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Rennie, Paul John. "The skin microflora and the formation of human axillary odour." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.246346.
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Farrell, Angela Margaret. "Staphylococcus epidermidis lipase : biochemical and molecular genetic analysis." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252636.
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Assis, Ananda Brito de. "Análise sobre a microbiota cutânea de anfíbios em fragmentos de floresta atlântica e sua eficácia contra agentes patogênicos." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/41/41135/tde-10052011-185744/.
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A pele dos anfíbios, assim como de outros animais, atua como primeira proteção contra agentes patogênicos. A comunidade microbiológica ali residente é composta de algumas espécies de bactérias, e estas, possuem ação antifúngica contra patógenos conhecidos, inclusive Batrachochytrium dendrobatidis (Bd), o suposto agente principal de declínios de populações de anfíbios em diversas partes do mundo. Uma vez que as variáveis químicas e físicas de um ecossistema influenciam o crescimento, sobrevivência e atividade metabólica dos microorganismos, a microbiota cutânea que atua como barreira de proteção nos anfíbios contra agente infecciosos, provavelmente é afetada quando determinados parâmetros ecofisiológicos são alterados em ambientes florestais fragmentados, modulando assim a vulnerabilidade das populações de anfíbios aos agentes patogênicos. Nossa pesquisa esteve focada na caracterização das comunidades microbianas residentes da pele dos anfíbios em dois contextos de paisagem: fragmento e área contínua. Os parâmetros utilizados para essas análises foram a densidade microbiana e a riqueza de morfotipos de colônias bacterianas. O potencial inibitório do crescimento de patógenos também foi testado em ensaios do tipo cross-strak. As diferenças de densidade e riqueza microbiana entre as paisagens e a presença de táxons típicos de ambiente, apontam para o ambiente como um componente importante na determinação dos perfis das comunidades microbianas dos anfíbios estudados. Essas mudanças são muito provavelmente conseqüências, mas para o entendimento da extensão e natureza de tais conseqüências são necessários estudos adicionais.The skin of amphibians, as well as that of other animals, acts as a first protection barrier against pathogens. The microbial community resident in the amphibian skin is composed of some species of bacteria that may have antibacterial or antifungal action against known pathogens, including Batrachochytrium dendrobatidis, the alleged principal agent Tleading to declines of amphibian populations around the world. Because the chemical and physical variables of the landscape influence the growth, survival and metabolic activity of microorganisms, the function of skin as a protective barrier against infectious agents in amphibians, is likely affected by parameters that are altered in fragmented forest habitats. Thus, it is important to understand how environmental conditions affect the skin microbiota of amphibians, and the possible induced changes on vulnerability of amphibians to pathogens. Our research aimed to characterize the microbial communities living skin of amphibians in two contexts of landscape: fragment and continuous area. The parameters used for this analysis were density and richness of microbial morphotypes of bacterial colonies. The potential inhibition of pathogen growth was also evaluated using a cross-streak test, and some taxa in these communities were identified using international protocols. The observed differences in microbial density and richness across landscapes, and the presence of bacterial taxa typical of given environments, point out to the role of environmental change as an important component determining the profiles of microbial communities living on the skin of amphibians. These changes are very likely consequential, but understanding the scope and nature of consequences require additional study.
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Benoist, Louis. "Etude du système immunitaire chez la seiche Sepia officinalis : un potentiel pour l'aquaculture Omic Analysis of the Sepia officinalis White Body: New Insights into Multifunctionality and Haematopoiesis Regulation In-Depth In Silico Search for Cuttlefish (Sepia officinalis) Antimicrobial Peptides Following Bacterial Challenge of Haemocytes." Thesis, Normandie, 2020. http://www.theses.fr/2020NORMC226.
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Les Céphalopodes comme la seiche Sepia officinalis, malgré une durée de vie courte, sont retrouvés dans les océans depuis des millions d’années. Chez ces animaux atypiques, peu de pathologies ont été observées révélant la présence d’un système immunitaire efficace mais peu étudié qui repose sur des processus innés. L’étude du système immunitaire de la seiche a été menée au niveau du corps blanc, organe hématopoïétique ; des cellules circulantes, les hémocytes et au niveau de la peau, première barrière avec le milieu extérieur. Au niveau du corps blanc, l’étude transcriptomique et protéomique a mis en évidence la présence de facteurs en lien avec l’hématopoïèse dont des membres de la voie de signalisation JAK-STAT. Des facteurs immunitaires ont également été identifiés révélant une possible multifonctionnalité du corps blanc. La réponse immunitaire face à Vibrio splendidus a pu être appréhendées à partir d’une analyse transcriptomique comparative sur les hémocytes. Toutefois cette dernière n’ayant pas permis d’identifier clairement des peptides antimicrobiens, une analyse in silico originale a été développée permettant de sélectionner cinq peptides candidats dont trois ont révélé une activité antibactérienne ciblée contre des bactéries du genre Vibrio. Enfin, une étude au niveau de la peau et de son mucus a été initiée. Cette étude par des approches -omiques a permis l’identification de facteurs en lien avec la reconnaissance des pathogènes et la réponse immunitaire. Par ailleurs, douze souches ont pu être isolées et identifiées au niveau du microbiome cutané. L’ensemble de ces résultats représente un apport majeur concernant le système immunitaire chez la seiche permettant d’initier des études fonctionnelles lors d’une infection ou en fin de vie. Ces études permettraient de comprendre le mode d’action des facteurs immunitaires identifiés, l’implication de chaque entité dans la réponse immunitaire ou dans la mise en place et la maintenance du microbiomeCephalopods such as the cuttlefish Sepia officinalis, despite their short lifespan, have been found in the oceans for millions of years. In these atypical animals, few pathologies have been observed, revealing the presence of an effective but little studied immune system based on innate processes. The study of the cuttlefish's immune system has been carried out on the white body, a haematopoietic organ; on the circulating cells, the haemocytes; and on the skin, the first barrier with the external environment. At the white body level, the transcriptomic and proteomic study highlighted the presence of factors linked to haematopoiesis, including members of the JAK-STAT signalling pathway. Immune factors have also been identified, revealing a possible multifunctionality of the white body. The immune response to Vibrio splendidus could be apprehended from a comparative transcriptomic analysis of haemocytes. However, as the latter did not allow the clear identification of antimicrobial peptides, an original in silico analysis was developed to select five candidate peptides, three of which revealed a targeted antibacterial activity against bacteria of the Vibrio genus. Finally, a study of the skin and its mucus was initiated. This study using -omic approaches enabled the identification of factors related to pathogen recognition and immune response. In addition, twelve strains were isolated and identified at the level of the skin microbiome. All these results represent a major contribution concerning the immune system in cuttlefish, making it possible to initiate functional studies during an infection or at the end of life. These studies would make it possible to understand the mode of action of the identified immune factors, the involvement of each entity in the immune response or in the establishment and maintenance of the microbiome
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Grillon, Antoine. "Borréliose de Lyme : rôle de l’interface cutanée et du microbiome dans la physiopathologie de la maladie." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ097/document.
