Journal articles: 'Microalgues – Biotechnologie'

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Ramírez, M. E., Y. H. Vélez, L. Rendón, and E. Alzate. "Potential of microalgae in the bioremediation of water with chloride content." Brazilian Journal of Biology 78, no. 3 (October 23, 2017): 472–76. http://dx.doi.org/10.1590/1519-6984.169372.

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Abstract In this work it was carried out the bioremediation of water containing chlorides with native microalgae (MCA) provided by the Centre for study and research in biotechnology (CIBIOT) at Universidad Pontificia Bolivariana. Microalgae presented an adaptation to the water and so the conditions evaluated reaching a production of CO2 in mg L-1 of 53.0, 26.6, 56.0, 16.0 and 30.0 and chloride removal efficiencies of 16.37, 26.03, 40.04, 25.96 and 20.25% for microalgae1, microalgae2, microalgae3, microalgae4 and microalgae5 respectively. Water bioremediation process was carried out with content of chlorides in fed batch system with an initial concentration of chlorides of 20585 mg L-1 every 2 days. The Manipulated variables were: the flow of MCA3 (10% inoculum) for test one; NPK flow for test two, and flow of flow of MCA3+0.5 g L-1 NPK. Chloride removal efficiencies were 66.88%, 63.41% and 66.98% for test one, two and three respectively, for a total bioprocess time of 55 days.

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Bosso, Alessandra, Naiale Fernanda Da Silva Veloso, Camila Fernanda Alba, Josemeyre Bonifácio Da Silva, Luiz Rodrigo Ito Morioka, and Helio Hiroshi Suguimoto. "Microalgae Grown in Cheese Whey and β-galactosidase Production." Ensaios e Ciência C Biológicas Agrárias e da Saúde 24, no. 3 (October 26, 2020): 268–72. http://dx.doi.org/10.17921/1415-6938.2020v24n3p268-272.

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O soro de queijo é o principal subproduto da indústria de laticínios e a alta demanda biológica e química de oxigênio (DBO e DQO) pode causar vários problemas ambientais. Estudos recentes apontam os potenciais usos biotecnológicos do soro de queijo, como o meio de fermentação, para a produção de β-galactosidase. A enzima é muito importante para hidrólise da lactose em galactose e glicose, monossacarídeos mais digeríveis pelo organismo humano. As microalgas podem produzir a β-galactosidase através de processos fermentativos. O objetivo da presente revisão é descrever sucintamente o progresso recente sobre o uso de microalgas na produção de β-galactosidase. No geral, o artigo resume o estado atual do conhecimento sobre microalgas, beta-galactosidase e soro de queijo como fonte de carbono para o crescimento de microalgas e dentro do conceito de economia circular. No entanto, ainda são necessários estudos adicionais sobre as melhores condições de cultivo de microalgas com o objetivo de produzir a enzima em questão. Palavras-chave: Indústria de lacticínio. Biotecnologia. Economia Circular. Valor Agregado. Abstract Cheese whey is the main by-product of dairy industry and due to high biological and chemical oxygen demands (BOD and COD) can cause several environmental problems. Recent studies have pointed the biotechnological potential uses of cheese whey such as fermentation medium to the β-galactosidase production. The enzyme is very important to breakdown the lactose into galactose and glucose, monosaccharide sugars more digestible than lactose. Microalgae can produce β-galactosidase through fermentative processes. The purpose of the current mini-review is to succinctly describe recent progress about the use of microalgae to β-galactosidase production. Overall, the paper summarizes the current state of knowledge about microalgae, beta-galactosidase and cheese whey as carbon source to growing of microalgae and within circular economy concept. However, there is still a need for further studies regarding the best microalgae cultivation conditions with the objective of producing the enzyme in question. Keywords: Dairy industry. Biotechnology. Circular Economy. Added Value.

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Benemann, John R., David M. Tillett, and Joseph C. Weissman. "Microalgae biotechnology." Trends in Biotechnology 5, no. 2 (February 1987): 47–53. http://dx.doi.org/10.1016/0167-7799(87)90037-0.

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Ching, Wong Y., and Nur A. Shukri. "Investigations of Light Intensities, Nutrient, and Carbon Sources Towards Microalgae Oil Production via Soxhlet Extraction Techniques." Current Biotechnology 10, no. 1 (May 20, 2021): 46–54. http://dx.doi.org/10.2174/2211550110666210204151145.

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Aims: This study was carried out to study the optimized condition for microalgae cultivation in terms of light intensity, and nutrient supply. Also, use of a carbon source was studied to optimize the microalgae growth to produce microalgae with a high biomass productivity and a high lipid content. Background: Algae can be categorized into macroalgae and microalgae. Commonly, microalgae are used to produce biodiesel since microalgae can yield 5000-15000 of oil gallons compared to plant-based biomass as feedstock produced 50-500 oil gallon. Furthermore, microalgae do not face any food crisis and can be cultivated in any wasteland that is not suitable for agriculture throughout the year, compared to crops. Microalgae can also be cultivated in freshwater, saline water and wastewater. Methods: Microalgae cultivation was carried out with microalgae culture labelled as MX1, MX2, MX3, MX4 and were cultivated under high light intensities, whereas MY1, MY2, MY3, MY4 were cultivated under medium light intensity and MZ1, MZ2, MZ3 MZ4 became control culture that was cultivated under high light intensities and no light condition. Results: The effect of light intensity, NPK fertilizer, and glucose on microalgae’s biomass production will be observed simultaneously. At the end of cultivation, MX2 obtained the highest biomass of 97.186 g. The oil extraction yield is 9.66%. GC-MS analysis showed the presence of UFA and PUFA in the oil. Conclusion: Thus, future research is needed to improve the technique to increase the microalgae biomass and lipid to become the potential feedstock for the production of biodiesel.

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Vasilieva, S. G., E. S. Lobakova, A. A. Lukyanov, and A. E. Solovchenko. "Immobilized microalgae in biotechnology." Moscow University Biological Sciences Bulletin 71, no. 3 (July 2016): 170–76. http://dx.doi.org/10.3103/s0096392516030135.

