Relevant bibliographies by topics / Microbiological culture

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  2. Dissertations / Theses
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  5. Conference papers

Academic literature on the topic 'Microbiological culture'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 February 2022

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Journal articles on the topic "Microbiological culture"

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Repanos, C., P. Mukherjee, and Y. Alwahab. "Role of microbiological studies in management of peritonsillar abscess." Journal of Laryngology & Otology 123, no. 8 (August 2009): 877–79. http://dx.doi.org/10.1017/s0022215108004106.

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AbstractIntroduction:Peritonsillar abscess (quinsy) is one of the most common ENT emergencies. A 2002 UK audit of quinsy management revealed that an average ENT department treated 29 cases annually; the most common treatment was needle aspiration with intravenous antibiotics, and culture of the aspirate was often performed routinely. The aims of our study were to evaluate the value of routine culture of quinsy aspirates, and to establish whether the information thus gained was clinically useful.Methods:We examined the notes of patients admitted with quinsy to two hospitals in south-west England, from January 1998 to January 2004 in one hospital and from January 1995 to January 2005 in the other. A total of 577 cases was found. Aspirated pus had been sent for culture in 119 (21 per cent). These cases were examined in more detail.Results:Of the 119 patients, 78.2 per cent (93/119) were treated with either a cephalosporin or penicillin, plus metronidazole. Streptococcal species were cultured in 43.7 per cent (52/119) and anaerobes in 23.5 per cent (28/119; of these cultures, 5.9 per cent (7/119) were pure anaerobes only). All the anaerobes were sensitive to metronidazole. One of the 119 cultures, growing aerobic bacteria, was resistant to penicillin; however, this patient improved clinically on a combination of penicillin and metronidazole. No patients had their treatment changed because of culture results.Conclusions:There appears to be no need to routinely culture quinsy aspirates, based upon our findings (of 16 hospital years) and previous studies (which found no recorded episodes of treatment change as a result of culture sensitivities). The combination of penicillin or a cephalosporin, plus metronidazole appeared to be theoretically effective in 99.2 per cent (118/119) of our specimens; this finding is supported by other studies. However, the rare but potentially life-threatening complications of quinsy must be recognised.
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Aguirre, Mauricio, Eduardo Antunes Bortoluzzi, José Carlos Rivas Gutiérrez, Ronaldo Souza Ferreira Silva, Karina Maria Salvatore Freitas, Antonio Carlos Pizzolito, and Fábio Luiz Camargo Villela Berbert. "Microbiological analysis of root canal space prepared for prosthetic intracanal posts." Journal of Multidisciplinary Dentistry 10, no. 2 (August 2, 2021): 56–61. http://dx.doi.org/10.46875/jmd.v10i2.228.

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The aim of the study was to microbiologically analyze the root canal space prepared for prosthetic intracanal posts. Thus, a 2% chlorhexidine solution was used after the intraradicular preparation of ten teeth with endodontic treatment performed for prosthetic purposes and pulp vitality history. Two collections were performed for microbiological analysis: one before the use of the studied solution, showing positive microbiological culture in all cases; and another, after application for 3 minutes of 2% chlorhexidine solution. The results showed the effectiveness of the solution in nine of ten cases, presenting negative results in microbial culture.
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Desmeth, Philippe, and Ipek Kurtboke. "World Federation for Culture Collections: professionals underpinning microbial systematics." Microbiology Australia 32, no. 2 (2011): 105. http://dx.doi.org/10.1071/ma11105.

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The World Federation for Culture Collections (WFCC) is a multidisciplinary commission of the International Union of Biological Sciences (IUBS) and a Federation within the International Union of Microbiological Societies (IUMS). The WFCC is concerned with the collection, authentication, maintenance and distribution of cultures of microorganisms and cultured cells. Its aim is to promote and support the establishment of culture collections and related services, to provide liaison and networking between the collections and their users, to organise workshops and conferences, publications and newsletters and work to ensure the long-term perpetuation of important collections.
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Section, Jarren, Steven D. Gibbons, Theresa Barton, David E. Greenberg, Chan-Hee Jo, and Lawson A. B. Copley. "Microbiological Culture Methods for Pediatric Musculoskeletal Infection." Journal of Bone and Joint Surgery 97, no. 6 (March 2015): 441–49. http://dx.doi.org/10.2106/jbjs.n.00477.

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James, L. A., T. Ibrahim, and C. N. Esler. "Microbiological culture results for the femoral head." Journal of Bone and Joint Surgery. British volume 86-B, no. 6 (August 2004): 797–800. http://dx.doi.org/10.1302/0301-620x.86b6.14783.

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Chalón, Miriam C., Victoria Terán, Mario E. Arena, Rubén Oliszewki, and Silvia N. González. "Microbiological culture broth designed from food waste." Journal of Environmental Management 115 (January 2013): 1–4. http://dx.doi.org/10.1016/j.jenvman.2012.10.005.

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Lee, Chong-Suh, Kyung-Chung Kang, Sung-Soo Chung, Ki-Tack Kim, and Seong-Kee Shin. "Incidence of microbiological contamination of local bone autograft used in posterior lumbar interbody fusion and its association with postoperative spinal infection." Journal of Neurosurgery: Spine 24, no. 1 (January 2016): 20–24. http://dx.doi.org/10.3171/2015.3.spine14578.

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OBJECT The aim of this study was to examine the results of microbiological cultures from local bone autografts used in posterior lumbar interbody fusion (PLIF) and to identify their association with postoperative spinal infection. METHODS The authors retrospectively evaluated cases involving 328 patients who had no previous spinal surgeries and underwent PLIF for degenerative diseases with a minimum 1-year follow-up. Local bone was obtained during laminectomy, and microbiological culture was performed immediately prior to bone grafting. The associations between culture results from local bone autografts and postoperative spinal infections were evaluated. RESULTS The contamination rate of local bone was 4.3% (14 of 328 cases). Coagulase-negative Staphylococcus (29%) was the most common contaminant isolated, followed by Streptococcus species and methicillin-sensitive Staphylococcus aureus. Of 14 patients with positive culture results, 5 (35.7%) had postoperative spinal infections and were treated with intravenous antibiotics for a minimum of 4 weeks. One of these 5 patients also underwent reoperation for debridement during this 4-week period. Regardless of the microbiological culture results, the infection rate after PLIF with local bone autograft was 2.4% (8 of 328 cases), with 5 (62.5%) of 8 patients showing positive results on autograft culture. CONCLUSIONS The incidence of contamination of local bone autograft during PLIF was considerable, and positive culture results were significantly associated with postoperative spinal infection. Special attention focused on the preparation of local bone for autograft and its microbiological culture will be helpful for the control of postoperative spinal infection.
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Soares, Janir Alves, and Donaldo Rosa Pires Júnior. "Influence of sodium hypochlorite-based irrigants on the susceptibility of intracanal microbiota to biomechanical preparation." Brazilian Dental Journal 17, no. 4 (2006): 310–16. http://dx.doi.org/10.1590/s0103-64402006000400009.

