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Journal articles on the topic 'Mini-barcodes'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Yeo, Darren, Amrita Srivathsan, and Rudolf Meier. "Longer is Not Always Better: Optimizing Barcode Length for Large-Scale Species Discovery and Identification." Systematic Biology 69, no. 5 (2020): 999–1015. http://dx.doi.org/10.1093/sysbio/syaa014.

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Abstract New techniques for the species-level sorting of millions of specimens are needed in order to accelerate species discovery, determine how many species live on earth, and develop efficient biomonitoring techniques. These sorting methods should be reliable, scalable, and cost-effective, as well as being largely insensitive to low-quality genomic DNA, given that this is usually all that can be obtained from museum specimens. Mini-barcodes seem to satisfy these criteria, but it is unclear how well they perform for species-level sorting when compared with full-length barcodes. This is here

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Xie, Xuena, Haoting Ye, Xuan Cai, et al. "DNA Mini-Barcodes, a Potential Weapon for Conservation and Combating Illegal Trade of Pangolin." Tropical Conservation Science 14 (January 2021): 194008292110173. http://dx.doi.org/10.1177/19400829211017361.

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Background Smuggling and illegal trade of pangolins and their scales has drastically reduced the wild population of pangolins. Accurate species identification is currently in urgent need as a powerful weapon for combating pangolin smuggling and trade and conserving the already endangered pangolin species. Aim of the study To develop an efficient method based on DNA mini-barcodes for accurate pangolin species identification and authentication of processed pangolin scales against the non-target species. Materials and methods The primers for amplifying the DNA mini-barcodes were designed based on

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Goodman, Aaron M., Lorenzo Prendini, and Lauren A. Esposito. "Systematics of the Arboreal Neotropical ‘thorellii’ Clade of Centruroides Bark Scorpions (Buthidae) and the Efficacy of Mini-Barcodes for Museum Specimens." Diversity 13, no. 9 (2021): 441. http://dx.doi.org/10.3390/d13090441.

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Fragmented and degraded DNA is pervasive among museum specimens, hindering molecular phylogenetics and species identification. Mini-barcodes, 200–300-base-pair (bp) fragments of barcoding genes, have proven effective for species-level identification of specimens from which complete barcodes cannot be obtained in many groups, but have yet to be tested in arachnids. The present study investigated the efficacy of mini-barcodes combined with longer sequences of the Cytochrome c Oxidase Subunit I (COI) gene in the systematics of the arboreal Neotropical ‘thorellii’ clade of Centruroides Marx, 1890

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Su'udi, Mukhamad, Zakiyah Ramadany, Siti Rohimah, Asyifa Yasmin Arum, Dwi Setyati, and Fuad Bahrul Ulum. "Peran Mini-Barcode Internal Transcribed Spacer 2 (ITS2) untuk Identifikasi Molekuler Spesies Anggrek." Indonesian Journal of Biotechnology and Biodiversity 7, no. 1 (2023): 34–45. http://dx.doi.org/10.47007/ijobb.v7i1.166.

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AbstractMolecular identification using short orthological DNA sequences (DNA barcoding) has been applied for the classification of orchid species which is a major step in biodiversity management, conservation, breeding, authenticating components of herbal products, and tracking the adulteration of orchid species. One of the most widely used loci for phylogenetic inference at the generic and infrageneric levels in plants was ITS located between 18S rDNA, 5.8S rDNA, and 26S rDNA. The ITS or ITS2 region has been suggested as a plant barcode in some previous studies. However, DNA barcoding using t

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Abdullah, Asadatun, Mala Nurilmala, and Kinanti Permata Sitaresmi. "DNA Mini-Barcodes as A Molecular Marker for Various Hairtail Fish Products Traceability." Jurnal Pengolahan Hasil Perikanan Indonesia 22, no. 1 (2019): 33. http://dx.doi.org/10.17844/jphpi.v22i1.25874.

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Hairtail fishery is an important fishery commodity with high economic value as export commodity. Authentication based on DNA mini-barcodes can be applied to overcome seafood fraud and to support Indonesian traceability system. This study was aimed to design species-specific primers of DNA minibarcodes and authentication of various processed hairtail fish products such as smoked, baked, fried, canned, and boiled. Analytical method applied PCR-based of cytochrome c oxidase 1 (COI) full length and<br />mini-barcodes. Mini-barcode primer specific for hairtail (Trichiurus lepturus) successful

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VAN HOUDT, J. K. J., F. C. BREMAN, M. VIRGILIO, and M. DE MEYER. "Recovering full DNA barcodes from natural history collections of Tephritid fruitflies (Tephritidae, Diptera) using mini barcodes." Molecular Ecology Resources 10, no. 3 (2010): 459–65. http://dx.doi.org/10.1111/j.1755-0998.2009.02800.x.

