Journal articles: 'Microbially Induced Calcite Precipitation'

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Kim, Gunjo, and Heejung Youn. "Microbially Induced Calcite Precipitation Employing Environmental Isolates." Materials 9, no. 6 (June 15, 2016): 468. http://dx.doi.org/10.3390/ma9060468.

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Kim, Gunjo, Janghwan Kim, and Heejung Youn. "Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation." Applied Sciences 8, no. 8 (August 1, 2018): 1277. http://dx.doi.org/10.3390/app8081277.

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In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii.

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Kang, Chang-Ho, Sang-Hyun Han, YuJin Shin, Soo Ji Oh, and Jae-Seong So. "Bioremediation of Cd by Microbially Induced Calcite Precipitation." Applied Biochemistry and Biotechnology 172, no. 4 (December 3, 2013): 1929–37. http://dx.doi.org/10.1007/s12010-013-0626-z.

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Kang, Chang-Ho, Sang-Hyun Han, YuJin Shin, Soo Ji Oh, and Jae-Seong So. "Bioremediation of Cd by Microbially Induced Calcite Precipitation." Applied Biochemistry and Biotechnology 172, no. 6 (January 24, 2014): 2907–15. http://dx.doi.org/10.1007/s12010-014-0737-1.

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Al Qabany, Ahmed, Kenichi Soga, and Carlos Santamarina. "Factors Affecting Efficiency of Microbially Induced Calcite Precipitation." Journal of Geotechnical and Geoenvironmental Engineering 138, no. 8 (August 2012): 992–1001. http://dx.doi.org/10.1061/(asce)gt.1943-5606.0000666.

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Cunningham, A. B., H. Class, A. Ebigbo, R. Gerlach, A. J. Phillips, and J. Hommel. "Field-scale modeling of microbially induced calcite precipitation." Computational Geosciences 23, no. 2 (November 23, 2018): 399–414. http://dx.doi.org/10.1007/s10596-018-9797-6.

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Yuan, Xiao Lu, Shi Hua Zhou, Wei Min Hu, Sen Yao Tan, and Deng Pan. "Effect of Cement Type and Air-Entraining Agent on Microbially Induced Carbonate Precipitation in Cement Paste." Advanced Materials Research 816-817 (September 2013): 758–61. http://dx.doi.org/10.4028/www.scientific.net/amr.816-817.758.

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The effect of cement type and the air-entraining agent on microbially induced carbonate precipitation in cement paste has been studied. Results indicate that after biodeposition treatment, Sulphoaluminate cement paste behaved with a higher growth rate of compressive strength than OPC paste. Incorporation of air-entraining agent increased the growth rate of compressive strength of sulphoaluminate cement paste. Calcite was formed through microbially induced carbonate precipitation in cement pastes. Sulphoaluminate cement paste achieved a larger amount of calcite than OPC paste.

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Zhang, T., and I. Klapper. "Mathematical model of biofilm induced calcite precipitation." Water Science and Technology 61, no. 11 (June 1, 2010): 2957–64. http://dx.doi.org/10.2166/wst.2010.064.

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Microbially modulated carbonate precipitation is a fundamentally important phenomenon of both engineered and natural environments. In this paper, we propose a mixture model for biofilm induced calcite precipitation. The model consists of three phases – calcite, biofilm and solvent – which satisfy conservation of mass and momentum laws with addition of a free energy of mixing. The model also accounts for chemistry, mechanics, thermodynamics, fluid and electrodiffusion transport effects. Numerical simulations qualitatively capturing the dynamics of this process and revealing effects of kinetic parameters and external flow conditions are presented.

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Okyay, Tugba O., Hang N. Nguyen, Sarah L. Castro, and Debora F. Rodrigues. "CO2 sequestration by ureolytic microbial consortia through microbially-induced calcite precipitation." Science of The Total Environment 572 (December 2016): 671–80. http://dx.doi.org/10.1016/j.scitotenv.2016.06.199.

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Jeong, Jin-Hoon, Yoon-Soo Jo, Chang-Seon Park, Chang-Ho Kang, and Jae-Seong So. "Biocementation of Concrete Pavements Using Microbially Induced Calcite Precipitation." Journal of Microbiology and Biotechnology 27, no. 7 (July 28, 2017): 1331–35. http://dx.doi.org/10.4014/jmb.1701.01041.

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Venuleo, S., L. Laloui, D. Terzis, T. Hueckel, and M. Hassan. "Microbially induced calcite precipitation effect on soil thermal conductivity." Géotechnique Letters 6, no. 1 (March 2016): 39–44. http://dx.doi.org/10.1680/jgele.15.00125.

