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Journal articles on the topic 'Microbiological assay'

Author: Grafiati

Published: 4 June 2021

Last updated: 27 February 2023

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1

Keagy, Pamela M. "Computerized Semiautomated Microbiological Assay of Folacin." Journal of AOAC INTERNATIONAL 69, no. 5 (1986): 773–77. http://dx.doi.org/10.1093/jaoac/69.5.773.

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Abstract A semiautomated microbiological folacin assay system is described. microcomputer A controls sample dilutions, medium addition, turbidity determination, and data acquisition. Assay capacity is 600 tubes per day, approximately twice that of comparable manual assays. Using the automated equipment, more samples can be compared within one assay, eliminating many sources of between-assay variation in large studies. Additional advantages of this sytem are reduced human errors, flexibility of assay design, and multifunctional component equipment. Folacin results from chicken liver, spinach, a

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2

Bergan, Tom, and Bjorg Øydvin. "MICROBIOLOGICAL ASSAY OF JOSAMYCIN." Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology 80B, no. 1 (2009): 101–6. http://dx.doi.org/10.1111/j.1699-0463.1972.tb00134.x.

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3

Moreno, Andréia De Haro, and Hérida Regina Nunes Salgado. "Microbiological Assay for Ceftazidime Injection." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1379–82. http://dx.doi.org/10.1093/jaoac/90.5.1379.

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Abstract A simple, sensitive, and specific biodiffusion assay for the antibacterial ceftazidime was developed using a strain of Staphylococcus epidermidis (ATCC 12228) as the test organism. Ceftazidime was measured in powder for injection at concentrations ranging from 100 to 400 g/mL. The calibration graph for ceftazidime was linear (r2 = 1), and the method validation showed that it was precise (relative standard deviation = 0.415) and accurate. The results obtained by biodiffusion assay were statistically calculated by linear parallel model and by means of regression analysis and were verifi

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4

Singer, Carol J., and Stanley E. Katz. "Microbiological Assay for Chloramphenicol Residues." Journal of AOAC INTERNATIONAL 68, no. 5 (1985): 1037–41. http://dx.doi.org/10.1093/jaoac/68.5.1037.

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Abstract Procedures for the assay of chloramphenicol in milk, urine, serum, and muscle tissue are presented. The procedures specify an assay design with all standards as well as samples present on each plate, oxytetracycline in the buffer-diluent for greater sensitivity, a minimal medium to enhance the inhibitory effect of chloramphenicol on the assay organism, and a tetrazolium dye to improve the ability to measure the zones of inhibition. Recoveries of unbound chloramphenicol from bovine urine were 90.8%, from serum 88.3%, from milk 79.3%, from swine muscle 71.3%, and from beef and chicken m

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5

Souza, Marinês J. e., Celso F. Bittencourt, and Paulo da S. e. Souza Filho. "Microbiological assay for enrofloxacin injection." International Journal of Pharmaceutics 271, no. 1-2 (2004): 287–91. http://dx.doi.org/10.1016/j.ijpharm.2003.11.012.

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6

Hasselberger, Mary Lee. "Assay of Oxytetracycline in Animal Feed by Liquid Chromatography and Microbiological Plate Assay." Journal of AOAC INTERNATIONAL 76, no. 1 (1993): 39–43. http://dx.doi.org/10.1093/jaoac/76.1.39.

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Abstract In a proposed method for determining oxytetracycline (OTC) in animal feed, OTC is extracted with acidic methanol, and the extract is centrifuged and assayed by microbiological plate assay and by reversed- phase liquid chromatography (LC). Riboflavin and furazolidone elute at retention times similar to that of OTC. Successful separations are made by using both dimethylformamide and acetonitrile mobile phases. Variation between microbiological and LC results is comparable with variation within the microbiological assay. The relative standard deviation for 6 replicates of feed at the 100

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7

Zhuang, David, Melinda Smedema, Ann LeMonte, Benita K. Book, Mark D. Pescovitz, and L. Joseph Wheat. "Effect of Calcineurin Inhibitors on Posaconazole Blood Levels as Measured by the MVista Microbiological Assay." Antimicrobial Agents and Chemotherapy 52, no. 2 (2007): 730–31. http://dx.doi.org/10.1128/aac.01096-07.

