Academic literature on the topic 'Microvascular constructs'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Microvascular constructs.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Microvascular constructs"

1

Sukmana, Irza. "Microvascular Guidance: A Challenge to Support the Development of Vascularised Tissue Engineering Construct." Scientific World Journal 2012 (2012): 1–10. http://dx.doi.org/10.1100/2012/201352.

Full text
Abstract:
The guidance of endothelial cell organization into a capillary network has been a long-standing challenge in tissue engineering. Some research efforts have been made to develop methods to promote capillary networks inside engineered tissue constructs. Capillary and vascular networks that would mimic blood microvessel function can be used to subsequently facilitate oxygen and nutrient transfer as well as waste removal. Vascularization of engineering tissue construct is one of the most favorable strategies to overpass nutrient and oxygen supply limitation, which is often the major hurdle in developing thick and complex tissue and artificial organ. This paper addresses recent advances and future challenges in developing three-dimensional culture systems to promote tissue construct vascularization allowing mimicking blood microvessel development and function encounteredin vivo. Bioreactors systems that have been used to create fully vascularized functional tissue constructs will also be outlined.
APA, Harvard, Vancouver, ISO, and other styles
2

Fan, Rong, Yihang Sun, and Jiandi Wan. "Leaf-inspired artificial microvascular networks (LIAMN) for three-dimensional cell culture." RSC Advances 5, no. 110 (2015): 90596–601. http://dx.doi.org/10.1039/c5ra20265e.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Vajda, Jernej, Marko Milojević, Uroš Maver, and Boštjan Vihar. "Microvascular Tissue Engineering—A Review." Biomedicines 9, no. 6 (2021): 589. http://dx.doi.org/10.3390/biomedicines9060589.

Full text
Abstract:
Tissue engineering and regenerative medicine have come a long way in recent decades, but the lack of functioning vasculature is still a major obstacle preventing the development of thicker, physiologically relevant tissue constructs. A large part of this obstacle lies in the development of the vessels on a microscale—the microvasculature—that are crucial for oxygen and nutrient delivery. In this review, we present the state of the art in the field of microvascular tissue engineering and demonstrate the challenges for future research in various sections of the field. Finally, we illustrate the potential strategies for addressing some of those challenges.
APA, Harvard, Vancouver, ISO, and other styles
4

LeBlanc, Amanda J., Jeremy S. Touroo, James B. Hoying, and Stuart K. Williams. "Adipose stromal vascular fraction cell construct sustains coronary microvascular function after acute myocardial infarction." American Journal of Physiology-Heart and Circulatory Physiology 302, no. 4 (2012): H973—H982. http://dx.doi.org/10.1152/ajpheart.00735.2011.

Full text
Abstract:
A three-dimensional tissue construct was created using adipose-derived stromal vascular fraction (SVF) cells and evaluated as a microvascular protection treatment in a myocardial infarction (MI) model. This study evaluated coronary blood flow (BF) and global left ventricular function after MI with and without the SVF construct. Fischer-344 rats were separated into four groups: sham operation (sham), MI, MI Vicryl patch (no cells), and MI SVF construct (MI SVF). SVF cells were labeled with green fluorescent protein (GFP). Immediately postinfarct, constructs were implanted onto the epicardium at the site of ischemia. Four weeks postsurgery, the coronary BF reserve was significantly decreased by 67% in the MI group and 75% in the MI Vicryl group compared with the sham group. The coronary BF reserve of the sham and MI SVF groups in the area at risk was not significantly different (sham group: 83 ± 22% and MI SVF group: 57 ± 22%). Griffonia simplicifolia I and GFP-positive SVF immunostaining revealed engrafted SVF cells around microvessels in the infarct region 4 wk postimplant. Overall heart function, specifically ejection fraction, was significantly greater in MI SVF hearts compared with MI and MI Vicryl hearts (MI SVF: 66 ± 4%, MI: 37 ± 8%, and MI Vicryl: 29 ± 6%). In conclusion, adipose-derived SVF cells can be used to construct a novel therapeutic modality for treating microvascular instability and ischemia through implantation on the epicardial surface of the heart. The SVF construct implanted immediately after MI not only maintains heart function but also sustains microvascular perfusion and function in the infarct area by sustaining the coronary BF reserve.
APA, Harvard, Vancouver, ISO, and other styles
5

Pedersen, Torbjorn O., Anna L. Blois, Zhe Xing, et al. "Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs." Stem Cell Research & Therapy 4, no. 3 (2013): 52. http://dx.doi.org/10.1186/scrt202.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Underwood, Clayton J., Lowell T. Edgar, James B. Hoying, and Jeffrey A. Weiss. "Cell-generated traction forces and the resulting matrix deformation modulate microvascular alignment and growth during angiogenesis." American Journal of Physiology-Heart and Circulatory Physiology 307, no. 2 (2014): H152—H164. http://dx.doi.org/10.1152/ajpheart.00995.2013.

