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Journal articles on the topic 'Microclones'

Author: Grafiati

Published: 30 May 2022

Last updated: 31 July 2025

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1

Marecki, Wojciech, and Jadwiga Żebrowska. "In Vitro Pathogenesis Caused by Phytophthora cactorum and DNA Analysis of the Strawberry-Resistant Microplants with ISSR Markers." Agronomy 11, no. 7 (2021): 1279. http://dx.doi.org/10.3390/agronomy11071279.

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The soil pathogenic fungus Phytophthora cactorum causes the most dangerous diseases occurring in strawberry plantations—strawberry crown rot and leather rot. Modern biotechnology methods, e.g., in vitro culture selection and molecular diagnostics can be utilized in the selection of cultivars that are less susceptible or resistant to Phytophthora diseases. In this study, in vitro selection of four strawberry microclones: ‘Elsanta’, ‘Feltar’, ‘Teresa’ and ‘Plena SVdT’ against Phytophthora cactorum (Lebert and Cohn) J. Schröt was carried out. Molecular analysis with inter simple sequence repeat (

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2

Yalouskaya, N. A., J. N. Kalatskaja, N. A. Laman, V. V. Nikalaichuk, A. N. Kraskouski, and K. S. Hileuskaya. "Caffeic Acid in Various Formulations as a Growth and Resistance Regulator of Potato Microclones in <i>in vitro</i> Culture." Прикладная биохимия и микробиология 59, no. 5 (2023): 502–11. http://dx.doi.org/10.31857/s0555109923050045.

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The article discusses the influence of caffeic acid (CA), its mix with chitosan (CHT + CA) and chitosan-based conjugate (CHT-CA) on growth and proline content of microclone potato plants (Solanum tuberosum L.) in in vitro culture under optimal conditions and under prolonged osmotic stress caused by polyethylene glycol. Under optimal conditions CHT-CA and CA, acting as moderate strength stressors, accelerate the growth and development of potato microclones and increase the proline accumulation in the stems. Under osmotic stress CA and CHT-CA promote the resistance of potato microclones and main

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3

Kirgizova, I. V., E. A. Kalashnikova, R. M. Turpanova, A. M. Gadzhimuradova, and D. V. Silaev. "Levels of physiological activity of antioxidant enzymes in potatoes (S. tuberosum L.) when infected PVS virus." IOP Conference Series: Earth and Environmental Science 1154, no. 1 (2023): 012033. http://dx.doi.org/10.1088/1755-1315/1154/1/012033.

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Abstract This article presents the results of changes in the activity of potato antioxidant enzymes during infection with the PVS virus. As a result of the research, it was found that the formation of callus to obtain microclones is more efficient when using leaf explants, compared with stem explants in all studied varieties. The article presents that varieties with different susceptibility have different enzymatic activity, which can serve as a marker trait when ranking potatoes in breeding. Interestingly, in potato microclones, the activity of POX, CAT and SOD upon infection was significantl

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Olson, Paul, Razif Gabdoulline, Rabia Shahswar, et al. "Prognostic Impact of Subclinical FLT3-ITD Microclones in Patients with Acute Myeloid Leukemia." Blood 144, Supplement 1 (2024): 847. https://doi.org/10.1182/blood-2024-207703.

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Background Patients with acute myeloid leukemia (AML) without FLT3 internal tandem duplication (ITD) at diagnosis often relapse with FLT3-ITD. Therefore, we evaluated the frequency of FLT3-ITD microclones in FLT3-ITD negative patients to evaluate the prognostic impact of subclinical FLT3-ITD microclones in a large cohort of AML patients. Methods Patients with AML who underwent allogeneic hematopoietic cell transplantation (alloHCT) at Hannover Medical School between 2000 and 2023 were included, if FLT3-ITD was negative by fragment length analysis at diagnosis (FLT3-ITD mutant to wildtype ratio

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Yalouskaya, N. A., J. N. Kalatskaja, N. A. Laman, K. S. Hileuskaya, A. N. Kraskouski, and V. I. Kulikouskaya. "Influence of chitosan-stabilized silver nanoparticles on the physiological and biochemical state of potato plants (<i>Solanum tuberosum</i> L.) <i>in vitro</i> culture." Proceedings of the National Academy of Sciences of Belarus, Biological Series 68, no. 1 (2023): 15–26. http://dx.doi.org/10.29235/1029-8940-2023-68-1-15-26.