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Turner, Michael. "Microalgae — biotechnology and microbiology." Journal of Experimental Marine Biology and Ecology 183, no. 2 (November 1994): 300–301. http://dx.doi.org/10.1016/0022-0981(94)90095-7.

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Valverde, Federico, Francisco J. Romero-Campero, Rosa León, Miguel G. Guerrero, and Aurelio Serrano. "New challenges in microalgae biotechnology." European Journal of Protistology 55 (August 2016): 95–101. http://dx.doi.org/10.1016/j.ejop.2016.03.002.

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Abu-Ghosh, Said, Dror Fixler, Zvy Dubinsky, and David Iluz. "Flashing light in microalgae biotechnology." Bioresource Technology 203 (March 2016): 357–63. http://dx.doi.org/10.1016/j.biortech.2015.12.057.

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Pritchard, Hayden N. "Microalgae: Biotechnology and Microbiology.E. W. Becker." Quarterly Review of Biology 70, no. 3 (September 1995): 350–51. http://dx.doi.org/10.1086/419123.

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Pulz, Otto, and Wolfgang Gross. "Valuable products from biotechnology of microalgae." Applied Microbiology and Biotechnology 65, no. 6 (August 6, 2004): 635–48. http://dx.doi.org/10.1007/s00253-004-1647-x.

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Wang, Hui, Haywood D. Laughinghouse, Matthew A. Anderson, Feng Chen, Ernest Willliams, Allen R. Place, Odi Zmora, Yonathan Zohar, Tianling Zheng, and Russell T. Hill. "Novel Bacterial Isolate from Permian Groundwater, Capable of Aggregating Potential Biofuel-Producing Microalga Nannochloropsis oceanica IMET1." Applied and Environmental Microbiology 78, no. 5 (December 22, 2011): 1445–53. http://dx.doi.org/10.1128/aem.06474-11.

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ABSTRACTIncreasing petroleum costs and climate change have resulted in microalgae receiving attention as potential biofuel producers. Little information is available on the diversity and functions of bacterial communities associated with biofuel-producing algae. A potential biofuel-producing microalgal strain,Nannochloropsis oceanicaIMET1, was grown in Permian groundwater. Changes in the bacterial community structure at three temperatures were monitored by two culture-independent methods, and culturable bacteria were characterized. After 9 days of incubation,N. oceanicaIMET1 began to aggregate and precipitate in cultures grown at 30°C, whereas cells remained uniformly distributed at 15°C and 25°C. The bacterial communities in cultures at 30°C changed markedly. Some bacteria isolated only at 30°C were tested for their potential for aggregating microalgae. A novel bacterium designated HW001 showed a remarkable ability to aggregateN. oceanicaIMET1, causing microalgal cells to aggregate after 3 days of incubation, while the total lipid content of the microalgal cells was not affected. Direct interaction of HW001 andN. oceanicais necessary for aggregation. HW001 can also aggregate the microalgaeN. oceanicaCT-1,Tetraselmis suecica, andT. chuiias well as the cyanobacteriumSynechococcusWH8007. 16S rRNA gene sequence comparisons indicated the great novelty of this strain, which exhibited only 89% sequence similarity with any previously cultured bacteria. Specific primers targeted to HW001 revealed that the strain originated from the Permian groundwater. This study of the bacterial communities associated with potential biofuel-producing microalgae addresses a little-investigated area of microalgal biofuel research and provides a novel approach to harvest biofuel-producing microalgae by using the novel bacterium strain HW001.

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Kulachinskaya, A. Yu, N. A. Politaeva, Yu A. Smyatskaya, K. R. Tarantseva, and T. Yu Kudryavtseva. "Development of Cost-Effective Biotechnology for Extracting the Total Lipids from the Biomass of the Microalgae Chlorella Sorokiniana." Ecology and Industry of Russia 24, no. 10 (October 14, 2020): 38–42. http://dx.doi.org/10.18412/1816-0395-2020-10-38-42.

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The flow chart of the processes of lipid extraction from the biomass of the microalgae Chlorella sorokiniana is presented. The main equipment for the production of products from the biomass of the microalgae Chlorella sorokiniana was selected, and economic calculations were carried out, which made it possible to draw a conclusion about the amount of costs for the production of lipids from the dry biomass of the microalgae Chlorella sorokiniana. The obtained indicators allow us to consider the proposed biotechnology as cost effective.

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Melikhov, V. V., L. N. Medvedeva, M. V. Frolova, and S. S. Shalaeva. "Entrepreneurial platform for the development of microalgae biotechnologies." IOP Conference Series: Earth and Environmental Science 577 (October 15, 2020): 012002. http://dx.doi.org/10.1088/1755-1315/577/1/012002.

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Echeverri, Danilo, Juliana Romo, Néstor Giraldo, and Lucía Atehortúa. "Microalgae protoplasts isolation and fusion for biotechnology research." Revista Colombiana de Biotecnología 21, no. 1 (January 1, 2019): 101–12. http://dx.doi.org/10.15446/rev.colomb.biote.v21n1.80248.

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Protoplasts are microbial or vegetable cells lacking a cell wall. These can be obtained from microalgae by an enzymatic hydrolysis process in the presence of an osmotic stabilizer. In general, protoplasts are experimentally useful in physiological, geneticand bio-chemical studies, so their acquisition and fusion will continue to be an active research area in modern biotechnology. The fusion of protoplasts in microalgae constitutes a tool for strain improvement because it allows both intra and interspecific genetic recombina-tion, resulting in organisms with new or improved characteristics of industrial interest. In this review we briefly describe themethod-ology for obtaining protoplasts, as well as fusion methods and the main applications of microalgal platforms.

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Puri, Munish. "Algal biotechnology for pursuing omega-3 fatty acid (bioactive) production." Microbiology Australia 38, no. 2 (2017): 85. http://dx.doi.org/10.1071/ma17036.

