Academic literature on the topic 'Microorganisms – Seeds'

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Journal articles on the topic "Microorganisms – Seeds"

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Kremer, Robert J. "Antimicrobial Activity of Velvetleaf (Abutilon theophrasti) Seeds." Weed Science 34, no. 4 (1986): 617–22. http://dx.doi.org/10.1017/s0043174500067540.

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Velvetleaf (Abutilon theophrastiMedik. # ABUTH) seeds were bioassayed on 241 microbial isolates to assess their antimicrobial activity. Seeds placed on agar plates inoculated with test microorganisms released a diffusible substance(s) that inhibited the growth of 117 of 202 (58%) bacteria and all of the fungi tested. Antimicrobial activity of the seeds appeared to be nonselective as the extent of inhibition was not related to type of microorganism nor their origin. Hard, water-impermeable seeds had greater inhibitory activity than imbibed (soft) seeds. The intensity of inhibition was affected by prior leaching of seeds with various solvents and by the stage of seed development. Chemical analysis of diffusion zones from agar plates and seed leachates revealed the presence of phenolic compounds. The presence of antimicrobial substances in velvetleaf seeds may contribute to the persistence of viable seeds in soil by inhibiting potential seed-deteriorating microorganisms.
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Kremer, Robert J. "Microorganisms Associated with Velvetleaf (Abutilon theophrasti) Seeds on the Soil Surface." Weed Science 34, no. 2 (1986): 233–36. http://dx.doi.org/10.1017/s0043174500066728.

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The relationships between microorganisms and velvetleaf (Abutilon theophrastiMedic. # ABUTH) seeds in contact with soil were studied to determine microbial effects on velvetleaf seed viability. The characteristic seed microbial association persisted on velvetleaf seeds placed on the soil surface during 32 days of incubation. The fungal association comprised ofAlternaria alternata(Fr.) Keissl.,Cladosporium cladosporioides(Fres.) de Vries,Epicoccum purpurascensEhrenb. ex Schlecht, andFusariumspp. was detected on over 50% of the seeds during incubation. Establishment of soil microorganisms on 50% of seeds occurred at only one sampling date. Such establishment was largely unsuccessful due to the effective barrier presented by seedborne microorganisms. Seedborne and soilborne microorganisms were unable to reduce viability of velvetleaf seeds in contact with soil as the total viability of all seeds tested exceeded 90% during incubation. Velvetleaf seedborne microorganisms may augment seed longevity on and in soils by acting as a barrier to potential seed decomposers originating from soil.
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Silva, Isledi William da, Patrícia Carine Hüller Goergen, Luiz Volney Mattos Viau, Sandra Beatriz Vicenci Fernandes, José Antônio Gonzalez da Silva, and Cleusa Adriane Menegassi Bianchi. "Growth Promoting Microorganisms for Treatment of Soybean Seeds." Journal of Agricultural Science 10, no. 6 (2018): 198. http://dx.doi.org/10.5539/jas.v10n6p198.

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The use of microorganisms that promote plant growth is an important tool for increasing agricultural production; besides, there is an emerging demand for decreasing utilization of chemical fertilizers and to solidify sustainable agriculture. The objective of this study was to evaluate the efficacy of growth promoting microorganisms Bradyrhizobium elkanii and Trichoderma harzianum for treatment of soybean seeds. This experiment was conducted at the Regional Institute of Rural Development (IRDeR), in Augusto Pestana, RS, Brazil. The experimental design was randomized blocks and treatments were: control, Trichoderma harzianum, Bradyrhizobium elkanii, and co-inoculated Trichoderma harzianum + Bradyrhizobium elkanii, with four replications per treatment. The sample units were five rows 5 m in length, spaced at 0.5 m, and three central lines were used to analyze the components of soybean yield. Of these, two lines were harvested 4 m, and the incidence of soil diseases in the plants was evaluated in one of the lines. Treatments means were compared using Tukey test. There was an effect of treatments: B. elkanii and treatment with co-inoculation B. elkanii and T. harzianum showed a significant response to grain yield. The chemical treatment without microorganisms with a biological effect had a higher incidence of diseases and a lower yield of grains.
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Paau, Alan S. "Formulations useful in applying beneficial microorganisms to seeds." Trends in Biotechnology 6, no. 11 (1988): 276–79. http://dx.doi.org/10.1016/0167-7799(88)90124-2.

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Đerić, Nataša, Olja Todorić, Milana Rošul, et al. "Influence of different chemical agents and storage conditions on the microbiological content of industrial hemp (Cannabis sativa L.) seeds." Food and Feed Research 47, no. 2 (2020): 159–68. http://dx.doi.org/10.5937/ffr47-29058.

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This study aimed to test different chemical agents to obtain microbiologically safe industrial hemp seeds that could be used for further food processing (with the reduced total number of microorganisms, total number of moulds and yeasts, and total number of Enterobacteriaceae). In order to obtain seeds applicable for food consumption, optimal storage temperature conditions (room temperature, refrigerator, freezer), method of seed packaging (vacuum/without vacuum), and the application of various chemical treatments (ethanol, sodium hydrogen carbonate, sodium hypochlorite) were tested on the certified industrial hemp seeds, produced in two consecutive years. Optimal storage conditions differed for different microorganisms, and the most optimal storage was at room temperature, for seeds produced in 2018, in the treatment to reduce the total number of Enterobacteriaceae and the total number of microorganisms. When storing seeds from 2018 in order to reduce the number of yeasts and moulds, a slightly lower number was spotted when seeds were stored in a vacuum-sealed bag, at the refrigerator/freezer temperature. For hemp seeds produced in 2019, the most optimal storage conditions were at the refrigerator (for reduction of the total number of Enterobacteriaceae) and freezer temperature (for reduction of the total number of microorganisms). For the reduction of the total number of moulds and yeasts, optimal conditions were at room temperature. Ethanol (75%, v/v) was the most effective disinfectant among the tested chemicals regardless of the initial number of microorganisms, with log reduction of 3.2 (for the total number of Enterobacteriaceae), 2.9 log (for the total number of microorganisms), and total reduction of the total number of yeasts and moulds after 10 minutes, for the seeds harvested in 2019, which were far more contaminated than the seeds harvested in 2018. Considering the price of the disinfection method with ethanol, sodium hypochlorite may be a better solution for the reduction of the number of microbiota on the seeds.
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Wright, B., H. R. Rowse, and J. M. Whipps. "Application of Beneficial Microorganisms to Seeds during Drum Priming." Biocontrol Science and Technology 13, no. 6 (2003): 599–614. http://dx.doi.org/10.1080/09583150310001517992.

