Dissertations / Theses: 'Microbiology - Culture media and culture'

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Bateman, Colin Neil. "Investigating gene induction in Listeria monocytogenes during growth in complex media or tissue culture." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/3619/.

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Lopes, Isabella de Cenço [UNESP]. "Produção de conídios do fungo entomopatogênico Metarhizium anisopliae em diferentes condições de cultivo e em biorreator de bandeja." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/139445.

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O objetivo deste trabalho foi de aumentar a produção de conídios de Metarhizium anisopliae através de alterações do substrato e das condições de cultivo e produzi-lo em biorreator de bandeja. Os substratos testados foram arroz tipo 1, quirera de arroz e farelo de arroz, sendo o cultivo realizado em embalagens plásticas contendo 10 g de substrato seco. Inicialmente, foi empregado arroz tipo 1 como substrato, variando-se as formas de umidificação no seu preparo, sendo o cultivo realizado em embalagens plásticas contendo 10g de substrato seco. Determinada a condição adequada de umidificação, os substratos arroz tipo 1 e quirera de arroz foram submetidos a diferentes condições de fotoperíodo: escuto contínuo, claro contínuo e escuro e claro alternados. O farelo de arroz foi adicionado a bagaço de cana-de-açúcar de modo a estruturar fisicamente o meio de cultivo. Os melhores resultados foram obtidos com arroz e quirera de arroz. A primeira alternativa de ampliação de escala foi realizada em embalagens plásticas, utilizando arroz tipo 1 e quirera com 500g de substrato seco. A etapa seguinte da ampliação de escala foi em um biorreator de bandeja com aeração realizada sobre a camada de substrato, sendo os testes realizados com arroz tipo 1, e duas espessuras de camada partículas, 2 e 4 cm. Em todos os ensaios, a resposta observada foi a concentração final de conídios. Foi testada ainda a virulência dos conídios produzidos em relação a lagartas de Diatraea flavipennella nas diferentes condições de produção. De acordo com os resultados apresentados, o farelo de arroz não é uma boa opção de substrato devido a sua pouca praticidade de manipulação e a quirera apresentou resultados satisfatórios nos ensaios, podendo ser considerada uma opção viável para utilização industrial devido ao seu baixo custo. O biorreator de bandeja elaborado apresentou bons resultados de produção de conídios em relação a produção em embalagem plástica de maior capacidade com o substrato arroz tipo 1, a qual, no entanto, apresentou produção de esporos inferior à da embalagem de menor capacidade. Os testes de virulência comprovaram a eficiência dos conídios em todos os ensaios com pequenas variações no tempo de mortalidade.
The work targeted the increase of the production of spores of Metarhizium anisopliae through modifications of the substrate and of the cultivation conditions, and produce such spores in a tray bioreactor. Type 1 rice, broken rice and rice bran were tested as substrate, which were cultivated in plastic bags containing 10 g of dry substrate. Alternatives of humidification were tested with type 1 rice. For the best alternative of humidification, type 1 rice and broken rice were submitted to alternatives of different exposures to light, provided by a fluorescent lamp: continuous dark, continuous light, alternation between dark and light. To the bran was added sugar cane bagasse in order to provide physical structure to the culture medium. The best results were obtained with rice and broken rice and the illumination regime does not influence on the spore production. The first attempt of scaling-up the spore production was the use of plastic bags containing 500 g of dry substrate, either rice or broken rice. The next scaling-up step was in a tray bioreactor aerated flowing air parallel to the top of the cultivation medium, using type 1 rice as substrate in two different thickness of medium, 2 and 4 cm. In every experiments, the response variable was the conidia concentration. The virulence against Diatraea flavipennella caterpillars was also tested for spores produced in the different experimental conditions. The results showed that rice bran is not an efficient alternative due to its difficult manipulation, while broken rice produce conidia concentration similar to type 1 rice and can be considered a cheap alternative for industrial production of spores. The tray bioreactor presented similar results to the large capacity plastic bags, which presented lower conidia concentrations than the small capacity container. The virulence experiments showed high efficiency of the conidia in all tested samples, with little variation in the time of lethality.

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Costa, André Luis da 1982. "Corte normalizado em grafos = um algoritmo aglomerativo para segmentação de imagens de colonias de bactérias= Normalized cut on graphs: an aglomerative algorithm for bacterial colonies image segmentation." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267753.

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Orientador: Marco Antonio Garcia de Carvalho
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Tecnologia
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Resumo: O problema de segmentação de colônias de bactérias em placas de Petri possui algumas características bem distintas daquelas encontradas, por exemplo, em problemas de segmentação de imagens naturais. A principal característica é o alto número de colônias que podem ser encontradas em uma placa. Desta forma, é primordial que o algoritmo de segmentação seja capaz de realizar a segmentação da imagem em um grande número de regiões. Este cenário extremo é ideal para analisar limitações dos algoritmos de segmentação. De fato, neste trabalho foi verificado que o algoritmo de corte normalizado original, que se fundamenta na teoria espectral de grafos, é inadequado para aplicações que exigem que a segmentação seja realizada em um grande número de regiões. Contudo, a utilização do critério de corte normalizado para segmentar imagens de colônias de bactérias ainda é possível graças a um novo algoritmo que está sendo introduzido neste trabalho. O novo algoritmo fundamenta-se no agrupamento hierárquico dos nós do grafo, ao invés de utilizar conceito da teoria espectral. Experimentos mostram também que o biparticionamento de um grafo pelo novo algoritmo apresenta um valor de corte normalizado médio cerca de 40 vezes menor que o biparticionamento pelo algoritmo baseado na teoria espectral
Abstract: The problem of bacteria colonies segmentation in Petri dishes has some very different characteristics from those found, for example, in segmenting natural images. The main feature is the high number of colonies that can be found on a plate. Thus, it is essential that the segmentation algorithm is capable of performing the image segmentation into a huge number of regions. This extreme scenario is ideal for analyzing segmentation algorithms limitations. In fact, this study showed that the original normalized cut algorithm, which is based on the spectral graph theory, is inappropriate for applications that require that the segmentation be performed on a large number of regions. However, the use of normalized cut criteria for segmenting bacteria colonies images is still possible thanks to a new algorithm that is being introduced in this paper. The new algorithm is based on hierarchical clustering of the graph nodes, instead of using the spectral theory concepts. Experiments also show that the bi-partitioning of a graph by the new algorithm has a normalized cut average value about 40 times lesser than the bi-partitioning by the algorithm based on the spectral theory
Mestrado
Tecnologia e Inovação
Mestre em Tecnologia

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Ortmanis, Andris. "The development of a microcomputer controlled variable pathlength turbidimeter /." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65460.

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Elhaneid, Mohamed. "Toxicological assessment of graphene based nanomaterials in cell culture models." Thesis, University of Birmingham, 2019. http://etheses.bham.ac.uk//id/eprint/8881/.

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Graphene oxide (GO) and reduced GO (r-GO) nanomaterials exhibit great potential for several biomedical applications. Of foremost importance is to determine any potential health hazards related in their exposure. In this research, we hypothesised that the different material properties evidenced by GO and r-GO would elicit different biological responses. The first objective of this work was to synthesize Go and r-GO and characterize their physiochemical properties. The second aim was to investigate whether the two-distinct surface chemistries of GO and r-GO influenced their biological effect. The potential toxicity of these nanomaterials was investigated using the normal lung fibroplast cell line MRC-5 and cancerous epithelial lung cell line A549. The cytotoxicity of graphene derivatives was concentration-, time- and cell-dependent and varied according to the material used. Thus, the surface chemistry of graphene plays a critical role in its biocompatibility. Non-cancerous cells had a higher sensitivity to GO cytotoxicity than cancer cells. R-GO was highly biocompatible to MRC-5 cells and for A549 cells had a minimal effect of cell viability. At 37C˚, GO and r-GO were moderately hemolyric at concentration of 125 µg/ml and highly haemolytic at concentration of 300 µg/ml. Exposure of cells to both graphene derivatives led to reactive oxygen species (RO5) generation without genotoxicity. GO, but not r-GO, led to autophagy in both cell lines, possibly inhibiting the PIP3-Akt/mTOR pathway. For both cell lines and at non-lethal concentrations, GO downregulated the expression of glycogen synthase kinase-3 (GSK-3ß). GO was also found to dysregulate both Wnt/b-catenin and Akt cell signalling pathways which are vital for cellular function. The finding relating to cell signalling provide an insight to the safety of GO which is important to its use in cancer therapy.