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This study evaluated the microbiological conditions of root canals, using smears and culture from anterior teeth and premolars with necrotic pulps associated with chronic periapical pathologies, before and after biomechanical preparation (BMP). During double-flared instrumentation, 1, 2.5 and 5% sodium hypochlorite (NaOCl)-based irrigants were used in 3 groups: GI (n=39), GII (n=36) and GIII (n=36), respectively. Before BMP, all cultures were positive and the smears showed microbiologically diverse morphotypes, including fusiforms, pleomorphic, rods, cocci and filaments. Quantitetively, 20, 20 and 23 morphotypes were identified in GI, GII and GIII, respectively). After BMP, the percentages of negative cultures in GI, GII and GIII were 74.2%, 86.3% and 93.4% (p>0.05) and the number of morphotypes decreased to 14, 15 and 5, respectively. All teeth with 2 root canals and/or associated fistulas were microbiologically negative after BMP, regardless of irrigant concentration. Gram-negative morphotypes were more susceptible to the action of irrigants. After irrigation with 5% NaOCl, only structural arrangements consisting of Gram-positive cocci and bacilli persisted. Thus, BMP plus 5% NaOCl offered the best antiseptic potential because in the few positive cultures a significant reduction in the number of microbiological morphotypes was also shown (p<0.05).
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Tomanovic, Branka, and Veljko Mirovic. "Frequency and colonization rate of intravascular catheters." Vojnosanitetski pregled 61, no. 3 (2004): 255–58. http://dx.doi.org/10.2298/vsp0403255t.

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Clinical signs are not sufficiently reliable for establishing diagnosis of intravascular catheter-related infection. Therefore, microbiological confirmation, based on the culture of the catheter tip after its removal, is necessary in diagnosing the infection. The aim of this study was to determine the frequency and the degree of microbial colonization of intravascular catheters (IVK), and the risk for the onset of sepsis, by using qualitative, semiquantitative (roll plate) and quantitative (vortexing) catheter culture techniques. During the period April 2001 December 2002, 289 intravascular catheters were cultured. A total of 284 microorganisms were isolated from 217 (75%) culture-positive catheters. The frequency of isolation of some organisms was the following coagulase-negative staphylococci (CNS) 41%, Staphylococcus aureus 19% Enterococci spp 6%, other Gram-positive microorganisms 9%, Gram-negative microorganisms 21%, and fungi 4%. In 35 catheters, cultures were polymicrobial; two microorganisms were found in 25 cultures and three were found to be in 10 cultures. There were 122 (46%) intravascular catheters which were found significantly colonized. A high rate of positivity and a high rate of S. aureus isolates and Gram-negative bacteria indicate the need of establishing the exact microbiological diagnosis of these infections, and the rigorous undertaking of adequate control and preventive measures.
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Zelus, Casey, Michael Blaha, Jasmine R. Marcelin, Jasmine R. Marcelin, Kelly Cawcutt, Kelly Cawcutt, Andre C. Kalil, and Kaeli Samson. "2204. Microbiology of Pneumonia Due to Co-Infection in the ICU: Impact of Host Immune Status." Open Forum Infectious Diseases 6, Supplement_2 (October 2019): S751—S752. http://dx.doi.org/10.1093/ofid/ofz360.1884.

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Abstract Background Pneumonia epidemiology is increasingly showing the presence of co-infection due to the utilization of emerging diagnostic testing modalities such as multiplex polymerase chain reaction (PCR) panels. However, the prevalence and clinical significance of co-infection with respect to host immune status remain unclear. Methods A single-center retrospective analysis of mechanically ventilated adult patients treated in critical care units from January to October 2018 was performed on those with positive microbiological analysis of a bronchoalveolar lavage (BAL) sample. Host immune status and microbiological analyses were obtained including PCR and culture testing. Categorical variables and co-infection or immunocompetent status were assessed using Chi-Square, Fisher exact tests, or t-tests. REDCap was utilized for data abstraction and SAS software version 9.4 was used to perform all analysis. Results Of the 139 BAL samples that met inclusion criteria, 107 and 32 were obtained from immunocompetent and immunocompromised hosts, respectively. There was no statistical difference found between the frequency of co-infection detected by BAL culture with respect to host immune status. Immunocompetent patients had a higher proportion of positive bacterial cultures compared with immunocompromised (76.7% vs. 43.8% respectively, P = 0.0004). There was no significant difference seen with frequency of fungal or acid fast bacilli cultures between the two groups. Analysis of the microbiologic data obtained (figures) revealed different pathogens according to host immune status. Conclusion Pneumonia due to co-infection in critically ill, mechanically ventilated immunocompromised hosts occurs at a similar frequency regardless of host immune status, however different microbiological patterns emerge. Interestingly, patients who were not immunocompromised had a higher proportion of positive bacterial cultures compared with those who were immunocompromised. Comparative analysis of the other pathogen types may also reveal differences in detection rates if sample size is increased. Clinically, this may help guide efficient use of microbiological testing among patients based on immune status. Disclosures All authors: No reported disclosures.
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Dissertations / Theses on the topic "Microbiological culture"

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Wang, Chia-Lin. "Probiotic lactobacilli as a soy yogurt starter culture - microbiological, chemical and sensory analyses /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p1422974.

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Clarke, Kim Gail. "A reassessment of the production of acetone and butanol by Clostridium acetobutylicum in continuous culture." Doctoral thesis, University of Cape Town, 1987. http://hdl.handle.net/11427/21918.

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Bibliography: pages 154-195.
The production of acetone and butanol by Clostridium acetobutylicum P 262 was studied in continuous culture under conditions where the nutrients were present in excess of the requirements and the cell growth was limited by the products formed during the fermentation. This system differs from most continuous culture systems used to obtain solvent production where the limitation of a specific nutrient was utilised to limit the cell growth.
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Lankinen, K. S. (Kari S. ). "Catching the pneumococcus:studies focusing on carriage, epidemiology and microbiological methods." Doctoral thesis, University of Oulu, 2003. http://urn.fi/urn:isbn:9514270630.

