Journal articles: 'Micrococcus roseus'

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Wright, M. A., F. Taylor, S. J. Randles, D. E. Brown, and I. J. Higgins. "Biodegradation of a synthetic lubricant by Micrococcus roseus." Applied and Environmental Microbiology 59, no. 4 (1993): 1072–76. http://dx.doi.org/10.1128/aem.59.4.1072-1076.1993.

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Sarkar, A., and S. N. Upadhyay. "Purification and properties of cellulase from Micrococcus roseus." World Journal of Microbiology and Biotechnology 10, no. 6 (November 1994): 709–10. http://dx.doi.org/10.1007/bf00327966.

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Hereher, Faten, Amira ElFallal, Mohammed Abou-Dobara, Elshahat Toson, and Mohamed M. Abdelaziz. "Cultural optimization of a new exopolysaccharide producer “Micrococcus roseus”." Beni-Suef University Journal of Basic and Applied Sciences 7, no. 4 (December 2018): 632–39. http://dx.doi.org/10.1016/j.bjbas.2018.07.007.

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Kim, Bora, Ae-Jin Kim, and Jung-Kue Shin. "Effect of Sterilization by Intense Pulsed Light on Radiation-resistant Bacterium, Micrococcus roseus." Korean Journal of Food Science and Technology 45, no. 2 (April 30, 2013): 248–51. http://dx.doi.org/10.9721/kjfst.2013.45.2.248.

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Desai, Ashok E., and Pramila R. Bhamre. "Novel gut bacterial fauna of Gryllotalpa africana Beau. (Orthoptera : Gryllotalpidae)." International Journal of Life Sciences 6, no. 1 (May 28, 2012): 50–55. http://dx.doi.org/10.3126/ijls.v6i1.5949.

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Gryllotalpa africana (Beauvois) is a successful group of detritus feeder therefore it is an important both for their roles in carbon cycling in the environment and converting organic decaying material in to biofuel. Hence, bacterial diversity and relationship were explored from the mole cricket G.africana (Beau) to show ideal symbiotic association. The analysis and characterization of gut micro biota of G.africana (Beau) revealed the presence of 10 species and two genera of bacteria viz. Acidiphilium rubrum, Dienococcus proteolyticus, Sporosarcina ureae, Micrococcus varians, Micrococcus kristinae, Acetobactrium, Alcaligenes eutrophus, Micrococcus roseus, Micrococcus lylae, Sporosarcina, Citrobacter amalonaticus and Corynebacterium xerosis. All the species and genera were confirmed by observing their morphological features of the colony and various biochemical tests. These results indicate that the presence of mole cricket specific bacterial lineages implies relationship of gut microbes and their host mole cricket. The outcome of this work indicted that the gut bacterial fauna of G.africana shows the species-rich with greater diverse communities which might be providing resistance to invasion of pathogenic bacteria. DOI: http://dx.doi.org/10.3126/ijls.v6i1.5949

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SULE, Ismaila Olawale, and Israel Oluwatunmise ILORI. "Microbiological Assessment of Poultry Feeds within Ilorin, Nigeria." Notulae Scientia Biologicae 9, no. 1 (March 30, 2017): 34–39. http://dx.doi.org/10.15835/nsb9110025.

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The poultry feeds were obtained from 20 different poultry pens and their microbial contents were assessed. The antibiotics resistance patterns of the bacterial isolates were also determined. The bacterial count ranged from 5.0 × 103 to 1.76 × 106 cfu/g while the fungal count ranged from 3.5 × 104 to 1.9 × 105 cfu/g. The bacterial species isolated were Streptococcus salivarius, Streptococcus pyogenes, Micrococcus luteus, Micrococcus varians, Micrococcus roseus, Staphylococcus aureus, Staphylococcus saprophyticus and Staphylococcus hominis, while the fungal species isolated were Saccharomyces cerevisisae, Fusarium oxysporum, Penicillium sp., Humicola grisea, Aspergillus fumigatus, Hansenula sp. and Humicola fuscoatra. All the bacterial isolates were resistant to ceftazidime and cefuroxime and all the isolates were resistant to at least three antibiotics. Ofloxacin produced the highest zone of inhibition, followed by gentamicin, and then erythromycin. The presence of some pathogenic microorganisms in the poultry feeds revealed high level of contaminations. It is recommended that poultry feeds should be made from good quality grains and it should be prevented from environmental or other contamination.

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Chaibub, B. A., T. B. Oliveira, T. S. Fiuza, M. T. F. Bara, L. M. F. Tresvenzol, and J. R. Paula. "Composição química do óleo essencial e avaliação da atividade antimicrobiana do óleo essencial, extrato etanólico bruto e frações das folhas de Spiranthera odoratissima A. St.-Hil." Revista Brasileira de Plantas Medicinais 15, no. 2 (2013): 225–29. http://dx.doi.org/10.1590/s1516-05722013000200009.