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Abdullah, Asadatun, Mala Nurilmala, Aulia Sekar Sari, and Agoes Mardiono Jacoeb. "MINI-COI BARCODES SEBAGAI PENANDA MOLEKULER UNTUK KETERTELUSURAN LABEL PANGAN BERBAGAI PRODUK OLAHAN IKAN SIDAT." Jurnal Pengolahan Hasil Perikanan Indonesia 21, no. 2 (2018): 377. http://dx.doi.org/10.17844/jphpi.v21i2.23507.

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Ikan Sidat merupakan komoditas hasil perikanan penting dengan nilai ekonomis tinggi. DNA barcoding telah cukup banyak digunakan sebagai metode berbasis penentuan urutan nukelotida untuk mendeteksi jenis spesies pada ikan. Proses pengolahan dengan suhu tinggi dapat menyulitkan proses identifikasi berbasis penentuan urutan nukelotida. Tujuan penelitian ini adalah untuk mengidentifikasi spesies berbagai produk olahan ikan sidat menggunakan marka molekuler <em>mini-COI barcodes.</em> Pengolahan suhu tinggi yang digunakan pada penelitian ini adalah: panggang, penggorengan dalam minyak s

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Boza, Beatriz R., Vanessa P. Cruz, Gustavo Stabile, Matheus M. Rotundo, Fausto Foresti, and Claudio Oliveira. "Mini DNA barcodes reveal the details of the foraging ecology of the largehead hairtail, Trichiurus lepturus (Scombriformes: Trichiuridae), from São Paulo, Brazil." Neotropical Ichthyology 20, no. 2 (2022): 1–11. https://doi.org/10.1590/1982-0224-2021-0166.

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Boza, Beatriz R., Cruz, Vanessa P., Stabile, Gustavo, Rotundo, Matheus M., Foresti, Fausto, Oliveira, Claudio (2022): Mini DNA barcodes reveal the details of the foraging ecology of the largehead hairtail, Trichiurus lepturus (Scombriformes: Trichiuridae), from São Paulo, Brazil. Neotropical Ichthyology (e210166) 20 (2): 1-11, DOI: 10.1590/1982-0224-2021-0166, URL: http://dx.doi.org/10.1590/1982-0224-2021-0166

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Chen, Mingyang, Xuan Han, Jiao Ma, et al. "Identification of Plastrum Testudinis used in traditional medicine with DNA mini-barcodes." Revista Brasileira de Farmacognosia 28, no. 3 (2018): 267–72. http://dx.doi.org/10.1016/j.bjp.2018.04.008.

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Abdullah, Asadatun, Ari Elisa Ratih, Shabrina Aulia, Puji Rianti, Tati Nurhayati, and Agoes Mardiono Jacoeb. "Autentikasi Produk Olahan Ikan Hiu Komersial menggunakan Teknik Species-Specific DNA Mini-barcodes." Jurnal Pengolahan Hasil Perikanan Indonesia 23, no. 2 (2020): 383–91. http://dx.doi.org/10.17844/jphpi.v23i2.32226.

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Pemanfaatan ikan hiu sebagai bahan baku produk olahan perikanan dapat menyebabkan berkurangnya populasi ikan hiu yang juga rentan akan kepunahan. Ikan hiu pada umumnya diperdagangkan dalam bentuk sirip, daging ikan yang diasinkan dan direbus, minyak ikan serta sebagai bahan subtitusi pada pakan hewan sehingga proses identifikasi menggunakan metode berbasis morfologi dan meristik sulit dilakukan. Sebagai alternatif proses autentikasi bahan baku produk olahan ikan hiu adalah menggunakan teknik DNA mini barcodes. Penelitian ini bertujuan untuk merancang dan mengaplikasikan primer DNA mini barcode

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Parveen, Iffat, Natascha Techen, and Ikhlas Khan. "Identification of Species in the Aromatic Spice Family Apiaceae Using DNA Mini-barcodes." Planta Medica 85, no. 02 (2018): 139–44. http://dx.doi.org/10.1055/a-0664-0947.