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ABSTRACT Calcineurin inhibitors may augment the effects of antifungal drugs in microbiological assays. We examined this interaction in a microbiological assay for posaconazole. No effect was observed. However, concurrent or recently discontinued treatment with other antifungal drugs caused false-positive results, emphasizing a limitation of microbiological assays for antifungal drug level measurement.

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8

Adams, E., L. Liu, K. Dierick, et al. "Neomycin: microbiological assay or liquid chromatography?" Journal of Pharmaceutical and Biomedical Analysis 17, no. 4-5 (1998): 757–66. http://dx.doi.org/10.1016/s0731-7085(97)00247-1.

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9

Staub, Inara, Elfrides E. S. Schapoval, and Ana M. Bergold. "Microbiological assay of ketoconazole in shampoo." International Journal of Pharmaceutics 292, no. 1-2 (2005): 195–99. http://dx.doi.org/10.1016/j.ijpharm.2004.12.001.

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10

Horie, Masakazu, Harumi Kobayashi, Rie Ishii, and Hiroyuki Nakazawa. "Sensitive Microbiological Assay of Residual Antibacterials in Meat by Microbiological Method." BUNSEKI KAGAKU 56, no. 12 (2007): 1097–103. http://dx.doi.org/10.2116/bunsekikagaku.56.1097.

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11

Guilarte, Tomas R. "Radiometric microbiological assay of B vitamins. part 1: assay procedure." Journal of Nutritional Biochemistry 2, no. 6 (1991): 334–38. http://dx.doi.org/10.1016/0955-2863(91)90079-k.

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12

Loureno, Felipe Rebello, Telma Mary Kaneko, and Terezinha De Jesus Andreoli Pinto. "Validation of Erythromycin Microbiological Assay Using an Alternative Experimental Design." Journal of AOAC INTERNATIONAL 90, no. 4 (2007): 1107–10. http://dx.doi.org/10.1093/jaoac/90.4.1107.

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Abstract The agar diffusion method, widely used in antibiotic dosage, relates the diameter of the inhibition zone to the dose of the substance assayed. An experimental plan is proposed that may provide better results and an indication of the assay validity. The symmetric or balanced assays (2 2) as well as those with interpolation in standard curve (5 1) are the main designs used in the dosage of antibiotics. This study proposes an alternative experimental design for erythromycin microbiological assay with the evaluation of the validation parameters of the method referring to linearity, precis

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13

Kochansky, Jan, Mark F. Feldlaufer, and I. Barton Smith. "Microbiological screening assay for tylosin in pollen." Journal of Apicultural Research 45, no. 2 (2006): 37–41. http://dx.doi.org/10.1080/00218839.2006.11101323.

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14

Gomes, Greici Cristiani, and Hérida Regina Nunes Salgado. "Microbiological Assay for Lomefloxacin in Coated Tablets." Journal of AOAC INTERNATIONAL 89, no. 4 (2006): 1077–79. http://dx.doi.org/10.1093/jaoac/89.4.1077.

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Abstract The validation of a microbiological assay, applying cylinder plate method for determination of the activity of lomefloxacin in coated tablets is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, lomefloxacin was measured in concentrations ranging from 2.0 to 8.0 μg/mL. The method validation showed that it is linear (r = 0.9999), precise (relative standard deviation = 1.15%), and accurate (it measured the added quantities). The excipients did not interfere in the determination. It was concluded that the microbiological assay is satisfactory for quantitation

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15

Salgado, H. R. N., C. C. G. O. Lopes, and M. B. B. Lucchesi. "Microbiological assay for gatifloxacin in pharmaceutical formulations." Journal of Pharmaceutical and Biomedical Analysis 40, no. 2 (2006): 443–46. http://dx.doi.org/10.1016/j.jpba.2005.07.020.

