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Journal articles on the topic 'Micronucleos test'

Author: Grafiati

Published: 4 June 2021

Last updated: 12 February 2022

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1

Wojcik, A., M. Kowalska, E. Bouzyk, et al. "Validation of the micronucleus-centromere assay for biological dosimetry." Genetics and Molecular Biology 23, no. 4 (2000): 1083–85. http://dx.doi.org/10.1590/s1415-47572000000400055.

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The micronucleus assay is frequently used for purposes of biological dosimetry. Due to high interindividual variability in the spontaneous frequency of micronuclei, its sensitivity in the low dose region is poor. It has been suggested that this problem can be mitigated by selectively analyzing the frequency of those micronuclei which contain only acentric fragments. Using a pan-centromeric FISH probe we have studied the dose dependence of micronuclei with centromeres in peripheral lymphocytes of human donors. In contrast to previous publications, our approach is based on determining the relati

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Zaydullin, Iskander I., Lilija K. Karimova, Milyausha F. Kabirova, Guzjal’ R. Sadrtdinova, Rasima R. Galimova, and Elvira T. Valeeva. "Assessment of periodontal conditions in workers exposed to harmful substances using the micronucleus test." Hygiene and sanitation 99, no. 9 (2020): 956–60. http://dx.doi.org/10.47470/0016-9900-2020-99-9-956-960.

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The purpose of this study was to identify the possibility of using a micronucleus test to assess the impact of harmful chemicals on the condition of periodontal tissues of employees of a mining and processing plant. Material and methods. Dental examination was conducted for 40 employees of the mining and processing plant, exposed to harmful substances with an experience of more than 10 years, 36 employees of the plant with a contact time of not more than 10 years, and 46 people with a healthy periodontium. The analysis of buccal epithelial cells for the presence of chromosomal aberrations usin

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3

Simakov, Yu G., S. A. Livinskaya, S. V. Smorodinskaya, and V. A. Purtskhvanidze. "Evaluation of the genome stability of schoolchildren with different food preferences by micronucleus test." Tovaroved prodovolstvennykh tovarov (Commodity specialist of food products), no. 2 (February 15, 2021): 138–42. http://dx.doi.org/10.33920/igt-01-2102-09.

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The results of questionnaire and micronucleus testing of a group of 10-11 years old students are presented. The infl uence of food preferences on stabilization and destabilization of the genome was assessed using the micronucleus test (MNT) on the buccal epithelium. The greatest amount of micronuclei (MN) was found in schoolchildren who prefer dairy products (15±2.8) and cereals (19±1.4). The destabilization of the genome, determined by the MNT test, is most noted in schoolchildren who prefer dairy dishes and cereals. A value close to the average value typical for the schoolchildren population

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Michalová, V., M. Galdíková, B. Holečková, S. Koleničová, and V. Schwarzbacherová. "Micronucleus Assay in Environmental Biomonitoring." Folia Veterinaria 64, no. 2 (2020): 20–28. http://dx.doi.org/10.2478/fv-2020-0013.

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AbstractNowadays many chemicals are widely used in agriculture to ensure high crop yields or in veterinary/human medicine to cure diseases. After their improper usage they may contaminate the environment, persist in it and adversely affect both the target and/or the non-target organisms. One of the ways to detect the occurrence of chemicals in the environment is to assess their impact on aquatic and farm animals; both are directly or indirectly exposed via their feed and water. The micronucleus assay is a standardly used cytogenetic test for the simultaneous detection of clastogenic and aneuge

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Suzuki, Y., H. Shimizu, and Y. Nagae. "Micronucleus test and erythropoiesis. Effects of erythropoietin on induction of micronuclei." Mutation Research/Environmental Mutagenesis and Related Subjects 182, no. 6 (1987): 380–81. http://dx.doi.org/10.1016/0165-1161(87)90139-7.

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6

Matthopoulos, Demetrios P. "Dynamic Analysis of DNA Damage by Flow Cytometry and FISH." Scientific World JOURNAL 6 (2006): 563–70. http://dx.doi.org/10.1100/tsw.2006.112.

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The micronucleus assay, developed to assess DNA damage induced by noxious agents, supplies information on whether the damage is due to clastogenic or aneugenic action. Although it is the test that can be used to assess agents' toxicity, it cannot provide information on the molecular events that result in the induction of micronuclei. To study the molecular events, the combination of both microscopic and analytical techniques is required. Flow-sorting induced micronuclei, based on their DNA content, in combination with chromosomal FISH and other molecular techniques, may provide information on

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7

Polívková, Z., M. Langová, P. Šmerák, B. Bártová, and I. Bárta. "Antimutagenic effect of genistein." Czech Journal of Food Sciences 24, No. 3 (2011): 119–26. http://dx.doi.org/10.17221/3306-cjfs.