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The article contains the results of study of the influence of added to culture medium silver-containing chitosan- based nanocomposites (Chitosan-Ag) at a dilution of 1:500 and 1:1000 (the mass ratio of the components is 50:1 for Chit- Ag 50:1 and 100:1 for Chit-Ag 100:1) on the development of potato microshoots and microclones with a formed root system. Potato microshoots cultivated for 4 weeks on nutrient medium modified with nanocomposites were characterized by slow development and the absence of rhizogenesis, which indicates the toxic effect of the studied nanocomposite concentrations. When

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Kirakosyan, R. N., Ya Yu Golivanov, V. I. Trukhachev, et al. "Colleter Formation on the Leaves of Sweet Potato Microclones (Ipomoea batatas L.) under In Vitro Conditions." Физиология растений 70, no. 6 (2023): 612–22. http://dx.doi.org/10.31857/s0015330323600201.

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Microclones of in vitro vegetable sweet potato (Ipomoea batatas L.) were received and the formation of colors on their leaves was studied. The studies were carried out on three varieties of sweet potato that differ in the color of their pulp and the peel of the tubers as well as in different ripening periods: Purple, Jewel, and Muskatny. The effect of ½ norm of mineral salts MS medium, 1 norm MS and 1½ norm MS on the morphometric parameters of sweet potato microclones was studied. It was established that the use of ½ norm of mineral salts according to MS medium led to the production of rapidly

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Turganova, R. A., E. D. Djangalina, E. A. Shadenova, A. I. Kapytina, and G. K. Kamshybayeva. "FEATURES OF THE INTRODUCTION INTO IN VITRO CULTURE AND MICROPROPAGATION OF PAULOWNIA TOMENTOSA." REPORTS 2, no. 336 (2021): 18–25. http://dx.doi.org/10.32014/2021.2518-1483.25.

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Paulownia sp. are tall and fast-growing perennial plants that grow faster than all woody plants in the world. In many countries, Paulownia sp. are used as a raw material in bioenergy, furniture industry, landscape gardening and technologies for phytoremediation. In this study for the first time in Kazakhstan, conditions of Paulownia tomentosa (P. tomentosa) in vitro cultivation and propagation have been optimized, also the factors influencing the morphogenetic activity of primary explants have been studied. Along with the adaptation potential of Paulownia tomentosa microclones to the ex situ c

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8

Kravets, N. B., N. V. Tulaidan, M. Z. Mosula, and N. M. Drobyk. "Microclonal propagation and callus induction of some species of Carlina L. genus." Faktori eksperimental'noi evolucii organizmiv 22 (September 9, 2018): 274–81. http://dx.doi.org/10.7124/feeo.v22.961.

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Aim. The aim of the research was to choose the conditions for microclonal propagation and obtain callus cultures from Carlina аcaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl plants in vitro. Methods. For microclonal propagation of С. acaulis, C. cirsioides and C. onopordіfolia we used rosettes of 2–3-month specimens and planted them on semi-solid Murashige and Skoog (MS) medium with decreased macro- and microsalts concentrations (MS/2) supplemented with kinetin (Кin) (from 1–3 mg/l) and 0.1 mg/l of 1-naphthaleneacetic acid (NAA). For induction of

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Anokhina, Nadejda S., Nuriya A. Urazbakhtina, and Ilgiz G. Asylbaev. "ADAPTATION OF MICROCLONES OF TRIPLOID ASPEN (POPULUS TREMULA L.) TO EX VITRO CONDITIONS." VESTNIK OF THE BASHKIR STATE AGRARIAN UNIVERSITY 71, no. 3 (2024): 6–11. http://dx.doi.org/10.31563/1684-7628-2024-71-3-6-11.

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As a result of deforestation, annual forest fires and increased emissions from industrial facilities, greenhouse gases accumulate in the atmosphere, which affects global climate warming. In these conditions, reforestation and forest protection measures play a special role. It is possible to obtain a sufficient amount of planting material for reforestation by introducing biotechnologies into forestry. The technology of microclonal reproduction makes it possible to obtain seedlings of tree crops that are difficult to propagate vegetatively. One of the most difficult stages of microclonal reprodu

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Tesliuk, N. I., O. Y. Zinchenko, M. B. Galkin, and T. O. Filipova. "IMPROVEMENT OF THE CHRYSANTHEMUM × KOREANUM HORT. CLONAL MICROPROPAGATION PROCESSES USING BACILLUS MEGATERIUM ONU500." Microbiology&Biotechnology, no. 2(58) (September 27, 2023): 17–29. http://dx.doi.org/10.18524/2307-4663.2023.2(58).287368.

