Academic literature on the topic 'Microbiology - Culture media and culture'

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Journal articles on the topic "Microbiology - Culture media and culture"

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Traynor, Peter. "Culture Media SIG." Microbiology Australia 33, no. 4 (2012): 000. http://dx.doi.org/10.1071/ma12903.

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The Culture Media Special Interest Group of the Australian Society for Microbiology was formed in 1991 by a group of interested individuals after an upsurge in interest in the issue of media quality and the appearance that no common standards or consensus existed in this area in Australia. Increased interest, especially amongst medical microbiologists, in what was being done, or should be done, by way of assuring the quality of microbiological media made the issue contentious.
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Condayan, Chris. "Culture media." Nature Reviews Microbiology 6, no. 9 (September 2008): 646. http://dx.doi.org/10.1038/nrmicro1981.

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Merlino, John. "Applications and integration of chromogenic culture media in clinical microbiology." Microbiology Australia 31, no. 3 (2010): 127. http://dx.doi.org/10.1071/ma10127.

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The ability to rapidly detect and identify micro-organisms is of paramount importance for many microbiology laboratories. The application of new classes of enzymatic-based chromogenic compounds has revolutionised traditional culture media, leading to the developments of a new generation of chromogenic media. Many studies have shown that the chromogenic agar has become more than an isolation media when compared to conventional culture media. A newer, faster, more cost-effective means in the presumptive identification or screening of microorganisms which are either genus, or in some cases species-specific, and allows superior differentiation of mixed micro-organisms in cultures. When supplemented with antibiotics or other agents, chromogenic media allows the screening for multidrug resistance and virulence in many micro-organisms and can be easily integrated with other automated and/or molecular-based methods.
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Kenna, Margaret A. "Microbiology of Chronic Suppurative Otitis Media in Children." Annals of Otology, Rhinology & Laryngology 97, no. 2_suppl (March 1988): 9–15. http://dx.doi.org/10.1177/00034894880970s204.

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The aerobic microorganisms present in 57 MEs of 40 children with C Supp OM without cholesteatoma were evaluated. Specimens were taken directly from the ME through a patent perforation or tympanostomy tube and cultured using standard techniques. Twenty-six organisms were identified, with Pseudomonas aeruginosa being the most prevalent (65% of ears) and the only organism in 30% of ears. The initial treatment of all patients was intravenous administration of an antimicrobial selected on the basis of culture and susceptibility reports. This treatment was successful in all but four children, who subsequently required tympanomastoid surgery. These results indicate that the microbiology of C Supp OM is similar to that in patients with cholesteatoma. However, based on the results of culture and susceptibility studies, medical therapy provides a viable alternative to major mastoid surgery in the management of C Supp OM without cholesteatoma.
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Kelly, Devin, Julie Rizzo, Heather Yun, and Dana Blyth. "Microbiology and Clinical Characteristics of Industrial Oil Burns." Open Forum Infectious Diseases 4, suppl_1 (2017): S109. http://dx.doi.org/10.1093/ofid/ofx163.111.

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Abstract Background Injured oil workers are exposed to a broad microbiome in hydraulic fracturing fluids (HFF) and oil wells at the time of injury. This includes Pseudomonas, Stenotrophomonas, Acinetobacter, and rare human pathogens which may be harder to culture. This study evaluates oil-related burn (ORB) microbiology. Methods Patients admitted to the USAISR burn center enrolled in the Epidemiology of Workplace Burns and Injuries in Texas registry from April 2011 to November 2016 were included as cases and controls. Patients hospitalized ≤2 days were excluded. ORB was defined as exposure to HFF (FORB), or non-HFF (NFORB). Controls were patients admitted with industrial burns (non-ORB). Patient demographics and clinical cultures (days 1–15) were obtained through the registry and electronic medical record. Results 149 industrial burns were included, of which 35 (23%) were ORB and 114 (77%) were non-ORB. Of the ORB, 11 (31%) were FORB and 24 (69%) were NFORB. ORB had a median age, TBSA, and Baux score of 31, 25, and 58 compared with non-ORB with 36, 4, and 44, respectively (P < 0.01). Twenty-five patients had positive cultures: 12 (48%) non-ORB and 13 (52%) ORB. Sixty Isolates identified from the ORB population included Flavobacterium, Pseudomonas, and Serratia. FORB accounted for three (25%) of the culture positive ORB. S. marcescens was isolated in 1 FORB (33%) compared with 0 NFORB and non-ORB (P < 0.05). Otherwise, there was no statistical difference in isolates. Median time to first positive culture differed among non-ORB (4 days), FORB (13 days), and NFORB (3.5 days, P = 0.03). Forty-six (31%) patients had cultures obtained during admission: three (7%) FORB, 12 (26%) NFORB, and 31 (67%) non-ORB. Of cultured patients, ORB had a median TBSA and Baux score of 44 and 90 compared with non-ORB with 11 and 47, respectively (P < 0.01). Comparing all cultured patients, ORB had more positive, negative, and total cultures compared with non-ORB with 2 vs. 0, 7 vs. 3, and 10 vs. 3, respectively (P < 0.01). Conclusion Within this cohort, ORB was associated with more severe injuries compared with non-ORB. They had more positive, negative, and total cultures, and recovery of S. marcescens was associated with FORB. Larger studies with non-culture based technology could help further define the microbiology of this uniquely exposed population. Disclosures All authors: No reported disclosures.
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Farfour, Eric, Lucie Limousin, Amandine Henry, Emilie Cardot, Pierre Cahen, Didier Lecointe, Emilie Jolly, Marc Vasse, and Damien Mathonnet. "Optimization of incubation duration of culture media in microbiology." Annales de Biologie Clinique 77, no. 5 (October 2019): 525–31. http://dx.doi.org/10.1684/abc.2019.1474.