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Kadlec, P., J. Dostalova, J. Culkova, M. Houska, and J. Strohalm. "Microorganisms baroinactivation of germinated mung bean (green gram) seeds." High Pressure Research 27, no. 1 (2007): 133–38. http://dx.doi.org/10.1080/08957950601089719.

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Marroni, Igor Villela, Andrea Bittencourt Moura, and Bernardo Ueno. "Chemical and biological treatments of castor bean seeds: effects on germination, emergence and associated microorganisms." Revista Brasileira de Sementes 34, no. 1 (2012): 21–28. http://dx.doi.org/10.1590/s0101-31222012000100003.

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The effect of chemical and biological treatments on castor bean emergence, seedling vigor, dry matter production, and also the control of microorganisms associated with seeds of the AL Guarany 2002 and Lyra cultivars, was evaluated. The products tested were carbendazim + thiram, carboxin + thiram and a product based on Trichoderma. Total seed and seedling emergence were evaluated at 27 days after sowing whereas dry matter production was verified for plants removed 45 days after sowing. The Guarany 2002 AL cultivar had a higher incidence of microorganisms than the Lyra cultivar. The chemical treatment was 100% effective in controlling fungi but the biological treatment did not reduce microorganism incidence on the seeds. Chemical treatment resulted in plants with more dry matter and the best results were for carbendazim + thiram and carboxin + thiram at doses of 60 g + 140 g and 50 g + 50 g/100 kg of seeds, respectively. The carbendazim + thiram mixture was the only treatment which was statistically higher for total emergence whereas the biological treatment increased emergence only for the Lyra cultivar, thus demonstrating its lower efficiency. The importance of fungicides to control pathogens associated with seeds was discussed.
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Munkager, Victoria, Mette Vestergård, Anders Priemé, et al. "AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research." Plants 9, no. 3 (2020): 372. http://dx.doi.org/10.3390/plants9030372.

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To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant–microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H2O2, NaClO, and AgNO3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO3 by testing several concentrations of AgNO3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO3, and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO3 can be used successfully for experiments on plant–microbiome interactions.
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Martínez, Patricio, and Pilar Parada. "BioSigma Bioleaching Seeds (BBS): A New Technology for Managing Bioleaching Microorganisms." Advanced Materials Research 825 (October 2013): 305–8. http://dx.doi.org/10.4028/www.scientific.net/amr.825.305.

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In Bioleaching, although it is already prove that chalcopyrite can be dissolved by microorganisms, a major task is to do it efficiently in economical terms at industrial scale. BioSigma Bioleaching Seeds (BBS) represents a biotechnological breakthrough for the production of bioleaching solutions on demand with high concentrations of biomining microorganisms. This innovation is mainly a product based on the encapsulation of BioSigma bioleaching microorganisms in a natural matrix of alginate. This technology gives the following operational advantages: 1. High concentration of inoculum. 2. Long period of inoculum storage (more than 1 year). 3. Reduction of volume and costs of transport of bioleaching solutions. 4. Homogeneous mineral inoculation; uniform inoculation of the ore using solid capsules. 5. Protection against toxic elements to retain the viability and activity of the bioleaching solutions. 6. Addition of additives for incorporation of nutrients or other molecules that enhance the activity. 7. Encapsulation of different bioleaching microorganisms producing specific "bioleaching seeds" for each biohydrometallurgical process. All the above advantages make this new technology a very attractive alternative to enhance bioleaching processes at on site operations and overcome stressful conditions for biomining microorganisms.
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Dissertations / Theses on the topic "Microorganisms – Seeds"

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Parisi, João José Dias. "Associação entre fungos e a viabilidade de sementes de Inga vera subsp. affinis (DC.) T.D. Penn. durante o armazenamento." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256849.

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Orientadores: João Domingos Biagi, Claudio José Barbedo<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Agrícola<br>Made available in DSpace on 2018-08-19T22:55:33Z (GMT). No. of bitstreams: 1 Parisi_JoaoJoseDias_D.pdf: 700508 bytes, checksum: 9956b2ce3f085d143589b761f27c7d01 (MD5) Previous issue date: 2012<br>Resumo: O elevado metabolismo dificulta a manutenção da viabilidade de sementes de Inga vera. As principais estratégias para a conservação dessas sementes durante o armazenamento consistem na desidratação parcial, na adição de ácido abscísico, no uso de solução de polietileno glicol e de baixas temperaturas. Contudo, tais condições ainda podem conduzir à deterioração, tanto pela atividade do seu próprio metabolismo, como pelo desenvolvimento de micro-organismos favorecidos pela umidade. Desta forma, o presente trabalho teve como objetivo analisar o grau de interferência dos fungos no metabolismo respiratório dos embriões de Inga vera com diferentes níveis de hidratação e a eficiência do tratamento com fungicidas, visando à manutenção da viabilidade durante o armazenamento. Para tanto, os embriões foram obtidos em três anos, sendo em 2009 colhidos em duas fases distintas de maturação (imaturos e maduros), submetidos a dois níveis de secagem, tratados ou não com carboxin + thiram e armazenados sob 7 e 25 oC por até 65 dias. Em 2010, foram colhidos maduros, submetidos a quatro níveis de secagem, tratados ou não com carbendazin + thiram e armazenados a 7 ºC por 120 dias. Nos experimentos de avaliação da respiração, os embriões obtidos em 2010 foram tratados ou não com carbendazin + thiram, mortos ou não por congelamento e inoculados ou não com Aspergillus flavus. Os colhidos em 2011 foram mantidos sem secagem e tratados ou não com carbendazin + thiram e a respiração foi avaliada a 7 e a 25 ºC. Constatou-se que a incidência de fungos, principalmente Fusarium oxysporum, juntamente com o teor de água e a temperatura influenciam nas taxas respiratórias dos embriões de I. vera maduros e que o tratamento com carbendazin + thiram, na dose de 80 mL do produto comercial para 100 kg de embriões, é eficiente na manutenção da viabilidade de embriões maduros e sem secagem, a 7 ºC por 120 dias. Pestalotiopis sp., Phoma sp., Phomopsis diachenii e Colletotrichum gloeosporioides aparecem durante o armazenamento, simultaneamente à deterioração dos embriões de I. vera. O desenvolvimento de fungos em embriões de I. vera difere do padrão clássico definido com base em sementes ortodoxas<br>Abstract: The high metabolism hinders the maintenance of Inga vera seeds viability. The main strategies for the conservation of these seeds during storage consist of the partial dehydration, addition of abscisic acid, use of polietileno glicol solution and of low temperatures. However, such storage conditions can lead to further deterioration of the seed, either by the activity of its own metabolism, as the development of microorganisms favored by moisture. This study aimed to analyze the degree of interference of fungi on the respiratory metabolism of Inga vera embryos with different levels of hydration and the efficiency of fungicide treatment, as maintaining these embryos viability during storage. The embryos were obtained in three years. In 2009 they were collected in two distinct stages of maturation (immature and mature), subjected to two drying levels, treated or not treated with carboxin + thiram and stored at 7 and 25 º C for up to 65 days. In 2010 the embryos were collected mature, subjected to four drying levels, treated or not treated with carbendazin + thiram and stored at 7 º C for 120 days. In the experiments carried out to evaluate the respiration, the embryos obtained in 2010, were treated or not treated with carbendazin + thiram, killed or not by freezing and inoculated or not with Aspergillus flavus. The embryos collected in 2011 were kept without drying, treated or not treated with carbendazin + thiram and respiration was evaluated at 7 and 25 ° C. It was found that the incidence of fungi, primarily Fusarium oxysporum, together with the water content and temperature influence respiration rates of mature embryos of I. vera and that the treatment with carbendazin + thiram at the dose of 80 mL of the commercial product to 100 kg of embryos is effective for maintaining the viability of mature embryos without drying at 7 ° C for 120 days. Pestalotiopis sp., Phoma sp., Phomopsis diachenii and Colletotrichum gloeosporioides are detected during the storage time, along with the deterioration of the embryos of I. vera. The development of fungi on embryos of I. vera differs from the classic pattern verified for orthodox seeds<br>Doutorado<br>Tecnologia Pós-Colheita<br>Doutor em Engenharia Agrícola
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Penduka, Dambudzo. "In-vitro anti-vibrio activities of crude extracts of Garcinia Kola seeds." Thesis, University of Fort Hare, 2011. http://hdl.handle.net/10353/405.

