Journal articles: 'Microbiological culture'

1

Repanos, C., P. Mukherjee, and Y. Alwahab. "Role of microbiological studies in management of peritonsillar abscess." Journal of Laryngology & Otology 123, no. 8 (August 2009): 877–79. http://dx.doi.org/10.1017/s0022215108004106.

Full text

Abstract:

AbstractIntroduction:Peritonsillar abscess (quinsy) is one of the most common ENT emergencies. A 2002 UK audit of quinsy management revealed that an average ENT department treated 29 cases annually; the most common treatment was needle aspiration with intravenous antibiotics, and culture of the aspirate was often performed routinely. The aims of our study were to evaluate the value of routine culture of quinsy aspirates, and to establish whether the information thus gained was clinically useful.Methods:We examined the notes of patients admitted with quinsy to two hospitals in south-west England, from January 1998 to January 2004 in one hospital and from January 1995 to January 2005 in the other. A total of 577 cases was found. Aspirated pus had been sent for culture in 119 (21 per cent). These cases were examined in more detail.Results:Of the 119 patients, 78.2 per cent (93/119) were treated with either a cephalosporin or penicillin, plus metronidazole. Streptococcal species were cultured in 43.7 per cent (52/119) and anaerobes in 23.5 per cent (28/119; of these cultures, 5.9 per cent (7/119) were pure anaerobes only). All the anaerobes were sensitive to metronidazole. One of the 119 cultures, growing aerobic bacteria, was resistant to penicillin; however, this patient improved clinically on a combination of penicillin and metronidazole. No patients had their treatment changed because of culture results.Conclusions:There appears to be no need to routinely culture quinsy aspirates, based upon our findings (of 16 hospital years) and previous studies (which found no recorded episodes of treatment change as a result of culture sensitivities). The combination of penicillin or a cephalosporin, plus metronidazole appeared to be theoretically effective in 99.2 per cent (118/119) of our specimens; this finding is supported by other studies. However, the rare but potentially life-threatening complications of quinsy must be recognised.

APA, Harvard, Vancouver, ISO, and other styles

2

Aguirre, Mauricio, Eduardo Antunes Bortoluzzi, José Carlos Rivas Gutiérrez, Ronaldo Souza Ferreira Silva, Karina Maria Salvatore Freitas, Antonio Carlos Pizzolito, and Fábio Luiz Camargo Villela Berbert. "Microbiological analysis of root canal space prepared for prosthetic intracanal posts." Journal of Multidisciplinary Dentistry 10, no. 2 (August 2, 2021): 56–61. http://dx.doi.org/10.46875/jmd.v10i2.228.

Full text

Abstract:

The aim of the study was to microbiologically analyze the root canal space prepared for prosthetic intracanal posts. Thus, a 2% chlorhexidine solution was used after the intraradicular preparation of ten teeth with endodontic treatment performed for prosthetic purposes and pulp vitality history. Two collections were performed for microbiological analysis: one before the use of the studied solution, showing positive microbiological culture in all cases; and another, after application for 3 minutes of 2% chlorhexidine solution. The results showed the effectiveness of the solution in nine of ten cases, presenting negative results in microbial culture.

APA, Harvard, Vancouver, ISO, and other styles

3

Desmeth, Philippe, and Ipek Kurtboke. "World Federation for Culture Collections: professionals underpinning microbial systematics." Microbiology Australia 32, no. 2 (2011): 105. http://dx.doi.org/10.1071/ma11105.

Full text

Abstract:

The World Federation for Culture Collections (WFCC) is a multidisciplinary commission of the International Union of Biological Sciences (IUBS) and a Federation within the International Union of Microbiological Societies (IUMS). The WFCC is concerned with the collection, authentication, maintenance and distribution of cultures of microorganisms and cultured cells. Its aim is to promote and support the establishment of culture collections and related services, to provide liaison and networking between the collections and their users, to organise workshops and conferences, publications and newsletters and work to ensure the long-term perpetuation of important collections.

APA, Harvard, Vancouver, ISO, and other styles

4

Section, Jarren, Steven D. Gibbons, Theresa Barton, David E. Greenberg, Chan-Hee Jo, and Lawson A. B. Copley. "Microbiological Culture Methods for Pediatric Musculoskeletal Infection." Journal of Bone and Joint Surgery 97, no. 6 (March 2015): 441–49. http://dx.doi.org/10.2106/jbjs.n.00477.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

James, L. A., T. Ibrahim, and C. N. Esler. "Microbiological culture results for the femoral head." Journal of Bone and Joint Surgery. British volume 86-B, no. 6 (August 2004): 797–800. http://dx.doi.org/10.1302/0301-620x.86b6.14783.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Chalón, Miriam C., Victoria Terán, Mario E. Arena, Rubén Oliszewki, and Silvia N. González. "Microbiological culture broth designed from food waste." Journal of Environmental Management 115 (January 2013): 1–4. http://dx.doi.org/10.1016/j.jenvman.2012.10.005.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Lee, Chong-Suh, Kyung-Chung Kang, Sung-Soo Chung, Ki-Tack Kim, and Seong-Kee Shin. "Incidence of microbiological contamination of local bone autograft used in posterior lumbar interbody fusion and its association with postoperative spinal infection." Journal of Neurosurgery: Spine 24, no. 1 (January 2016): 20–24. http://dx.doi.org/10.3171/2015.3.spine14578.

Full text

Abstract:

OBJECT The aim of this study was to examine the results of microbiological cultures from local bone autografts used in posterior lumbar interbody fusion (PLIF) and to identify their association with postoperative spinal infection. METHODS The authors retrospectively evaluated cases involving 328 patients who had no previous spinal surgeries and underwent PLIF for degenerative diseases with a minimum 1-year follow-up. Local bone was obtained during laminectomy, and microbiological culture was performed immediately prior to bone grafting. The associations between culture results from local bone autografts and postoperative spinal infections were evaluated. RESULTS The contamination rate of local bone was 4.3% (14 of 328 cases). Coagulase-negative Staphylococcus (29%) was the most common contaminant isolated, followed by Streptococcus species and methicillin-sensitive Staphylococcus aureus. Of 14 patients with positive culture results, 5 (35.7%) had postoperative spinal infections and were treated with intravenous antibiotics for a minimum of 4 weeks. One of these 5 patients also underwent reoperation for debridement during this 4-week period. Regardless of the microbiological culture results, the infection rate after PLIF with local bone autograft was 2.4% (8 of 328 cases), with 5 (62.5%) of 8 patients showing positive results on autograft culture. CONCLUSIONS The incidence of contamination of local bone autograft during PLIF was considerable, and positive culture results were significantly associated with postoperative spinal infection. Special attention focused on the preparation of local bone for autograft and its microbiological culture will be helpful for the control of postoperative spinal infection.

APA, Harvard, Vancouver, ISO, and other styles

8

Soares, Janir Alves, and Donaldo Rosa Pires Júnior. "Influence of sodium hypochlorite-based irrigants on the susceptibility of intracanal microbiota to biomechanical preparation." Brazilian Dental Journal 17, no. 4 (2006): 310–16. http://dx.doi.org/10.1590/s0103-64402006000400009.

