Academic literature on the topic 'Microarray immunoassay'

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Journal articles on the topic "Microarray immunoassay"

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Han, Dongsik, and Je-Kyun Park. "Microarray-integrated optoelectrofluidic immunoassay system." Biomicrofluidics 10, no. 3 (2016): 034106. http://dx.doi.org/10.1063/1.4950787.

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Uenoyama, Yuta, Atsushi Matsuda, Kazune Ohashi, et al. "Development and Evaluation of a Robust Sandwich Immunoassay System Detecting Serum WFA-Reactive IgA1 for Diagnosis of IgA Nephropathy." International Journal of Molecular Sciences 23, no. 9 (2022): 5165. http://dx.doi.org/10.3390/ijms23095165.

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Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an automated sandwich immunoassay system for detecting aberrant glycosylated IgA1, using Wisteria floribunda agglutinin (WFA) and anti-IgA1 monoclonal antibody. The diagnostic performance as an IgAN marker was evaluated. The usefulness of WFA for immunoassays was investigated by lectin microarray. A reliable standard for quantitative immunoassay measur
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Shao, Weiping, Zhimin Zhou, Isabelle Laroche, et al. "Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling." Journal of Biomedicine and Biotechnology 2003, no. 5 (2003): 299–307. http://dx.doi.org/10.1155/s1110724303209268.

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Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simulta
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Rafique, Saima, Farukh Kiyani, Sumbal Jawaid, et al. "Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum." BioMed Research International 2021 (July 15, 2021): 1–11. http://dx.doi.org/10.1155/2021/9916909.

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The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-n
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Zhang, Daxiao, Wei Dai, Huatian Hu, et al. "Controlling the immobilization process of an optically enhanced protein microarray for highly reproducible immunoassay." Nanoscale 13, no. 7 (2021): 4269–77. http://dx.doi.org/10.1039/d0nr08407g.

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Song, Yujing, Jingyang Zhao, Tao Cai, et al. "Machine learning-based cytokine microarray digital immunoassay analysis." Biosensors and Bioelectronics 180 (May 2021): 113088. http://dx.doi.org/10.1016/j.bios.2021.113088.

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Silzel, John W., Bibijana Cercek, Charles Dodson, Tsong Tsay, and Robert J. Obremski. "Mass-sensing, multianalyte microarray immunoassay with imaging detection." Clinical Chemistry 44, no. 9 (1998): 2036–43. http://dx.doi.org/10.1093/clinchem/44.9.2036.

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Abstract Miniaturization of ligand binding assays may reduce costs by decreasing reagent consumption, but it is less apparent that miniaturized assays can simultaneously exceed the sensitivity of macroscopic techniques by analyte “harvesting” to exploit the total analyte mass available in a sample. Capture reagents (avidin or antibodies) immobilized in 200-μm diameter zones are shown to substantially deplete analyte from a liquid sample during a 1–3-h incubation, and the assays that result sense the total analyte mass in a sample rather than its concentration. Detection of as few as 105 molecu
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Anderson, George P., and Chris R. Taitt. "Suspension Microarray Immunoassay Signal Amplification Using Multilayer Formation." Sensor Letters 6, no. 1 (2008): 213–18. http://dx.doi.org/10.1166/sl.2008.018.

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Päkkilä, Henna, Minna Ylihärsilä, Satu Lahtinen, et al. "Quantitative Multianalyte Microarray Immunoassay Utilizing Upconverting Phosphor Technology." Analytical Chemistry 84, no. 20 (2012): 8628–34. http://dx.doi.org/10.1021/ac301719p.

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Lee, Jonghwan, Jaeyeon Jung, Subeom Park, Jie Chen, Jaeyoo Choi, and Jinho Hyun. "Microarray of stimuli-responsive microbeads for duplexed immunoassay." BioChip Journal 5, no. 2 (2011): 158–64. http://dx.doi.org/10.1007/s13206-011-5209-x.