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Bombardi, Franciele Mendes de Lima. "Sensoriamento ótico da dinâmica do crescimento de colônias de escherichia coli em ambiente hídrico." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2526.

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Este trabalho apresenta um estudo empregando duas técnicas óticas para monitorar o crescimento de culturas de cepas de Escherichia coli em dois meios de cultura líquidos: Espectroscopia de absorção UV-Vis (turbidimetria) e espectroscopia Raman. Na primeira técnica, a turbidez permite avaliar as diferentes fases naturais de crescimento de uma cultura bacteriana (lag, exponencial, estacionária e decaimento) por meio da densidade ótica, medida com um espectrômetro UV-VIS. Na segunda, o espalhamento Raman (medido com um espectrômetro dispersivo), a partir de amostras de água contaminada, fornece não apenas informações sobre as fases de crescimento, mas também abre a possibilidade de identificação bacteriana através da sua impressão digital característica. Mediu-se a dinâmica de duas cepas de E. coli – (nomeadas como H2/11 e H3C2/12) em um caldo líquido nutriente e quatro cepas de E. coli (nomeadas como H2/11, H3C2/12, 109 e 110) em caldo líquido EC, mantidas a 37,0°C ao longo de 24 horas. Alíquotas das amostras foram removidas da cultura em intervalos de tempo regulares para medições espectrais. A análise da turbidez permitiu medir o tempo de geração (isto é, o tempo de duplicação de uma população), que foi maior para cepas crescidas em caldo EC. Os espectros Raman forneceram informações sobre a evolução temporal das bandas a 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associadas com impressões digitais de componentes biológicos específicos. Os dados espectrais foram analisados por Análise de Componentes Principais (PCA). Os resultados obtidos em ambas as técnicas permitiram identificar as fases lag, exponencial e estacionária das cepas estudadas.
This work is a study using two optical techniques to monitor the growth of cultures of Escherichia coli strains in two liquid culture medium: Raman spectroscopy and UV-Vis absorption spectroscopy (turbidimetry). In one hand, turbidity allows evaluating the different phases of growth of a bacterial culture (lag, exponential, stationary and decay) by optical density, measured with an UV-VIS spectrometer. On the other hand, Raman scattering (measured with a dispersive spectrometer) from contaminated water samples not only provides information about the grow phases, but also opens a possible identification of bacterial by its characteristic fingerprint. Two strains of E. coli (named as H2 / 11 and H3C2 / 12) were measured in liquid nutrient broth and four E. coli strains (named H2 / 11, H3C2 / 12, 109 and 110) in EC liquid broth, kept at 37.0 ° C over 24 hours. Aliquots of the samples were removed from the culture at regular time intervals for spectral measurements. The turbidity analysis allowed to measure the generation time, which was higher for strains grown in EC broth. Raman spectra provided information about the time evolution of the bands at 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associated with fingerprints of biological components. Data were analyzed by Principal Component Analysis (PCA). These results of both techniques allowed identifying the phases lag, exponential and stationary of the studied strains.

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Schöndube, Andrea. "Illness, Media, and Culture." Doctoral thesis, Humboldt-Universität zu Berlin, Philosophische Fakultät II, 2011. http://dx.doi.org/10.18452/16327.

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Vergleichsweise wenige Texte in den Printmedien beschäftigen sich mit Allergie als Gegenstand öffentlichen Interesses. Deshalb untersucht die Dissertation die Darstellung von Allergien in Lifestyle-Magazinen im englisch- und amerikanischsprachigen Raum. Die vorliegende Arbeit befasst sich mit der Verbreitung von medizinischem Wissen durch die Medien. Sie zeigt, ob und wie die Medien zu Aufklärung und Information über Krankheiten, deren Relevanz, Diagnostik und Therapie, beitragen. Sie geht dabei besonders auf den Bedeutungsbereich von Wörtern ein, die als Metaphern benutzt werden. Das Fundament der Überlegungen bildet der Essay „Illness as Metaphor“ von Susan Sontag, in dem sie die Darstellung von Krankheiten und die Benutzung von Stereotypen abhandelt und Fragen, die im Zusammenhang mit Krankheit als sozialer und kultureller Angelegenheit stehen, aufwirft. Um den populärwissenschaftlichen Diskurs der analysierten Artikel in dieser Arbeit zu verstehen, wurde die von Jürgen Link entwickelte Diskursanalyse herangezogen, die sich eng an die Diskurstheorie Foucaults anlehnt. Die semiotischen Deutungsansätze werden mit Hilfe der Untersuchungen von Roland Barthes erklärt. Ziel der Arbeit ist es zu zeigen, wie die verschiedenen Diskurse ineinander greifen, welcher Mechanismus sich dahinter verbirgt und wo Ansatzpunkte für eine sachgerechte publizistische Behandlung des Themas liegen. Der Nutzen von Metaphern in der Beschreibung von Krankheiten liegt darin, dass sie kollektive Gefühlslagen ansprechen und den Denkraum des Möglichen erweitern. Dieser Aspekt ist besonders wichtig, da das Wort Allergie zu einer praktischen und populären Metapher für eine Reihe persönlicher, beruflicher oder politischer Antipathien geworden ist. Obwohl allergische Erkrankungen in der modernen Welt immer häufiger auftreten, räumen ihnen die Medien noch nicht den Platz ein, den sie -- auch ausgehend von der volkswirtschaftlichen Bedeutung der Erkrankungen -- dringend haben müssten.
Only a few print media focus on allergies as a matter of public interest. For this reason the dissertation analyzes the presentation of allergies in English and American lifestyle magazines. This thesis examines the propagation of medical knowledge via the media. It shows if and how the media contribute to health education and information about illness, its relevance, diagnostic investigation as well as therapy. The main focus is on those words which are used as metaphors. They represent an important subject of Susan Sontag''s essay „Illness as Metaphor“ which demonstrates the presentation of illness, the use of stereotypes and thus raises issues about illness being a social and cultural matter of interest. To understand the popular scientific discourse of this dissertation Jürgen Links'' discourse analysis is being used which follows closely Foucault''s theory. The semiotic interpretation is supported by the theories developped by Roland Barthes. This dissertation aims to show how the different discourses intertwine, to bring to fore the underlying mechanisms as well as an appropiate journalistic approach. The benefit of using metaphors when describing illness is that the collective state of mind is addressed and thus the range of thinking will be broadened. This aspect is especially important because the word allergy became a convenient and popular metaphor for a number of personal, professional or political aversions. Although allergies are dramatically on the raise in the modern world, their significance is not recognized yet by the media in their complexity as it should be the case, especially against the backdrop of the economic relevance of that illness

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Saied, Kaj. "News Media in War Culture." Thesis, Karlstad University, Karlstad University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-1476.

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Fear has found its latest instrument in the news media. The discourse of fear in news presentations produces gasping meanings, which we can compellingly indulge in. Fear not just being entertaining, but one of the ways in which we relate to reality, is used as a protection mechanism of our status quo. The purpose of this thesis is to examine the extent to which Fox News tends to use, and further reproduce, the fear discourse to form identities and meaning. The method utilized in this thesis is frame analysis, which is a form of discourse analysis. The primary results indicate that Fox News undeniably uses the fear discourse, for entertainment and the proliferation of the status quo - meaning system. In addition, Fox News applies fear blatantly in the news presentations, as acts of courage and virtuous loyalty to reporting.

Key words: Fear, Frame analysis, Meaning, News media, Infotainment.

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Orth, Zaida. "Rape culture and social media: Exploring how social media influences students’ opinions and perceptions of rape culture." University of the Western Cape, 2018. http://hdl.handle.net/11394/6872.