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Algae are spread in diversified ecosystems that include marine, freshwater, desert and hot springs and even snow and ice environments. Algae are classified as multicellular large sea weeds (macroalgae) or unicellular microalgae. Macroalgae are targeted for mining of natural biologically active components, which include proteins, linear peptides, cyclic peptides, and amino acids1. Recently, microalgae have been exploited for the production of high-value compounds such as lipids (omega-3 fatty acids), enzymes, polymers, toxins, antioxidants, and pigments (carotenoids)2. Thus, algal biotechnology is defined as ‘the technology developed using algae (macro or micro) to make or modify bioactive compounds, or products (nutritional supplements, fine chemicals) and renewable fuels for specific use’.

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de Morais, Michele Greque, Bruna da Silva Vaz, Etiele Greque de Morais, and Jorge Alberto Vieira Costa. "Biologically Active Metabolites Synthesized by Microalgae." BioMed Research International 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/835761.

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Microalgae are microorganisms that have different morphological, physiological, and genetic traits that confer the ability to produce different biologically active metabolites. Microalgal biotechnology has become a subject of study for various fields, due to the varied bioproducts that can be obtained from these microorganisms. When microalgal cultivation processes are better understood, microalgae can become an environmentally friendly and economically viable source of compounds of interest, because production can be optimized in a controlled culture. The bioactive compounds derived from microalgae have anti-inflammatory, antimicrobial, and antioxidant activities, among others. Furthermore, these microorganisms have the ability to promote health and reduce the risk of the development of degenerative diseases. In this context, the aim of this review is to discuss bioactive metabolites produced by microalgae for possible applications in the life sciences.

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Wijffels, René H. "Potential of sponges and microalgae for marine biotechnology." Trends in Biotechnology 26, no. 1 (January 2008): 26–31. http://dx.doi.org/10.1016/j.tibtech.2007.10.002.

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Juang, Yi-Je, and Jo-Shu Chang. "Applications of microfluidics in microalgae biotechnology: A review." Biotechnology Journal 11, no. 3 (January 25, 2016): 327–35. http://dx.doi.org/10.1002/biot.201500278.

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Nemtseva, N. V., O. A. Gogoleva, and M. E. Ignatenko. "BIOMEDICAL POTENTIAL OF ALGO-BACTERIAL SYMBIOSES." Journal of microbiology epidemiology immunobiology, no. 4 (August 28, 2018): 82–87. http://dx.doi.org/10.36233/0372-9311-2018-4-82-87.

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The analysis of the latest published works on the interactions between microalgae and bacteria is presented. Microalgae as a result of multimillion evolution can interact with each other and with another microorganisms. Interactions between algae and bacteria demonstrate a variety of communication from mutualism to parasitism. They can significantly affect the maintenance of vital activity, determines the direction vector, ensure the integrity of ecosystems. In modern society the attention of researches to algae-bacterial symbiosis increases as a biomass producer and as biologically active compounds. The development of green biotechnology is aimed at creating new directions for the use of algae-bacterial interactions. The analyzes materials testify to the high fundamental and applied potential of symbiosis microalgae with bacteria for biology and medicine.

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Soreanu, Gabriela, Igor Cretescu, Mariana Diaconu, Maria Ignat, Valeria Harabagiu, Corneliu Cojocaru, and Petrisor Samoila. "Multi-function biosystem based on Arthrospira Platensis for space applications." SIMI 2019, Abstract Book, SIMI 2019 (September 20, 2019): 77–83. http://dx.doi.org/10.21698/simi.2019.fp10.

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This study presents an introduction in air revitalisation practice by using a microalgae-based biosystem. Although the study is developed in the light of space applications, it opens new horizons for implementing such systems for terrestrial applications as well, where biotechnologies for addressing climate change and other issues associated with air pollution is in high demand. The experiments have been performed at laboratory-scale by using Arthrospira (spirulina) platensis as microalgae model. Influence of culture and illumination conditions, as well initial gas composition and other factors/techniques such culture filtration on the biosystem performance were investigated and the relevant results are presented and discussed. Based on the actual findings, future research needs are addressed.

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Silva, Teresa Lopes da, Patrícia Moniz, Carla Silva, and Alberto Reis. "The Role of Heterotrophic Microalgae in Waste Conversion to Biofuels and Bioproducts." Processes 9, no. 7 (June 23, 2021): 1090. http://dx.doi.org/10.3390/pr9071090.

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In the last few decades, microalgae have attracted attention from the scientific community worldwide, being considered a promising feedstock for renewable energy production, as well as for a wide range of high value-added products such as pigments and poly-unsaturated fatty acids for pharmaceutical, nutraceutical, food, and cosmetic markets. Despite the investments in microalgae biotechnology to date, the major obstacle to its wide commercialization is the high cost of microalgal biomass production and expensive product extraction steps. One way to reduce the microalgae production costs is the use of low-cost feedstock for microalgae production. Some wastes contain organic and inorganic components that may serve as nutrients for algal growth, decreasing the culture media cost and, thus, the overall process costs. Most of the research studies on microalgae waste treatment use autotrophic and mixotrophic microalgae growth. Research on heterotrophic microalgae to treat wastes is still scarce, although this cultivation mode shows several benefits over the others, such as higher organic carbon load tolerance, intracellular products production, and stability in production all year round, regardless of the location and climate. In this review article, the use of heterotrophic microalgae to simultaneously treat wastes and produce high value-added bioproducts and biofuels will be discussed, critically analyzing the most recent research done in this area so far and envisioning the use of this approach to a commercial scale in the near future.

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Nouemssi, Serge Basile, Manel Ghribi, Rémy Beauchemin, Fatma Meddeb-Mouelhi, Hugo Germain, and Isabel Desgagné-Penix. "Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii." Life 10, no. 9 (September 10, 2020): 186. http://dx.doi.org/10.3390/life10090186.