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AbstractThe species of the aromatic plant family Apiaceae are mainly used as spices and foods, but the family also includes medicinal and some poisonous plant species. Due to the similar chemical compounds or aroma and morphology, the poisonous species are often mistaken for the edible aromatic species. It is therefore imperative to correctly identify the species present at the initial raw stage samples to ensure product safety and efficacy. At the molecular level, plant species can be identified using DNA loci either from nuclear or plastid genome with easily available universal oligonucleoti

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Villa, Caterina, Joana Costa, Liliana Meira, M. Beatriz P. P. Oliveira, and Isabel Mafra. "Exploiting DNA mini-barcodes as molecular markers to authenticate saffron (Crocus sativus L.)." Food Control 65 (July 2016): 21–31. http://dx.doi.org/10.1016/j.foodcont.2016.01.008.

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Abdullah, Asadatun, Hana Aulia Sativa, Tati Nurhayati, and Mala Nurilmala. "DNA Barcoding Application for Seafood Label Traceability of Various Commercial Surimi-Based Products." Jurnal Pengolahan Hasil Perikanan Indonesia 22, no. 3 (2019): 508–19. http://dx.doi.org/10.17844/jphpi.v22i3.28950.

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Surimi-based processed products are prone to mislabeling using raw materials that are not in accordance with food safety requirements. There were cases reporting use of toxic fish tissue in commercial seafood products. This study was aimed to identify and determine the raw materials used in various processed surimi products using cytochrome oxidase I (COI) gene marker. The experiment consisted of DNA isolation, DNA amplification using several target primers namely full-length barcodes, mini-barcodes, as well as specific species primers against poisonous puffer fish Lagocephalus lunaris and gen

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Marquisseau, Anaïs, Kamila Canale-Tabet, Emmanuelle Labarthe, et al. "Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France." Biodiversity Data Journal 13 (January 7, 2025): e137540. https://doi.org/10.3897/BDJ.12.e137540.

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DNA barcoding and metabarcoding are now powerful tools for studying biodiversity and especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on the taxonomic resolution of the DNA sequences used as barcodes and on the reliability of the reference databases. For wild bees, the barcode sequences coverage is consistently growing in volume, but some incorrect species annotations need to be cared for. The COI (Cytochrome Oxydase subunit 1) gene, the most used in barcoding/metabarcoding of arthropods, suffers from primer

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Alberdi, Antton, Inazio Garin, Ostaizka Aizpurua, Joxerra Aihartza, and Brock Fenton. "The Foraging Ecology of the Mountain Long-Eared Bat Plecotus macrobullaris Revealed with DNA Mini-Barcodes." PLoS ONE 7, no. 4 (2012): e35692. https://doi.org/10.5281/zenodo.14821329.

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(Uploaded by Plazi for the Bat Literature Project) Molecular analysis of diet overcomes the considerable limitations of traditional techniques for identifying prey remains in bat faeces. We collected faeces from individual Mountain Long-eared Bats Plecotus macrobullaris trapped using mist nets during the summers of 2009 and 2010 in the Pyrenees. We analysed their diet using DNA mini-barcodes to identify prey species. In addition, we inferred some basic features of the bat's foraging ecology that had not yet been addressed. P. macrobullaris fed almost exclusively on moths (97.8%). As prey we de

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Gu, Hai-Feng, Yun Xia, Rui Peng, Bang-Hui Mo, Li Li, and Xiao-Mao Zeng. "Authentication of Chinese Crude Drug Gecko by DNA Barcoding." Natural Product Communications 6, no. 1 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600117.

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Gekko gecko, an animal used as a valued traditional Chinese medicine, has been widely used for over 2000 years. Due to localized habitat destruction, the amount of G. gecko has dramatically decreased in recent years. As a result, more and more adulterants have been detected in the traditional medicine, which has resulted in a chaotic market. Therefore, a correct identification method is badly needed. In this study, we employed a new molecular method of DNA barcoding for discriminating gecko from its adulterants. Fifty-seven specimens of gecko and its adulterants were collected as test samples.

