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Journal articles on the topic 'Microsporum canis'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Lavrushko, S. I., and V. I. Stepanenko. "Modern diagnostics of microsporia." Ukrainian Journal of Dermatology, Venerology, Cosmetology, no. 2 (June 29, 2021): 16–24. http://dx.doi.org/10.30978/ujdvk2021-2-16.

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Objective — to develop a method of modern molecular genetic diagnosis of microsporia in children based on polymerase chain reaction (PCR), which will allow identification of the pathogen of Microsporum canis at the DNA level. Materials and methods. The study included 40 patients with microsporia of smooth skin, scalp, scalp and smooth skin. The biological materials for the research were scales from the smooth skin and scalp, hair from the scalp of patients with microsporia. A study of 40 samples of biological material was carried out in patients with microsporia of smooth skin, microsporia of the scalp, microsporia of the scalp and smooth skin. At the first stage, DNA isolation of Microsporum canis was carried out. Then PCR was carried out to increase the copies of the DNA region using specific primers. The final step was typing 40 samples of clinical material of patients. Results and discussion. PCR diagnostics made it possible to identify the DNA of Microsporum canis in all 40 samples of biological material of patients with microsporia. In our study, we developed a PCR-based method for diagnosing microsporia, which uses a set of two MC primers (regions of the beta tubulin gene of Microsporum canis). For internal control of the course of amplification and the quality of biomaterial sampling, specific primers of APOE (a region of the human apolipoprotein E gene) were also used. Conclusions. In order to improve the precise specific diagnosis of microsporia in children, a method of modern molecular genetic diagnostics based on polymerase chain reaction (PCR) has been developed, which allows identification of the Microsporum canis pathogen at the DNA level. Analysis of the molecular structure of the genome of Microsporum canis proved that the most objective diagnosis of microorganisms is the PCR method. The developed method of DNA diagnostics based on PCR using specific primers can be included in the algorithm for detecting Microsporum canis in humans.

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2

Uhrlaß, S., C. Krüger, and P. Nenoff. "Microsporum canis." Der Hautarzt 66, no. 11 (October 7, 2015): 855–62. http://dx.doi.org/10.1007/s00105-015-3697-7.

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3

Barboza-Quintana, Oralia, Raquel Garza-Guajardo, Carlos Assad-Morel, and Nora Méndez-Olvera. "Pseudomycetoma for Microsporum canis." Acta Cytologica 51, no. 3 (2007): 424–28. http://dx.doi.org/10.1159/000325759.

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4

Mancianti, Francesca. "Comments on Microsporum canis." Medical Mycology 36, no. 4 (August 1998): 247. http://dx.doi.org/10.1046/j.1365-280x.1998.00140.x.

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5

Mancianti, Francesca. "Comments on Microsporum canis." Medical Mycology 36, no. 4 (July 25, 2008): 247. http://dx.doi.org/10.1111/j.1365-280x.1998.00140.x.

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6

Difonzo, E. M., G. M. Palleschi, P. Vannini, R. Guadagni, and E. Panconesi. "Microsporum canis Epidemic in Laboratory Mice: Epidemie durch Microsporum canis bei Laboratoriumsmäusen." Mycoses 29, no. 12 (April 24, 2009): 591–95. http://dx.doi.org/10.1111/j.1439-0507.1986.tb04368.x.

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7

Nabwiyah, Ika Rofiqotun, Lilis Majidah, and Hindyah Ike Suhariati. "IDENTIFIKASI Microsporum canis PADA KUCING LIAR (Studi di Dusun Ringin Pitu Jogoroto Jombang)." Jurnal Insan Cendekia 7, no. 1 (March 9, 2020): 53–56. http://dx.doi.org/10.35874/jic.v7i1.557.

