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1

Ogram, Andrew, Ashvini Chauhan, Kanika Sharma Inglett, Krish Jayachandran, and Susan Newman. "Microbial Ecology and Everglades Restoration." Critical Reviews in Environmental Science and Technology 41, sup1 (February 17, 2011): 289–308. http://dx.doi.org/10.1080/10643389.2010.531205.

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2

Harris, Jim. "Soil Microbial Communities and Restoration Ecology: Facilitators or Followers?" Science 325, no. 5940 (July 30, 2009): 573–74. http://dx.doi.org/10.1126/science.1172975.

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Microorganisms have critical roles in the functioning of soil in nutrient cycling, structural formation, and plant interactions, both positive and negative. These roles are important in reestablishing function and biodiversity in ecosystem restoration. Measurement of the community indicates the status of the system in relation to restoration targets and the effectiveness of management interventions, and manipulation of the community shows promise in the enhancement of the rate of recovery of degraded systems.
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3

Peralta, Ariane L., Jeffrey W. Matthews, and Angela D. Kent. "Microbial Community Structure and Denitrification in a Wetland Mitigation Bank." Applied and Environmental Microbiology 76, no. 13 (May 7, 2010): 4207–15. http://dx.doi.org/10.1128/aem.02977-09.

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ABSTRACT Wetland mitigation is implemented to replace ecosystem functions provided by wetlands; however, restoration efforts frequently fail to establish equivalent levels of ecosystem services. Delivery of microbially mediated ecosystem functions, such as denitrification, is influenced by both the structure and activity of the microbial community. The objective of this study was to compare the relationship between soil and vegetation factors and microbial community structure and function in restored and reference wetlands within a mitigation bank. Microbial community composition was assessed using terminal restriction fragment length polymorphism targeting the 16S rRNA gene (total bacteria) and the nosZ gene (denitrifiers). Comparisons of microbial function were based on potential denitrification rates. Bacterial community structures differed significantly between restored and reference wetlands; denitrifier community assemblages were similar among reference sites but highly variable among restored sites throughout the mitigation bank. Potential denitrification was highest in the reference wetland sites. These data demonstrate that wetland restoration efforts in this mitigation bank have not successfully restored denitrification and that differences in potential denitrification rates may be due to distinct microbial assemblages observed in restored and reference (natural) wetlands. Further, we have identified gradients in soil moisture and soil fertility that were associated with differences in microbial community structure. Microbial function was influenced by bacterial community composition and soil fertility. Identifying soil factors that are primary ecological drivers of soil bacterial communities, especially denitrifying populations, can potentially aid the development of predictive models for restoration of biogeochemical transformations and enhance the success of wetland restoration efforts.
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4

Turley, Nash E., Lukas Bell‐Dereske, Sarah E. Evans, and Lars A. Brudvig. "Agricultural land‐use history and restoration impact soil microbial biodiversity." Journal of Applied Ecology 57, no. 5 (March 6, 2020): 852–63. http://dx.doi.org/10.1111/1365-2664.13591.

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5

Lynum, Christopher A., Ashley N. Bulseco, Courtney M. Dunphy, Sean M. Osborne, Joseph H. Vineis, and Jennifer L. Bowen. "Microbial Community Response to a Passive Salt Marsh Restoration." Estuaries and Coasts 43, no. 6 (March 10, 2020): 1439–55. http://dx.doi.org/10.1007/s12237-020-00719-y.

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6

Banning, Natasha C., Deirdre B. Gleeson, Andrew H. Grigg, Carl D. Grant, Gary L. Andersen, Eoin L. Brodie, and D. V. Murphy. "Soil Microbial Community Successional Patterns during Forest Ecosystem Restoration." Applied and Environmental Microbiology 77, no. 17 (July 1, 2011): 6158–64. http://dx.doi.org/10.1128/aem.00764-11.

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ABSTRACTSoil microbial community characterization is increasingly being used to determine the responses of soils to stress and disturbances and to assess ecosystem sustainability. However, there is little experimental evidence to indicate that predictable patterns in microbial community structure or composition occur during secondary succession or ecosystem restoration. This study utilized a chronosequence of developing jarrah (Eucalyptus marginata) forest ecosystems, rehabilitated after bauxite mining (up to 18 years old), to examine changes in soil bacterial and fungal community structures (by automated ribosomal intergenic spacer analysis [ARISA]) and changes in specific soil bacterial phyla by 16S rRNA gene microarray analysis. This study demonstrated that mining in these ecosystems significantly altered soil bacterial and fungal community structures. The hypothesis that the soil microbial community structures would become more similar to those of the surrounding nonmined forest with rehabilitation age was broadly supported by shifts in the bacterial but not the fungal community. Microarray analysis enabled the identification of clear successional trends in the bacterial community at the phylum level and supported the finding of an increase in similarity to nonmined forest soil with rehabilitation age. Changes in soil microbial community structure were significantly related to the size of the microbial biomass as well as numerous edaphic variables (including pH and C, N, and P nutrient concentrations). These findings suggest that soil bacterial community dynamics follow a pattern in developing ecosystems that may be predictable and can be conceptualized as providing an integrated assessment of numerous edaphic variables.
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7

Robinson, Courtney J., Brendan J. M. Bohannan, and Vincent B. Young. "From Structure to Function: the Ecology of Host-Associated Microbial Communities." Microbiology and Molecular Biology Reviews 74, no. 3 (September 2010): 453–76. http://dx.doi.org/10.1128/mmbr.00014-10.