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A great variety of health benefits including the protection against breast and prostate cancers has been attributed to the soya consumption, because of the presence of soy beans isoflavones, genistein, and others. We investigated the antigenotoxic effect of genistein on the genotoxicity of three mutagens and carcinogens &ndash; aflatoxine B<sub>1</sub> (AFB<sub>1</sub>), 2-amino-3-methylimidazo [4,5-f]quinoline (IQ), and N-nitroso-N-methylurea (MNU), using the Ames bacterial mutagenicity test and the micronucleus test. In the Ames test on Salmonella typhimurium, a s

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Padilla-Camberos, Eduardo, Moisés Martínez-Velázquez, José Miguel Flores-Fernández, and Socorro Villanueva-Rodríguez. "Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americanaMill., c.v.Hass)." Scientific World Journal 2013 (2013): 1–4. http://dx.doi.org/10.1155/2013/245828.

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The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products.Persea americanaseeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract ofPersea americanawas evaluated with respect to its genotoxic potential through micronucleus assay in rodents. The frequency of micronuclei in groups of animals treated with avocado seed extract showed no differences compared to the negative control (vehicle); therefor

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9

Petrovic, Sandra, Andreja Leskovac, and Gordana Joksic. "Positive correlation between micronuclei and necrosis of lymphocytes in medical personnel occupationally exposed to ionizing radiation." Archive of Oncology 13, no. 2 (2005): 65–68. http://dx.doi.org/10.2298/aoo0502065p.

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BACKGROUND: Current radiation protection standards are based on premise that any radiation dose may result in detrimental health effects. The aim of this study was to evaluate extent of the DNA damages (measured by induction of micronuclei) and interphase cell death in circulating lymphocytes of medical personnel exposed to ionizing radiation. METHODS: Baseline micronuclei were assessed using the cytokinesis-block micronucleus test. Cytotoxicity was analyzed by flow cytometry for human white blood cells to identify cells that displayed apoptosis-associated DNA condensation. Necrotic cells were

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Olvya, Shintia, Isti Rahayu Suryani, and Rurie Ratna Shantiningsih. "Perbedaan peningkatan jumlah mikronukleus antara mukosa gingiva dan mukosa bukal akibat paparan radiografi panoramik digital." Jurnal Radiologi Dentomaksilofasial Indonesia 3, no. 2 (2019): 1. http://dx.doi.org/10.32793/jrdi.v3i2.483.

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Objectives: The purpose of this study was to determine the differences in the number of micronuclei between the gingival mucosa and the buccal mucosa due to exposure to digital panoramic radiography. Material and Methods: This study used 10 healthy individuals aged 18-25 years. Exfoliated epithelial cell samples from the gingival and buccal mucosa were taken from the patient on the 10th day after exposure to digital panoramic radiography. The coloring method used is Feulgen-Rossenbeck. Results: The micronucleus calculation was performed by the method of assessing 1000 cells per sli

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Nagae, Y., T. Hayashi, Y. Suzuki, and H. Shimizu. "The micronucleus test and erythropoiesis. Effects of estrogen on induction of micronuclei." Mutation Research/Environmental Mutagenesis and Related Subjects 252, no. 1 (1991): 98. http://dx.doi.org/10.1016/0165-1161(91)90290-o.

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Velickova, Nevenka, Misko Milev, Tatjana Ruskovska, Biljana Petrova, Bojana Nedeljkovik, and Pale Gorgieva. "Cytogenetic abnormalities in lymphocytes evaluated with micronucleus assay in medical personnel occupationally exposed to ionizing radiation." Genetika 47, no. 3 (2015): 927–39. http://dx.doi.org/10.2298/gensr1503927v.

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The aim of this study was to evaluate the genotoxicity of ionizing radiation on medical personnel using the micronucleus assay and to determine the human health risk. Paired Student?s t-test shows significant statistical difference between the total number of binucleated (BN) cells with micronuclei within the two groups (exposed and control) (t=6,812; p<0,05). The mean of MN frequencies in the exposed group increased in comparison with the mean of MN frequencies in the control group. The formation of small and large micronuclei indicates that medical personnel who are exposed on radiation i

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Jeličová, Marcela, Anna Lierová, Zuzana Šinkorová, and Jaroslav Pejchal. "CHANGES IN BLOOD COUNT AND LYMPHOCYTE MICRONUCLEI IN PIGLETS AFTER WHOLE-BODY IRRADIATION." Radiation Protection Dosimetry 186, no. 2-3 (2019): 176–80. http://dx.doi.org/10.1093/rpd/ncz198.

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Abstract Biodosimetry is focused on effects of ionizing radiation at cellular and molecular levels of living organisms so that a qualified retrospective estimate of radiation load can be made. Two biodosimetry methods were evaluated in irradiated piglets: complete blood count analysis and quantification of chromosomal aberrations in lymphocytes using a micronucleus test. Animals were whole-body irradiated with gamma radiation at doses of 0–10 Gy. The analysis of complete blood count was performed at intervals ranging from 0 to 48 hours. Micronuclei were measured at 4 hours after irradiation. C

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Güez, Camila Martins, Emily Pansera Waczuk, Karina Braccini Pereira, Marcus Vinícius Morini Querol, João Batista Teixeira da Rocha, and Luís Flávio Souza de Oliveira. "In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum." Brazilian Journal of Pharmaceutical Sciences 48, no. 3 (2012): 461–67. http://dx.doi.org/10.1590/s1984-82502012000300013.