Full text
Abstract:
The details of the mechanical factors that modulate angiogenesis remain poorly understood. Previous in vitro studies of angiogenesis using microvessel fragments cultured within collagen constructs demonstrated that neovessel alignment can be induced via mechanical constraint of the boundaries (i.e., boundary conditions). The objective of this study was to investigate the role of mechanical boundary conditions in the regulation of angiogenic alignment and growth in an in vitro model of angiogenesis. Angiogenic microvessels within three-dimensional constructs were subjected to different boundary conditions, thus producing different stress and strain fields during growth. Neovessel outgrowth and orientation were quantified from confocal image data after 6 days. Vascularity and branching decreased as the amount of constraint imposed on the culture increased. In long-axis constrained hexahedral constructs, microvessels aligned parallel to the constrained axis. In contrast, constructs that were constrained along the short axis had random microvessel orientation. Finite element models were used to simulate the contraction of gels under the various boundary conditions and to predict the local strain field experienced by microvessels. Results from the experiments and simulations demonstrated that microvessels aligned perpendicular to directions of compressive strain. Alignment was due to anisotropic deformation of the matrix from cell-generated traction forces interacting with the mechanical boundary conditions. These findings demonstrate that boundary conditions and thus the effective stiffness of the matrix regulate angiogenesis. This study offers a potential explanation for the oriented vascular beds that occur in native tissues and provides the basis for improved control of tissue vascularization in both native tissues and tissue-engineered constructs.
APA, Harvard, Vancouver, ISO, and other styles
7

Muscari, Claudio, Emanuele Giordano, Francesca Bonafè, Marco Govoni, and Carlo Guarnieri. "Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering." Stem Cells International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/434169.

Full text
Abstract:
The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. In addition to these basic approaches, more sophisticated strategies have been followed employing mixed-cell sheets, microvascular modules, and inosculation from vascular explants. Technologies exerting spatial control of vascular cells, such as topographical surface roughening and ordered patterning, represent other ways to drive scaffold vascularization. Finally, microfluidic devices and bioreactors exerting mechanical stress have also been employed for high-throughput scaling-up production in order to accelerate muscle differentiation and speeding the endothelialization process. Future research should address issues such as how to optimize cells, biomaterials, and biochemical components to improve the vascular integration of the construct within the cardiac wall, satisfying the metabolic and functional needs of the myocardial tissue.
APA, Harvard, Vancouver, ISO, and other styles
8

Atlas, Yoann, Caroline Gorin, Anita Novais, et al. "Microvascular maturation by mesenchymal stem cells in vitro improves blood perfusion in implanted tissue constructs." Biomaterials 268 (January 2021): 120594. http://dx.doi.org/10.1016/j.biomaterials.2020.120594.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Medhora, Meetha, John Daniels, Kavita Mundey, et al. "Epoxygenase-driven angiogenesis in human lung microvascular endothelial cells." American Journal of Physiology-Heart and Circulatory Physiology 284, no. 1 (2003): H215—H224. http://dx.doi.org/10.1152/ajpheart.01118.2001.

Full text
Abstract:
Angiogenesis is one of the most recent physiological functions attributed to products of cytochrome P-450 (CYP450) enymes. To test this at a molecular level in human cells, we used a cloned cDNA for the human endothelial enzyme CYP450 2C9 (CYP2C9) to study growth as well as differentiation of human microvascular endothelial cells from the lung (HMVEC-L). Using adenoviral vectors overexpressing mRNA for CYP2C9, we show that the presence of CYP2C9 doubles thymidine incorporation and stimulates proliferation of primary cultures of endothelial cells compared with Ad5-GFP (control) in 24 h. In addition, there is a significant increase of tube formation in Matrigel after infection of HMVEC-L with Ad5-2C9 than with Ad5-GFP. More interestingly, Ad5-2C9 expressing the antisense product of CYP2C9 (2C9AS) inhibited tube formation compared with both Ad5-GFP as well as the Ad5-2C9 constructs. Finally, we tested the most abundant arachidonic acid metabolite of CYP2C9, 14,15-epoxyeicosatrienoic acid, which induced angiogenesis in vivo when embedded in Matrigel plugs and implanted in adult rats. These data support an important role for CYP2C9 in promoting angiogenesis.
APA, Harvard, Vancouver, ISO, and other styles
10

Muehlich, Susanne, Iwona Cicha, Christoph D. Garlichs, Bettina Krueger, Guido Posern, and Margarete Goppelt-Struebe. "Actin-dependent regulation of connective tissue growth factor." American Journal of Physiology-Cell Physiology 292, no. 5 (2007): C1732—C1738. http://dx.doi.org/10.1152/ajpcell.00552.2006.

