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1

da, Silva Carolina Prandi, Almeida Rafael Felipe de, Talita Kely Bellonzi, and Eduardo Custódio Gasparino. "Evolution of pollen grain morphology in Amorimia and allies evidences the importance of palynological apomorphies and homoplasies in Malpighiaceae systematics." Plant Ecology and Evolution 156, no. (3) (2023): 399–415. https://doi.org/10.5091/plecevo.102524.

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Background and aims – Pollen grain morphology is an important morphological character for aiding the systematics of flowering plants. For Malpighiaceae, only a single unpublished palynological study has comprehensively sampled ca 60 of this family's 75 currently accepted genera. To test the systematic relevance of pollen morphology in <i>Amorimia</i> and allies, we characterised the pollen morphology of these lineages. We scored, coded, and mapped 12 characters onto the most recent molecular phylogeny of <i>Amorimia</i> and allies. Material and methods – We sampled 13 species of <i>Amorimia</i
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2

Rosen, Arlene Miller. "Ancient Town and City Sites: A View from the Microscope." American Antiquity 54, no. 3 (1989): 564–78. http://dx.doi.org/10.2307/280783.

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Archaeological studies concerned with reconstructing activity areas, room functions, and site-formation processes can benefit greatly from analyses of the microartifacts found on and within occupation surfaces. These remains are often primary refuse directly related to activities, and can be used to identify such locations as food-preparation areas, flint-knapping stations, and storage facilities. In addition, certain microartifacts are informative about site-formation processes. For example, the grain-size distribution of charcoal may be indicative of primary vs. secondary refuse, high percen
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3

Kucheriavyi, Y. P. "TRANSMISSION ELECTRON MICROSCOPY FOR THE DIRECT ANALYSIS OF FIBRIN CLOT STRUCTURE." Biotechnologia Acta 16, no. 2 (2023): 30–31. http://dx.doi.org/10.15407/biotech16.02.030.

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The aim of our study was to compare the structure of clots formed as a result of thrombin-induced fibrin polymerization in the presence or absence of monoclonal fibrin-specific antibodies fragments as factors that change the clot structure. We concentrated on the final stage of fibrin clot formation at maximal turbidity point for every sample. Methods. Fibrin polymerization was studied by transmission electron microscopy (TEM) of negatively contrasted samples on H-600 Transmission Electron Microscope (“Hitachi”,Japan); 1% water solution of uranyl acetate (“Merck”, Germany) was used as a negati
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4

Demicheli, Romano, William Hrushesky, Michael Retsky, and Elia Biganzoli. "Interpreting Breast Cancer Survival Data by the Hazard Function: Remarkable Findings from Event Dynamics." Medicina 56, no. 9 (2020): 468. http://dx.doi.org/10.3390/medicina56090468.

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The report addresses the role of the hazard function in the analysis of disease-free survival data in breast cancer. An investigation on local recurrences after mastectomy provided evidence that uninterrupted growth is inconsistent with clinical findings and that tumor dormancy could be assumed as working hypothesis to understand the clinical course of the disease. Additionally, it was deemed that the lag-time between primary tumor removal and tumor recurrence is dynamically dependent on the subclinical metastasis development within the host-tumor system and, therefore, may be informative abou
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Iñiguez, Alena Mayo, Lorrayne Brito, Lucélia Guedes, and Sergio Augusto de Miranda Chaves. "Helminth infection and human mobility in sambaquis: Paleoparasitological, paleogenetic, and microremains investigations in Jabuticabeira II, Brazil (2890 ± 55 to 1805 ± 65 BP)." Holocene 32, no. 3 (2021): 200–207. http://dx.doi.org/10.1177/09596836211060490.

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Sambaquis or shellmounds are archeological sites constructed by hunter-fisher-gatherers that inhabited the Brazilian coast about 10,000–2000 yrs BP. Jabuticabeira II (JABII: 2890 ± 55 to 1805 ± 65 BP) is one of dozens of contemporaneous sambaquis of the Santa Catarina state, South Brazil, and contains hundreds of neatly organized burials, indicating great population density. In order to gather information about the health, diet and way of life of people in JABII, a paleoparasitological, paleogenetic, and micro-human remains investigation was carried out. Pelvic region and environmental control
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6

Carter, Francis, Alfred Anwander, Mathieu Johnson, et al. "Assessing quantitative MRI techniques using multimodal comparisons." PLOS One 20, no. 7 (2025): e0327828. https://doi.org/10.1371/journal.pone.0327828.

