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Journal articles on the topic 'Microdilution checkerboard titration procedure'

Author: Grafiati

Published: 3 June 2025

Last updated: 1 August 2025

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1

Dr., Muhammad Irfan Sharif Dr. Hammood ur Rehman Dr. Muhammad Danish. "A VIRTUAL INVESTIGATIONAL RESEARCH TO REGULATE THE ISSUES OF CURE DISAPPOINTMENT IN TYPHOID THROUGH ACTION AND NEW COMBINATION OF AQUEOUS GARLIC CUTTING AND CIPROFLOXACIN." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 11 (2018): 12700–12707. https://doi.org/10.5281/zenodo.1492765.

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<strong><em>Objective: </em></strong><em>To regulate developing a problem of cure disappointment in typhoid by assessing the action of the novel mixture of aqueous garlic cutting and ciprofloxacin. </em> <strong><em>Methodology:</em></strong><em> </em><em>This virtual investigational research was led in Services Hospital Lahore (November 2016 to August 2017). Synergism of garlic having ciprofloxacin in contrast to Typhi remained measured to resolve the serious matter of cure letdown having greatest identified anti-typhoid medicines till nowadays i-e fluoroquinolones. Twenty-six ciprofloxacin v

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Ángyán, Virág D., Viktória L. Balázs, Marianna Kocsis, et al. "Synergistic Antibiofilm Effects of Chestnut and Linden Honey with Lavender Essential Oil Against Multidrug-Resistant Otitis Media Pathogens." Antibiotics 14, no. 2 (2025): 146. https://doi.org/10.3390/antibiotics14020146.

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Background/Objectives: Bacterial resistance to antibiotics is a major problem in healthcare, complicated by the ability of bacteria to form biofilms. Complementary therapy for infectious diseases can rely on natural substances with antibacterial activity, e.g., essential oils and honeys. The aim of the study was to investigate the effects of linden and chestnut honeys, lavender essential oil, and their combinations against the multidrug-resistant otitis media pathogens Haemophilus influenzae, H. parainfluenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, and Streptococcus pneumoniae. The ef

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3

Ijaz, Saadia, Farheen Ansari, Muhammad Nawaz, et al. "Genomic Insights into and In Vitro Evaluation of Antimicrobial Combination Therapies for Carbapenem-Resistant Acinetobacter baumannii." Medicina 60, no. 7 (2024): 1086. http://dx.doi.org/10.3390/medicina60071086.

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Background and Objectives: Acinetobacter baumannii (A. baumannii), particularly carbapenem-resistant A. baumannii (CRAB), represents a grave concern in healthcare settings and is associated with high mortality. This study aimed to conduct molecular, mutational, and phylogenetic analyses of specific genes in CRAB and evaluate the synergistic effects of selected antimicrobial combinations. Materials and Methods: Phenotypic characterization was performed on six CRAB strains by using the Modified Hodge Test (MHT) and IMP-EDTA Double-Disc Synergy Test (IMP-EDTA DDST). Carbapenemase- and metallo-bet

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Krychowiak-Maśnicka, Marta, Mirosława Krauze-Baranowska, Sylwia Godlewska, et al. "Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants." International Journal of Molecular Sciences 22, no. 9 (2021): 4849. http://dx.doi.org/10.3390/ijms22094849.

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Carnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs) with secondary metabolites from selected carnivorous plant species. We employed the broth microdilution method, the checkerboard titration technique and comprehensive

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Szerencsés, Bettina, Mónika Vörös, Kristóf Bagi, et al. "Semi-Synthetic Ecdysteroid 6-Oxime Derivatives of 20-Hydroxyecdysone Possess Anti-Cryptococcal Activity." Microbiology Research 13, no. 4 (2022): 985–94. http://dx.doi.org/10.3390/microbiolres13040071.

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Cryptococcosis, a life-threatening fungal infection, frequently occurs in patients suffering from AIDS. The treatment of the disease is hampered by the limited number of the effective drugs and the increasing resistance; therefore, to find new active substances is needed. As meningitis is the most serious infection affecting the AIDS patients, effective drugs have to be capable of entering to the central nervous system. Ecdysteroids are natural bioactive molecules with considerable anabolic activity and without toxic side effects on humans. The aim of this work was to study the anti-cryptococc

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Badawy, Mona Shaban E. M., Omnia Karem M. Riad, Marwa F. Harras, Reem Binsuwaidan, Asmaa Saleh, and Samar A. Zaki. "Chitosan–Aspirin Combination Inhibits Quorum-Sensing Synthases (lasI and rhlI) in Pseudomonas aeruginosa." Life 14, no. 4 (2024): 481. http://dx.doi.org/10.3390/life14040481.