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Kang, Bo, Fusheng Zha, Weihao Deng, Runkai Wang, Xianguo Sun, and Zhitang Lu. "Biocementation of Pyrite Tailings Using Microbially Induced Calcite Carbonate Precipitation." Molecules 27, no. 11 (June 4, 2022): 3608. http://dx.doi.org/10.3390/molecules27113608.

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Tailing sand contains a large number of heavy metals and sulfides that are prone to forming acid mine drainage (AMD), which pollutes the surrounding surface environment and groundwater resources and damages the ecological environment. Microbially induced calcium carbonate precipitation (MICP) technology can biocement heavy metals and sulfides in tailing sand and prevent pollution via source control. In this study, through an unconfined compressive strength test, permeability test, and toxic leaching test (TCLP), the curing effect of MICP was investigated in the laboratory and the effect of grouting rounds on curing was also analyzed. In addition, the curing mechanism of MICP was studied by means of Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM). The experimental results showed that MICP could induce calcium carbonate precipitation through relatively complex biochemical and physicochemical reactions to achieve the immobilization of heavy metals and sulfides and significantly reduce the impact of tailing sand on the surrounding environment.

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Misiołek, Katarzyna, Paweł Popielski, and Katarzyna Affek. "Preliminary research for identification of bacteria useful in microbially induced calcium carbonate precipitation." E3S Web of Conferences 44 (2018): 00115. http://dx.doi.org/10.1051/e3sconf/20184400115.

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MICP (Microbially Induced Calcite Precipitation) is a new biological method in soil stabilization. This cheap and eco-friendly technique improves strength parameters of the ground such as shear strength and decreases the permeability of gravelly and sandy soil. There are variety of microorganisms that can be used in calcite precipitation. The most popular method is precipitation of calcium carbonate by bacteria. The main purpose of the article is to present the results from Gram staining of bacteria isolated from construction sites, which is the first step of their identification. Gram’s method allows to find out which morphological groups of bacteria are adapted to conditions present in soil from construction sites and therefore are potentially able to produce calcite. The article describes the methodology of isolation, staining and determination of morphological types of bacteria.

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Zúñiga-Barra, Héctor, Eduardo Ortega-Martínez, Javiera Toledo-Alarcón, Álvaro Torres-Aravena, Lorena Jorquera, Mariella Rivas, and David Jeison. "Potential Use of Microbially Induced Calcite Precipitation for the Biocementation of Mine Tailings." Minerals 13, no. 4 (April 1, 2023): 506. http://dx.doi.org/10.3390/min13040506.

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Mining activities offer clear economic benefits for mineral-rich countries. However, mining operations can produce several environmental impacts. Many of these are associated with generating and managing mining waste known as tailings, which are typically stored in surface facilities. Windblown dust emissions from tailing deposits can cause severe damage to local ecosystems and adverse health effects for the surrounding population. Microbially induced calcite precipitation (MICP) can be used for the superficial biocementation of tailings, thereby preventing such emissions. This research studied the capacity of MICP for the biocementation of tailings. The effect of applying different doses of biocementation reagents and two different methods for their application were evaluated. Results show that a relevant increase in surface strength can be achieved, especially if reagents are mechanically mixed with the tailings to induce a more homogeneous distribution of precipitates. Micrographical and mineralogical analysis by SEM, FTIR and XRD analysis showed the precipitation of calcium in the form of anorthite, calcite or vaterite. Overall results indicate that calcite precipitation can be induced in tailing by microorganisms with urease activity, providing a potential technique for the biocementation of this material.

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Yuan, Xiao Lu, Shi Hua Zhou, Wei Min Hu, and Dong Mei Liu. "Microbially Induced Carbonate Precipitation in Sulphoaluminate Cement Mortar." Applied Mechanics and Materials 454 (October 2013): 234–37. http://dx.doi.org/10.4028/www.scientific.net/amm.454.234.

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This paper investigated microbially induced carbonate precipitation in sulphoaluminate cement mortar. Urea and calcium of varied amounts were studied on the growth of microorganisms, the degradation of urea and the precipitation production. Compressive strength and flexural strength of sulphoaluminate cement mortar were measured and discussed. Results indicate that urea of 10g/L and calcium of 2mmol/L achieved favorable microorganism growth and the production of precipitation, which was composed of large amounts of calcite as well as small vaterite. Biodeposition increased the compressive strength and the flexural strength of sulphoaluminate cement mortar by 10% and 21%, respectively.

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Muthukkumaran, Kasinathan, and Bettadapura Subramanyam Shashank. "Durability of microbially induced calcite precipitation (micp) treated cohesionless soils." Japanese Geotechnical Society Special Publication 2, no. 56 (2016): 1946–49. http://dx.doi.org/10.3208/jgssp.ind-23.