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SUMMARY In the past several years, we have witnessed an increased interest in understanding the structure and function of the indigenous microbiota that inhabits the human body. It is hoped that this will yield novel insight into the role of these complex microbial communities in human health and disease. What is less appreciated is that this recent activity owes a great deal to the pioneering efforts of microbial ecologists who have been studying communities in non-host-associated environments. Interactions between environmental microbiologists and human microbiota researchers have already contributed to advances in our understanding of the human microbiome. We review the work that has led to these recent advances and illustrate some of the possible future directions for continued collaboration between these groups of researchers. We discuss how the application of ecological theory to the human-associated microbiota can lead us past descriptions of community structure and toward an understanding of the functions of the human microbiota. Such an approach may lead to a shift in the prevention and treatment of human diseases that involves conservation or restoration of the normal community structure and function of the host-associated microbiota.
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8

Bobuľská, Lenka, Lenka Demková, Andrea Čerevková, and Marek Renčo. "Impact of Peatland Restoration on Soil Microbial Activity and Nematode Communities." Wetlands 40, no. 4 (November 12, 2019): 865–75. http://dx.doi.org/10.1007/s13157-019-01214-2.

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9

Hamman, Sarah T., and Christine V. Hawkes. "Biogeochemical and Microbial Legacies of Non‐Native Grasses Can Affect Restoration Success." Restoration Ecology 21, no. 1 (April 30, 2012): 58–66. http://dx.doi.org/10.1111/j.1526-100x.2011.00856.x.

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10

Plassart, Pierre, Marthe Akpa Vinceslas, Christophe Gangneux, Anne Mercier, Sylvie Barray, and Karine Laval. "Molecular and functional responses of soil microbial communities under grassland restoration." Agriculture, Ecosystems & Environment 127, no. 3-4 (September 2008): 286–93. http://dx.doi.org/10.1016/j.agee.2008.04.008.

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11

Moreno, Beatriz, Rogelio Nogales, Cristina Macci, Grazia Masciandaro, and Emilio Benitez. "Microbial eco-physiological profiles to estimate the biological restoration of a trichloroethylene-contaminated soil." Ecological Indicators 11, no. 6 (November 2011): 1563–71. http://dx.doi.org/10.1016/j.ecolind.2011.03.026.

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12

Wang, Jichen, Jiang Wang, Ji‐Zheng He, Yong‐Guan Zhu, Neng‐Hu Qiao, and Yuan Ge. "Arbuscular mycorrhizal fungi and plant diversity drive restoration of nitrogen‐cycling microbial communities." Molecular Ecology 30, no. 16 (July 2, 2021): 4133–46. http://dx.doi.org/10.1111/mec.16030.

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13

Smith, R. S., R. S. Shiel, R. D. Bardgett, D. Millward, P. Corkhill, P. Evans, H. Quirk, P. J. Hobbs, and S. T. Kometa. "Long-term change in vegetation and soil microbial communities during the phased restoration of traditional meadow grassland." Journal of Applied Ecology 45, no. 2 (April 2008): 670–79. http://dx.doi.org/10.1111/j.1365-2664.2007.01425.x.

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14

Smith, R. S., R. S. Shiel, R. D. Bardgett, D. Millward, P. Corkhill, G. Rolph, P. J. Hobbs, and S. Peacock. "Soil microbial community, fertility, vegetation and diversity as targets in the restoration management of a meadow grassland." Journal of Applied Ecology 40, no. 1 (February 2003): 51–64. http://dx.doi.org/10.1046/j.1365-2664.2003.00780.x.

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15

Birnbaum, Christina, and Eleonora Egidi. "Editorial: Special thematic issue on applying microbial community research to improve conservation and restoration outcomes." Plant Ecology 221, no. 9 (August 11, 2020): 749–51. http://dx.doi.org/10.1007/s11258-020-01068-3.

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16

Xiao, Haibing, Zhongwu Li, Chuxiong Deng, Lin Liu, Jia Chen, Bin Huang, Xiaodong Nie, Chun Liu, Danyang Wang, and Jieyu Jiang. "Autotrophic Bacterial Community and Microbial CO2 Fixation Respond to Vegetation Restoration of Eroded Agricultural Land." Ecosystems 22, no. 8 (April 1, 2019): 1754–66. http://dx.doi.org/10.1007/s10021-019-00369-7.

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17

Xue, Sha, Guobin Liu, Quanhou Dai, Xue Lan, and Na Yu. "Effects of different vegetation restoration models on soil microbial biomass in eroded hilly Loess Plateau, China." Frontiers of Forestry in China 2, no. 4 (October 2007): 376–81. http://dx.doi.org/10.1007/s11461-007-0060-x.

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18

Xue, Sha, Guobin Liu, Quanhou Dai, Chao Zhang, and Na Yu. "Evolution of soil microbial biomass in restoration process of Robinia pseudoacacia plantations in an eroded environment." Frontiers of Forestry in China 3, no. 3 (July 1, 2008): 293–99. http://dx.doi.org/10.1007/s11461-008-0040-9.

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19

Kao-Kniffin, J., and T. C. Balser. "Soil Microbial Composition and Nitrogen Cycling in a Disturbed Wet Prairie Restoration (Wisconsin)." Ecological Restoration 28, no. 1 (February 16, 2010): 20–22. http://dx.doi.org/10.3368/er.28.1.20.

