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Journal articles on the topic 'Microtechnique'

Author: Grafiati

Published: 4 June 2021

Last updated: 30 January 2023

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1

Ermayanti, E., Yenny Anwar, and Didi Jaya Santri. "Analysis of students’ creative thinking on plant microtechnical laboratory practices." JPBI (Jurnal Pendidikan Biologi Indonesia) 7, no. 2 (July 27, 2021): 111–16. http://dx.doi.org/10.22219/jpbi.v7i2.12321.

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Empowerment of creative thinking skills is important in every lecture activity, including practicum. This study aimed to analyze students' creative thinking skills in Plant Microtechnique practicum. This research used descriptive method in which the participants were the seventh semester biology education students (n=20) of State University of South Sumatera, Indonesia. The student has taken Plant Microtechnique Course in the previous semester. The instrument used was essay test developed based on creative thinking indicator. The data were processed by calculating the percentage for each indicator and categorized into three levels (i.e., low, medium, and high). The findings revealed that students’ creative thinking skills in Plant Microtechnique laboratory practices were medium for fluency indicator, and low for flexibility, originality, and elaboration indicators. Therefore, it is necessary to revise learning strategies that support the empowerment of students' creative thinking in Microtechnique Laboratory practices.

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2

Schmid, Rudolf, and Steven E. Ruzin. "Plant Microtechnique and Microscopy." Taxon 48, no. 3 (August 1999): 619. http://dx.doi.org/10.2307/1224595.

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3

Wernicke, W. "Plant Microtechnique and Microscopy." Micron 32, no. 4 (June 2001): 455–56. http://dx.doi.org/10.1016/s0968-4328(00)00007-x.

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4

Schichnes, Denise, Jeff Nemson, Lorraine Sohlberg, and Steven E. Ruzin. "Microwave Protocols for Paraffin Microtechnique and In Situ Localization in Plants." Microscopy and Microanalysis 4, no. 5 (October 1998): 491–96. http://dx.doi.org/10.1017/s1431927698980461.

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We have developed a microwave protocol for a paraffin-embedding microtechnique of the shoot apical meristem of Zea mays and have successfully applied this protocol to other plant tissues. This protocol decreases the time required for all aspects of microtechnique tissue processing, including fixation (24 hr to 15 min), dehydration (73 hr to 10 min), and infiltration (96 hr to 3 hr). Additionally, the time required to adhere paraffin ribbons to gelatin-coated slides and for the Johanson's safranin O, fast green FCF staining protocol has been significantly decreased. Using this technique, the quality of tissue preservation and subsequent in situ localization of knotted mRNA was increased by using microwaves.

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5

Pérez-Morga, David L., and Paul T. Englund. "Microtechnique for electron microscopy of DNA." Nucleic Acids Research 21, no. 5 (1993): 1327–28. http://dx.doi.org/10.1093/nar/21.5.1327.

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6

Bojko, Barbara, Fatemeh Mirnaghi, and Janusz Pawliszyn. "Solid-phase microextraction: a multi-purpose microtechnique." Bioanalysis 3, no. 17 (September 2011): 1895–99. http://dx.doi.org/10.4155/bio.11.210.

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7

Krikorian, Abraham D. "Plant Microtechnique and Microscopy. Steven E. Ruzin." Quarterly Review of Biology 75, no. 2 (June 2000): 188–89. http://dx.doi.org/10.1086/393420.

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8

Poulsen, Knud. "MICROTECHNIQUE FOR QUANTITATIVE MEASUREMENT OF RENIN IN PLASMA." Acta Pathologica Microbiologica Scandinavica 69, no. 1 (August 15, 2009): 19–27. http://dx.doi.org/10.1111/j.1699-0463.1967.tb05122.x.

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9

Tseng, K.-F., W. Chen, and D. Zhang. "Dissecting Contractile Ring Assembly Using a Multimode Microtechnique." Microscopy and Microanalysis 16, S2 (July 2010): 996–97. http://dx.doi.org/10.1017/s1431927610060769.

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10

Schichnes, Denise, Jeffrey A. Nemson, and Steven E. Ruzin. "Microwave Protocols for Plant and Animal Paraffin Microtechnique." Microscopy Today 13, no. 3 (May 2005): 50–53. http://dx.doi.org/10.1017/s1551929500051658.