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The n-Hexane, dichloromethane, methanol and aqueous crude extracts of Garcinia kola (Heckel) seeds were screened for their anti-Vibrio activities against 50 Vibrio bacteria isolated from wastewater final effluents. The 50 isolates consisted of different Vibrio species namely V. fluvialis (14), V. vulnificus (12), V. parahaemolyticus (12), V. metschnikovii (3) and 9 others unidentified to the specie level. The n-Hexane, dichloromethane and methanol extracts had activities against 16 (32 percent) of the Vibrio isolates, while the aqueous extracts had activities against 12 (24 percent) all at a screening concentration of 10 mg/ml. The minimum inhibitory concentrations (MICs) were 0.313-0.625 mg/ml, 0.313-0.625 mg/ml, 0.313-2.5 mg/ml and 10 mg/ml for n-Hexane, dichloromethane, methanol and aqueous extracts respectively. Rate of kill studies were carried out against three different Vibrio species namely V. vulnificus (AL042), V. parahaemolyticus (AL049) and V. fluvialis ( AL040) using the n-Hexane, dichloromethane and methanol extracts at 1× to 4 × MICs and 2 hour exposure. About 96.3 percent, 82.2 percent, and 78.1 percent (V. fluvialis AL040); 92.6 percent, 87.8 percent and 68.9 percent (V. parahaemolyticus AL049); and 91.6 percent, 64.4 percent, 60 percent (V. vulnificus AL042) of the bacteria were killed by the crude n-Hexane, dichloromethane and methanol extracts respectively after 2 hour exposure time at 4× MIC. The patterns of activity were bacteriostatic, with the n-Hexane extracts being most effective in activity. We conclude that the Garcinia kola seeds have promise in the treatment and management of infections caused by Vibrio species.
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Seanego, Christinah Tshephisho. "Phytochemical analysis and bioactivity of Garcinia Kola (Heckel) seeds on selected bacterial pathogens." Thesis, University of Fort Hare, 2012. http://hdl.handle.net/10353/420.

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Garcinia kola is one of the plants used in folklore remedies for the treatment of microbial infections. Bacterial resistance to commonly used antibiotics has necessitated the search for newer and alternative compounds for the treatment of drug resistant microbial infections. This study focuses on the bioactivity of G. kola seeds on Streptococcus pyogenes (ATCC 49399), Staphylococcus aureus (NCTC 6571), Plesiomonas Shigelloides (ATCC 51903) and Salmonella typhimurium (ATCC 13311), organisms which can cause illnesses from mild to severe with potentially fatal outcomes. The crude ethyl acetate, ethanol, methanol, acetone and aqueous extracts were screened by agar-well diffusion method and the activities of the extract were further determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays. The inhibition zones ranged from 0 - 24 mm, while MIC and MBC of the extract ranged between 0.04 - 1.25 mg/mL and 0.081 - 2.5 mg/mL respectively. Chloroform/ Ethyl Acetate/ Formic acid (CEF) solvent system separated more active compounds followed by Ethyl Acetate/ Methanol/ Water (EMW) and Benzene/ Ethanol/ Ammonium Hydroxide (BEA). The extracts were fractionated by Thin Layer Chromatography (TLC). Bioautography was used to assess the activity of the possible classes of compounds present in the more active extracts. Column chromatography was used to purify the active compounds from the mixture while Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify the phyto components of the fractions. The MIC of the fractions ranged between 0.0006 - 2.5 mg/mL. CEF 3 (F3), CEF 11 (F11) and CEF 12 (F12) revealed the presence of high levels fatty acids Linoleic acid, 1, 2-Benzenedicarboxylic acid and 2, 3-Dihydro-3, 5-dihydroxy-6-methyl, respectively. The results obtained from this study justify the use of this plant in traditional medicine and provide leads which could be further exploited for the development of new and potent antimicrobials.
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Assumpção, Laura de Castro. "Diversidade da comunidade bacteriana endofítica de sementes de soja e o seu potencial biotecnológico." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-10022009-154643/.