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20

Bernhard, Anne E., David Marshall, and Lazaros Yiannos. "Increased Variability of Microbial Communities in Restored Salt Marshes nearly 30 Years After Tidal Flow Restoration." Estuaries and Coasts 35, no. 4 (April 4, 2012): 1049–59. http://dx.doi.org/10.1007/s12237-012-9502-2.

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21

Waterhouse, Benjamin R., Karen L. Adair, Stéphane Boyer, and Steve D. Wratten. "Advanced mine restoration protocols facilitate early recovery of soil microbial biomass, activity and functional diversity." Basic and Applied Ecology 15, no. 7 (November 2014): 599–606. http://dx.doi.org/10.1016/j.baae.2014.09.001.

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22

Tranvik, Lars J., Wilhelm Granéli, and Gunnar Gahnström. "Microbial Activity in Acidified and Limed Humic Lakes." Canadian Journal of Fisheries and Aquatic Sciences 51, no. 11 (November 1, 1994): 2529–36. http://dx.doi.org/10.1139/f94-252.

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To test the hypothesis that acidification negatively affects microbial processes in water and sediment, five acidified (pH of surface water 5.2 ± 0.3) and five limed (pH 6.9 ± 0.2) humic (water colour 91 ± 15 and 77 ± 28 mg Pt/L, respectively) lakes in southern Sweden were studied. Sediment pH of the acidified lakes was significantly lower than in limed lakes down to a sediment depth of at least 3 cm. In spite of this, there was no difference between acid and limed lakes with respect to bacterial abundance in the sediment, sediment oxygen uptake, or utilization of amino acids by sediment bacteria. Similarly, there was no difference in biological water column parameters, i.e., chlorophyll a, gross and net oxygen production, respiration, bacterial abundance, and the utilization of amino acids by pelagic bacteria. Assuming that the restoration of pH by liming also restores pre-acidification conditions of microbial life, this study suggests that the level of acidification typical for most areas does not influence microbial activity negatively.
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23

Schimann, Heidy, Richard Joffre, Jean-Christophe Roggy, Robert Lensi, and Anne-Marie Domenach. "Evaluation of the recovery of microbial functions during soil restoration using near-infrared spectroscopy." Applied Soil Ecology 37, no. 3 (November 2007): 223–32. http://dx.doi.org/10.1016/j.apsoil.2007.07.001.

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24

Tejada, Manuel, Concepción Benítez, Isidoro Gómez, and Juan Parrado. "Use of biostimulants on soil restoration: Effects on soil biochemical properties and microbial community." Applied Soil Ecology 49 (September 2011): 11–17. http://dx.doi.org/10.1016/j.apsoil.2011.07.009.

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25

Xiao, Lie, Guo-bin Liu, Jiao-yang Zhang, and Sha Xue. "Long-term effects of vegetational restoration on soil microbial communities on the Loess Plateau of China." Restoration Ecology 24, no. 6 (May 16, 2016): 794–804. http://dx.doi.org/10.1111/rec.12374.

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26

Andersen, Alan N., and Graham P. Sparling. "Ants as Indicators of Restoration Success: Relationship with Soil Microbial Biomass in the Australian Seasonal Tropics." Restoration Ecology 5, no. 2 (June 1997): 109–14. http://dx.doi.org/10.1046/j.1526-100x.1997.09713.x.

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27

Balshor, Bailey J., Matthew S. Garrambone, Paige Austin, Kathleen R. Balazs, Claudia Weihe, Jennifer B. H. Martiny, Travis E. Huxman, Johannah R. McCollum, and Sarah Kimball. "The effect of soil inoculants on seed germination of native and invasive species." Botany 95, no. 5 (May 2017): 469–80. http://dx.doi.org/10.1139/cjb-2016-0248.

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Successful reintroduction of native species through ecological restoration requires understanding the complex process of seed germination. Soil microbes play an important role in promoting native establishment and are often added to restoration sites during seed sowing. We tested the role of soil- and lab-grown bacterial inoculants on germination timing and percent germination for 19 species of plants commonly found in coastal California. Each species exhibited a different response to the inoculant treatments, but overall time-to-germination was longer and percent germination was lower with the soil inoculant compared with the control or other treatments. The invasive species in our study had the highest percent germination of all species and germinated faster than all native shrubs. Germination timing was negatively correlated with percent germination and with seed mass. Our results suggest that lab-grown inoculant and chemical treatment are effective at increasing germination in some native species, whereas soil inoculant is not. Given differences in germination timing between native and invasive species, restoration practitioners could consider using herbicide to treat areas seeded with native shrubs immediately following germination of invasive species without harming most natives, although germination timing and herbicides need further study in relation to microbial effects on seed germination.
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Smith, Jason M., Hector Castro, and Andrew Ogram. "Structure and Function of Methanogens along a Short-Term Restoration Chronosequence in the Florida Everglades." Applied and Environmental Microbiology 73, no. 13 (April 20, 2007): 4135–41. http://dx.doi.org/10.1128/aem.02557-06.