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Abstract The purpose of this study was to develop sensitive and specific laboratory diagnostic methods for the demonstration of pneumococcal surface antigens or pneumococcus-specific antibodies in clinical samples. The work took account of epidemiological aspects of both pneumococcal disease and nasopharyngeal carriage of pneumococcus. We first compared the sensitivity of pneumococcal culture and antigen detection methods in nasopharyngeal samples in a developing country setting and then investigated the possibility of improving the sensitivity of the antigen detection by introducing an enrichment step in the procedure. — Further investigations were designed to determine the validity of pneumolysin-specific immune complex bound antibody assay as a tool for diagnosing pneumococcal ALRI in a developing country setting. Finally, we developed an enzyme immunoassay for the detection of pneumococcal capsular polysaccharide antigens, using type-specific antibodies produced in-house in rabbits through immunisation with an in-house-produced pneumococcal whole cell vaccine. The method was tested in nasopharyngeal and middle ear fluid samples. The first results indicated that antigen detection might be more sensitive than culture in demonstrating pneumococci in URT, particularly in children with prior antimicrobial therapy. Antigen detection is a feasible method for studies on pneumococci in developing countries. For type-specific demonstration of S. pneumoniae, detection of pneumococcal antigen after an enrichment step proved a sensitive method that can be applied for epidemiologic study purposes, e.g., in vaccine trials, in areas without ready access to a good microbiology laboratory. Determination of IC-bound pneumolysin IgG antibodies appears to be a useful method for species-specific diagnosis of pneumococcal infections. The results indicating pneumococcal aetiology in ALRI patients in this study compare well with the best results obtained by the use of lung aspirates. Increasing the number of serial samples improves the sensitivity of the assay, but even two samples provide more positive findings than other methods currently in routine use. Criteria of positivity need to be confirmed in subsequent larger studies with both healthy controls and patients with confirmed pneumococcal disease. It is also important to control the findings in patients with pneumonia of non-pneumococcal origin. The novel enzyme immunoassay was shown to work well with enrichment culture samples, with an almost 100% sensitivity compared with the culture. Middle ear fluid samples were too diluted for the enzyme immunoassay method used, and only 74% sensitivity compared with culture was achieved. Provided that adequate samples can be obtained, the method will be a useful complement to the current laboratory methods used to diagnose pneumococcal disease. With the existence of a broad spectrum of microbiological and immunological methods, it is imperative to seek international consensus for standard methods to demonstrate pneumococcus. Otherwise it is very difficult to compare results from different clinical studies. A WHO Working Group recently proposed a standard method for detecting upper respiratory carriage of pneumococcus, but a lot of work remains to be done in other areas of research on pneumococcal infections.
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Chapman, Katherine Emma. "Assessment of the effect of dosing regime and cell culture model on micronucleus induction in in vitro genotoxicity test systems." Thesis, Swansea University, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.678362.

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Hidalgo, Albornoz Claudio Esteban. "Microbiological analysis and control of the fruit vinegar production process." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/96296.

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En esta tesis se realizó la identificación y caracterización de los microorganismos (levaduras y bacterias acéticas) asociados a la producción de vinagres de diferentes frutas con el fin de seleccionar posibles cultivos starters para tener un mejor control del proceso. Se elaboraron vinagres a partir de uva, caqui, fresa y arándano por el método superficial principalmente, aunque, también, se utilizaron diferentes métodos sumergidos. Tras los estudios ecológicos, se realizaron pruebas de inoculación tanto para levaduras como para bacterias acéticas. En general, la inoculación resultó en una clara reducción en el tiempo de producción siendo algunas de las cepas seleccionadas buenas candidatas para utilizarse a nivel industrial. Por otra parte, a nivel tecnológico, se evaluaron diferentes diseños de barricas para mejorar la producción de vinagre por el método superficial. El aumento de la superficie de contacto con el aire es el principal parámetro a modificar para reducir el tiempo de acetificación.
In this thesis, the identification and characterization of microorganisms (yeasts and acetic acid bacteria) associated with the production of different fruit vinegars were carried out in order to select potential starter cultures to have better control of the process. Vinegars from grapes, persimmon, strawberry and highbush blueberry were mainly produced by surface method, but also submerged and Schützenbach methods were tested. After the ecological studies, inoculation tests with yeast and acetic acid bacteria were performed. Overall, inoculation improved the kinetics, shortening the time needed for the vinegar production. Furthermore, some of the strains tested could be good candidates to be used as starter cultures at industrial level. On the other hand, at technological level, the use of wood barrels with a higher air-contact surface facilitated the development of AAB, which is necessary to reduce the acetification time in traditional vinegar production (surface method).
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Macedo, Susana Nori de. "Efeito da mastite bovina sobre a composição e indicadores de higiene em leite de tanque." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/10/10135/tde-18082014-151806/.

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O objetivo geral deste estudo foi avaliar o efeito da mastite subclínica sobre a composição e a qualidade higiênica do leite de tanque de rebanhos leiteiros. Especificamente, objetivou-se avaliar o efeito da contagem de células somáticas (CCS) do leite cru de rebanhos leiteiros sobre as concentrações de gordura, proteína, sólidos totais e extrato seco desengordurado e sobre a contagem bacteriana total (CBT), contagem de psicrotróficos (CP) e contagem de coliformes (CC) do leite cru. Foram selecionados 230 rebanhos leiteiros localizados no Sul de Minas Gerais e Oeste de São Paulo, com base na média geométrica da CCS de cinco análises do mês anterior ao início das coletas. Estes rebanhos foram classificados de acordo com a CCS em três grupos: baixa (< 250.000 células/mL), média (> 250.000 e < 750.000 células/mL) e alta CCS (> 750.000 células/mL). Após a seleção dos rebanhos, amostras de leite de tanque foram coletadas quinzenalmente, por um período de três meses, totalizando 1380 amostras, que foram submetidas às análises de composição, CBT, CP e CC. Foi observado menor CBT e CC nos rebanhos com menor CCS, no entanto, rebanhos de média e alta CCS apresentaram maiores teores de gordura, proteína e sólidos totais. Foi observada média correlação entre CBT e CP (r = 0,6215) e entre CP e CC (r = 0,3692). Entre os indicadores de higiene e a composição do leite foram observadas correlações baixas e negativas entre CBT e gordura (r = -0,0585), CP e gordura (r = -0,0688) e CP e ST (r = - 0,0662). Os rebanhos com CCS < 250.000 células/mL apresentam maior qualidade higiênica do leite de tanque, no entanto, quanto à composição, rebanhos com maior CCS apresentaram maiores teores de gordura e proteína.
The general objective of this study was to evaluate the effect of subclinical mastitis on composition and hygienic quality in bulk tank milk of dairy herds. The specific objectives were to evaluated the effect of somatic cell count (SCC) of raw milk on contents of fat, protein, total solids and nonfat dry milk and on total bacterial count (TBC), psychrotrophic count (PC) and total coliform count (CC). A total of 230 dairy farms located in South of Minas Gerais and West of São Paulo were selected, based on SCC geometric mean obtained from five monthly analysis preceding the beginning of the study. These dairy farms were classified in three groups according to SCC: low (< 250,000 cells/mL), medium (> 250,001 and < 750,000 cells/mL) and high SCC (> 750,001 cells/mL). After herd selection, bulk milk samples were collected fortnightly during three months totalizing 1380 samples, which were subjected to analysis of composition, TBC, PC and CC. A decrease of TBC and CC was observed in herds with low SCC, however, herds with medium and high SCC had increase on - fat, crude protein and total solids contents. A medium correlation was observed among TBC and PC (r = 0.6215), and also among PC and CC (r = 0.3692). Based on hygiene indicators and the milk composition, it was observed a low and negative correlation among TBC and fat (r = -0.0585), PC and fat (r = -0.0688) and PC and total solids (r = -0.0662). Dairy herds with low SCC had higher hygienic quality of bulk tank milk, however, considering the composition, herds with higher SCC showed higher milk fat and protein concentration.
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Nassar, Alessandra Figueiredo de Castro. "Desenvolvimento de ensaio imunoenzimático (ELISA) indireto na detecção de anticorpos anti-Corynebacterium pseudotuberculosis em ovinos (Ovis aries, Linnaeus, 1758)." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10136/tde-29082012-140320/.