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The study of brain structure and change in neuroscience is commonly conducted using macroscopic morphological measures of the brain such as regional volume or cortical thickness, providing little insight into the microstructure and physiology of the brain. In contrast, quantitative Magnetic Resonance Imaging (MRI) allows the monitoring of microscopic brain change non-invasively in-vivo, and provides directly comparable values between tissues, regions, and individuals. To support the development and common use of qMRI for cognitive neuroscience, we analysed a set of qMRI and dMRI metrics (R1, R
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7

Thuy, Ben, Larry Knox, Lea D. Numberger-Thuy, Nicholas S. Smith, and Colin D. Sumrall. "Ancient deep ocean as a harbor of biotic innovation revealed by Carboniferous ophiuroid microfossils." Geology 51, no. 2 (2023): 157–61. http://dx.doi.org/10.1130/g050596.1.

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Abstract Fossil-informed molecular phylogenies have emerged as the most powerful tool for correlating biotic evolution and Earth history. The accuracy of these trees, however, depends on the completeness of fossil sampling. For most organismal clades, the available fossil record is insufficiently sampled. This is especially true for groups with a multi-element skeleton, such as echinoderms and vertebrates, where sampling efforts focus largely on rare finds of intact skeletons. For these groups, inconspicuous but informative skeletal fragments are commonly neglected. This sampling bias excludes
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8

Boccacci, Giulia, Francesca Frasca, Chiara Bertolin, and Anna Maria Siani. "Influencing Factors in Acoustic Emission Detection: A Literature Review Focusing on Grain Angle and High/Low Tree Ring Density of Scots Pine." Applied Sciences 12, no. 2 (2022): 859. http://dx.doi.org/10.3390/app12020859.

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Among non-destructive testing (NDT) techniques applied to structural health monitoring in existing timber structures, ranging from visual inspection to more sophisticated analysis, acoustic emission (AE) is currently seldomly used to detect mechanical stresses in wooden building assets. This paper presents the results from a systematic literature review on AE NDT applied to monitor micro and macro fracture events in softwood, specifically Scots pine. This survey particularly investigates its application with respect to the tree rings density and grain angle inspection, as influencing factors w
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9

Burykina, S. I., S. P. Uzhevska, and N. V. Pylyak. "METHOD OF ASSESSING THE NEMATOCIDAL EFFECTIVENESS OF A BIOPREPA-RATION BASED ON PREDATORY MICROMYCETE AGAINST THE POTATO STEM NEMATODE." Microbiology&Biotechnology, no. 3(59) (December 20, 2023): 14–25. http://dx.doi.org/10.18524/2307-4663.2023.3(59).292392.

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Aim – Studing of methods for determining the effectiveness of Nematophagin BT against potato stem nematode in the laboratory. Materials and methods. For the experiment, potato tubers with signs of damage by the stem nematode Ditylenchus destructor Thorne, 1945 were selected. The method of treatment of cut potato tubers with the preparation was used. It was compared with the method of treatment of whole tubers in the laboratory. The effect of the microbiological nematicidal preparation (Nematofagin BT) developed by ITI Biotechnology of NAAS was determined. The effectiveness of the microbiologic
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10

Zeleneva, Yu V., Ph B. Gannibal, I. А. Kazartsev, and V. P. Sudnikova. "Molecular Identification, Effector genes and Virulence of Isolates of <i>Parastagonospora nodorum</i> from Altai Krai (Russia)." Микология и фитопатология 57, no. 5 (2023): 362–71. http://dx.doi.org/10.31857/s0026364823050124.

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Septoria blotches represent some of the most harmful wheat diseases that encompass all grain-producing countries worldwide. They are caused mainly by fungi of the genera Parastagonospora and Zymoseptoria. The aims of the research were to analyze the nucleotide sequences of two phylogenetically informative DNA loci, ITS and tub2, of Parastagonospora nodorum isolates from the Altai Krai, to detect the presence of Tox1, Tox3, ToxA effector genes and to study the virulence of the isolates. Microscopic analysis of fragments of affected plant tissue was used for primary identification of the Septori
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11

Sokol, Ye I., K. V. Kolisnyk, and T. V. Bernads’ka. "Evaluation of metrological characteristics of spectral analysis method for determining erythrocyte morphology." Технология и конструирование в электронной аппаратуре, no. 3-4 (2021): 45–49. http://dx.doi.org/10.15222/tkea2021.3-4.45.