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A Spiranthera odoratissima A. St.-Hil (manacá) é utilizada popularmente como depurativo do sangue, nas afecções renais e hepáticas (chá das folhas) para dores musculares, de estômago, de cabeça, e disfunções hepáticas (chá das raízes). O objetivo desse trabalho foi avaliar a composição química do óleo essencial e a atividade antimicrobiana do óleo essencial, do extrato etanólico bruto e frações obtidos das folhas de S. odoratissima contra bactérias Gram positivas e negativas, e Candida albicans. O extrato bruto das folhas foi obtido por maceração seguido de concentração em rotaevaporador e as frações por partição em coluna filtrante. O pó das folhas foi submetido à hidrodestilação em aparelho de Clevenger e o óleo essencial obtido foi analisado por CG/EM. A atividade antimicrobiana foi avaliada pelo método da diluição em ágar para determinar a concentração inibitória mínima (CIM). Os constituintes majoritários do óleo essencial foram β-cariofileno (20,64%), γ-muuroleno (17,70%), biciclogermacreno (14,73%), e δ-cadineno (13,40%). No estudo da atividade antimicrobiana de S. odoratissima, os principais resultados foram obtidos contra Staphylococus epidermidis (extrato etanólico bruto, CIM de 0,098 mg/mL), C. albicans (fração hexano, CIM de 0,049 mg/mL), Bacillus cereus (diclorometano, CIM de 0,098 mg/mL), Micrococcus roseus (fração acetato de etila, CIM 0,049 mg/mL), e M. roseus, Micrococus luteus, B. cereus e C. albicans (fração metanol, CIM de 0,391 mg/mL).

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Paul, Jaishree, and A. K. Varma. "Hydrolytic enzyme(s) production in Micrococcus roseus growing on different cellulosic substrates." Letters in Applied Microbiology 16, no. 3 (March 1993): 167–69. http://dx.doi.org/10.1111/j.1472-765x.1993.tb01386.x.

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Yolmeh, Mahmoud, Morteza Khomeiri, Mohammad Ghorbani, Ezzatollah Ghaemi, and Seyyedeh Sanaz Ramezanpour. "High efficiency pigment production from Micrococcus roseus (PTCC 1411) under ultraviolet irradiation." Biocatalysis and Agricultural Biotechnology 9 (January 2017): 156–61. http://dx.doi.org/10.1016/j.bcab.2016.12.010.

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Rostami, Hossein, Hassan Hamedi, and Mahmoud Yolmeh. "Some biological activities of pigments extracted from Micrococcus roseus (PTCC 1411) and Rhodotorula glutinis (PTCC 5257)." International Journal of Immunopathology and Pharmacology 29, no. 4 (October 10, 2016): 684–95. http://dx.doi.org/10.1177/0394632016673846.

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The importance of replacing synthetic pigments with natural types is increasing day by day in the food industry due to the harmful effects of some synthetic pigments. Microorganisms are a major source of natural pigments, which nowadays have attracted the attention of researchers. In this study, carotenoid pigments were produced by Micrococcus roseus and Rhodotorula glutinis, and some of their biological properties such as antimicrobial, antioxidant, anticancer, and anti-inflammatory activities were evaluated. Given the results, bacteria, especially gram-positive bacteria, had higher sensitivity to the pigments extracted from M. roseus (PEM) and R. glutinis (PER) compared to molds so that Bacillus cereus and Alternaria citri had the highest and the lowest sensitivity, respectively. PER showed a higher antioxidant activity compared with PEM in the various methods of measuring antioxidant activity. In vitro and in vivo anti-tumor-promoting activities of PER were measured significantly more than PEM ( P <0.05). Both pigment extracts remarkably inhibited the 12- O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation, so that ID50 (50% inhibitory dose) of PEM and PER were 0.22 and 0.09 mg/ear, respectively.

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Naznin, Rezuana, Nasrin Sultana, Md Nur Hossain, Mohammad Nurul Islam, Anika Tabassum, Md Ataul Gani, and Mahbubah Jannat. "Conventional and Molecular Identification of Culturable Airborne Bacteria." Plant Tissue Culture and Biotechnology 30, no. 1 (June 25, 2020): 15–25. http://dx.doi.org/10.3329/ptcb.v30i1.47787.

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Bacteria isolated from the environment during the present study were representative of normal microflora of the skin, respiratory and urinary tracts; it also includes some soil and water-borne pathogenic and nonpathogenic genera. Six samples from different locations were studied for bacterial investigation. Among 14 isolates obtained, 13 were Gram positive, and the rest one was Gram negative. Out of 13 Gram positive isolates, 12 were round-shaped non spore forming and were identified as Planococcus citreus, Stomatococcus mucilaginosus, Kocuria kristinae, Micrococcus agilis (2), Kytococcus sedentarius (2), Micrococcus luteus, Micrococcus lylae and M. roseus, Staphylococcus aureus, Staphylococcus epidermidis and rod-shaped non spore forming identified as Renibacterium salmoninarum. The Gram-negative bacteria was identified as Pseudomonas aeruginosa. Other than provisional identification, two isolates (JG 40 and SG 49) were further confirmed through molecular characterization on the basis of 16Sr RNA gene sequence analysis as Staphylococcus aureus and Pseudomonas aeruginosa repectively. Spearman’s correlation showed that air temperature and wind speed negatively correlated with the bacterial abundance. It is clear that none of the samples containing airborne pathogens collected was safe for human health due to presence of potentially pathogenic microorganisms. Many were human pathogenic as well as food poisoning microorganisms. Plant Tissue Cult. & Biotech. 30(1): 15-25, 2020 (June)

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Stamopoulos, C., and Ν. Μ. Tzanetakis. "Βacterial flora isolated from the oesophageal bulb of the olive fruit fly Dacus oleae (Gmelin)." ENTOMOLOGIA HELLENICA 6 (May 31, 2017): 43. http://dx.doi.org/10.12681/eh.13957.