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Microalgae biotechnologies are rapidly developing into new commercial settings. Several high value products already exist on the market, and biotechnological development is focused on genetic engineering of microalgae to open up future economic opportunities for food, fuel and pharmacological production. Colony-polymerase chain reaction (colony-PCR or cPCR) is a critical method for screening genetically transformed microalgae cells. However, the ability to rapidly screen thousands of transformants using the current colony-PCR method, becomes a very laborious and time-consuming process. Herein, the non-homologous transformation of Chlamydomonas reinhardtii using the electroporation and glass beads methods generated more than seven thousand transformants. In order to manage this impressive number of clones efficiently, we developed a high-throughput screening (HTS) cPCR method to rapidly maximize the detection and selection of positively transformed clones. For this, we optimized the Chlamydomonas transformed cell layout on the culture media to improve genomic DNA extraction and cPCR in 96-well plate. The application of this optimized HTS cPCR method offers a rapid, less expensive and reliable method for the detection and selection of microalgae transformants. Our method, which saves up to 80% of the experimental time, holds promise for evaluating genetically transformed cells and selection for microalgae-based biotechnological applications such as synthetic biology and metabolic engineering.

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Dehghani, Jaber, Khosro Adibkia, Ali Movafeghi, Mohammad M. Pourseif, and Yadollah Omidi. "Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2." BioImpacts 10, no. 4 (July 13, 2020): 259–68. http://dx.doi.org/10.34172/bi.2020.33.

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Introduction: Attributable to some critical features especially the similarity of the protein synthesis machinery between humans and microalgae, these microorganisms can be utilized for the expression of many recombinant proteins. However, low and unstable gene expression levels prevent the further development of microalgae biotechnology towards protein production. Methods: Here, we designed a novel "Gained Agrobacterium-2A plasmid for microalgae expression" (named GAME plasmid) for the production of the human interleukin-2 using three model microalgae, including Chlamydomonas reinhardtii, Chlorella vulgaris, and Dunaliella salina. The GAME plasmid harbors a native chimeric hsp70/Int-1/rbcS2 promoter, the microalgae specific Kozak sequence, a novel hybrid 2A peptide, and Int-1 and Int-3 of the rbcS2 gene in its expression cassette. Results: The obtained data confirmed that the GAME plasmid can transform the microalgae with high transformation frequency. Molecular and proteomic analyses revealed the stable and robust production of the hIL-2 by the GAME plasmid in the microalgae. According to the densimetric analysis, the microalgae can accumulate the produced protein about 0.94% of the total soluble protein content. The ELISA data confirmed that the produced hIL-2 possesses the same conformation pattern with the acceptable biological activity found naturally in humans. Conclusion: Most therapeutic proteins need post-translational modifications for their correct conformation, biological function, and half-life. Accordingly, microalgae could be considered as a cost-effective and more powerful platform for the production of a wide range of recombinant proteins such as antibodies, enzymes, hormones, and vaccines.

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Hlavova, Monika, Zoltan Turoczy, and Katerina Bisova. "Improving microalgae for biotechnology — From genetics to synthetic biology." Biotechnology Advances 33, no. 6 (November 2015): 1194–203. http://dx.doi.org/10.1016/j.biotechadv.2015.01.009.

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Lu, Yandu, and Jian Xu. "Phytohormones in microalgae: a new opportunity for microalgal biotechnology?" Trends in Plant Science 20, no. 5 (May 2015): 273–82. http://dx.doi.org/10.1016/j.tplants.2015.01.006.

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Wijffels, René H., Olaf Kruse, and Klaas J. Hellingwerf. "Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae." Current Opinion in Biotechnology 24, no. 3 (June 2013): 405–13. http://dx.doi.org/10.1016/j.copbio.2013.04.004.

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Vieira Costa, Jorge Alberto, Ana Luiza Machado Terra, Nidria Dias Cruz, Igor Severo Gonçalves, Juliana Botelho Moreira, Suelen Goettems Kuntzler, and Michele Greque de Morais. "Microalgae Cultivation and Industrial Waste: New Biotechnologies for Obtaining Silver Nanoparticles." Mini-Reviews in Organic Chemistry 16, no. 4 (March 19, 2019): 369–76. http://dx.doi.org/10.2174/1570193x15666180626141922.

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Industrial effluents containing heavy metals can have harmful effects on organisms and the ecosystem. Silver is a waste from textile, galvanic and photographic industries, and when released into the environment, it can harm human health and cause biological modification. Removal of metals, such as silver, has been traditionally carried out using physicochemical methods that produce a high concentration of sludge and expend a significant amount of energy. Researchers are seeking innovative technologies for more efficient removal of silver or for using this heavy metal to obtain new products. The use of microalgae is a promising alternative to traditional remediation methods because several species can absorb and assimilate heavy metals. When exposed to toxic substances, microalgae excrete molecules in the medium that induce the reduction of silver particles to nanoparticles. Biosynthesized silver nanoparticles (AgNPs) can be used in medicine, food packaging, the production of cosmetics and pharmaceuticals, civil engineering, sensors and water purification. Thus, microalgal biosynthesis of metal nanoparticles has the capacity to bioremediate metals and subsequently convert them into non-toxic forms in the cell. In this context, this review addresses the use of microalgal biotechnology for industrial waste remediation of silver, which includes the simultaneous biosynthesis of AgNPs. We also discuss the potential applications of these nanoparticles.

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Shetty, Prateek, Margaret Mukami Gitau, and Gergely Maróti. "Salinity Stress Responses and Adaptation Mechanisms in Eukaryotic Green Microalgae." Cells 8, no. 12 (December 17, 2019): 1657. http://dx.doi.org/10.3390/cells8121657.

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High salinity is a challenging environmental stress for organisms to overcome. Unicellular photosynthetic microalgae are especially vulnerable as they have to grapple not only with ionic imbalance and osmotic stress but also with the generated reactive oxygen species (ROS) interfering with photosynthesis. This review attempts to compare and contrast mechanisms that algae, particularly the eukaryotic Chlamydomonas microalgae, exhibit in order to immediately respond to harsh conditions caused by high salinity. The review also collates adaptation mechanisms of freshwater algae strains under persistent high salt conditions. Understanding both short-term and long-term algal responses to high salinity is integral to further fundamental research in algal biology and biotechnology.