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Magister Artium (Psychology) - MA(Psych)
In April 2016 students from South African universities launched the #Endrapeculture movement to protest their universities’ institutional policies towards sexual assault on campus, which was seen as perpetuating a rape culture. Through the use of social media, students from across South Africa were able to provide instrumental information and mobilise support for the protests. This thesis focused on exploring the rape culture discourse that emerged from the online debates following the #Endrapeculture protests, as well as the potential of social media as an accessible and affordable pedagogical tool to address rape culture on campus. An exploratory qualitative design was used and this was framed within a postmodern feminist framework. To address the aims of the study two methods of data collection were utilised. All ethics principles were adhered to for both forms of data collection. Firstly, natural observation of comment threads of Facebook relating to the April 2016 #Endrapeculture protests was conducted. A total of 590 comments from 8 Facebook posts were collected and analysed using qualitative content analysis. The findings indicate that rape culture discourses were prominent within these comment threads with Perpetuating Victim-blaming emerging as the most significant theme followed by Rape or Rape Culture, Patriarchy, Race and Culture, Sexualisation and Bodily Autonomy, Trivialising Rape Culture and Role of Universities and Law Enforcement. The second part of the data collection involved conducting online, asynchronous focus groups using the Facebook secret chat group application. Participants for the SFFG were recruited on Facebook through a process of snowball sampling. A total of three SFFG were conducted with 16 participants. Thematic decomposition analysis was used to analyse the data. The findings revealed three main themes namely; Defining Rape Culture, Learning about Rape Culture and The Role of Social Media. Based on the observations from the comment threads and the findings from the SFFGs, it is argued that social media can be used as a pedagogical tool to address rape culture on campus in two ways. Firstly, it is beneficial on a macro level by using social media platforms to provide instrumental information about rape culture. Secondly, it can be utilised on a micro level by using applications like the SFFG to provide a safe space where students can engage in small-scale interactive discussions.

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Brodkin, Kathryn Rhea. "Chondrocyte behavior in monolayer culture : the effects of protein substrates and culture media." Thesis, Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/20216.

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Das, Abhimnanyu S. M. Massachusetts Institute of Technology. "Indian comics as public culture." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/91429.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Comparative Media Studies, 2009.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 83-85).
The Amar Chitra Katha (ACK) series of comic books have, since 1967, dominated the market for domestic comic books in India. In this thesis, I examine how these comics function as public culture, creating a platform around which groups and individuals negotiate and re-negotiate their identities (religious, class, gender, regional, national) through their experience of the mass-media phenomenon of ACK. I also argue that the comics, for the most part, toe a conservative line - drawing heavily from Hindu nationalist schools of thought. In order to demonstrate these arguments, I examine selected groups of ACK titles closely in the first two chapters. I perform a detailed content analysis of these comics, considering the ways in which they draw upon history and primary texts, the artistic and editorial choices as well the implications of these decisions. In the third chapter, I draw a picture of the consumption of these comics, studying the varying interpretations and reactions that fans across generations have had to the works, connecting their conversations to my argument about ACK as public culture. In doing so, I hope to demonstrate the extent of ACK's role in the popular imagination of its large readership as well as the part it plays in the negotiation of their identities as Indians.
by Abhimnanyu Das.
S.M.

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Langlois, Bénédicte. "Culture de l'information, culture de l'image et culture par l'image dans l'enseignement secondaire." Thesis, Montpellier 3, 2019. http://www.theses.fr/2019MON30032.

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L’éducation aux médias et plus particulièrement l’éducation à l’image sont dans les programmes scolaires de l’enseignement secondaire, une éducation à partager, sans programme, sans enseignant référent. L’objectif de ses recherches est de réfléchir à la culture de l'information à construire chez l’élève afin de le rendre plus autonome, de développer son esprit critique pour lui donner la capacité de s’insérer dans une société où l’image est de plus en plus présente. Ce travail propose un parcours d’éducation à l’image photographique au collège et au lycée. Pour ce faire, une étude des textes officiels, des enquêtes auprès des élèves et des enseignants, des entretiens auprès des professionnels ont été menés. Des ressources ont été recensées, des outils pédagogiques créés : une histoire de la photographie, un lexique et un corpus d’images apprenantes conçus L’étude révèle que l’image photographique est un média peu ou pas utilisé par les enseignants pour construire des connaissances, que les élèves ne savent pas décoder ou décrypter une photographie. Une médiation nouvelle a été pensée une médiation documentaire photographique scolaire Une expérimentation a été réalisée en classe de Terminale pour déterminer les compétences nécessaires à développer chez l’élève afin qu’il puisse quitter le système éducatif formé à l’image
Image literacy is part of the school curriculum; it is shared teaching with no official instructions, no referent teacher and no recognition of a specific culture which would allow the pupils to acquire skills and become enlightened actors of our society where image is pervasive. So there is a need to define and build this culture of the image by rooting it in a more recognized information culture attached to a specific school subject and its scientific input. To meet this need, I studied in-depth the official instructions of the Ministry of Education, led surveys and interviews with pupils and teachers to grasp their representations and practices. The concept of image is a broad one, so I focused on the photographic image which is paradoxical: it is extensively used by the pupils in their sociability (eg.selfies) and in the media (magazines, the internet), but at the same time it is very little used by the teachers to build knowledge and even often as mere illustrations in the school books. The consequence is the inability of the pupils to decode and decipher a photograph. At this stage I called upon a third partner - professional photographers. Only they can properly explain the three layers of a photography: its technicity, its aesthetics and its significance. The product of this research work: a collection of existing resources, new pedagogical tools (a lexicon, and a corpus of images chosen for their potential to educate), methods using games, which I was able to experiment with a group of Terminales. The final outcome is a list of the skills to develop throughout the educational path and a whole new triangular mediation: documentary, photographic, and inside the school framework

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Palmer, Daniel Stephen Vaughan. "Participatory media : visual culture in real time /." Connect to thesis, 2004. http://eprints.unimelb.edu.au/archive/00000125.

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Taleb, Hala Abdul Haleem Abu. "Gender, media, culture and the Middle East." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Spring2009/h_abutaleb_042309.pdf.

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Kuritsky, Orit. "Transformational tales : media, makeovers, and material culture." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/46660.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Comparative Media Studies, 2009.
"February 2009."
Includes bibliographical references (p. 94-101).
This thesis probes into current American makeover culture, thorough three detailed case studies that represent an increasing confluence of commerce, entertainment, and, at times, spirituality. Each of the chapters is devoted to a niche media property, or genre, dedicated to the domestic sphere. The first chapter focuses on the genre of home decorating TV shows and practices of their consumption. The second centers on a single television program - TLC's What Not to Wear, and the interpretative activities it provokes among viewers. The third chapter examines the FlyLady - a transmedia property with a strong internet base, described by its founder as a "behavior modification system" that coaches its subscribers in getting their houses in order. This study was driven, among other things, by the following questions: as the 'commodity frontier' gets increasingly intermingled with our daily lives, with the help of increasingly pervasive media, how do certain communities respond, and with what methods of meaning-making? What draws audiences to engage with media properties so intermingled with commerce in the first place? And, what constitutes these properties' entertainment value as well as the other values audiences find in them? The answers vary with each case study, yet, there are many commonalities pertaining to meanings associated with consumer goods in late capitalism. The media properties described here capitalize on the movement of meaning from culture through consumer goods to individuals. At the same time these three chapters exemplify many cases of redirecting, filtering, and damming up the flow of meaning on the part of viewers and subscribers.
by Orit Kuritsky.
S.M.

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Foley, Kimberly Ann. "Perception, aesthetics and culture in new media." Thesis, Massachusetts Institute of Technology, 1988. http://hdl.handle.net/1721.1/73763.

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Bereded-Samuel, Yared. "Ammonia-limited growth of acetobacterium woodii in continuous culture." Thesis, Queen Mary, University of London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294973.

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Tough, Andrew John. "A theoretical model of streptomycete growth in submerged culture." Thesis, University of Aberdeen, 1989. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU553421.