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ABSTRACT The removal of plants and soil to bedrock to eradicate exotic invasive plants within the Hole-in-the-Donut (HID) region, part of the Everglades National Park (Florida), presented a unique opportunity to study the redevelopment of soil and the associated microbial communities in the context of short-term primary succession and ecosystem restoration. The goal of this study was to identify relationships between soil redevelopment and activity and composition of methanogenic assemblages in HID soils. Methane production potentials indicated a general decline in methanogenic activity with restoration age. Microcosm incubations strongly suggested hydrogenotrophic methanogenesis as the most favorable pathway for methane formation in HID soils from all sites. Culture-independent techniques targeting methyl coenzyme M reductase genes (mcrA) were used to assess the dynamics of methanogenic assemblages. Clone libraries were dominated by sequences related to hydrogenotrophic methanogens of the orders Methanobacteriales and Methanococcales and suggested a general decline in the relative abundance of Methanobacteriales mcrA with time since restoration. Terminal restriction fragment length polymorphism analysis indicated methanogenic assemblages remain relatively stable between wet and dry seasons. Interestingly, analysis of soils across the restoration chronosequence indicated a shift in Methanobacteriales populations with restoration age, suggesting genotypic shifts due to site-specific factors.
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29

Watts, Corinne H., Maja Vojvodic-Vukovic, Greg C. Arnold, and Raphael K. Didham. "A comparison of restoration techniques to accelerate recovery of litter decomposition and microbial activity in an experimental peat bog restoration trial." Wetlands Ecology and Management 16, no. 3 (November 24, 2007): 199–217. http://dx.doi.org/10.1007/s11273-007-9068-0.

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30

Du, Wanlin, Yang Liu, Jinhui Sun, Naicheng Wu, Yongzhan Mai, and Chao Wang. "The aquatic microbial community: a bibliometric analysis of global research trends (1991– 2018)." Fundamental and Applied Limnology / Archiv für Hydrobiologie 194, no. 1 (August 31, 2020): 19–32. http://dx.doi.org/10.1127/fal/2020/1305.

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We performed a bibliometric analysis of relevant research trends, based on academic articles about the aquatic microbial community and recorded in the Web of Science during 1991–2018. The number of publications per annum is clearly rising and began to grow rapidly in 2005. Developed countries (e.g.the USA and some European countries) published the most articles, and led international cooperation. International cooperation benefitted from the implementation of the European Union (EU) Water Framework Directive and from the origination and development of molecular biological techniques. A strong correlation existed among such key words as "bacteria", "DGGE" (Denaturing Gradient Gel Electrophoresis), "16S rRNA", "pyrosequencing" and "sediment" as key research directions for many years. Sediment, biofilm and wetland were the main habitats studied; and high-throughput sequencing gradually replaced the traditional DGGE and other technologies, remaining the most popular research method at present. Studies still focus on basic research; interest in microbial community composition, structure, diversity and ecology remains high; and metagenomics and the microbiome have received considerable attention recently. Key words such as "organic matter", "nutrient", "enzyme activity", "nitrification", "denitrification" and "cyanobacteria" indicate current research hotspots, and we suggest this is because increasing attention is paid to environmental protection and management of the water environment by aquatic microorganisms. We predict that future research will promote the ultimate goals of warning about threats to the water environment and restoration by investigating the function of the aquatic microbial community.
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31

Ma, Jing, Yongqiang Lu, Fu Chen, Xiaoxiao Li, Dong Xiao, and Hui Wang. "Molecular Ecological Network Complexity Drives Stand Resilience of Soil Bacteria to Mining Disturbances among Typical Damaged Ecosystems in China." Microorganisms 8, no. 3 (March 19, 2020): 433. http://dx.doi.org/10.3390/microorganisms8030433.

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Understanding the interactions of soil microbial species and how they responded to disturbances are essential to ecological restoration and resilience in the semihumid and semiarid damaged mining areas. Information on this, however, remains unobvious and deficiently comprehended. In this study, based on the high throughput sequence and molecular ecology network analysis, we have investigated the bacterial distribution in disturbed mining areas across three provinces in China, and constructed molecular ecological networks to reveal the interactions of soil bacterial communities in diverse locations. Bacterial community diversity and composition were classified measurably between semihumid and semiarid damaged mining sites. Additionally, we distinguished key microbial populations across these mining areas, which belonged to Proteobacteria, Acidobacteria, Actinobacteria, and Chloroflexi. Moreover, the network modules were significantly associated with some environmental factors (e.g., annual average temperature, electrical conductivity value, and available phosphorus value). The study showed that network interactions were completely different across the different mining areas. The keystone species in different mining areas suggested that selected microbial communities, through natural successional processes, were able to resist the corresponding environment. Moreover, the results of trait-based module significances showed that several environmental factors were significantly correlated with some keystone species, such as OTU_8126 (Acidobacteria), OTU_8175 (Burkholderiales), and OTU_129 (Chloroflexi). Our study also implied that the complex network of microbial interaction might drive the stand resilience of soil bacteria in the semihumid and semiarid disturbed mining areas.
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32

Smith, Monique E., Steven Delean, Timothy R. Cavagnaro, and José M. Facelli. "Evidence for species-specific plant responses to soil microbial communities from remnant and degraded land provides promise for restoration." Austral Ecology 43, no. 3 (February 1, 2018): 301–8. http://dx.doi.org/10.1111/aec.12567.

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33

Strickland, Michael S., Mac A. Callaham, Emile S. Gardiner, John A. Stanturf, Jonathan W. Leff, Noah Fierer, and Mark A. Bradford. "Response of soil microbial community composition and function to a bottomland forest restoration intensity gradient." Applied Soil Ecology 119 (October 2017): 317–26. http://dx.doi.org/10.1016/j.apsoil.2017.07.008.

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34

Kim, Jong-Hwa, Kiyoung Kim, and Wonyong Kim. "Gut microbiota restoration through fecal microbiota transplantation: a new atopic dermatitis therapy." Experimental & Molecular Medicine 53, no. 5 (May 2021): 907–16. http://dx.doi.org/10.1038/s12276-021-00627-6.