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16

da Silveira Ev, Lisiane, and Elfrides E. S. Schapoval. "Microbiological assay for determination of ofloxacin injection." Journal of Pharmaceutical and Biomedical Analysis 27, no. 1-2 (2002): 91–96. http://dx.doi.org/10.1016/s0731-7085(01)00513-1.

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17

Breier, A. R., C. V. Garcia, T. P. Oppe, M. Steppe, and E. E. S. Schapoval. "Microbiological assay for azithromycin in pharmaceutical formulations." Journal of Pharmaceutical and Biomedical Analysis 29, no. 5 (2002): 957–61. http://dx.doi.org/10.1016/s0731-7085(02)00213-3.

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18

Thickett, K. J., and T. G. Winstanley. "Multipoint microbiological assay for detecting beta-lactamase." Journal of Clinical Pathology 44, no. 5 (1991): 435–36. http://dx.doi.org/10.1136/jcp.44.5.435.

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19

JAKŠIĆ, S., I. JAJIĆ, S. KRSTOVIĆ, and Ž. MIHALJEV. "L-lysine determination in animal feed using microbiological microtiter plate assay." Journal of the Hellenic Veterinary Medical Society 69, no. 3 (2018): 1094. http://dx.doi.org/10.12681/jhvms.18881.

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Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such a

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20

Tarka, Patryk, and Aneta Nitsch-Osuch. "No-Touch Automated Disinfection System for Decontamination of Surfaces in Hospitals." International Journal of Environmental Research and Public Health 17, no. 14 (2020): 5131. http://dx.doi.org/10.3390/ijerph17145131.

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Background: Hospital-acquired infections (HAIs) remain a common problem, which suggests that standard decontamination procedures are insufficient. Thus, new methods of decontamination are needed in hospitals. Methods: We assessed the effectiveness of a no-touch automated disinfection (NTD) system in the decontamination of 50 surfaces in 10 hospital rooms. Contamination of surfaces was assessed with a microbiological assay and an ATP bioluminescence assay. Unacceptable contamination was defined as > 100 colony forming units/100 cm2 in the microbiological assay, and as ≥ 250 relative light un

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21

Thomas, Adrian H. "Replacement of microbiological assay by high-performance liquid chromatographic assay for antibiotics." Journal of Pharmaceutical and Biomedical Analysis 5, no. 4 (1987): 319–24. http://dx.doi.org/10.1016/0731-7085(87)80037-7.

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22

Carlos, Gerard M., Stewart B. Telfer, C. Lewis Johnson, D. Ian Givens, Roger J. Wilkins, and Robert D. Newberry. "Microbiological assay of blood serum for the vitamin B12 status of dairy cows." Journal of Dairy Research 54, no. 4 (1987): 463–70. http://dx.doi.org/10.1017/s0022029900025668.

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SummaryFour vitamin B12 assays were compared using blood sera from Friesian cows on winter diets or grazing. In herd 1, ten animals were blood-sampled three times at monthly intervals and the vitamin B12 concentration of the sera determined by the Poteriochromonas malhamensis and Lactobacillus delbrueckii assays. At all three sampling dates the results produced by the P. malhamensis assay were significantly greater than those produced by the L. delbrueckii assay. Cows in herd 2 were divided into two groups, each of 30 animals. One group was given a soluble glass bolus releasing cobalt and the

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23

Calixto, Giovana, Luciana Domingos, Nathalie Fabri, et al. "Microbiological Assay for the Determination of Colistin Sulphate." Current Analytical Chemistry 10, no. 3 (2014): 443–48. http://dx.doi.org/10.2174/15734110113090990017.

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24

Greenwood, David. "Book Review: Theory and Application of Microbiological Assay." Alternatives to Laboratory Animals 19, no. 1 (1991): 153–54. http://dx.doi.org/10.1177/026119299101900134.