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Burczyk, Jan, Maria Zych, Nikolaos E. Ioannidis, and Kiriakos Kotzabasis. "Polyamines in Cell Walls of Chlorococcalean Microalgae." Zeitschrift für Naturforschung C 69, no. 1-2 (February 1, 2014): 75–80. http://dx.doi.org/10.5560/znc.2012-0215.

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Biotechnology of microalgae represents a very attractive alternative as a source of energy and substances of high value when compared with plant cultivation. Cell walls of green microalgae have an extraordinary chemical and mechanical resistance and may impede some steps in the biotechnological/industrial exploitation of algae. The aim of the present contribution was to check the presence of polyamines in the cell walls of chlorococcalean green microalgae. Polyamines are nitrogenous compounds synthesized normally in cells and may affect the properties of the cell wall. Our work included strains either forming or not forming the polymer algaenan, allowing us to conclude that algaenan is not a prerequisite for the presence of polyamines in the cell walls. Polyamines were detected in isolated cell walls of Scenedesmus obliquus, Chlorella fusca, Chlorella saccharophila, and Chlorella vulgaris. Their concentration in isolated cell walls ranged between 0.4 and 8.4 nmol/mg dry weight.

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Sangapillai, Karthikeyan, and Thirumarimurugan Marimuthu. "Isolation and selection of growth medium for freshwater microalgae Asterarcys quadricellulare for maximum biomass production." Water Science and Technology 80, no. 11 (December 1, 2019): 2027–36. http://dx.doi.org/10.2166/wst.2020.015.

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Abstract The use of microalgae biomass as a suitable alternative feedstock for biofuel production has been promoted in the field of green biotechnology. In this present study, the microalgae were isolated from freshwater samples. The predominant strain was screened from the samples and grown in four different growth media, including modified Bold's Basal Medium (BBM), modified CFTRI medium, BG11 medium and CHU medium, to find the suitable growth medium to enrich biomass production. In total three microalgae colonies were identified based on their colony morphology microscopically by using a light microscope. The predominant strain was confirmed as Asterarcys quadricellulare using 18S rRNA sequencing. The growth of microalgae was investigated based on parameters like dry weight, pigment composition such as chlorophyll a, chlorophyll b, carotenoid and lipid content in the microalgae. Among the four different media, modified BBM medium showed maximum dry weight (1.44 ± 0.015 g/L), chlorophyll a (23.07 ± 0.049 mg/L), chlorophyll b (16.76 ± 0.010 mg/L), carotenoid (8.92 ± 0.031 mg/L) and lipid content (375 ± 0.020 mg/L) on the 25th day of culture. The gas chromatography mass spectrometry (GC/MS) analysis showed the presence of major fatty acids stearic acid, palmitic acid and oleyl alcohol in the microalgae. Therefore the high lipid content and fatty acid profiles of Asterarcys quadricellulare are becoming a promising suitable strain for biofuel production with modified BBM medium.

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Vargas-Perez, Magda, Gerardo Sierra-García, Hugo Luna Olvera, Abelardo Chavez-Montes, and Azucena Gonzalez-Horta. "Impact of Melittin on Microalgae Cell Wall: A Monolayer Study." Natural Product Communications 13, no. 8 (August 2018): 1934578X1801300. http://dx.doi.org/10.1177/1934578x1801300822.

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The cell wall of microalgae presents a formidable barrier necessary for survival in aquatic environments. Unfortunately, this barrier affects certain processes of interest in algal biotechnology such as oil extraction. Thus, assessing the impact of lytic peptides or enzymes on algal cell wall degradation is a critical first step to utilizing algal biomass more efficiently. Galactolipids are the main structural component of plant chloroplastic membranes and blue-green algae cell membranes. The predominant lipids in this class are monogalactosyl-diacylglycerol (MGDG) and digalactosyl-diacylglycerol (DGDG). Here using de Langmuir monolayer technique, we have demonstrated that melittin, a lytic peptide, has an intrinsic propensity to interact and perturb interfacial monolayers made of MGDG or DGDG that mimic microalgae cell wall.

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Abt, Vinzenz, Fabian Gringel, Arum Han, Peter Neubauer, and Mario Birkholz. "Separation, Characterization, and Handling of Microalgae by Dielectrophoresis." Microorganisms 8, no. 4 (April 9, 2020): 540. http://dx.doi.org/10.3390/microorganisms8040540.

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Microalgae biotechnology has a high potential for sustainable bioproduction of diverse high-value biomolecules. Some of the main bottlenecks in cell-based bioproduction, and more specifically in microalgae-based bioproduction, are due to insufficient methods for rapid and efficient cell characterization, which contributes to having only a few industrially established microalgal species in commercial use. Dielectrophoresis-based microfluidic devices have been long established as promising tools for label-free handling, characterization, and separation of broad ranges of cells. The technique is based on differences in dielectric properties and sizes, which results in different degrees of cell movement under an applied inhomogeneous electrical field. The method has also earned interest for separating microalgae based on their intrinsic properties, since their dielectric properties may significantly change during bioproduction, in particular for lipid-producing species. Here, we provide a comprehensive review of dielectrophoresis-based microfluidic devices that are used for handling, characterization, and separation of microalgae. Additionally, we provide a perspective on related areas of research in cell-based bioproduction that can benefit from dielectrophoresis-based microdevices. This work provides key information that will be useful for microalgae researchers to decide whether dielectrophoresis and which method is most suitable for their particular application.

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Mendes, Leonardo Brantes Bacellar, Carolina Vieira Viegas, Rafael Richard Joao, and Ronaldo Bernardo da Silva. "Microalgae Production: A Sustainable Alternative for a Low-carbon Economy Transition." Open Microalgae Biotechnology 1, no. 1 (August 24, 2021): 1–7. http://dx.doi.org/10.2174/2666395302101010001.