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Wei, R., J. Z. Xiao, S. F. Wu, H. Cai, and Z. W. Wang. "Effectiveness of Microbially Induced Calcite Precipitation for Treating Expansive Soils." Advances in Civil Engineering Materials 10, no. 1 (August 19, 2021): 20200182. http://dx.doi.org/10.1520/acem20200182.

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Gao, Yufeng, Xinyi Tang, Jian Chu, and Jia He. "Microbially Induced Calcite Precipitation for Seepage Control in Sandy Soil." Geomicrobiology Journal 36, no. 4 (January 24, 2019): 366–75. http://dx.doi.org/10.1080/01490451.2018.1556750.

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Hu, Lei, Huiyao Wang, Pei Xu, and Yanyan Zhang. "Biomineralization of hypersaline produced water using microbially induced calcite precipitation." Water Research 190 (February 2021): 116753. http://dx.doi.org/10.1016/j.watres.2020.116753.

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Kang, Chang-Ho, Sang-Hyun Han, YuJin Shin, Soo Ji Oh, and Jae-Seong So. "Erratum to: Bioremediation of Cd by Microbially Induced Calcite Precipitation." Applied Biochemistry and Biotechnology 176, no. 2 (April 29, 2015): 645. http://dx.doi.org/10.1007/s12010-015-1558-6.

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Dikshit, Rashmi, Animesh Jain, Arjun Dey, and Aloke Kumar. "Microbially induced calcite precipitation using Bacillus velezensis with guar gum." PLOS ONE 15, no. 8 (August 12, 2020): e0236745. http://dx.doi.org/10.1371/journal.pone.0236745.

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22

Pacton, M., S. F. M. Breitenbach, F. A. Lechleitner, A. Vaks, C. Rollion-Bard, O. S. Gutareva, A. V. Osintcev, and C. Vasconcelos. "The role of microorganisms in the formation of a stalactite in Botovskaya Cave, Siberia – paleoenvironmental implications." Biogeosciences 10, no. 9 (September 27, 2013): 6115–30. http://dx.doi.org/10.5194/bg-10-6115-2013.

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Abstract. Calcitic speleothems in caves can form through abiogenic or biogenic processes, or through a combination of both. Many issues conspire to make the assessment of biogenicity difficult, especially when focusing on old speleothem deposits. This study reports on a multiproxy analysis of a Siberian stalactite, combining high-resolution microscopy, isotope geochemistry and microbially enhanced mineral precipitation laboratory experiments. The contact between growth layers in a stalactite exhibits a biogenic isotopic signature; coupled with morphological evidence, this supports a microbial origin of calcite crystals. SIMS δ13C data suggest that microbially mediated speleothem formation occurred repeatedly at short intervals before abiotic precipitation took over. The studied stalactite also contains iron and manganese oxides that have been mediated by microbial activity through extracellular polymeric substance (EPS)-influenced organomineralization processes. The latter reflect paleoenvironmental changes that occurred more than 500 000 yr ago, possibly related to the presence of a peat bog above the cave at that time. Microbial activity can initiate calcite deposition in the aphotic zone of caves before inorganic precipitation of speleothem carbonates. This study highlights the importance of microbially induced fractionation that can result in large negative δ13C excursions. The microscale biogeochemical processes imply that microbial activity has only negligible effects on the bulk δ13C signature in speleothems, which is more strongly affected by CO2 degassing and the host rock signature.

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Pacton, M., S. F. M. Breitenbach, F. A. Lechleitner, A. Vaks, C. Rollion-Bard, O. S. Gutareva, A. V. Osinzev, and C. Vasconcelos. "The role of microorganisms on the formation of a stalactite in Botovskaya Cave, Siberia – palaeoenvironmental implications." Biogeosciences Discussions 10, no. 4 (April 8, 2013): 6563–603. http://dx.doi.org/10.5194/bgd-10-6563-2013.

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Abstract. Calcitic speleothems in caves can form through abiogenic, biogenic, or a combination of both processes. Many issues conspire to make the assessment of biogenicity difficult, especially when focusing on old speleothem deposits. This study reports a multiproxy analysis of a Siberian stalactite, combining high-resolution microscopy, isotope geochemistry and microbially enhanced mineral precipitation laboratory experiments. The contact between growth layers in a stalactite exhibits a biogenic isotopic signature; coupled with morphological evidence this supports a microbial origin of calcite crystals. SIMS δ13C data suggest that microbially mediated speleothem formation occurred repeatedly for short intervals before abiotic precipitation took over. The studied stalactite also contains iron and manganese oxides that have been mediated by microbial activity through extracellular polymeric substances (EPS)-influenced organomineralization processes. The latter reflect palaeoenvironmental changes that occurred more than 500 000 yr ago, possibly related to the presence of a peat bog above the cave at that time. Microbial activity can initiate calcite deposition in the aphotic zone of caves before inorganic precipitation of speleothem carbonates. This study highlights the importance of microbially induced fractionation that can result in large negative δ13C excursions. The micro-scale biogeochemical processes imply that microbial activity has only negligible effects on the bulk δ13C signature in speleothems, which is more strongly affected by CO2 degassing and the hostrock signature.