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A linfadenite caseosa é uma doença infectocontagiosas, de ocorrência mundial, que acomete caprinos e ovinos, caracterizada pela formação de abscessos em gânglios linfáticos superficiais, e alguns casos órgãos e linfonodos internos. É uma enfermidade causada pelo Corynebacterium pseudotuberculosis, responsável por grandes perdas econômicas na caprinocultura e ovinocultura. O presente trabalho teve por objetivo desenvolver um teste sorológico sensível e específico para detectar anticorpos anti-C. pseudotuberculosis em ovinos. Os animais foram classificados em dois grupos, com sintomatologia aparente para linfadenite caseosa (n=103) colhidos a campo, onde foram coletados amostras de soro e punção de linfonodos aumentados; e animais sem sintomatologia aparente (n=50) dos quais as amostras de soro e fragmentos de pulmão e linfonodo mediastínico foram colhidos em frigorífico. Em ambas as amostras a confirmação da presença e ausência da doença foi realizada através das provas de cultivo microbiológico e PCR, considerados nesse estudo como padrão para classificação dos animais. O resultado do cultivo microbiológico dos animais com sintomatologia aparente foi 53,5% (55/103) identificadas como C. pseudotuberculosis através de provas bioquímicas, e com a utilização da PCR, 46,5% (48/103) das amostras foram positivas para C. pseudotuberculosis. Com relação aos animais sem sintomatologia aparente, todas as amostras foram negativas no cultivo microbiológico e PCR (0/50). Na padronização do ELISA indireto foram utilizados 42 soros positivos e 43 soros negativos confirmados no cultivo microbiológico e PCR para linfadenite caseosa. A média de absorbância foi 1,88 com desvio padrão de 0,43, para as amostras positivas e, para as amostras negativas a média de 0,71 e desvio padrão 0,18. Dessa forma foram consideradas positivas, as amostras que apresentaram na reação de ELISA valor da DO> 1,1 e negativas de <1,1. A análise gráfica empregada no presente trabalho, curva ROC, permitiu encontrar o valor do ponto de corte associado à combinação dos parâmetros de sensibilidade e especificidade, assim, a acurácia do teste pôde descriminar indivíduos doentes de não doentes. A utilização de técnicas sorológicas no diagnóstico da linfadenite caseosa permitirá o controle epidemiológico da doença, porém não substitui o cultivo microbiológico, técnica considerada padrão ouro, e pode ser utilizada como teste de triagem ou mesmo na comercialização de animais, visto que a doença muitas vezes é de caráter inaparente, o que inviabiliza o diagnóstico clínico e microbiológico.
Caseous lymphadenitis is an infectious disease of worldwide occurrence that affects sheep and goats, characterized by the formation of abscesses in superficial lymph nodes, and sometimes internal organs and lymph nodes. It is caused by Corynebacterium pseudotuberculosis, responsible for great economic losses in goat and sheepproduction. This study aimed to develop a sensitive and specific serological test to detect anti- C. pseudotuberculosis in sheep. The animals were divided into two groups, the first one encompassing the ones with apparent caseous lymphadenitis symptoms (n = 103), where serum samples and puncture of enlarged lymph nodes were collected, and the second one with animals with no apparent symptoms (n = 50) of which samples (lung fragments, serum and and mediastinal lymph nodes) were inspected and harvested in the slaughterhouse. In both groups the presence and absence of the disease was carried through the evidence of microbiological culture and PCR, considered as the standard for the groups classification. Microbiological culture results of animals with apparent symptoms was 53.5% (55/103) identified as C. pseudotuberculosis by biochemical tests, and allied to PCR, 46.5% (48/103) of the samples were positive for C. pseudotuberculosis. Regarding animals without apparent symptoms, all samples were negative in microbiological culture and PCR (0/50). For the standardization of indirect ELISA we used 42 positive sera and 43 negative sera confirmed by microbiological culture and PCR for caseous lymphadenitis. The mean absorbance was 1.88 with standard deviation of 0.43 for the positive samples, and for negative samples the average was 0.71 and standard deviation 0.18. Thus we considered as positive, the samples with DO value > 1.1 and negative <1.1. The graphical analysis employed in this study, ROC curve, allowed us to find the cutoff value allied to the association of sensitivity and specificity parameters, thus the test accuracy was able to discriminate sick from not sick individuals. The use of serological techniques for the diagnosis of caseous lymphadenitis will allow the epidemiological control of the disease, but does not replace the microbiological culture, considered as the gold standard technique, and can be used as a screening test or animals trade, since the disease condition often is unapparent, which derails the clinical diagnosis and microbiological.
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Faour-Klingbeil, Dima. "The microbiological safety of fresh produce in Lebanon : a holistic 'farm-to-fork chain' approach to evaluate food safety, compliance levels and underlying risk factors." Thesis, University of Plymouth, 2017. http://hdl.handle.net/10026.1/8654.

