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Journal articles on the topic 'Mineralized differentiation'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Adekeye, J. I. D., and A. D. Adedoyin. "ECONOMIC POTENTIALS OF THE PEGMATITES OF ERUKU AREA, SOUTHWESTERN NIGERIA." Continental J. Earth Sciences 2 (November 7, 2007): 1–6. https://doi.org/10.5281/zenodo.824279.

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The close proximity of the pegmatites of Eruku area to the strongly mineralized pegmatites of Egbe area and lack of published work on those from the former formed the basis of this research. Sub-parallel, steeply-dipping, lenticular/podlike pegmatite bodies occur in the Eruku area, southwestern Nigeria. They are emplaced within the late Proterozoic to early Paleozoic crystalline basement complex rocks. Some of these pegmatites are mineralized while others are barren. The barren pegmatites are sources of industrial minerals like feldspar and quartz. The mineralized pegmatites are also sources o

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Liu, Wei, Xiaowei Jing, Zhiwen Xu, and Chong Teng. "PEGDA/HA mineralized hydrogel loaded with Exendin4 promotes bone regeneration in rat models with bone defects by inducing osteogenesis." Journal of Biomaterials Applications 35, no. 10 (2021): 1337–46. http://dx.doi.org/10.1177/0885328220987046.

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Scaffolds with osteogenic differentiation function play an important role in the healing process of bone defects. Here, we designed a high strength Poly(ethyleneglycol) diacrylate/Hydroxyapatite (PEGDA/HA) mineralized hydrogel loaded with Exendin4 for inducing osteogenic differentiation. In this study, PEGDA hydrogel was prepared by photo initiating method. PEGDA/HA mineralized hydrogel was prepared by in-situ precipitation method, and Exendin4 was loaded by gel adsorption. The effects of different calcium and phosphorus concentrations on the strength and Exendin4 release of PEGDA/HA hydrogels

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Dewey, Marley J., Eileen M. Johnson, Simona T. Slater, Derek J. Milner, Matthew B. Wheeler, and Brendan A. C. Harley. "Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions." Regenerative Biomaterials 7, no. 3 (2020): 247–58. http://dx.doi.org/10.1093/rb/rbaa005.

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Abstract Defects in craniofacial bones occur congenitally, after high-energy impacts, and during the course of treatment for stroke and cancer. These injuries are difficult to heal due to the overwhelming size of the injury area and the inflammatory environment surrounding the injury. Significant inflammatory response after injury may greatly inhibit regenerative healing. We have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental proteins. The amniotic membrane is derived from placentas

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Eren, E. Deniz, Gulistan Tansik, Ayse B. Tekinay, and Mustafa O. Guler. "Mineralized Peptide Nanofiber Gels for Enhanced Osteogenic Differentiation." ChemNanoMat 4, no. 8 (2018): 837–45. http://dx.doi.org/10.1002/cnma.201700354.

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5

Liu, Yangxi, Jue Hu, and Hongli Sun. "Mineralized nanofibrous scaffold promotes phenamil‐induced osteoblastic differentiation while mitigating adipogenic differentiation." Journal of Tissue Engineering and Regenerative Medicine 14, no. 3 (2020): 464–74. http://dx.doi.org/10.1002/term.3007.

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6

Bjørge, Isabel M., Insung S. Choi, Clara R. Correia, and João F. Mano. "Nanogrooved microdiscs for bottom-up modulation of osteogenic differentiation." Nanoscale 11, no. 35 (2019): 16214–21. http://dx.doi.org/10.1039/c9nr06267j.

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Novel disc-like microparticles, herein termed as “topodiscs”, with grooved surface nanotopography effectively enhanced cell culture and allowed for a bottom-up engineering of 3D mineralized microtissues.

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Ren, Xiaoyan, Qi Zhou, David Foulad, et al. "Osteoprotegerin reduces osteoclast resorption activity without affecting osteogenesis on nanoparticulate mineralized collagen scaffolds." Science Advances 5, no. 6 (2019): eaaw4991. http://dx.doi.org/10.1126/sciadv.aaw4991.