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The production of microalgae on a commercial scale began in the 1970s. From this time until today it has consolidated itself as an alternative for human consumption and animal feed, mainly through aquaculture (carcinoculture, oyster farming, and fish farming). Currently, most of the micro-algal biomass that has been produced in photoautotrophic systems for human consumption comes from four main genera (Chlorella, Arthrospira, Dunaliella, and Haematococcus). Recent advances allowed Nannochloropsis and Euglena cultivation in open ponds for feed and fuels. Although the initiatives mentioned represent the success of the scale-up for microalgae production, there are challenges to be overcome for the use of the vast set of existing microalgae species. The promising future of the industry involved in large scale production of microalgae is supported by its characteristic that is clearly sustainable from an ecological point of view and in the transition proposal to a low carbon economy that has been intensified in response to the effects caused by the progressive release of CO2 in the atmosphere. Innovative applications from microalgae biotechnology are being developed every year. In this context, there have been several research and development initiatives over the past decade aimed at obtaining advanced fuels making full use of micro-algal biomass.

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Mahendran, Manishaa Sri, Sinouvassane Djearamane, Ling Shing Wong, Govindaraju Kasivelu, and Anto Cordelia Tanislaus Antony Dhanapal. "ANTIVIRAL PROPERTIES OF MICROALGAE AND CYANOBACTERIA." Journal of Experimental Biology and Agricultural Sciences 9, Spl-1- GCSGD_2020 (March 25, 2021): S43—S48. http://dx.doi.org/10.18006/2021.9(spl-1-gcsgd_2020).s43.s48.

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The recent outbreak of Corona Virus Disease (COVID-19) and the surge in accelerating the development of a vaccine to fight against the SARS-CoV-2 virus has imposed greater challenges to humanity worldwide. There is lack of research into the production of effective vaccines and methods of treatment against viral infections. As of now, strategies encompassing antiviral drugs and corticosteroids alongside mechanical respiratory treatment are in practice as frontline treatments. Though studies have reported that microalgae possess antiviral properties, only a few cases have presented the existence of antiviral compounds such as algal polysaccharides, lectins, aggluttinins, scytovirin, algal lipids such as sulfoquinovosyldiacylglycerol (SQDG), monogalactosyldiacylglycerides (MGDG) and digalactosyldiacylglycerides (DGDG), and algal biopigments especially chlorophyll analogues, marennine, phycobiliproteins, phycocyanin, phycoerythrin and allophycocyanin that are derived from marine and freshwater microalgae. Given the chemodiversity of bioactive compounds from microalgae and the present scenario, algal biotechnology is seen as a prospective source of antiviral and anti-inflammatory compounds that can be used to develop antiviral agents. Microalgae with potential as antivirals and microalgae derived functional compounds to treat viral diseases are summarized and can be used as a reference in developing algae-derived antivirals to treat SARS-CoV-2 and other similar viruses.

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Lafarga, Tomás, Carlo Pieroni, Giuliana D’Imporzano, Lorenzo Maggioni, Fabrizio Adani, and Gabriel Acién. "Consumer Attitudes towards Microalgae Production and Microalgae-Based Agricultural Products: The Cases of Almería (Spain) and Livorno (Italy)." ChemEngineering 5, no. 2 (May 28, 2021): 27. http://dx.doi.org/10.3390/chemengineering5020027.

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The production of microalgal biomass and products derived thereof for a wide variety of applications is a hot research topic, with the number of facilities being built and products and biologically active molecules launched into the market increasing every year. The aim of the current study was to identify the attitudes of citizens in Almería (Spain) and Livorno (Italy) towards the construction of a microalgae production plant and a biorefinery in their cities and also their opinions about the microalgae-based products that could be produced. Overall, in Almería (Spain), a NIMBY (not in my back yard) attitude towards the construction of a microalgal production facility and especially towards a microalgal biorefinery was observed, despite the strong microalgal industry in the region and the higher knowledge of citizens about microalgae. In both locations, but especially in Livorno (Italy), microalgae-based biostimulants, biofertilisers, and aquafeeds were well accepted. Proximity was the main factor affecting the acceptance of a microalgae producing facility. Consumer knowledge about microalgal biotechnology and the health and environmental benefits of this valuable raw material are scarce, and opinions are based on drivers other than knowledge. After gaining more knowledge about microalgal biorefineries, most of the responses in Almería (47%) and Livorno (61%) were more positive.

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Lopes da Silva, Teresa, Patrícia Moniz, Carla Silva, and Alberto Reis. "The Dark Side of Microalgae Biotechnology: A Heterotrophic Biorefinery Platform Directed to ω-3 Rich Lipid Production." Microorganisms 7, no. 12 (December 10, 2019): 670. http://dx.doi.org/10.3390/microorganisms7120670.

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Microbial oils have been considered a renewable feedstock for bioenergy not competing with food crops for arable land, freshwater and biodiverse natural landscapes. Microalgal oils may also have other purposes (niche markets) besides biofuels production such as pharmaceutical, nutraceutical, cosmetic and food industries. The polyunsaturated fatty acids (PUFAs) obtained from oleaginous microalgae show benefits over other PUFAs sources such as fish oils, being odorless, and non-dependent on fish stocks. Heterotrophic microalgae can use low-cost substrates such as organic wastes/residues containing carbon, simultaneously producing PUFAs together with other lipids that can be further converted into bioenergy, for combined heat and power (CHP), or liquid biofuels, to be integrated in the transportation system. This review analyses the different strategies that have been recently used to cultivate and further process heterotrophic microalgae for lipids, with emphasis on omega-3 rich compounds. It also highlights the importance of studying an integrated process approach based on the use of low-cost substrates associated to the microalgal biomass biorefinery, identifying the best sustainability methodology to be applied to the whole integrated system.

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Tharek, Ameerah, Shaza Eva Mohamad, Koji Iwamoto, Iwane Suzuki, Hirofumi Hara, Rozzeta Dolah, Shinji Yoshizaki, Haryati Jamaluddin, Madihah Md Salleh, and Adibah Yahya. "Enhanced astaxanthin production by oxidative stress using methyl viologen as a reactive oxygen species (ROS) reagent in green microalgae Coelastrum sp." Indonesian Journal of Biotechnology 25, no. 2 (November 10, 2020): 95. http://dx.doi.org/10.22146/ijbiotech.54092.