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Tecidos vegetais, incluindo as sementes, são habitados por microrganismos denominados endofíticos, cuja interação com a planta pode conferir características vantajosas ao hospedeiro. Sabe-se que o crescimento de plantas é influenciado por fatores como a síntese de ácido-indolacético (AIA), solubilização de fosfato, fixação de nitrogênio e controle de fungos fitopatogênicos. Antes da comercialização, as condições de armazenamento de sementes de soja podem restringir o desenvolvimento de microrganismos devido à baixa temperatura e umidade. Esse fato leva ao interesse de exploração de microrganismos endofíticos resistentes a essas condições. O estudo e a caracterização dessas comunidades são de grande interesse agronômico e biotecnológico, sendo possível sua aplicação em sementes, introduzindo no campo plantas com superior potencial de produção. Com os objetivos de comparar a comunidade bacteriana endofítica de sementes de soja geneticamente modificadas e convencionais; e de isolar e caracterizar essas comunidades, sementes de 12 cultivares de soja foram amostradas, de onde 3504 isolados bacterianos foram obtidos. Os isolados foram agrupados morfologicamente de acordo com a coloração e taxa de crescimento das colônias, sendo representantes de cada grupo morfológico (no total 176 isolados) agrupados pela técnica de ARDRA (Análise de Restrição de DNA Ribossomal Amplificado). Um total de 12 ribotipos foi observado compondo a comunidade cultivada de bactérias endofíticas de sementes de soja. Representantes destes ribotipos tiveram seus genes 16S rDNA parcialmente sequenciados, identificando os integrantes desta comunidade como: Acinetobacter sp., Bacillus sp., Brevibacterium sp., Chryseobacterium sp., Citrobacter sp., Curtobacterium sp., Enterobacter sp., Methylobacterium sp., Microbacterium sp., Micromonospora sp., Pantoea sp., Paenibacillus sp., Pseudomonas sp., Ochrobactrum sp., Streptomyces sp. e Tsukamurella sp. A comunidade bacteriana endofítica de sementes de soja provenientes de plantas genticamente modificadas apresentou uma diversidade maior comparada à comunidade bacteriana de plantas convencionais. Em relação ao potencial biotecnológico desta comunidade, os resultados demonstraram que os isolados foram capazes de controlar o crescimento de fungos fitopatogênicos por antagonismo (18%), sintetizar AIA (100%), solubilizar fosfato (39%) e fixar nitrogênio (18%). Os isolados com os melhores resultados nas análises in vitro foram inoculados em sementes de soja e avaliados em casa de vegetação quanto à habilidade de promover o crescimento das plantas. As plantas apresentaram diferentes respostas à inoculação das bactérias. A maior parte dos tratamentos mostrou influência negativa das bactéria nas plantas, enquanto que um isolado de Enterobacter sp. aumentou a massa da matéria seca de raiz. Mesmo não diferindo estatisticamente, alguns isolados mostraram tendência de aumento e outros de diminuição de biomassa da planta.<br>Plant tissues, including seeds, are inhabited by microorganisms called endophytes, whose interaction with the plant can offer advantages to the host. It is known that plants growth promotion is induced by indole acetic acid (IAA) synthesis, phosphate solubilization and nitrogen fixation, among others. The control of phytopathogenic fungi is also related to a good plant development. Before commercialization, the seed storage conditions can restrict the development of microorganism, due to the low temperature and humidity. This fact leads to the interest of exploring resistant microorganisms to those conditions. The study and the characterization of these communities are of great agronomic and biotechnological interest, being possible its application onto seeds, introducing in the field plants with a greater production potential. In this context, the aim of this study was to isolate and identify the endophytic bacteria community in soybean seeds and study the capacity of these isolates to promote growth in the host plant, including: phosphate solubilization, nitrogen fixation, IAA synthesize and antagonism against phytopathogenic fungi. From seeds of 12 cultivars, 3504 bacteria were isolated. The isolates were morphologically grouped according to the coloration and growth rate of the colony. Representatives of each morph group, totalizing 176, were analyzed using the Amplified Ribosomal Restriction Analysis (ARDRA) technique. A total of 12 ARDRA ribotypes were observed in the cultivable endophytic community of soybean seeds. Representatives of each ribotype had their 16S rDNA gene partially sequenced, allowing to the identification of the members of this community as: Acinetobacter sp., Bacillus sp., Brevibacterium sp., Chryseobacterium sp., Citrobacter sp., Curtobacterium sp., Enterobacter sp., Methylobacterium sp., Microbacterium sp., Micromonospora sp., Pantoea sp., Paenibacillus sp., Pseudomonas sp., Ochrobactrum sp., Streptomyces sp. and Tsukamurella sp. The endophytic bacterial community of soybean seeds from genetically modified plants showed a greater diversity compared to the bacterial community of conventional seeds. In relation to the biotechnological potential of the community, the outcomes demonstrate that the isolates were able to antagonist phytopathogenic fungi (18%), synthesize IAA (100%), solubilize phosphate (39%) and fix nitrogen (18%). The isolates with best in vitro outcomes were inoculated onto seeds and tested in greenhouse for their ability to promote growth in soybean. The plants answered differently to the inoculation of each bacterial isolate. The major part of the treatments demonstrated a negative influence of bacteria onto plants, while one Enterobacter sp. isolate increased the dry mass weight of roots. Even not differing statistically, some isolates showed a tendency to increase, meanwhile others to decrease the biomass of the plant.
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Sibanda, Thulani. "Assessment of antibacterial potentials of Garcinia Kola seed extracts and their interactions with antibiotics." Thesis, University of Fort Hare, 2007. http://hdl.handle.net/10353/71.