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The use of plants as a source of palliative or cure for pathological conditions is quite common worldwide. Xanthium spinosum (Asteraceae), popularly known in Brazil as 'espinho de carneiro', is an annual weed from South America, which has been used by empiric medicine to treat neoplasias. Owing to the extensive use of the above-mentioned plant and to the lack of reports about the real effects of its infusion, current study evaluated the genotoxic potential of its aqueous extract at concentrations 0.02 g L-1, 0.1 g L-1 and 0.2 g L-1 by fish micronucleus test and by comet human leukocytes assay.

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15

Clode, S. A., R. A. Riley, S. D. Blowers, T. C. Marrs, and D. Anderson. "Studies on the Mutagenicity of a Zinc Oxide-Hexachloroethane Smoke." Human & Experimental Toxicology 10, no. 1 (1991): 49–57. http://dx.doi.org/10.1177/096032719101000109.

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1 A suitable method has been developed for generating atmospheres of zinc oxide/ hexachloroethane smoke (ZnHCE). 2 The smoke was investigated using the Ames test and the micronucleus assay. 3 It was weakly mutagenic to the bacteria, but in the bone marrow no increases in micronuclei were detected up to toxic levels of the smoke. 4 The method used here could be applied to other pyrotechnic mixtures which give rise to complex mixtures of products.

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Fedoseev, Vladislav I., Danil D. Stepanov, and Varvara I. Minina. "Studying the genotoxic effects of the working environment on the workers of a coal-fired power plant using a micronuclear test on blood lymphocytes." Ecological genetics 19, no. 1 (2021): 77–88. http://dx.doi.org/10.17816/ecogen42363.

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Background: The micronucleus test of peripheral blood lymphocytes provides a qualitative assessment of the state of the genome. A study was carried out aimed at studying the influence of factors of the working environment of coal-fired power plants on the state of the DNA of working data of enterprises. Materials and methods: Using a micronucleus test with a cytochalasin block on human blood lymphocytes in vitro, 116 men were examined: 45 employees of the Novo-Kemerovo coal-fired power plant and 71 healthy residents of the Kemerovo region. Results: An increase in the frequency of o

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Westphal, Götz, Soha Asgari, Thomas Schulz, Jürgen Bünger, Michael Müller, and Ernst Hallier. "Thimerosal induces micronuclei in the cytochalasin B block micronucleus test with human lymphocytes." Archives of Toxicology 77, no. 1 (2003): 50–55. http://dx.doi.org/10.1007/s00204-002-0405-z.

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El Safty, Amal Mohamed Kamal, Aisha Mohamed Samir, Mona Kamal Mekkawy, and Marwa Mohamed Fouad. "Genotoxic Effects Due to Exposure to Chromium and Nickel Among Electroplating Workers." International Journal of Toxicology 37, no. 3 (2018): 234–40. http://dx.doi.org/10.1177/1091581818764084.

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Using chromium and nickel for electroplating is important in many industries. This process induces variable adverse health effects among exposed workers. The aim of this study is to detect the genotoxic effects of combined exposure to chromium and nickel among electroplating workers. This study was conducted on 41 male workers occupationally exposed to chromium and nickel in the electroplating section of a factory compared to 41 male nonexposed individuals, where full history and clinical examination were performed. Laboratory investigations included measurement of serum chromium, nickel, 8-hy

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Vidya, Puthan Variyam, and Kumari Chidambaran Chitra. "Evaluation of Genetic Damage in Oreochromis mossambicus Exposed to Selected Nanoparticles by Using Micronucleus and Comet Bioassays." Croatian Journal of Fisheries 76, no. 3 (2018): 115–24. http://dx.doi.org/10.2478/cjf-2018-0015.

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Abstract The purpose of the present study is to extend knowledge on the adverse effects of nanoparticles by evaluating genotoxicity as environmental risk assessment in Oreochromis mossambicus. Fish were exposed to sublethal concentrations of the selected nanoparticles, namely silicon dioxide (SiO2NPs-12mg/L), aluminium oxide (Al2O3NPs-4mg/L), titanium dioxide (TiO2NPs-16.4mg/L) and iron oxide (Fe3O4NPs-15mg/L) for short-term (24, 72 and 96 h) and long-term durations (15, 30 and 60 days). Genetic damages such as cytoplasmic, nuclear and DNA damage were measured in the erythrocytes of fish by us

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Sriambika, K. "Assessment of DNA damage through micronucleus studies in subjects exposed to formalin." Medpulse International Journal of Anatomy 19, no. 1 (2021): 01–05. http://dx.doi.org/10.26611/10011911.