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A theoretical model based on finite elements has been formulated for the growth and development of submerged mycelial pellet populations. Models based on partial differential equations proved to be mathematically unstable. The model is tested experimentally by studying the growth of the filamentous bacterium Streptomyces coelicolor A3(2) in batch and continuous culture. The effects of shear, growth rate and inoculum concentration on population development are considered. All pellets are assumed to grow according to cube root kinetics. Variations in the amount of biomass occurring throughout the width of the peripheral growth zone are accounted for. An inability to accurately simulate variations in pellet strength meant that whilst growth in low shear environments could be simulated well, growth at high shear could only be simulated qualitatively. Biomass yield was directly related to agitation rate, a phenomenon, suggested by the model, to be caused by the influence of agitation on 'pro-pellet' formation. Pellet density was inversely related to pellet radius. Although gross pellet morphology was dependent on medium composition, it was the relative effects of agitation rate and growth rate which determined the form of the pellet size distribution. Antifoam, and pH had no effect on the morphology of pellets grown in shake flasks, though the pellet size distribution was affected. Increasing concentrations of antifoam emulsion above 1&'37 v/v caused a reduction in pellet number, suggesting inhibition of germination, whereas below 1&'37 v/v an increase led to an increase in pellet number suggesting an effect on pro-pellet formation. The effect of increasing pH over a range from 5 to 9 was to reduce pellet number. Modal pellet radius was inversely related to agitation rate and inoculum concentration. Agitation rate influenced the pellet size distribution in two ways (i) by affecting spore agglomeration and hence pro-pellet formation at low shear rates, and (ii) by causing mechanical damage to pellets at high shear rates.

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Salleh, Aishah. "The growth of phytoplankton populations in nature and in culture." Thesis, Royal Holloway, University of London, 1987. http://repository.royalholloway.ac.uk/items/2cb918c8-a73f-4d5d-961c-a0354020224f/1/.

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An investigation over a period of more than two years of phytoplankton, and physical and chemical conditions of the River Thames and the Wraysbury Reservoir in Southern England, has indicated seasonal occurrence of phytoplankton populations with diatoms (chiefly Stephanodiscus ref. hantzschii Grun.) forming a large percentage of the populations. During 1984, 1985 and 1986, phytoplankton populations occurred most abundantly during the spring (mainly diatom populations) and during the summer (diatoms and green algae in the River Thames, and blue-green algae in the Wraysbury Reservoir). Selected algal taxa were isolated and grown in culture in the laboratory and experiments carried out in which culture conditions have been manipulated in various ways. Such experiments included those involving suspected nutrient limiting factors (e.g. phosphate) as well as physical factors especially those of temperature and light. Ecological records and experimental cultures indicate that the occurrence and growth of diatoms is encouraged by the increasing water temperatures and light intensity during the spring, and by higher levels of nutrient concentrations (i.e. nitrate-nitrogen, phosphate-phosphorus and silica) at all times. On the other hand, the growth of green algae (Chlorophyceae) and blue-green algae (Cyanophyceae) was influenced by the maximum water temperatures and light intensity of the summer period and despite lower nutrient concentrations. Thus, differences in physical and nutrient requirements by phytoplankton populations help to explain the presence of diatoms during the spring and green algae (in the River Thames) and blue-green algae (in the Wraysbury Reservoir) during the summer. Simple investigation of the growth responses of the River Thames and Wraysbury Reservoir phytoplankton populations to River Thames and Wraysbury Reservoir water as natural culture media were performed. The results indicated that the River Thames and Wraysbury Reservoir are potentially able to support considerable crops of phytoplankton populations, and that the potential is present throughout the year.

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Artrip, Ryan Edward. "Virulence and Digital Culture." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/80512.

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This dissertation is a theoretical study of the role of virality/virulence as a predominant technological term in the reproduction of social and cultural information in the digital age. I argue that viral media are not new phenomena, only the name is new. Media have always behaved as viruses; it is only when they become hyper-intensified in digital technology that their virulent function surfaces in language and culture. The project examines processes of self-replication and evolution undergone by various new media phenomena as they relate back to the global profusion of social networks, data centers, and cybernetic practices. Drawing from several contributions in media theory, political and social theory, and critical media studies, I argue that digital media have a hyper-intensifying effect on whatever objects, subjects, or realities they mediate or represent; thus networked societies are virulently swarmed by their own signs and images in information. Through an examination of three primary categories of digital proliferation—language, visuality, and sexuality—I situate digital culture in a framework of virulence, arguing that the digital may be best understood as an effect of cultural hyper-saturation and implosion. I argue that virulent media networking processes come to constitute a powerful cybernetic system, which renders the human subject a mere function in its global operations. Lastly, I begin to develop a political critique of cybernetics, claiming that the proliferation of information, digital media, and communicative/representational technologies in the contemporary world emerges through an intensified ideological, economic, social, cultural, and metaphysical framework of productivism. This intensification engenders a system, or series of communicational circuits, whereby all techno-subjective activities are strategically stimulated, networked, recorded, and algorithmically appropriated to strengthen and reproduce 1) a global productivist system of cybernetics; 2) The material and ideological conditions for such a system to exist and thrive; 3) limitless virtual and digital production.
Ph. D.

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Banakis, Renee Michelle. "Media Influence on Perspectives of Deafness as Culture." Miami University Honors Theses / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1114963978.

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Kaskar, Khalied. "Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy." University of the Western Cape, 2021. http://hdl.handle.net/11394/7924.

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Philosophiae Doctor - PhD
The aim of this thesis is first, to evaluate various culture conditions to improve embryo development, and secondly, to analyze spent culture media for any biomarkers that may be predictive of embryo health. Single-step and sequential culture media were compared in both Planer and EmbryoScope™ incubators. Single-step media resulted in better blastocyst development compared to sequential media and the EmbryoScope™ incubation system showed slight improvements in embryo development than the Planer system. The benefits of supplementing the culture medium with either insulin or insulin-like growth factor 1 (IGF-1) or culturing in a 2% O2 environment, using two different strains of mice (hybrid and C57), as well as the suitability of these strains for quality control were compared. In insulin, hybrid embryos were slower to blastulate and had a lower blastocyst rate, whereas C57 embryos were slower to the morula and faster to blastocyst stages, and lower blastocyst rate than the controls. IGF-1 showed no difference in time-lapse morphokinetics (TLM) or blastocyst rates compared to controls in both hybrid and C57 embryos. Under 2% O2, hybrid embryos showed no significant difference in TLM up to the 8-cell stage, but slowed down afterwards, resulting in blastocysts with significantly lower cell counts than the 6% O2 group. The C57 embryos were slower to reach morula and expanded blastocyst, and had lower blastocyst rates in 2%O2 vs 6%O2. The C57 strain had significant slower overall embryo development for all time points than hybrid embryos in insulin, IGF-1 and ultra-low O2, as well as lower blastocyst rates. Measurement of growth differentiation factor 9 (GDF-9) and oxidation-reduction potential (ORP) in spent media as markers for embryo health were evaluated. Day 5 human blastocysts yielded higher pregnancy rates and GDF-9 levels in spent media compared to Day 6 blastocysts, but TLM parameters showed no impact on pregnancy outcome. In Day 6 blastocysts, the non-pregnant group showed significantly faster embryo development compared to the clinically pregnant group up to the 8-cell stage and start of blastulation. GDF-9 did not show any significant differences between non-pregnant and pregnant groups of Day 5 or Day 6 embryo transfers. ORP in spent media from good quality Day 3 embryos that developed into blastocysts were significantly higher than from those that did not, with no difference in control medium ORP. Spent media from arrested embryos showed lower ORP than their corresponding controls. Arrested embryos had slower development at syngamy, morula, blastulation and blastocyst stages. The single step medium in the EmbryoScope™ is the preferred choice for embryo culture. Insulin or IGF-1 media supplementation or 2% O2 culture did not provide any benefit to embryo development. The C57 mouse strain is more sensitive and may be better to detect changes in culture conditions, and therefore better model for quality control assays. GDF-9 values decrease from Day 5 to Day 6 which gives new insight to understanding the role of GDF-9 during embryogenesis. ORP in spent media indicate that embryos that developed into blastocysts did not contribute to ROS, but maintained ORP balance.

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Morris, Pamela Kay Shoemaker Pamela J. "Explicating culture and its influence on magazine advertisements." Related electronic resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2004. http://wwwlib.umi.com/cr/syr/main.

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Tarrant, Patrick Anthony. "Documentary practice in a participatory culture." Queensland University of Technology, 2008. http://eprints.qut.edu.au/26975/.