Full text

Abstract:

This study evaluated the microbiological conditions of root canals, using smears and culture from anterior teeth and premolars with necrotic pulps associated with chronic periapical pathologies, before and after biomechanical preparation (BMP). During double-flared instrumentation, 1, 2.5 and 5% sodium hypochlorite (NaOCl)-based irrigants were used in 3 groups: GI (n=39), GII (n=36) and GIII (n=36), respectively. Before BMP, all cultures were positive and the smears showed microbiologically diverse morphotypes, including fusiforms, pleomorphic, rods, cocci and filaments. Quantitetively, 20, 20 and 23 morphotypes were identified in GI, GII and GIII, respectively). After BMP, the percentages of negative cultures in GI, GII and GIII were 74.2%, 86.3% and 93.4% (p>0.05) and the number of morphotypes decreased to 14, 15 and 5, respectively. All teeth with 2 root canals and/or associated fistulas were microbiologically negative after BMP, regardless of irrigant concentration. Gram-negative morphotypes were more susceptible to the action of irrigants. After irrigation with 5% NaOCl, only structural arrangements consisting of Gram-positive cocci and bacilli persisted. Thus, BMP plus 5% NaOCl offered the best antiseptic potential because in the few positive cultures a significant reduction in the number of microbiological morphotypes was also shown (p<0.05).

APA, Harvard, Vancouver, ISO, and other styles

9

Tomanovic, Branka, and Veljko Mirovic. "Frequency and colonization rate of intravascular catheters." Vojnosanitetski pregled 61, no. 3 (2004): 255–58. http://dx.doi.org/10.2298/vsp0403255t.

Full text

Abstract:

Clinical signs are not sufficiently reliable for establishing diagnosis of intravascular catheter-related infection. Therefore, microbiological confirmation, based on the culture of the catheter tip after its removal, is necessary in diagnosing the infection. The aim of this study was to determine the frequency and the degree of microbial colonization of intravascular catheters (IVK), and the risk for the onset of sepsis, by using qualitative, semiquantitative (roll plate) and quantitative (vortexing) catheter culture techniques. During the period April 2001 December 2002, 289 intravascular catheters were cultured. A total of 284 microorganisms were isolated from 217 (75%) culture-positive catheters. The frequency of isolation of some organisms was the following coagulase-negative staphylococci (CNS) 41%, Staphylococcus aureus 19% Enterococci spp 6%, other Gram-positive microorganisms 9%, Gram-negative microorganisms 21%, and fungi 4%. In 35 catheters, cultures were polymicrobial; two microorganisms were found in 25 cultures and three were found to be in 10 cultures. There were 122 (46%) intravascular catheters which were found significantly colonized. A high rate of positivity and a high rate of S. aureus isolates and Gram-negative bacteria indicate the need of establishing the exact microbiological diagnosis of these infections, and the rigorous undertaking of adequate control and preventive measures.

APA, Harvard, Vancouver, ISO, and other styles

10

Zelus, Casey, Michael Blaha, Jasmine R. Marcelin, Jasmine R. Marcelin, Kelly Cawcutt, Kelly Cawcutt, Andre C. Kalil, and Kaeli Samson. "2204. Microbiology of Pneumonia Due to Co-Infection in the ICU: Impact of Host Immune Status." Open Forum Infectious Diseases 6, Supplement_2 (October 2019): S751—S752. http://dx.doi.org/10.1093/ofid/ofz360.1884.

Full text

Abstract:

Abstract Background Pneumonia epidemiology is increasingly showing the presence of co-infection due to the utilization of emerging diagnostic testing modalities such as multiplex polymerase chain reaction (PCR) panels. However, the prevalence and clinical significance of co-infection with respect to host immune status remain unclear. Methods A single-center retrospective analysis of mechanically ventilated adult patients treated in critical care units from January to October 2018 was performed on those with positive microbiological analysis of a bronchoalveolar lavage (BAL) sample. Host immune status and microbiological analyses were obtained including PCR and culture testing. Categorical variables and co-infection or immunocompetent status were assessed using Chi-Square, Fisher exact tests, or t-tests. REDCap was utilized for data abstraction and SAS software version 9.4 was used to perform all analysis. Results Of the 139 BAL samples that met inclusion criteria, 107 and 32 were obtained from immunocompetent and immunocompromised hosts, respectively. There was no statistical difference found between the frequency of co-infection detected by BAL culture with respect to host immune status. Immunocompetent patients had a higher proportion of positive bacterial cultures compared with immunocompromised (76.7% vs. 43.8% respectively, P = 0.0004). There was no significant difference seen with frequency of fungal or acid fast bacilli cultures between the two groups. Analysis of the microbiologic data obtained (figures) revealed different pathogens according to host immune status. Conclusion Pneumonia due to co-infection in critically ill, mechanically ventilated immunocompromised hosts occurs at a similar frequency regardless of host immune status, however different microbiological patterns emerge. Interestingly, patients who were not immunocompromised had a higher proportion of positive bacterial cultures compared with those who were immunocompromised. Comparative analysis of the other pathogen types may also reveal differences in detection rates if sample size is increased. Clinically, this may help guide efficient use of microbiological testing among patients based on immune status. Disclosures All authors: No reported disclosures.

APA, Harvard, Vancouver, ISO, and other styles

11

Yagupsky, Pablo. "Microbiological Diagnosis of Skeletal System Infections in Children." Current Pediatric Reviews 15, no. 3 (December 9, 2019): 154–63. http://dx.doi.org/10.2174/1573396315666190408114653.

Full text

Abstract:

Background:If not timely diagnosed and adequately treated, skeletal system infections in children may result in severe and permanent disability. Prompt identification of the etiology of the disease and determination of its antibiotic susceptibility are crucial for the successful management of septic arthritis, osteomyelitis, and spondylodiscitis. However, the bacteriological diagnosis of these infections has been traditionally limited by the low yield of conventional cultures and, on average, one-third of cases of pediatric joint and bone infections remained unconfirmed.Objective:To review the medical literature to summarize the current approach diagnosing the pediatric skeletal system infections.Methods:The relevant publications for the last three decades were reviewed.R:In recent years, the detection of skeletal system pathogens has been revolutionized by the use of improved laboratory methods, including seeding of synovial fluid and bone exudates into blood culture vials, and the development and implementation of sensitive nucleic acid amplification assays. These advances have resulted in the recognition of Kingella kingae as the predominant etiology of hematogenous infections of bones, joints, intervertebral discs and tendon sheaths in children aged 6-48 months, and reduced the fraction of culture-negative osteoarthritis.:As the exudate and tissue samples obtained from young children with skeletal system infections are frequently insufficient for a comprehensive laboratory workup, physicians should take in consideration the patient’s age, predisposing medical conditions and possible exposure to zoonotic organisms, and use a judicious combination of Gram’s stain, culture on blood culture vials, and molecular tests to maximize the microbiological diagnosis of these diseases.