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Zhou, Qian-Ru, Yun-Ying Ma, Hu-Qiang Lv, et al. "Chloroplast mini-barcodes combined with high resolution melting analysis to identify herbal medicine Difengpi (Illicium difengpi)." Heliyon 10, no. 19 (2024): e38700. http://dx.doi.org/10.1016/j.heliyon.2024.e38700.

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SRIRAMA, R., B. R. GURUMURTHY, U. SENTHILKUMAR, G. RAVIKANTH, R. UMA SHAANKER, and M. B. SHIVANNA. "Are mini DNA-barcodes sufficiently informative to resolve species identities? An in silico analysis using Phyllanthus." Journal of Genetics 93, no. 3 (2014): 823–29. http://dx.doi.org/10.1007/s12041-014-0432-6.

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Alberdi, Antton, Inazio Garin, Ostaizka Aizpurua, and Joxerra Aihartza. "The Foraging Ecology of the Mountain Long-Eared Bat Plecotus macrobullaris Revealed with DNA Mini-Barcodes." PLoS ONE 7, no. 4 (2012): e35692. http://dx.doi.org/10.1371/journal.pone.0035692.

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Costa, Nelice, Eduarda Gianni, Rômulo Salignac Araújo de Faria, and Carlos Benigno Vieira de Carvalho. "DNA barcoding e mini-barcoding na rotina forense: avaliação da viabilidade e da eficácia das técnicas na identificação de espécies animais em amostras degradadas." Revista Brasileira de Criminalística 13, no. 2 (2024): 83–91. http://dx.doi.org/10.15260/rbc.v13i2.751.

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Apesar de os protocolos e técnicas de identificação genética de espécies animais terem avançado muito rapidamente no meio acadêmico, sua aplicação pelos órgãos oficiais de criminalística brasileiros ainda é restrita. A fim de avaliar a viabilidade e a eficácia das técnicas de DNA barcoding e mini-barcoding na identificação genética de espécies animais no contexto forense, 23 amostras antigas, degradadas ou armazenadas de forma não ideal, de identidade conhecida, foram selecionadas para este estudo. As etapas de extração do DNA, amplificação e sequenciamento foram realizadas de forma linear e s

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Lee, Shiou Yih, Dhilia Udie Lamasudin, and Rozi Mohamed. "Rapid detection of several endangered agarwood-producing Aquilaria species and their potential adulterants using plant DNA barcodes coupled with high-resolution melting (Bar-HRM) analysis." Holzforschung 73, no. 5 (2019): 435–44. http://dx.doi.org/10.1515/hf-2018-0149.

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Abstract Aquilaria is an endangered agarwood-producing genus that is currently protected by international laws. The agarwood trade is strictly monitored to prevent illegal harvesting, which has caused high demand for this natural product. Other plant sources of similar appearance or fragrance as agarwood are used as adulterant species in counterfeit products. To promote species identification via the DNA barcoding technique, the existing DNA barcoding database in our laboratory was enriched with seven plant barcoding sequences from a commercially important Aquilaria species (Aquilaria beccaria

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Suudi, Muhammad. "The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids." Life Science and Biotechnology 1, no. 1 (2023): 21. http://dx.doi.org/10.19184/lsb.v1i1.39851.

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matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets tha

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SZPILA, KRZYSZTOF, DOMINIKA WYBORSKA, CEZARY BYSTROWSKI, and THOMAS PAPE. "Redescription of Sphecapatoclea excisa Villeneuve, 1909 (Diptera: Sarcophagidae)." Zootaxa 4728, no. 1 (2020): 110–22. http://dx.doi.org/10.11646/zootaxa.4728.1.5.

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Sphecapatoclea excisa Villeneuve, 1909, the type species of the Palaearctic genus Sphecapatoclea Villeneuve, 1909, is redescribed based on a female syntype and on material from Makhtesh Ramon National Park, Israel, and its first instar larva is described for the first time. The species is sexually dimorphic, with much darker adult males. The male genital apparatus is unique by its compressed and sclerotised epiphallus. The morphology of the first instar larva is in accordance with the recently suggested position of the genus Sphecapatoclea in the Old World clade of the “lower” Miltogramminae.

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Mkenda, Ndakidemi, Stevenson, et al. "Characterization of Hymenopteran Parasitoids of Aphis fabae in an African Smallholder Bean Farming System through Sequencing of COI ‘Mini-Barcodes’." Insects 10, no. 10 (2019): 331. http://dx.doi.org/10.3390/insects10100331.