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AbstractThe pathogenesis of atopic dermatitis (AD) involves complex factors, including gut microbiota and immune modulation, which remain poorly understood. The aim of this study was to restore gut microbiota via fecal microbiota transplantation (FMT) to ameliorate AD in mice. FMT was performed using stool from donor mice. The gut microbiota was characterized via 16S rRNA sequencing and analyzed using Quantitative Insights into Microbial Ecology 2 with the DADA2 plugin. Gut metabolite levels were determined by measuring fecal short-chain fatty acid (SCFA) contents. AD-induced allergic responses were evaluated by analyzing blood parameters (IgE levels and eosinophil percentage, eosinophil count, basophil percentage, and monocyte percentage), the levels of Th1 and Th2 cytokines, dermatitis score, and the number of mast cells in the ileum and skin tissues. Calprotectin level was measured to assess gut inflammation after FMT. FMT resulted in the restoration of gut microbiota to the donor state and increases in the levels of SCFAs as gut metabolites. In addition, FMT restored the Th1/Th2 balance, modulated Tregs through gut microbiota, and reduced IgE levels and the numbers of mast cells, eosinophils, and basophils. FMT is associated with restoration of gut microbiota and immunologic balance (Th1/Th2) along with suppression of AD-induced allergic responses and is thus a potential new therapy for AD.
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Requena, Natalia, Estefania Perez-Solis, Concepción Azcón-Aguilar, Peter Jeffries, and José-Miguel Barea. "Management of Indigenous Plant-Microbe Symbioses Aids Restoration of Desertified Ecosystems." Applied and Environmental Microbiology 67, no. 2 (February 1, 2001): 495–98. http://dx.doi.org/10.1128/aem.67.2.495-498.2001.

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ABSTRACT Disturbance of natural plant communities is the first visible indication of a desertification process, but damage to physical, chemical, and biological soil properties is known to occur simultaneously. Such soil degradation limits reestablishment of the natural plant cover. In particular, desertification causes disturbance of plant-microbe symbioses which are a critical ecological factor in helping further plant growth in degraded ecosystems. Here we demonstrate, in two long-term experiments in a desertified Mediterranean ecosystem, that inoculation with indigenous arbuscular mycorrhizal fungi and with rhizobial nitrogen-fixing bacteria not only enhanced the establishment of key plant species but also increased soil fertility and quality. The dual symbiosis increased the soil nitrogen (N) content, organic matter, and hydrostable soil aggregates and enhanced N transfer from N-fixing to nonfixing species associated within the natural succession. We conclude that the introduction of target indigenous species of plants associated with a managed community of microbial symbionts is a successful biotechnological tool to aid the recovery of desertified ecosystems.
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36

Jerman, V., M. Metje, I. Mandić-Mulec, and P. Frenzel. "Wetland restoration and methanogenesis: the activity of microbial populations and competition for substrates at different temperatures." Biogeosciences 6, no. 6 (June 29, 2009): 1127–38. http://dx.doi.org/10.5194/bg-6-1127-2009.

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Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period of 84 d at 15°C and a minimum of 7 d at 37°C, the optimum temperature for methanogenesis. This lag was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.
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Jerman, V., M. Metje, I. Mandić-Mulec, and P. Frenzel. "Wetland restoration and methanogenesis: the activity of microbial populations and competition for substrates at different temperatures." Biogeosciences Discussions 6, no. 1 (February 24, 2009): 2357–86. http://dx.doi.org/10.5194/bgd-6-2357-2009.

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Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period and was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.
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Haldar, Shyamalina, and Sanghamitra Sengupta. "Plant-microbe Cross-talk in the Rhizosphere: Insight and Biotechnological Potential." Open Microbiology Journal 9, no. 1 (March 31, 2015): 1–7. http://dx.doi.org/10.2174/1874285801509010001.

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Rhizosphere, the interface between soil and plant roots, is a chemically complex environment which supports the development and growth of diverse microbial communities. The composition of the rhizosphere microbiome is dynamic and controlled by multiple biotic and abiotic factors that include environmental parameters, physiochemical properties of the soil, biological activities of the plants and chemical signals from the plants and bacteria which inhabit the soil adherent to root-system. Recent advancement in molecular and microbiological techniques has unravelled the interactions among rhizosphere residents at different levels. In this review, we elaborate on various factors that determine plant-microbe and microbe-microbe interactions in the rhizosphere, with an emphasis on the impact of host genotype and developmental stages which together play pivotal role in shaping the nature and diversity of root exudations. We also discuss about the coherent functional groups of microorganisms that colonize rhizosphere and enhance plant growth and development by several direct and indirect mechanisms. Insights into the underlying structural principles of indigenous microbial population and the key determinants governing rhizosphere ecology will provide directions for developing techniques for profitable applicability of beneficial microorganisms in sustainable agriculture and nature restoration.
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Tian, Guanglong, James M. Vose, David C. Coleman, Christopher D. Geron, and John T. Walker. "Evaluation of the effectiveness of riparian zone restoration in the southern Appalachians by assessing soil microbial populations." Applied Soil Ecology 26, no. 1 (May 2004): 63–68. http://dx.doi.org/10.1016/j.apsoil.2003.10.003.

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40

Chen, Jin, Li Mo, Zhechao Zhang, Ji Nan, Daolong Xu, Lumeng Chao, Xiaodong Zhang, and Yuying Bao. "Evaluation of the ecological restoration of a coal mine dump by exploring the characteristics of microbial communities." Applied Soil Ecology 147 (March 2020): 103430. http://dx.doi.org/10.1016/j.apsoil.2019.103430.