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25

Nunes Salgado, Hrida Regina, and Greici Cristiani Gomes Tozo. "Microbiological Assay for Cefoxitin Sodium in Dosage Form." Journal of AOAC INTERNATIONAL 90, no. 2 (2007): 452–55. http://dx.doi.org/10.1093/jaoac/90.2.452.

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Abstract A microbiological assay applying the cylinder-plate method is described for determination of the activity of cefoxitin sodium in injectables. Using a strain of Staphylococcus epidermidis ATCC 12226 as the test organism, cefoxitin sodium was measured in concentrations ranging from 50.0 to 200.0 g/mL. The validation showed that the method was linear (r = 0.9998), precise (RSD = 0.81%), and accurate. It was concluded that the microbiological assay is satisfactory for quantitation of cefoxitin sodium in injectables.

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26

Shaw, W. H., and R. E. Duncombe. "CONTINUOUS AUTOMATIC MICROBIOLOGICAL ASSAY OF ANTIBIOTICS. PART II." Annals of the New York Academy of Sciences 130, no. 2 (2006): 647–56. http://dx.doi.org/10.1111/j.1749-6632.1965.tb12608.x.

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27

GUILARTE, TOMAS R. "Radiometric-Microbiological Assay of Biotin in Human Plasma." Annals of the New York Academy of Sciences 447, no. 1 Biotin (1985): 398–99. http://dx.doi.org/10.1111/j.1749-6632.1985.tb18457.x.

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28

Sun, Li, Wenqing Chen, Zixin Deng, and Jian-Jiang Zhong. "Microbiological assay for quantitative determination of polyoxin B." Process Biochemistry 44, no. 3 (2009): 361–64. http://dx.doi.org/10.1016/j.procbio.2008.11.011.

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29

Horne, D. W., and D. Patterson. "Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates." Clinical Chemistry 34, no. 11 (1988): 2357–59. http://dx.doi.org/10.1093/clinchem/34.11.2357.

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Abstract Microbiological assay is still widely used for estimating folic acid derivatives in serum and other biological samples. We describe here a modification of this procedure involving use of 96-well microtiter plates. This procedure, used with modern, computer-interfaced microtiter-plate readers and data-reduction software, greatly shortens the time and minimizes reagent costs for this assay. Under the conditions of our assay procedures, all folic acid derivatives tested gave equal growth response for Lactobacillus casei. Results for assays of rat liver extracts showed excellent agreement

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30

Vaucher, Lauren C., Ana R. Breier, and Elfrides E. S. Schapoval. "Microbiological Assay for the Determination of Telithromycin in Tablets." Journal of AOAC INTERNATIONAL 89, no. 5 (2006): 1398–402. http://dx.doi.org/10.1093/jaoac/89.5.1398.

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Abstract This study describes the development and validation of a microbiological assay, applying the cylinder-plate method, for the determination of the antibiotic telithromycin. The microbiological method consisted of a cylinder-plate agar diffusion assay using Micrococcus luteus ATCC 9341 as the test microorganism. The response graphs for standard and sample solutions were linear (r = 0.9987), and no parallelism deviations were detected in the tested concentrations (0.25, 0.5, and 1.0 g/mL). The interday precision was 2.67%. Recovery values were between 96.75 and 100.91%. A preliminary stab

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31

Sheridan, B. L., and L. C. Pearce. "Vitamin B12 assays compared by use of patients' sera with low vitamin B12 content." Clinical Chemistry 31, no. 5 (1985): 734–36. http://dx.doi.org/10.1093/clinchem/31.5.734.

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Abstract We compared four radioisotope dilution (RD) methods and a microbiological assay for measuring concentrations of vitamin B12 in a selected panel of serum samples from patients known to be deficient in the vitamin. Low (less than 100 ng/L) and borderline (100-180 ng/L) results were similar between methods, but use of the manufacturers' recommended ranges for borderline results would have changed the diagnostic classifications for 22 of 38 samples. Results of all the RD methods inter-correlated well, but less so with the microbiological assay. Borderline, nondiagnostic results were commo

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32

Cendejas-Bueno, Emilio, Manuel Cuenca-Estrella, and Alicia Gomez-Lopez. "Determination of Voriconazole Serum Concentration by Bioassay, a Valid Method for Therapeutic Drug Monitoring for Clinical Laboratories." Antimicrobial Agents and Chemotherapy 57, no. 7 (2013): 3437–40. http://dx.doi.org/10.1128/aac.00323-13.