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The aim of this study was to improve the Chrysanthemum × Koreanum micropropagation processes using Bacillus megaterium ONU500 at the post-aseptic adaptation stage. Methods. At the initial stages of clonal micropropagation, two surface sterilization protocols with different fungicidal preparations were tested. The influence of the agar concentration in the medium on the development of explants was determined. At the ex vitro adaptation stage, the roots of the microclones were inoculated with bacteria of the strain B. megaterium ONU500. Results. The most effective protocol for plant material sur

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Terletskaya, N. V., M. Yerbay, E. A. Shadenova, et al. "INFLUENCE OF LOW POSITIVE TEMPERATURES ON ANATOMICAL CHARACTERISTICS JUGLANS REGIA L. IN VITRO MICROCLONES." Eurasian Journal of Applied Biotechnology, no. 4 (December 28, 2023): 55–63. http://dx.doi.org/10.11134/btp.4.2023.6.

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Walnut (Juglans Regia L.) is a promising plant for use in modern medicine. We describe the reactions of Juglans Regia L. microclones exposed to cold stress in vitro at the cell and tissue level. Differences in the water content of plant tissues and the anatomical and morphological structure of the stem and leaf of Juglans Regia L. microclones under control and stress conditions are shown. Significant changes in the anatomical parameters of various stem tissues, both upward and downward, and a tendency for the values of the anatomical parameters of the leaf to decrease under cold stress conditi

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12

Terletskaya, Nina V., Elvira A. Shadenova, Yuliya A. Litvinenko, et al. "Influence of Cold Stress on Physiological and Phytochemical Characteristics and Secondary Metabolite Accumulation in Microclones of Juglans regia L." International Journal of Molecular Sciences 25, no. 9 (2024): 4991. http://dx.doi.org/10.3390/ijms25094991.

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The current study investigated the impact of cold stress on the morphological, physiological, and phytochemical properties of Juglans regia L. (J. regia) using in vitro microclone cultures. The study revealed significant stress-induced changes in the production of secondary antioxidant metabolites. According to gas chromatography–mass spectrometry (GC–MS) analyses, the stress conditions profoundly altered the metabolism of J. regia microclones. Although the overall spectrum of metabolites was reduced, the production of key secondary antioxidant metabolites significantly increased. Notably, the

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13

Kalashnikova, E. A., R. N. Kirakosyan, and A. V. Gushchin. "Application of aeroponic technologies for adaptation of microclones of berry crops to ex vitro conditions." Pomiculture and small fruits culture in Russia 80 (March 25, 2025): 37–47. https://doi.org/10.31676/2073-4948-2025-80-37-47.

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The transfer of plants from in vitro conditions to ex vitro conditions is a key stage in the process of clonal micropropagation. Based on our experiments and data from other researchers, it was found that when transferring microclones to non-sterile conditions, plants experience stress due to significant changes in their growing conditions. To minimize this stress, it is important to create conditions as close as possible to those in the artificial in vitro environment. One promising approach in this direction is aeroponic technologies, which were not previously used for these purposes. The co

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14

Tytarenko, N., N. Tesliuk, and V. Ivanytsia. "IMPACT OF ACTINOBACTERIA ON THE GROWTH AND ACCLIMATIZATION OF MICROPROPAGATED RUBUS FRUTICOSUS L. PLANTS TO EX VITRO CONDITIONS." Microbiology&Biotechnology, no. 1(57) (May 2, 2023): 18–41. http://dx.doi.org/10.18524/2307-4663.2023.1(57).276078.

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Microcloning is an effective method of plant reproduction that is actively developing in Ukraine for the mass propagation of valuable food crops such as blackberries. However, the problem of losing a large number of microclones often arises during the ex vitro acclimatization stage. Inoculation of the rhizosphere with potentially useful microorganisms could have a positive impact on the survival rate and biometric characteristics of acclimatized seedlings. The aim of this study was to establish the impact of 23 actinobacteria isolates on blackberry microclones (Rubus fruticosus L.) during accl

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Karakotov, S. D., I. V. Apasov, A. A. Nalbandyan, E. N. Vasilchenko, and T. P. Fedulova. "Modern issues of sugar beet (Beta vulgaris L.) hybrid breeding." Vavilov Journal of Genetics and Breeding 25, no. 4 (2021): 394–400. http://dx.doi.org/10.18699/vj21.043.