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The antibacterial potency of the extracts of the seed of Garcinia kola (bitter kola) was investigated in this study against a panel of referenced, environmental and clinical bacterial strains. The killing rates of the active extract as well as their potential for combination antibacterial therapy with standard antibiotics were also elucidated using standard procedures. The aqueous and acetone extracts of the seed were screened for activity against 27 bacterial isolates. The aqueous extract exhibited activity mainly against Gram positive organisms with Minimum inhibitory concentration (MIC) values ranging from 5 mgml-1 – 20 mgml-1, while the acetone extract showed activity against both Gram negative and Gram positive organisms with MIC values ranging from 10 mgml-1 - 0.156 mgml-1. The acetone extract also showed rapid bactericidal activity against Staphylococcus aureus ATCC 6538 with a 3.097 Log10 reduction in counts within 4 hours at 0.3125 mgml-1 and a 1.582 Log10 reduction against Proteus vulgaris CSIR 0030 at 5 mgml-1 after 1 hour. In addition, the aqueous, methanol and acetone extracts of the seeds also exhibited activity against four clinical strains of Staphylococcus isolated from wound sepsis specimens. The MIC values for the aqueous extract were 10 mgml-1 for all the isolates while the acetone and methanol extracts had lower values ranging from 0.3125 - 0.625 mgml-1. The acetone extract was strongly bactericidal against Staphylococcus aureus OKOH3 resulting in a 2.70 Log10 reduction in counts at 1.25 mgml-1 within 4 hours of exposure and a complete elimination of the organism after 8 hours. The bactericidal vi activity of the same extract against Staphylococcus aureus OKOH1 was weak, achieving only a 2.92 Log10 reduction in counts at 1.25 mgml-1 (4× MIC) in 24 hours. In the test for interactions between the acetone extract of the seeds and antibiotics, synergistic interactions were observed largely against Gram positive organisms using the FIC indices, (indices of 0.52 - 0.875) with combinations against Gram negatives yielding largely antagonistic interactions (indices of 2.0 to 5.0). Synergy (≥ 1000 times or ≥ 3 Log10 potentiation of the bactericidal activity) against both Gram negative and Gram positive organisms was detected by time kill assays mainly involving the antibiotics tetracycline, chloramphenicol, amoxycillin and penicillin G. Combinations involving erythromycin and ciprofloxacin consistently gave antagonistic or indifferent interactions. We conclude that the acetone extract of Garcinia kola seeds possess strong bactericidal activities against both Gram positive and Gram negative organisms and can be therapeutically useful in the treatment of bacterial infections including the problematic staphylococcal wound infections. In addition, the acetone extract can be a potential source of broad spectrum resistance modifying compounds that can potentially improve the performance of antibiotics in the treatment of drug resistant infections.
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Cummings, Jaime Anne. "Evaluation of seed and drench treatments for management of damping-off and seedling blight pathogens of spinach for organic production." Online access for everyone, 2007. http://www.dissertations.wsu.edu/Thesis/Fall2007/j_cummings_112007.pdf.

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Rezki, Samir. "Structuration, dynamique et réponse des communautés microbiennes associées aux graines lors de la transmission d'agents phytopathogènes Assembly of seed-associated microbial communities within and across successive plant generations Differences in stability of seed-associated microbial assemblages in response to invasion by phytopathogenic microorganisms." Thesis, Angers, 2017. http://www.theses.fr/2017ANGE0092.

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La graine constitue le point de départ du cycle d’une plante et abrite une diversité de micro-organismes qui peuvent impacter négativement ou positivement la fitness de la plante. De plus, la graine permet la dispersion et la survie des agents phytopathogènes entre deux cycles de culture de la plante hôte. Dans ce contexte, l’objectif de ce travail était de : (i) décrypter les processus écologiques impliqués dans l’acquisition du microbiote des graines, (ii) analyser sa réponse à l’invasion par des agents phytopathogènes et(iii) suivre sa dynamique durant la germination de la graine et l’émergence de la plantule. Premièrement, nous avons analysé la structure du microbiote de graines de radis(Raphanus sativus) produites dans un même site sur trois générations successives. Ces analyses ont révélé une faible héritabilité du microbiote des graines avec peu de taxons dominants transmis d’une génération à l’autre. Ceci pourrait être expliqué par l’importance des processus neutres dans l’assemblage du microbiote des graines.Ensuite, nous avons étudié la réponse de ce microbiote à une invasion par Xanthomonas campestris pv. campestris(Xcc) et Alternaria brassicicola (Ab), deux agents phytopathogènes transmis par les graines. La transmission de Xcc aux graines n’impacte pas la composition globale du microbiote. En revanche, la transmission d’Ab modifie la structure des communautés fongiques. Ces différences de réponse sont probablement dues aux compétitions pour l’espace et les nutriments entre l’agent phytopathogène et les autres membres du microbiote. Finalement, la composition et la structure du microbiote des graines germées et des plantules ont révélé une transmission de la majorité des taxons associés à la graine y compris Xcc etAb. Globalement, les résultats de ce travail de thèse permettront à terme d’élaborer des stratégies de biocontrôle basées sur la modulation du microbiote des graines<br>Seed represents the initial step of the plant life cycle and harbors diverse microorganisms that can have detrimental or beneficial impacts on plant fitness. Moreover, seed represents an important means of pathogen dispersion and survival during intercrop periods. For those reasons, the aims of this work were to (i) unveil the ecological processes involved in the acquisition of the seedmicrobiota, (ii) to analyze its response against plant pathogens invasion and (iii) to monitor its dynamics during the first plant developmental stages, namely germination and emergence. First, we assessed the structure of the radish seed microbiota (Raphanus sativus) in the same experimental site across three successive plant generations. These analyses revealed a low heritability of the seed microbiota with few dominant taxa transmitted across generations. Neutral-based processes seem to be important in assembly of the seed microbiota. Second, we monitored the response of the seed microbiota to invasions by Xanthomonas campestris pv. campestris (Xcc) and Alternaria brassicicola (Ab), two seed-transmitted pathogens. While Xcc seed transmission do not change the composition of microbial communities, Ab transmission modified the structure of seed-associated fungal communities. This differences in response could be partly explained by competition for space and nutrients between the pathogenic agents and the members of the seed microbiota. Finally, composition and structure of microbial communities associated to germinating seed and seedling revealed transmission of most seed-borne microorganisms including Xcc and Ab from seed to seedling. Altogether, the results of this thesis could be helpful for designing future biocontrol strategies based on seed microbiota modulation
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Luz, Leandro Vinícius da. "CARACTERIZAÇÃO CITOGENÉTICA E DE CRESCIMENTO DE Schinus terebinthifolius Raddi." Universidade Federal de Santa Maria, 2013. http://repositorio.ufsm.br/handle/1/4866.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Tropical forests play vital role in maintaining the stability and quality of the environment, protecting the soil and water resources, conserving the biodiversity, protecting cultural and recreational values, which contribute to improving the quality of life of the population. Being the germplasm of forest species a wealth to be used and preserved. Schinus terebinthifolius (Anacardiaceae) has medicinal, alimentary and ecological properties, besides being destined for the recomposition of degraded areas of permanent preservation. However, there are few studies on the cytogenetics characterization and promotion growth of this species. Thus, aiming for conservation and sustainable use, the objective is to characterize cytogenetically this species, assess the quality of the seeds S. terebinthifolius from individuals of different accessions of populations of Rio Grande do Sul, as well as, to evaluate the effect of the interaction of Trichoderma spp. on the contamination and in vitro germination and ex vitro vegetative growth of S. terebinthifolius. For the determination of chromosome number were used tissues of root tips. In the germination study, the parcels with seeds were kept in a climatic chamber with a photoperiod of 16 hours at the temperature of 20°C (+/- 2°C). To the study the interaction between S. terebinthifolius and Trichoderma spp. were evaluated the isolates TSM1 and TSM2 of Trichoderma viride, 2B2 and 2B22 of Trichoderma harzianum for the in vitro experiment, through the cellophane technique and the ex vitro experiment was conducted in a greenhouse, where were evaluated the effects of the isolates 2B2 and 2B22 and the commercial product Trichodermil®, all in the presence and absence of growth regulator Stimulate®. It is concluded that the chromosome number of the species S. terebinthifolius determined for 22 accessions collected in Rio Grande do Sul is 2n = 28, indicating that there isn't intraspecific variability. The quality of the seeds of S. terebinthifolius shows great heterogeneity among the different accessions collection. With the technique of in vitro cellophane, all four isolates of Trichoderma spp. tested are efficient in controlling fungal contamination of seeds. In ex vitro cultivation, the difference was significant growth in leaf area index for strain 2B2<br>As florestas tropicais desempenham função vital na manutenção da estabilidade e qualidade do meio ambiente, protegem o solo e os recursos hídricos, conservam a diversidade biológica, protegem os valores culturais e recreativos, que contribuem com a melhoria da qualidade de vida da população. Sendo o germoplasma de espécies florestais uma riqueza a ser utilizada e preservada. Schinus terebinthifolius (Anacardiaceae) possui propriedades medicinais, alimentícias e ecológicas, além de serem destinados à recomposição de áreas degradadas de preservação permanente. No entanto, ainda são poucos os estudos sobre a caracterização citogenética e promoção de crescimento dessa espécie. Assim, visando à conservação e a utilização sustentável, objetiva-se caracterizar citogeneticamente esta espécie, avaliar a qualidade das sementes de S. terebinthifolius provenientes de indivíduos de diferentes acessos do Rio Grande do Sul, bem como, avaliar o efeito da interação de Trichoderma spp. na contaminação e germinação in vitro e no crescimento vegetativo ex vitro de S. terebinthifolius. Para a determinação do número de cromossomos, foram utilizados tecidos de pontas de raízes. No estudo de germinação, as parcelas com as sementes foram mantidas em câmara climática com fotoperíodo de 16 horas à temperatura de 20°C (+/- 2°C). Para o estudo da interação entre S. terebinthifolius e Trichoderma spp. foram avaliados os isolados TSM1 e TSM2 de Trichoderma viride, 2B2 e 2B22 de Trichoderma harzianum para o experimento in vitro, através da técnica do papel celofane e o experimento ex vitro foi realizado em casa de vegetação, onde se avaliou os efeitos dos isolados 2B2 e 2B22, e o produto comercial Trichodermil®, todos na presença e ausência do regulador de crescimento Stimulate®. Conclui-se que o número de cromossomos da espécie S. terebinthifolius determinado para 22 acessos coletados no Rio Grande do Sul é de 2n = 28, indicando que não ocorre variabilidade intraespecífica. A qualidade das sementes de S. terebinthifolius apresenta grande heterogeneidade entre os diferentes acessos de coleta. Com a técnica in vitro do papel celofane, os quatro isolados de Trichoderma spp. testados são eficientes no controle da contaminação fúngica das sementes. No cultivo ex vitro, observou-se diferença significativa de crescimento no índice de área foliar para o isolado 2B2;
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Cherian, Jency. "Towards developing effective decontamination procedures for in vitro culture of embryonic axes excised from recalcitrant seeds." Thesis, 2013. http://hdl.handle.net/10413/10775.