Full text
Abstract:
Expression of connective tissue growth factor (CTGF) in endothelial cells is modulated by shear stress affecting the organization of the cytoskeleton. The molecular connection between alterations of actin and CTGF expression was investigated in human umbilical vein endothelial cells (HUVEC) and a microvascular endothelial cell line. Overexpression of nonpolymerizable monomeric actin R62D interfered with stress fiber formation in HUVEC and concomitantly reduced immunoreactive CTGF. In microvascular endothelial cells, flow-dependent upregulation of CTGF was prevented by this actin mutant. In contrast, overexpression of actin S14C strengthened filamentous actin and increased CTGF expression. These data indicated an inverse relationship between CTGF expression and monomeric actin. Coexpression of the mutant actins and different CTGF promoter constructs revealed an actin-sensitive site between 3 and 4.5 kb of the CTGF promoter. A CArG-like box at −3791 bp was responsible for actin-dependent CTGF induction as shown by mutagenesis. Overexpression of actin S14C activated the nonmutated promoter significantly more strongly than the mutated promoter. Actin polymerization is regulated by the small GTPase RhoA and activation of serum response factor (SRF). Overexpression of constitutively active RhoA or SRF significantly increased CTGF protein synthesis. The 4.5-kb promoter construct, but not the construct with a mutation in the CArG box, was activated by SRF or RhoA, providing evidence for a functional role of this site in CTGF induction. These findings provide novel evidence that monomeric actin is the connecting link between alterations in the cytoskeleton and CTGF gene expression and demonstrate the importance of SRF in regulating CTGF transcription.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Microvascular constructs"

1

Naik, Nisarga. "MEMS-based nozzles and templates for the fabrication of engineered tissue constructs." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/42853.

Full text
Abstract:
This dissertation presents the application of MEMS-based approaches for the construction of engineered tissue substitutes. MEMS technology can offer the physical scale, resolution, and organization necessary for mimicking native tissue architecture. Micromachined nozzles and templates were explored for the fabrication of acellular, biomimetic collagenous fibrous scaffolds, microvascular tissue structures, and the combination of these structures with cell-based therapeutics. The influence of the microstructure of the tissue constructs on their macro-scale characteristics was investigated.
APA, Harvard, Vancouver, ISO, and other styles
2

Li, Mon Tzu. "Treatment strategy for composite tissue limb trauma." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54837.

Full text
Abstract:
A majority of all fractures in current US armed conflicts are open fractures, in which a soft tissue injury is sustained along with the bone fracture. Even with gold standard treatment, in which muscle flaps are used to cover bony defects, patients often do not regain normal function of their extremity, highlighting the necessity for tissue engineering strategies for this complex clinical problem. Due to a substantial amount of tissue damage and debridement treatment in composite injuries, a large volume of cells and extracellular matrix (ECM) proteins that are necessary for tissue healing are removed from the body. In the replacement of large volumes of tissue, nutrient transfer necessitates a vascular supply to maintain the viability of delivered cells. The objective of this project was to examine the regenerative potential of engineered matrix constructs and stem cells on composite bone & muscle defects. We hypothesized that stem cells delivered on engineered matrix constructs into the muscle defect will aid in muscle regeneration and promote bone healing, ultimately resulting in superior functional limb recovery. These studies established multiple preclinical platforms for testing tissue engineering strategies as well as models that can be used to gain insights on the healing of VML and composite VML/bone defects. From some of the insights gained on the vascularization of the defect sites, a vascular treatment strategy was tested within these platforms and shown to have varying results in the treatment of complex multi-tissue injuries.
APA, Harvard, Vancouver, ISO, and other styles
3

Huang, Jen-Huang. "Novel Methods to Construct Microchannel Networks with Complex Topologies." Thesis, 2012. http://hdl.handle.net/1969.1/148269.

Full text
Abstract:
Microfluidic technology is a useful tool to help answer unsolved problems in multidisciplinary fields, including molecular biology, clinical pathology and the pharmaceutical industry.Current microfluidic based devices with diverse structures have been constructed via extensively used soft lithography orphotolithography fabrication methods. A layer-by-layer stacking of 2D planar microchannel arrays can achieve limited degrees of three dimensionality. However, assembly of large-scale multi-tiered structures is tedious, and the inherently planar nature of the individual layers restricts the network’s topological complexity. In order to overcome the limitations of existing microfabrication methodswe demonstrate several novel methods that enable microvasculature networks: electrostatic discharge,global channel deformation and enzymatic sculpting to fabricate complex surface topologies. These methods enable construction of networks of branched microchannels arranged in a tree-like architecture with diameters ranging from approximately 10 μm to 1 mm. Interconnected networks with multiple fluidic access points can be straightforwardly constructed, and quantification of their branching characteristics reveals remarkable similarity to naturally occurring vasculature. In addition, by harnessing enzymatic micromachining we are able to construct nanochannels, microchannels containing embedded features templated by the substrate’s crystalline morphology, and an irregular cross section of microchannel capable of performing isolation and enrichment of cells from whole blood with throughput 1 – 2 orders of magnitude faster than currently possible. These techniques can play a key role in developing an organ-sized engineered tissue scaffolds and high-throughput continuous flow separations.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Microvascular constructs"