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High efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform starting material is proposed. It speeds up the breeding process two to threefold. The identification of haploid regenerants with sterile cytoplasm in initial populations is of great theoretical and practical importance for breeding, as it facilitates the production of homozygous lines with cytopla

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16

T.E., Strila. "Scientific grounds of microclones adaptation and cranberry growing in protected ground." Plant Introduction 9-10 (June 1, 2001): 139–45. https://doi.org/10.5281/zenodo.3334664.

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17

Mashkina, O. S., T. M. Tabatskaya, and O. M. Korchagin. "In vitro selection of birch for tolerance to salinity stress." IOP Conference Series: Earth and Environmental Science 875, no. 1 (2021): 012082. http://dx.doi.org/10.1088/1755-1315/875/1/012082.

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Abstract In vitro modelling of stress is one of the promising avenues for plant breeding for tolerance to negative environmental factors. In this study we examined the effect of NaCl (0.5%) on callusogenesis and morphogenesis of stem explants of different birch genotypes: Betula pendula Roth, B. pendula Roth var. carelica (Mercklin) Hämet-Ahti, B. pendula f. ‘dalecarlica’ (L.f.) Schneid., B. pubescens Ehrh. In our experiments we used pre-selected microclones from our in vitro collection on NaCl (0.2-1.0%) selective media. The clones were contrasted by the degree of their sensitivity to salinit

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18

Polishchuk, V. V., L. M. Karpuk, V. P. Mykolaiko, A. A. Polishchuk, and I. I. Mykolaiko. "In vitro rhizogenesis of sugar beet microclones." Regulatory Mechanisms in Biosystems 8, no. 4 (2017): 616–22. http://dx.doi.org/10.15421/021794.

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The features of rhizogenesis of male-sterile simple hybrids, maternal components of sugar beet heterozosis hybrid and O-types of Darinka variety grown from seeds were investigated. The paper presents the results of studying the main basic nutrient media (B5–A1, B5–A2, B5–A3, B5–A4, B5–A5 and B5–A6) for rhizogenesis, in which the content of macro- and microelements has been reduced by three times, the content of sucrose is reduced by almost 6 times, the amount of agar is reduced by almost five times, while the vitamin РР content is increased by 1.6 times, and the BAP is completely removed. The

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Kalashnikova, E. A., R. N. Kirakosyan, A. V. Gushchin, et al. "Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro." Vegetable crops of Russia, no. 6 (November 28, 2021): 22–29. http://dx.doi.org/10.18619/2072-9146-2021-6-22-29.

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Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new var

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Vasyukova, I., A. Gusev, O. Zakharova, P. Baranchikov, and N. Yevtushenko. "Silver nanoparticles for enhancing the efficiency of micropropagation of gray poplar (Populus × canescens Aiton. Sm.)." IOP Conference Series: Earth and Environmental Science 875, no. 1 (2021): 012053. http://dx.doi.org/10.1088/1755-1315/875/1/012053.

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Abstract The main purpose of the work was to determine the prospects for the use of silver nanoparticles in woody plants tissue culture and to identify possible limitations. The paper presents the results of experiments on the use of silver nanoparticles (AgNPs) to increase the efficiency of in vitro tissue culture of woody plant cultures using the example of gray poplar (Populus × canescens Aiton. Sm.) at various stages of microclonal reproduction. It has been shown that sequential treatment with 7.5 g/L NaOCl for 30 min and 0.3 g/L AgNPs for 5 min allows obtaining 100% phytopathogen-free exp

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Titenkov, A. V., M. N. Lushpin, T. N. Lushpina, N. V. Kotsareva, and A. N. Kryukov. "Adaptation of microclones of blackberries to in vivo conditions." IOP Conference Series: Earth and Environmental Science 845, no. 1 (2021): 012022. http://dx.doi.org/10.1088/1755-1315/845/1/012022.