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In a study of the bacterial flora occurring in the adults of the olive fruit fly, Dacus oleae (Gmelin) (Diptera: Tephritidae), oesophageal diverticulum, a total of 28 strains were obtained. Six of them were Gram – negative and identified as Pseudomonas niendocina, Morarella nonliquefasciens (2), Alcaligenes sp., Enterobacter cloacae (2) and 22 Gram-positive classified as Kurthia sp., Staphylococcus subgroup VI, Micrococcus roseus, Bacillus pumilus, B. licheniformis (3) and B. subtilis(15). None of the above bacteria are strictly fixed and constantly present in the oesophageal diverticulum, suggesting that the bacterial flora associated with D. oleae depends on environmental factors, and could be used as a nutrient source for the insect apart from its possible other symbiotic role.

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TORNADIJO, MARÍA E., JOSÉ M. FRESNO, JAVIER CARBALLO, and ROBERTO MARTÍN SARMIENTO. "Population Levels, Species, and Characteristics of Micrococcaceae during the Manufacturing and Ripening of Armada-Sobado Hard Goat's Milk Cheese." Journal of Food Protection 59, no. 11 (November 1, 1996): 1200–1207. http://dx.doi.org/10.4315/0362-028x-59.11.1200.

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The changes in the counts and the species of Micrococcaceae were studied throughout the manufacturing and ripening of a Spanish hard goat's milk cheese, the Armada-Sobado variety. In the milk, counts on mannitol salt agar (MSA) ranged from 2 × 104 to 5 × 104 CFU/g. These counts showed the maximum value in the curd (7 × 104 to 4 × 105 CFU/g), decreasing afterwards slowly but steadily throughout the ripening process to reach final counts on average 2 logarithmic units lower than those found in the curd. Of 280 isolates obtained from MSA during manufacturing and ripening, 66 (24%) were considered to be Micrococcaceae. Staphylococcus sciuri (22.5% of the isolates at this sampling point) and Staphylococcus saprophyticus (7.5%) were the only two species of staphylococci isolated from the milk. In the curd, S. sciuri increased its proportion (30%) whilst the percentage of S. saprophyticus remained constant. None of these species was isolated from the cheese. S. aureus was detected only in curd (7.5% of the isolates obtained at this sampling point). S. xylosus, S. capitis, S. epidermidis, and S. warneri were isolated from curd and cheese, or exclusively from cheese, but always in very low proportions. Micrococcus varians (10%) and M. roseus (5%) were the two species of micrococci isolated from the milk. M. varians increased its proportion in curd (17.5%) and could not be isolated in cheese. M. roseus appeared neither in curd nor in cheese. All the isolated staphylococcal strains were tested for production of A, B, C, and D enterotoxins. The three isolated strains of Staphylococcus aureus produced A and C enterotoxins, but neither B or D. Of 41 coagulase-negative strains only two of the Staphylococcus sciuri isolated from milk produced C enterotoxins.

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Bustamante, K. G. L., A. D. F. Lima, M. L. Soares, T. S. Fiuza, L. M. F. Tresvenzol, M. T. F. Bara, F. C. Pimenta, and J. R. Paula. "Avaliação da atividade antimicrobiana do extrato etanólico bruto da casca da sucupira branca (Pterodon emarginatus Vogel) - Fabaceae." Revista Brasileira de Plantas Medicinais 12, no. 3 (September 2010): 341–45. http://dx.doi.org/10.1590/s1516-05722010000300012.

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Pterodon emarginatus Vogel (Fabaceae) é uma árvore do Cerrado conhecida popularmente como "sucupira branca, faveiro, fava de sucupira e sucupira lisa" e utilizada na medicina popular em preparações anti-reumáticas, antiinflamatórias, analgésicas e antiinfecciosas. Esse trabalho teve por objetivo fazer a triagem fitoquímica do pó e avaliar a atividade antimicrobiana do extrato etanólico bruto das cascas da P. emarginatus contra bactérias Gram-positivas, Gram-negativas e o fungo Candida albicans. O extrato etanólico bruto foi obtido a partir das cascas dessecadas e pulverizadas. A concentração inibitória mínima (CIM) do extrato bruto foi determinada utilizando-se o inoculador de Steers. Os testes fitoquímicos detectaram a presença de flavonóides, heterosídeos saponínicos, resinas e traços de esteróides e triterpenóides. As CIM do extrato etanólico foram de 0,18 mg mL-1 para as bactérias Gram-positivas Rhodococcus equi ATCC 25923, Micrococcus luteus ATCC 9341, Micrococcus roseus IPTSP/UFG e para as bactérias Gram-negativas Serratia marcescens ATCC 14756 e Pseudomonas aeruginosa ATCC 9027; de 0,37 mg mL-1 para a Enterobacter cloacae FT 505 LEMC/EPM/UFG e de 0,74 mg mL-1 para as demais bactérias testadas e para o fungo C. albicans. O presente estudo abre perspectivas para o uso da cascas da P. emarginatus como antimicrobiano.

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Jagannadham, M. V., V. J. Rao, and S. Shivaji. "The major carotenoid pigment of a psychrotrophic Micrococcus roseus strain: purification, structure, and interaction with synthetic membranes." Journal of Bacteriology 173, no. 24 (1991): 7911–17. http://dx.doi.org/10.1128/jb.173.24.7911-7917.1991.