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The consumption of unsafe fresh vegetables has been linked to an increasing number of outbreaks of human infections. In Lebanon, although raw vegetables are major constituents of the national cuisine, studies on the safety of fresh produce are scant. This research employed a holistic approach to identify the different stages of the food chain that contribute to the microbiological risks on fresh produce and the spreading of hazards. A thorough analysis of the institutional and regulatory framework and the socio-political environment showed that the safety of local fresh produce in Lebanon is at risk due to largely unregulated practices and shortfalls in supporting the agricultural environment as influenced by the lack of a political commitment. Microbiological analysis showed that the faecal indicator levels ranged from < 0.7 to 7 log CFU/g (Escherichia coli), 1.69-8.16 log CFU/g (total coliforms) and followed a significantly increasing trend from fields to the post-harvest washing area. At washing areas, Salmonella was detected on lettuce (6.7% of raw vegetables from post-harvest washing areas). This suggested that post-harvest cross-contamination occurs predominantly in the washing stage. At retails, a combination of observation and self-reported data provided an effective tool in assessing knowledge, attitudes and practices. It showed that the food safety knowledge and sanitation practices of food handlers were inadequate, even among the better trained in corporate-managed SMEs. Overall, the microbiological quality of fresh-cut salad vegetables in SMEs was unsatisfactory. The link between Staphylococcus aureus and microorganism levels on fresh salads vegetables and the overall inspection scores could not be established. On the other hand, inspection ratings on individual components, e.g., cleanliness and cross-contamination preventive measures showed significant correlation with Listeria spp. levels. Together, results confirmed that inspection ratings don’t necessary reflect the microbiological safety of fresh vegetables and that the application of control points of risk factors that likely to contribute to microbial contamination in the production environment are essential. The washing methods were limited in their effectiveness to reduce the contamination of parsley with Salmonella. In general, the pre-wash chopping and storing of parsley at 30ºC reduced the decontamination effect of all solutions, including sodium dichloroisocyanurate which was reduced by 1.3 log CFU/g on both intact and chopped leaves stored at 30ºC. In such conditions, the transfer rate of Salmonella from one contaminated parsley to subsequently chopped clean batches on the same cutting board(CB) recorded 60%-64%. Furthermore, the transmission of Salmonella persisted via washed CBs stored at 30°C for 24 h. It is recommended to keep parsley leaves unchopped and stored at 5ºC until wash for an optimum decontamination effect and to apply vigilant sanitation of CBs after use with fresh produce. This research presented important data for quantitative risk assessment for Salmonella in parsley and useful descriptive information to inform decision-makers and educators on microbial hazards associated with fresh produce in Lebanon. It also highlighted the risks areas that require urgent interventions to improve food safety. Considering the complex institutional and political challenges in Lebanon, there is an obvious need to direct development programs and support towards local agriculture production, effective education strategies and growing awareness of consumers and stakeholders on food safety related risks.
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Ottati-de-Lima, Emma Luize [UNESP]. "Produção de Metarhizium anisopliae (METSCH.) SOROK. e Beauveria bassiana (BALS.) VUILL. em diferentes substratos e efeito da radiação ultravioleta e da temperatura sobre estruturas infectivas desses entomopatógenos." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/105406.

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Os objetivos deste trabalho foram avaliar diferentes meios de cultura na produção, semi-sólida e líquida, de propágulos de fungos de M. anisopliae e B. bassiana, bem como a tolerância desses propágulos a ação da radiação ultravioleta e da temperatura. Os experimentos foram conduzidos no Laboratório de Controle Biológico do Instituto Biológico, em Campinas, SP. Foram realizados, para M. anisopliae e B. bassiana, 6 repetições para cada um dos 17 tratamentos: amido de milho, arroz integral, arroz parboilizado, arroz tipo 1 (testemunha), arroz tipo 2, aveia em flocos, canjiquinha, farelo de trigo, farinha de mandioca crua, farinha de milho amarela, farinha de trigo especial, fubá, milho em grãos, polvilho azedo, soja em grãos, trigo moído e turfa. A viabilidade foi feita em placas de Petri plásticas contendo BDA. Para os ensaios com radiação ultravioleta e temperatura, utilizou-se a mesma metodologia para a viabilidade, mas cada tratamento sendo exposto à radiação por 0, 25 e 50 segundos e, em diferentes temperaturas: 20, 25, 30 e 35oC. Inoculou-se, em torre de Potter, 2 mL da suspensão de fungo de cada tratamento em lagartas de Diatraea saccharalis. Para a concentração os melhores tratamentos de M. anisopliae e B. bassiana foram: arroz parboilizado tipo 1, arroz tipo 1, arroz tipo 2, farinha de milho amarela, fubá e trigo moído. A viabilidade de todos os tratamentos foi superior a 94,00%; quanto maior o tempo de exposição ao ultravioleta menor foi o número de conídios férteis. À temperatura de 35oC ocorreu perda significativa da viabilidade de conídios e todos os tratamentos se mostraram virulentos. Para a 2 produção líquida de blastosporos de M. anisopliae e B. bassiana, foram avaliados 12 tratamentos, com 6 repetições cada, compostos pelas combinações entre as concentrações de Carbono (C), na forma...
The goals of this work were to evaluate different medias (semi-solid and liquid) as for the production of M. anisopliae and B. Bassiana propagules, and also the tolerance of these propagules to ultraviolet radiation and temperature. The experiments were carried out at the Biological Control Laboratory of the Instituto Biológico, at Campinas, São Paulo, Brazil. For both M. anisopliae and B. bassiana, 6 repetitions were performed for each one of the 17 treatments: corn stearch, full rice, parboiled rice, type 1 rice, type 2 rice, oat flakes, canjiquinha, wheat flour, raw cassava flour, yellow corn flour, special wheat flour, corn flour, corn in grains, cassava stearch, soy in grains, crushed wheat and turf. The viability analysis was done in plastic plates containing BDA. For each one of the bioassays in ultraviolet and temperature exposition, the same methodology was used for viability analysis, but each treatment was exposed to the UV radiation during 0, 25 and 50 seconds, and at different temperatures: 20, 25, 30 and 35oC. Using the Potter tower, 2 mL of fungus suspension, from each tratment, were inoculated into the Diatraea saccharalis caterpillars. Regarding the sporulation, the best M. anisopliae and B. bassiana treatments were: parboiled rice, type 1 rice, type 2 rice, yellow corn flour, corn flour and crushed wheat. The viability of all the treatments was superior to 94,00%. Also, as longer was the duration of the exposition to the UV, as smaller was the number of fertile conidia. At 35oC, a significant loss of conidia viability was observed, and all the treatments have shown some level of virulence. For the M. anisopliae and B. bassiana blastospores production over liquid media, 12 treatments were 4 evaluated, with 6 repetitions each, composed by the combinations of carbon (C), presented as D-glicose anidra (40% Carbon) and... (Complete abstract click electronic access below)
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Cornut, Pierre-Loïc. "Apport des techniques de biologie moléculaire dans le diagnostic microbiologique des endophtalmies exogènes aigues." Thesis, Lyon 1, 2013. http://www.theses.fr/2013LYO10092.