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ESTEBAN, M. A., M. ALCALA, A. MARCOS, J. FERNANDEZ-SALGUERO, G. D. GARCIA DE FERNANDO, J. A. ORDOÑEZ, and B. SANZ. "Water activity of culture media used in food microbiology." International Journal of Food Science & Technology 25, no. 4 (June 28, 2007): 464–68. http://dx.doi.org/10.1111/j.1365-2621.1990.tb01105.x.

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Shub, T. A., G. Ya Kivman, V. S. Chudaeva, V. G. Maslennikova, and O. N. Al'bitskaya. "Use ofChlorella hydrolysate in culture media and pharmaceutical microbiology." Pharmaceutical Chemistry Journal 28, no. 7 (July 1994): 513–15. http://dx.doi.org/10.1007/bf02219251.

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Basu, S., A. Pal, and PK Desai. "Quality control of culture media in a microbiology laboratory." Indian Journal of Medical Microbiology 23, no. 3 (2005): 159. http://dx.doi.org/10.4103/0255-0857.16586.

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Sauvage, Justine, Gary H. Wikfors, Xiaoxu Li, Mark Gluis, Nancy Nevejan, Koen Sabbe, and Alyssa Joyce. "Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species." Applied Microbiology and Biotechnology 105, no. 5 (February 12, 2021): 2139–56. http://dx.doi.org/10.1007/s00253-021-11147-8.

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Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect of NAC and two pluronic (F127 and F68) culture media additives upon the culture productivity of six marine microalgal species of relevance to the aquaculture industry (four diatoms-Chaetoceros calcitrans, Chaetoceros muelleri, Skeletonema costatum, and Thalassiosira pseudonana; two haptophytes-Tisochrysis lutea and Pavlova salina). Algal culture performance in response to the addition of NAC and pluronic, singly or combined, is dosage- and species-dependent. Combined NAC and pluronic F127 algal culture media additives resulted in specific growth rate increases of 38%, 16%, and 24% for C. calcitrans, C. muelleri, and P. salina, respectively. Enhanced culture productivity for strains belonging to the genus Chaetoceros was paired with an ~27% increase in stationary-phase cell density. For some of the species examined, culture media enrichments with NAC and pluronic resulted in increased omega-3-fatty acid content of the algal biomass. Larval development (i.e., growth and survival) of the Pacific oyster (Crassostrea gigas) was not changed when fed a mixture of microalgae grown in NAC- and F127-supplemented culture medium. Based upon these results, we propose that culture media enrichment with NAC and pluronic F127 is an effective and easily adopted approach to increase algal productivity and enhance the nutritional quality of marine microalgal strains commonly cultured for live-feed applications in aquaculture. Key points • Single and combined NAC and pluronic F127 culture media supplementation significantly enhanced the productivity of Chaetoceros calcitrans and Chaetoceros muelleri cultures. • Culture media enrichments with NAC and F127 can increase omega-3-fatty acid content of algal biomass. • Microalgae grown in NAC- and pluronic F127-supplemented culture media are suitable for live-feed applications.
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Dissertations / Theses on the topic "Microbiology - Culture media and culture"

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Bateman, Colin Neil. "Investigating gene induction in Listeria monocytogenes during growth in complex media or tissue culture." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/3619/.

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Lopes, Isabella de Cenço [UNESP]. "Produção de conídios do fungo entomopatogênico Metarhizium anisopliae em diferentes condições de cultivo e em biorreator de bandeja." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/139445.