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Kim, Yumi, and Yul Roh. "Microbially Induced Carbonate Precipitation Using Microorganisms Enriched from Calcareous Materials in Marine Environments and Their Metabolites." Minerals 9, no. 12 (November 21, 2019): 722. http://dx.doi.org/10.3390/min9120722.

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Microbially induced Ca-carbonate precipitation (MICP) in general, refers to a process in which the urease secreted by microbes hydrolyzes urea to ammonium and carbon dioxide. The main objectives of this study were to identify the environmental factors (e.g., microbial growth, cell/metabolite presences, and calcium sources) that control Ca-carbonate formation and to investigate the mineralogical characteristics of the Ca-carbonate precipitated using ureolytic microorganisms cultured in marine environments. The two types of carbonate-forming microorganisms (CFMs), mixed cultures hydrolyzing urea, were enriched from calcareous materials in marine environments. The experiments using a CFM, Sporosarcina pasteurii, was also used for comparison. All the microbes were cultured aerobically in D-1 growth media that included urea. To investigate the effect of microbial growth states on Ca-carbonate precipitation, Ca-acetate was injected into the media before (i.e., lag phase) and after (i.e., stationary phase) microbial growth, and into the soluble microbial products (SMP) solution, respectively. XRD, FT-IR, and SEM-EDS analyses were used for mineralogical characterization of the precipitated Ca-carbonates. Results indicated that the Ca-carbonates, vaterite and/or calcite, precipitated under all the experimental conditions. The fastest precipitation of Ca-carbonates occurred in the SMP solution and formed calcite (size = 5–15 μm). When the concentrations of added Ca-acetate were varied from 0 to 0.5 M, the highest amounts of calcite, 22.8 g/L, were produced when 0.3 M Ca-acetate was injected. Therefore, the environmental factors (e.g., microbial growth, cell/metabolite presences, and calcium sources) could have an effect the rate of formation of Ca-carbonate and the types of carbonate minerals formed. Moreover, the use of cell-free SMP solution is expected to be applicable to Ca-carbonate precipitation in an environment where microbial growth is unfavorable.

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Achal, Varenyam. "Bioremediation of Pb-Contaminated Soil Based on Microbially Induced Calcite Precipitation." Journal of Microbiology and Biotechnology 22, no. 2 (February 28, 2012): 244–47. http://dx.doi.org/10.4014/jmb.1108.08033.

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Hafez, Nehad M., Mohie Eldin Elmashad, and Abdullah Galaa. "A Model-Scale Investigation for Microbially Induced Calcite Precipitation in Sand." Journal of Civil Engineering and Construction 8, no. 4 (November 15, 2019): 149–56. http://dx.doi.org/10.32732/jcec.2019.8.4.149.

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New, exciting opportunities for utilizing biological processes to modify the engineering properties of the soil (e.g. strength, stiffness, permeability) have recently emerged. Enabled by interdisciplinary research at the confluence of microbiology, geochemistry, and civil engineering, this new field has the potential to meet society’s ever-expanding needs for innovative treatment processes that improve soil supporting new and existing infrastructure. Ureolytic bacteria are one of the most efficient organisms in producing amounts of carbonate that easily react with the free calcium ions available in the environment. Sporosarcina pasteurii, a robust microbial alkaline environment was used in this work for its high potential in the biocementation process that involves the biomediated calcite precipitation. This study presents the results of a model-scale laboratory investigation conducted on bio-cemented siliceous sand. Chemicals used in this study are commercially available in order investigate the viability of implementing this technique in the field at larger scales. To make it more practical, the microbial cells are directly used with neither sterilization nor utilization of a centrifuge process for the growth medium. Blocks of the bio-treated soil were excavated from the model and were tested to examine the strength and durability parameters of the improved soil. The results show that the unconfined compressive strength (UCS) and slake durability index significant increased upon biological treatment. However, due to the downwards permeation of the fluid due to gravity, samples obtained from the bottom and the center of the treated column gave larger UCS and slake durability indices than those obtained from the top and the sides of the column.