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Strand, Aase, Sisinthy Shivaji, and Synnøve Liaaen-Jensen. "Bacterial carotenoids 55. C50-carotenoids 25.† revised structures of carotenoids associated with membranes in psychrotrophic Micrococcus roseus." Biochemical Systematics and Ecology 25, no. 6 (September 1997): 547–52. http://dx.doi.org/10.1016/s0305-1978(97)00039-2.

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Giani, Micaela, Inés Garbayo, Carlos Vílchez, and Rosa María Martínez-Espinosa. "Haloarchaeal Carotenoids: Healthy Novel Compounds from Extreme Environments." Marine Drugs 17, no. 9 (September 6, 2019): 524. http://dx.doi.org/10.3390/md17090524.

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Haloarchaea are halophilic microorganisms belonging to the archaea domain that inhabit salty environments (mainly soils and water) all over the world. Most of the genera included in this group can produce carotenoids at significant concentrations (even wild-type strains). The major carotenoid produced by the cells is bacterioruberin (and its derivatives), which is only produced by this kind of microbes and few bacteria, like Micrococcus roseus. Nevertheless, the understanding of carotenoid metabolism in haloarchaea, its regulation, and the roles of carotenoid derivatives in this group of extreme microorganisms remains mostly unrevealed. Besides, potential biotechnological uses of haloarchaeal pigments are poorly explored. This work summarises what it has been described so far about carotenoids from haloarchaea and their production at mid- and large-scale, paying special attention to the most recent findings on the potential uses of haloarchaeal pigments in biomedicine.

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Hanon, Noor Ameer, and Fouad Najm Abd. "The Antimicrobial Activity of Quercus Infectoria Extracts Against Bacteria Isolated from Wounds Infection." Al-Mustansiriyah Journal of Science 32, no. 1 (February 21, 2021): 1. http://dx.doi.org/10.23851/mjs.v32i1.919.

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Bacteria that cause wound infections were isolated and diagnosed from patients, who were hospitalized at the Martyr Al-Sadr Hospital from October 2019 to April 2020. Antibacterial effect of Quercus infectoria extract (aqueous and alcoholic) on the isolated bacteria has been studied. The results showed that the alcoholic extract has a higher antibacterial effect than the aqueous extract against Gram-positive bacteria like Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus except Micrococcus roseus, which the effect of aqueous extract was higher than alcoholic extract, whereas aqueous and alcoholic extracts showed the same effect against Streptococcus sp.. The alcoholic extract has higher antibacterial effect than the aqueous extract against Gram-negative bacteria (Escherichia coli, Pseudomonas sp., Enterococcus faecalis, Shigella dysnteriae, Enterobacter sakazkii, citrobcter freundii and Alcaligenes faecalis) except proteus sp., which the aqueous extract was higher than alcoholic extract.

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Yadav, Amresh Kumar, P. Kumar, A. Kumari, and MP Trivedi. "Identification and Prevention of Microbial Contamination in Tissue Culture of Catharanthus roseus - An Important Medicinal Herb." Plant Tissue Culture and Biotechnology 30, no. 2 (December 11, 2020): 297–305. http://dx.doi.org/10.3329/ptcb.v30i2.50699.

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The explants of two varities rosea and alba of Catharanthus roseus used for in vitro propagation and found to be more than 50% of the cultures became contaminated. The most common bacterial contaminants were Bacillus licheniformis, Micrococcus, Panibacillus and fungal contaminants were Fusarium, Alternaria, Cladosporium and Aspergillus. Combinations of different antibiotics (Penicillin, norfloxacin, tobramycin, gatifloxacin, ofloxacin) and fungicides (Bavastin, captan, fluconazole and trichoderma) were used to control the growth of the contaminants. Gatifloxacin and ofloxacin inhibited 100% growth of bacteria whereas, bavastin and captan appeared to be the most effective fungicides. Combination of gatifloxacin, ofloxacin with bavastin and captan inhibited the growth of contaminants at their minimum phytotoxic concentration (MIC). The observed minimum phytotoxic concentration (MPC) of ofloxacin, gatifloxacin, bavastin and captan was 15, 9, 6 and 5% at their respective MIC. More than 90% of the cultures responded for callus formation in the combination of gatifloxacin (4%) + bavastin (1%). While the combination of gatifloxacin and captan was highly toxic that reduces the growth of the culture. Plant Tissue Cult. & Biotech. 30(2): 297-305, 2020 (December)

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Jagannadham, M. V., M. K. Chattopadhyay, and S. Shivaji. "The Major Carotenoid Pigment of a Psychrotrophic Micrococcus roseus Strain: Fluorescence Properties of the Pigment and Its Binding to Membranes." Biochemical and Biophysical Research Communications 220, no. 3 (March 1996): 724–28. http://dx.doi.org/10.1006/bbrc.1996.0471.

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Hinrichsen, L. L., M. C. Montel, and R. Talon. "Proteolytic and lipolytic activities of Micrococcus roseus (65), Halomonas elongata (16) and Vibrio sp. (168) isolated from Danish bacon curing brines." International Journal of Food Microbiology 22, no. 2-3 (May 1994): 115–26. http://dx.doi.org/10.1016/0168-1605(94)90136-8.

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Joseph, Babu, Supriya Upadhyaya, and Pramod Ramteke. "Production of Cold-Active Bacterial Lipases through Semisolid State Fermentation Using Oil Cakes." Enzyme Research 2011 (April 26, 2011): 1–6. http://dx.doi.org/10.4061/2011/796407.