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Abstract The results of studying the effect of mineral fertilizing on rhizogenesis and the development of aboveground organs of regenerant plants of blackberry thornless adaptable to in vivo conditions in the laboratory of selection, vegetable growing and horticulture, cloning “UNITS” Agrotechnopark “of Belgorod State Agrarian University are presented. Regenerated plants of thornless blackberry cultivar Agavam were adapted to in vivo conditions in a peat-perlite mixture with the addition of microelements and growth regulator root 16 days earlier than in the control. An active growth of the abo

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Fedorova, Yulia, Margarita Zaitseva, Larisa Fedorova, Mikhail Telpuk, and Alexey Pavlov. "Study of the Oxolin antiviral drug effect for the improvement of potato seed material during in vitro cultivation." E3S Web of Conferences 380 (2023): 01001. http://dx.doi.org/10.1051/e3sconf/202338001001.

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The article presents an analysis of the potato breeding problems in Russia which are associated with the low phytosanitary state of potato seed material infected with viral and bacterial diseases, as a result, it is necessary to carry out its improvement and the use of Russian varieties. Experiments were conducted in the laboratory of plant microclonal propagation according to the methodological recommendations for improvement and accelerated propagation of seed potatoes. The results of research on refining the technology of seed potato improvement by adding the Oxolin antiviral drug to the st

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Carey, Alisoun H., Uwe Claussen, Hermann-Josef L�decke, et al. "Interstitial deletions in DiGeorge syndrome detected with microclones from 22q11." Mammalian Genome 3, no. 2 (1992): 101–5. http://dx.doi.org/10.1007/bf00431253.

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Zakharova, O., E. Kolesnikova, D. Muratov, and A. Gusev. "Stimulating and toxic effects of graphene oxide on Betula pubescens microclones." IOP Conference Series: Earth and Environmental Science 595 (December 2, 2020): 012010. http://dx.doi.org/10.1088/1755-1315/595/1/012010.

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Tkachenko, Oksana V., Nina V. Evseeva, Kristina Y. Kargapolova, et al. "Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions." Microorganisms 11, no. 7 (2023): 1866. http://dx.doi.org/10.3390/microorganisms11071866.

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Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activitie

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Zakharova, Olga V., Alexander A. Gusev, Dmitry S. Muratov, Alexey V. Shuklinov, Nataliya S. Strekalova, and Sergey M. Matveev. "Titanium Trisulfide Nanoribbons Affect the Downy Birch and Poplar × Aspen Hybrid in Plant Tissue Culture via the Emission of Hydrogen Sulfide." Forests 12, no. 6 (2021): 713. http://dx.doi.org/10.3390/f12060713.

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Plant tissue culture is an important tool for accelerated vegetative reproduction of woody plants. To increase the efficiency of this method, it is necessary to search for new growth stimulators and protectors of microshoots. Two-dimensional (2D) nanomaterials are highly promising for applications in medicine and biotechnologies. We have studied the effects of TiS3 nanoribbons with the following mean dimensions: thickness less than 100 nm, length 1–10 μm and width 0.4–1 μm upon poplar × aspen hybrid and downy birch microclones in plant tissue culture. We have found the effectiveness of this pa

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Knyazeva, Inna, E. Kalashnikova, Danila Ilyushin, and Oksana Vershinina. "LED LIGHTING EFFECT ON THE ADAPTATION OF WATER MINT REGENERANT PLANTS IN AN ENVIRONMENTAL CHAMBER." Bulletin of KSAU, no. 10 (February 6, 2025): 41–47. https://doi.org/10.36718/1819-4036-2023-10-41-47.

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Growth rate, plant biomass and concentration of beneficial compounds largely depend on the quality and intensity of lighting. The purpose of research is to study LED lighting on the process of adaptation of regenerated watermint plants using a digital climate camera. During research, the object studied was microclones of watermint (Mentha aquatica L.). Watermint microclones were grown by in vitro cell and tissue culture in the laboratory of the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy. Subsequently, a set of measures was carried out to adapt plants in vitro to

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Evseeva, N. V., O. V. Tkachenko, A. Yu Denisova, G. L. Burygin, L. Yu Matora, and S. Yu Shchyogolev. "Effects of plant growth promoting Rhizobacteria on osmotically stressed potato microclones in vitro." Biomics 10, no. 2 (2018): 206–9. http://dx.doi.org/10.31301/2221-6197.bmcs.2018-29.

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Strekalova, Nataliya, Olga Zakharova, Petr Baranchikov, and Alexander Gusev. "INFLUENCE OF GRAPHENE OXIDE ON MICROCLONES OF DOWNY BIRCH AT THE ADAPTATION STAGE." Forestry Engineering Journal 11, no. 2 (2021): 48–58. http://dx.doi.org/10.34220/issn.2222-7962/2021.2/5.