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La documentation microbiologique systématique des infections endoculaires (ou endophtalmies) permet la confirmation du caractère infectieux de l'atteinte et l'adaptation éventuelle du traitement à l'échelle individuelle, ainsi que la caractérisation épidémiologique du spectre bactérien à l'échelle collective. Longtemps réservée au domaine de la recherche, l'utilisation des techniques de biologie moléculaire en complément des techniques de culture conventionnelle a pris une place importante dans la démarche diagnostique de ces affections au cours des dernières années. Ces techniques de diagnostic microbiologique sont particulièrement utiles pour la recherche de bactéries difficiles voire impossibles à cultiver du fait de leurs propriétés intrinsèques, de leur présence en très faible quantité, de leur séquestration sur le matériel prothétique ou de leur inactivation par une antibiothérapie préalable. Elles sont basées sur le principe commun de la Polymerase Chain Reaction (PCR) qui permet après une étape d'extraction, l'amplification puis la détection d'Acide Désoxyribo Nucléique (ADN) bactérien initialement présent en quantité infime au sein d'un prélèvement endoculaire. Ces PCR effectuées en mode conventionnel ou en temps réel permettent soit la détection d'ADN bactérien sans a priori (PCR pan bactérienne suivie en général de séquençage) soit la mise en évidence d'un fragment d'ADN spécifique de genre ou d'espèce bactérien (PCR spécifiques). En pratique, afin d'optimiser la détection des micro-organismes responsables d'endophtalmies, il est préférable dans la plupart des cas d'obtenir un prélèvement précoce de vitré et d'appliquer sur ce prélèvement une culture conventionnelle et une technique de biologie moléculaire par PCR pan bactérienne, les deux approches étant complémentaires. Pour les prélèvements effectués lors de la vitrectomie postérieure, l'analyse du vitré dilué par PCR pan bactérienne est suffisante. Les techniques de biologie moléculaire sont plus sensibles que les cultures pour l'identification de bactéries à croissance lente ou difficile (Granulicatella, Moraxella, P. acnes) et offrent une possibilité alternative de diagnostic étiologique lorsque la culture conventionnelle est mise en défaut, en particulier lorsque le patient a déjà bénéficié d'une antibiothérapie intra vitréenne. Les techniques de PCR spécifiques ciblant les genres les plus fréquemment impliqués dans les endophtalmies (Staphylococcus et Streptococcus) sont utiles en seconde intention en cas de négativité des analyses en culture et PCR pan bactérienne, en particulier lorsque seul un prélèvement d'humeur aqueuse est disponible
The systematic microbiological documentation of endophthalmitis allows the confirmation of the infectious nature of the disease and the possible adaptation of treatment at the individual level and, at the collective level, the epidemiological characterization of the bacterial spectrum of endophthalmitis. Long reserved for research, the use of molecular biology techniques to complement conventional culture techniques has become important for the diagnosis of endophthalmitis in recent years. These new diagnostic techniques are particularly useful for the microbiological study of bacteria that are difficult or impossible to grow, due to their intrinsic properties, their presence in very small inoculum, their sequestration on prosthetic materials, or their inactivation by prior antibiotic treatment. These techniques are based on the polymerase chain reaction (PCR), which allows the amplification and detection of extracted bacterial deoxyribonucleic acid (DNA) that is initially present in very small quantities in an ocular sample. In practice, these conventional or real-time PCRs allow either the a priori detection of bacterial DNA (universal PCR) or the identification of a specific DNA fragment of a bacterial genus or species (specific PCR). New techniques of PCR will allow more rapid bacterial identification and also characterization of genotypic properties, such as gene of virulence or antibioresistance
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Books on the topic "Microbiological culture"

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C, Parks Lawrence, ed. Handbook of microbiological media. Boca Raton: CRC Press, 1993.

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C, Parks Lawrence, ed. Handbook of microbiological media. 2nd ed. Boca Raton: CRC Press, 1997.

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Atlas, Ronald M. Handbook of microbiological media. 4th ed. Boca Raton: Taylor & Francis, 2010.

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Zimbro, Mary Jo. Difco & BBL manual: Manual of microbiological culture media. Sparks, Md: Becton, Dickinson, 2003.

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Bridson, E. The development, manufacture and control of microbiological culture media. [Basingstoke, Hants]: Unipath Ltd., 1994.

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Atlas, Ronald M. Handbook of microbiological media for the examination of food. 2nd ed. Boca Raton: Taylor & Francis, 2006.

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Atlas, Ronald M. Handbook of microbiological media for the examination of food. Boca Raton: CRC Press, 1995.

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Microbiological applications: Laboratory manual in general microbiology. 8th ed. Boston: McGraw-Hill, 2002.

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Benson, Harold J. Microbiological applications: Laboratory manual in general microbiology. 7th ed. Boston, Mass: WCB/McGraw-Hill, 1998.

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Microbiological applications: Laboratory manual in general microbiology. 8th ed. Boston: McGraw-Hill, 2002.

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Book chapters on the topic "Microbiological culture"

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Chauhan, Abhishek, and Tanu Jindal. "Microbiological Culture Media: Types, Role and Composition." In Microbiological Methods for Environment, Food and Pharmaceutical Analysis, 23–66. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-52024-3_3.

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Subudhi, Chinari P. K. "Principles of Urine Microscopy and Microbiological Culture." In Imaging and Technology in Urology, 131–34. London: Springer London, 2012. http://dx.doi.org/10.1007/978-1-4471-2422-1_29.

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da Silva, Neusely, Marta Hiromi Taniwaki, Valéria Christina Amstalden Junqueira, Neliane Ferraz de Arruda Silveira, Margarete Midori Okazaki, and Renato Abeilar Romeiro Gomes. "Guidelines on preparation of culture media." In Microbiological Examination Methods of Food and Water, 399–410. Second edition. | Leiden, The Netherlands ; Boca Raton : CRC Press/Balkema, [2018]: CRC Press, 2018. http://dx.doi.org/10.1201/9781315165011-24.

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da Silva, Neusely, Marta Hiromi Taniwaki, Valéria Christina Amstalden Junqueira, Neliane Ferraz de Arruda Silveira, Margarete Midori Okazaki, and Renato Abeilar Romeiro Gomes. "Guidelines on preparation of culture media." In Microbiological Examination Methods of Food and Water, 399–410. Second edition. | Leiden, The Netherlands ; Boca Raton : CRC Press/Balkema, [2018]: CRC Press, 2017. http://dx.doi.org/10.1201/b13740-24.

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Kannojia, Pooja, Abhijeet S. Kashyap, Nazia Manzar, Divya Srivastava, Udai B. Singh, Sushil K. Sharma, and Pawan K. Sharma. "Preservation of Fungal Culture with Special Reference to Mineral Oil Preservation." In Microbiological Advancements for Higher Altitude Agro-Ecosystems & Sustainability, 433–45. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-1902-4_21.