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Microalgae are known to be a potential resource of high-value metabolites that can be used in the growing field of biotechnology. These metabolites constitute valuable compounds with a wide range of applications that strongly enhance a bio-based economy. Among these metabolites, astaxanthin is considered the most important secondary metabolite, having superior antioxidant properties. For commercial feasibility, microalgae with enhanced astaxanthin production need to be developed. In this study, the tropical green microalgae strain, Coelastrum sp., isolated from the environment in Malaysia, was incubated with methyl viologen, a reactive oxygen species (ROS) reagent that generates superoxide anion radicals (O2-) as an enhancer to improve the accumulation of astaxanthin. The effect of different concentrations of methyl viologen on astaxanthin accumulation was investigated. The results suggested that the supplementation of methyl viologen at low concentration (0.001 mM) was successfully used as a ROS reagent in facilitating and thereby increasing the production of astaxanthin in Coelastrum sp. at a rate 1.3 times higher than in the control.

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Alonso, Mercedes, Fátima C. Lago, Juan M. Vieites, and Montserrat Espiñeira. "Molecular characterization of microalgae used in aquaculture with biotechnology potential." Aquaculture International 20, no. 5 (February 14, 2012): 847–57. http://dx.doi.org/10.1007/s10499-012-9506-8.

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Cheregi, Otilia, Susanne Ekendahl, Johan Engelbrektsson, Niklas Strömberg, Anna Godhe, and Cornelia Spetea. "Microalgae biotechnology in Nordic countries – the potential of local strains." Physiologia Plantarum 166, no. 1 (March 25, 2019): 438–50. http://dx.doi.org/10.1111/ppl.12951.

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Zhu, Lian Dong, Erkki Hiltunen, and Josu Takala. "Microalgal Biofuels Beat the First and Second Generation Biofuels." Applied Mechanics and Materials 197 (September 2012): 760–63. http://dx.doi.org/10.4028/www.scientific.net/amm.197.760.

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Recently biofuels derived from biomass have received increased concerns in an attempt to search for sustainable development. The first and second generation biofuels are unsustainable since the growth of these food or non-food crops for biofuel generation will compete for limited arable farmlands, thus increasing the risks on food availability. Microalgal biofuels, known as the third generation biofuels, have the potential for sustainable production in an economically effective manner. The advantages of microalgae as a biofuel feedstock are many, for instance, high photosynthesis efficiency, high oil content and noncompetition with food crop production on farmlands. Microalgae can be employed for the production of biodiesel, bioethanol, biogas, biohydrogen, among others. The integrated biorefinery approach has huge potential to greatly improve the economics of biofuel production from microalgae. However, the production of microalgal biofuels is still at pre-commercial stages since it is expensive to produce substantial amount of biofuels at a large scale. Despite this, microalgae are still the most promising and best feedstock available for the biofuels. Biotechnology advances including genetic and metabolic engineering, well-funded R&D researches and policy support can make microalgal biofuels have a bright future.

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Kawano, Shigeyuki, and Masanobu Kawachi. "Various attractions of microalgae: systematics, evolution, genome to morphology, algal biotechnology." PLANT MORPHOLOGY 29, no. 1 (2017): 39–40. http://dx.doi.org/10.5685/plmorphol.29.39.

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Araújo, Fabíola Ornellas de, Reinaldo Giudici, and João José Martins Simões de Sousa. "CULTIVATION OF THE MICROALGAE CHLORELLA PYRENOIDOSA USING THE PROCESSES OF BIOTECHNOLOGY." Revista Eletrônica Acervo Científico 2 (March 26, 2019): 121. http://dx.doi.org/10.25248/reac.e121.2019.

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The results obtained here, show that the use of Chlorella pyrenoidosa microalgae and biotechnology, using the discontinuous process, presented satisfactory results. With this, the study of the microalga Chlorella sp. has proved to be important because it has a wealth of proteins, carbohydrates, amino acids, fatty acids, carotenoids, vitamins and minerals in its constitution, which may represent commercial importance. This research revealed the best results for obtaining a lipoprotein-rich biomass, taking into account three different culture media, calculations of cell concentration, cell productivity, to the content (%) of protein, lipid, carbohydrate and ash present in the microalgal biomass.

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Moraes, Iracema De Oliveira, Regina De Oliveira Moraes Arruda, Rodrigo De Oliveira Moraes, and Maria Josiane Conti Moraes. "HUMAN RESOURCES TRAINING IN BIOTECHNOLOGY: MICROALGAE FOR BIOFUEL AND WASTEWATER TREATMENT." Revista Geociências - UNG-Ser 18, no. 1 (December 4, 2019): 5. http://dx.doi.org/10.33947/1981-7428-v18n1-3976.

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The most promising and innovative alternative to biodiesel production is presented by the algae (micro and macroalgae), which have been classified by scientists as a source of third generation biofuels. The large-scale production of biodiesel from microalgae and macroalgae bioethanol production will happen much faster than you think. It is believed that its full commercialization is possible within a few years, and with a competitive price compared to diesel produced from petroleum, the same occurring for bioethanol. The use of seaweed as a feedstock for the production of biofuels has been seen as a less environmentally impactful as the biomass produced on the continent and its potential is very high. Several groups in Latin America (Brazil, of course) are studying micro and macroalgae not only for biofuels production (biodiesel, bioethanol, biohydrogen) but also to do wastewater treatment. This paper will discuss the presentations done in four annual courses (2010, 2011, 2012, 2013) promoted by the Biotechnology Brazilian Argentine Center, BBAC, and offered to fellowships (sixteen per year), from Brazil, Argentina, Colombia, Paraguay and Uruguay. Arthrospira (Spirulina) platensis was chosen as a cyanobacteria model in the courses, due to its feasibility of cultivation, many publications about the subject and the existence of strains isolated in Brazil.

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Rinanti, Astri, and Ronny Purwadi. "Bioflocculation Activity in Harvesting System: A Biotechnology Approach for Microalgae Biomass." Aceh International Journal of Science and Technology 7, no. 1 (April 21, 2018): 69–76. http://dx.doi.org/10.13170/aijst.7.1.8860.