APA, Harvard, Vancouver, ISO, and other styles

12

Nasrallah, Yasser, Maha Anani, Hanan Omar, and Asmaa Hashem. "Microbiological profiles of semen culture in male infertility." Human Andrology 8, no. 2 (June 1, 2018): 34–42. http://dx.doi.org/10.21608/ha.2018.3207.1023.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Premaratne, R. J., and M. A. Cousin. "Microbiological Analysis and Starter Culture Growth in Retentates." Journal of Dairy Science 74, no. 10 (October 1991): 3284–92. http://dx.doi.org/10.3168/jds.s0022-0302(91)78514-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Puchner, Stephan E., Kevin Döring, Kevin Staats, Christoph Böhler, Richard Lass, Alexander M. Hirschl, Elisabeth Presterl, Reinhard Windhager, and Johannes Holinka. "Sonication culture improves microbiological diagnosis of modular megaprostheses." Journal of Orthopaedic Research 35, no. 7 (September 19, 2016): 1383–87. http://dx.doi.org/10.1002/jor.23406.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Tabatabaei, Seyed Ali, Mohammad Soleimani, Reza Mirshahi, Bahram Bohrani, and Mehdi Aminizade. "Culture-proven endogenous endophthalmitis: microbiological and clinical survey." International Ophthalmology 40, no. 12 (August 2, 2020): 3521–28. http://dx.doi.org/10.1007/s10792-020-01540-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Cohen, Daniel, Ayman Natshe, Eli Ben Chetrit, Ehud Lebel, and Gabriel S. Breuer. "Synovial fluid culture: agar plates vs. blood culture bottles for microbiological identification." Clinical Rheumatology 39, no. 1 (September 6, 2019): 275–79. http://dx.doi.org/10.1007/s10067-019-04740-w.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Niedzielski, Artur, Lechosław Paweł Chmielik, and Tomasz Stankiewicz. "The Formation of Biofilm and Bacteriology in Otitis Media with Effusion in Children: A Prospective Cross-Sectional Study." International Journal of Environmental Research and Public Health 18, no. 7 (March 30, 2021): 3555. http://dx.doi.org/10.3390/ijerph18073555.

Full text

Abstract:

Background: Otitis media with effusion (OME) can cause serious complications such as hearing impairment or development delays. The aim of the study was to assess the microbiological profile of organisms responsible for OME and to determine if a biofilm formation can be observed. Methods: Ninety-nine samples from 76 patients aged from 6 months to 12 years were collected for microbiological and molecular studies. Results: In microbiological studies, pathogenic bacteria Haemophilus influenzae (38.89%), Streptococcus pneumoniae (33.33%), and Staphylococcus aureus MSSA (27.78%), as well as opportunistic bacteria Staphylococcus spp. (74.14%), Diphtheroids (20.69%), Streptococcus viridans (3.45%), and Neisseria spp. (1.72%) were found. The average degree of hearing loss in the group of children with positive bacterial culture was 35.9 dB, while in the group with negative bacterial culture it was 25.9 dB (p = 0.0008). The type of cultured bacteria had a significant impact on the degree of hearing impairment in children (p = 0.0192). In total, 37.5% of Staphylococcus spp. strains were able to form biofilm. Conclusions: Staphylococcus spp. in OME may form biofilms, which can explain the chronic character of the disease. Pathogenic and opportunistic bacteria may be involved in the etiopathogenesis of OME. The degree of hearing loss was significantly higher in patients from which the positive bacterial cultures were obtained.

APA, Harvard, Vancouver, ISO, and other styles

18

Arismendi-Morillo, Gabriel, Ileana Hernández, Edgardo Mengual, Alisbeth Fuenmayor, Gisela Romero, and Maribel Lizarzábal. "Comparison of three methods based on endoscopic gastric biopsies for diagnosis of Helicobacter pylori active infection in a clinical setting." Arquivos de Gastroenterologia 48, no. 3 (September 2011): 190–94. http://dx.doi.org/10.1590/s0004-28032011000300007.

Full text

Abstract:

CONTEXT: The correct diagnosis and effective treatment of Helicobacter pylori gastric infection are essential in controlling this infection. OBJECTIVE: To compare the diagnostic value of three tests based in endoscopic gastric biopsies histopathological evaluation with hematoxylin-eosin (H-E) staining, urease rapid test and microbiological culture for detecting Helicobacter pylori active infection, in order to make recommendations for daily clinical practice. METHODS: Gastric biopsies from 115 adult patients (85 female/30 male) were obtained by upper gastrointestinal endoscopy and studied by histopathological evaluation with H-E (antrum-corpus), urease test in 2 hours (antrum) and microbiological culture (antrum). RESULTS: Helicobacter pylori active infection was diagnosed in 67% of patients. Helicobacter pylori active infection was detected by histopathological evaluation with H-E, urease test and microbiological culture in 87%, 79% and 70% of the positive cases, respectively. There were significant differences when histopathological evaluation with H-E and urease test rapid test when compared with microbiological test (P<0.01). There was no significant difference between histopathological evaluation with H-E and urease test (P = 0.7). The kappa index of agreement for histopathological evaluation with H-E/urease test was 0.56, histopathological evaluation with H-E/microbiological culture 0.6, and urease test/microbiological culture 0.64. CONCLUSIONS: In a hospital setting like the one studied, histopathological evaluation with H-E and urease test are the most recommended tests for diagnosis of Helicobacter pylori active infection based in endoscopic biopsies. If pathological information of gastric lesions will be required, histopathological evaluation with H-E is essential. Urease test is mandatory if a prompt diagnosis is necessary. Microbiological culture can be used in cases of persistent or complicated infection, which may require studies on Helicobacter virulence or antimicrobial susceptibility. Selected cases might demand a combination of several tests. The three tests exhibit a good concordance level for Helicobacter pylori active infection diagnosis.

APA, Harvard, Vancouver, ISO, and other styles

19

Hedrich, Sabrina, Elke Heinzel, Jana Seifert, and Michael Schlömann. "Isolation of Novel Iron-Oxidizing Bacteria from an Acid Mine Water Treatment Plant." Advanced Materials Research 71-73 (May 2009): 125–28. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.125.

Full text

Abstract:

The capacity of a microbiological mine water treatment plant may to be enhanced by understanding the microbiological processes. Therefore different samples from the pilot plant were analyzed by culture-independent and cultivation methods. Dominant bacteria could be isolated on overlay plates or enriched in gradient cultures. To immobilize biomass in the pilot plant, various carrier materials were tested. Sessil, the material currently used in the pilot plant, was the most favored and appropriate material.

APA, Harvard, Vancouver, ISO, and other styles

20

Elsayed, Sameer, Daniel B. Gregson, Tracie Lloyd, Marilyn Crichton, and Deirdre L. Church. "Utility of Gram Stain for the Microbiological Analysis of Burn Wound Surfaces." Archives of Pathology & Laboratory Medicine 127, no. 11 (November 1, 2003): 1485–88. http://dx.doi.org/10.5858/2003-127-1485-uogsft.

Full text

Abstract:

Abstract Context.—Surface swab cultures have attracted attention as a potential alternative to biopsy histology or quantitative culture methods for microbiological burn wound monitoring. To our knowledge, the utility of adding a Gram-stained slide in this context has not been evaluated previously. Objective.—To determine the degree of correlation of Gram stain with culture for the microbiological analysis of burn wound surfaces. Design.—Prospective laboratory analysis. Setting.—Urban health region/centralized diagnostic microbiology laboratory. Patients.—Burn patients hospitalized in any Calgary Health Region burn center from November 2000 to September 2001. Intervention.—Gram stain plus culture of burn wound surface swab specimens obtained during routine dressing changes or based on clinical signs of infection. Main Outcome Measures.—Degree of correlation (complete, high, partial, none), including weighted κ statistic (κw), of Gram stain with culture based on quantitative microscopy and degree of culture growth. Results.—A total of 375 specimens from 50 burn patients were evaluated. Of these, 239 were negative by culture and Gram stain, 7 were positive by Gram stain only, 89 were positive by culture only, and 40 were positive by both methods. The degree of complete, high, partial, and no correlation of Gram stain with culture was 70.9% (266/375), 1.1% (4/375), 2.4% (9/375), and 25.6% (96/375), respectively. The degree of correlation for all 375 specimens, as expressed by the weighted κ statistic, was found to be fair (κw = 0.32). Conclusion.—The Gram stain is not suitable for the microbiological analysis of burn wound surfaces.