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Debates concerning the veracity, ethics and politics of the documentary form circle endlessly around the function of those who participate in it, and the meaning attributed to their participation. Great significance is attached to the way that documentary filmmakers do or do not participate in the world they seek to represent, just as great significance is attached to those subjects whose participation extends beyond playing the part of eyewitness or expert, such that they become part of the very filmmaking process itself. This Ph.D. explores the interface between documentary practice and participatory culture by looking at how their practices, discursive fields and histories intersect, but also by looking at how participating in one might mean participating in the other. In short, the research is an examination of participatory culture through the lens of documentary practice and documentary criticism. In the process, however, this examination of participatory culture will in turn shed light on documentary thinking, especially the meaning and function of ‘the participant’ in contemporary documentary practice. A number of ways of conceiving of participation in documentary practice are discussed in this research, but one of the ideas that gives purpose to that investigation is the notion that the participant in contemporary documentary practice is someone who belongs to a participatory culture in particular. Not only does this mean that those subjects who play a part in a documentary are already informed by their engagement with a range of everyday media practices before the documentary apparatus arrives, the audience for such films are similarly informed and engaged. This audience have their own expectations about how they should be addressed by media producers in general, a fact that feeds back into their expectations about participatory approaches to documentary practice too. It is the ambition of this research to get closer to understanding the relationship between participants in the audience, in documentary and ancillary media texts, as well as behind the camera, and to think about how these relationships constitute a context for the production and reception of documentary films, but also how this context might provide a model for thinking about participatory culture itself. One way that documentary practice and participatory culture converge in this research is in the kind of participatory documentary that I call the ‘Camera Movie’, a narrow mode of documentary filmmaking that appeals directly to contemporary audiences’ desires for innovation and participation, something that is achieved in this case by giving documentary subjects control of the camera. If there is a certain inevitability about this research having to contend with the notion of the ‘participatory documentary’, the ‘participatory camera’ also emerges strongly in this context, especially as a conduit between producer and consumer. Making up the creative component of this research are two documentaries about the reality television event Band In A Bubble, and participatory media practices more broadly. The single-screen film, Hubbub , gives form to the collective intelligence and polyphonous voice of contemporary audiences who must be addressed and solicited in increasingly innovative ways. One More Like That is a split-screen, DVD-Video with alternate audio channels selected by a user who thereby chooses who listens and who speaks in the ongoing conversation between media producers and media consumers. It should be clear from the description above that my own practice does not extend to highly interactive, multi-authored or web-enabled practices, nor the distributed practices one might associate with social media and online collaboration. Mine is fundamentally a single authored, documentary video practice that seeks to analyse and represent participatory culture on screen, and for this reason the Ph.D. refrains from a sustained discussion of the kinds of collaborative practices listed above. This is not to say that such practices don’t also represent an important intersection of documentary practice and participatory culture, they simply represent a different point of intersection. Being practice-led, this research takes its procedural cues from the nature of the practice itself, and sketches parameters that are most enabling of the idea that the practice sets the terms of its own investigation.

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Okarski, Kevin M. "Novel Point-of-care disposable device and cell culture bioprocessing technique." Thesis, The University of Arizona, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10134160.

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This dissertation is composed of two projects dedicated to the development of techniques and technologies for improving the quality of life for patients in both clinical and resource-limited settings.

The purpose of the first project was to design a rapid diagnostic device to screen whole blood samples for the presence of infectious agents. Point-of-care (PoC) technologies are becoming increasingly important for the detection of infectious agents in resource-limited settings (RSLs) where state-of-the-art blood screening practices are not feasible for implementation. For this project, a rapid diagnostic device was developed to directly detect pathogen content within freshly drawn whole blood samples using a ligand-binding assay format. The assay is completely self-contained within a hermetically sealed device to minimize operational complexity and ensure operator safety. The diagnostic device is capable of processing complex sample matrices by selectively capturing, concentrating, and labeling infectious agents upon functionalized surfaces. Following sample processing, the assay is optically interrogated with a fluorescence-based reader to provide rapid feedback regarding sample purity. Designs of the rapid diagnostic platform evolved over several prototype generations corresponding to project milestones emphasizing ergonomic performance, military specification testing for environmental resilience, and manufacture to yield production-grade devices for future diagnostic performance data collection.

The goal of the regenerative therapy-based portion of this research was to develop a novel technique for the selective enrichment of cells demonstrating enhanced regenerative capacity in tissue-extracted cell samples. Adherent cell cultures of stromal vascular fractions (SVFs) extracted from adipose tissues were exposed to nutrient deficient conditions – eliciting a bimodal cellular response between two dissimilar cell culture subpopulations. The regenerative capacity of these two distinct subpopulations was evaluated by assessing their characteristic morphology, metabolic activity, and ability to undergo multilineage differentiation. The SVF subpopulation which demonstrated sensitivity to the nutrient deficient conditions expressed typical morphological expression of adherent cell cultures, elevated metabolic activity, and the ability to differentiate along adipogenic, chondrogenic, and osteogenic lineages. The SVF subpopulation which demonstrated resistance to the nutrient deficient conditions, however, expressed atypical morphologies, impaired metabolic activity, and did not survive culture with differentiation growth media. Based on the data, the ‘treatment-sensitive’ SVF subpopulation demonstrated a greater regenerative capacity than the ‘treatment-resistant’ subpopulation. Furthermore, the treatment-resistant subpopulation of the SVF may be representative of the damaged, senescent, and otherwise less-functional cells that comprise a significant portion of tissue-extracted cell samples and pose a significant risk to therapeutic efficacy and reproducibility. Ultimately, this expedient and inexpensive bioprocessing technique may serve to improve cell-based regenerative therapies by eliminating undesirable cells from culture.

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Carver, Sarah Marie. "Characterization of a Thermophilic, Cellulolytic Microbial Culture." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299687326.

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Piccinotti, Silvia. "Rabies Glycoprotein-Mediated Uptake Into Epithelial Cells and Compartmentalized Primary Neuronal Culture." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:23845493.

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Rabies virus (RABV) subverts host neuronal circuitry to gain access to the brain where it causes generally incurable, lethal encephalitis. The single glycoprotein (G) dictates two defining steps for infection and neuroinvasion: receptor-mediated endocytosis and transport of virus. We generate two recombinant VSV (rVSV) clones that genetically incorporate G (rVSV RABV G) from the fixed RABV strains, SAD B19 and CVS, to study internalization into epithelial cells and compartmentalized primary cultures of peripheral neurons. Through the use of chemical inhibitors and markers for specific endocytic routes, we demonstrate that the predominant RABV entry route in both epithelial and neuronal cells is dynamin- and clathrin- dependent. Viral endocytosis is mediated by actin-dependent, partially coated clathrin pits as evidenced by live high resolution confocal microscopy of envelopment in epithelial cells and transmission electron micrographs in neuronal and non-neuronal cells. Thus, we corroborate the hypothesis that particle size is the sole viral determinant of actin-dependence of coated pits. Through a combination of high resolution microscopy and infectivity-based approaches, we link molecular mechanisms of viral uptake at the single particle level to productive infection. Targeted pharmacological disruption of endosomal acidification at the neurites or cell bodies of peripheral neurons demonstrates that fusion and viral genome release at the cell body, the site of replication, is a prerequisite for infection. This work extends the current understanding of RABV entry by providing a detailed characterization of endocytosis from the plasma membrane to the site of fusion and correlating it with establishment of infection into neuronal populations relevant for pathogenesis in vivo.
Biology, Molecular and Cellular

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Dinnen, Zara. "Mixed media : representing the digital in contemporary American culture." Thesis, Birkbeck (University of London), 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.590625.