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The instructive capabilities of extracellular matrix–inspired materials for osteoprogenitor differentiation have sparked interest in understanding modulation of other cell types within the bone regenerative microenvironment. We previously demonstrated that nanoparticulate mineralized collagen glycosaminoglycan (MC-GAG) scaffolds efficiently induced osteoprogenitor differentiation and bone healing. In this work, we combined adenovirus-mediated delivery of osteoprotegerin (AdOPG), an endogenous anti-osteoclastogenic decoy receptor, in primary human mesenchymal stem cells (hMSCs) with MC-GAG to u

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Park, Kyung-Ran, Joon Yeop Lee, Myounglae Cho, Jin Tae Hong, and Hyung-Mun Yun. "Biological Mechanisms of Paeonoside in the Differentiation of Pre-Osteoblasts and the Formation of Mineralized Nodules." International Journal of Molecular Sciences 22, no. 13 (2021): 6899. http://dx.doi.org/10.3390/ijms22136899.

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Paeonia suffruticosa is a magnificent and long-lived woody plant that has traditionally been used to treat various diseases including inflammatory, neurological, cancer, and cardiovascular diseases. In the present study, we demonstrated the biological mechanisms of paeonoside (PASI) isolated from the dried roots of P. suffruticosa in pre-osteoblasts. Herein, we found that PASI has no cytotoxic effects on pre-osteoblasts. Migration assay showed that PASI promoted wound healing and transmigration in osteoblast differentiation. PASI increased early osteoblast differentiation and mineralized nodul

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9

Weisgerber, Daniel W., Steven R. Caliari, and Brendan A. C. Harley. "Mineralized collagen scaffolds induce hMSC osteogenesis and matrix remodeling." Biomaterials Science 3, no. 3 (2015): 533–42. http://dx.doi.org/10.1039/c4bm00397g.

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Incorporating calcium phosphate nanocrystallites into a collagen biomaterial promotes mesenchymal stem cell (MSC) osteogenic differentiation in the absence of conventional growth factor supplements. Mineralized collagen scaffolds also support MSC proliferation and new matrix biosynthesis.

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10

Apel, Christian, Patricia Buttler, Jochen Salber, Anandhan Dhanasingh, and Sabine Neuss. "Differential mineralization of human dental pulp stem cells on diverse polymers." Biomedical Engineering / Biomedizinische Technik 63, no. 3 (2018): 261–69. http://dx.doi.org/10.1515/bmt-2016-0141.

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Abstract In tissue engineering, biomaterials are used as scaffolds for spatial distribution of specific cell types. Biomaterials can potentially influence cell proliferation and extracellular matrix formation, both in positive and negative ways. The aim of the present study was to investigate and compare mineralized matrix production of human dental pulp stem cells (DPSC), cultured on 17 different well-characterized polymers. Osteogenic differentiation of DPSC was induced for 21 days on biomaterials using dexamethasone, L-ascorbic-acid-2-phosphate, and sodium β-glycerophosphate. Success of dif

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11

Van Vlasselaer, P., B. Borremans, R. Van Den Heuvel, U. Van Gorp, and R. de Waal Malefyt. "Interleukin-10 inhibits the osteogenic activity of mouse bone marrow." Blood 82, no. 8 (1993): 2361–70. http://dx.doi.org/10.1182/blood.v82.8.2361.2361.

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Abstract Murine bone marrow cells synthesize bone proteins, including alkaline phosphatase (ALP), collagen type I, and osteocalcin, and form a mineralized extracellular matrix when cultured in the presence of beta- glycerophosphate and vitamin C. Interleukin-10 (IL-10) suppressed the synthesis of these bone proteins and mineralization without affecting cell proliferation. In addition, mRNA levels for the latter proteins were reduced in IL-10-treated cultures. This inhibitory effect was most outspoken when IL-10 was added before ALP activity peaked, eg, day 15 of culture. No significant effect

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Van Vlasselaer, P., B. Borremans, R. Van Den Heuvel, U. Van Gorp, and R. de Waal Malefyt. "Interleukin-10 inhibits the osteogenic activity of mouse bone marrow." Blood 82, no. 8 (1993): 2361–70. http://dx.doi.org/10.1182/blood.v82.8.2361.bloodjournal8282361.

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Murine bone marrow cells synthesize bone proteins, including alkaline phosphatase (ALP), collagen type I, and osteocalcin, and form a mineralized extracellular matrix when cultured in the presence of beta- glycerophosphate and vitamin C. Interleukin-10 (IL-10) suppressed the synthesis of these bone proteins and mineralization without affecting cell proliferation. In addition, mRNA levels for the latter proteins were reduced in IL-10-treated cultures. This inhibitory effect was most outspoken when IL-10 was added before ALP activity peaked, eg, day 15 of culture. No significant effect was obser

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13

zur Nieden, Nicole I., Grazyna Kempka, and Hans J. Ahr. "In vitro differentiation of embryonic stem cells into mineralized osteoblasts." Differentiation 71, no. 1 (2003): 18–27. http://dx.doi.org/10.1046/j.1432-0436.2003.700602.x.