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La maladie de Lyme est la maladie à transmission vectorielle la plus répandue de l’hémisphère Nord. La peau est un organe clef dans cette maladie, car c’est à cet endroit qu’interagissent les cellules de l’hôte, le pathogène, le microbiote cutané et le vecteur. Nous avons développé un modèle murin d’infection disséminée de borréliose de Lyme, qui nous a permis de développer une méthode spécifique de détection de protéines de Borreliella dans le tissu cutané murin par SRM-MS, pouvant aboutir à une méthode de diagnostic chez l’homme. Dans un deuxième temps, nous nous sommes intéressés aux relations entre le microbiote cutané, les cellules résidentes de la peau, kératinocytes et fibroblastes, avec Borreliella. Les sécrétomes de trois bactéries commensales, S. epidermidis, P. acnes et C. striatum possèdent un effet synergique sur l’expression de gènes inflammatoires par les kératinocytes et les fibroblastes. Les sécrétomes de P. acnes et de C. striatum se sont également montrés capables d’inhiber une partie de la réponse inflammatoire des kératinocytes, pouvant aider le pathogène lors de la transmission/dissémination précoceLyme disease is the most common vector-borne disease in the Northern Hemisphere. Skin is a key organ in the disease, since it is the key interface where host cells, pathogen, skin microbiota and vector interact early during pathogen transmission. We developed a late lyme borreliosis model on mice. This model allowed us to develop a specific detection method of Borreliella proteins in the mouse skin by SRM-MS that might be used to develop a human diagnosis of disseminated Lyme disease. In a second part, we analysed the relationship between skin microbiota, resident skin cells (keratinocytes and fibroblasts), in the presence or absence of Borreliella. The secretome of three commensals bacteria, S. epidermidis, P. acnes and C. striatum was shown to have a synergistic activity with Borreliella in pro-inflammatory gene expressions by keratinocytes and fibroblasts. P. acnes and C. striatum secretomes were also able to inhibit partially the inflammatory response of keratinocytes that might help the transmission/dissemination of the pathogen
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Whitley, Elizabeth. "The microflora of Blue Stilton cheese." Thesis, University of Nottingham, 2002. http://eprints.nottingham.ac.uk/28538/.
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Blue Stilton is a blue-veined cheese manufactured in a restricted area of the UK, using lactic starter cultures plus a secondary culture of Penicillium roquefotti. The aim of this study was to determine the change in microflora during ripening of the cheese and to investigate potential microbial interactions. Additionally, the volatile compounds present in mature samples of cheeses exhibiting few blue veins were compared with those in good quality cheeses, showing ample blue veining. Experiments on cheeses from a single dairy, monitored during the ripening process, showed that the total Lactobacillus count increased from levels of around 103 cfu g-1 on day one to around 107 cfu g-1 after 8 weeks of ripening. This is comparable to values found in other cheeses including both mould-ripened and non mould-ripened varieties. Yeast counts were generally higher than those found in other cheeses and also increased to levels in the region of 107 g-1. The total viable count (TVC) decreased from around 109 g-1 initially, reflecting the presence of the starter bacteria, to 107 g.1, suggesting a decline in the starter bacteria similar to that found in other cheeses. Mature cheeses always exhibited similar numbers of microorganisms although the species varied between cheeses. High quality, mature, cheeses were compared with sub-standard cheeses from the same production site. The predominant species of lactobacilli in good quality cheeses were Lb. plantarum and Lb. curvatus, whereas in poor quality cheeses Lb. brevis predominated. This corresponded to the results of gas chromatography-olfactometry, which indicated the presence of fruity off flavours in poor quality cheeses. Several strains of these species were isolated, as indicated by differing capabilities in utilisation of a range of carbon sources. Yeast species also varied between good and poor quality cheeses with Candida sphaerlca and C. catenulata predominating in good cheeses and C. famata, C. lipolytica and C. catenulata also occurring in both good and poor quality samples. Strain differences were observed by the biochemical profiles and two strains of C. famata demonstrated inhibitory effects against P. roqueforti when incubated under anaerobiosis. It was concluded that these strains may affect the development of blue veins in Stilton cheese when maturation conditions encourage their proliferation. Comparisons were made between samples of cheeses from several Stilton producers and the results suggested that although the levels of the groups of microorganisms tested were similar, the species of lactobacilli and yeasts present were different. This suggests that the indigenous microflora may have a significant impact on the flavour of cheeses from individual production sites. It was concluded that the microflora of Blue Stilton cheese may have a significant impact on the quality of the product both in terms of flavour and the development of the blue veins.
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Andrade, Ana Margarida Dias Santos de Azevedo. "Microflora Intestinal: uma Barreira Imunológica Desconhecida." Dissertação, Instituto de Ciências Biomédicas Abel Salazar, 2009. http://hdl.handle.net/10216/52784.
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Andrade, Ana Margarida Dias Santos de Azevedo. "Microflora Intestinal: uma Barreira Imunológica Desconhecida." Master's thesis, Instituto de Ciências Biomédicas Abel Salazar, 2009. http://hdl.handle.net/10216/52784.
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Dall'Osso, Nicolo'. "Caratterizzazione della microflora degradativa di salsiccia fresca." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2017. http://amslaurea.unibo.it/14587/.
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La salsiccia fresca è un prodotto costituito da carne macinata, grasso suino, spezie, acqua, sale e zuccheri in bassa percentuale, con una rilevante importanza economica sul mercato nazionale ma per il quale, viste le caratteristiche compositive, la limitata shelf-life costituisce un limite per la commercializzazione. Essa dipende essenzialmente dai microrganismi presenti nella materia prima e, per poter arrivare all’applicazione di diverse strategie atte ad incrementare la shelf-life del prodotto e a controllare l’evoluzione di questa microflora, sono indispensabili dati più precisi circa il microbiota che caratterizza i processi degradativi.
Questo elaborato si inserisce in un più ampio progetto per la valutazione di questi aspetti ed è focalizzato sull’evoluzione del microbiota in diversi lotti prodotti industrialmente e conservati a temperature di 4°C e in abuso termico. Lo studio effettuato con tecniche microbiologiche convenzionali, tecniche analitiche mirate ad aspetti compositivi e studi metatassonomici ha consentito una buona definizione della variabilità che caratterizza il microbiota degradativo delle salsicce fresche poiché ciascun lotto era caratterizzato da un proprio pattern degradativo. In tutti i lotti però (tranne uno) esistono alcune popolazioni prevalenti quali L. sakei, Leuconostoc e B. thermosphacta. Un unico lotto presentava un pattern degradativo difforme dagli altri con le specie dominanti riconducibili alle Enterobacteriaceae.