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Saier, Milton H. "Application of the Microbiological Approach to the Study of Passive Monovalent Salt Transport in a Kidney Epithelial Cell Line, MDCK." In Tissue Culture of Epithelial Cells, 51–67. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4814-6_4.

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Panda, Amrita Kumari, Rojita Mishra, and Satpal Singh Bisht. "Microbiological Tools for Cultural Heritage Conservation." In Microbial Biotechnology Approaches to Monuments of Cultural Heritage, 137–49. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3401-0_8.

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Panda, Amrita Kumari, Rojita Mishra, and Satpal Singh Bisht. "Correction to: Microbiological Tools for Cultural Heritage Conservation." In Microbial Biotechnology Approaches to Monuments of Cultural Heritage, C1. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3401-0_11.

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Ulrych, Jan. "The Value of Microbiological Cultures: When to Perform Them and How to Read Them." In Infections in Surgery, 215–26. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62116-2_19.

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Sandle, Tim. "Microbiological culture media." In Pharmaceutical Microbiology, 47–61. Elsevier, 2016. http://dx.doi.org/10.1016/b978-0-08-100022-9.00005-0.

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Conference papers on the topic "Microbiological culture"

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Stojanova, Monika, Olga Najdenovska, and Dragutin Đukic. "THE INFLUENCE OF TWO STARTER CULTURES ON THE MICROBIOLOGICAL STABILITY OF MACEDONIAN TRADITIONAL SAUSAGE." In XXVI savetovanje o biotehnologiji sa međunarodnim učešćem. University of Kragujevac, Faculty of Agronomy, 2021. http://dx.doi.org/10.46793/sbt26.441s.

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The aim of this paper is to monitor the influence of two starter cultures on the microbiological stability of Macedonian traditional sausage. The research covered three variants: Control variant; Variant 2: with addition of starter culture CS-300; Variant 3: with addition of starter cultures CS-300 and BLC-78. The total bacteria count and Lactobacillus sp. in all three variants decreases compared to the initial value. There is no presence of Escherichia coli and Enterobacteriaceae.Starter culture CS-300 is recommended, while better stability of the microflora is achieved during the storage period, as well as a good quality. At the same time, the use of nitrite salt is eliminated, which results in getting a safe product.
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Aitken, Moira, Diana Bilton, Ricardo Pinero, Howard Fox, and Brett Charlton. "Mannitol Inhaler Device Culture Study: No Evidence Of An Increased Microbiological Contamination." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a1125.

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Gardner, LE, C. Hogg, SB Carr, A. Shoemark, G. Marsh, and JC Davies. "S60 Upper vs. lower airway microbiological culture in children with respiratory symptoms." In British Thoracic Society Winter Meeting 2019, QEII Centre, Broad Sanctuary, Westminster, London SW1P 3EE, 4 to 6 December 2019, Programme and Abstracts. BMJ Publishing Group Ltd and British Thoracic Society, 2019. http://dx.doi.org/10.1136/thorax-2019-btsabstracts2019.66.

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Sokolov, A. O., L. A. Dykman, A. A. Galitskaya, and O. I. Sokolov. "Detection and primary characterization of Rothia amarae in a suspension culture of Arabidopsis thaliana (Heynh.) cells." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.232.

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The presence of non-pathogenic bacterial microflora in Arabidopsis thaliana suspension culture has been shown to coexist latently with plant cells for a long time. According to the results of 16S rRNA sequencing, it was shown that the detected bacteria belong to the species Rothia amarae. Using microbiological, microscopic and immunochemical methods, the correspondence of the obtained strain to the species Rothia amarae was also confirmed.
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Gvozdev, Yu A., T. M. Zimina, L. A. Kraeva, and G. N. Hamdulaeva. "Image recognition of juvenile colonies of pathogenic microorganisms in the culture based microbiological method implemented in bioMEMS device for express species identification." In 2016 IEEE NW Russia Young Researchers in Electrical and Electronic Engineering Conference (EIConRusNW). IEEE, 2016. http://dx.doi.org/10.1109/eiconrusnw.2016.7448292.

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Aldosari, M., and Oksana Ksenofontova. "Characteristics of epiphytic microorganisms of wheat plants antagonists of phytopathogenic fungi." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.016.

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Epiphytic microbiological complex of the surface of spring wheat plants of the Saratov 70 variety was studied. Among of the selected epiphyte cultures, strains of fungicide producers were screened for the genera Alternaria, Aspergillus and Fusarium.
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Ferdes, Mariana, and Rodica Roxana Constantinescu. "Isolation and characterization of fungal and bacterial proteolytic strains from chrome shavings." In The 8th International Conference on Advanced Materials and Systems. INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania, 2020. http://dx.doi.org/10.24264/icams-2020.ii.9.

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The chrome shavings waste obtained as a result of the leather finishing process accumulates in a large volume in tanneries and represent a major problem for the environment. This waste are particularly resistant to attack of microorganisms, due to the significant concentration of chromium and are thus difficult to degrade. In this study, chrome shavings were analyzed microbiologically by determining the total number of germs and the number of yeasts and molds on specific culture media. Several bacterial and fungal strains were isolated from the cultures in Petri dishes, after the growth of the colonies. These strains were characterized in terms of the production of proteolytic enzymes, by a method of screening on the media with casein, which allows the determination of proteolytic indices of microorganisms. As a result of the tests performed, five bacterial strains probably belonging to the genus Bacillus and two fungal strains from the genera Penicillium and Cladosporium were selected.
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Bomfim, Isabela Maria Fortaleza Neves, Yanne Naara Teixeira De Carvalho, Joyce Hercília Jerônimo Lins, Amanda Viera Barros, and Renato Motta Neto. "CULTURAS DE VIGILÂNCIA DE RESISTÊNCIA DE PACIENTES INTERNOS EM UNIDADE DE TERAPIA INTENSIVA DO HOSPITAL REFERÊNCIA EM DOENÇAS INFECCIOSAS DO RIO GRANDE DO NORTE." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1202.