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Production of cold active lipase by semisolid state fermentation involves the use of agroindustrial residues. In the present study, semisolid state fermentation was carried out for the production of cold active lipase using Micrococcus roseus, isolated from soil samples of Gangotri glaciers, Western Himalayas. Among various substrate tested, groundnut oil cake (GOC) favored maximal yield of lipases at 15 ± 1°C within 48 h. Supplementation of glucose 1% (w/v) as additional carbon source and ammonium nitrate 2% (w/v) as additional nitrogen source enhanced production of lipase. Addition of triglycerides 0.5% (v/v) tends to repress the lipase production. Further mixed preparation of groundnut oil cake (GOC) along with mustard oil cake (MOC) in the ratio of 1 : 1, and its optimization resulted in improved production of cold active lipase. The enzyme exhibited maximum activity at 10–15°C and was stable at temperatures lower than 30°C. The lipase exhibited optimum activity at pH 8 and showed more than 60% stability at pH 9. Semisolid state fermentation process by utilizing agroindustrial wastes will direct to large-scale commercialization of lipase catalyzed process in cost-effective systems.

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Braide, W., Ajugwo, G.C, Adeleye, S.A., Mike-Anosike E.E., Ndukwe, C.U, and Chinakwe E.C. "CORRELATION ANALYSIS BETWEEN INDOOR AIRBORNE BACTERIAL LOAD AND OCCUPANT DENSITY IN HOSPITAL INDOOR AIR." International Journal of Engineering Technologies and Management Research 6, no. 5 (March 25, 2020): 73–83. http://dx.doi.org/10.29121/ijetmr.v6.i5.2019.373.

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A study of the quantity and types of airborne bacteria and its correlation with human presence was conducted. Air samples were collected from different units for three days and three sessions (morning, afternoon and evening) for the enumeration and identification of bacterial isolates. Walk-through exercise was also conducted prior to every sampling to gather information on the number of occupants present, activities going on, and room characteristics. Isolation study revealed higher bacterial load in the afternoon and evening sessions; with Male Ward and Operating Theatre recording the highest and lowest bacterial loads respectively, as compared to the morning session that was done immediately after cleaning and before influx of people. The Spearman’s Correlation Coefficient showed a positively direct linear correlation between the bacterial load and occupant population irrespective of the three sessions (r = 0.84, 0.88 and 0.93). Identification study showed that the isolates are representatives of normal microflora of the skin, respiratory and gastro-intestinal tracts which includes the following; Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus roseus, Klebsiella pneumoniae, Proteus mirabilis, Bacillus subtilis, Aspergillus, Penicillium, Mucor, Candida and Fusarium species. The study presents evidence of increased concentration of indoor airborne bacteria due to human presence, movement and activities.

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RÎMBU, Cristina, Cristina HORHOGEA, Andreea COZMA, Carmen CRETU, Mariana GRECU, Raluca RUSU, and Eleonora GUGUIANU. "Analysis of Bacteriological Infected Dog and Cat Bite Wounds in Veterinary Medical Staff." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 77, no. 1 (June 3, 2020): 43. http://dx.doi.org/10.15835/buasvmcn-vm:2019.0038.

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Bite wounds, human and animal origin, can lead to significant complications if appropriate therapy is not undertaken timeously. The major risk of these aggressions is given by the possibility of systemic propagation of the bacteria involved in the wound and the appearance of complications such as osteomyelitis, septic arthritis, bacterial endocarditis, etc. The identification of pathogenic germs and antibiotic sensitivity test were the key points for a specific therapy that could prevent the spread of infection in the deep tissues. Twenty five biological samples collected from various wounds produced by dogs and cats bites over the veterinary personnel during the medical evaluated. Various aerobic pathogenic and conditional pathogenic bacteria (Staphylococcus aureus, Staphylococcus pseudo intermedius, Staphylococcus intermedius, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus xylosus, Staphylococcus warneri, Micrococcus roseus, Streptococcus sp. gr.B, Streptococcus sp. gr.F, Streptococcus sp. gr.G, Enterococcus faecalis, Enterococcus faecium, Trueperella pyogenes, Corynebacterium sp., Bacillus cereus, Pasteurella multocida, Pasteurella canis, Mannheimia haemolitica, Escherichia coli, Pseudomonas aeruginosa, Actinobacillus sp., Neisseria sp) and anaerobic bacteria (Clostridium perfringens, Bacteroides sp.) were isolated. The bacterial drug sensitivity was fluctuating, observing the variations influenced by the natural and acquired resistance of the tested bacterial species. The wounds produced by the bites of dogs and cats have aerobic and anaerobic polymicrobial etiology.

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Karim, Md Abdul, and Nasrin Sultana. "Diversity and Antibiotic Susceptibility of Bacteria in Water of Hotel Restaurants in Dhaka City." Journal of the Asiatic Society of Bangladesh, Science 43, no. 2 (December 22, 2017): 173–80. http://dx.doi.org/10.3329/jasbs.v43i2.46515.