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Background: Formaldehyde (FA) is the reactive and simplest of all the aldehydes. It is used as a preservative in anatomy, pathology and forensic laboratories. The international agency for research on cancer has classified FA as a carcinogen that can cause nasopharyngeal carcinoma, Leukaemia, Liver and pancreatic cancer. Objective And Method: The aim of the study was to assess the DNA damage in peripheral blood lymphocytes and in buccal cells by Micronucleus assay in Formalin exposed workers of Anatomy, Pathology and Forensic laboratories and compare with the control group, and also to analyze

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Suzuki, Yuji, Hidesuke Shimizu, Yusuke Nagae, Masakatsu Fukumoto, Hideo Okonogi, and Makoto Kadokura. "Micronucleus test and erythropoiesis: Effect of cobalt on the induction of micronuclei by mutagens." Environmental and Molecular Mutagenesis 22, no. 2 (1993): 101–6. http://dx.doi.org/10.1002/em.2850220208.

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22

Manzoor, Sathick, Kalaiselvi Santhosh, Anu Sushanth, Sakthidaran Seralathan, Vivekanandan Rajasekar, and Anoop Jacob. "A Cross-sectional Study to Evaluate Nuclear Changes in Buccal Mucosa Following Panoramic Radiography." Journal of Contemporary Dental Practice 21, no. 11 (2020): 1258–61. http://dx.doi.org/10.5005/jp-journals-10024-2921.

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ABSTRACT Aim and objective To evaluate the possible genotoxic effect of X-rays on buccal mucosa while exposing to dental panoramic radiography using micronucleus test. Materials and methods The study group comprised of 30 healthy subjects, 15 males and 15 females, aged between 24 years and 65 years. Samples were obtained from the exfoliated oral mucosa cells of buccal mucosa before and 12 days after exposing the patients to panoramic radiography. Results The study reported that there was no significant increase in the number of micronuclei cells present before and after panoramic radiography.

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Slowinski, Jerzy, Grazyna Bierzynska-Macyszyn, Urszula Mazurek, et al. "CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY IN HUMAN GLIOMA CELLS EXPOSED TO RADIATION." Image Analysis & Stereology 23, no. 3 (2011): 159. http://dx.doi.org/10.5566/ias.v23.p159-165.

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Biological tests are efficient in reflecting the biological influences of several types of generally harmful exposures. The micronucleus assay is widely used in genotoxicity studies or studies on genomic damage in general. We present methodological aspects of cytokinesis-block micronucleus assay performed in human gliomas irradiated in vitro. Eight human glioblastoma cell lines obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany) were gamma-irradiated (60Co) over a dose range of 0-10 Gy. Cytokinesis-block micronucleus assay was performed to quantitate cytog

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MANDELOVÁ, L., and J. TOTUŠEK. "Chemoprotective Effects of Broccoli Juice Treated with High Pressure." Czech Journal of Food Sciences 24, No. 1 (2011): 19–25. http://dx.doi.org/10.17221/3289-cjfs.

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We investigated chemoprotective effects of broccoli juice, treated with high pressure method, using 500 MPa for a period of 10 minutes. By the use of this method, the conservation of nutritionally important substances occurs (for example vitamins, polyphenolic compounds, glucosinolates and other constituent substances). We followed the inhibition of mutagenicity of the model mutagen, N-nitroso-N-methylurea (MNU), by means of in vivo micronucleus test. After fourteen-day application of broccoli juice (0.2 ml/10 g of body weight of mouse) and after a single administration of MNU mutagen (50 mg/k

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Ferraz, Gregory Alves, Antônio de Oliveira Costa Neto, Eneida de Moraes Marcílio Cerqueira, and José Roberto Cardoso Meireles. "Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities." Revista Brasileira de Geriatria e Gerontologia 19, no. 4 (2016): 627–34. http://dx.doi.org/10.1590/1809-98232016019.150155.

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Abstract The effects of aging, gender and lifestyle factors on inducing chromosomal damage (micronuclei) and nuclear degenerative changes were assessed using the micronucleus test on exfoliated cells of the oral mucosa. The sample included 80 healthy subjects divided into four groups according to age and gender: men and women aged 19-29 years (M19, W19) and men and women aged over sixty years (M60, W60). An interview questionnaire was used to characterize the sample and to determine an index reflecting lifestyle (HLI). The frequency of micronuclei and nuclear degenerative changes was significa

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Tobólska, Sylwia, Sylwia Terpiłowska, Jerzy Jaroszewski, and Andrzej Krzysztof Siwicki. "Genotoxicity and mutagenicity of inosine pranobex." Journal of Veterinary Research 62, no. 2 (2018): 207–13. http://dx.doi.org/10.2478/jvetres-2018-0030.