APA, Harvard, Vancouver, ISO, and other styles

21

Waters, Sinéad M., Richard A. Murphy, and Ronan F. G. Power. "Characterisation of prototype Nurmi cultures using culture-based microbiological techniques and PCR-DGGE." International Journal of Food Microbiology 110, no. 3 (August 2006): 268–77. http://dx.doi.org/10.1016/j.ijfoodmicro.2006.04.028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Traynor, Peter. "Culture Media SIG." Microbiology Australia 33, no. 4 (2012): 000. http://dx.doi.org/10.1071/ma12903.

Full text

Abstract:

The Culture Media Special Interest Group of the Australian Society for Microbiology was formed in 1991 by a group of interested individuals after an upsurge in interest in the issue of media quality and the appearance that no common standards or consensus existed in this area in Australia. Increased interest, especially amongst medical microbiologists, in what was being done, or should be done, by way of assuring the quality of microbiological media made the issue contentious.

APA, Harvard, Vancouver, ISO, and other styles

23

Madani, Devangi Ketankumar, Mujahid Ahmad Saeed, Alok Tiwari, and Miruna Delia David. "The role of remnant bone microbiological cultures in managing diabetic foot osteomyelitis." British Journal of Diabetes 21, no. 1 (May 28, 2021): 59–61. http://dx.doi.org/10.15277/bjd.2021.285.

Full text

Abstract:

Aim: Effective treatment of diabetic foot osteomyelitis can reduce the risk of major amputations. Our primary aim was to compare the yield in cultures from the proximal and distal segments of bone excised intraoperatively and the impact on antibiotic choice and duration.Methods: Patients with a confirmed diagnosis of osteomyelitis on bone culture results, where both proximal and distal bone segment samples had been collected, were retrospectively reviewed. Microbiological data were examined to identify true pathogens and studied against antimicrobial choice and duration of prescribing.Results: A total of 47 forefoot amputation cases were studied. In 89% of cases, definite or likely pathogens were isolated from the deep tissues cultured. Definite pathogens (Staphylococcus aureus, Group B streptococcus, Group G streptococcus and Streptococcus anginosus) were identified in 32% of cases; in 73% of these, definite pathogens were grown in both the proximal and distal bone segments.Conclusion: Sampling of remnant bone culture can help in reducing the duration of antibiotic treatment in patients (27% of cases in our series) as it is challenging to correctly estimate intraoperatively whether clear surgical margins have been adequately achieved when resecting infected bone.

APA, Harvard, Vancouver, ISO, and other styles

24

Girmenia, Corrado. "Microbiological diagnostics of fungal infections." Reviews in Health Care 4, no. 1S (July 11, 2013): 33–37. http://dx.doi.org/10.7175/rhc.v4i1s.862.

Full text

Abstract:

Laboratory tests for the detection of fungal infections are easy to perform. The main obstacle to a correct diagnosis is the correlation between the laboratory findings and the clinical diagnosis. Among pediatric patients, the most common fungal pathogen is Candida. The detection of fungal colonization may be performed through the use of chromogenic culture media, which allows also the identification of Candida subspecies, from which pathogenicity depends. In neonatology, thistest often drives the decision to begin a empiric therapy; in this regard, a close cooperation between microbiologists and clinicians is highly recommended. Blood culture, if positive, is a strong confirmation of fungal infection; however, its low sensitivity results in a high percentage of false negatives, thus decreasing its reliability. Molecular diagnostics is still under evaluation, whereas the detection of some fungal antigens, such as β-D-glucan, galactomannan, mannoprotein, and cryptococcal antigen in the serum is used for adults, but still under evaluations for pediatric patients.

APA, Harvard, Vancouver, ISO, and other styles

25

Frank, Daniel N., Shandra S. Wilson, Allison L. St. Amand, and Norman R. Pace. "Culture-Independent Microbiological Analysis of Foley Urinary Catheter Biofilms." PLoS ONE 4, no. 11 (November 12, 2009): e7811. http://dx.doi.org/10.1371/journal.pone.0007811.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

James, Ray. "MICROBIOLOGICAL MONITORING OF DIALYSIS WATER SYSTEMS - WHICH CULTURE METHOD?" Journal of Renal Care 33, no. 2 (April 6, 2007): 66–69. http://dx.doi.org/10.1111/j.1755-6686.2007.tb00042.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Kim, Eugene Y., Anne V. Grossestreuer, Charles Safran, Larry A. Nathanson, and Steven Horng. "A visual representation of microbiological culture data improves comprehension: a randomized controlled trial." Journal of the American Medical Informatics Association 28, no. 9 (June 8, 2021): 1826–33. http://dx.doi.org/10.1093/jamia/ocab056.

Full text

Abstract:

Abstract Objective While the judicious use of antibiotics takes past microbiological culture results into consideration, this data’s typical format in the electronic health record (EHR) may be unwieldy when incorporated into clinical decision-making. We hypothesize that a visual representation of sensitivities may aid in their comprehension. Materials and Methods A prospective parallel unblinded randomized controlled trial was undertaken at an academic urban tertiary care center. Providers managing emergency department (ED) patients receiving antibiotics and having previous culture sensitivity testing were included. Providers were randomly selected to use standard EHR functionality or a visual representation of patients’ past culture data as they answered questions about previous sensitivities. Concordance between provider responses and past cultures was assessed using the kappa statistic. Providers were surveyed about their decision-making and the usability of the tool using Likert scales. Results 518 ED encounters were screened from 3/5/2018 to 9/30/18, with providers from 144 visits enrolled and analyzed in the intervention arm and 129 in the control arm. Providers using the visualization tool had a kappa of 0.69 (95% CI: 0.65–0.73) when asked about past culture results while the control group had a kappa of 0.16 (95% CI: 0.12–0.20). Providers using the tool expressed improved understanding of previous cultures and found the tool easy to use (P < .001). Secondary outcomes showed no differences in prescribing practices. Conclusion A visual representation of culture sensitivities improves comprehension when compared to standard text-based representations.

APA, Harvard, Vancouver, ISO, and other styles

28

van Samkar, Ganapathy, Payal P. S. Balraadjsing, Henning Hermanns, Irene V. Hoogendijk, Markus W. Hollmann, Sebastian A. J. Zaat, and Markus F. Stevens. "Microbiological and scanning electron microscopic evaluation of epidural catheters." Regional Anesthesia & Pain Medicine 45, no. 5 (March 15, 2020): 381–85. http://dx.doi.org/10.1136/rapm-2019-101180.