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O objetivo deste trabalho foi de aumentar a produção de conídios de Metarhizium anisopliae através de alterações do substrato e das condições de cultivo e produzi-lo em biorreator de bandeja. Os substratos testados foram arroz tipo 1, quirera de arroz e farelo de arroz, sendo o cultivo realizado em embalagens plásticas contendo 10 g de substrato seco. Inicialmente, foi empregado arroz tipo 1 como substrato, variando-se as formas de umidificação no seu preparo, sendo o cultivo realizado em embalagens plásticas contendo 10g de substrato seco. Determinada a condição adequada de umidificação, os substratos arroz tipo 1 e quirera de arroz foram submetidos a diferentes condições de fotoperíodo: escuto contínuo, claro contínuo e escuro e claro alternados. O farelo de arroz foi adicionado a bagaço de cana-de-açúcar de modo a estruturar fisicamente o meio de cultivo. Os melhores resultados foram obtidos com arroz e quirera de arroz. A primeira alternativa de ampliação de escala foi realizada em embalagens plásticas, utilizando arroz tipo 1 e quirera com 500g de substrato seco. A etapa seguinte da ampliação de escala foi em um biorreator de bandeja com aeração realizada sobre a camada de substrato, sendo os testes realizados com arroz tipo 1, e duas espessuras de camada partículas, 2 e 4 cm. Em todos os ensaios, a resposta observada foi a concentração final de conídios. Foi testada ainda a virulência dos conídios produzidos em relação a lagartas de Diatraea flavipennella nas diferentes condições de produção. De acordo com os resultados apresentados, o farelo de arroz não é uma boa opção de substrato devido a sua pouca praticidade de manipulação e a quirera apresentou resultados satisfatórios nos ensaios, podendo ser considerada uma opção viável para utilização industrial devido ao seu baixo custo. O biorreator de bandeja elaborado apresentou bons resultados de produção de conídios em relação a produção em embalagem plástica de maior capacidade com o substrato arroz tipo 1, a qual, no entanto, apresentou produção de esporos inferior à da embalagem de menor capacidade. Os testes de virulência comprovaram a eficiência dos conídios em todos os ensaios com pequenas variações no tempo de mortalidade.
The work targeted the increase of the production of spores of Metarhizium anisopliae through modifications of the substrate and of the cultivation conditions, and produce such spores in a tray bioreactor. Type 1 rice, broken rice and rice bran were tested as substrate, which were cultivated in plastic bags containing 10 g of dry substrate. Alternatives of humidification were tested with type 1 rice. For the best alternative of humidification, type 1 rice and broken rice were submitted to alternatives of different exposures to light, provided by a fluorescent lamp: continuous dark, continuous light, alternation between dark and light. To the bran was added sugar cane bagasse in order to provide physical structure to the culture medium. The best results were obtained with rice and broken rice and the illumination regime does not influence on the spore production. The first attempt of scaling-up the spore production was the use of plastic bags containing 500 g of dry substrate, either rice or broken rice. The next scaling-up step was in a tray bioreactor aerated flowing air parallel to the top of the cultivation medium, using type 1 rice as substrate in two different thickness of medium, 2 and 4 cm. In every experiments, the response variable was the conidia concentration. The virulence against Diatraea flavipennella caterpillars was also tested for spores produced in the different experimental conditions. The results showed that rice bran is not an efficient alternative due to its difficult manipulation, while broken rice produce conidia concentration similar to type 1 rice and can be considered a cheap alternative for industrial production of spores. The tray bioreactor presented similar results to the large capacity plastic bags, which presented lower conidia concentrations than the small capacity container. The virulence experiments showed high efficiency of the conidia in all tested samples, with little variation in the time of lethality.
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Costa, André Luis da 1982. "Corte normalizado em grafos = um algoritmo aglomerativo para segmentação de imagens de colonias de bactérias= Normalized cut on graphs: an aglomerative algorithm for bacterial colonies image segmentation." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267753.

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Orientador: Marco Antonio Garcia de Carvalho
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Tecnologia
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Resumo: O problema de segmentação de colônias de bactérias em placas de Petri possui algumas características bem distintas daquelas encontradas, por exemplo, em problemas de segmentação de imagens naturais. A principal característica é o alto número de colônias que podem ser encontradas em uma placa. Desta forma, é primordial que o algoritmo de segmentação seja capaz de realizar a segmentação da imagem em um grande número de regiões. Este cenário extremo é ideal para analisar limitações dos algoritmos de segmentação. De fato, neste trabalho foi verificado que o algoritmo de corte normalizado original, que se fundamenta na teoria espectral de grafos, é inadequado para aplicações que exigem que a segmentação seja realizada em um grande número de regiões. Contudo, a utilização do critério de corte normalizado para segmentar imagens de colônias de bactérias ainda é possível graças a um novo algoritmo que está sendo introduzido neste trabalho. O novo algoritmo fundamenta-se no agrupamento hierárquico dos nós do grafo, ao invés de utilizar conceito da teoria espectral. Experimentos mostram também que o biparticionamento de um grafo pelo novo algoritmo apresenta um valor de corte normalizado médio cerca de 40 vezes menor que o biparticionamento pelo algoritmo baseado na teoria espectral
Abstract: The problem of bacteria colonies segmentation in Petri dishes has some very different characteristics from those found, for example, in segmenting natural images. The main feature is the high number of colonies that can be found on a plate. Thus, it is essential that the segmentation algorithm is capable of performing the image segmentation into a huge number of regions. This extreme scenario is ideal for analyzing segmentation algorithms limitations. In fact, this study showed that the original normalized cut algorithm, which is based on the spectral graph theory, is inappropriate for applications that require that the segmentation be performed on a large number of regions. However, the use of normalized cut criteria for segmenting bacteria colonies images is still possible thanks to a new algorithm that is being introduced in this paper. The new algorithm is based on hierarchical clustering of the graph nodes, instead of using the spectral theory concepts. Experiments also show that the bi-partitioning of a graph by the new algorithm has a normalized cut average value about 40 times lesser than the bi-partitioning by the algorithm based on the spectral theory
Mestrado
Tecnologia e Inovação
Mestre em Tecnologia
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Ortmanis, Andris. "The development of a microcomputer controlled variable pathlength turbidimeter /." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65460.

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Elhaneid, Mohamed. "Toxicological assessment of graphene based nanomaterials in cell culture models." Thesis, University of Birmingham, 2019. http://etheses.bham.ac.uk//id/eprint/8881/.