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Pendahuluan:Dermatofitosis merupakan penyakit zoonis yang disebabkan oleh kapang yang tergolong dalam genus dermatofita, dan pada hewan lebih dikenal dengan penyakit Ringworm. Penyakit ini disebabkan oleh kapang yang dikenal dengan nama Microsporum canis. Kucing merupakan hewan karnivora dan hewan predator kecil yang termasuk dalam mamalia crepuscular yang telah dijinakkan oleh manusia.Tujuan penelitian adalah untuk mengidentifikasi jamur Microsporum canis pada tubuh kucing liar di Dusun Ringin Pitu. Metode penelitian ini menggunakan metode deskriptif, dengan mengunakan teknik total sampling, sampel yang digunakan berjumlah 7 kerokan kulit kucing liar, variable dalam penelitian ini adalah jamur Microsporum canis, instrument yang digunakan yaitu microskopis dan pada penelitian ini mengunakan analisa data Editing, Coding, dan Tabulating. Hasil : Penelitian pada kerokan kulit kucing di Dusun Ringin Pitu menunjukkan bahwa 5 (60%) sampel kerokan kulit positif terinfeksi jamur Microsporum canis dan 2 (40%) sampel kerokan kulit negatif. Kesimpulan:Dapat disimpulkan bahwa sebagian kecil kucing liar di Dusun Ringin Pitu positif terinfeksi jamur Microsporum canis. Saran: Diharapkan kepada masyarakat untuk lebih memperhatikan kebersihan lingkungan kandang kucing agar terhindar dari spora jamur maupun bakteri yang dapat menginfeksi pada tubuh kucing.

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8

Sharp, M., G. Lupson, and M. Flamank. "Microsporum canis infection in sheep." Veterinary Record 132, no. 15 (April 10, 1993): 388. http://dx.doi.org/10.1136/vr.132.15.388.

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9

Brasch, J. "Microsporum canis with Polymorphous Macroconidia." Mycoses 32, no. 1 (April 24, 2009): 33–38. http://dx.doi.org/10.1111/j.1439-0507.1989.tb02167.x.

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10

Essayag, S. Mata. "An atypical Microsporum canis isolate." Mycoses 34, no. 11-12 (April 24, 2009): 505–11. http://dx.doi.org/10.1111/j.1439-0507.1991.tb00868.x.

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11

Danielli, L. J., W. Lopes, M. H. Vainstein, A. M. Fuentefria, and M. A. Apel. "Biofilm formation by Microsporum canis." Clinical Microbiology and Infection 23, no. 12 (December 2017): 941–42. http://dx.doi.org/10.1016/j.cmi.2017.06.006.

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12

Rodriguez-Contreras, P. R., F. V. Delgado, M. D. Ayudarte, E. A. Cueto, and V. R. Galvez. "Nosocomial infections with Microsporum canis." Journal of Hospital Infection 9, no. 2 (March 1987): 201–2. http://dx.doi.org/10.1016/0195-6701(87)90061-2.

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13

Kisera, Ia V., and Yu V. Martyniv. "The selection of the concentration of clotrimazole and povidone-iodineas the main active substances of the “Micromar” antifungal agent." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 21, no. 95 (November 2, 2019): 27–31. http://dx.doi.org/10.32718/nvlvet9505.

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Microsporia is one of the most common skin diseases of cats, most often provoked by the pathogen Microsporum canis. The pathogen of microsporium has highly contagious properties and for a long time remains capable of infection in the environment. It is important for the veterinary doctor not only to carry out complex therapy, but also to prevent the spread of the pathogen in the environment and to prevent the occurrence of secondary pyoderma during the course of microspores. Due to the tendency to the growth of fungal diseases, the development of the pathogen’s resistance existing medicines, there is a need for effective antifungal medicines that have fungicide and fungicidal effect. The development of the antifungal agent “Micromar” will allow to carry out complex treatments for the treatment of microspores in cats. Also, thanks to the properties of basic active ingredient? Th use of “Micromar” will provide acceleration of recovery period. The combination of clotrimazole and povidone iodine will provide an effective antifungal action with an antiseptic effect. Clotrimazole is a broad-spectrum antifungal agent that does not cause pathogen resistance. In turn, povidone iodine will provide antiseptic protection to the affected area of the cat's body with a prolonged effect. The studies were conducted to determine the concentration of clotrimazole and povidone iodine as the main active substances of the antifungal agent “Micromar” in the laboratory in nutrient media during the cultivation of the fungus Microsporum sanis. The results of the studies showed that the pure culture of the pathogen is sensitive to clotrimazole at a concentration of 0.25% and iodine povidone 5%. In appropriate concentrations, it is recommended to use clotrimazole and povidone iodine for the manufacture of the antifungal agent Micromar.

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14

Hecke, E., and L. Meysman. "Tinea capitis in an Adult (Microsporum canis): Tinea capitis durch Microsporum canis bei einem Erwachsenen." Mycoses 23, no. 11 (April 24, 2009): 607–8. http://dx.doi.org/10.1111/j.1439-0507.1980.tb02569.x.