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The microwave oven is a valuable tool for light and electron microscopy microtechnique labs. Tissue processing times, traditionally taking up to two weeks, have been reduced to a few hours as a result of the implementation of microwave technology (Kok et al., 1988, Gibberson and Demaree, 2001). In addition, the quality of the tissue preparations has improved dramatically. Microwave ovens have also evolved since their first use in the laboratory. Early experiments were conducted using relatively crude commercial microwave ovens. Now, labs use microwave ovens with temperature probes, strict control over the magnetron (which generates the microwaves), variable power supplies, chamber cooling, and high microwave field uniformity.

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11

Schwartz, Kenneth A., John A. Gauger, and John M. Davis. "Precise quantitation of palgg: A new radiometric microtechnique." American Journal of Hematology 33, no. 3 (March 1990): 167–76. http://dx.doi.org/10.1002/ajh.2830330304.

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12

MENEZES, Carla M. S., Karin KIRCHGATTER, Sílvia M. F. DI SANTI, Carine SAVALLI, Fabíola G. MONTEIRO, Gilberto A. PAULA, and Elizabeth I. FERREIRA. "In vitro evaluation of erythromycin in chloroquine resistant brazilian P. falciparum freshly isolates: modulating effect and antimalarial activity evidence." Revista do Instituto de Medicina Tropical de São Paulo 41, no. 4 (July 1999): 249–53. http://dx.doi.org/10.1590/s0036-46651999000400009.

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Erythromycin, a reversal agent in multidrug-resistant cancer, was assayed in chloroquine resistance modulation. The in vitro microtechnique for drug susceptibility was employed using two freshly isolates of Plasmodium falciparum from North of Brazil. The antimalarial effect of the drug was confirmed, with an IC50 estimates near the usual antimicrobial therapy concentration, and a significant statistical modulating action was observed for one isolate.

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13

Kiernan, J. A. "Dyes and other colorants in microtechnique and biomedical research." Coloration Technology 122, no. 1 (February 2006): 1–21. http://dx.doi.org/10.1111/j.1478-4408.2006.00009.x.

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14

Williams, Mike A. "Biological microtechnique (microscopy handbook 28 of royal microscopial society)." Trends in Cell Biology 5, no. 6 (June 1995): 257. http://dx.doi.org/10.1016/s0962-8924(00)89027-3.

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15

Miller, Bernard, and Ilya Tyomkin. "A microtechnique for determining the surface tension of a liquid." Journal of Adhesion Science and Technology 6, no. 12 (January 1992): 1371–79. http://dx.doi.org/10.1163/156856192x00683.

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16

Abiev, R. Sh. "Modern state and perspectives of microtechnique application in chemical industry." Russian Journal of General Chemistry 82, no. 12 (December 2012): 2019–24. http://dx.doi.org/10.1134/s1070363212120237.

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17

Yang, Shen Lin, Silvia Maria Di Santi, Vicente Amato Neto, Antonio Augusto Baillot Moreira, Pedro Luiz Silva Pinto, Marcos Boulos, Rubens Campos, Eunice José de Sant'Ana, and Mario Shiroma. ""In vitro" activity of gentian violet against asexual Plasmodium falciparum parasites." Revista do Instituto de Medicina Tropical de São Paulo 30, no. 1 (February 1988): 17–20. http://dx.doi.org/10.1590/s0036-46651988000100003.

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In order to investigate whether gentian violet exhibited "in vitro" inhibitory activity against Plasmodium falciparum, the Authors have carried out 20 sensitivity tests according to the microtechnique described by RIECK MANN et al.5. Results have shown inhibition of schizonts'maturation at the following concentration: 1/1000; 1/1500; 1/2000; 1/2500; 1/3000 and 1/4000, thus demonstrating inhibitory activity of the tested dye against asexual blood parasites. The present data suggest gentain violet may be possibly used in the prophylaxis of transfusion-acquired malaria.

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18

Harrison, L. M., J. E. Morrison, and P. V. Fennessey. "Microtechnique for quantifying phenol in plasma by gas chromatography-mass spectrometry." Clinical Chemistry 37, no. 10 (October 1, 1991): 1739–42. http://dx.doi.org/10.1093/clinchem/37.10.1739.