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Graphene oxide (GO) and reduced GO (r-GO) nanomaterials exhibit great potential for several biomedical applications. Of foremost importance is to determine any potential health hazards related in their exposure. In this research, we hypothesised that the different material properties evidenced by GO and r-GO would elicit different biological responses. The first objective of this work was to synthesize Go and r-GO and characterize their physiochemical properties. The second aim was to investigate whether the two-distinct surface chemistries of GO and r-GO influenced their biological effect. The potential toxicity of these nanomaterials was investigated using the normal lung fibroplast cell line MRC-5 and cancerous epithelial lung cell line A549. The cytotoxicity of graphene derivatives was concentration-, time- and cell-dependent and varied according to the material used. Thus, the surface chemistry of graphene plays a critical role in its biocompatibility. Non-cancerous cells had a higher sensitivity to GO cytotoxicity than cancer cells. R-GO was highly biocompatible to MRC-5 cells and for A549 cells had a minimal effect of cell viability. At 37C˚, GO and r-GO were moderately hemolyric at concentration of 125 µg/ml and highly haemolytic at concentration of 300 µg/ml. Exposure of cells to both graphene derivatives led to reactive oxygen species (RO5) generation without genotoxicity. GO, but not r-GO, led to autophagy in both cell lines, possibly inhibiting the PIP3-Akt/mTOR pathway. For both cell lines and at non-lethal concentrations, GO downregulated the expression of glycogen synthase kinase-3 (GSK-3ß). GO was also found to dysregulate both Wnt/b-catenin and Akt cell signalling pathways which are vital for cellular function. The finding relating to cell signalling provide an insight to the safety of GO which is important to its use in cancer therapy.
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Bombardi, Franciele Mendes de Lima. "Sensoriamento ótico da dinâmica do crescimento de colônias de escherichia coli em ambiente hídrico." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2526.

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Este trabalho apresenta um estudo empregando duas técnicas óticas para monitorar o crescimento de culturas de cepas de Escherichia coli em dois meios de cultura líquidos: Espectroscopia de absorção UV-Vis (turbidimetria) e espectroscopia Raman. Na primeira técnica, a turbidez permite avaliar as diferentes fases naturais de crescimento de uma cultura bacteriana (lag, exponencial, estacionária e decaimento) por meio da densidade ótica, medida com um espectrômetro UV-VIS. Na segunda, o espalhamento Raman (medido com um espectrômetro dispersivo), a partir de amostras de água contaminada, fornece não apenas informações sobre as fases de crescimento, mas também abre a possibilidade de identificação bacteriana através da sua impressão digital característica. Mediu-se a dinâmica de duas cepas de E. coli – (nomeadas como H2/11 e H3C2/12) em um caldo líquido nutriente e quatro cepas de E. coli (nomeadas como H2/11, H3C2/12, 109 e 110) em caldo líquido EC, mantidas a 37,0°C ao longo de 24 horas. Alíquotas das amostras foram removidas da cultura em intervalos de tempo regulares para medições espectrais. A análise da turbidez permitiu medir o tempo de geração (isto é, o tempo de duplicação de uma população), que foi maior para cepas crescidas em caldo EC. Os espectros Raman forneceram informações sobre a evolução temporal das bandas a 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associadas com impressões digitais de componentes biológicos específicos. Os dados espectrais foram analisados por Análise de Componentes Principais (PCA). Os resultados obtidos em ambas as técnicas permitiram identificar as fases lag, exponencial e estacionária das cepas estudadas.
This work is a study using two optical techniques to monitor the growth of cultures of Escherichia coli strains in two liquid culture medium: Raman spectroscopy and UV-Vis absorption spectroscopy (turbidimetry). In one hand, turbidity allows evaluating the different phases of growth of a bacterial culture (lag, exponential, stationary and decay) by optical density, measured with an UV-VIS spectrometer. On the other hand, Raman scattering (measured with a dispersive spectrometer) from contaminated water samples not only provides information about the grow phases, but also opens a possible identification of bacterial by its characteristic fingerprint. Two strains of E. coli (named as H2 / 11 and H3C2 / 12) were measured in liquid nutrient broth and four E. coli strains (named H2 / 11, H3C2 / 12, 109 and 110) in EC liquid broth, kept at 37.0 ° C over 24 hours. Aliquots of the samples were removed from the culture at regular time intervals for spectral measurements. The turbidity analysis allowed to measure the generation time, which was higher for strains grown in EC broth. Raman spectra provided information about the time evolution of the bands at 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associated with fingerprints of biological components. Data were analyzed by Principal Component Analysis (PCA). These results of both techniques allowed identifying the phases lag, exponential and stationary of the studied strains.
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Schöndube, Andrea. "Illness, Media, and Culture." Doctoral thesis, Humboldt-Universität zu Berlin, Philosophische Fakultät II, 2011. http://dx.doi.org/10.18452/16327.