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Dissertations / Theses on the topic "Microarray immunoassay"

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Nambiar, Kate. "Bioinformatic analysis of peptide microarray immunoassay data for serological diagnosis of infectious diseases." Thesis, University of Brighton, 2017. https://research.brighton.ac.uk/en/studentTheses/de2be38a-5941-4bc5-adb9-1c200b07c193.

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Understanding antibody - antigen interactions occurring in infectious diseases is important in understanding aetiology, can help facilitate diagnosis, and could offer potential targets for vaccine or therapeutic antibody development. Peptide arrays – collections of short peptides immobilised on solid planar supports – offer a high throughput and highly parallel method of identifying immunogenic epitopes and relating patterns of antibody identification to clinical disease states. As technology advances, so the density and complexity of peptide arrays of becomes ever higher. Managing the large v
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Ericsson, Olle. "Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8475.

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Gagni, P. "DEVELOPMENT OF NOVEL HIGH PERFORMANCE PROTEIN MICROARRAYS FOR DIAGNOSTIC APPLICATIONS." Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/249493.

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Several application of protein microarray technology in diagnostics have been published and a limited number of protein microarrays is currently available on the In Vitro Diagnostics (IVD) market. Albeit several advantages, related to the miniaturization, the multiplexing capability and the possibility of integrating the immunoassays in biosensing devices, microarrays may still lack of specificity or sensitivity. To overcome these limitations and expand the use of protein microarray platform in diagnostics, the present PhD research aimed at developing innovative approaches to increase the assa
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Byström, Sanna. "Affinity assays for profiling disease-associated proteins in human plasma." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-202616.

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Affinity-based proteomics offers opportunities for the discovery and validation of disease-associated proteins in human body fluids. This thesis describes the use of antibody-based immunoassays for multiplexed analysis of proteins in human plasma, serum and cerebrospinal fluid (CSF). This high-throughput method was applied with the objective to identify proteins associated to clinical variables. The main work in this thesis was conducted within the diseases of multiple sclerosis and malignant melanoma, as well as mammographic density, a risk factor for breast cancer. The suspension bead array
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Paoli, Marine de. "Cancer de la vessie : sélection de biomarqueurs urinaires et développement d’un outil d’analyse multiparamétrique pour le diagnostic et la récidive des tumeurs urothéliales." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1158/document.

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Les travaux présentés dans cette thèse concernent le développement d'un outil d'analyse multiparamétrique pour la quantification de biomarqueurs urinaires du cancer de la vessie.La première partie des travaux de recherche a pour objectif la sélection de marqueurs pour le diagnostic et la récidive des tumeurs urothéliales. Une première étude a permis l'évaluation de la sélectivité de marqueurs candidats dans des échantillons urinaires de patients atteints de cancer de la vessie. Cinq des vingt marqueurs initiaux ont été sélectionnés pour leur performance diagnostique, définissant le Panel 1 : V
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Fernández, Arauzo Leticia. "Péptidos sintéticos del GB virus C. Aplicación en el diagnóstico de infección y en el diseño de potenciales agentes terapéuticos contra el VIH-1." Doctoral thesis, Universitat de Barcelona, 2012. http://hdl.handle.net/10803/482076.

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El GB virus C (GBV-C), también conocido como virus de la hepatitis G, es un virus humano no patogénico. Su infección es bastante frecuente, encontrándose incluso en personas sanas. Su prevalencia es mucho mayor en individuos considerados de riesgo, ya que comparte las vías de transmisión con otros virus, como por ejemplo con el virus de la hepatitis B, el virus de la hepatitis C o el virus de la inmunodeficiencia humana (VIH). Numerosos estudios asocian la coinfección del GBV-C y el VIH con una menor progresión de la enfermedad y con una mayor supervivencia de los pacientes una vez que el SI
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Kibat, Janek Norman [Verfasser], and Gerhard [Akademischer Betreuer] Winter. "Development of a protein microarray platform for the characterization of antibodies and quantitative immunoassays / Janek Norman Kibat ; Betreuer: Gerhard Winter." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1167683161/34.