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Present study was conducted to determine the microbiological status of water from dispensers in different roadside hotel and restaurants of Dhaka city. Samples were collected from seven hotel and restaurants. Aerobic heterotrophic bacterial count ranged between 1.5 × 10 and 8.8 × 103 cfu/ml. Enteric and related bacterial abundance in MacConkey, SS and Cetrimide agar media ranged from 0 to 4.9 × 106, 0 to 2.1 × 105 and 0 to 1.2 × 106cfu/ml, respectively. In total, 28 bacterial isolates were obtained during the study period. Among them, 15 were heterotrophic isolates and 13 were enteric and related bacteria. Among 15 aerobic heterotrophic isolates, 11 were gram positive and five were gram negative. Out of 11 gram positive isolates, 7 belonged to the genus Bacillus viz. B. circulans, B. subtilis, B. stearothermophilus, B. brevis and B. cereus and one to coccus viz. Micrococcus roseus. The other gram positive species were Kurtia gibsonii, Auriobacterium liguefaciens and Curtobacterium luteum. Four gram negative isolates were Neisseria elongate sub. spp. glycolytica, Plesiomonas shigelloides, Pseudomonas fluorescens biovar 1, Pseudomonas aeruginosa. All 13 enteric and related isolates were gram negative, short rod; and non-spore formers and belonged to the genera Escherichia, Klebsiella, Shigella and Pseudomonas. Among all isolates, two were resistant and six were susceptible to all five antibiotics. Asiat. Soc. Bangladesh, Sci. 43(2): 173-180, December 2017

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Afoshin, Alexey S., Mihail A. Konstantinov, Ilya Yu Toropygin, Irina V. Kudryakova, and Natalia V. Vasilyeva. "β-Lytic Protease of Lysobacter capsici VKM B-2533T." Antibiotics 9, no. 11 (October 28, 2020): 744. http://dx.doi.org/10.3390/antibiotics9110744.

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Bacteriolytic enzymes are promising antimicrobial agents for developing new-generation drugs. Recently, we have isolated a β-lytic protease (BlpLc) from the culture liquid of Lysobacter capsici VKM B-2533T. This BlpLc possesses a valuable property, not described for β-lytic proteases (Blps) earlier, of hydrolyzing living cells of Staphylococcus aureus 55 MRSA clinical isolate. This work phylogenetically characterized the BlpLc and investigated its properties. Analysis revealed a variability of pre-/pro-parts of Blp precursors. The mature BlpLc is the closest to the earlier annotated but not isolated Blp from Lysobacter sp. Root690. The biochemical characterization found conditions for the BlpLc general bacteriolytic activity relative to autoclaved S. aureus 209P cells to differ from that of earlier isolated Blp. Unexpected was the effect of serine (phenylmethylsulfonyl fluoride (PMSF)) and cysteine (p-chloromercuribenzoate (p-CMB)) protease inhibitors on BlpLc bacteriolytic and proteolytic activities. The specificity of BlpLc proteolytic action relative to hemoglobin, elastin, gelatin, collagen, azofibrin, myoglobin, ovalbumin, and ovamucoid was found. New types of peptide bonds—Gly-X, Ser-X, Lys-X, Ala-X, Val-X, Glu-X, and Phe-X—hydrolyzed by the enzyme in protein substrates were first revealed using MALDI-TOF. Turbidimetrically, the BlpLc was found to lyze living cells of S. aureus 209P, Micrococcus luteus B1819, and M. roseus B1236, which is important for expanding the enzyme’s applied properties.

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Kaziev, Alibek A., Amantay Sh Akzhigitov, Sofia Z. Sagyndykova, and Azamat N. Nurlybekov. "Field testing of immobilized oil-degrading microorganisms in the Zhanatalap field of Atyrau region." Butlerov Communications 62, no. 5 (May 31, 2020): 142–50. http://dx.doi.org/10.37952/roi-jbc-01/20-62-5-142.

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Hydrocarbon-oxidizing microorganisms are widespread in natural ecosystems, since the ability to oxidize hydrocarbons is associated with the presence of enzymes of the oxidase group, with microorganisms using oil and oil products as the sole source of carbon and energy in the microbiological decomposition of hydrocarbons. The decomposition of oil and oil products in the soil under natural conditions is a biogeochemical process in which the functional activity of a complex of soil microorganisms that ensure the complete mineralization of oil and oil products to carbon dioxide and water is of crucial importance. The search for effective indigenous hydrocarbon-oxidizing microorganisms, the creation on their basis of a full-fledged specialized consortium of microorganisms and their introduction into the initial, cleaned environment is one of the promising methods of purification with oil pollution in the oil-producing regions of Kazakhstan. Among the new biological methods for cleaning soil from oil pollution, the most promising are the use of a consortium based on immobilized microorganisms. A correctly selected carrier capable of sorption of petroleum hydrocarbons preserves and maintains attached cells in a viable state for a long time and protects them, especially at the initial stage of introduction, from adverse environmental conditions, which increases the efficiency of oil destruction. Hydrocarbon-oxidizing microorganisms have high emulsifying activity and sorption ability for expanded clay, optimal conditions for biomass accumulation are: temperature 28-30 °С, pH = 7 and 9. It is recommended to use a consortium of oil-degrading microorganisms based on the native strains of cultures Bacillus firmus S20, Bacillus subtilis PR28, Micrococcus roseus UD6-4, Micrococcus varians PR69 to clean the soils contaminated with oil and oil products in the Zhanatalap field in the Atyrau region. It should be noted that after 8 months at the Zhanatalap field in the Atyrau region, oil destruction amounted to 94.3% in the variant with the introduction of immobilized microorganisms on zeolite and expanded clay. The results of the research are the basis for further development of technology for the restoration of oil-contaminated soils in arid conditions of Kazakhstan.