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Vergleichsweise wenige Texte in den Printmedien beschäftigen sich mit Allergie als Gegenstand öffentlichen Interesses. Deshalb untersucht die Dissertation die Darstellung von Allergien in Lifestyle-Magazinen im englisch- und amerikanischsprachigen Raum. Die vorliegende Arbeit befasst sich mit der Verbreitung von medizinischem Wissen durch die Medien. Sie zeigt, ob und wie die Medien zu Aufklärung und Information über Krankheiten, deren Relevanz, Diagnostik und Therapie, beitragen. Sie geht dabei besonders auf den Bedeutungsbereich von Wörtern ein, die als Metaphern benutzt werden. Das Fundament der Überlegungen bildet der Essay „Illness as Metaphor“ von Susan Sontag, in dem sie die Darstellung von Krankheiten und die Benutzung von Stereotypen abhandelt und Fragen, die im Zusammenhang mit Krankheit als sozialer und kultureller Angelegenheit stehen, aufwirft. Um den populärwissenschaftlichen Diskurs der analysierten Artikel in dieser Arbeit zu verstehen, wurde die von Jürgen Link entwickelte Diskursanalyse herangezogen, die sich eng an die Diskurstheorie Foucaults anlehnt. Die semiotischen Deutungsansätze werden mit Hilfe der Untersuchungen von Roland Barthes erklärt. Ziel der Arbeit ist es zu zeigen, wie die verschiedenen Diskurse ineinander greifen, welcher Mechanismus sich dahinter verbirgt und wo Ansatzpunkte für eine sachgerechte publizistische Behandlung des Themas liegen. Der Nutzen von Metaphern in der Beschreibung von Krankheiten liegt darin, dass sie kollektive Gefühlslagen ansprechen und den Denkraum des Möglichen erweitern. Dieser Aspekt ist besonders wichtig, da das Wort Allergie zu einer praktischen und populären Metapher für eine Reihe persönlicher, beruflicher oder politischer Antipathien geworden ist. Obwohl allergische Erkrankungen in der modernen Welt immer häufiger auftreten, räumen ihnen die Medien noch nicht den Platz ein, den sie -- auch ausgehend von der volkswirtschaftlichen Bedeutung der Erkrankungen -- dringend haben müssten.
Only a few print media focus on allergies as a matter of public interest. For this reason the dissertation analyzes the presentation of allergies in English and American lifestyle magazines. This thesis examines the propagation of medical knowledge via the media. It shows if and how the media contribute to health education and information about illness, its relevance, diagnostic investigation as well as therapy. The main focus is on those words which are used as metaphors. They represent an important subject of Susan Sontag''s essay „Illness as Metaphor“ which demonstrates the presentation of illness, the use of stereotypes and thus raises issues about illness being a social and cultural matter of interest. To understand the popular scientific discourse of this dissertation Jürgen Links'' discourse analysis is being used which follows closely Foucault''s theory. The semiotic interpretation is supported by the theories developped by Roland Barthes. This dissertation aims to show how the different discourses intertwine, to bring to fore the underlying mechanisms as well as an appropiate journalistic approach. The benefit of using metaphors when describing illness is that the collective state of mind is addressed and thus the range of thinking will be broadened. This aspect is especially important because the word allergy became a convenient and popular metaphor for a number of personal, professional or political aversions. Although allergies are dramatically on the raise in the modern world, their significance is not recognized yet by the media in their complexity as it should be the case, especially against the backdrop of the economic relevance of that illness
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Saied, Kaj. "News Media in War Culture." Thesis, Karlstad University, Karlstad University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-1476.

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Fear has found its latest instrument in the news media. The discourse of fear in news presentations produces gasping meanings, which we can compellingly indulge in. Fear not just being entertaining, but one of the ways in which we relate to reality, is used as a protection mechanism of our status quo. The purpose of this thesis is to examine the extent to which Fox News tends to use, and further reproduce, the fear discourse to form identities and meaning. The method utilized in this thesis is frame analysis, which is a form of discourse analysis. The primary results indicate that Fox News undeniably uses the fear discourse, for entertainment and the proliferation of the status quo - meaning system. In addition, Fox News applies fear blatantly in the news presentations, as acts of courage and virtuous loyalty to reporting.

Key words: Fear, Frame analysis, Meaning, News media, Infotainment.

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Orth, Zaida. "Rape culture and social media: Exploring how social media influences students’ opinions and perceptions of rape culture." University of the Western Cape, 2018. http://hdl.handle.net/11394/6872.

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Magister Artium (Psychology) - MA(Psych)
In April 2016 students from South African universities launched the #Endrapeculture movement to protest their universities’ institutional policies towards sexual assault on campus, which was seen as perpetuating a rape culture. Through the use of social media, students from across South Africa were able to provide instrumental information and mobilise support for the protests. This thesis focused on exploring the rape culture discourse that emerged from the online debates following the #Endrapeculture protests, as well as the potential of social media as an accessible and affordable pedagogical tool to address rape culture on campus. An exploratory qualitative design was used and this was framed within a postmodern feminist framework. To address the aims of the study two methods of data collection were utilised. All ethics principles were adhered to for both forms of data collection. Firstly, natural observation of comment threads of Facebook relating to the April 2016 #Endrapeculture protests was conducted. A total of 590 comments from 8 Facebook posts were collected and analysed using qualitative content analysis. The findings indicate that rape culture discourses were prominent within these comment threads with Perpetuating Victim-blaming emerging as the most significant theme followed by Rape or Rape Culture, Patriarchy, Race and Culture, Sexualisation and Bodily Autonomy, Trivialising Rape Culture and Role of Universities and Law Enforcement. The second part of the data collection involved conducting online, asynchronous focus groups using the Facebook secret chat group application. Participants for the SFFG were recruited on Facebook through a process of snowball sampling. A total of three SFFG were conducted with 16 participants. Thematic decomposition analysis was used to analyse the data. The findings revealed three main themes namely; Defining Rape Culture, Learning about Rape Culture and The Role of Social Media. Based on the observations from the comment threads and the findings from the SFFGs, it is argued that social media can be used as a pedagogical tool to address rape culture on campus in two ways. Firstly, it is beneficial on a macro level by using social media platforms to provide instrumental information about rape culture. Secondly, it can be utilised on a micro level by using applications like the SFFG to provide a safe space where students can engage in small-scale interactive discussions.
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Brodkin, Kathryn Rhea. "Chondrocyte behavior in monolayer culture : the effects of protein substrates and culture media." Thesis, Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/20216.

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Books on the topic "Microbiology - Culture media and culture"

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W, Snyder James, ed. Handbook of media for clinical microbiology. 2nd ed. Boca Raton: CRC Press, 2006.

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W, Snyder James, ed. Handbook of media for clinical microbiology. Boca Raton: CRC Press, 1995.