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AbstractIntroductionInosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries worldwide. In humans, the drug is approved for the treatment of viral infections, and it might also have therapeutic use in animals. The aims of the presented work were to investigate the genotoxicity of inosine pranobex on BALB/3T3 clone A1 and HepG2 cell lines and to elucidate its mutagenicity using the Ames test.Material and MethodsThe BALB/3T3 clone A1 and HepG2 cells were incubated with inosine pranobex at concentrations from

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Pozharskaya, Victoria Victorovna. "Cytogenetic status of blood lymphocytes in schoolchildren living middle and high latitudes." Russian Clinical Laboratory Diagnostics 66, no. 4 (2021): 213–16. http://dx.doi.org/10.51620/0869-2084-2021-66-4-213-216.

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Intensive industrial development of the northern regions of the Russian Federation is primarily associated with the extraction and processing of natural resources, which leads to environmental pollution and makes the tasks of sanitary and hygienic monitoring urgent. Rapid methods are convenient for the rapid diagnosis of the genetic well-being of the environment, one of which is the micronucleus test in combination with the cytokinetic block cytochalasin B. mitosis. At the moment, there are scanty data on the inter-latitudinal comparison of the results of cytogenetic studies on cells of variou

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Durnova, N. A., A. R. Klantsataya, M. N. Kurchatova, A. Yu Karetnikova, and A. S. Sheremetyeva. "Cytogenetic effect of caffeine in the micronucleus test." RUDN Journal of Medicine 25, no. 2 (2021): 147–53. http://dx.doi.org/10.22363/2313-0245-2021-25-2-147-153.

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Relevance. The consumption of caffeine-containing food in the modern world must necessarily be safe for humans, including should not affect the hereditary material of the body. Objective: to determine the possible effect of caffeine at the cytogenetic level by the micronucleus method on erythrocytes. Materials and Methods. The objects for the study were non-linear mice, which were divided into 6 groups - one control group and 5 experimental groups. The first experimental group and the second in the experiment received caffeine in doses of 40 mg/kg and 100 mg/kg.The control group received salin

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Чшиев, Т. В. "The modifying impact of melaxen on the genotoxic effects of chronic cadmium intoxication in rats." Nauchno-prakticheskii zhurnal «Medicinskaia genetika», no. 9(218) (September 30, 2020): 105–6. http://dx.doi.org/10.25557/2073-7998.2020.09.105-106.

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С использованием микроядерного теста было показано протекторное влияние мелаксена (10 мг/кг) при хронической экспериментальной интоксикации кадмием (0,3 мг/кг) в крови крыс, p<0,001. Исследуемый лекарственный препарат продемонстрировал способность снижать ожидаемое число микроядер и полихроматофильных эритроцитов (ПХЭ), индуцированных кадмием при двухнедельной и месячной экспозиции. При хроническом внутрижелудочном введении мелаксена не зафиксирован рост числа микроядер и ПХЭ по сравнению с контролем, p>0,05. Using the micronucleus test, the protective effect of melaxen (10 mg / kg) was

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Boa-Alma, A. S., G. Ramalhinho, D. Dias, M. L. Mathias, P. A. Carvalho, and A. P. Alves de Matos. "Genotoxic Effect of Inhaled Ambient Particulate Matter." Microscopy and Microanalysis 18, S5 (2012): 25–26. http://dx.doi.org/10.1017/s1431927612012780.

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In recent years great importance has been given to the adverse effects of particulate matter (PM) in health, and several epidemiological studies correlating the airborne particles with pulmonary injury have been carried out. The adverse effect of short- and long-term expositions to PM can, indeed, cause a variety of effects, from minor effects on the respiratory system to serious oncogenic effects that can lead to precocious death and are considered one of the top environmental public health concerns. Some cytogenetic techniques, as micronucleus assay, allow to detect chromosome breakage and l

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Pereira da Silva, Victor Hugo, Raquel de Luna Antonio, Sabine Pompeia, and Daniel Araki Ribeiro. "Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers." Acta Cytologica 59, no. 6 (2015): 474–78. http://dx.doi.org/10.1159/000443761.

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Objective: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. Study Design: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped i

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Dominici, Luca, Barbara Cerbone, Milena Villarini, Cristina Fatigoni, and Massimo Moretti. "In Vitro Testing for Genotoxicity of Indigo Naturalis Assessed by Micronucleus Test." Natural Product Communications 5, no. 7 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500711.

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In the field of cosmetic dyes, used for coloring the hair and skin, there is a clear tendency to replace the widely used synthetic dyes by natural colorants, such as henna and mixtures of henna with indigo. The aim of this study was to estimate the genotoxicity of water and DMSO solutions of indigo naturalis (prepared from Indigofera tinctoria leaves) using the cytokinesis-blocked micronucleus (CBMN) assay in the human metabolically active HepG2 cell line. The cytotoxic effects of indigo solutions were first assessed by propidium iodide and fluorescein-diacetate simultaneous staining. For both

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Santiago-Manzano, Dayna. "Staining criteria, collection and number of cells evaluated to identify micronuclei in buccal mucosa cells of agricultural workers exposed to pesticides." Mexican Journal of Medical Research ICSA 9, no. 18 (2021): 34–38. http://dx.doi.org/10.29057/mjmr.v9i18.5717.