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14

Luo, L., H. Uludag, E. Sone, and S. Viswanathan. "Biomimetic mineralized collagen scaffolds and their effect on osteogenic differentiation." Cytotherapy 22, no. 5 (2020): S183. http://dx.doi.org/10.1016/j.jcyt.2020.03.384.

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15

Ghoul-Mazgar, Sonia, Dominique Hotton, Frédéric Lézot, et al. "Expression pattern of Dlx3 during cell differentiation in mineralized tissues." Bone 37, no. 6 (2005): 799–809. http://dx.doi.org/10.1016/j.bone.2005.03.020.

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16

Zhu, H. B., D. Z. Guo, S. J. Yang, et al. "Osteogenic actions of the osteogenic growth peptide on bovine marrow mesenchymal stromal cells in culture." Veterinární Medicína 53, No. 9 (2008): 501–9. http://dx.doi.org/10.17221/1981-vetmed.

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The osteogenic growth peptide (OGP) regulates the differentiation of marrow mesenchymal stem cells derived from human and rodent cell lines into osteoblasts. Whether OGP directly regulates the bovine marrow mesenchymal stem cells differentiating into osteoblasts remains unknown. In this study, we evaluated the effects of OGP on the growth and differentiation of bovine marrow mesenchymal stem cells in culture. Our results showed that OGP promoted osteogenic differentiation of the bovine stem cells. OGP increased alkaline phosphatase (ALP) activity and mineralized nodule formation, and stimulate

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17

Yamamoto, Maiko, Hidemi Nakata, Jia Hao, Joshua Chou, Shohei Kasugai, and Shinji Kuroda. "Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro." Stem Cells International 2014 (2014): 1–17. http://dx.doi.org/10.1155/2014/576358.

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Adipose tissue-derived stromal cells, termed ASCs, play an important role in regenerative applications. They resemble mesenchymal stem cells owing to their inexhaustibility, general differentiation potential, and plasticity and display a series of cell-specific and cluster-of-differentiation (CD) marker profiles similar to those of other somatic stem cells. Variations in phenotypes or differentiation are intimately associated with CD markers. The purpose of our study was to exhibit distinct populations of ASCs with differing characteristics for osteogenic differentiation. The primary cell batc

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18

Liu, Guolin, Guoquan Xu, Zhenhua Gao, et al. "Demineralized Dentin Matrix Induces Odontoblastic Differentiation of Dental Pulp Stem Cells." Cells Tissues Organs 201, no. 1 (2015): 65–76. http://dx.doi.org/10.1159/000440952.

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The aim of this study was to investigate the effect of demineralized dentin matrix (DDM) on dental pulp stem cells (DPSCs) and the potential of complexes with DPSCs and DDM for mineralized tissue formation. Stem cells derived from the dental pulp of healthy pigs aged 18 months were isolated and cultured. DPSCs were incubated with alpha-minimum essential medium treated with DDM extract at 1 mg/ml (DDM1) or 10 mg/ml (DDM10). The concentrations of 3 growth factors in DDM extract was measured by enzyme-linked immunosorbent assay. Adhesion of DPSCs on DDM and hydroxyapatite-tricalcium phosphate (HA

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19

Ramírez-Rodríguez, Gloria Belén, Ana Rita Pereira, Marietta Herrmann, et al. "Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor." International Journal of Molecular Sciences 22, no. 3 (2021): 1447. http://dx.doi.org/10.3390/ijms22031447.

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In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The s

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20

Jenkins, Tiffany L., Dalen Agnew, and Daniel R. Rissi. "Fibroblastic osteosarcoma with epithelioid and squamous differentiation in a dog." Journal of Veterinary Diagnostic Investigation 30, no. 4 (2018): 593–97. http://dx.doi.org/10.1177/1040638718768373.

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A fibroblastic osteosarcoma with epithelioid and squamous differentiation in the distal femur of a 9-y-old spayed female Greyhound dog is described. Grossly, the tumor consisted of a pale-white, firm-to-hard mass that replaced the medullary and cortical areas of the distal end of the right femur. Histologically, the mass was composed predominantly of spindle cells admixed with areas of mineralized and non-mineralized osteoid matrix that were surrounded by stellate osteoblasts and scattered multinucleate giant cells, consistent with the diagnosis of a fibroblastic osteosarcoma. In addition, wel

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21

Roza Llera, Ana, Mercedes Fuertes-Fuente, Antonia Cepedal, and Agustín Martin-Izard. "Barren and Li–Sn–Ta Mineralized Pegmatites from NW Spain (Central Galicia): A Comparative Study of Their Mineralogy, Geochemistry, and Wallrock Metasomatism." Minerals 9, no. 12 (2019): 739. http://dx.doi.org/10.3390/min9120739.