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15

Rezaei-Matehkolaei, Ali, Koichi Makimura, G. Sybren de Hoog, Mohammad Reza Shidfar, Kazuo Satoh, Mohammad Javad Najafzadeh, and Hossein Mirhendi. "Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii." Journal of Medical Microbiology 61, no. 1 (January 1, 2012): 57–63. http://dx.doi.org/10.1099/jmm.0.036541-0.

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16

Costa, E. O., L. S. M. Diniz, N. R. Benites, S. D. Coutinho, V. M. Carvalho, L. F. Dutra, and E. G. Serra. "Surtos interespecíficos de dermatomicoses por Microsporum canis e Microsporum gypseum." Revista de Saúde Pública 28, no. 5 (October 1994): 337–40. http://dx.doi.org/10.1590/s0034-89101994000500005.

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As dermatomicoses dos animais domésticos constituem zoonoses importantes, urna vez que estes mantêm estreito contato com a espécie humana, dada a alta infectividade observada nesses processos. Relata-se a ocorrência de sete surtos de dermatomicoses, um por M. gypseum envolvendo um gato e um indivíduo do sexo feminino e os outros por M. canis envolvendo 20 indivíduos da espécie humana (adultos, jovens e crianças de ambos os sexos), 5 cães, 16 gatos e um macaco gibão (Hylobates lar).

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17

Brouta, Frédéric, Frédéric Descamps, Michel Monod, Sandy Vermout, Bertrand Losson, and Bernard Mignon. "Secreted Metalloprotease Gene Family of Microsporum canis." Infection and Immunity 70, no. 10 (October 2002): 5676–83. http://dx.doi.org/10.1128/iai.70.10.5676-5683.2002.

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ABSTRACT Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes.

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18

Rybnikář, A., V. Vrzal, J. Chumela, and J. Petráš. "Immunization of Cats Against Microsporum canis." Acta Veterinaria Brno 66, no. 3 (1997): 177–81. http://dx.doi.org/10.2754/avb199766030177.

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19

Amano, Hiroo, Chikako Kishi, Yoko Yokoyama, Akira Shimizu, Kazushi Anzawa, Takashi Mochizuki, and Osamu Ishikawa. "Microsporum canis infection mimics pemphigus erythematosus." Indian Journal of Dermatology 58, no. 3 (2013): 243. http://dx.doi.org/10.4103/0019-5154.110866.

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20

Souissi, Asmahane, Nourchène Toukabri, Fatma Jendoubi, Yosra Jmour, and Mourad Mokni. "Tinea ciliaris due to Microsporum canis." La Presse Médicale 47, no. 11-12 (November 2018): 1030–32. http://dx.doi.org/10.1016/j.lpm.2018.08.012.

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21

Mignon, Bernard. "Response to 'Comments on Microsporum canis'." Medical Mycology 36, no. 4 (August 1998): 248. http://dx.doi.org/10.1046/j.1365-280x.1998.00141.x.

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22

Maraki, S., G. Minadakis, M. Dialynas, E. Nioti, G. Samonis, and Y. Tselentis. "MICROSPORUM CANIS INFECTIONS IN CRETE, GREECE." Mycoses 45, S2 (August 2002): 37. http://dx.doi.org/10.1111/j.1439-0507.2002.tb04659.x.

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23

Mignon, Bernard. "Response to ‘Comments on Microsporum canis’." Medical Mycology 36, no. 4 (July 25, 2008): 248. http://dx.doi.org/10.1111/j.1365-280x.1998.00141.x.

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24

Bardazzi, F., I. Neri, S. Marzaduri, C. Landi, and A. D'Antuono. "Microsporum canis infection of the penis." Sexually Transmitted Infections 73, no. 6 (December 1, 1997): 579. http://dx.doi.org/10.1136/sti.73.6.579.

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25

ZIEGLER, H., and G. REICHMANN. "Über den Schwefelstoffwechsel von Microsporum canis*." Mycoses 11, no. 12 (April 24, 2009): 903–7. http://dx.doi.org/10.1111/j.1439-0507.1968.tb04458.x.

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26

Sonck, C. E. "Microsporum canis infections in SW-Finland." Mycoses 13, no. 1 (April 24, 2009): 49–59. http://dx.doi.org/10.1111/j.1439-0507.1970.tb01156.x.