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41

Prasse, Christine E., Andrew H. Baldwin, and Stephanie A. Yarwood. "Site History and Edaphic Features Override the Influence of Plant Species on Microbial Communities in Restored Tidal Freshwater Wetlands." Applied and Environmental Microbiology 81, no. 10 (March 13, 2015): 3482–91. http://dx.doi.org/10.1128/aem.00038-15.

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ABSTRACTRestored wetland soils differ significantly in physical and chemical properties from their natural counterparts even when plant community compositions are similar, but effects of restoration on microbial community composition and function are not well understood. Here, we investigate plant-microbe relationships in restored and natural tidal freshwater wetlands from two subestuaries of the Chesapeake Bay. Soil samples were collected from the root zone ofTypha latifolia,Phragmites australis,Peltandra virginica, andLythrum salicaria. Soil microbial composition was assessed using 454 pyrosequencing, and genes representing bacteria, archaea, denitrification, methanogenesis, and methane oxidation were quantified. Our analysis revealed variation in some functional gene copy numbers between plant species within sites, but intersite comparisons did not reveal consistent plant-microbe trends. We observed more microbial variations between plant species in natural wetlands, where plants have been established for a long period of time. In the largest natural wetland site, sequences putatively matching methanogens accounted for ∼17% of all sequences, and the same wetland had the highest numbers of genes coding for methane coenzyme A reductase (mcrA). Sequences putatively matching aerobic methanotrophic bacteria and anaerobic methane-oxidizing archaea (ANME) were detected in all sites, suggesting that both aerobic and anaerobic methane oxidation are possible in these systems. Our data suggest that site history and edaphic features override the influence of plant species on microbial communities in restored wetlands.
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42

Ramirez, Kelly. "Microbes matter: integrating microbial sequence information for biodiversity." Biodiversity Information Science and Standards 2 (May 18, 2018): e26009. http://dx.doi.org/10.3897/biss.2.26009.

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The large efforts to document and map aboveground biodiversity have helped to elucidate ecological and evolutionary mechanisms and processes, predict responses to global change, and identify potential management options in response to those changes. Yet these concepts have mostly been applied to aboveground plant and animal communities, while microbial diversity remains difficult to incorporate. The ability to integrate microbial sequence data into an accessible global infrastructure has previously been limited by a few key factors: First, most of microbial diversity remains undescribed and unknown; there is just an enormous amount of biodiversity. Second, there is a lack of congruence between the many disparate microbial datasets (e.g. taxonomy, phylogeny, and methodological biases), which limits the ability to monitor and quantify global patterns of the terrestrial microbiome. Finally, there is a lack of coordination and networking between scientists studying microbes. In this presentation I will discuss two case studies that highlight how we can begin to link microbial data to the already well-established macro-knowledge and other environmental databases (like global carbon maps) Study 1 – a megameta analysis: The emergence of high-throughput DNA sequencing methods provides unprecedented opportunities to further unravel microbial ecology and its worldwide role from human health to ecosystem functioning. However, in spite of the abundance of sequencing studies, combining data from multiple individual studies to address macroecological questions of bacterial diversity remains methodically challenging and plagued with biases. While previous meta-analysis efforts have focused on diversity measures or abundances of major taxa, in a recent study(1) we show that disparate amplicon sequence data can be combined at the taxonomy-based level to assess bacterial community structure. Using a machine learning approach, we found that rarer taxa are more important for structuring soil communities than abundant taxa. We concluded that combining data from independent studies can be used to explore novel patterns in bacterial communities, identify potential ‘indicator’ taxa with an important role in structuring communities, and propose new hypotheses on the factors that shape microbial biogeography previously overlooked. Study 2 – a global soil biodiversity database: Greater access to microbial data is an important next step for biodiversity research and conservation, and for understanding the ecology and evolution of microbial communities. In collaboration with the Global Soil Biodiversity Initiative and the German Biodiversity Synthesis Centre (sDIV) we outlined steps that must be taken to ensure microbial sequence data can be included in global measures and maps of biodiversity(2). Here I will discuss how the plant associated microbiome is an optimal starting point to synthesize microbial sequence data on an open and global platform. The plant-microbiome is an optimal model system that goes across scales and time, can act as a bridge between microorganisms and macroorganisms, and as an opportunity to more thoroughly explore the synthesis of global microbial sequence data (for a global soil biodiversity database). Beyond expanding primary research, the patterns discovered in a synthesis of plant-microbiome can be used to explore and guide ecosystem restoration and sustainability. Overall, a better understanding of microbial biodiversity will help to predict consequences of (human-induced) global changes and facilitate conservation and adaptation responses. (1) Ramirez, K.S., C.G. Knight et al. and F.T. de Vries (2017). Detecting macroecological patterns in bacterial communities across independent studies of global soils. Nature Microbiology. (2) Ramirez, K.S., M. Döring, N. Eisenhauer, C. Gardi, J. Ladau, J.W. Leff, G. Lentendu, Z. Lindo, M.C. Rillig, D. Russell, S. Scheu, M.G. St. John, F.T. de Vries, T. Wubet, W.H. van der Putten, D.H. Wall, (2015). Towards a global platform for linking soil biodiversity data. Frontiers in Ecology and Evolutionary Biology 3(91). doi: 10.3389/fevo.2015.00091
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Miller, Nicole, Paul Maneval, Carrie Manfrino, Thomas K. Frazer, and Julie L. Meyer. "Spatial distribution of microbial communities among colonies and genotypes in nursery-reared Acropora cervicornis." PeerJ 8 (August 26, 2020): e9635. http://dx.doi.org/10.7717/peerj.9635.