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In Central Galicia, there are occurrences of barren and Li–Sn–Ta-bearing pegmatites hosted by metasedimentary rocks. A number of common and contrasting features between Panceiros pegmatites (barren) and Li–Sn–Ta mineralized Presqueira pegmatite are established in this study. K-feldspar and muscovite have the same trace elements (Rb, Cs, P, Zn, and Ba), but the mineralized one has the highest Rb and Cs and the lowest P contents. The barren bodies show fluorapatite and eosphorite–childrenite replacing early silicates. The mineralized body has primary phosphates (fluorapatite and montebrasite), a

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22

Park, Eunkuk, Jeonghyun Kim, Mun-Chang Kim, et al. "Anti-Osteoporotic Effects of Kukoamine B Isolated from Lycii Radicis Cortex Extract on Osteoblast and Osteoclast Cells and Ovariectomized Osteoporosis Model Mice." International Journal of Molecular Sciences 20, no. 11 (2019): 2784. http://dx.doi.org/10.3390/ijms20112784.

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Osteoporosis is an abnormal bone remodeling condition characterized by decreased bone density, which leads to high risks of fracture. Previous study has demonstrated that Lycii Radicis Cortex (LRC) extract inhibits bone loss in ovariectomized (OVX) mice by enhancing osteoblast differentiation. A bioactive compound, kukoamine B (KB), was identified from fractionation of an LRC extract as a candidate component responsible for an anti-osteoporotic effect. This study investigated the anti-osteoporotic effects of KB using in vitro and in vivo osteoporosis models. KB treatment significantly increase

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Westhrin, Marita, Minli Xie, Magnus Ø. Olderøy, Pawel Sikorski, Berit L. Strand, and Therese Standal. "Osteogenic Differentiation of Human Mesenchymal Stem Cells in Mineralized Alginate Matrices." PLOS ONE 10, no. 3 (2015): e0120374. http://dx.doi.org/10.1371/journal.pone.0120374.

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24

Sasaki, Takahisa. "Differentiation and functions of osteoclasts and odontoclasts in mineralized tissue resorption." Microscopy Research and Technique 61, no. 6 (2003): 483–95. http://dx.doi.org/10.1002/jemt.10370.

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25

Song, Ke Dong, Xiang Qin Li, Shi Xiao Li, et al. "Osteogenic Differentiation of ADSCs in Novel Microbeads in Dynamic Environment." Advanced Materials Research 997 (August 2014): 233–38. http://dx.doi.org/10.4028/www.scientific.net/amr.997.233.

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Feasibility of constructing bone microbeads by culturing encapsulated ADSCs in calcium alginate/bone powder (CABP) microbeads in spinner flask was explored. Experimental group was osteogenic differentiation of encapsulated ADSCs in CABP microbeads in spinner flask, control group I and II were osteogenic differentiation of ADSCs encapsulated in CABP microbeads and in calcium alginate microbeads in T-flasks, respectively. Growth status of encapsulated ADSCs in beads was observed by Calcein-AM/PI Staining. After 14 days of induction, ALP staining was used to qualitatively detect ALP in culture me

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Domingos Neto, Benedito, Victória Maria Hebling, Gabriela Gomes Cardoso Gastaldi, Heloisa Sobreiro Selistre de Araújo, Eliane Trovatti, and Mônica Rosas da Costa Iemma. "Induction of mineralized matrix production by recombinant human BMP-2 Immobilized in TEMPO-Oxidized Cellulose Hydrogel: a novel target for tissue repair." International Journal of Advances in Medical Biotechnology - IJAMB 6, no. 1 (2024): 34–42. http://dx.doi.org/10.52466/ijamb.v6i1.124.

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Bone morphogenetic proteins (BMPs) are potent promoters of osteogenesis, especially BMP-2, which has been highlighted for acting as a growth and differentiation factor that promotes new bone formation. There are several biomaterials that can be used to release bioactive substances, such as natural polymers. Cellulose has stood out for the possibility of its chemical modification using the reagent 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) to obtain a cellulose derivative (TEMPO oxidized cellulose nanofibers - ToCNF), which is shown to be a promising material for biological application. The o

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Takeuchi, Kyoko, Masahiro Abe, Asuka Oda, et al. "SB431542, a TGF-Beta Receptor Kinase Inhibitor, Restores Bone Formation Which Ameliorates Myeloma-Induced Microenvironment." Blood 108, no. 11 (2006): 3479. http://dx.doi.org/10.1182/blood.v108.11.3479.3479.