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Parasitoids are among the most frequently reported natural enemies of insect pests, particularly aphids. The efficacy of parasitoids as biocontrol agents is influenced by biotic and abiotic factors. For example, hyperparasitoids can reduce the abundance of the primary parasitoids as well as modify their behavior. A field study was conducted at three contrasting elevations on Mount Kilimanjaro, Tanzania, to identify the parasitoids of aphids in smallholder bean farming agroecosystems. Sentinel aphids (Aphis fabae) on potted bean plants (Phaseolus vulgaris) were exposed in 15 bean fields at thre

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Boyer, Stephane, Samuel D. J. Brown, Rupert A. Collins, et al. "Sliding Window Analyses for Optimal Selection of Mini-Barcodes, and Application to 454-Pyrosequencing for Specimen Identification from Degraded DNA." PLoS ONE 7, no. 5 (2012): e38215. http://dx.doi.org/10.1371/journal.pone.0038215.

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Velasco-Cuervo, Sandra M., Elkin Aguirre-Ramirez, Jenny Johana Gallo-Franco, Ranulfo González Obando, Nancy Carrejo, and Nelson Toro-Perea. "Saving DNA from museum specimens: The success of DNA mini-barcodes in haplotype reconstruction in the genus Anastrepha (Diptera: Tephritidae)." Journal of Advanced Research 16 (March 2019): 123–34. http://dx.doi.org/10.1016/j.jare.2018.11.001.

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Buddhachat, Kittisak, Chadaporn Attakitbancha, Onchira Ritbamrung, Kanmethar Chanthap, Chatmongkon Suwannapoom, and Korakot Nganvongpanit. "Using mini-barcodes coupled with high resolution melting (minibar-HRM) method for species discrimination across Pangasianodon gigas, Pangasianodon hypophthalmus and Pangasius larnaudii." Aquaculture 530 (January 2021): 735773. http://dx.doi.org/10.1016/j.aquaculture.2020.735773.

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Lees, David C., Rodolphe Rougerie, Christof Zeller-Lukashort, and Niels P. Kristensen. "DNA mini-barcodes in taxonomic assignment: a morphologically unique new homoneurous moth clade from the Indian Himalayas described in Micropterix (Lepidoptera, Micropterigidae)." Zoologica Scripta 39, no. 6 (2010): 642–61. http://dx.doi.org/10.1111/j.1463-6409.2010.00447.x.

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Falk, Bryan G., and Robert N. Reed. "Challenges to a molecular approach to prey identification in the Burmese python,Python molurus bivittatus." PeerJ 3 (November 24, 2015): e1445. http://dx.doi.org/10.7717/peerj.1445.

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Molecular approaches to prey identification are increasingly useful in elucidating predator–prey relationships, and we aimed to investigate the feasibility of these methods to document the species identities of prey consumed by invasive Burmese pythons in Florida. We were particularly interested in the diet of young snakes, because visual identification of prey from this size class has proven difficult. We successfully extracted DNA from the gastrointestinal contents of 43 young pythons, as well as from several control samples, and attempted amplification of DNA mini-barcodes, a 130-bp region

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Bin Mulyadi, Yahya, Hasan, Al Muadza Zainuddin, Isminarti, and Muhammad Nur. "Implementation of AGV Robot Technology for the Goods Distribution Process at the Bosowa Polytechnic Teaching Factory." Jurnal Fokus Elektroda : Energi Listrik, Telekomunikasi, Komputer, Elektronika dan Kendali) 9, no. 4 (2024): 239–42. https://doi.org/10.33772/jfe.v9i4.197.

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In the modern industrial era, increasing operational efficiency and automation have become top priorities in various sectors, including manufacturing, logistics, and distribution. In this context, Robot Automated Guided Vehicle (AGV) has become a highly sought-after solution to meet the increasing demands for efficiency and productivity. This study aims to implement Robot AGV technology in the distribution process of goods at the Bosowa Polytechnic Teaching Factory. This AGV robot is designed to move independently using a Pixy camera sensor that can read barcodes as a determinant of the direct

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Herman, Herman, Mayang Sari, Nella Multivasari, and Dewi Indriyani Roslim. "DNA Barcoding Analysis Kitolod (Hippobroma longiflora) from Riau Based on matk Gene." Jurnal Biologi Tropis 25, no. 1 (2025): 653–59. https://doi.org/10.29303/jbt.v25i1.8479.