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As we continue to move through a moment of pervasive digital culture, and increasingly ubiquitous digital technology, new aesthetic paradigms emerge across the arts. This thesis explores those paradigms, representations of the digital, as they appear in contemporary American culture. Rather than reflecting on this concern through the parameters of digital texts, this thesis will develop an expanded idea of digital culture, one that includes print and analogue works that reflexively engage with the digital. The digital environments we negotiate today are culturally rooted in the US. Despite its history, the presence of digital culture as a formative aspect of contemporary American literature and art has been little explored from within the discipline of American studies. This thesis will argue that more sustained critical attention is needed to consider how the digital emerges as a subject of American culture since 2000. Beginning with a study of the status of the book in the digital age, this thesis contends with its subject through examinations of remixing in literature, and of the representation of code in visual culture, before moving on to consider themes of location and identity in networked environments. It will provide close readings of a range of texts: books by the publishers Mcsweeney's; literary works by Mark Amerika, Jennifer Egan, Robert Fitterman, Jonathan Lethem, Richard Powers, and Gary Shteyngart; the films The Social Network and Catfish; and artworks by Cory Arcangel, Eva and Franco Mattes, and Takeshi Murata. This thesis will argue that the digital is a key subject for American culture of the last fifteen years. It will consider the digital as materially, and formatively, embedded in culture. To address the complexity of this approach, this thesis will study contemporary American literature and art through the lens of digital theory.

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Rutherford, Marc A. "Mass media framing of hip-hop artists and culture." Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=1974.

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Stern, Savannah. "Suicide and Suicide Prevention in Media and Mass Culture." Scholarship @ Claremont, 2019. https://scholarship.claremont.edu/cmc_theses/2030.

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With suicide on the rise nationwide, it is important now more than ever to prioritize suicide as a public health issue. This means raising awareness and conducting research aimed at developing new suicide prevention tools and strategies, as well as reevaluating and challenging already existent ones. Media messaging can be a great suicide prevention tool. Suicide depictions and reporting in different forms of media—including newspapers, online publications, film, television, and more—have the power to influence behavior. When reporting in a safe and appropriate manner, the media can influence behavior in a positive way and encourage help-seeking. However, reports that sensationalize and glamorize suicide have the potential to spark suicide contagion. Thus, when reporting on suicide it is crucial to be aware of best practices and recommendations developed by experts. In recent years, media campaigns aimed at suicide prevention have gained traction. While there has been some evidence suggesting the success of such campaigns, more research is needed in this area. Further research is also needed to assess the effects of fictional depictions of suicide in film and television.

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Alfaro, Alfonzo Antonio Alejandro. "Metabolomics study of human embryonic stem cell culture media." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/28850/.

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Self-renewal and pluripotency, the hallmarks of human embryonic stem cells (hESC), confer these cells with the capacity to expand indefinitely while maintaining the ability to differentiate into any cell type of the human body; thus, making hESC a valuable source of functional differentiated cells suitable for applications in regenerative medicine, drug discovery, biotechnology, biopharmaceuticals and developmental biology. However, the large-scale production of clinical-grade hESC, required for such applications, has been hampered by the current culture conditions in which hESC still depend on the use of mouse embryonic fibroblast-conditioned medium (MEF-CM) for their efficient growth. Therefore, investigation of the factors provided by MEFs is of the utmost importance to discover which components of MEF-CM allow the long-term expansion of undifferentiated hESC. While considerable progress has been made on the identification of the protein components of MEF-CM, very little is known about the small molecules (metabolites) secreted by MEFs. In this context, an untargeted metabolomics method was developed for the investigation of potential bioactive metabolites present in MEF-CM implicated in the proliferation and/or maintenance of pluripotency of hESC in vitro. A metabolomics method was applied and successfully identified a number of metabolites which were later confirmed in their identities with the use of authentic standards, to be further investigated for their effect on hESC culture. Interestingly, the addition of PGE2, 6-keto-PGF1α, 9, 12, 13-TriHOME, 7-Ketocholesterol and stearidonic acid (the metabolites found in MEF-CM) to the unconditioned medium (UM), a medium incapable of the maintenance of hESC, showed a delay in apoptosis when compared to the negative control UM; thus, suggesting that these metabolites could help with the proliferation of hESC. Increasing evidence that hESC secrete factors into their microenvironment that can also help them to proliferate or to maintain an undifferentiated state prompted the application of the same metabolomics method to the analysis of hESC spent culture media. The results identified lysophospholipids (LPLs) as potential molecules mediating some biological activities; however, the precise role of these LPLs still remains to be determined. Overall, the results of this thesis are expected to impact and add knowledge to the field of stem cell biology providing useful information for the creation and development of more efficient and defined culture conditions for the propagation of hESC with the appropriate quality to realise their widespread application in clinic and other research areas.

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Omar, Hadeer. "Egyptianization: Culture hacking as a method." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4114.

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In a broad sense, cultures undergo a metamorphoses due to the external influences and systems impacting the evolution of internal identities. Conversely, Individuals within cultures react to those external influences and systems.The act of hacking a culture is an opportunity to challenge an existing or imported system in order to bring about change and improvement. An aspect of culture hacking is to create messages of satire or irony in order to criticize, or completely reject established systems within cultures. Post Arab spring, Egyptians practiced culture hacking by applying their cultural tools to external systems and influences, producing a process of ‘Egyptianization’. This investigation examines the MFA program’s culture at Virginia Commonwealth University in Qatar as a case study, and adapts those Egyptianized tools to hack the culture. The program has its own values, rituals, traditions, imported systems, influences and dominant symbols. The aim of this thesis is to generate customized hacking methodologies that identify cracks within this culture and develop an innovative framework to critically analyze them through visual representation.

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Nordström, Niklas. "Organizational culture in Slack : The relationship between organizational culture and digital collaboration tools." Thesis, Karlstads universitet, Avdelningen för medie- och kommunikationsvetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-72399.

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The purpose of this study was toexplore organizational culture in a digital context, so that a greater understanding of the relations between the two subjects could be developed. The interest for the subject came from reflections and observations obtained during a prior internship at a small organization using the digital collaboration platform Slack in their daily work. To answer the purpose of the study, one main research question; ‘What is the relationship between organizational culture and a digital communication platform as Slack?’ and two sub questions; ‘How is Slack used to solve problems with internal integration? and ‘How is Slack used to solve problems with external adaption?’ was formulated. The two sub questions came from an operationalization of Schein’s (2010, p.18) well used definition of organizational culture. By using the qualitative method netnography to study the behaviors and interactions of the members of a small organization through participating observations, in combination with the field notes and observations from the prior internship, the research questions were successfully answered. The results showed that Slack was used as a tool to maintain structure and order during problems with external adaption in form of a re-organization creating an unsecure time-period. Decrement in activity also showed that the usage of Slack was limited in time and easily could be exchanged, but that appreciated cultural rites and behaviors created from using it could live on outside of Slack. Results also showed that Slack successfully functioned as a tool to solve problems with internal integration. By allowing new members to efficiently come in contact with both the formal and informal cultural elements, the very essence of culture as in underlying assumptions was quickly taught. The efficiency of using Slack for internal integration was also shown to rely on a new possible problem in form of a disintegration between the private and work. The answer to the main research question was that Slack is an artifact, inhabiting other artifact, living in symbiosis with the organization. Even though Slack could help an organization to cope with problems of external adaption and internal integration, Slack on its own did not serve as a one single place for understanding and becoming a part of an organization and its culture, as the organization and culture still will live and develop outside of the digital medium.
Syftet med den här studien var att utforska organisationskultur i en digital miljö, så att en ökad förståelse för de två ämnena kunde utvecklas. Intresset för ämnet kom från observationer och reflektioner införskaffade under en tidigare praktik på en mindre organisation som använde den digitala plattformen Slack i sitt dagliga arbete. För att besvara syftet med studien formulerades en huvudsaklig forskningsfråga; ’Vad är relationen mellan organisationskultur och en digital plattform som Slack?’, och två sekundära frågor; ’Hur används Slack som lösning för problem med intern integration?’, och ’Hur används Slack för att lösa problem med extern anpassning?’. De sekundära frågorna kom från en operationalisering av Scheins (2010, p.18) väl använda definition av organisationskultur. Den kvalitativa metoden netnografi användes för att studera beteende och interaktioner mellan medlemmarna i en mindre organisation. Genom deltagande observationer i kombination med fältanteckningar och observationer från den tidigare praktiken kunde forskningsfrågorna framgångsrikt besvaras. Resultatet visade att Slack användes som ett verktyg för att behålla struktur och ordning under problem med extern anpassning, uppkomna till följd av en omorganisering av företaget. En minskning av aktiviteten i Slack visade att själva användandet av Slack är kopplat till en viss tidsperiod och enkelt kan bytas ut vid förändrat behov, men också att uppskattade beteenden och riter skapade genom användandet av Slack kan leva vidare utanför mediet. Resultatet visade också att Slack framgångsrikt fungerade som ett verktyg för att lösa problem med intern integration. Genom att låta nya medlemmar effektivt komma i kontakt med både formella och informella kulturella element kunde själva essensen av kultur, underliggande förgivettaganden, snabbt läras ut. Effektiviteten av att använda Slack för intern integrering visades också föra med sig ett eget potentiellt problem, en upplösning av gränsen mellan privat och arbete. Svaret på den huvudsakliga forskningsfrågan var att Slack är en artefakt, innehållandes andra artefakter, som lever i symbios med organisationen. Även om Slack kan hjälpa en organisation att hantera problem med extern anpassning och intern integrering, fungerar Slack inte som en ensam källa för att förstå och bli en del av en organisation och dess kultur, eftersom organisationen och dess kultur alltid kommer att leva vidare och utvecklas utanför det digitala mediet.

