabolism under Phosphorus Feast and Famine Conditions in Chlorella vulgaris." Cells 10, no. 12 (2021): 3571. http://dx.doi.org/10.3390/cells10123571.

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Using a mathematical simulation approach, we studied the dynamics of the green microalga Chlorella vulgaris phosphate metabolism response to shortage and subsequent replenishing of inorganic phosphate in the medium. A three-pool interaction model was used to describe the phosphate uptake from the medium, its incorporation into the cell organic compounds, its storage in the form of polyphosphates, and culture growth. The model comprises a system of ordinary differential equations. The distribution of phosphorous between cell pools was examined for three different stages of the experiment: growt

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Fuentes, Juan-Luis, Zaida Montero, María Cuaresma, et al. "Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production." Processes 8, no. 3 (2020): 324. http://dx.doi.org/10.3390/pr8030324.

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The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g−1dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L−1 d−1 was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content o

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Moreira, Juliana Botelho, Suelen Goettems Kuntzler, Priscilla Quenia Muniz Bezerra, et al. "Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants." Polysaccharides 3, no. 1 (2022): 264–76. http://dx.doi.org/10.3390/polysaccharides3010015.

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Microalgae are used in flocculation processes because biopolymers are released into the culture medium. Microalgal cell growth under specific conditions (temperature, pH, luminosity, nutrients, and salinity) provides the production and release of exopolysaccharides (EPS). These biopolymers can be recovered from the medium for application as bioflocculants or used directly in cultivation as microalgae autoflocculants. The optimization of nutritional parameters, the control of process conditions, and the possibility of scaling up allow the production and industrial application of microalgal EPS.

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de Morais, Michele Greque, Bruna da Silva Vaz, Etiele Greque de Morais, and Jorge Alberto Vieira Costa. "Biologically Active Metabolites Synthesized by Microalgae." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/835761.

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Microalgae are microorganisms that have different morphological, physiological, and genetic traits that confer the ability to produce different biologically active metabolites. Microalgal biotechnology has become a subject of study for various fields, due to the varied bioproducts that can be obtained from these microorganisms. When microalgal cultivation processes are better understood, microalgae can become an environmentally friendly and economically viable source of compounds of interest, because production can be optimized in a controlled culture. The bioactive compounds derived from micr

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Li, Jiawei, Xinqing Zhao, Jo-Shu Chang, and Xiaoling Miao. "A Two-Stage Culture Strategy for Scenedesmus sp. FSP3 for CO2 Fixation and the Simultaneous Production of Lutein under Light and Salt Stress." Molecules 27, no. 21 (2022): 7497. http://dx.doi.org/10.3390/molecules27217497.

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In this study, Scenedesmus sp. FSP3 was cultured using a two-stage culture strategy for CO2 fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO2 introduced to promote the rapid growth and CO2 fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L−1 and a light intensity of 160 μmol m−2 s−1 were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO2 fixation were simultaneously achieved. The biomass productivity a

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Babiak, Wioleta, and Izabela Krzemińska. "Extracellular Polymeric Substances (EPS) as Microalgal Bioproducts: A Review of Factors Affecting EPS Synthesis and Application in Flocculation Processes." Energies 14, no. 13 (2021): 4007. http://dx.doi.org/10.3390/en14134007.

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Microalgae are natural resources of intracellular compounds with a wide spectrum of applications in, e.g., the food industry, pharmacy, and biofuel production. The extracellular polymeric substances (EPS) released by microalgal cells are a valuable bioproduct. Polysaccharides, protein, lipids, and DNA are the main constituents of EPS. This review presents the recent advances in the field of the determinants of the synthesis of extracellular polymeric substances by microalgal cells and the EPS structure. Physical and chemical culture conditions have been analyzed to achieve useful insights into

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Iasimone, F., G. Zuccaro, V. D'Oriano, et al. "Combined yeast and microalgal cultivation in a pilot-scale raceway pond for urban wastewater treatment and potential biodiesel production." Water Science and Technology 77, no. 4 (2017): 1062–71. http://dx.doi.org/10.2166/wst.2017.620.