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Abstract Multiple myeloma (MM) cells stimulate bone resorption and concomitantly suppress bone formation, leading to devastating bone destruction. TGF-beta, a potent inhibitor for terminal osteoblasts (OB) maturation and mineralization, is abundantly produced and released from bone tissues through enhanced bone resorption, and activated by osteoclasts (OC)-derived acids and MMPs in MM bone lesions. In the present study we investigated the impact of TGF-beta inhibition on induction of bone formation in MM as well as the effects of matured OB on MM growth. TGF-beta completely suppressed BMP-2-in

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Chen, D., X. Ji, M. A. Harris, et al. "Differential Roles for Bone Morphogenetic Protein (BMP) Receptor Type IB and IA in Differentiation and Specification of Mesenchymal Precursor Cells to Osteoblast and Adipocyte Lineages." Journal of Cell Biology 142, no. 1 (1998): 295–305. http://dx.doi.org/10.1083/jcb.142.1.295.

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Cumulative evidence indicates that osteoblasts and adipocytes share a common mesenchymal precursor and that bone morphogenetic proteins (BMPs) can induce both osteoblast and adipocyte differentiation of this precursor. In the present study, we investigated the roles of BMP receptors in differentiation along these separate lineages using a well-characterized clonal cell line, 2T3, derived from the mouse calvariae. BMP-2 induced 2T3 cells to differentiate into mature osteoblasts or adipocytes depending upon culture conditions. To test the specific roles of the type IA and IB BMP receptor compone

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Sancilio, Silvia, Eleonora Marsich, Helmut Schweikl, Amelia Cataldi, and Marialucia Gallorini. "Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth." Nanomaterials 9, no. 12 (2019): 1656. http://dx.doi.org/10.3390/nano9121656.

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Composites and porous scaffolds produced with biodegradable natural polymers are very promising constructs which show high biocompatibility and suitable mechanical properties, with the possibility to be functionalized with growth factors involved in bone formation. For this purpose, alginate/hydroxyapatite (Alg/HAp) composite scaffolds using a novel production design were successfully developed and tested for their biocompatibility and osteoconductive properties in vitro. Redox homeostasis is crucial for dental pulp stem cell (DPSC) differentiation and mineralized matrix deposition, and interl

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Gao, Bingjun, Yarong Wu, Lijian Zhou, and Xin Chen. "MicroRNA-595 promotes osteogenic differentiation of bone marrow mesenchymal stem cells by targeting HMGA2." Tropical Journal of Pharmaceutical Research 21, no. 3 (2022): 457–63. http://dx.doi.org/10.4314/tjpr.v21i3.1.

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Purpose: To investigate the effect of miR-595 on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).Methods: Human BMSCs were osteogenically differentiated, and protein expression of alkaline phosphatase (ALP), osteocalcin (OCN), and Runt-related transcription factor 2 (RUNX2) were evaluated by western blot. Expression of miR-595 was measured by quantitative reverse transcription (qRT-PCR). The effect of miR-595 on viability of BMSCs was determined by MTT assay. Osteogenic differentiation of BMSCs was assessed by ALP and Alizarin red S (ARS) staining. The target gene of m

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Nefussi, Jean R., Gabriel Brami, Dominique Modrowski, Martine Obcuf, and Nadine Forest. "Sequential Expression of Bone Matrix Proteins During Rat Calvaria Osteoblast Differentiation and Bone Nodule Formation In Vitro." Journal of Histochemistry & Cytochemistry 45, no. 4 (1997): 493–503. http://dx.doi.org/10.1177/002215549704500402.

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We investigated the expression of osteocalcin (OC), bone sialoprotein (BSP), osteonectin (ON), and alkaline phosphatase (ALP) during cell differentiation and bone nodule formation by fetal rat calvaria cells, using immunofluorescent and immunogold techniques at light and electron microscopic levels. Six hours after plating all proteins were expressed in calvaria cells. However, expression was not detected during the proliferation phase after plating. Cell morphological modifications were observed in osteoblastic cells expressing ALP, OC, and BSP, but not ON. During the matrix formation phase,

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Seiffert, D. "Detection of vitronectin in mineralized bone matrix." Journal of Histochemistry & Cytochemistry 44, no. 3 (1996): 275–80. http://dx.doi.org/10.1177/44.3.8648088.