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Background The architecturally important coral species Acropora cervicornis and A. palmata were historically common in the Caribbean, but have declined precipitously since the early 1980s. Substantial resources are currently being dedicated to coral gardening and the subsequent outplanting of asexually reproduced colonies of Acropora, activities that provide abundant biomass for both restoration efforts and for experimental studies to better understand the ecology of these critically endangered coral species. Methods We characterized the bacterial and archaeal community composition of A. cervicornis corals in a Caribbean nursery to determine the heterogeneity of the microbiome within and among colonies. Samples were taken from three distinct locations (basal branch, intermediate branch, and branch tip) from colonies of three different coral genotypes. Results Overall, microbial community composition was similar among colonies due to high relative abundances of the Rickettsiales genus MD3-55 (Candidatus Aquarickettsia) in nearly all samples. While microbial communities were not different among locations within the same colony, they were significantly different between coral genotypes. These findings suggest that sampling from any one location on a coral host is likely to provide a representative sample of the microbial community for the entire colony. Our results also suggest that subtle differences in microbiome composition may be influenced by the coral host, where different coral genotypes host slightly different microbiomes. Finally, this study provides baseline data for future studies seeking to understand the microbiome of nursery-reared A. cervicornis and its roles in coral health, adaptability, and resilience.
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44

ANTONSEN, HILDE, and PÅL AXEL OLSSON. "Relative importance of burning, mowing and species translocation in the restoration of a former boreal hayfield: responses of plant diversity and the microbial community." Journal of Applied Ecology 42, no. 2 (April 18, 2005): 337–47. http://dx.doi.org/10.1111/j.1365-2664.2005.01023.x.

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45

De Deyn, G. B., H. Quirk, S. Oakley, N. Ostle, and R. D. Bardgett. "Rapid transfer of photosynthetic carbon through the plant-soil system in differently managed grasslands." Biogeosciences Discussions 8, no. 1 (February 2, 2011): 921–40. http://dx.doi.org/10.5194/bgd-8-921-2011.

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Abstract. Plant-soil interactions are central to short-term carbon (C) cycling through the rapid transfer of recently assimilated C from plant roots to soil biota. In grassland ecosystems, changes in C cycling are likely to be influenced by land use and management that changes vegetation and the associated soil microbial communities. Here we tested whether changes in grassland vegetation composition resulting from management for plant diversity influences short-term rates of C assimilation, retention and transfer from plants to soil microbes. To do this, we used an in situ 13C-CO2 pulse-labeling approach to measure differential C uptake among different plant species and the transfer of the plant-derived 13C to key groups of soil microbiota across selected treatments of a long-term plant diversity grassland restoration experiment. Results showed that plant taxa differed markedly in the rate of 13C assimilation and retention: uptake was greatest and retention lowest in Ranunculus repens, and assimilation was least and retained longest in mosses. Incorporation of recent plant-derived 13C was maximal in all microbial phosopholipid fatty acid (PLFA) markers at 24 h after labeling. The greatest incorporation of 13C was in the PLFA 16:1ω5, a marker for arbuscular mycorrhizal fungi (AMF), while after one week most 13C was retained in the PLFA 18:2ω6,9 which is indicative of assimilation of plant-derived 13C by saprophytic fungi. Our results of 13C assimilation, transfer and retention within plant species and soil microbes were consistent across management treatments. Overall, our findings suggest that changes in vegetation and soil microbial composition resulting from differences in long-term grassland management will affect short-term cycling of photosynthetic C, but that restoration management does not alter the short-term C uptake and transfer within plant species and within key groups of soil microbes. Moreover, across all treatments we found that plant-derived C is rapidly transferred specifically to AMF and decomposer fungi, indicating their consistent key role in the cycling of recent plant derived C.
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46

Schmidt, Katharina T., Mia Maltz, Priscilla Ta, Banafshe Khalili, Claudia Weihe, Michala Phillips, Emma Aronson, Megan Lulow, Jennifer Long, and Sarah Kimball. "Identifying Mechanisms for Successful Ecological Restoration with Salvaged Topsoil in Coastal Sage Scrub Communities." Diversity 12, no. 4 (April 14, 2020): 150. http://dx.doi.org/10.3390/d12040150.

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Although aboveground metrics remain the standard, restoring functional ecosystems should promote both aboveground and belowground biotic communities. Restoration using salvaged soil—removal and translocation of topsoil from areas planned for development, with subsequent deposition at degraded sites—is an alternative to traditional methods. Salvaged soil contains both seed and spore banks, which may holistically augment restoration. Salvaged soil methods may reduce non-native germination by burying non-native seeds, increase native diversity by adding native seeds, or transfer soil microbiomes, including arbuscular mycorrhizal fungi (AMF), to recipient sites. We transferred soil to three degraded recipient sites and monitored soil microbes, using flow cytometry and molecular analyses, and characterized the plant community composition. Our findings suggest that salvaged soil at depths ≥5 cm reduced non-native grass cover and increased native plant density and species richness. Bacterial abundance at recipient sites were statistically equivalent to donor sites in abundance. Overall, topsoil additions affected AMF alpha diversity and community composition and increased rhizophilic AMF richness. Because salvaged soil restoration combines multiple soil components, including native plant and microbial propagules, it may promote both aboveground and belowground qualities of the donor site, when applying this method for restoring invaded and degraded ecosystems.
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Fu, Denggao, Xiaoni Wu, Qingtian Qiu, Changqun Duan, and Davey L. Jones. "Seasonal variations in soil microbial communities under different land restoration types in a subtropical mountains region, Southwest China." Applied Soil Ecology 153 (September 2020): 103634. http://dx.doi.org/10.1016/j.apsoil.2020.103634.