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Abstract A mixed culture of oleaginous yeast Lipomyces starkeyi and wastewater native microalgae (mostly Scenedesmus sp. and Chlorella sp.) was performed to enhance lipid and biomass production from urban wastewaters. A 400 L raceway pond, operating outdoors, was designed and used for biomass cultivation. Microalgae and yeast were inoculated into the cultivation pond with a 2:1 inoculum ratio. Their concentrations were monitored for 14 continuous days of batch cultivation. Microalgal growth presented a 3-day initial lag-phase, while yeast growth occurred in the first few days. Yeast activity d

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Ortiz-Marquez, Juan Cesar Federico, Mauro Do Nascimento, Maria de los Angeles Dublan, and Leonardo Curatti. "Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae." Applied and Environmental Microbiology 78, no. 7 (2012): 2345–52. http://dx.doi.org/10.1128/aem.06260-11.

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ABSTRACTConcerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third-generation biofuels from microalgal biomass mainly because of its potential for high yields and reduced land use changes in comparison with biofuels derived from plant feedstocks. Regardless of the nature of the feedstock, the use of fertilizers, especially nitrogen, enta

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Barua, Visva Bharati, and Mariya Munir. "A Review on Synchronous Microalgal Lipid Enhancement and Wastewater Treatment." Energies 14, no. 22 (2021): 7687. http://dx.doi.org/10.3390/en14227687.

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Microalgae are unicellular photosynthetic eukaryotes that can treat wastewater and provide us with biofuel. Microalgae cultivation utilizing wastewater is a promising approach for synchronous wastewater treatment and biofuel production. However, previous studies suggest that high microalgae biomass production reduces lipid production and vice versa. For cost-effective biofuel production from microalgae, synchronous lipid and biomass enhancement utilizing wastewater is necessary. Therefore, this study brings forth a comprehensive review of synchronous microalgal lipid and biomass enhancement st

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Savio, Saverio, Corrado di Natale, Roberto Paolesse, Larisa Lvova, and Roberta Congestri. "Keeping Track of Phaeodactylum tricornutum (Bacillariophyta) Culture Contamination by Potentiometric E-Tongue." Sensors 21, no. 12 (2021): 4052. http://dx.doi.org/10.3390/s21124052.

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The large-scale cultivation of microalgae provides a wide spectrum of marketable bioproducts, profitably used in many fields, from the preparation of functional health products and feed supplement in aquaculture and animal husbandry to biofuels and green chemistry agents. The commercially successful algal biomass production requires effective strategies to maintain the process at desired productivity and stability levels. Hence, the development of effective early warning methods to timely indicate remedial actions and to undertake countermeasures is extremely important to avoid culture collaps

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Marazzi, Francesca, Micol Bellucci, Tania Fantasia, Elena Ficara, and Valeria Mezzanotte. "Interactions between Microalgae and Bacteria in the Treatment of Wastewater from Milk Whey Processing." Water 12, no. 1 (2020): 297. http://dx.doi.org/10.3390/w12010297.

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Milk whey processing wastewaters (MWPWs) are characterized by high COD and organic nitrogen content; the concentrations of phosphorus are also relevant. A microalgal-based process was tested at lab scale in order to assess the feasibility of treating MWPW without any dilution or pre-treatment. Different microalgal strains and populations were tested. Based on the obtained results, Scenedesmus acuminatus (SA) and a mixed population (PM) chiefly made of Chlorella, Scenedesmus, and Chlamydomonas spp. were grown in duplicate for 70 days in Plexiglas column photobioreactors (PBRs), fed continuously

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Stemmler, Kevin, Rebecca Massimi, and Andrea E. Kirkwood. "Growth and fatty acid characterization of microalgae isolated from municipal waste-treatment systems and the potential role of algal-associated bacteria in feedstock production." PeerJ 4 (March 7, 2016): e1780. http://dx.doi.org/10.7717/peerj.1780.