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Spectral photometry is currently widely used for quantitative and qualitative analysis of biological molecules in medical biology. The method is based on the ability of molecules to absorb electromagnetic radiation. Modern clinical laboratory diagnostics extensively uses optical methods of analysis that rely on these physical properties of semitransparent objects, such as blood components. Knowing the absorption spectra of blood and its components, it is possible to quantify the concentration of all the components by solving the mathematical system of equations corresponding to these spectra.
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12

Drozd, O. M., M. Yu Zhuravel, O. Ye Naidionova, I. P. Lezhenina, and N. Yu Polchaninova. "THE DETERMINATION OF LOCAL CHANGES IN THE SUPPORTING ECOSYSTEM SERVICE OF SOIL ON THE TERRITORY OF OIL AND GAS PRODUCTION." Ecological Safety and Balanced Use of Resources, no. 2(18) (December 3, 2018): 15–25. http://dx.doi.org/10.31471/2415-3184-2018-2(18)-15-25.

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The temporary withdrawal of agricultural land for the construction or overhaul of wells and pipelines laying is, in essence, an interference with the natural soil-forming process, the result of which is a change in the implementation of the ecosystem functions of the soil and, consequently, ecosystem services of the soil.&#x0D; The article analyses the influence of industrial activity and soils reclamation on the implementation of the supporting ecosystem service of soil. Such indicators as agrophysical, physico-chemical, chemical indices of soil, species composition, trophic structure and the
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13

Oliveira, P. P. T., and Eduardo Galembeck. "Applying a Design-Based Research Framework in the Production of Web Content to Disseminate the Remote Laboratory Uses and Development." Revista de Ensino de Bioquímica 17 (September 13, 2019): 112. http://dx.doi.org/10.16923/reb.v17i0.892.

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INTRODUCTION. The adoption of teaching methods that highlight student's autonomy is a long term process and requires students to be involved with hands-on activities such as lab classes. Lab activities can vary in methodological approaches from demonstration to the authentic research, and requires the use of equipment, reagents, and time. A lot of low-cost experiments alternatives have been developed as an alternative to the expensive equipment not always available in didactic labs. The availability of low-cost microcontrollers and sensors has made possible not only having students do run expe
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14

Ueno, Hideki, Megumi Ishiguro, Eiji Nakatani, et al. "Prognostic impact of tumor budding in stage II colon cancer: A prospective study (SACURA trial)." Journal of Clinical Oncology 35, no. 15_suppl (2017): 3609. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.3609.

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3609 Background: Growing number of studies indicate tumor budding is a significant prognostic factor in colorectal cancer (van Wyk, et al. Cancer Treat Rev 2015), but this has been shown only in retrospective studies. We prospectively evaluated prognostic factors in stage II colon cancer to determine their prognostic value in a multi-institutional phase III study (SACURA trial, ASCO2016 abst#3617). Methods: A total of 991 patients with curatively resected stage II colon cancer (2006–2010; 136 institutions) were included in the study. Tumor budding was defined as an isolated cancer cell or a cl
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15

Grygorian, E., V. Olkhovsky, M. Gubin, and V. Shishkin. "FORENSIC MEDICAL ASSESSMENT OF MORPHOLOGICAL CHANGES AT DIFFERENT POSTMORTEM INTERVAL." Inter Collegas 8, no. 3 (2021): 177–81. http://dx.doi.org/10.35339/ic.8.3.177-181.

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Purpose: The postmortem interval (PMI) evaluation is one of priorities while performing a forensic medical examination of corpse. To date, there is lack of information of morphological postmortem changes of some internal organs. Considering the persistent need to develop the method for a precise assessment of PMI, postmortem changes in these potentially informative organs were evaluated. The aim of study was to analyze morphological postmortem changes in prostate and uterus. Materials and Methods: histological samples of 40 prostate tissues and 40 uterus (n=80) from corpses of deceased aged 18
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16

Williamson, Helen, Edward Mountjoy, Hashem Shihab, et al. "Development Of Minimal Residual Disease (MRD) Analysis In Childhood Acute Lymphoblastic Leukaemia (ALL) By Next Generation Sequencing (NGS)." Blood 122, no. 21 (2013): 2569. http://dx.doi.org/10.1182/blood.v122.21.2569.2569.

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Abstract Detection of sub-microscopic levels of disease (minimal residual disease; MRD) in childhood acute lymphoblastic leukaemia (ALL) during treatment is an important prognostic factor. Currently, stratification of therapy for the new frontline trial in childhood ALL (UKALL 2011) is provided by MRD analysis using real time quantitative PCR (RQ-PCR) to identify and quantitate the patient specific rearrangements of the immunoglobulin (Ig) and T-cell receptor (TCR) genes. The current methodology is expensive, time-consuming and complex to perform. Although MRD has proven to be a powerful and e
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17

Mittal, Kush Mohan, Marc Timme, and Malte Schröder. "Efficient self-organization of informal public transport networks." Nature Communications 15, no. 1 (2024). http://dx.doi.org/10.1038/s41467-024-49193-1.