Full text

Abstract:

BackgroundEpidural catheters are frequently colonized by gram-positive bacteria. Although the incidence of associated epidural infections is low, their consequences can be devastating. We investigated bacterial growth on epidural catheters by quantitative bacterial culture and scanning electron microscopy (SEM) in order to explore the patterns of epidural catheter colonization.Methods28 patients undergoing major abdominal surgery with thoracic epidurals (treatment ≥72 hours) were studied. Before the removal of the catheter, the skin surrounding the insertion site was swabbed. The entire catheter was divided into extracorporeal, subcutaneous, and tip segments. Skin swabs and catheter segments were quantitatively cultured, bacterial species were identified, and SEM was performed on four selected catheters.Results27 of 28 catheters were included. The percentages of positive cultures were: skin swab 29.6%, extracorporeal segments 11.1%, subcutaneous segments 14.8%, and tip segments 33.3%. One patient was diagnosed with a catheter-associated infection. Staphylococcus epidermidis was cultured from the skin and the catheter extracorporeal, subcutaneous, and tip segments. SEM of this catheter showed bacteria-like and intraluminal host cell-like structures. SEM of two other catheters showed intraluminal fibrin networks in their tip segments.ConclusionsWe present the first SEM pictures of an epidural catheter with a bacterial infection. Bacterial growth developed from the skin to the tip of this catheter, indicating the skin as a primary source of infection. By SEM, catheters with low levels of bacterial growth demonstrated an intraluminal fibrous network which possibly plays a role in catheter obstruction.

APA, Harvard, Vancouver, ISO, and other styles

29

Mitnick, Carole D., Richard A. White, Chunling Lu, Carly A. Rodriguez, Jaime Bayona, Mercedes C. Becerra, Marcos Burgos, et al. "Multidrug-resistant tuberculosis treatment failure detection depends on monitoring interval and microbiological method." European Respiratory Journal 48, no. 4 (September 1, 2016): 1160–70. http://dx.doi.org/10.1183/13993003.00462-2016.

Full text

Abstract:

Debate persists about monitoring method (culture or smear) and interval (monthly or less frequently) during treatment for multidrug-resistant tuberculosis (MDR-TB). We analysed existing data and estimated the effect of monitoring strategies on timing of failure detection.We identified studies reporting microbiological response to MDR-TB treatment and solicited individual patient data from authors. Frailty survival models were used to estimate pooled relative risk of failure detection in the last 12 months of treatment; hazard of failure using monthly culture was the reference.Data were obtained for 5410 patients across 12 observational studies. During the last 12 months of treatment, failure detection occurred in a median of 3 months by monthly culture; failure detection was delayed by 2, 7, and 9 months relying on bimonthly culture, monthly smear and bimonthly smear, respectively. Risk (95% CI) of failure detection delay resulting from monthly smear relative to culture is 0.38 (0.34–0.42) for all patients and 0.33 (0.25–0.42) for HIV-co-infected patients.Failure detection is delayed by reducing the sensitivity and frequency of the monitoring method. Monthly monitoring of sputum cultures from patients receiving MDR-TB treatment is recommended. Expanded laboratory capacity is needed for high-quality culture, and for smear microscopy and rapid molecular tests.

APA, Harvard, Vancouver, ISO, and other styles

30

Pandey, Jagat S. "Analysis of Microbiological Culture Specimens in Shree Birendra Hospital, Chhauni." Medical Journal of Shree Birendra Hospital 6 (December 1, 2003): 7–13. http://dx.doi.org/10.3126/mjsbh.v6i0.21105.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Kehrmann, Jan, Valerie Chapot, Jan Buer, Philipp Rating, Norbert Bornfeld, and Joerg Steinmann. "Diagnostic performance of blood culture bottles for vitreous culture compared to conventional microbiological cultures in patients with suspected endophthalmitis." European Journal of Clinical Microbiology & Infectious Diseases 37, no. 5 (January 9, 2018): 889–95. http://dx.doi.org/10.1007/s10096-017-3182-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Eslami, Fatemeh, Hamid Reza Ghasemi Basir, Abbas Moradi, and Mina Bayat. "Microbiological analysis of contact lens cases and effective health behaviors." Immunopathologia Persa 7, no. 2 (September 6, 2020): e17-e17. http://dx.doi.org/10.34172/ipp.2021.17.

Full text

Abstract:

Introduction: Contact lenses are increasingly being used for cosmetic or therapeutic purposes, followed by subsequent contamination and complications such as keratitis. The lens case is one of the most common places to find the cause of contamination. Objectives: This study was designed to evaluate the health behaviors affecting the lens case contamination and its relationship with the result of lens case culture which can help in prevention of complications. Patients and Methods: In this cross-sectional study that was performed in northwest of Iran, 150 asymptomatic participants were assessed for health behaviors affecting the lens case contamination and their lens cases were sampled for culture and antibiogram. Data were analyzed with SPSS-16 software. Results: The frequency of positive microbial culture in medical and cosmetic contact lens cases was 30.7% and 66.8%, respectively and 32.7% in general. Among the isolated bacteria observed in positive cultures, Alcaligenes,Enterobacter aerogenes, gram-positive Diphtheroid bacilli, Pseudomonas aeruginosa and Staphylococcus epidermidis were the most common microorganisms, respectively. Conclusion: A significant proportion of contact lens cases, especially those used for cosmetic purposes had bacterial contamination. Failure to replace the lens case for more than 9 months and the mismatch of the lens solution brand with its storage case will increase bacterial contamination. Washing the lens case with soap and water, and drying it after washing, will reduce bacterial contamination.

APA, Harvard, Vancouver, ISO, and other styles

33

Khan, Muhammad N., Raghavan Vidya, and Richard E. Lee. "The Limited Role of Microbiological Culture and Sensitivity in the Management of Superficial Soft Tissue Abscesses." Scientific World JOURNAL 6 (2006): 1118–23. http://dx.doi.org/10.1100/tsw.2006.215.

Full text

Abstract:

The aim of this study was to assess the role of the routine practice of microbial culture and sensitivity at incision and drainage of superficial soft tissue abscesses. The case notes of 162 consecutive patients, selected from the microbiology database over a period of 1 year, were reviewed. All had incision and drainage of superficial soft tissue abscesses and included perianal, pilonidal, axillary, and breast abscesses. Patients with chronic wounds, recurrent abscesses, diabetes, pregnancy, and immunosuppression were excluded. The impact of pus culture and sensitivity (C/S) on management and clinical outcome was documented. Out of 162 patients, 97 were male (59.8%) and 65 were female (40.1%). Only 115 (70.9%) yielded positive cultures and 47 (29.1%) were sterile. The cultured microbial flora was predictable and sensitive to empirical antibiotics. In four patients, the results of microbial culture sensitivity showed microbial resistance to empirical antibiotics; however, it did not affect the management or the outcome for these patients. The routine practice of sending swabs for C/S after incision and drainage of superficial soft tissue abscesses does not contribute significantly towards patient management. Most patients are already on antibiotics prior to the referral and in the remainder, surgeons start antibiotics empirically. These broad-spectrum antibiotics cover the common pathogens involved, and there is no significant change in the antibiotic treatment after reviewing the culture reports following incision and drainage of uncomplicated superficial skin abscesses.

APA, Harvard, Vancouver, ISO, and other styles

34

Günther, Susanne-Katharina, Celina Geiss, Stefan J. Kaiser, Nico T. Mutters, and Frank Günther. "Microbiological Control of Cellular Products: The Relevance of the Cellular Matrix, Incubation Temperature, and Atmosphere for the Detection Performance of Automated Culture Systems." Transfusion Medicine and Hemotherapy 47, no. 3 (October 22, 2019): 254–63. http://dx.doi.org/10.1159/000503397.