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Li, Mingdong, Kejun Wen, Yu Li, and Liping Zhu. "Impact of Oxygen Availability on Microbially Induced Calcite Precipitation (MICP) Treatment." Geomicrobiology Journal 35, no. 1 (May 11, 2017): 15–22. http://dx.doi.org/10.1080/01490451.2017.1303553.

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Mahawish, Aamir, Abdelmalek Bouazza, and Will P. Gates. "Improvement of Coarse Sand Engineering Properties by Microbially Induced Calcite Precipitation." Geomicrobiology Journal 35, no. 10 (September 11, 2018): 887–97. http://dx.doi.org/10.1080/01490451.2018.1488019.

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Montoya, B. M., and J. T. DeJong. "Stress-Strain Behavior of Sands Cemented by Microbially Induced Calcite Precipitation." Journal of Geotechnical and Geoenvironmental Engineering 141, no. 6 (June 2015): 04015019. http://dx.doi.org/10.1061/(asce)gt.1943-5606.0001302.

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Darby, Kathleen M., Gabby L. Hernandez, Jason T. DeJong, Ross W. Boulanger, Michael G. Gomez, and Daniel W. Wilson. "Centrifuge Model Testing of Liquefaction Mitigation via Microbially Induced Calcite Precipitation." Journal of Geotechnical and Geoenvironmental Engineering 145, no. 10 (October 2019): 04019084. http://dx.doi.org/10.1061/(asce)gt.1943-5606.0002122.

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Song, Chenpeng, and Derek Elsworth. "Microbially Induced Calcium Carbonate Plugging for Enhanced Oil Recovery." Geofluids 2020 (July 2, 2020): 1–10. http://dx.doi.org/10.1155/2020/5921789.

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Plugging high-permeability zones within oil reservoirs is a straightforward approach to enhance oil recovery by diverting waterflooding fluids through the lower-permeability oil-saturated zones and thereby increase hydrocarbon displacement by improvements in sweep efficiency. Sporosarcina pasteurii (ATCC 11859) is a nitrogen-circulating bacterium capable of precipitating calcium carbonate given a calcium ion source and urea. This microbially induced carbonate precipitation (MICP) is able to infill the pore spaces of the porous medium and thus can act as a potential microbial plugging agent for enhancing sweep efficiency. The following explores the microscopic characteristics of MICP-plugging and its effectiveness in permeability reduction. We fabricate artificial rock cores composed of Ottawa sand with three separate grain-size fractions which represent large (40/60 mesh sand), intermediate (60/80 mesh sand), and small (80/120 mesh sand) pore sizes. The results indicate a significant reduction in permeability after only short periods of MICP treatment. Specifically, after eight cycles of microbial treatment (about four days), the permeability for the artificial cores representing large, intermediate, and small pore size maximally drop to 47%, 32%, and 16% of individual initial permeabilities. X-ray diffraction (XRD) indicates that most of the generated calcium carbonate crystals occur as vaterite with only a small amount of calcite. Imaging by SEM indicates that the pore wall is coated by a calcium carbonate film with crystals of vaterite and calcite scattered on the pore wall and acting to effectively plug the pore space. The distribution pattern and morphology of microbially mediated CaCO3 indicate that MICP has a higher efficiency in plugging pores compared with extracellular polymeric substances (EPSs) which are currently the primary microbial plugging agent used to enhance sweep efficiency.

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Khan, MNH, S. Kawasaki, and MR Hassan. "Sand Solidification through Microbially Induced Carbonate Precipitation for Erosion Control: Prospects in Bangladesh." Journal of Environmental Science and Natural Resources 9, no. 1 (November 8, 2016): 59–61. http://dx.doi.org/10.3329/jesnr.v9i1.30292.

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Bio-cementation is a sand consolidation technology, in which ureolytic bacteria release carbonate from urea hydrolysis in the presence of an excess of calcium ions to form calcite (CaCO3) in-situ. Biocementation is to enhance the strength and stiffness properties of soil and rocks though microbial activity or products. This paper addressed the prospect of microbial carbonate precipitation for erosion control in Bangladesh. Bacterial CaCO3 precipitation under appropriate conditions is a general phenomenon where the ureolytic bacteria uses urea as an energy source and produces ammonia which increases the pH in the environment and generates carbonate, causing Ca2+ and CO32- to be precipitated as CaCO3. This CaCO3 join sand particles and forms rocklike materials that auto-repairs by means of sunlight, seawater, and bacteria as microbially induced carbonate precipitation method. These rock particles when produced artificially is called artificial rock and has the potentiality to protect coastlines from erosion.J. Environ. Sci. & Natural Resources, 9(1): 59-61 2016

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Indriani, Andi Marini, and Gunaedy Utomo. "Pengaruh Microbially Induced Calcite Precipitation (MICP) terhadap Perilaku Kuat Geser Tanah Terkontaminasi Batubara." CIVED 10, no. 1 (March 25, 2023): 53. http://dx.doi.org/10.24036/cived.v10i1.122318.