Questo elaborato ha consentito di chiarire meglio quello che è la potenziale evoluzione del microbiota delle salsicce fresche in rapporto alla loro shelf-life. I risultati hanno mostrato una certa variabilità all’interno di un pattern abbastanza definito, ma hanno anche evidenziato la possibilità di eventi non controllati che alterano in maniera significativa (e potenzialmente rischiosa) questo pattern.
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Gkatzionis, Konstantinos. "Flavour production of Stilton blue cheese microflora." Thesis, University of Nottingham, 2010. http://eprints.nottingham.ac.uk/11395/.
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In the blue cheese Stilton the starter mould Penicillium roqueforti grows and sporulates during the ripening period and is considered to be responsible for the unique blue cheese aroma. However, the sporulation of the mould, which results in the formation of blue veins, takes place in a fraction of the Stilton matrix which overall is very heterogeneous. Most blue cheeses develop a secondary microflora of yeasts which may affect their aroma. The aim of this study was to investigate the yeast flora of Stilton, the aroma profile of the cheese and the role of the yeasts in the aroma production. The approach in this work was to study individually the different sections of Stilton (the blue veins, the white core and the outer crust) as previous studies have demonstrated each section has a differing bacterial flora. In addition to the classical microbiology, a series of molecular techniques (Denaturing Gradient Gel Electrophoresis, Restriction Fragment Length Polymorphism and Terminal RFLP) were compared and applied for the screening of the local fungal communities in the cheese. The results showed that the two approaches were complementary. It was concluded that the structure of the fungal community was different for each section of the cheese. The aroma profiles of the three different sections of Stilton were studied using solvent extraction Gas Chromatography-Mass Spectrometry (GC-MS), a headspace GC-MS technique (SPME GC-MS) and direct headspace analysis (Atmospheric Pressure Chemical Ionisation [APCI]-MS). The different sections of Stilton presented different aroma profiles. Overall, the blue and the outer crust had similar profiles. These two sections contained higher amount of ketones while the white contained higher amounts of alcohols and aldehydes. Yeast isolates and the starter Penicillium roqueforti were cultivated alone and in combination in a cheese model and the aroma production was studied with SPME GC-MS analysis. The co-culture of the starter Penicillium roqueforti and individual yeast isolates resulted in aroma profiles different from those that were produced by the mould or the yeasts individually. The model of Penicillium roqueforti with Yarrowia lipolytica resulted in an aroma more similar to blue cheese than produced by the mould alone. Sensory analysis (Flash profile technique) was used in order to compare the aroma of this model with the aroma of blue cheeses and the perception of the combined culture was found to be similar to Stilton cheese, whereas that of the mould alone was not. Yeasts are a significant part of the microflora of Stilton and they are able to affect the aroma production. Selected isolates of Yarrowia lipolytica could be used in combination with Penicillium roqueforti for the production of blue cheese aroma e.g. as a starter culture.
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Suau, Antonia. "Molecular characterisation of the human gut microflora." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367720.
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Sheehy, Evelyn Catherina. "The oral microflora of children undergoing liver transplantation." Thesis, King's College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300430.
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Perez, Horácio Joaquín. "Distribuição topográfica da microflora intestinal em rato Wistar." reponame:Repositório Institucional da UFSC, 2014. https://repositorio.ufsc.br/xmlui/handle/123456789/123202.
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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Ciências Médicas, Florianópolis, 2014.Made available in DSpace on 2014-08-06T17:55:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2014
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JESUS, CRISTIANE FATIMA DIAS DE. "SKIN DISORDERS: ONE SKIN FOR TWO?" PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2004. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=5226@1.
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COORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIORA pele tem uma importância fundamental na nossa constituição psíquica. Através dos primeiros contatos de pele com a mãe ou ambiente maternante o bebê começa a formar as mais primitivas impressões acerca do corpo que lhe pertence e do mundo que o rodeia. Este estudo pretende mostrar que as afecções de pele refletem um desejo de retorno ou permanência no estado de indiferenciação com a mãe. Deste modo, parte-se do princípio que distorções no elo mãe-filho desde cedo não permitem ao mesmo vivenciar com naturalidade seu trajeto rumo à independência, nos termos de Winnicott, ou em direção à individuação, de acordo com Mahler. A falta de um ambiente bom o bastante dificulta a aquisição de uma experiência subjetiva de corpo. Por outro lado, abordam- se também os pressupostos da Escola de Psicossomática de Paris, segundo a qual, os processos que levam à vulnerabilidade psicossomática também estariam relacionados à falhas na estruturação desta ligação, resultando na insuficiência e/ ou desorganização das funções psíquicas. Portanto, com este objetivo pretende-se mostrar que as afecções de pele refletem uma falha nos processos de delimitação das fronteiras do corpo pela falta ou inadequação do investimento materno nos primeiros anos de vida, dificultando a experiência de unidade e coesão de seus processos internos e externos. Na parte final, procede-se à discussão de um caso clínico à luz das teorias anteriormente mencionadas.
The skin is of basic importance to our psychic constitution. Through the first skin contacts with the mother or mothering environment the baby starts to form the most primitive impressions concerning the body that belongs to him/her as well as the world that surrounds it. This study intends to show that skin disorders reflect the desire of a return or permanence in the state of indiferenciation with the mother. In this way, based on distortions in the early mother-baby link, the child is not allowed to naturally live his passage towards independence, as per Winnicott, or towards individuation, according to Mahler. The lack of a good enough environment makes the acquisition of a subjective body experience difficult. On the other hand, principles of the school of psychoanalytic psychosomatics of Paris were used, according to which processes that lead to the psychosomatic vulnerability would also be related to imperfections in structuring this relationship, which would result in an insufficient and/or a disorganization of the psychic functions. Therefore with this objective in mind, it was intended to show that skin disorders reflect a failure in the process of delimitation of the body limits due to the lack or inadequate maternal investments in the first years of life, hindering an experience of oneness and cohesion of internal and external processes. In the final part of the dissertation a clinical case is discussed according to the theoretical background covered in this work.
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Pan, Wei. "Skin image processing and skin characterizations." Thesis, London South Bank University, 2017. http://researchopen.lsbu.ac.uk/1847/.
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The skin hydration and skin Trans epidermal water loss (TEWL) are of great importance in many skin research areas, such as dermatology, clinical analysis, pharmacology and cosmetic science etc. However, to measure them is not easy. Over the year , our research group has developed three novel technologies for such measurement : Opto Thermal Transient Emission Radiometry (OTTER), AquaFlux and capacitive contact imaging based on the Fingerprint sensor. The aim of this research is to develop new skin image processing and data analysis techniques for capacitive contact images, as well as digital colour images, and to develop new methodologies for skin characterization by using the three technologies. For skin image processing, a new GUI based MATLAB programme has been developed, which can be used for extracting and analysing the images from the result files created by the measurement instruments. The programme implement the skin image processing techniques such as image enhancement (i.e. brightness equalization, extraction of skin texture, hair removal), image stitching, image matching and skin surface 3D profiling etc. Another image processing programme based on OpenCV has also been developed, which is more suitable for real time video processing, including contour detection, texture extraction and face detection etc. For the skin characterization, several experiments are conducted: skin over hydration experiments; kin damage assessment including intensive washing, SLS irritations, and tape stripping; dermabrasion experiments; soap drying effect assessment. These experiments provide better understandings of the technologies. The occlusion effects in capacitive images shows good potential for skin damage assessment, as it can not only reflect the scale of damage, but also the types of damage.