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In this work, we studied the effect of graphene oxide on regenerants of downy birch when transferred to non-sterile greenhouse conditions (adaptation stage) during clonal micropropagation. During the experiment, it was found that a solution of graphene oxide at a concentration of 1.5 μg / l had a beneficial effect on the development of leaves and plant adaptability (+ 8%). However, with an increase in the concentration of nanomaterial to 3 μg / L, the survival rate decreased by 10% , the plant height - by 25%, and the number of adapted plants - by 35% relative to the control group. In addition

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Yu, Jingwei, Jianxin Qi, and Fa-Ten Kao. "Region-specific microdissection library and single-copy microclones for human chromosome 2p11–p13." Somatic Cell and Molecular Genetics 20, no. 2 (1994): 133–36. http://dx.doi.org/10.1007/bf02290682.

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Kalashnikova, E. A., L. A. GUD, A. A. ANISIMOV, R. N. KIRAKOSYAN, A. VASSILEV, and I. G. Tarakanov. "LIGHT SPECTRAL COMPOSITION EFFECTS ON RASBERRY AND BLACKBERRY MICROCLONES MORPHOPHYSIOLOGICAL TRAITS IN VITRO." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 2 (2020): 54–63. http://dx.doi.org/10.26897/0021-342x-2020-2-54-63.

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BARNAS, CHRISTOPH M., PATRICK ONYANGO, WILFRIED ELLMEIER, et al. "Determination and Regional Assignment of Grouped Sets of Microclones in Chromosome 1pter–p35." Genomics 29, no. 3 (1995): 607–15. http://dx.doi.org/10.1006/geno.1995.9006.

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Kucher, Nataliia, Volodymyr Hrabovyi, Olga Opalko, Volodymyr Zamorskyi, and Anatoly Opalko. "In vitro proparation of pear (Pyrus L.)." Studia Biologica 18, no. 4 (2024): 157–74. https://doi.org/10.30970/sbi.1804.797.

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Background. The micropropagation methods are used for the conservation of Pyrus L. phytodiversity, as well as for the creation of collections of the threatened species and pear cultivars including genotypes necessary for breeding and genetic studies. Materials and Methods. The two Pyrus species, P. communis, and P. salicifolia Pall. as well as four pear P. communis cultivars, ’Bere Desiatova’, ’Umans’ka Juvileina’, ’Kniahynia Olga’, and ’Sofia Umans’ka’, were chosen as the experimental plants. Young shoots of Pyrus species and cultivars with apical meristem from three-year-old plants were used

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Kalashnikova, Elena, Svetlana Zaytseva, Nguen Tan Thay, et al. "The role of poliphenols in formation of fungicidal activity and resistance to exometabolites of the fungus on the example of in vitro crops sunflower and savory." BIO Web of Conferences 40 (2021): 01014. http://dx.doi.org/10.1051/bioconf/20214001014.

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When studying the formation of secondary metabolites represented by phenolic compounds in callus cultures of sunflower plants, it was found that under in vitro conditions under the influence of a stress factor, the accumulation of polyphenols increases. It was found that the biosynthetic activity of callus cultures with respect to polyphenol increases in proportion to the concentration of the culture filtrate of the fungus (Sclerotinia Sclerotiorum) in the nutrient medium. This is confirmed by the qualitative and quantitative determination of the content of polyphenols in callus cultures. In s

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Lebed, N. I., A. M. Makarov, I. V. Volkov, M. P. Kukhtik, and M. B. Lebed. "Mathematical modeling of the process of sterilizing potato explants and obtaining viable potato microclones." IOP Conference Series: Earth and Environmental Science 786, no. 1 (2021): 012035. http://dx.doi.org/10.1088/1755-1315/786/1/012035.

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Arkhipova, Tatiana N., Nina V. Evseeva, Oksana V. Tkachenko, et al. "Rhizobacteria Inoculation Effects on Phytohormone Status of Potato Microclones Cultivated In Vitro under Osmotic Stress." Biomolecules 10, no. 9 (2020): 1231. http://dx.doi.org/10.3390/biom10091231.