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Digestive Ferment Company. Difco Laboratories, Detroit. Difco manual: Dehydrated culture media and reagents for microbiology. Detroit: DIFCO Laboratories, 1994.

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Atlas, Ronald M. Handbook of media for environmental microbiology. Boca Raton: CRC Press, 1995.

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Handbook of media for environmental microbiology. 2nd ed. Boca Raton: Taylor & Francis, 2005.

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C, Parks Lawrence, ed. Handbook of microbiological media. Boca Raton: CRC Press, 1993.

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C, Parks Lawrence, ed. Handbook of microbiological media. 2nd ed. Boca Raton: CRC Press, 1997.

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1932-, Jennings D. H., ed. Microbial culture. Oxford, UK: Bios Scientific Publishers, 1995.

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Corry, Janet E. L., Gordon D. W. Curtis, and R. M. Baird, eds. Handbook of Culture Media for Food and Water Microbiology. Cambridge: Royal Society of Chemistry, 2011. http://dx.doi.org/10.1039/9781847551450.

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Atlas, Ronald M. Handbook of microbiological media. 4th ed. Boca Raton: Taylor & Francis, 2010.

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Book chapters on the topic "Microbiology - Culture media and culture"

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Kumar, Surinder. "Culture Media." In Textbook of Microbiology, 55. Jaypee Brothers Medical Publishers (P) Ltd., 2012. http://dx.doi.org/10.5005/jp/books/11668_6.

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SenGupta, Manideepa, and Mallika Sengupta. "Culture media." In Practicals in Microbiology, 23. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12695_4.

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Kumar, Surinder. "Culture Media." In Essentials of Microbiology, 37. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12697_7.

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Sandle, Tim. "Microbiological culture media." In Pharmaceutical Microbiology, 47–61. Elsevier, 2016. http://dx.doi.org/10.1016/b978-0-08-100022-9.00005-0.

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Kumar, Surinder. "Culture Media." In Practical Microbiology for MBBS Students, 15. Jaypee Brothers Medical Publishers (P) Ltd., 2018. http://dx.doi.org/10.5005/jp/books/14195_5.

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Selvaraj, Logeswari. "Culture Media." In Textbook of Microbiology for Paramedicals, 24. Jaypee Brothers Medical Publishers (P) Ltd., 2008. http://dx.doi.org/10.5005/jp/books/11041_5.

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Sastry, Apurba, Sandhya K, and Anand Janagond. "Culture Media and Culture Methods." In Essentials of Medical Microbiology, 43. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12637_5.

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Rao, RR. "Culture Media." In Textbook of Microbiology for Nursing Students, 26. Jaypee Brothers Medical Publishers (P) Ltd., 2014. http://dx.doi.org/10.5005/jp/books/12375_6.

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Anthikad, Jacob, and P. Sumanaswini. "Culture Media." In Medical Microbiology for Nurses (Including Parasitology), 21. Jaypee Brothers Medical Publishers (P) Ltd., 2013. http://dx.doi.org/10.5005/jp/books/11930_6.

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Rao, RR. "Culture Media." In Textbook of Microbiology for Nursing Students, 28. Jaypee Brothers Medical Publishers (P) Ltd., 2005. http://dx.doi.org/10.5005/jp/books/10922_6.

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Conference papers on the topic "Microbiology - Culture media and culture"

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Valle-Algarra, F. M., Eva M. Mateo, M. A. García-Esparza, R. Mateo, J. V. Gimeno-Adelantado, and M. Jiménez. "Production of patulin by Penicillium expansum in different culture media including bee pollen media." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0092.

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Ren, Nanqi, Yongfeng Li, Maryam Zadsar, Lijie Hu, and Jianzheng Li. "Biological Hydrogen Production In China: Past, Present and Future." In ASME 2005 International Solar Energy Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/isec2005-76101.

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As a new clean energy source and important material, the use and demand of hydrogen are increasing-rapidly. So that bio-hydrogen producing technology moves toward cutting down the operation costs in recent years. Biohydrogen production capacity improvement and cost reduction are two key points for industrialization of the process. Biohydrogen production has been studied in China for over 20 years in both photosynthetic hydrogen production and fermentative processes fields. The anaerobic process of fermentative hydrogen production has been developing in China since 1990s. The isolation and identification of high efficient bio-hydrogen production anaerobic bacteria is an important foundation of fermentative bio-hydrogen production process by anaerobic digestion of organic wastewater. The paper focuses on: (1) Fermentative biohydrogen production system, (2) Laboratory experiments and pilot scale tests for continued hydrogen production, (3) Fermentation types and their engineering control, (4) isolation, culture media and characterization of anaerobes, (5) Applications of pure bacteria, (6) Fundamental researches including ecology, genetics and improvements, (7) Development of two-phase anaerobic process of H2-producing and methanogenic phases as, and (8) the integrated processes with bioengineering and wastewater treatments. Recently, the first pilot factory has been costructedin Harbin, China by hydrogen production rate of more than 1200m3/d which located in northeast of China. In photosynthetic hydrogen production filed, study is focused on the fundamentals, engineering application and microbiology. Detailed discussion comes later.
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Oehri, Caroline, and Stephanie Teufel. "Social media security culture." In 2012 Information Security for South Africa (ISSA). IEEE, 2012. http://dx.doi.org/10.1109/issa.2012.6320436.

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Arai, Kazuhiro. "Vernacular Religion and SNS-Media Practices." In 2013 International Conference on Culture and Computing (Culture Computing). IEEE, 2013. http://dx.doi.org/10.1109/culturecomputing.2013.80.