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Johansson, Birgit. "Bad breath : prevalence, periodontal disease, microflora and inflammatory markers /." Huddinge, 2005. http://diss.kib.ki.se/2005/91-7140-295-0/.
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Healy, Rosemary Edith. "Effects of the microflora of barley on malt characteristics." Thesis, Heriot-Watt University, 1985. http://hdl.handle.net/10399/1627.
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Baldwin, Neil A. "The effect of foliar fungicides on cereal phylloplane microflora." Thesis, Liverpool John Moores University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292609.
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Inness, Vicky Leanne. "The canine and feline microflora : the application of prebiotics." Thesis, University of Reading, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414614.
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Gobbi, Nicolas. "Caratterizzazione della microflora spontanea di un alimento fermentato vegano." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/9384/.
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Tra i prodotti vegani più richiesti vi sono i “formaggi” vegani, alimenti fermentati a base di frutta secca o ottenuti attraverso trattamenti su latte di mandorla e latte di soia, e successivamente fermentati. Nella mia attività ho caratterizzato un alimento fermentato vegano e studiato la successione microbica durante la fermentazione di un “formaggio” ottenuto partendo da anacardi e preparato in maniera artigianale. Oltre agli aspetti microbiologici, ho analizzato anche alcune caratteristiche fisico chimiche. Durante il processo di produzione gli anacardi vengono messi in ammollo per 8 ore a temperatura ambiente e, successivamente, i semi vengono scolati e risciacquati sotto acqua corrente. Gli anacardi vengono poi addizionati di acqua e microrganismi probiotici e tritati in un mixer fino al raggiungimento di una crema omogenea. A questo punto il prodotto viene lasciato riposare a temperatura ambiente per 48 ore durante le quali ha luogo la fermentazione e poi addizionato di ingredienti. Le indagini chimico fisiche effettuate hanno evidenziato che il pH si mostra già basso prima dell’inizio della fermentazione vera e propria e scende a 4.5 dopo 48 ore di riposo a causa dell’accumulo di acidi organici, ed in particolare di acido lattico e acetico che indicando un’attività fermentativa condotta dai batteri lattici. Le analisi microbiologiche hanno confermato che l’effettivo agente di fermentazione era costituito da questi batteri che sono stati identificati a livello molecolare. Le specie identificate due eterofermentanti (Weissella e Leuconostoc), presenti soprattutto nelle prime fasi della fermentazione, ed una omofermentante (Pediococcus), prende il sopravvento mano a mano che la fermentazione procede. Il lavoro svolto ha permesso di ottenere alcune importanti informazioni per la produzione industriale di un “formaggio” vegano fermentato. Il processo studiato presenta numerosi punti di rischio che devono essere presi in considerazione prima di poter giungere alla messa a punto di un prodotto definitivo.
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Semenyak, A. V. "Peculiarities of pregnancy in the presence of opportunistic microflora." Thesis, БДМУ, 2022. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/19739.
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Bederski, Hans Jurgen 1966. "Adaptation of goat rumen microflora and microfloral activity of non-adapted cow versus goat microflora to tannin rich shrub live oak (Quercus turbinella)." Thesis, The University of Arizona, 1991. http://hdl.handle.net/10150/278019.
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Tannins are polyphenolic compounds which precipitate, or conjugate, with proteins to make them indigestible. Tannins appear to confer anti-herbivore defense in shrub live oak (Quercus turbinella), a common browse in the interior chaparral of Central Arizona. The results of the present study indicate that the rumen microflora in goats can adapt to tannin rich diets thereby increasing in vitro dry matter digestibility of shrub live oak leaves. Major digestion inhibitors of shrub live oak appear to be fiber content (NDF, ADF, and lignin) and presence of condensed tannins in the leaves. In vitro dry matter digestibility of oak leaves appears to be higher in rumen microflora from goats than in rumen microflora from cows, although further research is needed to determine digestive efficiency among these species.
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George, Maryan. "Adrenaline releases level on skin-to skin touches." Thesis, Högskolan i Skövde, Institutionen för hälsovetenskaper, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-19090.
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Human pleasant touches promote feelings of security, supportiveness, and wellbeing. Conversely, human unpleasant touches promote the body for either “fight or flight” or “short term acute stress” during emergencies, feeling of stress or danger. The promoted stress response is released from the hypothalamus by the sympathetic nerve system further to the spinal cord to reach the signals to the adrenal medulla, where stress hormones adrenaline is released. Adrenaline, which is characterized by a mimic sympathetic nerve system, interacts with α and β receptors on different organs. The aim for this study was to investigate whether the stroker (partner/stranger) touch effects on adrenaline hormone releases. The null hypothesis for this study entails a significant adrenaline reduction in partners’ touches compared with strangers’ touches. Indirect competitive ELISA method was used, and concentration data of a total of sixteen participants was obtained. Whitney-U test was carried out to compare group differences within stroker (stranger/partner) touches and adrenaline releasing level. In addition, correlation in adrenaline with noradrenaline and oxytocin hormones was obtained using Spearman’s correlation test. The significant p-value 0.05 was conducted. The result of this study showed no differences between stroker (partner/stranger) associated with adrenaline hormone release. Correlation between partner maximum (max) concentration data for both oxytocin and adrenaline had significant differences. However, max variables for adrenaline and noradrenaline within stroker did not show significant differences. The conclusion of this study is that the gentle touch stimulus used in this study was not enough to detect stress hormone in adrenaline.
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Lutz, Monica Ann. "Skin-to-Skin Bonding and Cesarean Section Delivery." Kent State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=kent1368115929.
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Meade, Celia. "Skin boat." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0002/MQ34931.pdf.
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Orth, Margaret A. (Margaret Ann) 1964. "Skin flicks." Thesis, Massachusetts Institute of Technology, 1993. http://hdl.handle.net/1721.1/69305.
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Thesis (M.S.V.S.)--Massachusetts Institute of Technology, Dept. of Architecture, 1993.Includes bibliographical references (leaves 100-102).