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A study on Ankistrodesmus sp.—a freshwater green microalgae species—as a bioflocculant based on its physicochemical properties and flocculation rate has been carried out. The molecular identification via 16S rDNA showed 99% resemblance of this green microalga to the Ankistrodesmus fumigatus strain. The optimum batch culture condition for the bioflocculant production was initiated by 10% inoculum (v/v). The low-concentrated bioflocculant of 10% (v/v) is considered as thermostable with a high flocculation rate to harvest the biomass of Chlorella sp. at a pH range of 5 to 9. The source of molasses, the mixture of yeast extract were used as the optimum sources of carbon and Ammonium sulfate were used as the optimum sources of nitrogen in the growth medium. Ankistrodesmus sp. bioflocculant has a high flocculation efficiency over a wide range of pH (5–9) with a low dose requirement of 10% v/v at 25°C. Hence, it is immensely competitive to promote the economic viability of the production process. Accordingly, Ankistrodesmus sp. bioflocculant has a high potential to be applied on an industrial scale in tropical regions as it does not require additional production cost.

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Khozin-Goldberg, Inna, Umidjon Iskandarov, and Zvi Cohen. "LC-PUFA from photosynthetic microalgae: occurrence, biosynthesis, and prospects in biotechnology." Applied Microbiology and Biotechnology 91, no. 4 (July 1, 2011): 905–15. http://dx.doi.org/10.1007/s00253-011-3441-x.

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Schulze, Peter S. C., Christopher J. Hulatt, Daniela Morales-Sánchez, René H. Wijffels, and Viswanath Kiron. "Fatty acids and proteins from marine cold adapted microalgae for biotechnology." Algal Research 42 (September 2019): 101604. http://dx.doi.org/10.1016/j.algal.2019.101604.

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Silkina, Alla, Naomi E. Ginnever, Fleuriane Fernandes, and Claudio Fuentes-Grünewald. "Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform." Energies 12, no. 14 (July 19, 2019): 2772. http://dx.doi.org/10.3390/en12142772.

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Municipal and agricultural waste treatment is one of the key elements of reducing environmental impact with direct effects on the economy and society. Algal technology has been tested to enable effective recycling and valorisation of wastewater nutrients including carbon, nitrogen and phosphorus. An integrated evaluation and optimisation of the sustainability of an algal bio-refinery, including mass and energy balances, carbon, water and nutrient use and impact analysis, was assessed. A bio-refinery approach of waste remediation using algal cultivation was developed at Swansea University, focusing on nutrient recovery via algal biomass exploitation in pilot facilities. Mass cultivation (up to 1.5 m3) was developed with 99% of nitrogen and phosphorus uptake by microalgal cultures. Nannochloropsis oceanica was used as a biological model and grown on three waste sources. The compounds obtained from the biomass were evaluated for animal feed and as a potential source of energy. The bioremediation through algal biotechnology was examined and compared to alternative nutrient recovery passive and active methods in order to know the most efficient way of excess nutrient management. Conclusions emphasise the high potential of algal biotechnology for waste remediation and nutrients recovery, despite the need for further development and scalable applications of this new technology.

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Pomponi, Shirley A., Daniel G. Baden, and Yonathan Zohar. "Marine Biotechnology: Realizing the Potential." Marine Technology Society Journal 41, no. 3 (September 1, 2007): 24–31. http://dx.doi.org/10.4031/002533207787442132.

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Marine biotechnology is an applied science, the goal of which is to develop goods and services from marine organisms and processes. The new wave of marine biotechnology research began in the early 1980s and includes some significant success stories. A new drug to manage pain is commercially available, and a new cancer drug has been recommended for approval, the first from a fish-eating snail and the second from a mangrove tunicate. Enzymes from hydrothermal vent microbes are routinely used in PCR reactions, and marine-derived molecular probes are helping understand the molecular basis of disease processes. Advances in aquaculture biotechnology have resulted in more efficient production of finfish and shellfish for human consumption, and polyunsaturated fatty acids from marine microalgae are used as nutritional supplements for adults and infants. Rapid diagnostic tools have been developed to monitor toxins in the environment and in seafood, and genetic fingerprinting techniques are helping to control illegal trade of threatened marine species. In the future, multidisciplinary programs in oceans and human health should focus not only on microbial pathogens and harmful algal bloom toxins but also on discovery of new chemicals to prevent or treat human diseases. And the development of biological and biochemical sensors to detect pathogens, contaminants, and toxins and to monitor human and environmental health indicators in the marine environment should be a very high priority in the establishment of U.S. coastal ocean observing systems.

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Potocki, Leszek, Bernadetta Oklejewicz, Ewelina Kuna, Ewa Szpyrka, Magdalena Duda, and Janusz Zuczek. "Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species." Molecules 26, no. 13 (July 1, 2021): 4038. http://dx.doi.org/10.3390/molecules26134038.

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As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades. Microalgae can be used for biomass production and to obtain biotechnologically important products. Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp. as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism. The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620). The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053. The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement. The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.

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Castiglia, Daniela, Simone Landi, and Sergio Esposito. "Advanced Applications for Protein and Compounds from Microalgae." Plants 10, no. 8 (August 16, 2021): 1686. http://dx.doi.org/10.3390/plants10081686.

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Algal species still show unrevealed and unexplored potentiality for the identification of new compounds. Photosynthetic organisms represent a valuable resource to exploit and sustain the urgent need of sustainable and green technologies. Particularly, unconventional organisms from extreme environments could hide properties to be employed in a wide range of biotechnology applications, due to their peculiar alleles, proteins, and molecules. In this review we report a detailed dissection about the latest and advanced applications of protein derived from algae. Furthermore, the innovative use of modified algae as bio-reactors to generate proteins or bioactive compounds was discussed. The latest progress about pharmaceutical applications, including the possibility to obtain drugs to counteract virus (as SARS-CoV-2) were also examined. The last paragraph will survey recent cases of the utilization of extremophiles as bio-factories for specific protein and molecule production.

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Journal articles on the topic 'Microalgues – Biotechnologie'

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