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Adhesive glycoproteins in the bone matrix are of critical importance for cell anchorage, proliferation, migration, differentiation, and regulation of bone metabolism. The localization of the adhesive glycoprotein vitronectin (Vn) in murine bone tissue was evaluated by immunohistochemical staining. Vitronectin was present throughout the mineralized bone matrix of cancellous and cortical bone, whereas cartilage was devoid of Vn staining. To exclude the possibility that the positive Vn staining resulted from plasma Vn in blood vessels within the bone sections, adjacent tissue sections were staine

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Xu, Su-Ju, Zhi-Ye Qiu, Jing-Jing Wu, et al. "Osteogenic Differentiation Gene Expression Profiling of hMSCs on Hydroxyapatite and Mineralized Collagen." Tissue Engineering Part A 22, no. 1-2 (2016): 170–81. http://dx.doi.org/10.1089/ten.tea.2015.0237.

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Li, Xiaofeng, Peng Liu, Wenzhong Liu, et al. "Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation." Nature Genetics 37, no. 9 (2005): 945–52. http://dx.doi.org/10.1038/ng1614.

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Ambre, Avinash H., Dinesh R. Katti, and Kalpana S. Katti. "Nanoclays mediate stem cell differentiation and mineralized ECM formation on biopolymer scaffolds." Journal of Biomedical Materials Research Part A 101A, no. 9 (2013): 2644–60. http://dx.doi.org/10.1002/jbm.a.34561.

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36

Choi, J. Y., A. J. van Wijnen, F. Aslam, et al. "Developmental association of the beta-galactoside-binding protein galectin-1 with the nuclear matrix of rat calvarial osteoblasts." Journal of Cell Science 111, no. 20 (1998): 3035–43. http://dx.doi.org/10.1242/jcs.111.20.3035.

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The protein composition of the nuclear matrix changes significantly as the osteoblast matures from a proliferating pre-osteoblast to an osteocyte embedded in a mineralized matrix. These matrix protein are the result of developmental stage-specific gene expression during osteoblast differentiation. To isolate nuclear matrix proteins unique to the bone phenotype we analyzed nuclear matrix preparations from cultures of rat calvarial osteoblasts by high resolution two-dimensional gel electrophoresis at two different stages: proliferation (day 3) and differentiation (day 18, mineralized). We charac

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Choi, Je-Yong, André J. van Wijnen, Fauzia Aslam та ін. "Developmental association of the β-galactoside-binding protein galectin-1 with the nuclear matrix of rat calvarial osteoblasts". Journal of Cell Science 111, № 20 (1998): 3035–43. http://dx.doi.org/10.1242/jcs.20.111.3035.

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ABSTRACT The protein composition of the nuclear matrix changes significantly as the osteoblast matures from a proliferating pre-osteoblast to an osteocyte embedded in a mineralized matrix. These matrix protein are the result of developmental stage-specific gene expression during osteoblast differentiation. To isolate nuclear matrix proteins unique to the bone phenotype we analyzed nuclear matrix preparations from cultures of rat calvarial osteoblasts by high resolution two-dimensional gel electrophoresis at two different stages: proliferation (day 3) and differentiation (day 18, mineralized).

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Moon, Jung-Sun, Seong-Duk Kim, Hyun-Mi Ko, Young-Jun Kim, Sun-Hun Kim, and Min-Seok Kim. "Twist1 Suppresses Cementoblast Differentiation." Dentistry Journal 6, no. 4 (2018): 57. http://dx.doi.org/10.3390/dj6040057.

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The transcription factor Twist1 is known to be closely associated with the formation of bone by mesenchymal stem cells and osteoblasts; however, the role of Twist1 in cementogenesis has not yet been determined. This study was undertaken to elucidate the roles of Twist1 in cementoblast differentiation by means of the gain- or loss-of-function method. We used alkaline phosphatase (ALP) and alizarin red S staining and quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) to determine whether the forced transient expression or knock-down of Twist1 in a mouse cementoblast

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Huang, Bin, Mengyao Zhao, Mingzhe Yang, et al. "Osmanthus Fragrans Loaded NIPAAM Hydrogel Promotes Osteogenic Differentiation of MC3T3-E1." Gels 8, no. 10 (2022): 659. http://dx.doi.org/10.3390/gels8100659.