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Background: Quorum sensing (QS) controls the virulence of P. aeruginosa. This study aims to determine the anti-QS activity of aspirin alone and in combination with chitosan to reach maximum inhibition. We tested ten virulent Pseudomonas aeruginosa (P. aeruginosa) isolates and screened for N-acyl homoserine lactone (AHL) production using Agrobacterium tumefaciens as a biosensor. P. aeruginosa isolates were treated with sub-minimum inhibitory concentrations (MICs) of aspirin and chitosan–aspirin. We used broth microdilution and checkerboard titration methods to determine the MICs and the synergi

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Marques, M. B., E. S. Brookings, S. A. Moser, P. B. Sonke, and K. B. Waites. "Comparative in vitro antimicrobial susceptibilities of nosocomial isolates of Acinetobacter baumannii and synergistic activities of nine antimicrobial combinations." Antimicrobial Agents and Chemotherapy 41, no. 5 (1997): 881–85. http://dx.doi.org/10.1128/aac.41.5.881.

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The in vitro susceptibilities of 69 nosocomial Acinetobacter isolates were determined by the broth microdilution method. Fourteen (20%) isolates were resistant to at least two aminoglycosides and two extended-spectrum penicillins. Nine antimicrobial combinations were then tested for synergy against these 14 isolates by checkerboard titration: imipenem with ciprofloxacin, amikacin, and tobramycin and ampicillin-sulbactam, piperacillin-tazobactam, and ticarcillin-clavulanate with amikacin and tobramycin. Synergy was detected with one or more antimicrobial combinations against 9 of 14 (64%) isola

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8

Pietschmann, Jan, Holger Spiegel, Hans-Joachim Krause, Stefan Schillberg, and Florian Schröper. "Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection." Toxins 12, no. 5 (2020): 337. http://dx.doi.org/10.3390/toxins12050337.

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Food and crop contaminations with mycotoxins are a severe health risk for consumers and cause high economic losses worldwide. Currently, different chromatographic- and immuno-based methods are used to detect mycotoxins within different sample matrices. There is a need for novel, highly sensitive detection technologies that avoid time-consuming procedures and expensive laboratory equipment but still provide sufficient sensitivity to achieve the mandated detection limit for mycotoxin content. Here we describe a novel, highly sensitive, and portable aflatoxin B1 detection approach using competiti

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Adeniji, Oluwaseun Ola, Nolonwabo Nontongana, and Anthony Ifeanyin Okoh. "Prevalence of Class 1 Integron and In Vitro Effect of Antibiotic Combinations of Multidrug-Resistant Enterococcus Species Recovered from the Aquatic Environment in the Eastern Cape Province, South Africa." International Journal of Molecular Sciences 24, no. 3 (2023): 2993. http://dx.doi.org/10.3390/ijms24032993.

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Enterococci are regarded as a better indication of faecal pollution in freshwater and marine waters. Their levels in seawater are positively connected with swimming-related gastrointestinal disorders. This study used an Enterococcus-specific polymerase chain reaction (PCR) to characterize the isolates. Classes 1 and 2 integrons were examined for environmental Enterococcus isolates using a standard biological procedure. All strains were assessed against a panel of 12 antibiotics from various classes using disc diffusion methods. The microdilution method was used to work out the minimum inhibito

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Deepachandi, Bhagya, Sudath Weerasinghe, Himali Gunathilake, et al. "Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis." International Journal of Analytical Chemistry 2020 (July 15, 2020): 1–8. http://dx.doi.org/10.1155/2020/9289651.

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Human leishmaniasis which is considered a neglected tropical parasitic disease presents in three main clinical forms (i.e., cutaneous leishmaniasis (CL), mucocutaneous leishmaniasis (MCL), and visceral leishmaniasis (VL)) that are mainly determined by its causative species. Leishmania donovani, the most virulent and visceralizing parasite, is increasingly reported to cause CL in many countries in the world. Although CL is generally not considered to evoke a humoral immune response except for a nonrobust and a variable response in minority of cases, VL is associated with a clear strong humoral

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Barchiesi, Francesco, Anna M. Schimizzi, Francesca Caselli, et al. "Interactions between Triazoles and Amphotericin B against Cryptococcus neoformans." Antimicrobial Agents and Chemotherapy 44, no. 9 (2000): 2435–41. http://dx.doi.org/10.1128/aac.44.9.2435-2441.2000.