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27

Pereira, Keylla Helena Nobre Pacífico, Elton Luís Ritir Oliveira, Raphael Augusto Baldissera Gonçalves, Luna Scarpari Rolim, Ramiro Das Neves Dias Neto, Maíra Sales Castilho, Carlos Roberto Teixeira, and Sheila Canavese Rahal. "Dermatophytosis Caused by Microsporum canis in a Free-Living Maned Wolf (Chrysocyon brachyurus)." Acta Scientiae Veterinariae 46 (February 16, 2018): 4. http://dx.doi.org/10.22456/1679-9216.86218.

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Background: Chrysocyon brachyurus is a South American wild canid considered a species near threatened by the International Union for Conservation of Nature and is classified as vulnerable in the National List of Endangered Species. With the increase of the contact between domestic animals, human population and wild animals, there was a greater exposure of the maned wolf to pathogens. Due to the importance of its conservation, the knowledge of emerging infectious diseases that affect this species becomes essential. This report aims to describe the first diagnosed case of dermatophytosis caused by Microsporum canis in a maned wolf.Case: A free-living female maned wolf (Chrysocyon brachyurus), approximately 60 days old, was rescued with presence of alopecia, non-pruritic lesions, one of circular shape located in the nasal plane and the other with signs of scaling and crusts in the region of the left pina. The animal was active, in good general condition and without other significantchanges to clinical examination. Skin scraping was performed for mycological culture of both lesions. Fungal growth on Sabouraud’s agar identified Microsporum canis. Topical therapy with ketoconazole ointment and cleaning of lesions with 0.2% chlorhexidine was instituted. After 20 days of treatment, remission of clinical signs and repilation of affected areas were observed. New mycological cultures of both areas were carried out, which were negative for Microsporum canis.Discussion: Although Microsporum canis is described as causing dermatophytosis in several animal species, it has apparently not yet been reported in maned wolves. Microsporum canis is one of the most isolated zoophilic dermatophytes in domestic cats and is also cited in reports of symptomatic wild felids, such as tigers, in which it has been identified as either the only agent of infection or in association with Trichophyton mentagrophytes. The occurrence is also high in domestic dogs. In the wild canids, Microsporum gypseum has been described in the gray wolf, Trichophyton mentagrophytes in wild foxes, and Microsporum canis in red fox, among others. The finding of zoophilic and geophilic dermatophyte fungi in healthy, free-living and captive wild animals confirms their role as carriers, probable reservoirs and disseminators of these organisms in the environment, and the potential risk they represent as causes of zoonoses. Zoophilic dermatophytes are usually transmitted by contact between individuals and contaminated fomites. In the case of M. canis in the red fox the contact was an asymptomatic domestic cat. In the present case the transmission was not determined, since the animal was rescued from the wild. Regarding the age group, young animals are more susceptible to dermatophytes than adults, probably related to the greater immunity of adults due to previous contacts or even the immaturity of the immune system of the young. The dermatophytosis diagnosis is generally based on anamnesis, physical examination of the lesion, Wood’s lamp examination, microscopic skin scraping, fungal culture, or even histology and PCR tests. In the present case, the final diagnosis was based upon by fungal culture in Sabouraud agar, which allowed to identify the dermatophyte species and,thus, the possible source of infection. Dermatophyte infections can be treated with systemic or topical antifungal medications. Because it was a young animal, it was chosen in the present case only for topical use, which proved to be adequate, probably due to the action of the drug in the areas of alopecia skin and the improvement of the systemic condition of theanimal. Knowing that the maned wolf is considered as a vulnerable species, it is important to know the diseases that affect this species, in order to carry out, when necessary, disease monitoring programs, preventive and therapies, which is essential for its preservation.Keywords: wild animal, disease, skin, dermatophyte.

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28

Yassine, Merad, Adjmi-Hamoudi Haiet, Tabet-Derraz Narjess, and Merad Fatima Zohra Samia. "Tinea Corporis Caused by Microsporum Canis in HIV Patient Treated For Neuromeningeal Cryptococcis: Report of A Nosocomial Outbreak." Journal of Current Medical Research and Opinion 1, no. 04 (July 25, 2018): 16–18. http://dx.doi.org/10.15520/jcmro.v1i04.54.