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Mahato, Shashi B., and Subhadra Garai. "ChemInform Abstract: Microbial Biotransformation of Cholic Acid to the New Potential Steroid Intermediate, 9α-Hydroxy-3,12-dioxo-23,24-dinorchola-4,6-dienoic Acid by Micrococcus roseus." ChemInform 31, no. 44 (October 31, 2000): no. http://dx.doi.org/10.1002/chin.200044202.

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Mahato, Shashi B., and Subhadra Garai. "Microbial biotransformation of cholic acid to the new potential steroid intermediate, 9 α-hydroxy-3, 12-dioxo-23, 24-dinorchola-4,6-dienoic acid by Micrococcus roseus." Journal of Chemical Research 2000, no. 6 (June 1, 2000): 278–79. http://dx.doi.org/10.3184/030823400103167282.

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Darma, Umar Zubairu, Aisha Zango Mansir, and Yunusa Yahaya Riko. "Compatibility and formulation of diesel degrading consortia using bacteria isolated from contaminated soil." Bayero Journal of Pure and Applied Sciences 12, no. 1 (April 15, 2020): 199–208. http://dx.doi.org/10.4314/bajopas.v12i1.32s.

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Soil contamination with diesel spillage is an increasing environmental challenge that damages living ecosystems. Efficiency of single bacterium in degrading diesel oil pollutants is faced with slow performance limitation. Therefore, the use of consortia is shown to be better, due to synergism, multi-enzymatic activity and potential for diversified catabolic functionalities. This study is aimed at formulating effective bacterial consortia that can degrade diesel in polluted environments. Four diesel degrading bacteria as Bacillus subtilis, Staphylococcus aureus, Micrococcus roseus and Rhodococcus specie were isolated and used for consortia formulation. Purity testing was performed on the isolates prior to consortia formulation, before their compatibility was tested by cross-spreading them on nutrient agar. Consortia formulation was made using Bacteria resting cells in Phosphate Buffer Saline based on compatibility testing and mathematical permutations. For on their ability to survive diesel on Bushnell-Haas Agar (BHA), consortia 2, 9 and 11 showed the best results among which consortium 11 was chosen as the best, considering growths on the medium within a 72 hrs period. The growth of the organisms before consortia formulation and after was also evaluated, which suggest that the consortium perform better than individual strains. Analysis of Variance showed significant statistical differences (p<0.05) between constituents of consortia, and diesel degradation on 2% (v/v) BHA. The degradation performances of the various consortia on BHA were furthermore separated by Duncan’s Multiple Range Test. The colony counts obtained indicate that degradation was performed better by the consortia than individual strains. The findings of the study contribute towards illuminating inter-microbial relationships and microbial ecology especially within groups of diesel degrading bacteria. Further studies are imperative, to maximally harness the potentials of these bacteria for applications in large scale diesel biodegradation. Key Words: Consortia, Diesel, Bacterial Biodegradation, Haemocytometry.

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Elamary, Rokaia, and Wesam M. Salem. "Optimizing and purifying extracellular amylase from soil bacteria to inhibit clinical biofilm-forming bacteria." PeerJ 8 (November 2, 2020): e10288. http://dx.doi.org/10.7717/peerj.10288.

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Background Bacterial biofilms have become a major threat to human health. The objective of this study was to isolate amylase-producing bacteria from soil to determine the overall inhibition of certain pathogenic bacterial biofilms. Methods We used serial dilution and the streaking method to obtain a total of 75 positive amylase isolates. The starch-agar plate method was used to screen the amylolytic activities of these isolates, and we used morphological and biochemical methods to characterize the isolates. Optimal conditions for amylase production and purification using Sephadex G-200 and SDS-PAGE were monitored. We screened these isolates’ antagonistic activities and the purified amylase against pathogenic and multi-drug-resistant human bacteria using the agar disk diffusion method. Some standard antibiotics were controlled according to their degree of sensitivity. Finally, we used spectrophotometric methods to screen the antibiofilm 24 and 48 h after application of filtering and purifying enzymes in order to determine its efficacy at human pathogenic bacteria. Results The isolated Bacillus species were Bacillus megaterium (26.7%), Bacillus subtilis (16%), Bacillus cereus (13.3%), Bacillus thuringiesis (10.7%), Bacillus lentus (10.7%), Bacillus mycoides (5.3%), Bacillus alvei (5.3%), Bacillus polymyxa (4%), Bacillus circulans (4%), and Micrococcus roseus (4%). Interestingly, all isolates showed a high antagonism to target pathogens. B. alevi had the highest recorded activity (48 mm) and B. polymyxa had the lowest recorded activity (12 mm) against Staphylococcus aureus (MRSA) and Escherichia coli, respectively. On the other hand, we detected no antibacterial activity for purified amylase. The supernatant of the isolated amylase-producing bacteria and its purified amylase showed significant inhibition for biofilm: 93.7% and 78.8%, respectively. This suggests that supernatant and purified amylase may be effective for clinical and environmental biofilm control. Discussion Our results showed that soil bacterial isolates such as Bacillus sp. supernatant and its purified amylase are good antibiofilm tools that can inhibit multidrug-resistant former strains. They could be beneficial for pharmaceutical use. While purified amylase was effective as an antibiofilm, the isolated supernatant showed better results.

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Aderonke, Akintokun K., Onatunde O. Oladimeji, Olufunke B. Shittu, Itunu C. Okeyode, and Michael O. Taiwo. "Bioaccumulation of Heavy Metals using Selected Organisms Isolated from Electronic Waste Dumpsite of two South-Western States in Nigeria." Applied Environmental Research, June 30, 2017, 29–40. http://dx.doi.org/10.35762/aer.2017.39.2.3.