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Abstract Methods for detection and quantification of phenol have been developed primarily for use in environmental and industrial monitoring, given the widespread use of phenol as a disinfectant and antiseptic. Little information is available regarding concentrations of phenol in the blood of patients treated with phenol in regional nerve blocks (e.g., intrathecal) for temporary relief of pain or spasticity. We report a specific and sensitive method for quantifying phenol in plasma, using chemical derivatization and high-resolution capillary column gas chromatography in conjunction with mass spectrometry. The assay we describe was developed to monitor plasma concentrations of phenol in children given motor point nerve blocks with dilute phenol.

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19

Degen, I. A. "A Simple Microtechnique for Obtaining Pyrolyzate Infrared Spectra from Polymeric Materials." Applied Spectroscopy 44, no. 9 (November 1990): 1587–88. http://dx.doi.org/10.1366/0003702904417805.

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20

Overall, Christopher M. "A microtechnique for dialysis of small volume solutions with quantitative recoveries." Analytical Biochemistry 165, no. 1 (August 1987): 208–14. http://dx.doi.org/10.1016/0003-2697(87)90221-1.

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21

LUO, Zai. "Effect of Form Factors on Thermal Deformation of Mechanical Parts in Microtechnique." Chinese Journal of Mechanical Engineering 45, no. 01 (2009): 235. http://dx.doi.org/10.3901/jme.2009.01.235.

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22

Susilawati, Puspita Ratna. "The Implementation of Flipped Learning in Squash Method Material in Microtechnique Course." BIOEDUSCIENCE 4, no. 2 (December 31, 2020): 166–76. http://dx.doi.org/10.22236/j.bes/424825.

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Background: The implementation of flipped learning could be one of the solutions offered so that practicum that was limit by time could be carried out. This study aimed to determine the effect of implementing the flipped learning model on student understanding of the squash method material in microtechnique courses. Methods: This quasi study used a non-equivalent control group design. In the treatment group the flipped learning model was applied and an analysis of its effect on student understanding was carried out. The research data were obtained through the pretest and posttest. The pretest is used to evaluate the ability to remembering and understanding, while the posttest evaluates the ability to analyze, evaluate and create. The pretest and posttest value data were used to calculate the N-gain value, then the Mann-Whitney U test was performed to determine the difference between the two. Results: The increase in the mean value in the treatment class was higher than in the control class. The treatment class was 5.2, while the control class was 0.82. The percentage of students who showed a high and moderate N-gain score in the treatment class was 54.05%, while the control class was 40%. There was no difference between the N-gain value in the treatment and control classes. The flipped learning model's implementation did not affect student understanding but had been able to increase student understanding of the squash method material. Conclusions: The flipped learning model could be applied as a solution to practical problems constrained by time constraints.

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23

Kestin, Kathryn J., and Paul V. Fennessey. "Microtechnique for Quantitation of Plasma Methohexital Using Gas Chromatography and Mass Spectrometry." Anesthesia & Analgesia 67, no. 5 (May 1988): 466???468. http://dx.doi.org/10.1213/00000539-198805000-00009.

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24

Kiernan, John A. "Double Embedding in Cellulose Nitrate and Paraffin Wax, an Old and Useful Method that is Easily Misunderstood." Microscopy Today 6, no. 2 (March 1998): 12–15. http://dx.doi.org/10.1017/s1551929500059563.

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Cellulose nitrate is also known as celloidin, collodion, low viscosity nitrocellulose (LVN) necoloidine, nitrocellulose, parlodion, pyroxylin, and soluble gun cotton. (Some of these are trade-names; for even more synonyms, see the Merck Index, under pyroxylin.) It is made by treating cellulose with a mixture of concentrated nitric and sulfuric acids, This converts some of the hydroxyl (-OH) groups of cellulose to nitrate ester (-ONO2) groups. The molecular size and physical form of the original cellulose and the proportion of nitrated hydroxyl groups determine the properties of the product. It is useful in microtechnique because of its physical properties and its unusual responses to solvents and other liquids.

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25

Menezes, Carla M. S., Karin Kirchgatter, Sílvia M. Di Santi, Carine Savalli, Fabíola G. Monteiro, Gilberto A. Paula, and Elizabeth I. Ferreira. "In vitro evaluation of verapamil and other modulating agents in Brazilian chloroquine-resistant Plasmodium falciparum isolates." Revista da Sociedade Brasileira de Medicina Tropical 36, no. 1 (January 2003): 5–9. http://dx.doi.org/10.1590/s0037-86822003000100002.