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Control of seed-associated micro-organisms is vital in reducing losses of plants of economic importance. Recalcitrant seeds being metabolically active and able to be stored only under conditions of high relative humidity makes it more difficult to control contaminants. Nevertheless, means need to be developed to eliminate, or at least curtail, seed-associated fungi and bacteria. The use of biological control is a highly recommended alternative to chemical control for reducing the risk of killing beneficial organisms, as well as in terms of health and environmental hazards. Furthermore, when working with seed-derived tissues, it is extremely important to optimise a method or methods to control contamination without compromising the viability or further development of the explants. The original aim of the present study was to determine whether the biocontrol agents, EcoT® and Eco77® (commercial products of the spores of Trichoderma harzianum) would effectively control/eliminate micro-organisms from the embryonic axes of Trichilia dregeana, while promoting growth under in vitro conditions. Other means were also tested for their efficacy in controlling contaminants; these were application of Benlate®, Nipastat® (a mixture of parabens), anodic water (the anodic fraction of an electrolysed dilute solution of calcium and magnesium chloride), sodium dichloro-isocyanurate (Medi-Chlor®[NaDCC]) and alginate gel encapsulation of the embryonic axes. Prior to the experiments, fungal contaminants from the embryonic axes were isolated on potato dextrose medium and identified using light microscopy. EcoT and Eco77 were initially individually tested by co-culture as conidial suspensions with the embryonic axes. A further approach used liquid culture (potato dextrose broth) as well as solid culture medium (based on sugarcane bagasse) in/on which the strains of Trichoderma harzianum had been grown. This was aimed at testing for the possible presence of compounds released by T. harzianum into the media, which might prove to be effective in curtailing/eliminating the axis-associated microflora. Among the different treatments tested, the best method was utilised to decontaminate the embryonic axes prior to minimal-growth storage (hydrated axes encapsulated in alginate gel ‘beads’). Penicillium spp. were predominant among the different fungi isolated, which included Fusarium spp., Rhizopus spp., Aspergillus niger and Aspergillus flavus. Co-culturing with T. harzianum for 24 h was successful in terms of the survival of the embryonic axes, although the roots produced were shorter than when axes were cultured alone, but had no effect in eliminating the contaminants. Longer periods of co-culturing with T. harzianum affected the germination of the embryonic axes of T. dregeana compared with axes germinated in the absence of the biocontrol agent (control). The culture filtrate negatively affected germination of the T. dregeana embryonic axes, although it was effective against the associated contaminants. Nipastat was effective in reducing the contamination, and, depending on the concentration, did not affect germination adversely. Medi-Chlor was highly effective in eliminating all the contaminants from axes in vitro. Both these treatments were therefore used to decontaminate axes before minimal-growth storage. All the NaDCC-treated, encapsulated axes examined after 14 d hydrated storage [in Magenta boxes] and after 14-42 d in polythene bags survived; however the axes stored in aluminium foil-lined bags and Eppendorf tubes soon lost viability. The recommendation is therefore made that the decontamination treatment based on use of NaDCC (or other preparations of sodium dichloro-isocyanurate) be tested on embryonic axes of a range of recalcitrant-seeded species, and, if successful, the procedure be introduced into cryopreservation protocols. The use of NaDCC has emerged as a promising method of eliminating contaminating microflora which otherwise compromise in vitro procedures, from seed-derived explants. Furthermore, containment of decontaminated encapsulated axes in sealed polythene bags offers an apparently ideal means of temporary storage and dissemination. The results should find considerable applicability when excised embryonic axes representing the germplasm of recalcitrant seeds, are cryoconserved.<br>Thesis (M.Sc.)-University of KwaZulu-Natal, Durban, 2013.
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Rincón, Tomás Blanca. "Symbiotic adaptation of prokaryotic microorganisms in extreme deep-sea environments." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E601-2.