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Much research has focused on growing microalgae for biofuel feedstock, yet there remain concerns about the feasibility of freshwater feedstock systems. To reduce cost and improve environmental sustainability, an ideal microalgal feedstock system would be fed by municipal, agricultural or industrial wastewater as a main source of water and nutrients. Nonetheless, the microalgae must also be tolerant of fluctuating wastewater quality, while still producing adequate biomass and lipid yields. To address this problem, our study focused on isolating and characterizing microalgal strains from three m

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Supono, Supono, Maria Mugica, Stefan Spreitzenbarth, and Andrew Jeffs. "Potential for Concentrated Microalgae as Replacement Diets for Juvenile Green-Lipped Mussels, Perna canaliculus." Aquaculture Research 2023 (February 21, 2023): 1–9. http://dx.doi.org/10.1155/2023/9841172.

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The nursery culture of bivalves typically relies on the feeding of costly live microalgae, while the use of natural sources of phytoplankton for feed is uncertain due to their variable quality and abundance. Replacement diets have been applied in bivalve nursery culture to replace live microalgae with varying success. This study investigated the potential use of two concentrated microalgal diets at a range of levels of substitution with live microalgae. Shellfish Diet 1800® (called SD) and LPB™ Frozen Shellfish Diet® (called LPB) were fed to juvenile green-lipped mussels (Perna canaliculus) at

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Zieliński, Marcin, Marcin Dębowski, and Joanna Kazimierowicz. "Outflow from a Biogas Plant as a Medium for Microalgae Biomass Cultivation—Pilot Scale Study and Technical Concept of a Large-Scale Installation." Energies 15, no. 8 (2022): 2912. http://dx.doi.org/10.3390/en15082912.

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Microalgae-based technologies have huge potential for application in the environment sector and the bio-energy industry. However, their cost-efficiency has to be improved by drawing on design and operation data for large-scale installations. This paper presents a technical concept of an installation for large-scale microalgae culture on digestate liquor, and the results of a pilot-scale study to test its performance. The quality of non-treated digestate has been shown to be insufficient for direct use as a growth medium due to excess suspended solids, turbidity, and organic matter content, whi

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DeWreede, Robert E. "SEAWEEDS, MARINE AQUACULTURE, AND MICROALGAL CULTURE." Journal of Phycology 40, no. 5 (2004): 998. http://dx.doi.org/10.1111/j.1529-8817.2004.40503.x.

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Avsiyan, Anna L., and Alexander S. Lelekov. "Dependence of microalgae culture specific endogenous loss rate and gross productivity on irradiance." Issues of modern algology (Вопросы современной альгологии), no. 1(22) (2020): 8–16. http://dx.doi.org/10.33624/2311-0147-2020-1(22)-8-16.

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Microalgal growth rate is determined by the difference between gross productivity and endogenous biomass loss rate. Gross productivity is a function of surface irradiance and reaches the maximal value under saturating light intensity. Endogenous biomass consumption of microalgal cells is mainly due to the respiration which can be sub-divided into growth and maintenance respiration. The paper proposes equations for calculating microalgae culture gross productivity and specific loss rate based on production characteristics. Verification of the obtained equations was performed for Arthrospira pla

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Hung, Ha Vinh. "RESEARCH ON HARVESTING OF MICROALGAE CHLORELLA SP. BY ELECTROCHEMICAL FLOTATION METHOD USING CORROSIVE ELECTRODES." Vietnam Journal of Science and Technology 55, no. 4C (2018): 14. http://dx.doi.org/10.15625/2525-2518/55/4c/12123.