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We report a nosocomial outbreak of Microsporum canis during inpatient treatment for HIV newly diagnosed with cryptococcal meningitis. The clinical presentation, though very typical of ringworm infection, samples were collected and examined by direct microscopy and culture on Sabouraud’s dextrose agar, revealing Microsporum canis. It is strongly believed that outdoor cats living in the hospital spaces are the main cause of disease transmission. Measures to limit zoophilic pathogens are necessary in immunosuppressed inpatient care unit

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29

Kisera, Y. V., Y. V. Martyniv, and B. V. Gutyj. "Dynamics of morphological, immunological and histological changes in microsporіа in guinea pigs." Regulatory Mechanisms in Biosystems 12, no. 2 (May 22, 2021): 206–11. http://dx.doi.org/10.15421/022129.

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Microsporіа affect different species of animals and humans. The high contagiousness of the pathogen determines the relevance of research into this disease. Microsporum canis is the pathogen that most often causes microsporia. Weakened functions of the immune system and violation of the epithelial barrier of the skin are a favourable factor that causes microspores. The main source of infection is cats, which are involved in the storage and transmission of the pathogen. To clarify the dynamics of morphological, immunological and histological changes in microsporia, blood and skin studies of guinea pigs infected with M. canis were carried out. The animals were divided into two groups of 6 guinea pigs (healthy and sick). Test material (blood and skin) was taken from clinically healthy and sick animals 21 and 42 days after infection. The number of erythrocytes and leukocytes was determined by counting them in the Goryaev chamber, the hemoglobin content – by the method of cyanide hemoglobin. The leukogram was derived based on the counting and differentiation of 200 leukocyte cells in blood smears. Material for histological examination (pieces of skin) was fixed in 10–12% cooled solution of neutral formalin, followed by pouring in paraffin according to the scheme proposed by G. A. Merkulov. The obtained results demonstrated that leukocytosis developed in guinea pigs with microsporia on the 21st and 42nd days; the number of rod-shaped neutrophils increased, that of segmental neutrophils decreased, and that of ESR increased. The immune response to the course of microsporia was manifested in an increase in the percentage of T-lymphocytes, T-suppressors and a decrease in T-helper cells and an increase in T-killers compared with healthy animals. Histological examination showed that on the 21st day after infection, hyphae and spores of the fungus M. canis were localized in the skin. There is swelling of the dermis, stratification of collagen fibers and the accumulation of inflammatory infiltrates around the hair follicles. On the 42nd day, the infiltration spread and dystrophic changes in the skin occurred in the form of desquamation of the epidermis and the formation of acanthosis and hyperkeratosis on the surface of the dermis. The conducted research will allow further assessment of the course of microsporia under the action of various drugs and help establish the most effective method of treatment.

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30

Lestari, Rini. "ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis." Collaborative Medical Journal (CMJ) 3, no. 2 (July 9, 2020): 76–81. http://dx.doi.org/10.36341/cmj.v3i2.1302.

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Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

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Aneke, Chioma, Domenico Otranto, and Claudia Cafarchia. "Therapy and Antifungal Susceptibility Profile of Microsporum canis." Journal of Fungi 4, no. 3 (September 5, 2018): 107. http://dx.doi.org/10.3390/jof4030107.

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Microsporum canis is a worldwide diffused zoophilic dermatophyte which causes clinical conditions often characterised by multifocal alopecia, scaling, and circular lesions in many animal species, including humans. A large variety of oral and topical antifungal protocols is available for treating M. canis infection. However, the efficacy of these drugs and treatment protocols is variable, with treatment failure up to 40% of patients possibly due to resistance phenomena. The lack of standardised reference methods for evaluating the antifungal susceptibility of M. canis represents a major hindrance in assessing microbiological resistance in unresponsive clinical cases. Therefore, data about conventional therapy against M. canis and the protocols employed to test the antifungal activity of the most commonly employed drugs (i.e., azoles, polyenes, allylamines, and griseofulvin) have been summarised herein. This article focuses on technical parameters used for antifungal susceptibility tests, their effects on the minimum inhibitory concentration value, as well as their clinical implications.

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32

DOBROWOLSKA, ANITA, JOANNA DĘBSKA, MAGDALENA KOZŁOWSKA, and PAWEŁ STĄCZEK. "Strains Differentiation of Microsporum canis by RAPD Analysis Using (GACA)4 and (ACA)5 Primers." Polish Journal of Microbiology 60, no. 2 (2011): 145–48. http://dx.doi.org/10.33073/pjm-2011-020.

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Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T rubrum (CBS 120358), T mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.