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Although there are several publications that refer to the basic criteria established for the micronucleus (MN) test in exfoliated buccal cells, there is still a difference in the quantity, method of obtention and staining of the cells for the evaluation of the presence of micronuclei. Objective. Identify the criteria for evaluation of MN in oral mucosa cells exposed to pesticide in investigations carried out. Material and methods. A systematic review was carried out on the internet, based on articles published in Crossref, JCR, Scopus, PubMed, Google academic, using keywords such as: micronucl

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Eck-Varanka, Bettina, Nora Kováts, Katalin Hubai, Gábor Paulovits, Árpád Ferincz, and Eszter Horváth. "Screening potential genotoxic effect of aquatic plant extracts using the mussel micronucleus test." Journal of Coastal Life Medicine 4, no. 1 (2016): 36–38. http://dx.doi.org/10.12980/jclm.4.2016j5-187.

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Franco, Teresa, Serena Trapasso, Lidia Puzzo, and Eugenia Allegra. "Electronic Cigarette: Role in the Primary Prevention of Oral Cavity Cancer." Clinical Medicine Insights: Ear, Nose and Throat 9 (January 2016): CMENT.S40364. http://dx.doi.org/10.4137/cment.s40364.

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Background Cigarette smoke has been identified as the main cause of oral cavity carcinoma. Recently, the electronic cigarette, a battery-operated device, was developed to help smokers stop their tobacco addiction. This study aimed to evaluate the safety of electronic cigarettes and to establish the possible role of such device in the primary prevention of oral cavity cancer. Subjects and Methods This study included 65 subjects who were divided into three groups (smokers, e-cigarette smokers, and nonsmokers). All subjects were submitted to cytologic examination by scraping of oral mucosa. The s

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Marone, Palma Ann, Michael Lyon, Roland Gahler, Claudia Donath, Hana Hofman-Hüther, and Simon Wood. "Genotoxicity Studies of PolyGlycopleX (PGX)." International Journal of Toxicology 28, no. 4 (2009): 318–31. http://dx.doi.org/10.1177/1091581809338955.

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PolyGlycopleX (PGX), a novel dietary fiber, produces no mutagenic effects in bacterial tester strains Salmonella typhimurium TA 98, TA 100, TA 1535, and TA 1537 and Escherichia coli WP2 uvrA at concentrations of 0.316, 1.00, 3.16, 10.0, 31.6, and 100 μg/plate. No biologically relevant increases in revertant colonies of any of the 5 strains are observed at any concentration; however, a reduction at 100 μg/plate in TA 1537 is noted. PGX, analyzed for polychromatic erythrocyte micronuclei induction in mice following a single 1×, 0.5×, and 0.2× maximum tolerable dose intraperitoneal treatment, pro

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Paz, Márcia Fernanda Correia Jardim, André Luiz Pinho Sobral, Jaqueline Nascimento Picada, et al. "Persistent Increased Frequency of Genomic Instability in Women Diagnosed with Breast Cancer: Before, during, and after Treatments." Oxidative Medicine and Cellular Longevity 2018 (June 14, 2018): 1–10. http://dx.doi.org/10.1155/2018/2846819.

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This study aimed to evaluate DNA damage in patients with breast cancer before treatment (background) and after chemotherapy (QT) and radiotherapy (RT) treatment using the Comet assay in peripheral blood and the micronucleus test in buccal cells. We also evaluated repair of DNA damage after the end of RT, as well as the response of patient’s cells before treatment with an oxidizing agent (H2O2; challenge assay). Fifty women with a mammographic diagnosis negative for cancer (control group) and 100 women with a diagnosis of breast cancer (followed up during the treatment) were involved in this st

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Erdemir, Ebru Olgun, Abdulkadir Şengün, and Mustafa Ülker. "Cytotoxicity of Mouthrinses on Epithelial Cells by Micronucleus Test." European Journal of Dentistry 01, no. 02 (2007): 080–85. http://dx.doi.org/10.1055/s-0039-1698318.

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ABSTRACTObjectives: To determine the cytotoxicity of three commercial mouthrinses Klorhex, Andorex and Tanflex on buccal epithelial cells using micronucleus (MN) test.Materials and Methods: 28 patients with aged 16-24 undergone three mouthrinses’ application were analyzed before and after one week exposure. Physiologic saline was used for the control group. The MN incidence was scored in the buccal epithelial of each participants. The difference in pre- and posttreatment after one week incidence of MN and plaque (PI) and gingival indices (GI) was compared by non-parametric statistical tests.Re

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Quintero Ojeda, Jesús Emilo, Maribel Aguilar-Medina, Vicente Olimón-Andalón, et al. "Increased Micronuclei Frequency in Oral and Lingual Epithelium of Treated Diabetes Mellitus Patients." BioMed Research International 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/4898153.