The written and artistic part of this thesis are both separated into the two categories of "SKIN" and "FLICKS". The Artistic part of my thesis consists of five artificial skins made on my body, and a series of video tapes of the performance of me making those skins. A number of devices have been built into these skins to provide my body and self with messages and stimuli from the outside. Through the action of rebuilding myself and exposing some of the elements that form me I am physically and materially exploring the nature of subjectivity and constructed self in our society. The video tapes of the performance are representations of both the act of rebuilding myself and my body. They are reflections and criticism of the way women are taught to see and present themselves in our culture. They are the product of my perspective and of a culture of surveillance, pornography and empirical observation. The written part of my thesis deals with the sanctity of making through the two categories of "SKIN" and "FLICKS". The "SKIN" part consists of analysis of the physical properties of skin, its ability to be imitated and its role in material making. It relates our attempt to recreate ourselves to the "rebuilding" of the natural world. It explores through the physical material skin, the value and purpose of material making. The "FLICKS" section of my document explores and examines some of the history of the representation of skin in visual art. This history includes some classical works, Renaissance works, nineteenth century nudes and contemporary films. It also questions the ability of images to represent anything other than skin through a discussion of iconoclasm and its relation to some contemporary critical practices in the arts.
by Margaret Orth.
M.S.V.S.
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Gentry, Retha D., Lisa Ousley, and Candice N. Short. "Skin Examination." Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/etsu-works/7148.
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Bielak, Britta. "Second Skin." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3583.
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Reason for writing. The space of confusion and possibility where the practices of art and design collide seems to be in a constant amoebic state. This place of shared influence and growth seems to pervade not only the intersection of these two disciplines, but within interior design, the intersection of people and space. How can the boundaries between an interior space and it’s inhabitants be as richly embedded with tension and opportunity as the edges where art and design meet? Like art and design, how can a space and it’s visitors interact to affect one another?
Problem + Methodology: This project explores these questions in a context mindful of their origin: The Virginia Museum of Fine Arts. The design proposal of inserting a fashion wing into the VMFA’s existing context evolves from research and process work across art, design, and architecture, from the scale of the building to the scale of a seat.
Results + Implications: The challenge of creating public space that can be just as responsive to and influential over it’s inhabitants as private space seems resolved through the navigation of movement and moment. Finding value in an unscripted discovery of a space and the ownership of private experiences, offers a way to feel engaged with and connected to a space that doesn’t rely on object ownership or territorial comfort. This solution does rely, however, on inhabitants capable of being present and responsive to their environment, allowing other visitor’s interactions with the space and their individual path through the exhibits to affect their perceptions of and connectedness with the design.
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Hughes, Claire Helen. "Influence of ionising radiation on the microflora of poultry meat." Thesis, Queen's University Belfast, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334566.
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Barcenilla, Adela. "Diversity of the butyrate-producing microflora of the human gut." Thesis, Robert Gordon University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310425.
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Bradshaw, D. J. "Continuous culture modelling of environmental factors affecting the oral microflora." Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315369.
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Grewal, H. K. "Enzymatic reduction of sulphoxide-containing xenobiotics by the intestinal microflora." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242185.
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Mosca, Rodrigo Crespo. "Inibição do crescimento da microflora oral por venenos de serpentes." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-16102009-150801/.
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A saúde bucal, na maioria dos municípios brasileiros, constitui ainda um grande desafio aos princípios do Sistema Único de Saúde, principalmente no que se refere à universalização, à eqüidade do atendimento e alto custo envolvido na terapia restauradora. A procura pela descoberta de novos compostos metabólicos com atividade antibacteriana para a prevenção de doenças bucais e talvez com menores impactos a saúde e financeiros, seria muito importante para obtenção de um meio efetivo de controle da formação de um biofilme patogênico e da cárie dental. O objetivo deste trabalho é estudar a viabilidade biotecnológica do uso de venenos nativos de diferentes serpentes quanto à capacidade de inibir o crescimento de Streptococcus mutans, principal agente envolvido na cárie dental. Nossos resultados mostraram que os venenos das serpentes Bothrops moojeni e Bothrops jararacussu inibiram o crescimento de Streptococcus mutans e o componente responsável pela inibição parece ser a peróxido de hidrogênio. Apesar de ainda não totalmente conclusivos, os ensaios já realizados, permitem afirmar que venenos de serpentes são ferramentas importantes na inibição do crescimento de patógenos, especificamente daqueles envolvidos nas doenças cariogênicas.The oral health at the most of Brazilian municipalities is still a big challenge to the principles of the Brazilian Health Unique System (SUS), particularly with regard to the globalization, the equity of care and high cost involved in restorative therapy. The demand for discovery of new natural products with antibacterial activity in order to prevent dental diseases and perhaps with fewer health and financial impacts, would be very important to achieve an effective means to control the formation of a pathogenic biofilm and dental caries. The objective of this work is to study the feasibility of the use of different snakes crude venom in inhibiting the growth of Streptococcus mutans, the principal agent involved in dental caries. Our results showed that Bothrops moojeni and Bothrops jararacussu venoms were able to inhibit the growth of Streptococcus mutans and the component responsible for that inhibition appears to be the hydrogen peroxide. Though still not fully conclusive, the tests already carried out, show that snake venoms are important tools to inhibit the growth of pathogens, specifically those involved in caries diseases. MOSCA, R.C., 2008 7 SUMÁRIO 1. INTRODUÇÃO................................................................................................
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MOSCA, RODRIGO C. "Inibição do crescimento da microflora oral por venenos de serpentes." reponame:Repositório Institucional do IPEN, 2008. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11707.
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Made available in DSpace on 2014-10-09T12:54:57Z (GMT). No. of bitstreams: 0Made available in DSpace on 2014-10-09T14:07:06Z (GMT). No. of bitstreams: 0
Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
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Edwards, Joan E. "Characterisation of the effect of flavomycin on the rumen microflora." Thesis, University of Aberdeen, 2003. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU602317.