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AbstractThe Global South, encompassing more than 80% of the world population, heavily relies on informal paratransit services with ad-hoc routes. Yet, it remains unclear how efficiently such informal public transport services organize and operate. Here, we analyze and compare the structural efficiency of more than 7000 formal and informal bus service routes in 36 cities across 22 countries globally. Intriguingly, informal transport self-organizes in ways at or above efficiency levels of centralized services. They exhibit fewer detours, more uniform paths, and comparable interconnectivities, al
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18

Villeneuve, Thomas, Grégoire Prévot, Grégory Pugnet, et al. "Role of bronchoscopy for respiratory involvement in granulomatosis with polyangiitis and microscopic polyangiitis." ERJ Open Research, August 31, 2023, 00141–2023. http://dx.doi.org/10.1183/23120541.00141-2023.

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ObjectivesThis study describes data from bronchoscopy performed at the diagnosis of ANCA-associated vasculitis (AAV).MethodsWe conducted a retrospective study between 2004 and 2019 in patients older than 18 years old with a diagnosis of microscopic polyangiitis (MPA) or granulomatosis with polyangiitis (GPA) who underwent bronchoscopy at onset of the disease. We collected bronchoalveolar lavage (BAL) and histological findings obtained during bronchoscopy.ResultsTwo hundred and seventy-four patients with AAV were identified. Among 92 bronchoscopies, 62 were performed at diagnosis and 58 procedu
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19

Choi, Bokyung, Yeojin Hwang, Scott A. M. McAdam, and Tae‐Soo Jang. "Comparative microscopic investigations of leaf epidermis in four Ajuga species from Korea." Microscopy Research and Technique, November 2023. http://dx.doi.org/10.1002/jemt.24450.

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AbstractThe genus Ajuga is widely distributed in temperate to subtropical regions, and four species are currently recognized in Korea (A. decumbens, A. multiflora, A. nipponensis, and A. spectabilis), but epidermal anatomical differences across these species have never been described. A comparative study of the leaf micromorphological characteristics of Korean Ajuga species was performed using light microscopy (LM) and scanning electron microscopy (SEM) to elucidate their taxonomic usefulness and to assess leaf micromorphological diversity. Considerable diversity in epidermal and stomatal anat
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Ustinova, Anita, Ekaterina Volkova, Anastasiya Rakovskaya, Daria Smirnova, Olesya Korovina, and Ekaterina Pchitskaya. "Generate and Analyze Three‐Dimensional Dendritic Spine Morphology Datasets With SpineTool Software." Current Protocols 4, no. 12 (2024). https://doi.org/10.1002/cpz1.70061.

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AbstractDendritic spine morphology is associated with the current state of the synapse and neuron, and changes during synaptic plasticity in response to stimulus. At the same time, dendritic spine alterations are reported during various neurodegenerative and neurodevelopmental disorders and other brain states. Accurate and informative analysis of spine shape has an urgent need for studying the synaptic processes and molecular pathways in normal and pathological conditions, and for testing synapto‐protective strategies during preclinical studies. Primary neuronal cultures enable high quality im
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21

Musa, Sandrine, Theo Hemberle, Staffan Bensch, et al. "Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites." Frontiers in Cellular and Infection Microbiology 14 (April 29, 2024). http://dx.doi.org/10.3389/fcimb.2024.1385599.

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Avian haemosporidian parasites are useful model organisms to study the ecology and evolution of parasite-host interactions due to their global distribution and extensive biodiversity. Detection of these parasites has evolved from microscopic examination to PCR-based methods, with the mitochondrial cytochrome b gene serving as barcoding region. However, standard PCR protocols used for screening and identification purposes have limitations in detecting mixed infections and generating phylogenetically informative data due to short amplicon lengths. To address these issues, we developed a novel ge
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22

Thuy, Ben, Larry Knox, Lea D. Numberger-Thuy, Nicholas S. Smith, and Colin D. Sumrall. "Ancient deep ocean as a harbor of biotic innovation revealed by Carboniferous ophiuroid microfossils." Geology, January 3, 2023. http://dx.doi.org/10.1130/g50596.1.