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ABSTRACT The interaction of amphotericin B (AmB) and azole antifungal agents in the treatment of fungal infections is still a controversial issue. A checkerboard titration broth microdilution-based method that adhered to the recommendations of the National Committee for Clinical Laboratory Standards was applied to study the in vitro interactions of AmB with fluconazole (FLC), itraconazole (ITC), and the new investigational triazole SCH 56592 (SCH) against 15 clinical isolates ofCryptococcus neoformans. Synergy, defined as a fractional inhibitory concentration (FIC) index of ≤0.50, was observed

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12

El-Demerdash, Azza S., Rania M. Orady, Ahmed A. Matter, and Amera F. Ebrahem. "An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken." Indian Journal of Microbiology, November 17, 2023. http://dx.doi.org/10.1007/s12088-023-01124-2.

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AbstractSurface-growing antibiotic-resistant pathogenic Salmonella is emerging as a global health challenge due to its high economic loss in the poultry industry. Their pathogenesis, increasing antimicrobial resistance, and biofilm formation make them challenging to treat with traditional therapy. The identification of antimicrobial herbal ingredients may provide valuable solutions to solve this problem. Therefore, our aim is to evaluate the potency of nano garlic as the alternative of choice against multidrug-resistant (MDR) Salmonella isolates using disc diffusion and microdilution assays. T

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Škovranová, Gabriela, Marie Čulenová, Jakub Treml, Lucia Dzurická, Ivana Marova, and Alice Sychrová. "Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing." Frontiers in Pharmacology 13 (November 30, 2022). http://dx.doi.org/10.3389/fphar.2022.1068371.

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Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study aimed to investigate the antibacterial potential of prenylated phenolics in wound healing. Phenolic compounds isolated from the root bark of Morus alba L. were investigated for their antistaphylococcal potential both alone and in combination with commonly used antibiotics. The minimum inhibitory con

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14

Samaddar, Arghadip, Jenevi Margaret Mendonsa, Sukanya Sudhaharan, et al. "In vitro evaluation of antifungal combinations against neurotropic dematiaceous fungi associated with primary cerebral phaeohyphomycosis." Microbiology Spectrum, June 26, 2024. http://dx.doi.org/10.1128/spectrum.00781-24.

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ABSTRACT Primary cerebral phaeohyphomycosis is a life-threatening disease caused by neurotropic dematiaceous fungi. At present, there are no consensus guidelines regarding optimal antifungal therapy in such cases. Generally, a combination of antifungal agents is recommended for treatment. However, the activities of antifungal combinations against these fungi have not been investigated. In this study, we evaluated the in vitro activities of 13 double and five triple antifungal combinations against clinical isolates of Cladophialophora bantiana ( n = 7), Fonsecaea monophora ( n = 2), and Cladosp

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15

Li, Wanqian, Yanru Feng, Hui Lu, and Yuanying Jiang. "S3.4a The repurposing approach identifies pitavastatin (calcium) making fluconazole fungicidal by inhibiting ergosterol synthesis." Medical Mycology 60, Supplement_1 (2022). http://dx.doi.org/10.1093/mmy/myac072.s3.4a.

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Abstract S3.4 Free oral paper session, September 21, 2022, 4:45 PM - 6:15 PM Objectives Making fluconazole (FLC) fungicidal in combination with adjuvants is a promising strategy to avoid the emergence of FLC resistance and eliminate the persistence and recurrence of fungal infections. To address this question, we combined in vitro screening of a library of FDA-approved drugs to identify compounds for making FLC fungicidal. Methods We performed a high-throughput screen of an FDA-approved compound library (HY-L022, MCE®), which contains 2372 drugs, to identify potentially novel FLC synergistic l

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16

"Evaluation of specific chicken IgY antibody value developing diagnostic capture antibody ELISA kit against Foot and Mouth disease." Archives of Razi Institute, June 25, 2023, 201–10. http://dx.doi.org/10.32592/ari.2024.79.1.201.

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The most preferred method for the detection of foot-and-mouth disease (FMD) viral antigen and identification of viral serotype is the ELISA. Diagnostic tests with high sensitivity is necessary both to distinguish infected vaccinated animals and disease control programs for identifying the carrier animals. To detection of FMD virus, the current strategies are mainly based on capture antibody (sandwich) ELISA test. Applying laying pullets as animal bioreactor for production specific egg yolk antibody (IgY) has increased in recent years due to its high yield, affinity, low price, and quick produc

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