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Microalgae are a promising feedstock for biodiesel production. Harvesting of microalgal biomass is still a bottleneck to its commercial scale application, due to small cell size, low culture densities, colloidal stability and thus economic disadvantage. The aim of this study was to evaluate the biomass separation of the small size microalgae Chlorella sp. by electrochemical flotation process with rectangle electrodes using aluminum or iron plates. The most effective conditions for this experiment involved the use of an aluminum electrode for 30 min with a current density of 1.5 mA/cm2, whereas

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Jo, Seung-Woo, Jeong-Mi Do, Ho Na, Ji Won Hong, Il-Sup Kim, and Ho-Sung Yoon. "Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation." PeerJ 8 (July 16, 2020): e9418. http://dx.doi.org/10.7717/peerj.9418.

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Metagenome studies have provided us with insights into the complex interactions of microorganisms with their environments and hosts. Few studies have focused on microalgae-associated metagenomes, and no study has addressed aquatic microalgae and their bacterial communities in open pond raceways (OPRs). This study explored the possibility of using microalgal biomasses from OPRs for biodiesel and biofertilizer production. The fatty acid profiles of the biomasses and the physical and chemical properties of derived fuels were evaluated. In addition, the phenotype-based environmental adaptation abi

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Macdonald Miller, Sean, Raffaela M. Abbriano, Anna Segecova, Andrei Herdean, Peter J. Ralph, and Mathieu Pernice. "Comparative Study Highlights the Potential of Spectral Deconvolution for Fucoxanthin Screening in Live Phaeodactylum tricornutum Cultures." Marine Drugs 20, no. 1 (2021): 19. http://dx.doi.org/10.3390/md20010019.

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Microalgal biotechnology shows considerable promise as a sustainable contributor to a broad range of industrial avenues. The field is however limited by processing methods that have commonly hindered the progress of high throughput screening, and consequently development of improved microalgal strains. We tested various microplate reader and flow cytometer methods for monitoring the commercially relevant pigment fucoxanthin in the marine diatom Phaeodactylum tricornutum. Based on accuracy and flexibility, we chose one described previously to adapt to live culture samples using a microplate rea

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Policastro, Grazia, Alessandra Cesaro, Massimiliano Fabbricino, and Francesco Pirozzi. "Opportunities and Challenges from Symbiosis of Agro-Industrial Residue Anaerobic Digestion with Microalgae Cultivation." Sustainability 14, no. 23 (2022): 15607. http://dx.doi.org/10.3390/su142315607.

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During the last few years, many studies have tested microalgal systems for nitrogen removal from the digestate. However, most of these studies were carried out using pure culture microalgal strains, which require aseptic conditions and thus cannot be used in full-scale applications. The aim of the present study was to explore opportunities in and challenges of the industrial symbiosis of anaerobic digestion and microalgae cultivation to enhance agro-industrial residue management. Batch tests were carried out to investigate the use of a mixed (open) microalgal consortium to treat the liquid fra

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Khaw, Yam Sim, Fatimah Md Yusoff, Hui Teng Tan, et al. "Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review." Marine Drugs 20, no. 10 (2022): 592. http://dx.doi.org/10.3390/md20100592.

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Fucoxanthin is one of the light-harvesting pigments in brown microalgae, which is increasingly gaining attention due to its numerous health-promoting properties. Currently, the production of microalgal fucoxanthin is not yet feasible from an economic perspective. However, the cultivation of microalgae at favourable conditions holds great potential to increase the viability of this fucoxanthin source. Hence, this study aimed to review the fucoxanthin production of microalgae under different conditions systematically. A literature search was performed using the Web of Science, Scopus and PubMed

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Greenwell, H. C., L. M. L. Laurens, R. J. Shields, R. W. Lovitt, and K. J. Flynn. "Placing microalgae on the biofuels priority list: a review of the technological challenges." Journal of The Royal Society Interface 7, no. 46 (2009): 703–26. http://dx.doi.org/10.1098/rsif.2009.0322.