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Heavy metals from electronic wastes can accumulate to alarming concentrations in soils, causing significant detrimental impacts on human life and the environment. Bioaccumulation of heavy metals by bacteria and fungi has been a major focus of most bioremediation studies owing to the excellent metal-binding properties. The current study was conducted to isolate the most promising Zn, Cu and Pb tolerant microorganisms from contaminated soils, and to assess their metal accumulating abilities. Bacillus licheniformis, B. polymyxa, Pseudomonas aeruginosa, Micrococcus roseus, Aspergillus niger and A. flavus were selected for the bioaccumulation study, based on their known tolerance to heavy metals. Bacillus licheniformis was most efficient in the removal of Cu (71.3 %) and Pb (70.1 %). Pb accumulation for Aspergillus flavus was 65.76 %. Zn accumulation for Pseudomonas aeruginosa and Aspergillus niger were 74.1 % and 78.3 %, respectively. This study concluded that all these microorganisms have potential for bioremediating soil environments contaminated with heavy metals.

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Ko, W. H., B. H. Chen, and C. W. P. Chiu. "Effect of incubation conditions on the reduction of nitrate to nitrite by Micrococcus roseus and Escherichia coli O157: H7." Journal of Food and Drug Analysis 11, no. 2 (July 14, 2020). http://dx.doi.org/10.38212/2224-6614.2721.

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Biswas, Suparna Mandal, Nasrin Begam, Sunanda Chanda, and Lalit Kumar. "Allelochemicals from the leaves of Tamarindus indica L." Annals of Tropical Research, April 13, 2009. http://dx.doi.org/10.32945/atr3113.2009.

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Potent biopesticides with strong antimicrobial activity as well growth inhibitory activity has been isolated and purified from the Ethyl Acetate Fraction (TrEAF) of tamarind leaves. Chromatographic analyses (TLC, UV, MS, NMR, and IR) indicated the presence of putative caffeic acid derivative in Tamarind leaves. Aspergillus tamarii was highly sensitive to TrEAF compound at all concentrations (500 ppm, 1000 ppm, 1500 ppm and 2000 ppm) than Aspergillus fumigatus, Aspergillius niger. In Aspergillus tamarii and Aspergillius niger, 1500ppm concentration was very much sensitive to TrEAF compound. Cercina lutia, Staphylococcus aureus, Micrococcus roseus and Pseudomonas sp were much more sensitive to TrEAF compound than Escherichia coli. No effect of TrEAF compound was detected in E.coli at concentration of 1000ppm. When isolated and purified by fractionation and column chromatography, this active TrEAF compound showed concentration-dependent inhibitory/stimulatory activity on rice seeds. At concentration of 1000ppm, it showed 53.81% inhibition on shoot length and 58.85% inhibition in root length. At 500ppm concentration, 29.01% inhibition in shoot length and 38.82% inhibition in root length have been detected. Very slight stimulation on shoot length was observed at 7.81ppm. It showed no effect on the germination of rice seed.

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Oluremi, Adewale Ekundayo, and Ojokoh Anthony Okhonlaye. "Effects of Fermentation on the Antioxidant and Antinutritional Compositions of Green Pea." South Asian Journal of Research in Microbiology, August 11, 2020, 1–14. http://dx.doi.org/10.9734/sajrm/2020/v7i330171.

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Green peas are known to contain anti-nutritional factors like enzymes inhibitors, phytates, oxalates, saponins and polyphenolic compounds, all of which limit their utilization hence, the study evaluate the effect of fermentation on the antioxidant and antinutrients content of green pea. Fermentation of green pea was done using both submerged and solid state fermentation for 7days. Isolation and identification of microorganism from the fermented sample was done on daily basis using standard microbiological and molecular techniques. The type of organism isolated from the submerged fermentation of Green pea included the bacteria (Bacillus subtilis, Lactobacillus Plantarum, Micrococcus roseus, Lactobacillus lactis, and Lactobacillus fermentum) and the fungi Rhizopus oryzae, Penicillium chrysogenum and Rhizopus stolonifer. While the type of organism isolated from the solid state fermentation of Green pea included some bacteria (Bacillus subtilis, Lactobacillus Plantarum and Lactobacillus lactis) and fungi (Penicillium notatum, Rhizopus oryzae, Penicillium chrysogenum, Candida albicans, Alternaria alternate and Rhizopus stolonifer). Fermentation reduced the antinutritional content of the fermented sample with submerged fermentation resulting in the highest reduction from 32.18 mg/g, 4.14 mg/g, 1.62 mg/g, 51.08 mg/g and 36.37 mg/g in the raw sample to 26.27 mg/g, 0.48 mg/g, 0.27 mg/g, 7.82 mg/g and 24.07 mg/g in submerged fermented green pea for saponin, tannin, oxalate, phytate and alkaloid respectively. However, Fermentation significantly p ≤ 0.05 increased the phenol, flavonoid and FRAP content of the fermented green pea with the solid state fermentation resulting in the highest increase from 3.50, 0.03 and 1.41 in the raw sample to 9.32, 0.12 and 9.66 in the solid state fermented green pea for phenol, flavonoid and FRAP content respectively. This study revealed that fermentation had significant effect on the antioxidant and antinutritional compositions of Green pea thereby reducing the antinutrient composition of Green pea in which will improve the nutrient value of Green pea.

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Journal articles on the topic 'Micrococcus roseus'

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