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33

Bojanovsky, Anna. "Kleinepidemie durch Microsporum canis Bodin in Mannheim." Mycoses 20, no. 10 (April 24, 2009): 389–92. http://dx.doi.org/10.1111/j.1439-0507.1977.tb01483.x.

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34

Lana, Daiane, Paula Reginatto, William Lopes, Marilene Vainstein, and Alexandre Fuentefria. "Invasion of Human Nails by Microsporum canis." Journal of Infectiology 2, no. 4 (July 1, 2019): 36–38. http://dx.doi.org/10.29245/2689-9981/2019/4.1151.

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35

최용우, 권오성, 방용준, and 박준수. "Microscopic Findings of Macroconidia in Microsporum canis." Journal of Mycology and Infection 22, no. 2 (June 2017): 84–85. http://dx.doi.org/10.17966/kjmm.2017.22.2.84.

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36

Calles Monar, P. S., and A. Juárez Martín. "Tiña palpebral con blefaritis por Microsporum canis." Archivos de la Sociedad Española de Oftalmología 93, no. 10 (October 2018): 491–93. http://dx.doi.org/10.1016/j.oftal.2018.04.005.

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37

Romano, C., E. Paccagnini, and L. Pelliccia. "Case report. Onychomycosis due to Microsporum canis." Mycoses 44, no. 3-4 (April 2001): 119–20. http://dx.doi.org/10.1046/j.1439-0507.2001.00623.x.

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PIRACCINI, B. M., R. MORELLI, C. STINCHI, and A. TOSTI. "Proximal subungual onychomycosis due to Microsporum canis." British Journal of Dermatology 134, no. 1 (January 1996): 175–77. http://dx.doi.org/10.1111/j.1365-2133.1996.tb07863.x.

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PIRACCINI, B. M., R. MORELLI, C. STINCHI, and A. TOSTI. "Proximal subungual onychomycosis due to Microsporum canis." British Journal of Dermatology 134, no. 1 (January 1996): 175–77. http://dx.doi.org/10.1046/j.1365-2133.1996.d01-759.x.

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40

Hughes, J. R., and A. C. Pembroke. "Microsporum canis infection of the thumb-nail." Clinical and Experimental Dermatology 19, no. 3 (May 1994): 281. http://dx.doi.org/10.1111/j.1365-2230.1994.tb01193.x.

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Ernst, T. M. "Kerionartige Tinea barbae hervorgerufen durch Microsporum canis*." Mycoses 23, no. 1 (April 24, 2009): 35–37. http://dx.doi.org/10.1111/j.1439-0507.1980.tb02554.x.

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Dias, Tatiana, Orionalda de Fátima Lisboa Fernandes, Ailton José Soares, Xisto Sena Passos, Milce Costa, Lúcia Kioko Hasimoto e. Souza, and Maria do Rosário Rodrigues Silva. "Tinha do couro cabeludo em crianças de Goiânia, Brasil." Revista da Sociedade Brasileira de Medicina Tropical 36, no. 6 (December 2003): 653–55. http://dx.doi.org/10.1590/s0037-86822003000600002.

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Durante o período de janeiro de 1999 a julho de 2002 um total de 164 casos de tinha do couro cabeludo foram diagnosticados através de exames micológicos, realizados no Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás. Destes pacientes, 94 (57,3%) pertenciam ao sexo masculino, com idades variando de 3 meses a 13 anos. O diagnóstico e identificação dos agentes de dermatofitoses do couro cabeludo foram feitos utilizando-se exame direto com KOH a 20% e cultivo em ágar Mycobiotic e em ágar Sabouraud dextrose acrescido de cloranfenicol. As seguintes espécies foram identificadas: Microsporum canis (71,3%), Trichophyton tonsurans (11%), Trichophyton mentagrophytes (7,9%), Trichophyton rubrum (6,7%) and Microsporum gypseum (3%). Nossos estudos mostraram que o fungo de habitat natural no animal (zoofílico), Microsporum canis foi o agente mais comum de lesões no couro cabeludo em humanos.

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Abdel-Aziz, Marwa M., Mohsen S. Al-Omar, Hamdoon A. Mohammed, and Tamer M. Emam. "In Vitro and Ex Vivo Antibiofilm Activity of a Lipopeptide Biosurfactant Produced by the Entomopathogenic Beauveria bassiana Strain against Microsporum canis." Microorganisms 8, no. 2 (February 9, 2020): 232. http://dx.doi.org/10.3390/microorganisms8020232.