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Books on the topic "Microorganisms – Seeds"

1

Anderson, Robert Lee. Checklist of micro-organisms associated with tree seeds in the world, 1985. U.S. Dept. of Agriculture, Forest Service, Southeastern Forest Experiment Station, 1986.

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Anderson, Robert Lee. Checklist of micro-organisms associated with tree seeds in the world, 1985. U.S. Dept. of Agriculture, Forest Service, Southeastern Forest Experiment Station, 1986.

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Anderson, Robert Lee. Checklist of micro-organisms associated with tree seeds in the world, 1985. U.S. Dept. of Agriculture, Forest Service, Southeastern Forest Experiment Station, 1986.

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Anderson, Robert Lee. Checklist of micro-organisms associated with tree seeds in the world, 1985. U.S. Dept. of Agriculture, Forest Service, Southeastern Forest Experiment Station, 1986.

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Anderson, Robert Lee. Checklist of micro-organisms associated with tree seeds in the world, 1985. U.S. Dept. of Agriculture, Forest Service, Southeastern Forest Experiment Station, 1986.

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Agarwal, P. C. Seed-borne diseases and seed health testing of rice. Danish Government Institute of Seed Pathology for Developing Countries, 1989.

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Agarwal, P. C. Seed-borne diseases and seed health testing of rice. Danish Government Institute of Seed Pathology for Developing Countries, 1989.

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Agarwal, P. C. Seed-borne diseases and seed health testing of rice. Danish Government Institute of Seed Pathology for Developing Countries, 1989.

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Burges, Horace Denis. Formulations of microbial biopesticides: Beneficial microorganisms, nematodes and seed treatments. Chapman and Hall, 1998.

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Narayanasamy, Perumal. Microbial Plant Pathogens: Detection and Management in Seeds and Propagules. Wiley & Sons, Incorporated, John, 2016.

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Book chapters on the topic "Microorganisms – Seeds"

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McQuilken, Mark P., Peter Halmer, and David J. Rhodes. "Application of Microorganisms to Seeds." In Formulation of Microbial Biopesticides. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-4926-6_8.

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Stein, Tina, Joerg Ueckert, and Istvan Fendrik. "Establishment of Two Nitrogen-Fixing Bacteria on Roots of Kallar Grass Using Alginate-Coated Seeds in Mixed Inoculation." In Azospirillum VI and Related Microorganisms. Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79906-8_25.

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Halloin, John M. "Microorganisms and Seed Deterioration." In Physiology of Seed Deterioration. Crop Science Society of America, 2015. http://dx.doi.org/10.2135/cssaspecpub11.c5.

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Sreejith, S., R. Aswani, and E. K. Radhakrishnan. "Agriculturally Important Biosynthetic Features of Endophytic Microorganisms." In Seed Endophytes. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-10504-4_20.

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Ajilogba, Caroline Fadeke, Oluwaseyi Samuel Olanrewaju, and Olubukola Oluranti Babalola. "Application of Bioinoculants for Seed Quality Improvement." In Microorganisms for Sustainability. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6241-4_14.

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Marwal, Avinash, and R. K. Gaur. "Disease-Causing Seed Pathogenic Microorganisms and Their Management Practices." In Advances in Seed Production and Management. Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-4198-8_9.

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Xu, Huifang. "Synergistic Roles of Microorganisms in Mineral Precipitates Associated with Deep Sea Methane Seeps." In Geomicrobiology: Molecular and Environmental Perspective. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9204-5_15.

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Sanmartín, Patricia. "New Perspectives Against Biodeterioration Through Public Lighting." In Microorganisms in the Deterioration and Preservation of Cultural Heritage. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-69411-1_7.

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AbstractThere is currently an increasing trend in urban centres towards the use of public outdoor lighting systems to illuminate historic and architecturally important buildings during evening hours, but for which there is no specific regulatory framework. Considering that the light is a key factor involved in regulating growth and physiological processes in photosynthetic organisms, it seems appropriate to address the effects that artificial light has on the organisms growing on the facades affected by public lighting. In this sense, despite scientific research in the fields of biological colonization of buildings surfaces and light technology has advanced greatly in recent years, the combination of both disciplines aimed at the correct handling of city public lighting remains uncharted territory with huge potential to provide innovative solutions for smart cities. Recent studies have examined how urban monuments are affected by night-time outdoor illumination in combination with natural sunlight and demonstrated that the use of suitable lighting can inhibit the development of biological colonization. In this frame, this chapter will look at ways of contribute to the long-term management of public illumination on monuments and other structures, while reducing negative impacts caused by night lighting.
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Boulter, D., and A. Delauney. "The Structure of Plant Genes as Exemplified by Pea Seed Storage Protein Genes and their Expression in Microorganisms." In Perspectives in Biotechnology and Applied Microbiology. Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4321-6_24.

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Değirmencioğlu, Taşkın. "Use of Aromatic Seeds as Feed Additives to Improve the Production of Anatolian Water Buffaloes." In Veterinary Medicine and Science. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98999.

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Buffaloes have a strong population of rumen microorganisms that can utilize different feeds. Other features that make this animal unique are its compatibility with nature, its ability to withstand difficulties, and the nutrient richness of animal products. Today, the emergence of residues such as animal origin diseases, pesticides and antibiotics in animal food products has led organic products to be preferred more by consumers. Due to the increase in consumption demand of natural products, the share of buffalo products in the world market is gradually increasing. Focusing on the use of natural additives in buffalo feeding is important for the diversity of healthy products. Examples of natural additives are cumin and fenugreek seeds. In addition to being natural products, these seeds are reported to have versatile functions in the animal body. The purpose of this chapter is to show how to use aromatic seeds as feed additives in the feeding of Anatolian water buffaloes. The chapter also includes various articles on the use of aromatic seeds in buffalo rations in international fields. This review focuses on the current research relating to the use of aromatic seeds as feed additives to improve the production of Anatolian water buffaloes. Based on the scientific results presented in this chapter, authors drew the following main conclusions: (1) The use of aromatic seeds as feed additives of Anatolian water buffaloes feeding enabled in enhancing milk production, without changing the taste and chemical composition of the milk. (2) Milk production can be improved up to 0.67 kg and 0.85 kg day-1 by adding 50 g Fenugreek seeds and 30 g Cumin seeds to the ration of buffaloes in the early lactation period. (3) The use of aromatic seeds in the feeding of water buffaloes has been proven therefore, their use in the buffalo sector should be encouraged.
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Conference papers on the topic "Microorganisms – Seeds"

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Sviridova, O. V., N. I. Vorobyov, Ya V. Pukhalsky, et al. "Ability of the soil cellulolytic bacteria to colonize endophytic niche of barley grains." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.20.