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Microbially induced calcite precipitation (MICP) adalah teknik perbaikan tanah dengan menggunakan mikroorganisme yang mampu mengubah dan meningkatkan sifat mekanik dan fisik. Dalam penelitian ini, uji geser langsung dengan mengacu pada standard SNI 03-3420-1994 digunakan untuk mengetahui pengaruh pengendapan calcite terhadap perilaku kuat geser tanah terkontaminasi batubara. Bakteri Bacillus subtilis sebanyak 6% ditambahkan ke dalam tanah yang terkontaminasi 5%, 10% dan 15% batubara. Bakteri yang digunakan menggunakan kultur 3 hari dimana berada pada fase stasioner. Hasil penelitian menunjukkan bahwa terjadi peningkatan yang cukup baik terhadap nilai kohesi dan sudut geser dalam sebagai parameter kuat geser setelah masa pemeraman. Stabilisasi MICP pada tanah terkontaminasi 5% batubara meningkatkan kuat geser sebesar 3 kali lipat sedangkan pada tanah terkontaminasi 10% dan 15% batubara terjadi peningkatan kuat geser masing-masing sebesar 7 dan 15 kali lipat dibandingkan dengan tanah asli.

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Xiao, J. Z., Y. Q. Wei, H. Cai, Z. W. Wang, T. Yang, Q. H. Wang, and S. F. Wu. "Microbial-Induced Carbonate Precipitation for Strengthening Soft Clay." Advances in Materials Science and Engineering 2020 (April 14, 2020): 1–11. http://dx.doi.org/10.1155/2020/8140724.

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Currently, calcite produced in sediments by microbial-induced carbonate precipitation (MICP) is mainly used as a strengthening binder in sand because sands are porous and have good permeability. Conventional wisdom does not consider MICP to be suitable for use in soft clay because of the clay particles’ small size and its minimal porosity. Because of the clay’s high water content and complex chemical composition, very little research has been done and not much is known about the use of MICP in soft clay for strength enhancement. For this paper, soft clay specimens were prepared by mixing a solution containing Sporosarcina pasteurii bacteria, solutions with different concentrations of nutrient salts, and soft clay. Unconfined compressive strength tests were carried out on these specimens after they had cured for 28 days in a moisture-controlled environment. These laboratory tests were used to study the chemical reactions, the clay’s strength, and other influencing factors. The results are as follows: (1) directly mixing a S. pasteurii solution, nutrient salts, and soft clay considerably improves the uniformity of the spatial distribution of the bacteria and the nutrients in the soft clay. Directly mixing these constituents promotes the formation of calcium carbonate and greatly simplifies soft clay sample preparation. (2) It is feasible to use MICP to increase the strength of soft clay. Compared to control specimens cured under the same conditions but without introduced nutrients and bacteria solution, the unconfined compressive strength of MICP-treated specimens can be increased by as much as 2.42 times to an unconfined compressive strength of 43.31 kPa. The water content in MICP-treated specimens was significantly reduced by the MICP reactions and in one case decreased from 40% to 30.73%. (3) The strength enhancement of microbially solidified soft clay is the result of two processes: urea hydration catalyzed by enzymes consumes water in the clay and the bacterially precipitated calcite forms in the sediment’s pores. (4) The micro-organism-produced calcite in the soft clay increases the calcite abundance from 0% to as much as 3.5%. (5) The MICP-treated strength of soft clay varies with the concentration of the nutrients provided. For the experimental conditions used for this paper, the optimum concentration of the CaCl2·2H2O and CH4N2O nutrients is 0.5 mol/L.

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Landa-Marbán, D., S. Tveit, K. Kumar, and S. E. Gasda. "Practical approaches to study microbially induced calcite precipitation at the field scale." International Journal of Greenhouse Gas Control 106 (March 2021): 103256. http://dx.doi.org/10.1016/j.ijggc.2021.103256.

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Gebru, Kbrom Alebel, Tekleweyni Gebremicael Kidanemariam, and Haile Kidane Gebretinsae. "Bio-cement production using microbially induced calcite precipitation (MICP) method: A review." Chemical Engineering Science 238 (July 2021): 116610. http://dx.doi.org/10.1016/j.ces.2021.116610.

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Arpajirakul, Soyson, Wiboonluk Pungrasmi, and Suched Likitlersuang. "Efficiency of microbially-induced calcite precipitation in natural clays for ground improvement." Construction and Building Materials 282 (May 2021): 122722. http://dx.doi.org/10.1016/j.conbuildmat.2021.122722.