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Drobin, Kimi. "Antibody-based bead arrays for high-throughput protein profiling in human plasma and serum." Licentiate thesis, KTH, Proteinvetenskap, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-225980.

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Affinity-based proteomics utilizes affinity binders to detect target proteins in a large-scale manner. This thesis describes a high-throughput method, which enables the search for biomarker candidates in human plasma and serum. A highly multiplexed antibody-based suspension bead array is created by coupling antibodies generated in the Human Protein Atlas project to color-coded beads. The beads are combined for parallel analysis of up to 384 analytes in patient and control samples. This provides data to compare protein levels from the different groups. In paper I osteoporosis patients are compa
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Lee, Kuo-Hoong, and 李國宏. "Microfluidic systems for SPR sensing using microarray immunoassay." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/08040366249125116195.

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碩士<br>國立成功大學<br>工程科學系碩博士班<br>94<br>This study reports a novel microfluidic chip integrated with arrayed immunoassay for SPR (Surface plasmon resonance) phase imaging of specific bio-samples. The SPR imaging system uses a surface-sensitive optical technique to detect two-dimensional spatial phase variation caused by antibodies absorbed on a sensing surface composed of highly-specific proteins films. The developed system has a high resolution and a high-throughput screening capability and has been successfully applied to the analysis of multiple bio-molecules without the need for additional labe
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Uddayasankar, Uvaraj. "Towards a Surface Microarray based Multiplexed Immunoassay on a Digital Microfluidics Platform." Thesis, 2010. http://hdl.handle.net/1807/25832.

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The use of digital microfluidics (DMF) for sample handling in a microarray immunoassay was investigated. A two plate DMF device was used, with the top plate being used for the immobilization of antibodies for a sandwich immunoassay. A patterning procedure was developed for the top plate to expose patches of glass that were chemically modified, using silane chemistry, to allow for the covalent immobilization of antibodies. For creating microarrays, a set of parallel microchannels were used for the high density patterning of proteins onto the functionalized surface of the top plate. This pattern
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Book chapters on the topic "Microarray immunoassay"

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Gimenez, Gustavo, Sara Benedé, and Jing Lin. "IgE Epitope Mapping Using Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3037-1_19.

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Lin, Jing, and Hugh A. Sampson. "IgE Epitope Mapping Using Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6925-8_14.

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Jambari, Nuzul N., XiaoWei Wang, and Marcos Alcocer. "Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6925-8_10.

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Oswald, Susanna, Richard Dietrich, Erwin Märtlbauer, Reinhard Niessner, and Dietmar Knopp. "Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6682-0_11.

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Quaranta, Patricia, Rocío Paz-González, Selva Riva-Mendoza, et al. "Optimization of Serum and Microarray Concentrations for a Semiquantitative Multiplex Indirect Immunoassay." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4595-6_6.

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Martínez-Botas, Javier, and Belén de la Hoz. "IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3037-1_18.

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Lisson, Maria, and Georg Erhardt. "Mapping of Epitopes Occurring in Bovine αs1-Casein Variants by Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3037-1_21.

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Martínez-Botas, Javier, Carlos Fernández-Lozano, Aida Vaquero-Rey, and Belén de la Hoz. "IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2732-7_16.

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Chu, F. W., P. R. Edwards, R. P. Ekins, H. Berger, P. Finckh, and F. Krause. "Microarray-Based Immunoassays." In ACS Symposium Series. American Chemical Society, 1997. http://dx.doi.org/10.1021/bk-1997-0657.ch014.

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Prabhakar, Uma, Edward Eirikis, Bruce E. Miller, and Hugh M. Davis. "Multiplexed Cytokine Sandwich Immunoassays Clinical Applications." In Microarrays in Clinical Diagnostics. Humana Press, 2005. http://dx.doi.org/10.1385/1-59259-923-0:223.

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Conference papers on the topic "Microarray immunoassay"

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Sigurdson, M., C. Meinhart, and D. Wang. "AC Electrokinetics for Biosensors." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-62013.