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Verapamil, was assayed to record its modulating effect upon Brazilian Plasmodium falciparum isolates resistant to chloroquine. Other cardiovascular drugs known to be modulating agents in resistant malaria and/or multidrug-resistant neoplasias, including nifedipine, nitrendipine, diltiazem and propranolol, were also evaluated. Concentrations similar to those for cardiovascular therapy were used in the in vitro microtechnique for antimalarial drug susceptibility. Intrinsic antiplasmodial activity was observed from the lowest concentrations without a significant modulating action. Other reported modulating agents, such as the antipsychotic drug trifluoperazine and the antidepressants desipramine and imipramine, demonstrated similar responses under the same experimental conditions. Results suggest a much higher susceptibility of Brazilian strains, as well as an indifferent behaviour in relation to modulating agents.

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26

Ufodike, E. B. C., J. De La Noue, D. Proulx, and T. O. Ojobe. "A Modified Microtechnique for Estimating Protein and Amino Acid Digestibility in Fish Fry." Progressive Fish-Culturist 57, no. 3 (July 1995): 238–41. http://dx.doi.org/10.1577/1548-8640(1995)057<0238:ammfep>2.3.co;2.

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27

CHICK, ANDREW I. R. "Stains for entomological microtechnique: simple stains for whole mounts and dissection." Zootaxa 4790, no. 3 (June 12, 2020): 447–72. http://dx.doi.org/10.11646/zootaxa.4790.3.2.

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Slide mounted entomological specimens often require the aid of contrast techniques to improve the clarity of morphological characteristics. Methods can involve the use of techniques such as Phase contrast, Dark field or differential interference contrast microscopy (DIC), however where an entomologist may only have access to simple brightfield microscopy chemical staining of the specimen may be used to improve contrast. For whole mounts of entomological specimens, a single stain, occasionally two, are often used, in comparison to histological sections that often employ multiple stains in complex protocols. A number of authors have proposed different stains and staining methods for a number of insect groups with few considering the long term qualities of the stain, it has previously been shown that aniline dyes are prone to fading in Canada Balsam mounts, and that some stains fade even when protected from sunlight. This paper aims to summarise the knowledge of stains used for entomological specimens and provide details on the archival qualities.

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28

Wagih, Elsayed E., and Jacqueline Fletcher. "Zymoblot, a new microtechnique used to detect enzyme activity in spiroplasmas and bacteria." Canadian Journal of Microbiology 39, no. 5 (May 1, 1993): 543–47. http://dx.doi.org/10.1139/m93-077.

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A new microtechnique that detects enzyme activity in prokaryotes is described. The technique, designated zymoblot, is based on the immobilization of negatively charged enzymes from an alkaline extract spotted onto a nitrocellulose membrane. The presence of specific enzyme activity in the extract is selectively assayed with a reaction mixture containing the corresponding substrate. The enzyme–substrate reaction produces an insoluble colored product that accumulates at the site. The zymoblot technique offers the advantages of simplicity, sensitivity, reproducibility, speed, and the use of microquantities of reactants. The protein in the spot can be visualized by a technique termed "proteinblot," in which the protein is stained with Coomassie blue. Esterase and tyrosinase were detected by the zymoblot method in six spiroplasmas including four strains of Spiroplasma citri, one of Spiroplasma kunkelii, and one of Spiroplasma melliferum, and two bacteria, Pseudomonas syringae pv. syringae B301D and Escherichia coli K-12. Acid phosphatase, alkaline phosphatase, alanine dehydrogenase, peroxidase, and 6-phosphogluconate dehydrogenase were not detected in any of the spiroplasmas, but were each detected in one or both of the walled bacteria.Key words: spiroplasma, enzyme, protein, zymoblot.

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29

Kohashi, Osamu, Yukiko Kohashi, Shizue Toyoshima, and Nobuaki Shigematsu. "A simple and rapid microtechnique for studying a luminol-dependent chemiluminescence of whole blood." Ensho 5, no. 3 (1985): 205–10. http://dx.doi.org/10.2492/jsir1981.5.205.

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30

Iwu, Maurice, Joan Jackson, John Tally, and Daniel Klayman. "Evaluation of Plant Extracts for Antileishmanial Activity using a Mechanism-Based Radiorespirometric Microtechnique (RAM)." Planta Medica 58, no. 05 (October 1992): 436–41. http://dx.doi.org/10.1055/s-2006-961508.