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Kang, Chang-Ho, Yoon-Jung Kwon, and Jae-Seong So. "Soil Bioconsolidation Through Microbially Induced Calcite Precipitation by Lysinibacillus sphaericus WJ-8." Geomicrobiology Journal 33, no. 6 (February 26, 2016): 473–78. http://dx.doi.org/10.1080/01490451.2015.1053581.

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Martinez, Alejandro, Lin Huang, and Michael G. Gomez. "Enhancement of the thermal conductivity of sands via microbially-induced calcite precipitation." E3S Web of Conferences 205 (2020): 09011. http://dx.doi.org/10.1051/e3sconf/202020509011.

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Energy piles and ground source heat pump systems have been shown to provide sustainable alternatives for temperature regulation in buildings and other applications such as road de-icing. However, their efficiency can be undermined in partially-saturated and dry sandy soils due to the relatively low thermal conductivity (kt) of these materials. Microbially-Induced Calcite Precipitation (MICP) has been demonstrated to be an environmentally-conscious ground improvement technology capable of modifying the engineering properties of sandy soils including increases in shear stiffness and strength and decreases in hydraulic conductivity. These improvements result from the precipitation of calcium carbonate crystals at inter-particle contacts and on particle surfaces. This paper presents results from a soil column study aimed at investigating changes in soil kt during MICP treatments and subsequent desaturation using a poorly- graded sand. The results indicate that while bio-cementation can increase soil kt, the level of enhancement depends on the degree of saturation. For instance, increases of up to 330% were measured under dry conditions while only modest increases of about 15% were measured under saturated conditions. MICP treatment may therefore be most effective at enhancing the kt of partially-saturated and dry sands. In addition, the similarity between the evolution of kt and shear wave velocity (Vs) during MICP treatment suggests that kt may provide a new method to assess cementation level and contact quality.

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Hommel, Johannes, Alfred B. Cunningham, Rainer Helmig, Anozie Ebigbo, and Holger Class. "Numerical Investigation of Microbially Induced Calcite Precipitation as a Leakage Mitigation Technology." Energy Procedia 40 (2013): 392–97. http://dx.doi.org/10.1016/j.egypro.2013.08.045.

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Duo, Li, Tian Kan-liang, Zhang Hui-li, Wu Yu-yao, Nie Kang-yi, and Zhang Shi-can. "Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation." Construction and Building Materials 172 (May 2018): 251–62. http://dx.doi.org/10.1016/j.conbuildmat.2018.03.255.

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Akiyama, Masaru, and Satoru Kawasaki. "Biogeochemical simulation of microbially induced calcite precipitation with Pararhodobacter sp. strain SO1." Acta Geotechnica 14, no. 3 (March 6, 2019): 685–96. http://dx.doi.org/10.1007/s11440-019-00784-z.

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GOMAA, EMAN ZAKARIA. "Biosequestration of heavy metals by microbially induced calcite precipitation of ureolytic bacteria." ROMANIAN BIOTECHNOLOGICAL LETTERS 24, no. 1 (February 28, 2019): 147–53. http://dx.doi.org/10.25083/rbl/24.1/147.153.

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Choi, Sun-Gyu, Tung Hoang, and Sung-Sik Park. "Undrained Behavior of Microbially Induced Calcite Precipitated Sand with Polyvinyl Alcohol Fiber." Applied Sciences 9, no. 6 (March 22, 2019): 1214. http://dx.doi.org/10.3390/app9061214.

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Microbially induced calcite precipitation can cement sand and is an environment-friendly alternative to ordinary Portland cement. In this study, clean Ottawa sand was microbially treated to induce calcite contents (CCs) of 0%, 2%, and 4%. Polyvinyl alcohol fiber was also mixed with the sand at four different contents (0%, 0.2%, 0.4%, and 0.6%) with a constant CC of 4%. A series of undrained triaxial tests was conducted on the treated sands to evaluate the effects of the calcite treatment and fiber inclusion. Their hydraulic conductivity was also determined using a constant head test. As the CC increased from 0% to 4%, the friction angle and cohesion increased from 35.3° to 39.6° and from 0 to 93 kPa, respectively. For specimens with a CC of 4%, as the fiber content increased from 0% to 0.6%, the friction angle and cohesion increased from 39.6° to 42.8° and from 93 to 139 kPa, respectively. The hydraulic conductivity of clean Ottawa sand decreased by a factor of more than 100 as the CC increased from 0% to 4%. The fiber inclusion had less effect on the hydraulic conductivity of the specimen with 4% CC.

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Riveros, Guillermo Alexander, and Abouzar Sadrekarimi. "Effect of microbially induced cementation on the instability and critical state behaviours of Fraser River sand." Canadian Geotechnical Journal 57, no. 12 (December 2020): 1870–80. http://dx.doi.org/10.1139/cgj-2019-0514.