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Flavomycin is a phosphoglycolipid antibiotic, which is used exclusively as a growth- promoting feed additive. Existing data, from both in vitro and in vivo ruminal studies, give conflicting results regarding its mode of action, as well as no clear microbiological basis for the observed responses. Studies however do indicate that the principal site of action of the antibiotic is the rumen. From the available data, flavomycin appears to promote growth in a manner distinct from that of other feed antibiotics, for which the growth-promoting mechanisms have been elucidated. This study aimed to characterise the effect that flavomycin has on the microflora of the rumen, allowing its growth promoting mechanism in ruminants to be determined. In vitro analysis demonstrated that flavomycin has only antibacterial activity, as ruminal species of protozoa, fungi and archaea were unaffected by the antibiotic. Of the ruminal bacterial species tested, Fusobacterium necrophorum, Fibrobacter spp. and certain hyper-ammonia producing (HAP) bacteria (Atopobium oviles, Desulfomonas sp. and Peptostreptococcus anaerobius) were highly sensitive to the antibiotic. The sensitivity of the Fibrobacter spp. to flavomycin suggested that flavomycin is likely to select for a cellulolytic bacterial flora comprised predominantly of Ruminococcus spp., as has been previously proposed on the basis of in vitro fermentation studies. In vivo, suppression of ruminal numbers of F. necrophorum and flavomycin sensitive HAP bacteria occurred as a result of flavomycin supplementation. It was demonstrated that these bacterial populations were highly variable, between individual sheep and days respectively, suggesting why previous studies produced conflicting results. Assessment of ruminal fermentation parameters demonstrated that flavomycin caused a significant decrease in the production of ruminal ammonia, which could be directly attributed to decreased numbers of ruminal HAP bacteria. A small increase in the ruminal concentration of lactate also occurred, which con-elated with the suppression of ruminal numbers of the lactate utilising F. necrophorum. No change in the balance of individual volatile fatty acids (VFA) occurred, however total VFA production was significantly decreased. This was likely to be due to the total viable anaerobic bacterial counts being lower during flavomycin supplementation, although this result was not statistically significant. Uncultured rumen bacteria were also implicated in the growth promoting mechanism of flavomycin. Molecular investigation of the rumen bacterial population by denaturing gradient gel electrophoresis (DGGE) demonstrated that several changes occurred, which correlated with flavomycin supplementation. Analysis of the sequence data obtained from excised DGGE bands highlighted that the majority of the operational taxonomic units (OTU) detected were represented by presently uncultured species of bacteria, of which almost half had not been previously identified. Identification of the flavomycin sensitive bacterial populations was not possible, however, due to the recovery of multiple sequences from individual DGGE bands. Existing bacterial 16S rDNA sequence data, from published ruminal clone libraries, also demonstrated the poor cultural representation of rumen bacterial diversity, with only 10% of the OTU detected being represented by cultured bacterial species. Based on these results, flavomycin has the ability to increase the efficiency of dietary protein utilisation, although the role of uncultured bacteria in the growth promoting mechanism of the antibiotic is not clear. Protein retention in the rumen is increased as a consequence of decreased deamination by ruminal HAP bacteria. F. necrophorum has the ability to attach to and damage rumen epithelium, as well as being the principal aetiological agent in the development of liver abscesses. Suppression of F. necrophorum is likely to decrease metabolic and immune burdens within the animal, as well as potentially reducing the rate of rumen wall tissue turnover. The use of flavomycin as a feed additive is to be banned in Europe in 2005. However, it is not known if presently available feed additives or treatments will be able to act as an effective replacement for this antibiotic. Characterisation of an adaptive resistance mechanism against flavomycin, in the ruminal bacterium Prevotella bryantii, demonstrated that cross-resistance to therapeutic antibiotics can occur. As a result of this finding, and the interest in development of phosphoglycolipid antibiotics for therapeutic application, it can be concluded that the withdrawal of the use of a flavomycin as a feed additive is a wise precautionary measure to ensure the long-term efficacy of this class of antibiotics.
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Larocque, Catherine. "Are We on the Same Page About Skin-to-Skin Care? A Descriptive Correlational Study Exploring Skin-to-Skin Care for Postoperative NICU Infants." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41079.
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Family-centered care (FCC) is considered the gold standard for care delivery in the Neonatal Intensive Care Unit (NICU). However, there are challenges with the implementation of FCC in practice and there is limited literature about how to tailor this approach for specialized NICU populations.
To explore FCC for surgical neonates in the NICU, the concept was explored using Roger’s evolutionary concept analysis. Results illustrate that FCC in the NICU is a philosophy or care, rather than a set of interventions. The subsequent cross-sectional descriptive exploratory study showed that the surgical infants in our sample (n=11) received a limited amount of skin-to-skin care (median 0 mins/day) and parents reported challenges to being involved in their infant’s care.
This thesis supports the challenges with the implementation of FCC in practice and both the need to consider multiple perspectives and the need for broader systemic change in order to support a FCC philosophy.
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Bertaux, Emilie. "Mechanical friction between skin and sport textiles yielding skin irritation." Valenciennes, 2008. https://ged.uphf.fr/nuxeo/site/esupversions/808ac959-0f92-473f-b3bb-2a0acab897a8.
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Afin d'améliorer le confort des coureurs en réduisant l'irritation de la peau due au phénomène de friction mécanique, le Textile Friction Analyzer a été récemment développé au sein de l'Empa. Cet appareillage permet de caractériser la friction peau-textile (T-shirt et chaussette) en utilisant une peau artificielle (skin model) et ceci en simulant les conditions de contacts durant la pratique sportive. Différentes peaux artificielles ont été développées afin de reproduire les propriétés frictionnelles de la peau du pied ou du téton humain. Les paramètres de tests comme la force de contact, la vitesse relative et le nombre de cycles propres à chaque textile et type d'activité sportive ont été définis par la réalisation d'études biomécaniques. Une autre étape importante a consisté au développement d'un système de chauffage pour la peau artificielle et la simulation de la sudation. Ces conditions spécifiques permettent donc la simulation de la friction mécanique entre la peau et les vêtements sportifs du type chaussette et T-shirt proche de la réalité. Le Textile Friction Analyzer a été utilisé pour étudier l'influence des fibres et des constructions sur la friction. De plus, des tests avec sujets ont été réalisé afin d'analyser les relations entre les paramètres textiles, physiologiques et sensoriels basés sur le confort durant la pratique sportive. Les résultats obtenus représentent une avancée dans la compréhension de l'influence des différents paramètres sur l'irritation de la peau durant l'activité sportive et peuvent être utilisés ultérieurement pour le développement de vêtements sportifs à faible coefficient de friction afin d'améliorer le confort des sportifsIn order to improve runners' comfort by reducing skin irritation due to mechanical friction, a Textile Friction Analyzer was previously developed at Empa. This measurement device characterizes the friction of socks and T-shirts against mechanical skin models under cyclic contact conditions which are typical for sport activities. Different skin models were developed in order to simulate the frictional properties of human foot skin or the human nipple. Testing parameters such as contact pressure, sliding velocity and number of friction cycles depend on the specific textile and sport application and were defined on the basis of biomechanical studies using pressure sensors and high speed cameras. Another important step was to develop a heating system for the skin models used on the Textile Friction Analyzer and to simulate the sweating of the human body. The specified testing conditions allow simulating cyclic friction contacts between sport textiles such as socks and T-shirts and skin close to reality. The Textile Friction Analyzer was used to study the influence of fibers and constructions on friction. Moreover, subjective tests were carried out to investigate the relationships between textile, physiological and sensorial parameters on comfort during sport activity using principal component analysis and decision trees. The results of this research represent a step towards a better understanding of the influence of different parameters on skin manifestations during sport activity which can be used for developing low friction sport textiles to increase sportsmen's comfort
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Karsten, Aletta Elizabeth. "The effect of skin phototype on laser propagation through skin." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/24272.