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Introdução: As Infecções Relacionadas à Assistência à Saúde (IRAS) são infeções adquiridas no curso de uma internação em uma unidade hospitalar. As Unidades de Terapia Intensiva (UTI) são os locais onde frequentemente ocorrem surtos por bactérias multirresistentes, visto a pressão seletiva e a disseminação de genes de resistência nestes ambientes. As culturas de vigilância de resistência são um conjunto de técnicas de isolamento e identificação de microrganismos resistentes aos antimicrobianos em indivíduos colonizados com a finalidade de prevenir as IRAS. Objetivos: Este trabalho teve como meta traçar a frequência fenotípica e genotípica de bactérias multirresistentes dos pacientes internados na UTI do hospital estadual referência em doenças infectocontagiosas. Materiais e Métodos: A coleta das amostras foi feita nos sítios axilar, nasal e retal e processadas no LABMIC/UFRN. Os swabs de cada sítio foram semeados em caldo BHI para enriquecimento, e foi procedido o isolamento primário (IP) nos ágares sangue e MacConkey. Transcorridas 24 horas do IP, determinou-se as características morfotintoriais e as provas bioquímicas para a identificação dos espécimes. Identificada a bactéria, foi procedido o teste de sensibilidade aos antimicrobianos, através da técnica de ágar disco-difusão, e testes fenotípicos para determinação do perfil de resistência das bactérias encontradas. Os espécimes com perfil de resistência aos carbapenêmicos passaram por PCR convencional para a determinação da presença dos genes blaIMP-1, blaIMP-2, blaVIM-1, blaVIM-2, blaOXA-23, blaOXA-48 e blaKPC e mcr-1. Resultados: Dezenove amostras apresentaram resistência aos carbapenêmicos e a CIM destes para o Imipenem e Polimixina B demonstrou 94,7% e 31,6% de resistência, respectivamente. Quanto aos genes testados, o gene blaNDM-1 foi expresso por 21% das amostras e o gene blaKPC por 42,1% das amostras. Os demais não foram expressos. As cepas encontradas neste estudo são responsáveis por falhas terapêuticas severas e demonstram a complexidade da problemática da resistência bacteriana. Conclusão: Este trabalho denota a necessidade de medidas de contenção dos microrganismos que causam as IRAS e visa contribuir para o aprimoramento das condutas de vigilância em UTIs, auxiliando na prevenção e controle de transmissão de bactérias multirresistentes e no estabelecimento de ações de controle de surtos nestas unidades.
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Santos, Joana Darc Silva, and Ana Karla Araújo Montenegro. "ESTREPTOCOCOS B COMO CAUSA DE INFECÇÃO EM MULHERES GRÁVIDAS: UMA REVISÃO BIBLIOGRÁFICA." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1158.

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Introdução: Os estreptococos do grupo B (EGB) ou Streptococcus agalactiae, são bactérias gram-positivas, catalase-negativas, anaeróbias facultativas, que apresentam forma esférica ou ovóide, agrupando-se em cadeia, sendo um comensal comum do trato genital feminino; estando ainda, relacionada a infecções invasivas em recém-nascidos (como sepse, meningite e pneumonia). A incidência de colonização no trato genital de gestantes, relacionada às complicações da evolução da gravidez, varia de 10% a 30% e a transmissão vertical ocorre em 30 a 70% dos recém-nascidos. Objetivos: o presente estudo objetivou analisar através da literatura específica, as infecções neonatais por EGB, a fim de apresentar as possíveis consequências da colonização e a relevância do diagnóstico para a detecção pré-parto da bactéria, inspirando estratégias para a redução significativa da incidência. Material e métodos: as informações para este estudo foram levantadas a partir de artigos em revistas científicas, publicados em português, espanhol e inglês, num recorte temporal de 2001 a 2021, nas bases de dados PubMed, SciELO e MEDLINE. Resultados: foram encontrados 1.476 artigos utilizando as palavras chave, gravidez e Streptococcus agalactiae. Entretanto, visto a inespecificidade dos artigos, utilizamos os que apresentavam os descritores do título, totalizando para abordagem neste estudo 79 artigos. Após a leitura dos resumos, 23 estudos foram avaliados por completo, obtendo um número final de 9 artigos que demonstraram a importância para o tema proposto no estudo. Embora a abrangência dos exames pré-natais tenha aumentado, observa-se que ainda hoje a cultura do EGB, não é realizada de forma rotineira durante o pré-natal, o que explica a alta prevalência de colonização materna e, consequentemente, de doenças neonatais. Conclusão: diante da escassez de informações específicas sobre a doença, fica evidente a necessidade de novos estudos no Brasil a fim de subsidiar o desenvolvimento de políticas públicas de saúde, no intuito de fortalecer a atenção dada pelos órgãos responsáveis ao rastreamento do patógeno e a profilaxia durante o acompanhamento pré-natal.
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Porcino, Andrezza Oliveira, and Marileide Oliveira Neto. "STENOTROPHOMONAS MALTOPHILIA E OS MECANISMOS DE RESISTÊNCIA: REVISÃO BIBLIOGRÁFICA." In I Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1631.

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Abstract:
Introdução: Stenotrophomonas maltophilia é um bacilo Gram negativo não fermentador e oportunista, afetando principalmente pacientes imunocomprometidos em unidades de terapia intensiva ou com doenças de bases, neoplasias e fibrose cística, é naturalmente resistente aos carbapenêmicos e apresenta maior sensibilidade a sulfametoxazol/trimetoprima. Objetivo: Avaliar o perfil de sensibilidade e descrever os mecanismos de resistência relacionada a essa bactéria. Material e Métodos: A pesquisa aconteceu através de uma revisão bibliográfica da literatura, realizada no mês de julho de 2021. O levantamento de dados foi realizado nas bases de dados da SciELO, Lilacs e PubMed no período compreendido entre 2015 e 2021. Resultados: Foram identificados inicialmente 79 artigos na literatura, destes 18 artigos foram selecionados para o trabalho. Os resultados apontaram: S. maltophilia tem a capacidade de se aderir a corpos estranhos e formar biofilme, conferindo proteção contra as defesas do hospedeiro e aos mecanismos de ação dos antimicrobianos, possui resistência intrínseca devida à redução de permeabilidade da membrana externa, bomba de efluxo de múltiplas drogas, alteração do sítio alvo, além de mecanismos específicos de resistência como enzimas modificadoras de aminoglicosídeos e produção de metalo-β-lactamase. Outro resultado evidenciado é que pacientes com hipersensibilidade ou que apresentem resistência a sulfametoxazol/trimetoprima poderão receber a combinação de drogas alternativos como ticarcilina-clavulanato, ceftazidima, levofloxacino e moxifloxacino, levando em consideração os resultados da cultura. Conclusão: O aumento da incidência desta bactéria foi evidenciado e está relacionado aos avanços nas terapias quimioterápicas, aumento no uso de equipamentos invasivos e uso indiscriminado de antibióticos de amplo espectro, principalmente os carbapenêmicos. Apesar dessa multirresistência ser uma realidade, a seleção de antibióticos para uso no tratamento da infecção apresenta-se como um desafio tanto para o laboratório quanto para o médico não apenas por causa dos problemas associados à resistência intrínseca a bactéria, mas também por causa da falta de padronização nos testes de sensibilidade. Embora não faltem estudos in vitro sobre a atividade de drogas antibacterianas contra S. maltophilia, faltam estudos clínicos para determinar o melhor tratamento para essa infecção bacteriana.
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