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Water deficits inhibit plant growth and decrease crop productivity. Remedies are needed to counter this increasingly urgent problem in practical farming. One possible approach is to utilize rhizobacteria known to increase plant resistance to abiotic and other stresses. We therefore studied the effects of inoculating the culture medium of potato microplants grown in vitro with Azospirillum brasilense Sp245 or Ochrobactrum cytisi IPA7.2. Growth and hormone content of the plants were evaluated under stress-free conditions and under a water deficit imposed with polyethylene glycol (PEG 6000). Inoc

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Hampton, GarretM, Cathy Howe, Gabriele Leuteritz, et al. "Regional mapping of 22 microclones around the adenomatous polyposis coli (APC) locus on chromosome 5q." Human Genetics 88, no. 1 (1991): 112–14. http://dx.doi.org/10.1007/bf00204940.

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Zelenianska, N. M., and M. O. Samofalov. "Influence of the cultivation conditions of grape microclones in vitro on their survival in vivo." Taurian Scientific Herald, no. 127 (2022): 64–71. http://dx.doi.org/10.32851/2226-0099.2022.127.8.

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Weith, A., H. Winking, B. Brackmann, B. Boldyreff, and W. Traut. "Microclones from a mouse germ line HSR detect amplification and complex rearrangements of DNA sequences." EMBO Journal 6, no. 5 (1987): 1295–300. http://dx.doi.org/10.1002/j.1460-2075.1987.tb02367.x.

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Efimova, M. V., R. P. Litvinovskaya, Yu V. Medvedeva, et al. "Content and balance of endogenous brassinosteroids in potato microclones is determined by the organ specificity and term of ripening variety." Доклады Академии наук 485, no. 1 (2019): 117–21. http://dx.doi.org/10.31857/s0869-56524851117-121.

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The endogenous brassinosteroids content in the roots and shoots of early- and mid-season potato microclones was determined. Among the analyzed plant steroid hormones were representatives of brassinolide group, 24-epi- brassinolide group, 28-homobrassinolide group, B-lactones and B-ketons. The level of all brassinosteroids in plant roots exceeded significantly that in shoots, irrespective of the potato term of ripening. Of interest is the fact that in plant shoots there were no BS groups of 24-epibrassinolide. We have first found a relationship between the terms of potato ripening and endogenou

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Fujita, Shoichi, Eisei Shin, Tsutomu Nakamura, et al. "Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2." Japanese journal of human genetics 38, no. 4 (1993): 361–70. http://dx.doi.org/10.1007/bf01907982.

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Yalouskaya, N. A., J. N. Kalatskaja, N. A. Laman, K. S. Hileuskaya, V. I. Kulikouskaya, and V. V. Nikalaichuk. "Effect of the hydroxycinnamic acids and their chitosan-based conjugates on the growth and biochemical parameters of microclone potato plants <i>in vitro</i>." Doklady of the National Academy of Sciences of Belarus 66, no. 6 (2023): 605–13. http://dx.doi.org/10.29235/1561-8323-2022-66-6-605-613.

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This study determined the effect of exogenously applied hydroxycinnamic acids and their chitosan-based conjugates on growth and biochemical parameters of microclone potato plants (Solanum tuberosum L.) in vitro under optimal conditions. The findings of the present investigation confirm applying of chitosan-caffeic acid and chitosan-ferulic acid conjugates on microclone potato plants to improve growth and development. A significant growth-stimulating effect was found in caffeic acid and its chitosan-based conjugate. Chitosan behaved like a general elicitor inducing oxidative stress in plant cel

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Khoruzheva, T. I., S. A. Borovaya, and N. G. Boginskaya. "Establishing the <i>in vitro</i> culture of and micropropagating edible honeysuckle." Vegetable crops of Russia, no. 1 (February 14, 2024): 55–60. http://dx.doi.org/10.18619/2072-9146-2024-1-55-60.

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Edible honeysuckle is a popular fruit crop. Its therapeutic and health-promoting effects are attributed to a high content of bioactive compounds in the fruits. Unlike the traditional plant multiplication methods, the in vitro propagation allows scientists to obtain high-quality planting material of honeysuckle in a great quantity and within a short time. The research was carried out at the Laboratory of Breeding and Genetic Research on Field Crops of the Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaiki. Honeysuckle variety Podarok amurchanam creat

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Tkachenko, Oksana Viktorovna, Nina Vasilievna Evseeva, Kristina Yurievna Kargapolova, et al. "Increased activity of the pro/antioxidant system of potato microplants by rhizospheric bacterias in aeroponics conditions." Agrarian Scientific Journal, no. 3 (March 28, 2023): 65–72. http://dx.doi.org/10.28983/asj.y2023i3pp65-72.