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The use of lasers for diagnosis and treatment in medical and cosmetic applications is increasing worldwide. Not all of these modalities are superficial and many require laser light to penetrate some distance into the tissue or skin to reach the treatment site. Human skin is highly scattering for light in the visible and near infrared wavelength regions, with a consequent reduction of the fluence rate. Melanin, which occurs in the epidermis of the skin, acts as an absorber in these wavelength regions and further reduces the fluence rate of light that penetrates through the epidermis to a treatment site. In vivo fluence rate measurements are not viable, but validated and calibrated computer models may play a role in predicting the fluence rate reaching the treatment site. A layered planar computer model to predict laser fluence rate at some depth into skin was developed in a commercial raytracing environment (ASAP). The model describes the properties of various skin layers and accounts for both the absorption and scattering taking place in the skin. The model was validated with optical measurements on skin-simulating phantoms in both reflectance and transmission configurations. It was shown that a planar epidermal/dermal interface is adequate for simulation purposes. In the near infrared wavelength region (676 nm), melanin (consisting of eumelanin and pheomelanin) is the major absorber of light in the epidermis. The epidermal absorption coefficient is one of the required input parameters for the computer model. The range of absorption coefficients expected for typical South African skin phototypes (ranging from photo-sensitive light skin, phototype I on the Fitzpatrick scale, to the photo-insensitive darker skin phototype V) was not available. Non-invasive diffuse reflectance spectroscopy measurements were done on 30 volunteers to establish the expected range of absorption coefficients. In the analysis it became apparent that the contributions of the eumelanin and pheomelanin must be accounted for separately, specifically for the Asian volunteers. This is a new concept that was introduced in the diffuse reflectance probe analysis. These absorption coefficient measurements were the first to be done on the expected range of skin phototypes for the South African population. Other authors dealing with diffuse reflectance probe analysis only account for the dominant eumelanin. Both the epidermal absorption coefficient and thickness are important in the prediction of the fluence rate loss. The computer model was used to evaluate the effect of the epidermal absorption coefficient (a parameter dictated by an individual’s skin phototype) and the epidermal thickness on the fluence rate loss through the skin. The epidermal absorption is strongly wavelength dependent with the higher absorption at the shorter wavelengths. In the computer model a longer wavelength of 676 nm (typical for a photodynamic treatment (PDT) of cancer) was used. For the darker skin phototypes (V) only about 30% of the initial laser fluence rate reached a depth of 200 ìm into the skin (just into the dermis). For the PDT application, results from the computer model indicated that treatment times need to be increased by as much as 50% for very dark skin phototypes when compared to that of very light phototypes.Thesis (PhD)--University of Pretoria, 2012.
Physics
unrestricted
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Miller, Melina Rose, and Melina Rose Miller. "Best practice guidelines for skin-to-skin contact following birth." Thesis, The University of Arizona, 2017. http://hdl.handle.net/10150/626827.
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The purpose of this thesis was to develop an educational pamphlet with best practice guidelines for expectant parents and nurses about skin-to-skin contact. The research conducted for this thesis focused on the benefits of skin-to-skin contact for mothers, pre-term infants, and term infants following both vaginal and cesarean deliveries. Skin-to-skin contact has been referred to as the optimal form of care for a newborn (Erlandsson, Dsilna, Fagerberg, & Christensson, 2007). When skin-to-skin contact does not occur following birth, the most common reasons are lack of education among parents and lack of collaboration of the healthcare team (Zwedberg, Blomquist, & Sigestad, 2013). Some of the benefits skin-to-skin contact has to offer for mothers is as a reduction in anxiety, depression, and postpartum hemorrhage (Moore, Anderson, & Bergmen, 2009). Some of the benefits of skin-to-skin contact for infants is a reduction in sepsis, infection, and hypothermia. Pre-term infants are also more likely to breastfeed and gain more weight daily if they engage in skin-to-skin contact (Conde-Agudelo & Díaz-Rossello, 2016). Implementing an educational pamphlet within the setting of a childbirth class would provide expecting parents with evidence-based information on the benefits and feasibility of skin-to-skin contact.
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Harlow, Brittany E. "Changes to the Equine Hindgut Microflora in Response to Antibiotic Challenge." UKnowledge, 2012. http://uknowledge.uky.edu/animalsci_etds/12.
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Antibiotics are important to equine medicine, but can cause detrimental side-effects including reduced feed intake, allergic reactions, and diarrhea. Antibiotic-associated diarrhea (AAD) is attributed to disruption of the hindgut microflora, permitting proliferation of pathogenic microbes. The objectives were to evaluate the effects of antibiotics on beneficial fecal bacteria, AAD-associated pathogens, microbial species richness and fermentation. Horses were assigned to treatment groups: control (no antibiotics, n=6), trimethoprim-sulfadiazine (oral, n=6), or sodium ceftiofur (IM, n=6). Fecal samples were taken during adaptation (3 wk), antibiotic challenge (1 wk), and withdrawal (1 wk). Fecal cellulolytics decreased by >99% during challenge and did not recover during withdrawal (P < 0.0001). Lactobacilli decreased by >60% during challenge (P = 0.0453). Salmonella spp. increased 94% with trimethoprim-sulfadiazine challenge (P = 0.0115). There was no detectable Clostridium difficile during adaptation or in any control horse. C. difficile increased (P < 0.0001) when horses were challenged, and remained elevated 7 d after withdrawal. There was no effect of challenge on in vitro digestibility or microbial species richness as evaluated by denaturing gradient gel electrophoresis (P > 0.05). These results indicate that antibiotics can disrupt the normal flora and allow proliferation of pathogens, even without affecting digestibility and causing AAD.
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John, Jenny, Kathrin Roediger, Wieland Schroedl, Nada Aldaher, and Ingrid Vervuert. "Development of intestinal microflora and occurrence of diarrhoea in sucking foals." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-160958.
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Background: Almost all foals develop transient diarrhoea within the first weeks of life. Studies indicated different viral, bacterial, and parasitic causes, such as rotavirus, Clostridium perfringens, Escherichia coli, and Cryptosporidium are discussed. But little is known about the development of intestinal microflora in foals. The present study investigated whether the supplementation with Bacillus cereus var. toyoi would modify the developing intestinal microflora and consequently reduce diarrhoea in foals. From birth, the foals were randomly assigned to three treatment groups: placebo (10 mL isotonic NaCl, n = 8), low dosage (LD; 5 × 108 cfu B. cereus var. toyoi, n = 7) and high dosage (HD; 2 × 109 cfu B. cereus var. toyoi, n = 10). Treatment groups were supplemented orally once a day for 58 days. Faeces scoring and sampling were performed within the first 24 h after birth and on day 9, 16, 23, 30, 44, 58 of the foal’s life and also on the first day of diarrhoea. Culture-plate methods were used to analyse the bacterial microflora. Results: Eighty-eight per cent of the foals developed diarrhoea (placebo 7/8, LD 5/7, HD 10/10)
during the first 58 days of life. Bacillus cereus var. toyoi supplementation had no effect on bacterial microflora. Clostridium perfringens and enterobacteria were equally prevalent in foals with diarrhoea and those who were not afflicted. Conclusions: We conclude that the supplementation of B. cereus var. toyoi had no effect on the occurrence of diarrhoea and health status in the foals.