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We develop here tools for speeding up binding in a biosensor device through augmenting diffusive transport, applicable to immunoassays as well as DNA hybridization, and to a variety of formats, from microfluidic to microarray. AC electric fields generate the fluid motion through the well documented but unexploited phenomenon, Electrothermal Flow, where the circulating flow redirects or stirs the fluid, providing more binding opportunities between suspended and wall-immobilized molecules. Numerical simulations predict a factor of up to 8 increase in binding rate for an immunoassay under reasona
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Nichkova, Mikaela, Dosi Dosev, Shirley J. Gee, Bruce D. Hammock, and Ian M. Kennedy. "Microarray immunoassay for phenoxybenzoic acid using polymer-functionalized lanthanide oxide nanoparticles as fluorescent labels." In Optics East 2005, edited by M. Saif Islam and Achyut K. Dutta. SPIE, 2005. http://dx.doi.org/10.1117/12.631032.

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Bijlsma, D., E. Lukasik, M. Hausmann, et al. "AB1518 ANALYTICAL PERFORMANCE OF A NOVEL, FULLY AUTOMATED MULTIPLEXED MICROARRAY IMMUNOASSAY PROTOTYPE FOR THE SIMULTANEOUS DETECTION OF FIFTEEN AUTOANTIBODIES ASSOCIATED WITH CONNECTIVE TISSUE DISEASES." In EULAR 2024 European Congress of Rheumatology, 12-15 June. Vienna, Austria. BMJ Publishing Group Ltd and European League Against Rheumatism, 2024. http://dx.doi.org/10.1136/annrheumdis-2024-eular.5595.

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Auernhammer, A., M. Seidel, and C. Hu. "P4.6 - Kombinierung eines Wasser-Sensorsystems mit einem vollautomatischen indirekt kompetitiven Durchfluss-Microarray-Immunoassay als Frühwarn-System für vermehrtes Algenwachstum und Freisetzung von Algentoxinen in Oberflächengewässern." In 15. Dresdner Sensor-Symposium 2021. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2021. http://dx.doi.org/10.5162/15dss2021/p4.6.

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Ghillani-Dalbin, P., C. Daubrosse, V. Mercier, et al. "AB1514 PERFORMANCE OF A NOVEL, FULLY AUTOMATED MULTIPLEXED IMMUNOASSAY MICROARRAY FOR THE SEROLOGICAL DETECTION OF ELEVEN AUTOANTIBODIES COMMONLY FOUND IN CONNECTIVE TISSUE DISEASES AND CLINICAL DIAGNOSIS OF REACTIVE SAMPLES." In EULAR 2024 European Congress of Rheumatology, 12-15 June. Vienna, Austria. BMJ Publishing Group Ltd and European League Against Rheumatism, 2024. http://dx.doi.org/10.1136/annrheumdis-2024-eular.4826.

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Klüpfel, J., M. Elsner, M. Seidel, U. Protzer, O. Hayden, and M. Ungerer. "3.6 - Detektion von Antikörpern gegen SARS-CoV-2 und ihrem Neutralisationspotential mittels Microarray-Immunoassays." In 15. Dresdner Sensor-Symposium 2021. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2021. http://dx.doi.org/10.5162/15dss2021/3.6.

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Luche, DD. "SARS-COV-2 NT-CHIP, A NOVEL FUNCTIONAL MULTIPLEX IMMUNOASSAY FOR SARS-COV-2 EVALUATION OF HUMORAL RESPONSE AND DETECTION OF NEUTRALIZING ANTIBODIES." In Resumos do 54º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2022. http://dx.doi.org/10.5327/1516-3180.140s1.7075.

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Objective: Here we present the initial development and characterization of the Sars-CoV-2 NT-Chip. This test allows combining general detection of binding antibodies against RBDs (humoral signature) and, in the same assay, to detect and quantify the subset of these antibodies that promote functional neutralization against Sars-CoV-2 infection. Moreover, high detection of antibodies against N suggests previous infection for Omicron-positive samples. Method: The Sars-CoV-2 VOC Virachip IgG test was first developed to promote a multiplex detection of binding antibodies against the Sars-CoV-2 prot
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