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Nakatsu, Ryohei, and Chamari Edirisinghe. "Artistic Communication Using Digital Media." In 2011 Second International Conference on Culture and Computing (Culture Computing). IEEE, 2011. http://dx.doi.org/10.1109/culture-computing.2011.43.

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Hu, Jun, Feng Wang, Mathias Funk, Joep Frens, Yu Zhang, Thom Van Boheemen, Chenxi Zhang, Qi Yuan, Hongrui Qu, and Matthias Rauterberg. "Participatory Public Media Arts for Social Creativity." In 2013 International Conference on Culture and Computing (Culture Computing). IEEE, 2013. http://dx.doi.org/10.1109/culturecomputing.2013.54.

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Wiggins, Bradley. "NAVIGATING DIGITAL CULTURE: REMIX CULTURE, VIRAL MEDIA, AND INTERNET MEMES." In International Technology, Education and Development Conference. IATED, 2017. http://dx.doi.org/10.21125/inted.2017.0221.

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Edirisinghe, Chamari, Ryohei Nakatsu, Johannes Widodo, and Adrian David Cheok. "Conceptualizing Third Space in Networked Social Media." In 2011 Second International Conference on Culture and Computing (Culture Computing). IEEE, 2011. http://dx.doi.org/10.1109/culture-computing.2011.31.

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Tosa, Naoko, Ryohei Nakatsu, Pang Yunian, and Liang Zhao. "Creation of Media Art Utilizing Fluid Dynamics." In 2017 International Conference on Culture and Computing (Culture and Computing). IEEE, 2017. http://dx.doi.org/10.1109/culture.and.computing.2017.30.

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Nakatsu, Ryohei, and Chamari Edirisinghe. "Changing Behavior Patterns of New Media Users: A Case Study in Singapore." In 2013 International Conference on Culture and Computing (Culture Computing). IEEE, 2013. http://dx.doi.org/10.1109/culturecomputing.2013.45.

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Reports on the topic "Microbiology - Culture media and culture"

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Zook, Justin M., Robert MacCuspie, John T. Elliott Jr., and Elijah J. Petersen. Reliable preparation of nanoparticle agglomerates of different sizes in cell culture media. National Institute of Standards and Technology, June 2015. http://dx.doi.org/10.6028/nist.sp.1200-14.

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Martínez Sanz, R., O. Islas Carmona, M. Redondo García, and E. Campos Domínguez. Communication professorship: access, consumption and media culture. A comparative study of Spain and Mexico. Revista Latina de Comunicación Social, April 2016. http://dx.doi.org/10.4185/rlcs-2016-1099en.

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Holmes, Aimee E., Landon H. Sego, Bobbie-Jo M. Webb-Robertson, Helen W. Kreuzer, Richard M. Anderson, Stephen D. Unwin, Mark R. Weimar, Mark F. Tardiff, and Courtney D. Corley. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms. Office of Scientific and Technical Information (OSTI), February 2013. http://dx.doi.org/10.2172/1077996.

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Melnyk, Iurii. JUSTIFICATION OF OCCUPATION IN GERMAN (1938) AND RUSSIAN (2014) MEDIA: SUBSTITUTION OF AGGRESSOR AND VICTIM. Ivan Franko National University of Lviv, March 2021. http://dx.doi.org/10.30970/vjo.2021.50.11101.

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The article is dedicated to the examination and comparison of the justification of occupation of a neighboring country in the German (1938) and Russian (2014) media. The objective of the study is to reveal the mechanics of the application of the classical manipulative method of substituting of aggressor and victim on the material of German and Russian propaganda in 1938 and in 2014 respectively. According to the results of the study, clear parallels between the two information strategies can be traced at the level of the condemnation of internal aggression against a national minority loyal to Berlin / Moscow and its political representative (the Sudeten Germans – the pro-Russian Ukrainians, as well as the security forces of the Yanukovych regime); the reflections on dangers that Czechoslovakia / Ukraine poses to itself and to its neighbors; condemnation of the violation of the cultural rights of the minority that the occupier intends to protect (German language and culture – Russian language and culture); the historical parallels designed to deepen the modern conflict, to show it as a long-standing and a natural one (“Hussites” – “Banderites”). In the manipulative strategy of both media, the main focus is not on factual fabrication, but on the bias selection of facts, due to which the reader should have an unambiguous understanding of who is the permanent aggressor in the conflict (Czechoslovakia, Czechs – Ukraine, Ukrainians), and who is the permanent victim (Germans – Russians, Russian speakers). The substitution of victim and aggressor in the media in both cases became one of the most important manipulative strategies designed to justify the German occupation of part of Czechoslovakia and the Russian occupation of part of Ukraine.
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Pavlyuk, Ihor. MEDIACULTURE AS A NECESSARY FACTOR OF THE CONSERVATION, DEVELOPMENT AND TRANSFORMATION OF ETHNIC AND NATIONAL IDENTITY. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11071.