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48

Williams, Wendy, Angela Chilton, Mel Schneemilch, Stephen Williams, Brett Neilan, and Colin Driscoll. "Microbial biobanking – cyanobacteria-rich topsoil facilitates mine rehabilitation." Biogeosciences 16, no. 10 (May 28, 2019): 2189–204. http://dx.doi.org/10.5194/bg-16-2189-2019.

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Abstract. Restoration of soils post-mining requires key solutions to complex issues through which the disturbance of topsoil incorporating soil microbial communities can result in a modification to ecosystem function. This research was in collaboration with Iluka Resources at the Jacinth–Ambrosia (J–A) mineral sand mine located in a semi-arid chenopod shrubland in southern Australia. At J–A, assemblages of microorganisms and microflora inhabit at least half of the soil surfaces and are collectively known as biocrusts. This research encompassed a polyphasic approach to soil microbial community profiling focused on “biobanking” viable cyanobacteria in topsoil stockpiles to facilitate rehabilitation. We found that cyanobacterial communities were compositionally diverse topsoil microbiomes. There was no significant difference in cyanobacterial community structure across soil types. As hypothesised, cyanobacteria were central to soil microprocesses, strongly supported by species richness and diversity. Cyanobacteria were a significant component of all three successional stages with 21 species identified from 10 sites. Known nitrogen-fixing cyanobacteria Symploca, Scytonema, Porphyrosiphon, Brasilonema, Nostoc, and Gloeocapsa comprised more than 50 % of the species richness at each site and 61 % of the total community richness. In the first study of its kind, we have described the response of cyanobacteria to topsoil stockpiling at various depths and ages. Cyanobacteria are moderately resilient to stockpiling at depth and over time, with average species richness greatest in the top 10 cm of the stockpiles of all ages and more viable within the first 6 weeks, indicating potential for biocrust re-establishment. In general, the resilience of cyanobacteria to burial in topsoil stockpiles in both the short and long term was significant; however, in an arid environment recolonisation and community diversity could be impeded by drought. Biocrust re-establishment during mine rehabilitation relies on the role of cyanobacteria as a means of early soil stabilisation. At J–A mine operations do not threaten the survival of any of the organisms we studied. Increased cyanobacterial biomass is likely to be a good indicator and reliable metric for the re-establishment of soil microprocesses.
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Mendez, Monica O., Julia W. Neilson, and Raina M. Maier. "Characterization of a Bacterial Community in an Abandoned Semiarid Lead-Zinc Mine Tailing Site." Applied and Environmental Microbiology 74, no. 12 (April 18, 2008): 3899–907. http://dx.doi.org/10.1128/aem.02883-07.

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ABSTRACT Bacterial diversity in mine tailing microbial communities has not been thoroughly investigated despite the correlations that have been observed between the relative microbial diversity and the success of revegetation efforts at tailing sites. This study employed phylogenetic analyses of 16S rRNA genes to compare the bacterial communities present in highly disturbed, extremely (pH 2.7) and moderately (pH 5.7) acidic lead-zinc mine tailing samples from a semiarid environment with those from a vegetated off-site (OS) control sample (pH 8). Phylotype richness in these communities decreased from 42 in the OS control to 24 in the moderately acidic samples and 8 in the extremely acidic tailing samples. The clones in the extremely acidic tailing sample were most closely related to acidophiles, none of which were detected in the OS control sample. The comparison generated by this study between the bacteria present in extremely acidic tailing and that in moderately acidic tailing communities with those in an OS control soil provides a reference point from which to evaluate the successful restoration of mine tailing disposal sites by phytostabilization.
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Rahmeh, Rita, Abrar Akbar, Vinod Kumar, Hamad Al-Mansour, Mohamed Kishk, Nisar Ahmed, Mustafa Al-Shamali, et al. "Insights into Bacterial Community Involved in Bioremediation of Aged Oil-Contaminated Soil in Arid Environment." Evolutionary Bioinformatics 17 (January 2021): 117693432110168. http://dx.doi.org/10.1177/11769343211016887.

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Soil contamination by hydrocarbons due to oil spills has become a global concern and it has more implications in oil producing regions. Biostimulation is considered as one of the promising remediation techniques that can be adopted to enhance the rate of degradation of crude oil. The soil microbial consortia play a critical role in governing the biodegradation of total petroleum hydrocarbons (TPHs), in particular polycyclic aromatic hydrocarbons (PAHs). In this study, the degradation pattern of TPHs and PAHs of Kuwait soil biopiles was measured at three-month intervals. Then, the microbial consortium associated with oil degradation at each interval was revealed through 16S rRNA based next generation sequencing. Rapid degradation of TPHs and most of the PAHs was noticed at the first 3 months of biostimulation with a degradation rate of pyrene significantly higher compared to other PAHs counterparts. The taxonomic profiling of individual stages of remediation revealed that, biostimulation of the investigated soil favored the growth of Proteobacteria, Alphaprotobacteria, Chloroflexi, Chlorobi, and Acidobacteria groups. These findings provide a key step towards the restoration of oil-contaminated lands in the arid environment.
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