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Microalgae provide various potential advantages for biofuel production when compared with ‘traditional’ crops. Specifically, large-scale microalgal culture need not compete for arable land, while in theory their productivity is greater. In consequence, there has been resurgence in interest and a proliferation of algae fuel projects. However, while on a theoretical basis, microalgae may produce between 10- and 100-fold more oil per acre, such capacities have not been validated on a commercial scale. We critically review current designs of algal culture facilities, including photobioreactors and

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Nguyen, Hong Hai, Hong Ngoc Luong, Ngoc Kim Qui Nguyen, et al. "Effects of settling time on the flocculation progress and treatment performance in the co-culture of microalgae-activated sludge photobioreactor." Ministry of Science and Technology, Vietnam 64, no. 4 (2022): 91–95. http://dx.doi.org/10.31276/vjste.64(4).91-95.

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The application of microalgae-based wastewater treatment systems using wastewater as a source of nutrients has been success-fully developed in recent years and has brought about positive results in wastewater treatment and microalgal biomass recovery while producing valuable products. This study presents the application of a microalgae and activated sludge (AS) co-culture in a low agitation photobioreactor (aPBR), which could reduce energy usage. In addition, the results demonstrate the role of settling time on co-culture flocculation progress and wastewater treatment performance. The average

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Yu, Byung Sun, Young Joon Sung, Min Eui Hong, and Sang Jun Sim. "Improvement of Photoautotrophic Algal Biomass Production after Interrupted CO2 Supply by Urea and KH2PO4 Injection." Energies 14, no. 3 (2021): 778. http://dx.doi.org/10.3390/en14030778.

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Microalgae-derived biomass is currently considered a sustainable feedstock for making biofuels, including biodiesel and direct combustion fuel. The photoautotrophic cultivation of microalgae using flue gas from power plants has been continuously investigated to improve the economic feasibility of microalgae processes. The utilization of waste CO2 from power plants is advantageous in reducing carbon footprints and the cost of carbon sources. Nonetheless, the sudden interruption of CO2 supply during microalgal cultivation leads to a severe reduction in biomass productivity. Herein, chemical fert

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Sarwa, Prakash, and Sanjay Kumar Verma. "Identification and Characterization of Green Microalgae, Scenedesmus sp. MCC26 and Acutodesmus obliquus MCC33 Isolated From Industrial Polluted Site Using Morphological and Molecular Markers." International Journal of Applied Sciences and Biotechnology 5, no. 4 (2017): 415–22. http://dx.doi.org/10.3126/ijasbt.v5i4.18083.

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Two unicellular green microalgae, were isolated from Amani Shah Nallah (Jaipur, India) that receives discharge of dyeing industries. Preliminary morphological characterization using light microscopy showed ovoid, fusiform shape of cells with single to two celled coenobia in a culture suspension. Transmission electron microscopy (TEM) analyses depict abundant chloroplast located at the periphery of the cell, a pyrenoid as well as nucleus is visible in the centre. A well developed cell wall with many layers is also evident in TEM. Phylogenetic position and genetic variabilty among the isolated m

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Li, Gang, Yuhang Hao, Tenglun Yang, et al. "Enhancing Bioenergy Production from the Raw and Defatted Microalgal Biomass Using Wastewater as the Cultivation Medium." Bioengineering 9, no. 11 (2022): 637. http://dx.doi.org/10.3390/bioengineering9110637.

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Improving the efficiency of using energy and decreasing impacts on the environment will be an inevitable choice for future development. Based on this direction, three kinds of medium (modified anaerobic digestion wastewater, anaerobic digestion wastewater and a standard growth medium BG11) were used to culture microalgae towards achieving high-quality biodiesel products. The results showed that microalgae culturing with anaerobic digestate wastewater could increase lipid content (21.8%); however, the modified anaerobic digestion wastewater can boost the microalgal biomass production to 0.78 ±

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