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Allen, Rachel Louise. "Effect of a competitive microflora on Salmonella recovery from freeze-thawing." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287186.
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Darby, Ivan B. "Changes in the microflora and humoral immune response following periodontal therapy." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312134.
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Hoey, Leane. "Influence of soya-milk feeding on the human infant gut microflora." Thesis, University of Ulster, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273788.
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Kenche, Harshavardhan. "Validation Of A Custom-made Microarray To Study Human Intestinal Microflora." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1222096282.
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Chen, Lei. "The impact of herbal saponins on gut microflora in animal models." HKBU Institutional Repository, 2014. https://repository.hkbu.edu.hk/etd_oa/55.
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Human gut harbors 100 trillion microbial organisms that is intrinsically linked to individual’s health and diseases, including cancer. Food fiber and phytochemicals such as polyphenols are considered as prebiotic-like dietary modifiers. They can influence the gut microbial communities, and in turn to modulate disease outcome and drug responses of the host. Saponins belong to a family of phytochemicals commonly found in many medicinal and edible plants. Herbal saponins have raised keen interest among scientists for their health-promoting effects, but have not been investigated for their potential as prebiotics. Gynostemma pentaphyllum (Gp) is riched in triterpenoid saponins and has been consumed in China and other part of the world as an herbal tea and as a folk medicine. In our lab, we have demonstrated that Gp possesses strong anticancer and anti-inflammatory effects. Whether Gp possesses prebiotic property and whether gut microbiota plays any part of the anticancer effect of Gp are the questions addressed in the present study. Thus, we hypothesized that Gp saponins (GpS) might modulate the gut microbiota, which in turn enhance its anticancer activities. In the study, the gut microbiome analysis were carried out using two main techniques, neamly the enterobacterial repetitive intergenic consensus (ERIC-PCR) and 16S pyrosequencing approaches. Both xenograft nude mice and Apcmin/+ mice were employed as the animal models to investigate the interaction between the herbal saponins and the gut microbiota in the host. Athymic nude mice have been employed for tumorigenic research for decades, however, the relationships between the gut microbiome and host’s response to the grafted tumors and drug treatments are unexplored. For the first part of the thesis, we investigated the relationship between the gut microbiota and grafted tumor in the nude mice under the treatment of Gp saponins. Partial least squared discriminant analysis (PLS-DA) of ERIC-PCR data showed that the microbiota profile of xenograft nude mice departed from that of the nonxenograft mice. However, prolonged treatment of GpS seems to realign the fecal microbiota with the pretreatment control. Pyrosequencing data reiterated the differences in fecal microbiome between the nonxenograft and xenograft animals. GpS treatment had a much stronger impact on the phylotypes of the xenograft than the nonxenograft mice. In addition, GpS treatment markedly induced the relative abundance of Clostridium cocleatum and Bacteroides acidifaciens, for which the beneficial effects on the host have been well documented. ApcMin/+ colorectal cancer mouse model was further employed for the investigation of the association of the gut microbiota and cancer occurred inside the gut, which was a more direct site to interact with the gut microbiota. In the ApcMin/+ mouse model, we found distinct difference of fecal microbiome between the ApcMin/+ and the wild-type littermates. GpS treatment significantly reduced the number of intestinal polyps. GpS also increased the ratio of Bacteroidetes/Firmicutes and reduced the sulfate- and sulfur-reducing bacteria lineage and potential opportunistic pathogens, which might cause certain deleterious effects to the host. The impact of GpS on the gut mucosal environment was also examined. We found GpS treatment improved the gut barrier function by increasing the numbers of Paneth cells, goblet cells, up-regulating the expression of E-cadherin and down-regulating the expression of N-cadherin in the intestine. In addition, GpS treatment down-regulated the protein expression of beta-catenin and p-STAT3. Furthermore, higher levels of anti-inflammatory and tissue repair-related cytokines as well as Arginase I, but lower level of iNOS expression were found in GpS-treated ApcMin/+ mice, indicating increased anti-inflammatory macrophage phenotype M2 (associated with tissue repair) and reduced proinflammatory phenotype M1. Furthermore, in addition to GpS, other herbal saponins also showed prebiotic-like effects in C57BL/6 mice. In summary, this study provides first hand evidence for the impact of herbal saponins on the gut microbial ecosystem and new insight into mechanisms responsible, at least in part, for the activities of GpS. We demonstrate that tumor growth induce intestinal dysbiosis. GpS treatment can inhibit tumor progression and concurrently alter the microbiome by increasing symbionts and/or decreasing pathobionts, which may contribute to its chemopreventive effect against tumorigenesis. Herbal saponins showing prebiotic-like effects may be used for improving the health of the host by manipulation of the gut microbiota.
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Eriksson, Sofia. "Beyond the skin." Thesis, Konstfack, Ädellab, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-6409.
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This paper presents an investigation in stages of transformation between body and ornament to show upon their fluid relationship and their role for the creation of human identity. I see the act of adorning the body as an act of desire, in which we take part to become something more elevated than our original state of being. I use my background as a jeweller and the body as the territory for investigating these acts of transformation. Through material explorations I examine how we mirror ourselves in the things we make and how we reinvent our bodies through making. I compare the role of the maker with the scientist and discuss the increased interest in the body and its relation to a more and more virtual reality. I make links between ancient body modifications and human enhancement within new technology and throughout the paper I discuss my work in relation to other artists work and theorists relating to visual practices.
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McCaffrey, Clare Alexis. "Inhabiting the Skin." Thesis, Virginia Tech, 2006. http://hdl.handle.net/10919/35220.
Full textAbstract:
Appreciation of the natural light, air, and scenery outside the built interior environment spurs the architect's desire to "bring the outside in". (Lately, floor-to-ceiling glazing is the most popular way of doing this.) Appreciation of what can be found "in the wall" also inspires the architect. Neither inside nor outside, being "in the wall" offers unique sensory experiences. Historically, the thick masonry walls of public buildings provided spaces (such as the arched vestibule of a library) within the walls' openings where people could gather to talk or wait out the rain. Another example of space within walls is the window seat, which is the architect's response to many peoples' desire to curl up inside a window to read a book or to watch what's going on outside. Modern materials and technologies have allowed us to build buildings taller and lighter than ever before but they also have led us to cease creating those special spaces, neither inside nor outside, that protect people from the elements without entirely cutting them off from them.
It is this third space, neither inside nor outside, that I seek to reclaim in this thesis project. I hope to return to people space of fresh air, indirect, natural light, sense of protection, and openness; a place where stories are traded, secrets are whispered, and stars are wished on. With this project, I seek to develop a third space.Master of Architecture
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Malhi, Jaspal Singh. "Some factors affecting the microbial status of the mammalian uterus." Thesis, University of Brighton, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293299.
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Sharma, R. "Modulation of carbohydrate expression in intestinal mucosa." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285783.
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