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Arvidsson, Amanda. "Cancel culture - amatörmässig rättvisa : En kvalitativ intervjustudie om unga vuxnas deltagande i internetfenomenet cancel culture på sociala medier." Thesis, Karlstads universitet, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-84546.

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One measure that groups of people have always taken is to ostracize the individuals who violate social norms. This is still going on today, even in digital form. Cancel culture is a prevailing internet phenomenon on social media where public figures are banned from influence after saying or performing a, according to the public, wrongful act. The phenomenon is, to say the least, debated and controversial but nevertheless lacks detailed scientific work. The purpose of this study is to investigate young adults' participation in cancel culture and to shed light on possible reasons why they maintain and conduct cancel culture on social media, which was carried out with the help of the main question: - How do young adults in Sweden reason about their participation in cancel culture on social media and why do they choose to participate? Through a qualitative interview survey, where eight respondents shared their experiences and opinions on their participation, conclusions could be drawn. The results showed that the interviewees' reasoning was positive in the sense that their participation had positive consequences for themselves and that they participate partly to show their position and thus be part of the group, and partly to command the justice that the judiciary has failed to do.
En åtgärd som grupper av människor alltid har vidtagit är att frysa ut de individer som bryter mot de sociala normerna. Detta pågår än idag, även i digital form. Cancel culture är ett rådande internetfenomen på sociala medier där offentliga personer blir bannlysta från inflytande efter att ha sagt eller utfört en, enligt allmänheten, felaktig handling. Fenomenet är minst sagt omdebatterat och kontroversiellt men saknar trots detta utförligt vetenskapligt arbete. Den här studien har i syfte att undersöka unga vuxnas medverkan i cancel culture samt belysa möjliga orsaker till varför de upprätthåller och bedriver cancel culture på sociala medier, vilket genomfördes med hjälp av huvudfrågeställningen: - Hur resonerar unga vuxna i Sverige kring sin medverkan i cancel culture på sociala medier och varför väljer de att delta? Genom en kvalitativ intervjuundersökning, där åtta respondenter berättade om sina upplevelser och åsikter kring sin medverkan, kunde slutsatser dras. Resultatet visade på att intervjupersonernas resonemang var positiva i den bemärkelsen att deras deltagande fick positiva konsekvenser för dem själva och att de medverkar dels för att visa sin ståndpunkt och därmed vara en del av gruppen, och dels för att själva skipa den rättvisa som rättsväsendet misslyckats med.

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35

Nelson, Wade Gordon James. "Reading cycles : the culture of BMX freestyle." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=102820.

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This dissertation draws from and contributes to many traditions within the (interdisciplinary) discipline of communication studies. Serving the two primary objectives of the examination of the figure of the BMX freestyle cycling Pro and the analysis of the role of the magazines within this particular culture or field in the construction and maintenance of this figure, this project brings together studies of cultural intermediaries, magazine history, advertising history and theory, subcultures, audiences, commodification, cultural industries, celebrity, stars and professional athletes. The culture of BMX freestyle cycling is an interesting and heretofore unexamined phenomenon, and a focused examination allows the exploration and investigation of larger questions within the discipline. As such, this dissertation provides an informed interpretation of the culture of BMX freestyle, allows the examination of a number of other issues concerning the mediation of cultural practices, and suggests a theory of the special-interest magazine, thus contributing substantively to various literatures.
Special-interest magazines are a part of a larger system and industries within which the ultimate goal is the sale of commodities. At the same time, they function as a site of credibility within a larger field, both conferring star status on particular individuals and approving particular commodities that are being offered to the readers. Special-interest magazines construct and sell audiences to advertisers, create star systems, propose candidates for stardom, help build image careers, contribute substantially to a "star currency" within the particular field, negotiate (i.e.; mediate) tensions between the advertisers, the stars, and the readers, help organize the time of a culture and work to infuse it with a sense of vitality through the punctual and ritualistic appearance of novel content, assist the consumer with their desires for commodities and stars by standing as catalogues of commodities (serving to educate newcomers in the protocol of the culture), provide new financial opportunities (such as the commodity form of the photo contingency), and in their complicity with the needs of those that provide their primary source of revenue, give more value to the advertising dollar in the construction of editorial content that could be seen as advertising.

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Kim, Sae-Eun. "Communication, culture and the Korean public sphere." Thesis, University of Sussex, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324185.

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The aim of this thesis is to analyse the public communication activities of Korean people from the Chason dynasty to the present day using the conceptual category of the public sphere theorised by Jurgen Habermas. It is mainly concerned with two fundamental issues: the issue of 'communication and democracy,' and that of 'communication and culture.' Emphasising tradition and culture as among the most significant elements in the consideration of communicative action and the public sphere in the Korean context, the thesis takes issue with the claims to universality in Habermas's theory. My argument is that Habermas's theory cannot easily be applied to non-Western societies unless there is sufficient consideration of their idiosyncratic traditions and cultures. To develop this argument, the thesis addresses the impact of Confucianism on speech acts in Korea and the extent of their difference from those in a Western context. In identifying 'silence' as a key term, the situation of women in Korean cultures is particularly pertinent. The second consideration is the question of political authoritarianism which is responsible for the repression of free expression of opinion in collusion with Confucianism. I have discovered that several kinds of public domains of communication have developed through Korean history, despite those two repressing mechanisms, Confucianism and political authoritarianism, public domains which I suggest are more appropriately called 'the public sphere' according to Habermas's terminology. It is meaningful to filter and interpret various communication activities across historical periods from within the analytic framework of the public sphere. In relation to modem Korea, the thesis focuses on the media-saturated public sphere and the current civil movements to demonstrate the dynamics between power and money and their impact on the democratisation process

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Lynch, Hilary. "A study of cyclic fed batch culture for the production of secondary metabolites." Thesis, University of Surrey, 1995. http://epubs.surrey.ac.uk/844465/.

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The broad aim of this research was to evaluate a cyclic fed batch culture system for the production of secondary metabolites and to identify a physiological parameter that correlated with product induction for optimisation of the process. A cyclic fed batch culture (CFBC) system was successfully established using a constant flow of glucose limited medium into the bioreactor, and culture stability was found to be dependent on time of flow initiation. A 40% increase in product concentration was obtained using CFBC when compared to batch culture with the organism Saccharopolyspora erythraea. CFBC induced secondary metabolite production with the organism Amycolatopsis orientalis, despite the lack of production in batch culture. Product concentration was dependant on the growth rate range used in CFBC for both organisms, although the trends of productivity differed for the two. Product stability at quasi steady-state during CFBC depended on the variation in biomass concentration obtained. A number of physiological parameters were measured in both batch culture and CFBC for correlation with product induction. A decrease in protein synthesis rate was observed to precede the induction of antibiotic in CFBC with both organisms, however, this trend was not observed in batch culture with Amycolatopsis orientalis. Although complex trends were observed for levels of RNA, total protein and the ratio of the two, no correlation with induction was observed. Profiled feeding regimes were employed for the manipulation of protein synthesis rates to optimise conditions for production. Increased productivity was not obtained, although protein synthesis rate was manipulated to reduce the timing of product induction at the start of the cycle. Morphological examination of the filamentous organism Saccharopolyspora erythraea, grown in either CFBC, chemostat culture or variable volume-chemostat culture, was found to indicate the organisms' physiological state. Productivity was highest in CFBC, coinciding with a transition from exponential to linear morphological growth and decreasing growth rate through the progress of one cycle. Where the morphological growth rate remained constant, that is in variable volume chemostat, productivity was lowest. In chemostat culture a morphological distribution was observed with both exponential and linear growth resulting in a product concentration higher than found in variable volume chemostat but less that in CFBC. Future work should include molecular studies to determine events leading to secondary metabolite induction. In conjunction with these studies development and unstructured model of the physiology is required. Clarification of the role of protein synthesis rates and particular components of the mechanism are required before this parameter can be discarded.