Full text

Abstract:

Background: The microbiological control of cellular products sometimes causes significant procedural issues for quality control laboratories. According to the European Pharmacopoeia (EP), the microbiological control of cellular products requires a 7- to 14-day incubation period at two different incubation temperatures using aerobic and anaerobic growth media. However, the suitability of these test conditions for efficient quality control can be influenced by many conditions, such as the expected microbial spectrum of contamination or the texture and composition of the cellular product. Because of interference, direct inoculation and membrane filtration as reference methods of pharmacopoeia are largely unsuitable for the microbiological control of cellular products; therefore, alternative and, above all, automated methods are the focus of interest. Objective: The aim of our study was to evaluate the method suitability and possible effects of cell matrix, incubation temperature, and oxygen pressure on the detection performance of automated culture systems. Methods: The BacT/ALERT® 3DTM Dual T system (bioMérieux, Nürtingen, Germany) was used to evaluate the factors influencing automated microbiological control of cellular products. The tests were performed using microbial strains recommended by the EP for microbiological method suitability testing and additional relevant possible contaminants of human-derived stem-cell products under varying culture and cell matrix conditions. Results: All contaminants were detected by the system in the required period of 2–5 days. Low incubation temperatures (22°C) had overall negative effects on the detection kinetics of each type of microbial contamination. The adverse effects of the accompanying cell matrix on the detection properties of the system could be compensated in our study by incubation at 32°C in both the aerobic and the anaerobic culture conditions. Conclusion: Automated culture techniques represent a sufficient approach for the microbiological control of cellular products. The negative effects of the cell matrix and microbial contamination on the detection performance can be compensated by the application of variable culture conditions in the automated culture system.

APA, Harvard, Vancouver, ISO, and other styles

35

Lempk, Marcus Welbert, Afonso de Liguori Oliveira, Roberto Gonçalves Junqueira, Eliara Acipreste Hudson, Ana Clarissa dos Santos Pires, Daniel Arantes Pereira, Kely Tatianne Costa Santana, Roberta Ribeiro da Cruz Cangussu, Janaína Teles de Faria, and Maximiliano Soares Pinto. "Natural starter culture on artisanal cheese." Research, Society and Development 9, no. 10 (September 27, 2020): e3039108604. http://dx.doi.org/10.33448/rsd-v9i10.8604.

Full text

Abstract:

The use of natural starter culture in the manufacture of artisanal Minas cheeses is required by the government legislation in Brazil. However, there are no scientific support on role of the natural starter culture in these cheeses. The aim of this work was to compare artisanal Minas cheeses produced with and without natural starter culture to verify the influence on the physico-chemical and microbiological characteristics during the ripening process. The use of natural starter culture did not have a significant effect on the physico-chemical (dry matter, humidity, fat, pH, chlorides, water activity, ash, total protein, depth and extent of proteolysis) and microbiological (Escherichia coli, coliforms and Staphylococcus aureus) properties of the cheeses. The significant changes that occurred in the cheeses were mainly because of the ripening time. According to obtained results, natural starter has shown to be innocuous to traditional Minas Serro cheese. The present study is pioneering in the scientific literature and opens new horizons for the understanding of the requirement to use natural starter culture.

APA, Harvard, Vancouver, ISO, and other styles

36

Kaya, Esingül, Lara Tollapi, Alberto Pastore, Giacomo Aringhieri, Giuseppantonio Maisetta, Simona Barnini, Adriana Paolicchi, Giovanna Batoni, and Semih Esin. "Comparison of methods for the microbiological diagnosis of totally implantable venous access port-related infections." Journal of Medical Microbiology 69, no. 11 (November 1, 2020): 1273–84. http://dx.doi.org/10.1099/jmm.0.001263.

Full text

Abstract:

Introduction. Totally implanted venous access ports (TIVAPs) are widely used in patients receiving long-term chemotherapy but may lead to serious complications such as catheter-related bloodstream infections (CRBSIs). Diagnosis of CRBSI requires catheter culture, but there is no consensus on microbiological culture methods to be adopted. Aim. To compare three different procedures to recover bacterial cells from colonized catheters and to determine which section of the TIVAP (i.e. tip, septum, reservoir) is the probable source of infection. To investigate the correlation between blood culture results and TIVAP culture in order to get further evidence about the utility of differential time to positivity (DTP) as a diagnostic tool before TIVAP removal. Hypothesis/Gap statement. Comparisons of different diagnostic procedures for catheter culture have been rarely reported for TIVAPs. We hypothesized that the optimization of methods to recover micro-organisms from different parts of TIVAPs may help to decrease the number of false-negative results in the diagnosis of TIVAP-related bloodstream infections. Methodology. A total of 53 TIVAPs removed because of suspected infection (n=36) or end of use (n=17) were evaluated. The reservoir, the septum and the catheter tip were separated and subjected to different treatments for the recovery of adherent micro-organisms: (a) flushing of the catheter lumen, (b) sonication and flushing, (c) treatment with dithiothreitol and flushing. The three methods were also evaluated in an in vitro catheter infection model with Staphylococcus epidermidis . Culture results were compared to those obtained from paired blood cultures drawn from TIVAP and peripheral vein and to the relative DTP. Results. The results obtained demonstrated that vigorous flushing/vortexing of the catheter lumen/septum, allows the recovery of a number of micro-organisms comparable to that of more complex procedures such as sonication or chemical treatment. Among 24 positive TIVAP-cultures, nine were tip-culture negative, whereas the corresponding reservoirs and septa were culture positive. A good correlation was observed between DTP and TIVAP cultures (P<0.001). Conclusions. The results support the evidence that sending the port reservoir in addition to the catheter tip to the microbiology laboratory may increase the sensitivity and the accuracy of CRBSI diagnosis. Moreover, when a TIVAP-related infection is suspected, DTP is a useful diagnostic tool to decide between device removal or a more conservative approach.

APA, Harvard, Vancouver, ISO, and other styles

37

Pasternak, Jacyr. "New methods of microbiological identification using MALDI-TOF." Einstein (São Paulo) 10, no. 1 (March 2012): 118–19. http://dx.doi.org/10.1590/s1679-45082012000100026.

Full text

Abstract:

Rapid diagnosis of pathogens is decisive to guarantee adequate therapy in infections: culture methods are precise and sensitive, but rather slow. New resources are available to enable faster diagnosis, and the most promising is MALDI-TOF technology: mass spectrometry applied to microbiological diagnosis. Times as fast as 10 to 15 minutes to etiological diagnosis are possible after a positive blood culture result. We hope to have this technology in our laboratory, ANVISA permitting and improving their very slow rate of doing things... MALDI-TOF is basically putting a sample of culture or an enriched suspension of the probable pathogen over a small spot with a matrix and vaporizing it with a laser pulse: the products are aspired into a chamber, ionized and reach detectors at variable times: the detectors show time of arrival and quantity of the product, and each pathogen has its characteristic spectrum analyzed by a software.

APA, Harvard, Vancouver, ISO, and other styles

38

Bhattacharyya, Neil. "Bacterial Infection in Chronic Rhinosinusitis: A Controlled Paired Analysis." American Journal of Rhinology 19, no. 6 (November 2005): 544–48. http://dx.doi.org/10.1177/194589240501900602.