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31

Handayani, Rd Selvy, Muhamad Yusuf, and Ajmir Akmal. "Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine." Journal of Tropical Horticulture 1, no. 1 (November 9, 2018): 10. http://dx.doi.org/10.33089/jthort.v1i1.6.

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The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.

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32

Muniz-Junqueira, Maria Imaculada, Lídia Maria Figueira Peçanha, Valeriano Luiz da Silva-Filho, Maria Cecília de Almeida Cardoso, and Carlos Eduardo Tosta. "Novel Microtechnique for Assessment of Postnatal Maturation of the Phagocytic Function of Neutrophils and Monocytes." Clinical Diagnostic Laboratory Immunology 10, no. 6 (November 2003): 1096–102. http://dx.doi.org/10.1128/cdli.10.6.1096-1102.2003.

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ABSTRACT We describe a simple test for the evaluation of phagocytosis and provide a chart of reference values to evaluate normal phagocytosis by age. We assessed the postnatal maturation of phagocytic function of neutrophils and monocytes. Phagocytosis was evaluated in newborn children delivered vaginally or by cesarean section, infants, preschool children, schoolchildren, and adult subjects. Two drops of blood were placed on a microscope slide and incubated with Saccharomyces cerevisiae yeasts, and phagocytosis was evaluated by microscopy. Our technique showed results comparable to or better than those obtained by other usual techniques. The neutrophils of newborn children delivered by cesarean section showed a phagocytic capacity 45% higher than those of neonates delivered vaginally, whereas neutrophils from children in the latter group showed the lowest phagocytic capacity of all age groups. Phagocytosis by neutrophils reached the levels seen in adults at about the first year of life, while there were no important variations in phagocytosis by monocytes in the different age groups. The technique described is reliable and fast, uses only a few drops of blood, and allows better preservation of cell function due to the minimal manipulation to which the cells are submitted. The delayed maturation of the phagocytic function by neutrophils may account for the high levels of susceptibility of newborn and infant children to bacterial infections. This practical method of assessment of phagocytosis may allow the diagnosis of primary or secondary phagocytic deficiencies to be made more easily and may allow better monitoring and treatment of those with dysfunctions of these cells.

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33

Hoon, A. H., C. K. Lam, and M. J. Wah. "Quantitative assessment of antimalarial activities from Malaysian Plasmodium falciparum isolates by modified in vitro microtechnique." Antimicrobial Agents and Chemotherapy 39, no. 3 (March 1, 1995): 626–28. http://dx.doi.org/10.1128/aac.39.3.626.

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34

Lazarus, Alan H., and Malcolm G. Baines. "A rapid and efficient microtechnique for the analysis of functional transferrin receptors on tumor cells." Journal of Immunological Methods 79, no. 2 (May 1985): 213–21. http://dx.doi.org/10.1016/0022-1759(85)90101-2.

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35

Frankenburg, Shoshana. "A simplified microtechnique for measuring human lymphocyte proliferation after stimulation with mitogen and specific antigen." Journal of Immunological Methods 112, no. 2 (September 1988): 177–82. http://dx.doi.org/10.1016/0022-1759(88)90355-9.

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36

Duclos, Ph, J. Freney, J. Caillet, H. Alexandre, and D. Garonnat. "Carbon source assimilation and fermentation tests: Study of 77 animal Pasteurella strains by a microtechnique." Comparative Immunology, Microbiology and Infectious Diseases 9, no. 1 (January 1986): 47–51. http://dx.doi.org/10.1016/0147-9571(86)90074-3.

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37

Burchell, Ann, Robert Hume, and Brian Burchell. "A new microtechnique for the analysis of the human hepatic microsomal glucose-6-phosphatase system." Clinica Chimica Acta 173, no. 2 (April 1988): 183–91. http://dx.doi.org/10.1016/0009-8981(88)90256-2.

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38

Mardiyyaningsih, A. N., and E. Daningsih. "Preparation of leaf anatomy slide using modification protocols." IOP Conference Series: Earth and Environmental Science 976, no. 1 (February 1, 2022): 012061. http://dx.doi.org/10.1088/1755-1315/976/1/012061.