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Fossil-informed molecular phylogenies have emerged as the most powerful tool for correlating biotic evolution and Earth history. The accuracy of these trees, however, depends on the completeness of fossil sampling. For most organismal clades, the available fossil record is insufficiently sampled. This is especially true for groups with a multi-element skeleton, such as echinoderms and vertebrates, where sampling efforts focus largely on rare finds of intact skeletons. For these groups, inconspicuous but informative skeletal fragments are commonly neglected. This sampling bias excludes the nume
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23

Boccacci, Giulia, Francesca Frasca, Chiara Bertolin, and Maria Siani Anna. "Influencing Factors in Acoustic Emission Detection: A Literature Review Focusing on Grain Angle and High/Low Tree Ring Density of Scots Pine." Applied Sciences 12, no. 2 (2022). https://doi.org/10.5281/zenodo.6807319.

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Among non‐destructive testing (NDT) techniques applied to structural health monitoring in existing timber structures, ranging from visual inspection to more sophisticated analysis, acoustic emission (AE) is currently seldomly used to detect mechanical stresses in wooden building assets. This paper presents the results from a systematic literature review on AE NDT applied to monitor micro and macro fracture events in softwood, specifically Scots pine. This survey particularly investigates its application with respect to the tree rings density and grain angle inspection, as influencing factors w
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24

Persson, Dennis Krog, Kenneth Agerlin Halberg, Ricardo Cardoso Neves, Aslak Jørgensen, Reinhardt Møbjerg Kristensen, and Nadja Møbjerg. "Comparative myoanatomy of Tardigrada: new insights from the heterotardigrades Actinarctus doryphorus (Tanarctidae) and Echiniscoides sigismundi (Echiniscoididae)." BMC Evolutionary Biology 19, no. 1 (2019). http://dx.doi.org/10.1186/s12862-019-1527-8.

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Abstract Background Tardigrada is a group of microscopic invertebrates distributed worldwide in permanent and temporal aquatic habitats. Famous for their extreme stress tolerance, tardigrades are also of interest due to their close relationship with Arthropoda and Cycloneuralia. Despite recent efforts in analyzing the musculature of a number of tardigrade species, data on the class Heterotardigrada remain scarce. Aiming to expand the current morphological framework, and to promote the use of muscular body plans in elucidating tardigrade phylogeny, the myoanatomy of two heterotardigrades, Actin
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Orsulic, Sandra, Joshi John, Ann E. Walts, and Arkadiusz Gertych. "Computational pathology in ovarian cancer." Frontiers in Oncology 12 (July 29, 2022). http://dx.doi.org/10.3389/fonc.2022.924945.

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Histopathologic evaluations of tissue sections are key to diagnosing and managing ovarian cancer. Pathologists empirically assess and integrate visual information, such as cellular density, nuclear atypia, mitotic figures, architectural growth patterns, and higher-order patterns, to determine the tumor type and grade, which guides oncologists in selecting appropriate treatment options. Latent data embedded in pathology slides can be extracted using computational imaging. Computers can analyze digital slide images to simultaneously quantify thousands of features, some of which are visible with
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Rotmuenwai, Naritsa, Ketsara Aryuyo, Nuttida Kruethaworn, Witoon Wattananit, and Nimnara Yookongkaew. "Exploring silica accumulation in bamboo leaves: a study on phytolith morphology and epidermal patterning in the tropical giant bamboo Dendrocalamus copelandii." Annals of Botany, December 2, 2024. https://doi.org/10.1093/aob/mcae209.

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Abstract Background and Aims Bamboo is a grass in the Poaceae family with various applications. Bamboo leaves can accumulate high silica. However, silica deposition in bamboo has received limited study. Therefore, this research investigated silica accumulation in Dendrocalamus copelandii leaves. The study includes the localization of silica through phytolith morphology, examination of the distribution patterns of phytoliths in epidermal tissues, analysis of silica accumulation within specialized silica cells (short cells) and analysis of silicon concentration across various leaf developmental
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27

Mendik, Caitlyn. "Redesigning Peppers RNA Imaging System." University of Colorado Honors Journal, April 30, 2024. http://dx.doi.org/10.33011/cuhj20242421.

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Abstract RNA is a central component of biochemistry as it carries genetic information, has enzymatic activity, mediates protein biodiversity, and supports other cellular functions. Scientists are continually developing new tools for studying RNA and applying it to fields of research such as medicine and molecular biology. RNA imaging provides one avenue for characterizing RNA by looking at trafficking, localization, and lifetime in living cells. Imaging tools have been on the rise in the past 20 years, providing innovative methods for looking at this nucleic acid under a microscope. Peppers is
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