Full text

Abstract:

Background The aim of this study was to determine if pathogenic bacteria are involved in the pathogenesis of chronic rhinosinusitis (CRS). Methods A consecutive series of adult patients with unilateral sinus disease determined by unilateral radiographic involvement or unilateral purulent secretions was microbiologically studied. Aerobic and anaerobic bacterial and fungal cultures were obtained during endoscopic sinus surgery from purulent secretions or tissue culture. Positive culture rates were compared between the diseased sinus and the contralateral nondiseased (control) sinus to determine if pathogenic bacteria were more commonly recovered from the diseased sinuses. Results Forty-nine adult patients completed the study with appropriate microbiological data. Coagulase-negative staphylococci were the most commonly recovered bacteria followed by Staphylococcus aureus from the diseased side of the sinuses with similar findings for the control sinus. Bacterial species were recovered from 87.8% of the diseased side of the sinuses versus 85.7% from the control sinuses (p = 0.50). Reanalysis with coagulase-negative staphylococci considered as non-pathogen showed a 46.9 and 49.0% positive bacterial culture rate in diseased and control groups, respectively (p = 0.50). No significant difference in positive anaerobic culture rates were identified between groups (59.1% diseased versus 55.1% control, respectively, p = 0.61). Antibiotic resistance rates were no different between bacteria cultured from diseased sinuses versus control (p = 0.115). Conclusion Both aerobic and anaerobic bacterial species may be recovered from both diseased and nondiseased sinuses in patients with CRS. These findings cast some doubt on the exact etiologic role of bacteria in CRS, suggesting other factors or other agents also may be responsible in CRS pathogenesis.

APA, Harvard, Vancouver, ISO, and other styles

39

Tiwari, Shalbha, Daliparthy D. Pratyush, Awanindra Dwivedi, Sanjiv K. Gupta, Madhukar Rai, and Surya K. Singh. "Microbiological and clinical characteristics of diabetic foot infections in northern India." Journal of Infection in Developing Countries 6, no. 04 (January 4, 2012): 329–32. http://dx.doi.org/10.3855/jidc.1827.

Full text

Abstract:

Introduction: India has the largest diabetic population of 50.8 million that could reach an epidemic proportion by 2030. Diabetic foot infection is one of the dreaded complications of diabetes. Only a few studies that focus on patterns of diabetic foot infection in our region, where diabetic foot care is inadequate, are available. This study evaluated microbial and clinical characteristics of diabetic foot infections that will be helpful in taking appropriate measures for their management. Methodology: In this prospective study conducted during 2008-2009, sixty-two diabetic foot patients underwent detailed history, clinical examination, and laboratory investigations including parameters of systemic infections. Microbial culture and sensitivity were performed at the time of presentation. Results: Among 62 cases, 43.5% had mono-microbial infection, 35.5% had poly-microbial infections, and 21% had sterile culture. Among 82 bacteria isolated, 68% were Gram negative and 32% were Gram positive. Leukocyte counts were higher (16928±9642 versus 14593±6687 cells/mm3) and haemoglobin (7.9±2.4 versus 9.2±2.2 mg/dl) lower in poly-microbial compared to mono-microbial infections. Haemoglobin counts were lower and leukocyte counts higher in Gram-negative compared to Gram-positive infections. Patients with sterile cultures also had clinical evidence of persistent infection. Escherichia coli were the most common isolate and piperacillin/tazobactam showed highest sensitivity. Conclusions: Gram-negative bacteria were most prevalent in diabetic foot infection. It is not uncommon to have culture reports negative despite clinical evidence of infection. This study suggests that piperacillin/tazobactam should be the treatment of choice on an empirical basis prior to a definitive bacteriological study and in cases with negative culture reports.

APA, Harvard, Vancouver, ISO, and other styles

40

Sendul, Selam Yekta, Sonmez Cinar, Halil Hüseyin Çağatay, Mehmet Demir, Burcu Dirim, and Dilek Guven. "Clinical, Radiological, Microbiological, and Histopathological Aspects of Acquired Dacryocystoceles." Journal of Ophthalmology 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/396782.

Full text

Abstract:

Purpose. The aim of this study is to investigate the etiology and the clinical, microbiological, histopathological, and radiological findings of acquired dacryocystoceles.Methods. In this retrospective study, we reviewed the clinical records of 10 eyes of 8 patients with dacryocystoceles who underwent external dacryocystorhinostomy (DCR) surgery. Etiology, presenting symptoms and radiological findings as well as microbiological and histopathological assessment results and outcome were analyzed.Results. The records of 8 patients with dacryocystoceles were included in this study. In the histopathological evaluations of the samples collected from the lacrimal sac wall, chronic inflammation was found in all biopsied samples and fibrosis was observed in two histopathological evaluations. Computerized tomography (CT) imaging showed fluid collection separated from adjacent tissues by a thin rim, corresponding to dacryocystoceles in the sac. In the microbiological culture examination of samples collected from the fluid within the cyst, no bacterial growth in 5 eyes, gram-negative bacillus growth in 3 eyes, and gram-positive cocci growth in 2 eyes were found.Conclusions. Acquired dacryocystoceles were observed extremely rarely and a definite pathogenic agent could not be identified in any of the cases, either microbiologically or histologically, whereas chronic inflammation was detected in all cases in our study.

APA, Harvard, Vancouver, ISO, and other styles

41

Brienze, Vânia Maria Sabadoto, Ângela Pedroso Tonon, Fabrício José Tarelho Pereira, Elisabete Liso, Waldir Antônio Tognola, Manoel Armando Azevedo dos Santos, and Marcelo Urbano Ferreira. "Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil." Revista da Sociedade Brasileira de Medicina Tropical 34, no. 4 (August 1, 2001): 389–93. http://dx.doi.org/10.1590/s0037-86822001000400015.

Full text

Abstract:

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

APA, Harvard, Vancouver, ISO, and other styles

42

Hoskisson, Paul A., and Glyn Hobbs. "Continuous culture – making a comeback?" Microbiology 151, no. 10 (October 1, 2005): 3153–59. http://dx.doi.org/10.1099/mic.0.27924-0.

Full text

Abstract:

The heyday of continuous culture was in the 1960s, when its versatility and reproducibility were used to address fundamental problems in diverse microbiological fields such as biochemistry, ecology, genetics and physiology. The advent of molecular genetics in the 1970s and 1980s led to a decline in the popularity of continuous culture as a standard laboratory tool. The current trend of studying global proteomics, transcriptomics and metabolomics requires reproducible, reliable and biologically homogeneous datasets with which to approach a given problem. The use of continuous culture techniques can aid the acquisition of such data, and continuous cultures offer advantages over biologically heterogeneous batch cultures, where secondary growth and stress effects can often mask subtle physiological differences and trends. This review is intended to remind microbiologists of the value of continuous cultivation in a wide range of biological investigations, and describes some advantages and recent advances in applications of continuous culture in post-genomic studies.

APA, Harvard, Vancouver, ISO, and other styles

43

Neeff, Michel, Kristi Biswas, Michael Hoggard, Michael W. Taylor, and Richard Douglas. "Molecular Microbiological Profile of Chronic Suppurative Otitis Media." Journal of Clinical Microbiology 54, no. 10 (August 3, 2016): 2538–46. http://dx.doi.org/10.1128/jcm.01068-16.

Full text

Abstract:

Chronic suppurative otitis media (CSOM) presents with purulent otorrhea (ear discharge), is characterized by chronic inflammation of the middle ear and mastoid cavity, and contributes to a significant disease burden worldwide. Current antibiotic therapy is guided by swab culture results. In the absence of detailed molecular microbiology studies of CSOM patients, our current understanding of the microbiota of CSOM (and indeed of the healthy ear) remains incomplete. In this prospective study, 24 patients with CSOM were recruited, along with 22 healthy controls. Culture-based techniques and 16S rRNA gene amplicon sequencing were used to profile the bacterial community for each patient. Comparisons between patients with and without cholesteatoma in the middle ear and mastoid cavity were also made. A major finding was that the middle ear of many healthy controls was not sterile, which is contradictory to the results of previous studies. However, sequencing data showed thatStaphylococcus aureus, along with a range of other Gram-positive and Gram-negative organisms, were present in all subgroups of CSOM and healthy controls. Large interpatient variability in the microbiota was observed within each subgroup of CSOM and controls, and there was no bacterial community “signature” which was characteristic of either health or disease. Comparisons of the culture results with the molecular data show that culture-based techniques underestimate the diversity of bacteria found within the ear. This study reports the first detailed examination of bacterial profiles of the ear in healthy controls and patients with CSOM.