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To identify microorganisms that can penetrate into the endophytic niche of the grain of barley plants, many years of vegetative experiments were conducted on sod-podzolic soil without the use of mineral fertilizers. In the non-growing season, a biological product, consisting of cellulolytic association of bacteria with genotypic passport, decomposed barley straw. Presowing treatment of seeds was not carried out, therefore, during the growing season; local microorganisms decomposing plant residues could be present in the barley rhizosphere. After six years of rotation of barley plants, the microbiological composition of its seed niche was studied. As a result, it was found that in the seeds of barley bacteria are present in an amount of 240 ± 20 CFU/g of grain. Isolated pure cultures of microorganisms were identified as Cellulomonas gelida, Micrococcus luteus and Bacillus licheniformis by the sequence of ITS fragments of 16S rRNA. These types of bacteria were also present in the used biological product. Based on the research conducted, it can be assumed, that permanent cultivation of barley plants and sowing of seeds of the previous year can contribute to the formation of effective microbial and plant biosystems that are resistant to environmental stress.
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Ali Demirci, Ratna R. Sharma, Larry R. Beuchat, and William F. Fett. "Application of Ozone for Inactivation of Pathogenic Microorganisms on Alfalfa Seeds and Sprouts." In 2002 Chicago, IL July 28-31, 2002. American Society of Agricultural and Biological Engineers, 2002. http://dx.doi.org/10.13031/2013.9716.

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Mantrova, M. V. "COMPARATIVE ASSESSMENT OF PHYSICAL AND CHEMICAL PARAMETERS, QUANTITATIVE COMPOSITION OF THE MAIN GROUPS OF MICROORGANISMS AND PHYTOTOXICITY OF CERTAIN TYPES OF SOILS IN SURGUT." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. DSTU-PRINT, 2020. http://dx.doi.org/10.23947/interagro.2020.1.704-708.

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The article presents a comparative analysis of 4 urban soils-culturosem, 2 urbanozem and undisturbed podzolic soil (control) - by physical and chemical parameters, the quantitative composition of the main groups of microorganisms and phytotoxicity. Urban soils differ from the background and from each other in terms of acidity, saturation of bases, and lead content; they lead in the number of microorganisms and have a stimulating effect on radish seeds and seedlings.
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Minich, A. S., I. B. Minich, A. E. Ivanitckii, M. K. Verhovod, I. D. Ivanova, and S. V. Gizbrekht. "RESPONSE GROWTH RESPONSES OF CUCUMIS SATIVUS PROPAGORS FOR PRECISIONING SEEDS OF SEEDS BY BARRIER DISCHARGE PLASMA." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-517-521.

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Lazukin, A. V., Y. A. Serdyukov, L. T. Saidova, A. I. Lilienberg, E. A. Kauer, and S. A. Krivov. "EFFECT OF PROCESSING OF SURFACE DISCHARGE OF SEEDS OF WINTER RYE ON MORPHOLOGICAL INDICATORS AND ANTIOXIDANT ENZYMES OF SEEDS BY PRODUCTS." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-458-461.

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Nizhnikov, A. A. "Amyloid proteins of plants and microorganisms: biological functions and participation in the formation of supra-organismal systems." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.184.

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Here we will review the latest advances in the study of functional amyloids of plants and symbiotic bacteria demonstrating the involvement of these protein fibrils in protein storage in plant seeds and formation of supra-organismal interactions.
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Morozov, G. A., N. Ye Stakhova, and Ya N. Shangaraeva. "Stimulating and inactivating effects of microwave processing on plant seeds and associated with them microflora and microorganisms." In 2013 IX International Conference on Antenna Theory and Techniques (ICATT). IEEE, 2013. http://dx.doi.org/10.1109/icatt.2013.6650689.

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Boldakov, D. M., E. R. Davoyan, R. O. Davoyan, Yu S. Zubanova, and A. A. Saushkina. "Search of a new donors of resistance to stem rust in lines of common wheat with genetic material of Agilops speltoides." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-20205-9-10-54.

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Scientific work is aimed at developing biotechnology for growing biologically safe products of Linum usitatissimum L. This research presents the study of the structure of microbocenosis in the rhizosphere of Linum usitatissimum L. under the influence of seed bacterization with new multifunctional cyanobacteria forms under conditions of southern Chernozem. In 2017, pre-sowing seed bacterization with strain Nostoc linckia 144 increased the number of aminotrophs by 1.4 times at the beginning of plant vegetation; in 2018 – by 3.3 times during their final stages of maturity. The number of micromycetes was tripled to the end of flax vegetation compared to control. In 2017, bacterization of seeds with a homogenate based on the strain Nostoc linckia 144 contributed to a 1.4-fold increase in the number of cellulose-decomposing microorganisms by the closing stages of the vegetation period.
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Alekseenko, O. P. "Influence of seed bacterization on the structure of microbocenosis of the southern Chernozem rhizosphere when growing Linum usitatissimum L." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-101.

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Scientific work is aimed at developing biotechnology for growing biologically safe products of Linum usitatissimum L. This research presents the study of the structure of microbocenosis in the rhizosphere of Linum usitatissimum L. under the influence of seed bacterization with new multifunctional cyanobacteria forms under conditions of southern Chernozem. In 2017, pre-sowing seed bacterization with strain Nostoc linckia 144 increased the number of aminotrophs by 1.4 times at the beginning of plant vegetation; in 2018 – by 3.3 times during their final stages of maturity. The number of micromycetes was tripled to the end of flax vegetation compared to control. In 2017, bacterization of seeds with a homogenate based on the strain Nostoc linckia 144 contributed to a 1.4-fold increase in the number of cellulose-decomposing microorganisms by the closing stages of the vegetation period.
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Azarkovich, M. I. "COLD STRESS TOLERANCE OF RECALCITRANT SEEDS: THE ROLE OF LEA-PROTEINS AND DEHYDRINS." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-43-46.

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