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ABSTRACTWe describe here a simple, fast, and reliable bioassay method for therapeutic drug monitoring of voriconazole. Fifty-eight clinical and external quality control samples were evaluated with this microbiological assay, and results were compared with those obtained with a previously validated chromatographic method. A good correlation between both assays was observed. This particular microbiological method was demonstrated to be simple and offers enough precision and accuracy to perform voriconazole therapeutic drug monitoring in laboratories without specialized equipment.

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33

Lourenço, Felipe Rebello, Telma Mary Kaneko, and Terezinha De Jesus Andreoli Pinto. "Evaluating Measurement Uncertainty in the Microbiological Assay of Vancomycin from Methodology Validation Data." Journal of AOAC INTERNATIONAL 90, no. 5 (2007): 1383–86. http://dx.doi.org/10.1093/jaoac/90.5.1383.

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Abstract Methodology validation and measurement uncertainty estimation are fundamental to obtain reliable results. The microbiological methods are widely used to determine antibiotic assay, as they permit evaluation of the analyzed antibiotic activity. A microbiological assay of vancomycin was performed with adoption of experimental design 5 1 (interpolation in 5-point standard curve assay) with final concentrations from 6.4 to 15.6 g/mL (standards) and 10.0 g/mL (sample). Bacillus subtitlis (ATCC 6633) was the microorganism used, with antibiotic medium No. 8 as base layer and inoculated layer

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34

Anderson, Ellen M. "Turbidimetric Microbiological Assay Results Calculated by a BASIC Computer Program." Journal of AOAC INTERNATIONAL 72, no. 6 (1989): 950–52. http://dx.doi.org/10.1093/jaoac/72.6.950.

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Abstract A BASIC computer program was used to calculate the results of microbiological vitamin assays. The program, which incorporates the official AOAC guidelines for microbiological methods, reduces the uncertainty inherent in manual curve plotting and interpolation and minimizes the human error of repetitive calculations. Because the BASIC programming language is well suited for use on self-contained personal computers, it can be easily adapted by small laboratories

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35

Kelleher, B. P., K. G. Walshe, J. M. Scott, and S. D. O'Broin. "Microbiological assay for vitamin B12 with use of a colistin-sulfate-resistant organism." Clinical Chemistry 33, no. 1 (1987): 52–54. http://dx.doi.org/10.1093/clinchem/33.1.52.

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Abstract In this simplified microbiological assay for serum vitamin B12, Lactobacillus leichmanii (NCIB 8117) adapted to tolerate high concentrations (500 mg/L) of the polymyxin antibiotic colistin sulfate is used. Results were similar in parallel experiments in which we used both the parent strain of L. leichmanii (NCIB 8117), and the new adapted strain. Evaluation of assay performance showed excellent analytical recovery of added cyanocobalamin (97%, SD 3%) and good interassay and intra-assay precision (CV less than 5%). This modified assay system obviates the need to sterilize culture mediu

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36

BAUTISTA, DERRICK A., JEAN PIERRE VAILLANCOURT, ROBERT A. CLARKE, SHANE RENWICK, and MANSEL W. GRIFFITHS. "Rapid Assessment of the Microbiological Quality of Poultry Carcasses Using ATP Bioluminescence." Journal of Food Protection 58, no. 5 (1995): 551–54. http://dx.doi.org/10.4315/0362-028x-58.5.551.