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There is an urgent need to find long-acting, natural osteogenesis-promoting drug systems. In this study, first the potential targets and mechanism of osmanthus fragrans (O. fragrans) extract in regulating osteogenic differentiation based on autophagy were analyzed by network pharmacology and molecular docking. Then, osmanthus fragrans was extracted using the ethanol reflux method and an osmanthus fragrans extract loaded Poly N-isopropylacrylamide (OF/NIPAAM) hydrogel was prepared by electron beam radiation. The chemical components of the osmanthus fragrans extract and the microstructure of OF/

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Salasznyk, Roman M., William A. Williams, Adele Boskey, Anna Batorsky, and George E. Plopper. "Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells." Journal of Biomedicine and Biotechnology 2004, no. 1 (2004): 24–34. http://dx.doi.org/10.1155/s1110724304306017.

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The mechanisms controlling human mesenchymal stem cells (hMSC) differentiation are not entirely understood. We hypothesized that the contact with extracellular matrix (ECM) proteins normally found in bone marrow would promote osteogenic differentiation of hMSC in vitro. To test this hypothesis, we cultured hMSC on purified ECM proteins in the presence or absence of soluble osteogenic supplements, and assayed for the presence of well-established differentiation markers (production of mineralized matrix, osteopontin, osteocalcin, collagen I, and alkaline phosphatase expression) over a 16-day tim

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Moursi, A. M., R. K. Globus, and C. H. Damsky. "Interactions between integrin receptors and fibronectin are required for calvarial osteoblast differentiation in vitro." Journal of Cell Science 110, no. 18 (1997): 2187–96. http://dx.doi.org/10.1242/jcs.110.18.2187.

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We previously showed that anti-fibronectin antibodies or soluble fibronectin fragments containing the central cell-binding domain inhibit formation of mineralized nodules by fetal calvarial osteoblasts in vitro. These findings suggest a critical role for fibronectin in osteoblast differentiation and morphogenesis. In this study we tested the hypothesis that fibronectin's effects on osteogenesis are mediated via direct interactions with integrin receptors for fibronectin on osteoblasts. Immunocytochemical analysis identified the integrin fibronectin receptor alpha5ss1 in fetal rat calvarial tis

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Bae, Sejin, Bueonguk Kang, Hyungbin Lee, Harrison Luu, Eric Mullins, and Karl Kingsley. "Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates." Journal of Functional Biomaterials 12, no. 1 (2021): 15. http://dx.doi.org/10.3390/jfb12010015.

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Introduction: Many studies in stem cell biology have demonstrated that dental pulp stem cells (DPSC) may be highly proliferative and capable of pluripotent differentiation into many different tissue types. Recent advances in stem cell research have outlined methods for directing in vitro or in vivo growth, viability, and proliferation, as well as differentiation of DPSC—although much remains to be discovered. Based upon this information, the primary objective of this study was to understand the functional biomaterials needed to more effectively direct DPSC viability, growth, and proliferation.

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Hagiwara, H., Y. Hiruma, A. Inoue, A. Yamaguchi, and S. Hirose. "Deceleration by angiotensin II of the differentiation and bone formation of rat calvarial osteoblastic cells." Journal of Endocrinology 156, no. 3 (1998): 543–50. http://dx.doi.org/10.1677/joe.0.1560543.

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We examined the effects of angiotensin II (Ang II) on the differentiation of rat calvarial osteoblastic cells and on the formation of bone by these cells. Northern blotting analysis revealed that Ang II inhibited the expression of mRNA for osteocalcin, which is a protein that is specifically expressed during maturation of osteoblastic cells. Ang II decreased the activity of alkaline phosphatase, a marker of osteoblastic differentiation, in the cells, acting via the type 1 (AT1) receptor. We used von Kossa staining to examine the formation of mineralized nodules by osteoblastic cells. Both the

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Oliveira, K. P., A. M. S. Reis, A. P. Silva, et al. "Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, no. 6 (2017): 1573–80. http://dx.doi.org/10.1590/1678-4162-9364.

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ABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline ph

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Lee, Jin-Sun, Hong Jae Lee, Jae Won Lee, Sang Cheon Lee, and Jung Sun Heo. "Osteogenic Effect of Inducible Nitric Oxide Synthase (iNOS)-Loaded Mineralized Nanoparticles on Embryonic Stem Cells." Cellular Physiology and Biochemistry 51, no. 2 (2018): 746–62. http://dx.doi.org/10.1159/000495330.