APA, Harvard, Vancouver, ISO, and other styles

44

Carvalho, Isabel A., Vinicius E. B. Campos, Iana M. Souza, Larissa G. Zanardo, Jose D. Ribeiro Filho, Marcos J. P. Gomes, and Maria A. S. Moreira. "Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR." Brazilian Journal of Microbiology 43, no. 2 (June 2012): 581–85. http://dx.doi.org/10.1590/s1517-83822012000200020.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Cornish, K. S., S. Ramamurthi, I. Butcher, and K. Ramaesh. "Is Microbiological Analysis of Donor Cornea Transport Culture Media Necessary?" European Journal of Ophthalmology 19, no. 1 (January 2009): 137–38. http://dx.doi.org/10.1177/112067210901900121.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Mehmet Fatih, Yüzbaşıoğlu, Güzel Hatice, Çelik Gülay Oyur, Tuna Arzu, Benlier Necla, Topuz Sezgin, Peker Onur, and Boz Alper. "Microbiological culture results and antibiotic sensitivity in renal preservation solution." Archives of Clinical Nephrology 6, no. 1 (June 19, 2020): 020–23. http://dx.doi.org/10.17352/acn.000042.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Nakasaki, Kiyohiko, Mitsunori Yanagisawa, and Koji Kobayashi. "Microbiological quality of fermented milk produced by repeated-batch culture." Journal of Bioscience and Bioengineering 105, no. 1 (January 2008): 73–76. http://dx.doi.org/10.1263/jbb.105.73.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Janny, S., F. Bert, F. Dondero, F. Durand, P. Guerrini, P. Merckx, M. H. Nicolas-Chanoine, J. Belghiti, J. Mantz, and C. Paugam-Burtz. "Microbiological findings of culture-positive preservation fluid in liver transplantation." Transplant Infectious Disease 13, no. 1 (August 24, 2010): 9–14. http://dx.doi.org/10.1111/j.1399-3062.2010.00558.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Lopansri, Bert K., Tasha Fernley, Jana Coombs, Michaela A. Gazdik, Lori Smit, Kristin K. Dascomb, and John Burke. "1197. Microbiological Surveillance of Duodenoscopes Before and After High-Level Disinfection Following Endoscopic Retrograde Cholangiopancreatography (ERCP)." Open Forum Infectious Diseases 5, suppl_1 (November 2018): S362. http://dx.doi.org/10.1093/ofid/ofy210.1030.

Full text

Abstract:

Abstract Background Transmission of antibiotic-resistant bacteria during endoscopic retrograde cholangiopancreatography (ERCP) has been linked to the complex design of the duodenoscope (scope) elevator channel and cantilever. We implemented a scope culturing program to monitor the efficacy of disinfection and to identify frequency of pre-disinfection exposure to antibiotic-resistant bacteria. Methods Facilities performing ERCPs within the Intermountain Healthcare system voluntarily submit scope cultures to the Infectious Diseases Epidemiology Laboratory. Cultures are collected at designated intervals based on procedure volumes at each site. Samples are submitted by endoscopy techs trained to collect flush and swab samples of the distal end of the scope using a previously described method before (PRE) and after (POST) high-level disinfection. Selective media is used to screen for Gram-negative bacilli-resistant to third-generation cephalosporins (ESBL) and vancomycin-resistant Enterococcus (VRE). Results Between March 7, 2016 and April 18, 2018, 1,255 scope samples from 10 facilities were cultured (533 PRE samples and 722 POST samples). 483 (90.6%) PRE samples were positive, with 75 (15.5%) screening positive for an antibiotic-resistant organism (60 ESBL and 15 VRE). 19 (2.6%) POST samples were positive, with 4 (21.1%) screening positive for ESBL. One of the four ESBL positive POST samples had a corresponding PRE sample for comparison; E. coli and Klebsiella variicola were isolated in both indicating residual contamination. Two of the ESBL-positive POST cultures did not have corresponding PRE samples and one had a PRE culture negative for ESBL. No POST samples contained VRE. Endoscopy personnel were contacted for each positive POST culture and endoscopy reprocessing practices were reviewed. Additionally, scopes were quarantined, reprocessed and re-cultured. Scopes were returned to use once POST cultures were negative. Conclusion Contamination of scopes with antibiotic-resistant bacteria during ERCP is common. High-level disinfection is effective at reducing bacterial burden but is imperfect. Routine surveillance for post-reprocessing bacterial colonization has been helpful to minimize patient exposure and to maintain focus on the importance of reprocessing. Disclosures All authors: No reported disclosures.

APA, Harvard, Vancouver, ISO, and other styles

50

Maximov, I. S., N. G. Kochergin, V. S. Novoselov, Z. S. Ditmarova, and D. I. Ushakova. "Microbiological picture of onychopathy in psoriatic patients." Medical alphabet, no. 6 (June 16, 2020): 63–65. http://dx.doi.org/10.33667/2078-5631-2020-6-63-65.

Full text

Abstract:

Objective. To evaluate the incidence of onychomycosis and bacterial contamination of onychopathy in patients with psoriasis.Material and methods. The study included 86 patients with skin psoriasis and abnormal nail plates or isolated nail psoriasis. Patients nail plates examined in laboratory using direct microscopy with 20 % KOH, mycological culture Sabourauds Dextrose Agar with chloramphenicol and сycloheximide, and bacteriological culture with indetification using the MALDI-TOF mass spectrometer.Results. Out of 86 patients, 27 (31.4 %) had onychomycosis (KOH-positive or KOH-negative with a positive result for dermatophytes in a culture study). Of the 27 patients with onychomycosis, 9 caused by pathogenic fungi, and 18 caused by opportunistic fungi. Of the 54 patients with nail psoriasis, 9 (16.7 %) had onychomycosis, 3 had dermatophytes, and 6 had opportunistic micromycetes. A total of 97 microbiological studies were conducted in 86 patients, in which the following microorganisms were detected: Staphylococcus caprae – 28 strains, Staphylococcus lugdunensis – 26, Staphylococcus epidermidis – 26, Staphylococcus haemolyticus – 15, Staphylococcus pettenkoferi – 13, Staphylococcus simulans – 11, Staphylococcus warneri – 8, Staphylococcus aureus – 5, Staphylococcus piscifermentans – 4, corinebacteria spp. – 3, Staphylococcus hominis – 3, Staphylococcus capitis – 3, Pseudomonas aeruginosa – 3, Staphylococcus pasteuri – 1, Staphylococcus cohnii – 1, Kocuria spp. – 1, Klebsiella pneumonia – 1.Conclusion. In our study, onychomycosis was detected in 31.4 % of patients with psoriasis who have onychodystrophy. In psoriatic onychia, onychomycosis occurred in 16.7 % cases. Pseudomonas nail infection was observed in two patients, one in combination with nail psoriasis.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Microbiological culture'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles