Relevant bibliographies by topics / Microbiology – Cultures and culture media

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Academic literature on the topic 'Microbiology – Cultures and culture media'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 January 2023

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Journal articles on the topic "Microbiology – Cultures and culture media"

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Kelly, Devin, Julie Rizzo, Heather Yun, and Dana Blyth. "Microbiology and Clinical Characteristics of Industrial Oil Burns." Open Forum Infectious Diseases 4, suppl_1 (2017): S109. http://dx.doi.org/10.1093/ofid/ofx163.111.

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Abstract Background Injured oil workers are exposed to a broad microbiome in hydraulic fracturing fluids (HFF) and oil wells at the time of injury. This includes Pseudomonas, Stenotrophomonas, Acinetobacter, and rare human pathogens which may be harder to culture. This study evaluates oil-related burn (ORB) microbiology. Methods Patients admitted to the USAISR burn center enrolled in the Epidemiology of Workplace Burns and Injuries in Texas registry from April 2011 to November 2016 were included as cases and controls. Patients hospitalized ≤2 days were excluded. ORB was defined as exposure to HFF (FORB), or non-HFF (NFORB). Controls were patients admitted with industrial burns (non-ORB). Patient demographics and clinical cultures (days 1–15) were obtained through the registry and electronic medical record. Results 149 industrial burns were included, of which 35 (23%) were ORB and 114 (77%) were non-ORB. Of the ORB, 11 (31%) were FORB and 24 (69%) were NFORB. ORB had a median age, TBSA, and Baux score of 31, 25, and 58 compared with non-ORB with 36, 4, and 44, respectively (P < 0.01). Twenty-five patients had positive cultures: 12 (48%) non-ORB and 13 (52%) ORB. Sixty Isolates identified from the ORB population included Flavobacterium, Pseudomonas, and Serratia. FORB accounted for three (25%) of the culture positive ORB. S. marcescens was isolated in 1 FORB (33%) compared with 0 NFORB and non-ORB (P < 0.05). Otherwise, there was no statistical difference in isolates. Median time to first positive culture differed among non-ORB (4 days), FORB (13 days), and NFORB (3.5 days, P = 0.03). Forty-six (31%) patients had cultures obtained during admission: three (7%) FORB, 12 (26%) NFORB, and 31 (67%) non-ORB. Of cultured patients, ORB had a median TBSA and Baux score of 44 and 90 compared with non-ORB with 11 and 47, respectively (P < 0.01). Comparing all cultured patients, ORB had more positive, negative, and total cultures compared with non-ORB with 2 vs. 0, 7 vs. 3, and 10 vs. 3, respectively (P < 0.01). Conclusion Within this cohort, ORB was associated with more severe injuries compared with non-ORB. They had more positive, negative, and total cultures, and recovery of S. marcescens was associated with FORB. Larger studies with non-culture based technology could help further define the microbiology of this uniquely exposed population. Disclosures All authors: No reported disclosures.
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Merlino, John. "Applications and integration of chromogenic culture media in clinical microbiology." Microbiology Australia 31, no. 3 (2010): 127. http://dx.doi.org/10.1071/ma10127.

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The ability to rapidly detect and identify micro-organisms is of paramount importance for many microbiology laboratories. The application of new classes of enzymatic-based chromogenic compounds has revolutionised traditional culture media, leading to the developments of a new generation of chromogenic media. Many studies have shown that the chromogenic agar has become more than an isolation media when compared to conventional culture media. A newer, faster, more cost-effective means in the presumptive identification or screening of microorganisms which are either genus, or in some cases species-specific, and allows superior differentiation of mixed micro-organisms in cultures. When supplemented with antibiotics or other agents, chromogenic media allows the screening for multidrug resistance and virulence in many micro-organisms and can be easily integrated with other automated and/or molecular-based methods.
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Connon, Stephanie A., and Stephen J. Giovannoni. "High-Throughput Methods for Culturing Microorganisms in Very-Low-Nutrient Media Yield Diverse New Marine Isolates." Applied and Environmental Microbiology 68, no. 8 (August 2002): 3878–85. http://dx.doi.org/10.1128/aem.68.8.3878-3885.2002.

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ABSTRACT Microbial diversity studies based on the cloning and sequencing of DNA from nature support the conclusion that only a fraction of the microbial diversity is currently represented in culture collections. Out of over 40 known prokaryotic phyla, only half have cultured representatives. In an effort to culture the uncultured phylotypes from oligotrophic marine ecosystems, we developed high-throughput culturing procedures that utilize the concept of extinction culturing to isolate cultures in small volumes of low-nutrient media. In these experiments, marine bacteria were isolated and cultivated at in situ substrate concentrations—typically 3 orders of magnitude less than common laboratory media. Microtiter plates and a newly developed procedure for making cell arrays were employed to raise the throughput rate and lower detection sensitivity, permitting cell enumeration from 200-μl aliquots of cultures with densities as low as 103 cells/ml. Approximately 2,500 extinction cultures from 11 separate samplings of marine bacterioplankton were screened over the course of 3 years. Up to 14% of the cells collected from coastal seawater were cultured by this method, which was 14- to 1,400-fold higher than the numbers obtained by traditional microbiological culturing techniques. Among the microorganisms cultured were four unique cell lineages that belong to previously uncultured or undescribed marine Proteobacteria clades known from environmental gene cloning studies. These cultures are related to the clades SAR11 (α subclass), OM43 (β subclass), SAR92 (γ subclass), and OM60/OM241 (γ subclass). This method proved successful for the cultivation of previously uncultured marine bacterioplankton that have consistently been found in marine clone libraries.
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Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat, and Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures." Biology 10, no. 7 (June 22, 2021): 569. http://dx.doi.org/10.3390/biology10070569.

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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.
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Libertin, Claudia R., Keith A. Sacco, and Joy H. Peterson. "Education and coaching to optimise blood culture volumes: continuous quality improvement in microbiology." BMJ Open Quality 7, no. 3 (July 2018): e000228. http://dx.doi.org/10.1136/bmjoq-2017-000228.

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The blood volume cultured in the detection of bacteraemia is a major variable in treating patients with systemic inflammatory response syndrome. The fact that drawing optimal volumes (8–10 mL) of blood for culture increases the sensitivity of the method is well established. This study aimed to optimise the mean blood volumes (mBVs) to that recommended level in a small rural hospital by implementing a continuous quality improvement programme in clinical microbiology. The education of phlebotomists, followed by monthly feedback and coaching sessions, can influence the blood volume drawn by phlebotomists and improve the sensitivity of blood cultures. Statistically significant increase (p<0.001) in both mBVs and median blood culture volumes occurred within 5 months compared with the baseline values obtained in the preceding 10 months. This quality improvement was sustained over 1 year. The mBVs inoculated into aerobic culture bottles met the manufacturer’s instructions of a fill volume of 8 to 10 mL of blood per bottle and optimised the yield of isolation of organisms from blood cultures.
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Simner, Patricia J., Kelly A. Doerr, Lory K. Steinmetz, and Nancy L. Wengenack. "Mycobacterium and Aerobic Actinomycete Culture: Are Two Medium Types and Extended Incubation Times Necessary?" Journal of Clinical Microbiology 54, no. 4 (February 10, 2016): 1089–93. http://dx.doi.org/10.1128/jcm.02838-15.

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Mycobacterial cultures are historically performed using a liquid medium and a solid agar medium with an incubation period of up to 60 days. We performed a retrospective analysis of 21,494 mycobacterial and aerobic actinomycetes cultures performed over 10 months to determine whether two medium types remain necessary and to investigate whether culture incubation length can be shortened. Specimens were cultured using Bactec MGIT liquid medium and Middlebrook 7H11/S7H11 solid medium with incubation periods of 42 and 60 days, respectively. Time-to-positivity and the identity of isolates recovered from each medium were evaluated. A total of 1,205/21,494 cultures (6%) were positive on at least one medium. Of the 1,353 isolates recovered, 1,110 (82%) were nontuberculous mycobacteria, 145 (11%) were aerobic actinomycetes, and 98 (7%) wereMycobacterium tuberculosiscomplex. Assessing medium types, 1,121 isolates were recovered from solid medium cultures, 922 isolates were recovered from liquid medium cultures, and 690 isolates were recovered on both media. Liquid cultures were positive an average of 10 days before solid cultures when the two medium types were positive (P< 0.0001). Isolates detected on solid medium after 6 weeks of incubation included 65 (5%) nontuberculous mycobacteria, 4 (0.3%) aerobic actinomycetes, and 2 (0.2%) isolates from theM. tuberculosiscomplex. Medical chart review suggested that most of these later-growing isolates were insignificant, as the diagnosis was already known, or they were considered colonizers/contaminants. This study reaffirms the need for both liquid medium and solid medium for mycobacterial and aerobic actinomycetes culture and demonstrates that solid medium incubation times may be reduced to 6 weeks without significantly impacting sensitivity.
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SCHIEMANN, DONALD A. "Examination of Enterotoxin Production at Low Temperature by Yersinia spp. in Culture Media and Foods." Journal of Food Protection 51, no. 7 (July 1, 1988): 571–73. http://dx.doi.org/10.4315/0362-028x-51.7.571.

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Sixteen of 27 (59%) cultures of Yersinia spp. produced enterotoxin measured by the suckling mouse assay at 25°C in aerated broth culture media. Only one of 15 of these cultures, which was identified as Yersinia kristensenii, produced enterotoxin at 6°C. No enterotoxin was detected in water and methanol extracts of 6 food slurries inoculated with this toxigenic culture after incubation at 9.8°C for 4 d.
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Balaji, S., A. Sujatha, and I. Kalyanasundaram. "A simple rapid procedure for obtaining axenic cultures from monoxenic cultures of myxomycete plasmodia." Canadian Journal of Microbiology 45, no. 10 (October 1, 1999): 865–70. http://dx.doi.org/10.1139/w99-080.

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Axenic culture of myxomycete plasmodia has been attempted from time to time by various authors, but with very little success. From over 500 known species of myxomycetes, fewer than 20 species have been reported in axenic culture to date, including axenic myxamoebal cultures. In these cultures, the plasmodia required either complex media, or a killed bacterial supplement for growth. Furthermore, the time required for attaining the axenic state varied from several months to years. In the present study, a simple, rapid procedure has been developed to render monoxenic plasmodial cultures axenic. This procedure is based on our discovery that plasmodia have certain unusual substrate preferences that are inhibitory to the associated bacteria using Physarella oblonga as a model. The presence or absence of the bacteria could be ascertained through incubation in four different bacteriological media and by the use of a differential staining technique.Key words: myxomycetes, axenic culture, hydrocarbon utilization, bacterial associates.
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Traynor, Peter. "Culture Media SIG." Microbiology Australia 33, no. 4 (2012): 000. http://dx.doi.org/10.1071/ma12903.

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The Culture Media Special Interest Group of the Australian Society for Microbiology was formed in 1991 by a group of interested individuals after an upsurge in interest in the issue of media quality and the appearance that no common standards or consensus existed in this area in Australia. Increased interest, especially amongst medical microbiologists, in what was being done, or should be done, by way of assuring the quality of microbiological media made the issue contentious.
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Huang, Felicia Scaggs, Andrea Ankrum, Cincinnati Hospital, Zheyi Teoh, Joshua Courter, Francesco, Mangano, Karin Bierbrauer, and Josh. "Learnings from a Cutibacterium acnes pseudo-outbreak in pediatric neurosurgical patients." Antimicrobial Stewardship & Healthcare Epidemiology 2, S1 (May 16, 2022): s57—s58. http://dx.doi.org/10.1017/ash.2022.166.

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Background:Cutibacterium acnes is normal skin flora as well as a common culture contaminant. It can cause infections in the setting of sterile implants, although clinical presentations can be subtle. Differentiating true infection from sample contamination is challenging and has implications for patient care. We describe an investigation of a cluster of 7 hospitalized pediatric patients with C. acnes isolated from anaerobic cultures of cerebrospinal fluid (CSF) over 3 weeks at a quaternary-care children’s hospital. Methods: An outbreak response was coordinated between the infection prevention and control (IPC), microbiology, and neurosurgery teams. We defined a case as a hospitalized patient with C. acnes isolated from a CSF culture beginning in November 2020. We reviewed charts of all cases and CSF culture collection on all case units, transport to and processing at the microbiology laboratory, and the IPC team measured adherence for all processes. Results: There were 8 positive cultures in 7 cases from November 10 to 27, 2020. The median case age was 2 months (range, 0–119). Cases occurred on 4 different units. All positive patients had at least 1 implanted neurosurgical device used for CSF drainage. There were no clear commonalities in surgeon responsible for device placement, hardware type placed, or staff collecting CSF samples. A standard protocol for CSF collection was followed for all cases. Overall, 3 patients cleared cultures without intervention, 2 received oral antibiotics, and 2 underwent surgical removal of their device. Specimen processing was unchanged, although due to supply issues, an alternative anaerobic culture media (Anaerobic Systems, Morgan Hills, CA) was used for 6 weeks, during which all cases were identified. Compared to routine media, the alternative is known to enhance organism detection. The company reported no concerns for media contamination or C. acnes outbreaks. Once routine media became available, CSF culture positivity for C. acnes returned to baseline (late November or early December) (Fig. 1). Conclusions: We identified a likely pseudo-outbreak related to temporary use of a more sensitive culture media. No direct patient harm was identified, although many had increased risk of harm by surgical intervention or prolonged length of stay. Technological advances may enhance organism identification but challenge existing paradigms of care. More studies are needed to better delineate the intersection of diagnostic advancements with patient care standards.Funding: NoneDisclosures: None
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Dissertations / Theses on the topic "Microbiology – Cultures and culture media"

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Lopes, Isabella de Cenço [UNESP]. "Produção de conídios do fungo entomopatogênico Metarhizium anisopliae em diferentes condições de cultivo e em biorreator de bandeja." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/139445.

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O objetivo deste trabalho foi de aumentar a produção de conídios de Metarhizium anisopliae através de alterações do substrato e das condições de cultivo e produzi-lo em biorreator de bandeja. Os substratos testados foram arroz tipo 1, quirera de arroz e farelo de arroz, sendo o cultivo realizado em embalagens plásticas contendo 10 g de substrato seco. Inicialmente, foi empregado arroz tipo 1 como substrato, variando-se as formas de umidificação no seu preparo, sendo o cultivo realizado em embalagens plásticas contendo 10g de substrato seco. Determinada a condição adequada de umidificação, os substratos arroz tipo 1 e quirera de arroz foram submetidos a diferentes condições de fotoperíodo: escuto contínuo, claro contínuo e escuro e claro alternados. O farelo de arroz foi adicionado a bagaço de cana-de-açúcar de modo a estruturar fisicamente o meio de cultivo. Os melhores resultados foram obtidos com arroz e quirera de arroz. A primeira alternativa de ampliação de escala foi realizada em embalagens plásticas, utilizando arroz tipo 1 e quirera com 500g de substrato seco. A etapa seguinte da ampliação de escala foi em um biorreator de bandeja com aeração realizada sobre a camada de substrato, sendo os testes realizados com arroz tipo 1, e duas espessuras de camada partículas, 2 e 4 cm. Em todos os ensaios, a resposta observada foi a concentração final de conídios. Foi testada ainda a virulência dos conídios produzidos em relação a lagartas de Diatraea flavipennella nas diferentes condições de produção. De acordo com os resultados apresentados, o farelo de arroz não é uma boa opção de substrato devido a sua pouca praticidade de manipulação e a quirera apresentou resultados satisfatórios nos ensaios, podendo ser considerada uma opção viável para utilização industrial devido ao seu baixo custo. O biorreator de bandeja elaborado apresentou bons resultados de produção de conídios em relação a produção em embalagem plástica de maior capacidade com o substrato arroz tipo 1, a qual, no entanto, apresentou produção de esporos inferior à da embalagem de menor capacidade. Os testes de virulência comprovaram a eficiência dos conídios em todos os ensaios com pequenas variações no tempo de mortalidade.
The work targeted the increase of the production of spores of Metarhizium anisopliae through modifications of the substrate and of the cultivation conditions, and produce such spores in a tray bioreactor. Type 1 rice, broken rice and rice bran were tested as substrate, which were cultivated in plastic bags containing 10 g of dry substrate. Alternatives of humidification were tested with type 1 rice. For the best alternative of humidification, type 1 rice and broken rice were submitted to alternatives of different exposures to light, provided by a fluorescent lamp: continuous dark, continuous light, alternation between dark and light. To the bran was added sugar cane bagasse in order to provide physical structure to the culture medium. The best results were obtained with rice and broken rice and the illumination regime does not influence on the spore production. The first attempt of scaling-up the spore production was the use of plastic bags containing 500 g of dry substrate, either rice or broken rice. The next scaling-up step was in a tray bioreactor aerated flowing air parallel to the top of the cultivation medium, using type 1 rice as substrate in two different thickness of medium, 2 and 4 cm. In every experiments, the response variable was the conidia concentration. The virulence against Diatraea flavipennella caterpillars was also tested for spores produced in the different experimental conditions. The results showed that rice bran is not an efficient alternative due to its difficult manipulation, while broken rice produce conidia concentration similar to type 1 rice and can be considered a cheap alternative for industrial production of spores. The tray bioreactor presented similar results to the large capacity plastic bags, which presented lower conidia concentrations than the small capacity container. The virulence experiments showed high efficiency of the conidia in all tested samples, with little variation in the time of lethality.
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Bateman, Colin Neil. "Investigating gene induction in Listeria monocytogenes during growth in complex media or tissue culture." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/3619/.

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Ortmanis, Andris. "The development of a microcomputer controlled variable pathlength turbidimeter /." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65460.

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Costa, André Luis da 1982. "Corte normalizado em grafos = um algoritmo aglomerativo para segmentação de imagens de colonias de bactérias= Normalized cut on graphs: an aglomerative algorithm for bacterial colonies image segmentation." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267753.

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Orientador: Marco Antonio Garcia de Carvalho
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Tecnologia
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Resumo: O problema de segmentação de colônias de bactérias em placas de Petri possui algumas características bem distintas daquelas encontradas, por exemplo, em problemas de segmentação de imagens naturais. A principal característica é o alto número de colônias que podem ser encontradas em uma placa. Desta forma, é primordial que o algoritmo de segmentação seja capaz de realizar a segmentação da imagem em um grande número de regiões. Este cenário extremo é ideal para analisar limitações dos algoritmos de segmentação. De fato, neste trabalho foi verificado que o algoritmo de corte normalizado original, que se fundamenta na teoria espectral de grafos, é inadequado para aplicações que exigem que a segmentação seja realizada em um grande número de regiões. Contudo, a utilização do critério de corte normalizado para segmentar imagens de colônias de bactérias ainda é possível graças a um novo algoritmo que está sendo introduzido neste trabalho. O novo algoritmo fundamenta-se no agrupamento hierárquico dos nós do grafo, ao invés de utilizar conceito da teoria espectral. Experimentos mostram também que o biparticionamento de um grafo pelo novo algoritmo apresenta um valor de corte normalizado médio cerca de 40 vezes menor que o biparticionamento pelo algoritmo baseado na teoria espectral
Abstract: The problem of bacteria colonies segmentation in Petri dishes has some very different characteristics from those found, for example, in segmenting natural images. The main feature is the high number of colonies that can be found on a plate. Thus, it is essential that the segmentation algorithm is capable of performing the image segmentation into a huge number of regions. This extreme scenario is ideal for analyzing segmentation algorithms limitations. In fact, this study showed that the original normalized cut algorithm, which is based on the spectral graph theory, is inappropriate for applications that require that the segmentation be performed on a large number of regions. However, the use of normalized cut criteria for segmenting bacteria colonies images is still possible thanks to a new algorithm that is being introduced in this paper. The new algorithm is based on hierarchical clustering of the graph nodes, instead of using the spectral theory concepts. Experiments also show that the bi-partitioning of a graph by the new algorithm has a normalized cut average value about 40 times lesser than the bi-partitioning by the algorithm based on the spectral theory
Mestrado
Tecnologia e Inovação
Mestre em Tecnologia
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Bombardi, Franciele Mendes de Lima. "Sensoriamento ótico da dinâmica do crescimento de colônias de escherichia coli em ambiente hídrico." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2526.

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Este trabalho apresenta um estudo empregando duas técnicas óticas para monitorar o crescimento de culturas de cepas de Escherichia coli em dois meios de cultura líquidos: Espectroscopia de absorção UV-Vis (turbidimetria) e espectroscopia Raman. Na primeira técnica, a turbidez permite avaliar as diferentes fases naturais de crescimento de uma cultura bacteriana (lag, exponencial, estacionária e decaimento) por meio da densidade ótica, medida com um espectrômetro UV-VIS. Na segunda, o espalhamento Raman (medido com um espectrômetro dispersivo), a partir de amostras de água contaminada, fornece não apenas informações sobre as fases de crescimento, mas também abre a possibilidade de identificação bacteriana através da sua impressão digital característica. Mediu-se a dinâmica de duas cepas de E. coli – (nomeadas como H2/11 e H3C2/12) em um caldo líquido nutriente e quatro cepas de E. coli (nomeadas como H2/11, H3C2/12, 109 e 110) em caldo líquido EC, mantidas a 37,0°C ao longo de 24 horas. Alíquotas das amostras foram removidas da cultura em intervalos de tempo regulares para medições espectrais. A análise da turbidez permitiu medir o tempo de geração (isto é, o tempo de duplicação de uma população), que foi maior para cepas crescidas em caldo EC. Os espectros Raman forneceram informações sobre a evolução temporal das bandas a 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associadas com impressões digitais de componentes biológicos específicos. Os dados espectrais foram analisados por Análise de Componentes Principais (PCA). Os resultados obtidos em ambas as técnicas permitiram identificar as fases lag, exponencial e estacionária das cepas estudadas.
This work is a study using two optical techniques to monitor the growth of cultures of Escherichia coli strains in two liquid culture medium: Raman spectroscopy and UV-Vis absorption spectroscopy (turbidimetry). In one hand, turbidity allows evaluating the different phases of growth of a bacterial culture (lag, exponential, stationary and decay) by optical density, measured with an UV-VIS spectrometer. On the other hand, Raman scattering (measured with a dispersive spectrometer) from contaminated water samples not only provides information about the grow phases, but also opens a possible identification of bacterial by its characteristic fingerprint. Two strains of E. coli (named as H2 / 11 and H3C2 / 12) were measured in liquid nutrient broth and four E. coli strains (named H2 / 11, H3C2 / 12, 109 and 110) in EC liquid broth, kept at 37.0 ° C over 24 hours. Aliquots of the samples were removed from the culture at regular time intervals for spectral measurements. The turbidity analysis allowed to measure the generation time, which was higher for strains grown in EC broth. Raman spectra provided information about the time evolution of the bands at 942 cm-1, 977 cm-1, 1036 cm-1, 1086 cm-1, 1140 cm-1, 1188 cm-1, 1182 cm-1, 1207 cm-1 e 1251 cm-1, associated with fingerprints of biological components. Data were analyzed by Principal Component Analysis (PCA). These results of both techniques allowed identifying the phases lag, exponential and stationary of the studied strains.
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Elhaneid, Mohamed. "Toxicological assessment of graphene based nanomaterials in cell culture models." Thesis, University of Birmingham, 2019. http://etheses.bham.ac.uk//id/eprint/8881/.

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Graphene oxide (GO) and reduced GO (r-GO) nanomaterials exhibit great potential for several biomedical applications. Of foremost importance is to determine any potential health hazards related in their exposure. In this research, we hypothesised that the different material properties evidenced by GO and r-GO would elicit different biological responses. The first objective of this work was to synthesize Go and r-GO and characterize their physiochemical properties. The second aim was to investigate whether the two-distinct surface chemistries of GO and r-GO influenced their biological effect. The potential toxicity of these nanomaterials was investigated using the normal lung fibroplast cell line MRC-5 and cancerous epithelial lung cell line A549. The cytotoxicity of graphene derivatives was concentration-, time- and cell-dependent and varied according to the material used. Thus, the surface chemistry of graphene plays a critical role in its biocompatibility. Non-cancerous cells had a higher sensitivity to GO cytotoxicity than cancer cells. R-GO was highly biocompatible to MRC-5 cells and for A549 cells had a minimal effect of cell viability. At 37C˚, GO and r-GO were moderately hemolyric at concentration of 125 µg/ml and highly haemolytic at concentration of 300 µg/ml. Exposure of cells to both graphene derivatives led to reactive oxygen species (RO5) generation without genotoxicity. GO, but not r-GO, led to autophagy in both cell lines, possibly inhibiting the PIP3-Akt/mTOR pathway. For both cell lines and at non-lethal concentrations, GO downregulated the expression of glycogen synthase kinase-3 (GSK-3ß). GO was also found to dysregulate both Wnt/b-catenin and Akt cell signalling pathways which are vital for cellular function. The finding relating to cell signalling provide an insight to the safety of GO which is important to its use in cancer therapy.
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Pickell, Lisa D. "Investigating the Effects of Organic Ligands on Iron and Copper Availability to Coastal and Oceanic Phytoplankton Using Continuous Cultures." Fogler Library, University of Maine, 2008. http://www.library.umaine.edu/theses/pdf/PickellLD2008.pdf.

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Thornton, Sarah. "Record hops to raves : authenticity and subcultural capital in music and media cultures." Thesis, University of Strathclyde, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261836.

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Tsoulis-Reay, Alexa. "Convergence, concern & the "real" girl : teenage girls' everyday media cultures /." Connect to thesis, 2009. http://repository.unimelb.edu.au/10187/4893.

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Rivas, Cecilia Maribel. "Imaginaries of transnationalism media and cultures of consumption in El Salvador /." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2007. http://wwwlib.umi.com/cr/ucsd/fullcit?p3258783.

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Thesis (Ph. D.)--University of California, San Diego, 2007.
Title from first page of PDF file (viewed June 8, 2007). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 159-168).
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Books on the topic "Microbiology – Cultures and culture media"

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C, Parks Lawrence, ed. Handbook of microbiological media. 2nd ed. Boca Raton: CRC Press, 1997.

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C, Parks Lawrence, ed. Handbook of microbiological media. Boca Raton: CRC Press, 1993.

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Digestive Ferment Company. Difco Laboratories, Detroit. Difco manual: Dehydrated culture media and reagents for microbiology. Detroit: DIFCO Laboratories, 1994.

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Atlas, Ronald M. Handbook of media for environmental microbiology. Boca Raton: CRC Press, 1995.

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Handbook of media for environmental microbiology. 2nd ed. Boca Raton: Taylor & Francis, 2005.

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Atlas, Ronald M. Handbook of microbiological media. 4th ed. Boca Raton: Taylor & Francis, 2010.

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W, Snyder James, ed. Handbook of media for clinical microbiology. 2nd ed. Boca Raton: CRC Press, 2006.

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W, Snyder James, ed. Handbook of media for clinical microbiology. Boca Raton: CRC Press, 1995.

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R, Norris J., and Pettipher G. L. 1951-, eds. Essays in agricultural and food microbiology. Chichester [West Sussex]: J. Wiley, 1987.

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Hackett, Kevin James. Cultivation and detection of mycoplasmalike organisms: Final report, July 1, 1991. Beltsville, Md.?]: BARC-W, 1991.

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Book chapters on the topic "Microbiology – Cultures and culture media"

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Ginocchio, Christine C., Gerald Van Horn, and Patricia Harris. "Reagents, Stains, Media, and Cell Cultures: Virology." In Manual of Clinical Microbiology, 1422–31. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555817381.ch80.

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SenGupta, Manideepa, and Mallika Sengupta. "Culture media." In Practicals in Microbiology, 23. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12695_4.

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Kumar, Surinder. "Culture Media." In Essentials of Microbiology, 37. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12697_7.

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Kumar, Surinder. "Culture Media." In Textbook of Microbiology, 55. Jaypee Brothers Medical Publishers (P) Ltd., 2012. http://dx.doi.org/10.5005/jp/books/11668_6.

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Sandle, Tim. "Microbiological culture media." In Pharmaceutical Microbiology, 47–61. Elsevier, 2016. http://dx.doi.org/10.1016/b978-0-08-100022-9.00005-0.

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Selvaraj, Logeswari. "Culture Media." In Textbook of Microbiology for Paramedicals, 24. Jaypee Brothers Medical Publishers (P) Ltd., 2008. http://dx.doi.org/10.5005/jp/books/11041_5.

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Kumar, Surinder. "Culture Media." In Practical Microbiology for MBBS Students, 15. Jaypee Brothers Medical Publishers (P) Ltd., 2018. http://dx.doi.org/10.5005/jp/books/14195_5.

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Rao, RR. "Culture Media." In Textbook of Microbiology for Nursing Students, 26. Jaypee Brothers Medical Publishers (P) Ltd., 2014. http://dx.doi.org/10.5005/jp/books/12375_6.

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Anthikad, Jacob, and P. Sumanaswini. "Culture Media." In Medical Microbiology for Nurses (Including Parasitology), 21. Jaypee Brothers Medical Publishers (P) Ltd., 2013. http://dx.doi.org/10.5005/jp/books/11930_6.

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Rao, RR. "Culture Media." In Textbook of Microbiology for Nursing Students, 28. Jaypee Brothers Medical Publishers (P) Ltd., 2005. http://dx.doi.org/10.5005/jp/books/10922_6.

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Conference papers on the topic "Microbiology – Cultures and culture media"

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Valle-Algarra, F. M., Eva M. Mateo, M. A. García-Esparza, R. Mateo, J. V. Gimeno-Adelantado, and M. Jiménez. "Production of patulin by Penicillium expansum in different culture media including bee pollen media." In Proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009). WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789814322119_0092.

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Ren, Nanqi, Yongfeng Li, Maryam Zadsar, Lijie Hu, and Jianzheng Li. "Biological Hydrogen Production In China: Past, Present and Future." In ASME 2005 International Solar Energy Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/isec2005-76101.

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As a new clean energy source and important material, the use and demand of hydrogen are increasing-rapidly. So that bio-hydrogen producing technology moves toward cutting down the operation costs in recent years. Biohydrogen production capacity improvement and cost reduction are two key points for industrialization of the process. Biohydrogen production has been studied in China for over 20 years in both photosynthetic hydrogen production and fermentative processes fields. The anaerobic process of fermentative hydrogen production has been developing in China since 1990s. The isolation and identification of high efficient bio-hydrogen production anaerobic bacteria is an important foundation of fermentative bio-hydrogen production process by anaerobic digestion of organic wastewater. The paper focuses on: (1) Fermentative biohydrogen production system, (2) Laboratory experiments and pilot scale tests for continued hydrogen production, (3) Fermentation types and their engineering control, (4) isolation, culture media and characterization of anaerobes, (5) Applications of pure bacteria, (6) Fundamental researches including ecology, genetics and improvements, (7) Development of two-phase anaerobic process of H2-producing and methanogenic phases as, and (8) the integrated processes with bioengineering and wastewater treatments. Recently, the first pilot factory has been costructedin Harbin, China by hydrogen production rate of more than 1200m3/d which located in northeast of China. In photosynthetic hydrogen production filed, study is focused on the fundamentals, engineering application and microbiology. Detailed discussion comes later.
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Telpov, Roman E. "Physical And Geographical Identity Features In Local Media Texts." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.104.

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Serebrennikova, Evgenia F. "Transnational Cognitive Units As Attractive Signs Of The Media Dialogue." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.93.

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Korotkevich, Daria O. "A Foreign Country As A Subject Of American Mass Media Discourse." In Dialogue of Cultures - Culture of Dialogue: from Conflicting to Understanding. European Publisher, 2020. http://dx.doi.org/10.15405/epsbs.2020.11.03.46.

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Mauk, Michael G., Carlos Ruiz, Richard Y. Chiou, Jean Espaillat, Senyu Wang, Ainhoa Garcia, and Robert Surrette. "Student Learning Projects in Sustainable Energy: Solar-Powered Algae Culture, Photovoltaics, and CO2 Capture." In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-88404.

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A systems approach combining algae aquaculture for biofuels, photovoltaic solar electric generation, and a subsystem for atmospheric CO2 capture as a carbon source for the algae, is developed as a platform and testbed for student engineering design projects. This work serves as a hands-on learning design project for engineering undergraduates in renewable energy, industrial microbiology, and sustainable and ‘green’ technology. Fully-instrumented and automated tabletop systems for circulating algae cultures in plastic fluidic channels integrated with silicon solar cells are designed, prototyped and tested with the aim of optimizing the yields and solar energy utilization.
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Canelas, Vitória Luciana Paiva, Alessandro Henrique Nascimento, Ana Júlia Vieira Da Silva Platilha, Bianca Pimentel Borges, Kássia Alany Girard Cardias, and William Franklim Da Silva Alves. "EXAMES MICROBIOLÓGICOS DE FELINO ACOMETIDO POR KLEBSIELLA SPP. NO HOSPITAL VETERINÁRIO MÁRIO DIAS TEIXEIRA (HOVET-UFRA)." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1875.

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Introdução: O diagnóstico de bactérias resistentes, como a Klebsiella spp., necessita da realização de exames laboratoriais. Essa bactéria, pertencente à família Enterobacteriaceae, é capaz de inibir a fagocitose por meio da produção de material capsular abundante, prolongando sua sobrevivência no ambiente intracelular. Objetivos: O objetivo deste trabalho foi realizar exames microbiológicos, como cultura bacteriana e antibiograma, a fim de identificar a presença de uma bactéria em uma amostra, registrando suas características morfológicas, microbiológicas e bioquímicas. Materiais e Métodos: O material foi coletado de um felino doméstico de um ano de idade no Hospital Veterinário Mário Dias Teixeira (HOVET-UFRA) em Belém, Pará, o paciente apresentava uma ferida cutânea sem remissão, com tempo de evolução de 5 meses, a lesão media 10 cm de comprimento por 4 cm de largura, com bordos delimitados, superfície ulcerada e serosanguinolenta. Foi solicitada cultura bacteriana e antibiograma da lesão. As amostras foram coletadas após a limpeza com solução fisiológica estéril, realizando-se a coleta por meio de escarificação com o auxílio de um swab estéril para análise no laboratório de microbiologia da universidade. Foram utilizadas técnicas laboratoriais para o cultivo microbiológico e para provas bioquímicas. Resultados: Como resultado foi detectado o crescimento de colônias do gênero Klebsiella spp. nos meios de Ágar Sangue ovino a 5% e Ágar MacConkey após 24 horas. Dentre as propriedades que permitem a identificação de Klebsiella spp. entre os gêneros de enterobactérias, estão as características morfológicas das colônias que são mucoides, não hemolíticas e fermentadoras de lactose. As provas bioquímicas apresentaram resultados de motilidade negativa, citrato positivo (utilização de citrato como fonte de carbono), lisina positiva, uréia negativa, H₂S negativo, tríplice açúcar ferro (TSI) com fermentação de glicose, lactose, sacarose e com produção de gás, concordando com a literatura descrita para Klebsiella spp. Na análise do antibiograma a bactéria apresentou sensibilidade à gentamicina, azitromicina, norfloxacina, ciprofloxacina, cefotaxima e amoxicilina com clavulanato e resistência à ampicilina e sulfametoxazol com trimetoprim. Conclusão: Concluímos que por meio da interpretação dinâmica dos testes microbiológicos, bioquímicos e antibiograma foi possível identificar a Klebsiella spp. na amostra, demonstrando a eficácia dos testes na detecção de infecções bacterianas.
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Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