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The kitolod plant (Hippobroma longiflora) is a traditional medicinal plant originating from the Campanulaceae family. DNA barcoding is a technique for identifying an organism using short nucleotide sequences known as DNA barcoding. One of the DNA barcodes in plants is matK. This research aims to analyze DNA barcode sequence in the matK region of the kitolod plant using the DNA barcode. Samples were taken from the area of Tarai Bangun Village, Kampar Regency, Riau Province, Indonesia as many as two different individuals. The research stages carried out are sampling, followed by DNA isolation us

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Chua, Physilia, Petra Korlevic, Lyndall Pereira-da-Conceicoa, Cameron Ferguson, Leia Zhao, and Mara Lawniczak. "Let's talk about the (lady)birds and the bees: how insects can whisper a multitude of stories." ARPHA Conference Abstracts 5 (June 17, 2022): e85529. https://doi.org/10.3897/aca.5.e85529.

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If you have watched <em>A Bug's Life</em>, you would have seen that insects come in an assortment of colours, shapes, and sizes. They are the perfect organism that could be used to describe the myriad diversity of all life on earth. These six-legged creatures are one of the most diverse groups of species, accounting for more than 80% of all documented living animals Ødegaard 2008. This huge diversity also makes it extremely time- and labour-intensive to carry out large-scale monitoring of insects. High-throughput sequencing technologies and the use of DNA barcodes for species identification ha

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Cárdenas, Paco, and Jon A. Moore. "First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens." Marine Biodiversity 49, no. 1 (2017): 163–74. http://dx.doi.org/10.1007/s12526-017-0775-3.

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Costa, Joana, Bruna Campos, Joana S. Amaral, M. Eugénia Nunes, M. Beatriz P. P. Oliveira, and Isabel Mafra. "HRM analysis targeting ITS1 and matK loci as potential DNA mini-barcodes for the authentication of Hypericum perforatum and Hypericum androsaemum in herbal infusions." Food Control 61 (March 2016): 105–14. http://dx.doi.org/10.1016/j.foodcont.2015.09.035.

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Gilbert, Ocen, Semwogerere Twaibu, Sekamwa Umar, and Matovu Davis. "An Analytical Review of Existing Supermarket Billing Systems." East African Journal of Information Technology 4, no. 1 (2021): 1–6. http://dx.doi.org/10.37284/eajit.4.1.440.

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Retail shops and stores form part of the nation’s business sector involved in selling and providing several goods and services. The main objective was to study and analyse existing supermarket billing systems and possibly identify their weaknesses and strengths. This will enable us to generate an RFID billing system that is unique and even more advantageous than the existing systems. The methodology used was qualitative involving hard and soft document reviews and observational experiments. Findings revealed that there are several billing systems which include the manual price tag system where

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Sawicki, Jakub, Katarzyna Krawczyk, Monika Ślipiko, Kamil Szandar, and Monika Szczecińska. "Comparative Analysis of Apopellia endiviifolia Plastomes Reveals a Strikingly High Level of Differentiation between Its Terrestrial and Water Form." Diversity 13, no. 12 (2021): 674. http://dx.doi.org/10.3390/d13120674.

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The simple thalloid liverwort Apopellia endiviifolia is a widespread Holarctic species belonging to the family Pelliaceae. European populations of this species comprise two distinct evolutionary lineages named “species A”, known also as water form, and typical, mainly terrestrial forms named “species B”. Newly sequenced, assembled and annotated chloroplast genomes of six European specimens belonging to the two cryptic lineages occupying different microhabitats, revealed the structure typical for liverworts and previously sequenced reference. The plastomes of A. endiviifolia are 120,537–120,947

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Lancioni, Giulio E., Gloria Alberti, Chiara Filippini, et al. "A New Technology System to Support Occupational Activity and Mobility in People with Severe-to-Profound Intellectual Disability and Blindness." OBM Neurobiology 09, no. 01 (2025): 1–20. https://doi.org/10.21926/obm.neurobiol.2501268.