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The improvement of plants in in vitro culture is an obligatory stage of potato seed production. Plant-growth-promoting rhizobacteria contribute to an increase in plant resistance to stress factors, including at the stage of adaptation of microplants to ex vitro conditions. The purpose of this investigation was to study the mechanisms of antioxidant protection of potato microclones during inoculation with rhizobacteria in the process of their adaptation to ex vitro conditions in an aeroponic installation. In experiments, microplants of potato Nevsky and Condor cultivars inoculated in vitro with

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Martinsson, Tommy, Andreas Weith, Celina Cziepluch, and Manfred Schwab. "Chromosome I deletions in human neuroblastomas: Generation and fine mapping of microclones from the distal Ip region." Genes, Chromosomes and Cancer 1, no. 1 (1989): 67–78. http://dx.doi.org/10.1002/gcc.2870010111.

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Grigoryan, M. A., A. A. Starchikov, O. V. Tkachenko, G. L. Burygin, and L. G. Kurasova. "Comparison of the effect of Azospirillum baldaniorum sp245 and exogenous indolyl-3-acetic acid on the growth of ten varieties of potato microclones in in vitro culture." IOP Conference Series: Earth and Environmental Science 1010, no. 1 (2022): 012162. http://dx.doi.org/10.1088/1755-1315/1010/1/012162.

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Abstract The article presents the results of a comparison of the effect of rhizospheric growth-stimulating bacteria of the Azospirillum baldaniorum Sp245 strain and exogenous indolyl-3-acetic acid on the growth of microclones of potato varieties Nevsky, Condor, Red Scarlett, Rosara, Avrora, Zhukovsky early, Ilyinsky, Vasilek, Fioletovy, Darenka at micropropagation in in vitro culture. The A. baldaniorum Sp245 strain is capable of synthesizing indolyl-3-acetic acid upon plant inoculation. Exogenous indolyl-3-acetic acid was introduced into the nutrient medium in three concentrations. The physio

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Soejima, H., K. Yoshiura, T. Tamura, et al. "Fifty novel sequence-tagged sites (STSs) on human chromosome 11q13.4→q25 identified from microclones generated by microdissection." Cytogenetic and Genome Research 70, no. 1-2 (1995): 108–11. http://dx.doi.org/10.1159/000134003.

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Yalouskaya, N. A., J. N. Kalatskaja, N. A. Laman, V. V. Nikalaichuk, A. N. Kraskouski, and K. S. Hileuskaya. "Caffeic Acid in Various Formulations as a Growth and Resistance Regulator of Potato Microclones in In Vitro Culture." Applied Biochemistry and Microbiology 59, no. 5 (2023): 686–93. http://dx.doi.org/10.1134/s0003683823050046.

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Теслюк, Н. І., and І. Аврамович. "IMPROVING OFADAPTATION METHODS OF PAVLOWNIYA TOMENTOSA MICROCLONES TO CONDITIONS IN VIVO WITH USE OF BACTERIA OF BACILLUS MEGATERIUM ONU50." Microbiology&Biotechnology, no. 3(47) (December 24, 2019): 92–102. http://dx.doi.org/10.18524/2307-4663.2019.3(47).182814.

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Tiong, Ing Soo, Satwica Yerneni, Tamia Nguyen, et al. "Venetoclax-Based Measurable Residual Disease Directed Therapy in Acute Myeloid Leukemia Is Associated with Emergent TP53 Microclones in Remission." Blood 144, Supplement 1 (2024): 51. https://doi.org/10.1182/blood-2024-201145.

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Venetoclax (VEN) combined with low-dose cytarabine (LDAC) is highly effective in acute myeloid leukemia (AML) with either measurable residual disease (MRD) or oligoblastic (5-15% blasts) relapse (VALDAC study) (Tiong et al, JCO 2024). We have previously shown that VEN-based therapy enhances selection of TP53 deficient leukemic clones with TP53 variants observed in 32% of patients (pts) at relapse (DiNardo et al, Blood 2020 and Thijssen et al, Blood 2021). In order to further understand the origins of these clones, we aimed to sensitively assess the clonal hematopoietic landscape in remission i

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