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Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. In particular, when culturing cells within hydrogel scaffold-filled networks, as may be done in micro-tissue engineering, the need for perfusion culture also increases the likelihood of a destructive bubble entering the network. Moreover, analysis of micro-cultures frequently entails labelling with antibodies and/or fluorescent probes, which again requires controlled perfusion of the various reagents through the network. We have developed a simple technique to preserve cell viability and simplify labeling within microscale cultures without the need for perfusion. Instead of bonding a microfluidic network to glass, PDMS, or another impermeable substrate, the network is bonded to a semi-permeable microdialysis membrane, which allows free exchange of oxygen, proteins, nutrients, and waste between the microfluidic channels and culture media in static culture plates.
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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarrier to microcarrier culture can be achieved with careful management of culture conditions and brief exposure to enzymes.Human umbilical artery and vein, and saphenous vein endothelial cells were prepared and grogn on microcarrier cultures to cell populations of 200-450 x 106 cells and conditioned for 14 day periods in serum-free media.The production profiles of several endothelial cell proteins including thrombospondin (TSP), von Willebrand Factor (vWF) and issue plasminogen activator (t-PA) were measured by radioimmunoassay under these conditions, and demonstrate the use of microcarrier cultures in producing milligram quantities of engothelial cell protein. For example, a HUVEC culture of 200 x 106 cells conditioned with serum-free media for 14 days yielded a total of 6.9mg TSP, 0.7mg vWF and 48.9ug t-PA. In this laboratory one such application of the system was the purification of endothelial proteins in amounts sufficient for immunisation of mice prior to the production of monoclonal antibodies and for subsequent characterisation.
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Merryman, W. David, Kristen Loveless, and Mehran Kasra. "Disc Nucleus Cellular Response to Dynamic Pressures at Critical Frequencies: A Pig Model." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-43092.

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Disc degeneration is a multifactor phenomenon. It has been found that intervertebral disc (IVD) cells respond to such factors as pH, osmotic pressure, genetic factors, and mechanical loading (Guilak, 1999). During daily activities the human intervertebral disc is exposed to oscillatory hydrostatic loads that produce pressures &gt;2MPa in vivo (Nachemson, 1964 and 1979). It is known that dynamic loads with critical frequencies close to that of the in vivo human spine resonant frequency (4–5 Hz) have a destructive effect on disc tissue properties (Pope, 1993). Whether this destructive effect is purely mechanical, due to loading magnification, or biological, affecting cell metabolism, is unknown. Previous work (Merryman, 2002) showed that there was no significant effect upon monolayer IVD cells loaded at 15Hz, while lower frequencies (1 and 8Hz) altered collagen synthesis compared to control. To address this issue, we developed a mechanically active culture system capable of delivering a wide range of loading frequencies and amplitudes of hydrostatic pressure to cultures of disc cells. Nucleus pulposus cells of pig discs were isolated and suspended in alginate beads. Alginate cultures were divided into 6 groups; five groups were exposed to cyclic pressures of frequencies 1, 3, 5, 8, and 10Hz with the same amplitude of 1MPa, and group 6 was the control group (no loading). Cultures of different groups were loaded for 3 days (30 minutes daily) in a hydraulic chamber filled with culture media. The effect of loading frequency on collagen metabolism among different groups was compared by measuring incorporated [3H]-proline into collagen for medium and total extracts. The results indicated a poor synthesis rate and more degradation near the 5Hz frequency.
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Reports on the topic "Microbiology – Cultures and culture media"

1

Hrytsenko, Olena. Sociocultural and informational and communication transformations of a new type of society (problems of preserving national identity and national media space). Ivan Franko National University of Lviv, February 2022. http://dx.doi.org/10.30970/vjo.2022.51.11406.

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The problems of the correlation of cosmopolitan and national identities are too complex to be unambiguous assessment, let alone alternative values (related to the ecological paradigm and the spiritual traditions of other cultures). However, it is obvious that without preserving the national identity, the integrity and independence of the national state becomes problematic. On the other hand, without taking into account the consequences of information wars and aggressive cosmopolitan tendencies of global media culture, there is a threat of losing the national information space and displacing it to the periphery of socio-political and economic life in Ukraine and in the modern world. In the process of working on research issues, the author of the article came out on the principles of objectivity, systematic and determinism, which in combination of their observance made it possible to determine the influence of the post-industrial information society on the formation of a new type of mass consciousness. As a result of the influence of globalization processes, there was a filling of the domestic information space with a supernational mass culture of entertainment, which in most cases leads to the spread of a primitive world outlook based on the ideology of consumption society, without leaving places to preserve sociocultural traditions and national identity. Therefore, given the problems of preserving national identity, it is necessary should be mentioned the information security of the state, which occupies one of the most important places, among various aspects of information security, since the unresolved problem of protection of the national information space significantly complicates the processes of formation of national identity.
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