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Microsporum canis is one of the most important dermatophyte causing tinea corporis and tinea capitis and its biofilm-form has a poor therapeutic response. The biosurfactant production by entomopathogenic fungi (EPF) has not been reported yet. The study aimed to investigate the potential usage of the EPF biosurfactant in the eradication of an ex vivo biofilm of Microsporum canis (M. canis) for the first time. An entomopathogenic fungus was isolated from the fungal-infected Vespa orientalis wasp and identified as Beauveria bassiana (MN173375). Chemical characterization revealed the lipopeptide nature of the B. bassiana biosurfactant (BBLP). Efficient antifungal and antibiofilm activities of BBLP against M. canis in vitro were detected. An ex vivo hair model was used to investigate the efficiency of BBLP against M. canis biofilm, in a scenario close to the in vivo conditions. M. canis ex vivo biofilm eradication was confirmed in stereo, scanning electron, and fluorescent images. Also, the ex vivo biofilm was less susceptible to BBLP treatment compared to its in vitro counterpart. In conclusion, BBLP showed significant eradication to the M. canis ex vivo biofilm and open horizons to use bio-resource derived from EPF in controlling microbial biofilm and holding great promise for combating recalcitrant dermatophytosis.

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I Sakan, Gerson Yohanes, Soedarmanto Indarjulianto, Alfarisa Nururrozi, Yanuartono Yanuartono, and Slamet Raharjo. "SENSITIVITY OF Microsporum canis ISOLATED FROM DOGS TOWARDS ANTIFUNGALS." Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences 14, no. 4 (February 5, 2021): 108–11. http://dx.doi.org/10.21157/j.ked.hewan.v14i4.17617.

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The dermatophytosis treatment in dogs can fail due to dermatophyte resistance to antifungals. The purpose of this study is to determine the antifungal sensitivity of dermatophyte Microsporum canis (M. canis) isolated from dogs with dermatophyte. A total of 17 M. canis dog isolateswere tested for sensitivity to 25 µg fluconazole, 25 µg griseofulvin, 15 µg ketoconazole and 30 µg terbinafine, using the disc diffusion method.The M. canis isolates were cultured on Sabouraud's Dextrose Agar media, placed an antifungal disc, and then incubated at 28 C for 7 days. Thediameter of inhibition zone was measured, then compared with the standard, to determine whether they were sensitive, intermediate or resistant.The results of this study indicate that 16 isolates were sensitive to fluconazole; 17 isolates were sensitive to griseofulvin, ketoconazole andterbinafine; and one isolate was intermediate to fluconazole. Based on these results, it is concluded that all 17 (100%) isolates are sensitive to theantifungals griseofulvin, ketoconazole, terbinafine, and 16 (94%) isolates are sensitive to fluconazole. Thus, the four antifungals can be selectedto treat dermatophytes in dogs.

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Aste, Nicola, Anna Luisa Pinna, Monica Pau, and P. Biggio. "Kerion Celsi in a newborn due to Microsporum canis. Fallbericht. Durch Microsporum canis verursachtes Kerion Celsi bei einem Neugeborenen." Mycoses 47, no. 5-6 (June 2004): 236–37. http://dx.doi.org/10.1111/j.1439-0507.2004.00967.x.

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Aste, Nicola, and Monica Pau. "Tinea capitis caused by Microsporum canis treated with terbinafine. Behandlung von tinea capitis ausgeloest durch Microsporum canis mit Terbinafin." Mycoses 47, no. 9-10 (October 2004): 428–30. http://dx.doi.org/10.1111/j.1439-0507.2004.01024.x.

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Tabart, Jeremy, Aline Baldo, Sandy Vermout, Betty Nusgens, Charles Lapiere, Bertrand Losson, and Bernard Mignon. "Reconstructed interfollicular feline epidermis as a model for Microsporum canis dermatophytosis." Journal of Medical Microbiology 56, no. 7 (July 1, 2007): 971–75. http://dx.doi.org/10.1099/jmm.0.47115-0.

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Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant in vivo studies particularly difficult to perform. The aim of this study was to develop a new in vitro model of M. canis dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis, and to investigate its relevance in studying the host–pathogen relationship. Histological analysis of reconstructed interfollicular feline epidermis (RFE) revealed a fully differentiated epidermis. A proliferation assay showed replicating cells only in the basal layer, indicating that RFE is a well-stratified living tissue, leading to the formation of a horny layer. Histopathological analysis of RFE infected by M. canis arthroconidia revealed that the fungus invades the stratum corneum and produces SUB3, a keratinase implicated in the infectious process. In view of these results, an M. canis dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural M. canis feline infections.