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Microbially induced calcite precipitation (MICP) is a naturally driven biological process that harnesses the natural metabolic action of bacteria to induce the precipitation of calcium carbonate and alter soil engineering properties. This paper presents the results of using MICP to improve the monotonic undrained yield and critical strengths of Fraser River sand specimens. Bacteria called “Sporosarcina ureae” are employed as a ureolytic organism to achieve MICP. The formation of calcite cementation among sand particles is confirmed using scanning electron microscopic images and X-ray compositional analysis of cemented sand clusters. The progress of MICP cementation is assessed by measuring the velocity of a shear wave (VS) traveling through the specimen. The results show that VS starts to increase just as the calcium solution is introduced into each specimen after soaking the samples with the bacterial solution. Improvement in monotonic strength of sand samples is subsequently measured in a series of direct simple shear tests. Due to the combined effects of particle cementation and densification, the sand’s undrained and drained monotonic shearing strengths are significantly enhanced.

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Rajasekar, Adharsh, Charles K. S. Moy, Stephen Wilkinson, and Raju Sekar. "Microbially induced calcite precipitation performance of multiple landfill indigenous bacteria compared to a commercially available bacteria in porous media." PLOS ONE 16, no. 7 (July 16, 2021): e0254676. http://dx.doi.org/10.1371/journal.pone.0254676.

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Microbially Induced Carbonate Precipitation (MICP) is currently viewed as one of the potential prominent processes for field applications towards the prevention of soil erosion, healing cracks in bricks, and groundwater contamination. Typically, the bacteria involved in MICP manipulate their environment leading to calcite precipitation with an enzyme such as urease, causing calcite crystals to form on the surface of grains forming cementation bonds between particles that help in reducing soil permeability and increase overall compressive strength. In this paper, the main focus is to study the MICP performance of three indigenous landfill bacteria against a well-known commercially bought MICP bacteria (Bacillus megaterium) using sand columns. In order to check the viability of the method for potential field conditions, the tests were carried out at slightly less favourable environmental conditions, i.e., at temperatures between 15-17°C and without the addition of urease enzymes. Furthermore, the sand was loose without any compaction to imitate real ground conditions. The results showed that the indigenous bacteria yielded similar permeability reduction (4.79 E-05 to 5.65 E-05) and calcium carbonate formation (14.4–14.7%) to the control bacteria (Bacillus megaterium), which had permeability reduction of 4.56 E-5 and CaCO3 of 13.6%. Also, reasonably good unconfined compressive strengths (160–258 kPa) were noted for the indigenous bacteria samples (160 kPa). SEM and XRD showed the variation of biocrystals formation mainly detected as Calcite and Vaterite. Overall, all of the indigenous bacteria performed slightly better than the control bacteria in strength, permeability, and CaCO3 precipitation. In retrospect, this study provides clear evidence that the indigenous bacteria in such environments can provide similar calcite precipitation potential as well-documented bacteria from cell culture banks. Hence, the idea of MICP field application through biostimulation of indigenous bacteria rather than bioaugmentation can become a reality in the near future.

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Yun, SeongYeol, WooRi Cho, SeungJin Oh, Minah Oh, and Jai-Young Lee. "A study on Recycling of Waste Gypsum Using Microbially Induced Calcite Precipitation (MICP)." Journal of the Korean Society of Urban Environment 21, no. 1 (March 31, 2021): 21–29. http://dx.doi.org/10.33768/ksue.2021.21.1.021.

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Cheng, Liang, and Ralf Cord-Ruwisch. "Upscaling Effects of Soil Improvement by Microbially Induced Calcite Precipitation by Surface Percolation." Geomicrobiology Journal 31, no. 5 (March 4, 2014): 396–406. http://dx.doi.org/10.1080/01490451.2013.836579.

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Jiang, Ning-Jun, and Kenichi Soga. "Erosional behavior of gravel-sand mixtures stabilized by microbially induced calcite precipitation (MICP)." Soils and Foundations 59, no. 3 (June 2019): 699–709. http://dx.doi.org/10.1016/j.sandf.2019.02.003.

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Al-Salloum, Yousef, H. Abbas, Q. I. Sheikh, S. Hadi, Saleh Alsayed, and Tarek Almusallam. "Effect of some biotic factors on microbially-induced calcite precipitation in cement mortar." Saudi Journal of Biological Sciences 24, no. 2 (February 2017): 286–94. http://dx.doi.org/10.1016/j.sjbs.2016.01.016.

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Journal articles on the topic 'Microbially Induced Calcite Precipitation'

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