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Abstract Preparing leaf anatomy slide is a routine procedure in physiology and anatomy plant research. A standard microtechnique method frequently adopted for doing such procedure is Johansens’. However, for early user such as university students, executing this method may be challenging in term of using many chemicals in different stages which make it more pricey and need longer duration. This research attempts to analyse the possibility for using simpler protocol suggested by Gunarso to replace Johansens method. It is conducted under a qualitative experiment design involving leaves from monocotyl and dicotyl classes as samples. Samples are analysed with safranin-fast green staining protocols. Result shows that there is no difference between Johanssens’ and Gunarso modified protocol (GMP) with respect to vivid observable of the cell membrane, the tissue structure, and the colour intensity. Hence, this new modification protocol is potential to replace the Johansens methods in leaf anatomy slide

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39

Mattila, Tuna, Jouko Syväjärvi, Niels-Einar Jensen, and Markus Sandholm. "Determinants of bacterial replication rates in mastitic whey." Journal of Dairy Research 53, no. 2 (May 1986): 197–202. http://dx.doi.org/10.1017/s0022029900024791.

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SUMMARYBacterial growth was measured by a turbidimetric microtechnique in the whey of milk samples from quarters of cows with subclinical mastitis. Samples were grouped according to bacterial isolates recovered and the effects of bacterial species and whey on bacterial growth rates were analysed. Different strains of bacteria and different whey samples gave highly significant differences in bacterial replication rates. Except for penicillin-resistant Staphylococcus aureus, bacteria grew better in whey from mastitic milk where the inflammation was caused by the same bacterial species than in other mastitic milk samples. Inflammation caused by major pathogens generally enhanced the growth in whey of any type of major pathogen. Since mastitis pathogens showed enhanced growth in whey prepared from the same milk from which they were isolated, specific antibacterial factors in the whey did not appear to restrict bacterial growth in whey. The nutritional quality of the medium seems to be the important determinant of bacterial growth.

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40

Edwards, C. A., P. A. Finger, D. J. Anderson, J. A. Wiler, J. F. Hetke, and R. A. Altschuler. "A Technique for In Vivo Morphological Evaluation of Chronically Implanted Neuronal Silicon Substrate Electrodes for Confocal Microscopy." Microscopy and Microanalysis 3, S2 (August 1997): 351–52. http://dx.doi.org/10.1017/s1431927600008643.

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Various multichannel silicon electrodes have been developed for stimulating and recording in the central nervous system of experimental animals. These electrodes can be used in their planar form or can be assembled into 3-D structures for volume interaction with tissue. However, bio-compatibility issues arise concerning the introduction of any electrode into living tissue. From earlier observations, we noticed that the neuronal cells had a tendency to adhere to the chronically implanted silicon substrate. Routine microtechnique became an obstacle when attempting to section the implanted and embedded electrode. Most often, this was attempted by passing a stainless steel blade through paraffin, or plastic embedded tissue, resulting in disrupting the tissue material interface. We needed to develop a reproducible procedure which preserved the integrity of this interface for future LM and confocal microscopic studies.In order to preserve the electrode/tissue interface, we used the Exakt microgrinding/micropolishing technique, where the electrode/brain block was embedded in Technovit 7200 resin and cut with a diamond impregnated band saw.

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41

Sheth, Kirtikant V., Mohammed Abdulatiff, and Sultan Al-Sedairy. "Modified Whole Blood Microtechnique for Measurement of Mitogens and Allogeneic Lymphocyte Stimulated Proliferation of Human Lymphocytes." Annals of Saudi Medicine 13, no. 6 (November 1993): 547–50. http://dx.doi.org/10.5144/0256-4947.1993.547.

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42

Johnson, D. R., and E. L. Kaplan. "Microtechnique for serum opacity factor characterization of group A streptococci adaptable to the use of human sera." Journal of Clinical Microbiology 26, no. 10 (1988): 2025–30. http://dx.doi.org/10.1128/jcm.26.10.2025-2030.1988.

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43

Brandizzi, F. "Ruzin SE. 1999. Plant microtechnique and microscopy. 322 pp. Oxford, New York: Oxford University Press. £32.50 (softback)." Annals of Botany 86, no. 3 (September 2000): 708. http://dx.doi.org/10.1006/anbo.2000.1231.

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44

Umeki, Shigenobu, and Dorothy M. DeLisle. "A microtechnique for neutrophil respiratory burst oxidase in a cell-free system—characterization of oxidase activation system." Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 96, no. 3 (January 1990): 461–64. http://dx.doi.org/10.1016/0305-0491(90)90040-z.

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45

Permana, Tutut Indria, and Sri Wahyuni. "Project-Based Learning: A Hands-On Activity to Improve Students’ Scientific Writing Skills through Lesson Study in Microtechnique Course." Journal of Biology Education 8, no. 3 (December 15, 2019): 340–47. http://dx.doi.org/10.15294/jbe.v8i3.33938.

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Project-based learning has been recognized as a dynamic classroom approach in which students actively explore real-world problems to obtain deeper knowledge. This study aimed to describe the implementation of project-based learning to improve students’ scientific writing skills. This action research study conducted based on Lesson Study (LS) which incorporated plan-do-see phase. The descriptive research was using 40 undergraduate students in the sixth semester in Biology Education Department, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang who attended Microtechnique course. The students’ scientific writing skills were measured using student worksheet which asked them to develop a scientific article. The measured parameters were how student proposed introduction, problem statement, method, result, discussion, conclusion, and reference. The data were analyzed descriptively. The result showed that students’ scientific writing skills were improved after implementing project-based learning. Some of the obstacles to implementing LS were related to the schedule and preparation. It can be resolved by conducting more intense coordination and regular discussions involving all research members.

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MENEZES, Carla M. S., Karin KIRCHGATTER, Sílvia Maria DI SANTI, Gilberto A. PAULA, and Elizabeth I. FERREIRA. "In vitro evaluation of quinidine sensitivity in brazilian Plasmodium falciparum isolates: comparative analysis to quinine and chloroquine." Revista do Instituto de Medicina Tropical de São Paulo 43, no. 4 (August 2001): 221–26. http://dx.doi.org/10.1590/s0036-46652001000400009.

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Falciparum malaria represents a serious and an increasing world public health problem due to the acquired parasite's resistance to the most available drugs. In some endemic areas, quinidine, a diastereoisomer of the antimalarial quinine, has been employed for replacing the latter. In order to evaluate the use of quinidine as an alternative to the increasing loss of quinine effectiveness in Brazilian P. falciparum strains, as has been observed in the Amazon area, we have assayed quinidine, quinine and chloroquine. The in vitro microtechnique was employed. All isolates showed to be highly resistant to chloroquine. Resistance to quinine was not noted although high MIC (minimal inhibitory concentration) values have been observed. These data corroborate the decreasing sensitivity to quinine in strains from Brazil. Quinidine showed IC50 from 0.053 to 4.577 mumol/L of blood while IC50 from 0.053 to 8.132 mumol/L of blood was estimated for quinine. Moreover, clearance of the parasitemia was observed in concentrations lower than that used for quinidine in antiarrhythmic therapy, confirming our previous data. The results were similar to African isolate.

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Ang, H. H., K. L. Chan, and J. W. Mak. "Susceptibility Studies of Plasmodium falciparum Isolates and Clones against Cycloguanil and Pyrimethamine Using the Modified In vitro Microtechnique." Journal of Parasitology 82, no. 6 (December 1996): 1029. http://dx.doi.org/10.2307/3284218.

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Parnet, F. "Expérience de mise en place du groupage et du phénotypage par microtechnique dans un laboratoire de surveillance prénatale." Revue Française de Transfusion et d'Hémobiologie 33, no. 2 (March 1990): 134–36. http://dx.doi.org/10.1016/s1140-4639(05)80016-4.

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Ahmad, Ateeq, Richard K. Gordon, and Peter K. Chiang. "A microtechnique for quantification of detergent-solubilized muscarinic and nicotinic acetylcholine receptors using a semi-automated cell harvester." FEBS Letters 214, no. 2 (April 20, 1987): 285–90. http://dx.doi.org/10.1016/0014-5793(87)80071-6.

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Gattu, Mahanandeeshwar, Alvin V. Terry, and Jerry J. Buccafusco. "A rapid microtechnique for the estimation of muscarinic and nicotinic receptor binding parameters using 96-well filtration plates." Journal of Neuroscience Methods 63, no. 1-2 (December 1995): 121–25. http://dx.doi.org/10.1016/0165-0270(95)00100-x.

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