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Background/Aims: This study investigated the effect of inducible nitric oxide synthase-loaded mineralized nanoparticles (iNOS-MNPs) on the osteogenic differentiation of mouse embryonic stem cells (ESCs). Methods: We prepared iNOS-MNPs using an anionic block copolymer template-mediated calcium carbonate (CaCO3) mineralization process in the presence of iNOS. iNOS-MNPs were spherical and had a narrow size distribution. iNOS was stably loaded within MNPs without denaturation. In order to confirm the successful introduction of iNOS-MNPs into the cytosol of ESCs, intracellular levels of nitric oxid

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Burgess, Sarah Asha, Tracy D. Branam, and Lee J. Florea. "Divergent Geochemical Pathways of Carbonate Aquifer Evolution in a Classic Karst Terrain: (2) Groundwater Source Delineation Using Regional Water Chemistry Data." Water 15, no. 19 (2023): 3436. http://dx.doi.org/10.3390/w15193436.

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Springs provide ideal monitoring points for groundwater chemistry, which are important for managing groundwater resources. The chemistry of these spring waters aggregate geochemical reactions along the flow path. In this paper, part two of a two-part investigation, 104 perennial springs in the classic karst landscape of the Mitchell Plateau, Indiana, USA were sampled at base flow. Many of these springs are historically important for domestic, agricultural, commercial, and recreational use. Multifactor analysis of field measurements, principal ions, and stable isotopes revealed five primary clu

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Sun, Weiwei, Wenyu Xie, Kun Hu, et al. "Three-Dimensional Bioprinting of Strontium-Modified Controlled Assembly of Collagen Polylactic Acid Composite Scaffold for Bone Repair." Polymers 16, no. 4 (2024): 498. http://dx.doi.org/10.3390/polym16040498.

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In recent years, the incidence of bone defects has been increasing year by year. Bone transplantation has become the most needed surgery after a blood transfusion and shows a rising trend. Three-dimensional-printed implants can be arbitrarily shaped according to the defects of tissues and organs to achieve perfect morphological repair, opening a new way for non-traumatic repair and functional reconstruction. In this paper, strontium-doped mineralized collagen was first prepared by an in vitro biomimetic mineralization method and then polylactic acid was homogeneously blended with the mineraliz

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Van Vlasselaer, P., B. Borremans, U. van Gorp, JR Dasch, and R. De Waal-Malefyt. "Interleukin 10 inhibits transforming growth factor-beta (TGF-beta) synthesis required for osteogenic commitment of mouse bone marrow cells." Journal of Cell Biology 124, no. 4 (1994): 569–77. http://dx.doi.org/10.1083/jcb.124.4.569.

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Interleukin 10 (IL-10) suppressed TGF-beta synthesis in mouse bone marrow cultures. Coincidingly, IL-10 down-regulated the production of bone proteins including alkaline phosphatase (ALP), collagen and osteocalcin, and the formation of mineralized extracellular matrix. The mAb 1D11.16 which neutralizes TGF-beta 1 and TGF-beta 2, induced suppressive effects comparable to IL-10 when administered before the increase of cell proliferation in the culture. It appears that mainly TGF-beta 1 plays a role in this system since (a) TGF-beta 2 levels were undetectable in supernatants from osteogenic cultu

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Rahman, Saeed Ur, Sasikumar Ponnusamy, Malvika Nagrath, and Praveen R. Arany. "Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues." Journal of Tissue Engineering 13 (January 2022): 204173142110739. http://dx.doi.org/10.1177/20417314211073934.

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The major difference between tissue healing and regeneration is the extent of instructional cues available to precisely direct the biological response. A classic example is reparative or osteodentin that is seen in response to physicochemical injury to the pulp-dentin complex. Dentin regeneration can direct the differentiation of dental stem cells using concerted actions of both soluble (biomolecules, agonists, and antagonists) and insoluble (matrix topology) cues. The major purpose of this study was to examine the synergistic combination of two discrete biomaterial approaches by utilizing nan

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AlMuraikhi, Nihal, Dalia Ali, Radhakrishnan Vishnubalaji, et al. "Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells." Stem Cells International 2019 (August 22, 2019): 1–12. http://dx.doi.org/10.1155/2019/3041262.

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Background. Chemical biology approaches using small molecule inhibitors targeting specific signaling pathways are useful tools to dissect the molecular mechanisms governing stem cell differentiation and for their possible use in therapeutic interventions. Methods. Stem cell signaling small molecule library functional screen was performed employing human bone marrow skeletal (mesenchymal) stem cells (hBMSCs). Alkaline phosphatase (ALP) activity and formation of mineralized matrix visualized by Alizarin red staining were employed as markers for osteoblastic differentiation. Global gene expressio

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