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Ingvoldstad, Bjorn Paul. "Post-socialism, globalization, and popular culture 21st century Lithuanian media and media audiences /." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3219906.

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Thesis (Ph.D.)--Indiana University, Dept. of Communication and Culture, 2006.
Source: Dissertation Abstracts International, Volume: 67-06, Section: A, page: 1962. Adviser: Barbara Klinger. "Title from dissertation home page (viewed June 21, 2007)."

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39

Watkins, Sean Edward. "Media Literacy and the Digital Age." Bowling Green State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1242223666.

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40

Iyimoga, Christopher Okuba. "Broadcasting and the traditional media in Nigeria." Thesis, University of Leicester, 1986. http://hdl.handle.net/2381/34592.

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41

Tacchi, Jo Ann. "Radio sound as material culture in the home." Thesis, University College London (University of London), 1997. http://discovery.ucl.ac.uk/1317663/.

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This thesis is an anthropological exploration of the contemporary role and use of radio sound in the home in Bristol, a city in the south west of England. Based on qualitative research, and taking an ethnographic approach, this study contributes to a growing field within social anthropology: the study of mass media. After establishing the ways in which the radio industry in the UK researches and constructs radio audiences, this thesis examines how academic research on audiences has operated in Britain. It is demonstrated how this thesis relates to, and is different from both of these perspectives. Radio sound is approached as a part of the material culture of the home. It is seen to contribute to domestic soundscapes. The medium of sound is investigated, and it is shown that radio sound has particular qualities that make it well suited to domestic, everyday life. It is revealed as aiding in the creative constitution of affective dimensions of the self in society. Domestic relationships, and the role of radio sound and affect are explored. Notions of intimacy and the role of fantasy in domestic relationships are investigated. Radio sound's role in mood creation for individuals in the home is then examined, and the notion of affective rhythms established. Radio sound's connecting powers are then given some attention; how radio sound helps to make links across time and space. Memories and nostalgia are shown to operate in creative and integrated ways in domestic contexts through the medium of sound. Finally, it is concluded that cultural knowledge and experience take place in large part in the sensory and affective dimensions of everyday life.

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42

Zafer, Ahmed Abu. "Mycobactericidal testing of chemical germicides, comparison of conventional culture with a reporter gene assay." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0019/MQ48191.pdf.

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43

Marsh, Rachel Angela. "Genotypic and phenotypic changes in human respiratory syncytial virus on passage in cell culture." Thesis, University of Newcastle Upon Tyne, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275527.

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44

Stone, David Michael. "Strain dependent variation in the replication of Theilers Murine encephalomyelitis viruses in cell culture." Thesis, University of York, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328462.

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45

Kisil, Gerry. "Technologies of abundance, consumer culture, government and the media arts." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape15/PQDD_0004/MQ39936.pdf.

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46

Wittenbach, Amy. "Online sports culture finding the sacred in new media environments /." CONNECT TO ELECTRONIC THESIS, 2007. http://dspace.wrlc.org/handle/1961/4278.

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47

Fahmy, Ziad Adel. "Popularizing Egyptian Nationalism: Colloquial Culture and Media Capitalism, 1870-1919." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/195746.

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In Egypt, during the late nineteenth-early twentieth century, older, fragmented, and more localized forms of identity were rapidly replaced with new alternative concepts of community, which for the first time, had the capacity to collectively encompass the majority of Egyptians. The existing historiography however, places Egyptian nationalism exclusively within the realm of elite politics. Thus, this dissertation seeks to investigate the agency of ordinary Egyptians in constructing and negotiating national identity. The principal reason why the Egyptian urban masses are not well represented in the literature is the almost complete neglect of colloquial Egyptian sources. Indeed, I would contend that writing a history of modern Egypt without taking into account colloquial Egyptian sources is, by default, a top-down history and will at best provide only a partial understanding of Egyptian society.This study has several simultaneous objectives. The first is to highlight and feature the role and importance of previously neglected colloquial Egyptian sources--be they oral or textual--in examining modern Egyptian history. This, I argue, is crucial to any attempt at capturing the voice of "ordinary" Egyptians. The second objective is to document the influence of a developing colloquial Egyptian mass culture as a vehicle and forum through which, among other things, "hidden transcripts" of resistance and critiques of colonial and elite authority took place. And lastly, through the lens of colloquial mass culture, this study traces the development of collective Egyptian identity, and the strengthening of Egyptian national communality from the 1870s to the 1919 Revolution.

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Jansson, André. "Image culture : media, consumption and everyday life in reflexiv modernity /." Göteborg : JMG, 2003. http://catalogue.bnf.fr/ark:/12148/cb391513644.

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49

Andersson, Victoria, and Louise Jandér. "Social Media, Insta-Culture and The Reinvention of Fashion Week." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-10147.

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Background: It is clear that the fashion industry is experiencing a change as a result of the explosion of social media. Today the four key social media platforms for fashion houses are Instagram, Facebook, Twitter and Snapchat. The society has created a culture around these media platforms, which is referred to as insta-culture. Why read Vogue magazine to find out the latest trends, when social media is covering the runways in real time? A debate about what direction the fashion industry is heading towards is a hot topic of discussion. The fashion industry has gone through changes before but now consumers have become more demanding regarding quick accessibility of fashion. Consumer behavior and the way we consume fashion has changed. An obvious sign of the change within the industry is how fashion weeks around the world have become the most coveted”it” event for celebrities and fashionistas worldwide. Fashion week is a fashion industry event that lasts around one week. The goal is for the fashion industry to network and for fashion houses to share their next season collections. Fashion weeks have traditionally been a closed, trade only-event which highlights promotional and glamorous entertainment events. However, now it is the most important shows that everyone wants to be seen at. What will become of fashion weeks is truly unknown. One thing that is for sure is that the development of social media definitely plays a vital role in the change of the industry. Aims of the research: The broader aim of this research is to analyze through the lens of fashion week, what is happening in fashion week and the driver of change within the fashion industry, social media, and to unravel why we see a change in fashion week now. The second aim is also to investigate in what way the change is affecting the way fashion is displayed at fashion weeks. Methods: This thesis was completed by gathering observations and analyzing interviews, blogs, press, journal articles, social media and observations that focused on the phenomenon of fashion week especially in the New York and Stockholm context. This qualitative method is referring to as Netnography using primarily data gathering from the internet and data from the existing interviews with people from the industry. Results: Social media has affected the fashion weeks in many ways. Today fashion brands have to include social media in their marketing strategy in order to survive in the insta-culture that reflects on the society. The insta-culture has as well resulted in that fashion week has been reinvented to an entertainment event open for everyone. The democratization of the fashion week has in turn resulted in that designers have to change how and when they deliver fashion. Instead of waiting six months for the collections to hit the stores, designers now have adopted a see now buy now model. Contributions: The result of this thesis brings awareness to people within the fashion industry and fashion theories when it comes to what is happening to the industry and how to better support fashion management strategies. Social media has created an insta-culture in the society that also affects the fashion industry. This thesis also highlights the great impact that the society has on fashion even today. This study therefore further develops the sociological theory when it comes to the impact from the society and how it affects fashion.

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Papadaki, Eirini. "The mediation of art through the mass media." Thesis, University of Kent, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.246640.

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Dissertations / Theses on the topic 'Microbiology - Culture media and culture'

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