1

T., Mani, Stefanie V., and John W. "3-D Microvascular Tissue Constructs for Exploring Concurrent Temporal and Spatial Regulation of Postnatal Neovasculogenesis." In Research Directions in Tumor Angiogenesis. InTech, 2013. http://dx.doi.org/10.5772/53118.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Microvascular constructs"

1

Krishnan, Laxminarayanan, Carlos C. Chang, Shawn Reese, Stuart K. Williams, Jeffrey A. Weiss, and James B. Hoying. "Anchorage: Dependent Persistent Alignment of Perfused Microvasculature in Implanted Tissue Constructs." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53630.

Full text
Abstract:
One of the challenges in engineering complex artificial tissue constructs, with defined matrix and cellular architecture, is the formation of a viable microcirculation within them, that inosculates with the host vasculature and matures into a functional microvascular bed. Current methods based on complex cell patterning in 2-D or 3-D matrix systems rely on ‘printing’ cells or patterns of cells on/ in a substrate, direct culture on patterned substrates, or endothelialization of decellularized vessels. We are now beginning to understand the effects of the microenvironment on microvascular constructs. Flow induced remodeling and maturation of angiogenic microvasculature and changes in functional characteristics when co-implanted with astrocyte precursors strongly suggests a role for the local environment in determining characteristics of the microvascular bed [1, 2]. Chang et al. have shown that neovascular networks from microvessels pre-aligned by direct-bioprinting in a collagen matrix, retain alignment when cultured in vitro, but lose alignment on implantation in vivo [3]. Though unloading of mechanically loaded tissue influences cell behavior [4], it does not explain this loss of orientation after implantation, of previously unloaded constructs. Implanted constructs have an additional level of complexity in the form of network revision and maturation with blood flow. We hypothesize that the local mechanical microenvironment, in addition to flow, dictates network morphology in vivo. This study compares the changes in pre-aligned microvascular networks implanted with and without anchorage.
APA, Harvard, Vancouver, ISO, and other styles
2

Emerson, David R., and Robert W. Barber. "Designing Efficient Microvascular Networks Using Conventional Microfabrication Techniques." In ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer. ASMEDC, 2009. http://dx.doi.org/10.1115/mnhmt2009-18312.

Full text
Abstract:
The ability to fabricate networks of micro-channels that obey the biological properties of bifurcating structures found in nature suggests that it is possible to construct artificial vasculatures or bronchial structures. These devices could aid in the desirable objective of eliminating many forms of animal testing. In addition, the ability to precisely control hydraulic conductance could allow designers to create specific concentration gradients that would allow biologists to correlate the behavior of cells. In 1926, Murray found that there was an optimum branching ratio between the diameters of the parent and daughter vessels at a bifurcation. For biological vascular systems, this is referred to as Murray’s law and its basic principle has been found to be valid in many plant and mammalian organisms. An important consequence arises from this law: when the successive generations consist of regular dichotomies, the tangential shear stress at the wall remains constant throughout the network. This simple concept forms an elegant biomimetic design rule that will allow designers to create complex sections with the desired hydraulic conductance or resistance. The paper presents a theoretical analysis of how biomimetic networks of constant-depth rectangular channels can be fabricated using standard photolithographic techniques. In addition, the design rule developed from Murray’s law is extended to a simple power-law fluid to investigate whether it is feasible to design biomimetic networks for non-Newtonian liquids. Remarkably, Murray’s law is obeyed for power-law fluids in cylindrical pipes. Although highly promising, the extension of the analysis to rectangular or trapezoidal channels requires much further work. Moreover, it is unclear at this stage whether Murray’s law holds for other non-Newtonian models.
APA, Harvard, Vancouver, ISO, and other styles
3

Zhou, Qinlian, Jian Gao, Wei Huang, and R. T. Yen. "Vascular Impedance Analysis in Human Pulmonary Circulation." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-33525.

Full text
Abstract:
Vascular impedance in human pulmonary circulation is analyzed by the fluid dynamic approach. A model representing the entire system of pulmonary circulation is constructed based on experimentally measured morphometric and elasticity data of the vessels. The pulmonary arteries and veins are considered as elastic tubes. Their impedance follows Womersley’s theory and electric analogue. The “sheet-flow” theory is employed to describe the flow in capillaries and thus a microvascular impedance matrix is derived. The input impedance at the main pulmonary artery is calculated under both zone 3 and zone 2 conditions. The results are compared with available experimental data in the literature.
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!