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The article deals with the mental-existential relationship between ethnoculture, national identity and media culture as a necessary factor for their preservation, transformation, on the example of national original algorithms, matrix models, taking into account global tendencies and Ukrainian archetypal-specific features in Ukraine. the media actively serve the domestic oligarchs in their information-virtual and real wars among themselves and the same expansive alien humanitarian acts by curtailing ethno-cultural programs-projects on national radio, on television, in the press, or offering the recipient instead of a pop pointer, without even communicating to the audience the information stipulated in the media laws − information support-protection-development of ethno-culture national product in the domestic and foreign/diaspora mass media, the support of ethnoculture by NGOs and the state institutions themselves. In the context of the study of the cultural national socio-humanitarian space, the article diagnoses and predicts the model of creating and preserving in it the dynamic equilibrium of the ethno-cultural space, in which the nation must remember the struggle for access to information and its primary sources both as an individual and the state as a whole, culture the transfer of information, which in the process of globalization is becoming a paramount commodity, an egregore, and in the post-traumatic, interrupted-compensatory cultural-information space close rehabilitation mechanisms for national identity to become a real factor in strengthening the state − and vice versa in the context of adequate laws («Law about press and other mass media», Law «About printed media (press) in Ukraine», Law «About Information», «Law about Languages», etc.) and their actual effect in creating motivational mechanisms for preserving/protecting the Ukrainian language, as one of the main identifiers of national identity, information support for its expansion as labels cultural and geostrategic areas.
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Hotsur, Oksana. SOCIAL NETWORKS AND BLOGS AS TOOLS PR-CAMPAIGN IMPLEMENTATIONS. Ivan Franko National University of Lviv, March 2021. http://dx.doi.org/10.30970/vjo.2021.50.11110.

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The article deals with the ways in which social networks and the blogosphere influence the formation and implementation of a PR campaign. Examples from the political sphere (election campaigns, initiatives), business (TV brands, traditional and online media) have revealed the opportunities that Facebook, Telegram, Twitter, YouTube and blogs promote in promoting advertising, ideas, campaigns, thoughts, or products. Author blogs created on special websites or online media may not be as much of a tool in PR as an additional tool on social media. It is noted that choosing a blog as the main tool of PR campaign has both positive and negative points. Social networks intervene in the sphere of human life, become a means of communication, promotion, branding. The effectiveness of social networks has been evidenced by such historically significant events as Brexit, the Arab Spring, and the Revolution of Dignity. Special attention was paid to the 2019 presidential election. Based on the analysis of individual PR campaigns, the reasons for successful and unsuccessful campaigns from the point of view of network communication, which provide unlimited multimedia and interactive tools for PR, are highlighted. In fact, these concepts significantly affect the effectiveness of the implementation of PR-campaign, its final effectiveness, which is determined by the achievement of goals. Attention is drawn to the culture of communication during the PR campaign, as well as the concepts of “trolls”, “trolling”, “bots”, “botoin industry”. The social communication component of these concepts is unconditional. Choosing a blog as the main tool of a marketing campaign has both positive and negative aspects. Only a person with great creative potential can run and create a blog. In addition, it takes a long time. In fact, these two points are losing compared to other internet marketing tools. Further research is interesting in two respects. First, a comparison of the dynamics of the effectiveness of PR-campaign tools in Ukraine in 2020 and in the past, in particular, at the dawn of state independence. Secondly, to investigate how/or the concept of PR-campaigns in social networks and blogs is constantly changing.
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Halych, Valentyna. SERHII YEFREMOV’S COOPERATION WITH THE WESTERN UKRAINIAN PRESS: MEMORIAL RECEPTION. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11055.

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The subject of the study is the cooperation of S. Efremov with Western Ukrainian periodicals as a page in the history of Ukrainian journalism which covers the relationship of journalists and scientists of Eastern and Western Ukraine at the turn of the XIX-XX centuries. Research methods (biographical, historical, comparative, axiological, statistical, discursive) develop the comprehensive disclosure of the article. As a result of scientific research, the origins of Ukrainocentrism in the personality of S. Efremov were clarified; his person as a public figure, journalist, publisher, literary critic is multifaceted; taking into account the specifics of the memoir genre and with the involvement of the historical context, the turning points in the destiny of the author of memoirs are interpreted, revealing cooperation with Western Ukrainian magazines and newspapers. The publications ‘Zoria’, ‘Narod’, ‘Pravda’, ‘Bukovyna’, ‘Dzvinok’, are secretly got into sub-Russian Ukraine, became for S. Efremov a spiritual basis in understanding the specifics of the national (Ukrainian) mass media, ideas of education in culture of Ukraine at the end of XIX century, its territorial integrity, and state independence. Memoirs of S. Efremov on cooperation with the iconic Galician journals ‘Notes of the Scientific Society after the name Shevchenko’ and ‘Literary-Scientific Bulletin’, testify to an important stage in the formation of the author’s worldview, the expansion of the genre boundaries of his journalism, active development as a literary critic. S. Yefremov collaborated most fruitfully and for a long time with the Literary-Scientific Bulletin, and he was impressed by the democratic position of this publication. The author’s comments reveal a long-running controversy over the publication of a review of the new edition of Kobzar and thematically related discussions around his other literary criticism, in which the talent of the demanding critic was forged. S. Efremov steadfastly defended the main principles of literary criticism: objectivity and freedom of author’s thought. The names of the allies of the Ukrainian idea L. Skochkovskyi, O. Lototskyi, O. Konyskyi, P. Zhytskyi, M. Hrushevskyi in S. Efremov’s memoirs unfold in multifaceted portrait descriptions and function as historical and cultural facts that document the pages of the author’s biography, record his activities in space and time. The results of the study give grounds to characterize S. Efremov as the first professional Ukrainian-speaking journalist.
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Electronic sports media and its impact on the development of sports culture among college students. Aissa Elhadi, Kamel Raach, Kamal Rouibah, June 2018. http://dx.doi.org/10.14526/02_2018_314.

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