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The meat industry is in need of faster and more reliable methods to determine microbial loads in food products. A rapid method (&lt;15 min) has been developed to assess the microbiological quality of chicken carcasses using the adenosine triphosphate (ATP) bioluminescence assay. The results indicate that, following modifications, the ATP bioluminescence test produced an acceptable correlation with plate counts (r = 0.85, p &lt; 0.001) and demonstrated good repeatability between replicates. It is envisaged that the modified ATP bioluminescence assay would best be used as a platform reje

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37

Sung, Ji-Hee, Hyun-Hwa Cha, Nan-Young Lee, et al. "Diagnostic Accuracy of Loop-Mediated Isothermal Amplification Assay for Group B Streptococcus Detection in Recto-Vaginal Swab: Comparison with Polymerase Chain Reaction Test and Conventional Culture." Diagnostics 12, no. 7 (2022): 1569. http://dx.doi.org/10.3390/diagnostics12071569.

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A rapid method for obtaining group B streptococcus (GBS) screening results has been required in the obstetric field. We aimed to determine the diagnostic performance of the Loop-Mediated Isothermal Amplification (LAMP) assay is acceptable compared to the existing polymerase chain reaction (PCR) assay. The study involved 527 pregnant women aged 19 to 44 years. Rectovaginal swabs were collected between 35 and 37 weeks of gestation or prior to impending preterm births or term labor without GBS screening. We presented the diagnostic performance of the LAMP assay with a 95% confidence interval (CI)

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38

Schmidt, Martin, Martin Grey, and Martin Brendel. "A Microbiological Assay for the Quantitative Determination of Glutathione." BioTechniques 21, no. 5 (1996): 881–86. http://dx.doi.org/10.2144/96215st07.

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39

Albadawy, Omnia, Amany Nafee, Momen Thabet, Mostafa El-Rehewy, and Ahmed Ahmed. "MICROBIOLOGICAL ASSAY OF COLISTIN SULFATE ANTIBIOTIC IN PHARMACEUTICAL FORMULATIONS." Bulletin of Pharmaceutical Sciences. Assiut 36, no. 2 (2013): 99–103. http://dx.doi.org/10.21608/bfsa.2013.63200.

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40

Ciufetti, L. M., O. C. Yoder, and B. G. Turgeon. "A microbiological assay for host-specific fungal polyketide toxins." Fungal Genetics Reports 39, no. 1 (1992): 18–19. http://dx.doi.org/10.4148/1941-4765.1432.

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41

Miller, B. L., and R. D. Wyatt. "An Improved Microbiological Assay for Chlortetracycline in Avian Plasma." Avian Diseases 30, no. 3 (1986): 574. http://dx.doi.org/10.2307/1590424.

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42

Cardoso, Simone Gonçalves, and Elfrides E. S. Schapoval. "Microbiological assay for terbinafine hydrochloride in tablets and creams." International Journal of Pharmaceutics 203, no. 1-2 (2000): 109–13. http://dx.doi.org/10.1016/s0378-5173(00)00439-7.

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43

Cazedey, Edith Cristina Laignier, and Hérida Regina Nunes Salgado. "A novel and rapid microbiological assay for ciprofloxacin hydrochloride." Journal of Pharmaceutical Analysis 3, no. 5 (2013): 382–86. http://dx.doi.org/10.1016/j.jpha.2013.03.007.

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44

Sun, Li, Wenqing Chen, Zixin Deng, and Jian-Jiang-Zhong. "Microbiological assay for the quantitative determination of polyoxin B." Journal of Biotechnology 136 (October 2008): S476. http://dx.doi.org/10.1016/j.jbiotec.2008.07.1109.

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45

Hsu, Mei-Chich, and Yann-Jen Fann. "Determination of Dicloxacillin Preparations by Liquid Chromatography." Journal of AOAC INTERNATIONAL 75, no. 1 (1992): 26–29. http://dx.doi.org/10.1093/jaoac/75.1.26.

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Abstract A reversed-phase liquid chromatographic method was developed for the assay of dicloxacillin In bulk drugs and pharmaceutical preparations. The samples were analyzed on a μBondapak (C18) column with a mobile phase of methanol-4% acetic acid (60 + 40) at a flow rate of 1.5 mL/mln, with UV absorbance detection at 254 nm. An equation was derived showing a linear relationship between peak area ratios of dicloxacillin to dimethylphthalate (internal standard) and the dicloxacillin concentration over a range of 2-30 μg (r = 0.9999). Standard addition recoveries ranged from 98.65 to 100.74% (m

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46

Haggett, T. O. Richard, Alan R. Matheson, and Michelle Harnett. "Partial Automation of Microbiological Assays for Vitamins." Journal of AOAC INTERNATIONAL 76, no. 6 (1993): 1280–88. http://dx.doi.org/10.1093/jaoac/76.6.1280.

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Abstract The dispensing and turbidity measurement steps used in microbiological assays of B group vitamins were automated by the use of a Gilson hardware package, a specially constructed turbidimeter, an IBM PC-AT or compatible computer, and software written in-house to facilitate data entry, control the hardware, and calculate the results. Considerable improvement in the speed and precision of the turbidity measurement was achieved (1% compared to 3-12%). The precision of the dispensing operation was better than that of manual dispensing (0.02-0.8% compared to 0.4-2%), although the speed was

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47

Lourenço, Felipe Rebello, and Terezinha de Jesus Andreoli Pinto. "Antibiotic microbial assay using kinetic-reading microplate system." Brazilian Journal of Pharmaceutical Sciences 47, no. 3 (2011): 573–84. http://dx.doi.org/10.1590/s1984-82502011000300015.

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The aim of this study was to determine the optimal experimental conditions to develop a methodology for microbiological assay of apramycin employing microplate and kinetic reading mode, and to validate the developed method, through evaluation of parameters of selectivity, linearity, linear range, limits of detection and quantification, accuracy and precision. The turbidimetric assay principle is simple: the test solution is added to a suspension of test microorganism in culture media, the mixture is incubated under appropriate conditions and the microbial growth is measured by photometric read

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48

Sarma, Jayasri Das, Chandralekha Duttagupta, Esahak Ali, and Tarun K. Dhar. "Improved Microbiological Assay for Folic Acid Based on Microtiter Plating with Streptococcus faecalis." Journal of AOAC INTERNATIONAL 78, no. 5 (1995): 1173–77. http://dx.doi.org/10.1093/jaoac/78.5.1173.

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Abstract We have developed an improved method, using 96-well microtiter plates, for the microbiological assay of folic acid. With this method, the tedium of conventional microbiological analysis is substantially decreased. Culture volumes have been reduced 33-fold, and pipetting procedures have been simplified. Assay time has been reduced to 14 h, and sensitivity has increased 10-fold (0.1 ng/mL). Analytical recoveries range from 98 to 104%. Intra-assay and interassay variabilities are less than 11%. The assay does not require extensive manipulation of inoculum. Day-to-day variability has been

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Grabow, W. O. K., R. Kfir, and J. L. Slabbert. "Microbiological Methods for Safety Testing of Drinking Water Directly Reclaimed from Wastewater." Water Science and Technology 24, no. 2 (1991): 1–4. http://dx.doi.org/10.2166/wst.1991.0019.

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The following results were obtained in research on microbiological methods for assessment of the safety of drinking water directly reclaimed from wastewater: Studies on a wide variety of pathogenic micro-organisms revealed that the safety of the water could reliably be monitored by practical indicators such as standard plate counts, coliform bacteria, coliphages and acid-fast bacteria. A convenient plate incorporation modification of the Ames Salmonella mutagenicity assay proved suitable for routine quality surveillance of potentially mutagenic and carcinogenic compounds; results were confirme

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Mahmoudi, Abdelghani. "VALIDATED MICROBIOLOGICAL ASSAY FOR JOSAMYCIN DETERMINATION IN ITS PHARMACEUTICAL FORMULATIONS." Journal of Microbiology, Biotechnology and Food Sciences 10, no. 1 (2020): 33–37. http://dx.doi.org/10.15414/jmbfs.2020.10.1.33-37.

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