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People with severe-to-profound intellectual disability and blindness tend to be sedentary and detached, given their orientation and mobility problems, often combined with their limited interest in their surroundings. An approach to address this problem may involve the use of technology systems guiding the participants through mobility and occupational activity (object use) with a combination of spatial/orientation cues, basic instructions for the responses required by the activity, and positive stimulation during the activity. This study extended the above-mentioned approach using a new techno

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Long, Dang Thanh, Hoang Thi Kim Hong, and Le Ly Thuy Tram. "Analysis genetic variation and phylogenetic relationships of local lotus collected in Thua Thien hue province, Vietnam by DNA barcoding." Research Journal of Biotechnology 17, no. 2 (2022): 48–56. http://dx.doi.org/10.25303/1702rjbt4856.

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Universal conventional DNA barcodes will become more and more popular in plant species using short sequences of chloroplast DNA identifications. DNA mini-barcode is a solution for such specific purposes. Here we exemplify how to develop the best minibarcodes for specific taxa using the Nelumbo nucifera as an example. Six chloroplast DNA regions (accDpsaI, ndhA, psbE-petL, Rpl32-trnL, trnW – psaJ, trnSGCU-trnGGCC) were tested for their suitability as DNA barcoding regions of thirty three lotus samples (white and pink) which were collected in Thua Thien Hue province, Vietnam. Universal primers w

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Xu, Jianping. "Fungal DNA barcoding." Genome 59, no. 11 (2016): 913–32. http://dx.doi.org/10.1139/gen-2016-0046.

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Fungi are ubiquitous in both natural and human-made environments. They play important roles in the health of plants, animals, and humans, and in broad ecosystem functions. Thus, having an efficient species-level identification system could significantly enhance our ability to treat fungal diseases and to monitor the spatial and temporal patterns of fungal distributions and migrations. DNA barcoding is a potent approach for rapid identification of fungal specimens, generating novel species hypothesis, and guiding biodiversity and ecological studies. In this mini-review, I briefly summarize (i)

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Wu, Sophie R., Julianne Sharpe, Joshua Tolliver, et al. "Abstract 5156: Combining the RCAS/tv-a retrovirus and CRISPR/Cas9 gene editing systems to generate primary mouse models of midline glioma." Cancer Research 85, no. 8_Supplement_1 (2025): 5156. https://doi.org/10.1158/1538-7445.am2025-5156.

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Abstract Diffuse midline gliomas (DMGs) are fatal pediatric brain tumors that commonly arise in the brainstem and other midline brain structures, with a median overall survival of less than two years. Genetically engineered mouse models (GEMMs) are key to studying tumorigenesis and tumor-immune interactions, which may inform the development of urgently-needed treatment approaches. However, current midline glioma GEMM approaches are limited in their ability to model multiple gene perturbations and to target specific cell lineages in the brain. Here, we establish a platform that combines the RCA

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Motamedinia, Behnam, Jeffrey H. Skevington, and Scott Kelso. "Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes." ZooKeys 873 (August 29, 2019): 85–111. http://dx.doi.org/10.3897/zookeys.873.36645.

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The Middle East species of Claraeola Aczél (Diptera, Pipunculidae) are revised based on morphological characteristics and sequence data from the mitochondrial COI barcoding gene, using a novel COI mini-barcode protocol. Four new Claraeola species are described: C. bousynterga Motamedinia &amp; Skevington, sp. nov., C. heidiae Motamedinia &amp; Skevington, sp. nov., C. khuzestanensis Motamedinia &amp; Skevington, sp. nov., and C. mantisphalliga Motamedinia &amp; Skevington, sp. nov.Eudorylas thekkadiensis Kapoor, Grewal &amp; Sharma, 1987 is transferred to Claraeola, C. thek

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Motamedinia, Behnam, Jeffrey H. Skevington, and Scott Kelso. "Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes." ZooKeys 873 (August 29, 2019): 85–111. https://doi.org/10.3897/zookeys.873.36645.

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The Middle East species of Claraeola Aczél (Diptera, Pipunculidae) are revised based on morphological characteristics and sequence data from the mitochondrial COI barcoding gene, using a novel COI mini-barcode protocol. Four new Claraeola species are described: C. bousynterga Motamedinia & Skevington, sp. nov., C. heidiae Motamedinia & Skevington, sp. nov., C. khuzestanensis Motamedinia & Skevington, sp. nov., and C. mantisphalliga Motamedinia & Skevington, sp. nov. Eudorylas thekkadiensis Kapoor, Grewal & Sharma, 1987 is transferred to Claraeola, C. thekkadiensis (comb. no

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Cárdenas, Paco, and Jon A. Moore. "First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens." Marine Biodiversity, August 25, 2017. https://doi.org/10.1007/s12526-017-0775-3.