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Diabetes mellitus (DM) is a metabolic disease characterized by persistent high levels of glucose in plasma. Chronic hyperglycemia is thought to increase oxidative stress and the formation of free radicals that in turn damage cells. Thus, we decided to determine the frequency of nuclear abnormalities in epithelial cells from cheek and tongue mucosa of DM patients with type 1 (DM1, treated only with insulin) and type 2 (DM2, treated with metformin) using the buccal micronucleus cytome (BMCyt) assay. Micronuclei frequency in cheek epithelial cells was higher in both DM1 (0.75 ± 0.31, P<0.001)

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Furnus, GNA, JD Caffetti, EM García, MF Benítez, MC Pastori, and AS Fenocchio. "Baseline micronuclei and nuclear abnormalities frequencies in native fishes from the Paraná River (Argentina)." Brazilian Journal of Biology 74, no. 1 (2014): 217–21. http://dx.doi.org/10.1590/1519-6984.13712.

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This work aims to establish baseline frequencies of micronuclei (MN) and nuclear abnormalities (NA) in native fish species collected in situ from the Paraná River. For this purpose, the micronucleus test was applied in peripheral blood erythrocytes from specimens obtained from samplings collected at two localities (Posadas and Candelaria, Misiones, Argentina) during the period 2007-2010. The results were statistically analyzed using the Kruskal Wallis test. Data from nine fish species were obtained, among which Steindachnerina brevipinna(Characiformes) revealed the highest baseline frequency o

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Lulla, Dr Smruti, Dr Amita Mali, Dr AashwiinMiglani, Dr VidyaDodwad, Dr SachinMangalekar, and Dr SandeepPatil. "Comparative evaluation of genotoxic effect in subjects with chronic periodontitis with and without type 2 diabetes mellitus." RGUHS Journal of Dental Sciences 11, no. 1 (2019): 29–36. http://dx.doi.org/10.26715/rjds.11_1_7.

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Aims: The aim of the study was to compare the genotoxic effect by assessing the micronucleus frequency (MNF), as a biomarker for DNA damage, in subjects with chronic periodontitis with and without type 2 Diabetes Mellitus. Methods and Material: For the study, a total of 65 subjects were take and divided into three groups. Group A included 30 subjects with Generalised Chronic Periodontitis, Group Bincluded 30 Subjects with Chronic Periodontitis with type 2 diabetes mellitus and Group C included 5 systemically and periodontally healthy subjects. Periodontal clinical examination was carried out.

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Lisina, N., K. Romanova, L. Sycheva, and L. Rozhdestvenskiy. "Comparative Evaluation of the Anti-Radiation Efficacy of Flagellin by Survival and Micronucleus Test." Medical Radiology and radiation safety 66, no. 4 (2021): 13–17. http://dx.doi.org/10.12737/1024-6177-2021-66-4-13-17.

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Purpose: Comparative evaluation of the effectiveness of the domestic drug flagellin (development of the State Research Institute of High-Purity Biological Products, St. Petersburg) in an extended range of drug administration periods before and after irradiation, as well as evaluation of the possibility of using the micronucleus test as a biomarker of its effectiveness. Material and methods: The work was performed on male ICR CD1 mice weighing 20–22g. The radioprotective effectiveness of flagellin was evaluated by the 30-day survival rate of experimental animals in comparison with control

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Stubbs, Brianna J., Andrey I. Nikiforov, Marisa O. Rihner, et al. "Genetic Toxicity Studies of the Ketogenic Ester Bis Hexanoyl (R)-1,3-Butanediol." International Journal of Toxicology 40, no. 3 (2021): 242–49. http://dx.doi.org/10.1177/1091581821991772.

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A series of studies was conducted to assess the genetic toxicity of a novel ketone ester, bis hexanoyl (R)-1,3-butanediol (herein referred to as BH-BD), according to Organization for Economic Co-operation and Development testing guidelines under the standards of Good Laboratory Practices. In bacterial reverse mutation tests, there was no evidence of mutagenic activity in any of the Salmonella typhimurium strains tested or in Escherichia coli strain WP2 uvrA, at dose levels up to 5,000 μg/plate in the presence or absence of Aroclor 1254-induced rat liver (S9 mix) for metabolic activation. In th

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Kumar, Radhakrishnan Vinodh, Park Chung Mu, Vilwanathan Ravikumar, and Thiruvengadam Devaki. "Inhibitory Effects of Histone Deacetylase Inhibitor Depsipeptide on Benzo(a)pyrene- and Cyclophosphamide-Induced Genotoxicity in Swiss Albino Mice." International Journal of Toxicology 26, no. 1 (2007): 47–50. http://dx.doi.org/10.1080/10915810601120111.