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Mao, Liming, Liping Zhang, Hua Li, Wei Chen, Hongbin Wang, Shuxian Wu, Caiqin Guo, et al. "Pathogenic Fungus Microsporum canis Activates the NLRP3 Inflammasome." Infection and Immunity 82, no. 2 (December 9, 2013): 882–92. http://dx.doi.org/10.1128/iai.01097-13.

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ABSTRACTMicrosporum canisis a pathogenic fungus with worldwide distribution that causes tinea capitis in animals and humans.M. canisalso causes invasive infection in immunocompromised patients. To defy pathogenic fungal infection, the host innate immune system is the first line of defense. As an important arm of innate immunity, the inflammasomes are intracellular multiprotein complexes that control the activation of caspase-1, which cleaves proinflammatory cytokine pro-interleukin-1β (IL-1β) into its mature form. To determine whether the inflammasome is involved in the host defense againstM. canisinfection, we challenged human monocytic THP-1 cells and mouse dendritic cells with a clinical strain ofM. canisisolated from patients with tinea capitis. We found thatM. canisinfection triggered rapid secretion of IL-1β from both THP-1 cells and mouse dendritic cells. Moreover, by using gene-specific shRNA and competitive inhibitors, we determined thatM. canis-induced IL-1β secretion was dependent on NLRP3. The pathways proposed for NLRP3 inflammasome activation, namely, cathepsin B activity, K+efflux, and reactive oxygen species production, were all required for the inflammasome activation triggered byM. canis. Meanwhile, Syk, Dectin-1, and Card9 were found to be involved inM. canis-induced IL-1β secretion via regulation of pro-IL-1β transcription. More importantly, our data revealed thatM. canis-induced production of IL-1β was dependent on the NLRP3 inflammasomein vivo. Together, this study unveils that the NLRP3 inflammasome exerts a critical role in host innate immune responses againstM. canisinfection, and our data suggest that diseases that result fromM. canisinfection might be controlled by regulating the activation of inflammasomes.

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Stuntebeck, Rebecca, Karen A. Moriello, and Maria Verbrugge. "Evaluation of incubation time for Microsporum canis dermatophyte cultures." Journal of Feline Medicine and Surgery 20, no. 10 (September 18, 2017): 997–1000. http://dx.doi.org/10.1177/1098612x17729286.

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Objectives The goal of this study was to determine how frequently Microsporum canis was isolated after 1, 2 and 3 weeks of incubation on dermatophyte culture medium either from untreated cats or cats during treatment. Methods This was an observational retrospective study. Toothbrush fungal culture results were examined from two data pools: untreated cats with suspect skin lesions and weekly fungal cultures from cats being treated for dermatophytosis. Results Results from 13,772 fungal cultures were reviewed and 2876 (20.9%) were positive for M canis. Of these, 2800 were confirmed as positive within 14 days of incubation and only 76 (2.6%) required >14 days for confirmation of M canis. In pretreatment specimens, 98.2% (1057/1076) of M canis isolates were recovered within 14 days of incubation in specimens from cats not known to have received prior antifungal treatment. For cats receiving treatment, 96.8% (1743/1800) of M canis isolates were recovered within 14 days of incubation. Of the 57 cultures that required >14 days for finalization, 21 required extra incubation time because cultures were grossly abnormal, 12 had concurrent contaminant growth delaying microscopic confirmation and 24 had no growth in the first 14 days. Of these 24, 19 had 1–2 colony-forming units (cfu)/plate and the remaining five plates had 5 to >10 cfu/plate, all with abnormal morphology. Conclusions and relevance The findings of this study show that it is not necessary to hold pretreatment or post-treatment fungal cultures for 21 days before finalizing cultures for no growth. Growth requiring >14 days had grossly abnormal morphology.

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Díaz, Melissa Gabriela, Liz Soledad Sanabria, Gustavo Aguilar, Patricia Araujo, José Pereira, and José Félix Plans. "Isolation of Microsporum canis and Microsporum gypseum in asymptomatic cats from the metropolitan area of Asunción-Paraguay." DEL NACIONAL 9, no. 2 (December 30, 2017): 12–19. http://dx.doi.org/10.18004/rdn2017.0009.02.012-019.

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