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Depsipeptide (FK228 or FR901228) was evaluated in the mouse bone marrow micronucleus test for its possible protective effect against chromosomal damage induced by benzo(a)pyrene and cyclophosphamide. Three doses of depsipeptide (0.5, 1, and 1.5 mg/kg body weight) were given intravenously to mice for 7 consecutive days prior to administration of genotoxins under investigation. All the three doses of depsipeptide were effective in exerting a protective effect against both benzo(a)pyrene and cyclophosamide. A significant suppression (34.9% to 67.5%) in the micronuclei formation induced by benzo(a

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Sen, Asuman K., Emin Karakas, and Rahmi Bilaloglu. "Genotoxic Effect of Epirubicin in Mouse Bone Marrow in vivo." Zeitschrift für Naturforschung C 65, no. 3-4 (2010): 211–17. http://dx.doi.org/10.1515/znc-2010-3-408.

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The genotoxic effect of epirubicin, a semisynthetic anthracycline antibiotic which has been used as an anticancer drug, was investigated in vivo on bone marrow cells of Swiss albino mice using the micronucleus test. To determine the incidence of micronuclei, mice were injected intraperitoneally with the drug at single doses of 4, 6, 8, and 10 mg/kg body weight. Then, bone marrow was sampled 18, 24, 36, and 48 h after the treatment. Polychromatic and normochromatic erythrocytes were examined for the presence of micronuclei. Epirubicin significantly increased the frequency of micronucleated poly

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Alimba, Chibuisi G., Ebenezer O. Ajayi, Titilayo Hassan, Akindayo A. Sowunmi, and Adekunle A. Bakare. "Cytogenotoxicity of Abattoir Effluent in Clarias gariepinus (Burchell, 1822) Using Micronucleus Test." Chinese Journal of Biology 2015 (November 17, 2015): 1–6. http://dx.doi.org/10.1155/2015/624524.

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The cytogenotoxic potential of abattoir effluent from Bodija, Nigeria, was investigated using micronucleus test in Clarias gariepinus. Fish was exposed to five different concentrations: 0.2, 0.4, 0.8, 1.6, and 3.1% of the effluent for 7, 14, and 28 days. Tap water and 0.02 mL/L of benzene were used as negative and positive controls, respectively. Physicochemical parameters and heavy metals were analyzed in the effluent in accordance with standard methods. After exposure, blood was collected from the treated and control fish and slides were prepared for micronuclei (MN) and nuclear abnormality

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Ludewig, E., F. Koch, F. Kamprad, and R. Melzer. "The micronucleus test in pigs: induction of micronuclei in polychromatic erythrocytes by various doses of X-rays." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 249, no. 1 (1991): 1–6. http://dx.doi.org/10.1016/0027-5107(91)90127-a.

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Belmont-Díaz, Javier, Ana Paulina López-Gordillo, Eunice Molina Garduño, et al. "Micronuclei in Bone Marrow and Liver in relation to Hepatic Metabolism and Antioxidant Response due to Coexposure to Chloroform, Dichloromethane, and Toluene in the Rat Model." BioMed Research International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/425070.

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Genotoxicity in cells may occur in different ways, direct interaction, production of electrophilic metabolites, and secondary genotoxicity via oxidative stress. Chloroform, dichloromethane, and toluene are primarily metabolized in liver by CYP2E1, producing reactive electrophilic metabolites, and may also produce oxidative stress via the uncoupled CYP2E1 catalytic cycle. Additionally, GSTT1 also participates in dichloromethane activation. Despite the oxidative metabolism of these compounds and the production of oxidative adducts, their genotoxicity in the bone marrow micronucleus test is uncle

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Akca, Cetin, Ozgur Vatan, Dilek Yilmaz, Huzeyfe HURIYET, Nilüfer Cinkilic, and Tolga Cavas. "In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines." Czech Journal of Food Sciences 37, No. 1 (2019): 29–35. http://dx.doi.org/10.17221/221/2018-cjfs.

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In vitro cytotoxic and genotoxic effects of donkey milk on cancer (A549) and normal (BEAS-2B) lung cell lines were investigated. The XTT and WST-1 tests as well as clonogenic assays were used to evaluate cytotoxicity. The comet assay and micronucleus test were used as genotoxicity endpoints. Donkey milk showed lower cytotoxic effects against normal lung cell line BEAS-2B in comparison to the tumor cell line A549. Genotoxicity experiments revealed dose dependent increases in the frequencies of micronuclei and single stranded DNA breaks in A549 cells whereas no significant damage was observed in

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Matsumoto, F. E., and I. M. S. Cólus. "Micronucleus frequencies in Astyanax bimaculatus (Characidae) treated with cyclophosphamide or vinblastine sulfate." Genetics and Molecular Biology 23, no. 2 (2000): 489–92. http://dx.doi.org/10.1590/s1415-47572000000200041.

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Two known mutagenic drugs, cyclophosphamide and vinblastine sulfate, were tested using the micronucleus test in the native fish species, Astyanax bimaculatus, in order to determine which of these drugs and the doses which would be the most adequate for use as positive controls in this species. This Brazilian fish species was chosen because few toxicity studies have used native fish species and this particular species is widely consumed in various regions of Brazil. Three thousand erythrocytes per specimen were scored. Doses of 16 and 8 mg/kg body weight of cyclophosphamide and vinblastine sulf

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