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We report the first records of the sponge genus <em>Geodia</em> (Demospongiae, Tetractinellida, Geodiidae) from the New England Seamounts and Muir Seamount, at lower bathyal depths. Nine specimens collected between 2000 and 2005 belong to two boreal species (<em>Geodia macandrewii</em> and <em>Geodia barretti</em>) and a temperate species (<em>Geodia megastrella</em>). These records extend the distributions of these deep-sea amphi-Atlantic species to the west. Most of these specimens were originally fixed in formalin, which substantially degraded the DNA. We nonetheless managed to sequence two

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Milan, David T., Izabela S. Mendes, Júnio S. Damasceno, Daniel F. Teixeira, Naiara G. Sales, and Daniel C. Carvalho. "New 12S metabarcoding primers for enhanced Neotropical freshwater fish biodiversity assessment." Scientific Reports 10, no. 1 (2020). http://dx.doi.org/10.1038/s41598-020-74902-3.

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Abstract The megadiverse Neotropical fish fauna lacks a comprehensive and reliable DNA reference database, which hampers precise species identification and DNA based biodiversity assessment in the region. Here, we developed a mitochondrial 12S ribosomal DNA reference database for 67 fish species, representing 54 genera, 25 families, and six major Neotropical orders. We aimed to develop mini-barcode markers (i.e. amplicons with less than 200 bp) suitable for DNA metabarcoding by evaluating the taxonomic resolution of full-length and mini-barcodes and to determine a threshold value for fish spec

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Yu, Xiaolei, Wei Tan, Han Gao, Lin Miao, and Xiaoxuan Tian. "Development of a Specific Mini-Barcode From Plastome and its Application for Qualitative and Quantitative Identification of Processed Herbal Products Using DNA Metabarcoding Technique: A Case Study on Senna." Frontiers in Pharmacology 11 (December 17, 2020). http://dx.doi.org/10.3389/fphar.2020.585687.

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Herbal products play an important role globally in the pharmaceutical and healthcare industries. However, some specific groups of herbal products are easily adulterated by confused materials on the market, which seriously reduces the products’ quality. Universal conventional DNA barcodes would function poorly since the processed herbal products generally suffer from varying degrees of DNA degradation and DNA mixing during processing or manufacturing. For quality control purposes, an accurate and effective method should be provided for species identification of these herbal products. Here, we p

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Guo, Mali, Chaohai Yuan, Leyan Tao, Yafei Cai, and Wei Zhang. "Life barcoded by DNA barcodes." Conservation Genetics Resources, August 15, 2022. http://dx.doi.org/10.1007/s12686-022-01291-2.

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AbstractThe modern concept of DNA-based barcoding for cataloguing biodiversity was proposed in 2003 by first adopting an approximately 600 bp fragment of the mitochondrial COI gene to compare via nucleotide alignments with known sequences from specimens previously identified by taxonomists. Other standardized regions meeting barcoding criteria then are also evolving as DNA barcodes for fast, reliable and inexpensive assessment of species composition across all forms of life, including animals, plants, fungi, bacteria and other microorganisms. Consequently, global DNA barcoding campaigns have r

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Yang, Xia, Xiaolei Yu, Xiaoying Zhang, et al. "Development of Mini-Barcode Based on Chloroplast Genome and Its Application in Metabarcoding Molecular Identification of Chinese Medicinal Material Radix Paeoniae Rubra (Chishao)." Frontiers in Plant Science 13 (March 31, 2022). http://dx.doi.org/10.3389/fpls.2022.819822.

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Radix Paeoniae Rubra (Chishao), a typical multi-origin Chinese medicinal material, originates from the dried roots of Paeonia lactiflora or P. veitchii. The previous study suggested that these two commonly used Chishao showed variation in their chemical compositions and clinical efficacies. Therefore, accurate identification of different Chishao species was of great significance for the guide of clinical medication, and timely treatment of patients. In this study, the chloroplast genome sequences of P. lactiflora and P. veitchii were obtained by next-generation sequencing (NGS) technology, and

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Ran-Ran, Xing, Hu Ran-Ran, Han Jian-Xun, Deng Ting-Ting, and Chen Ying. "DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market." October 16, 2019. https://doi.org/10.1016/j.foodchem.2019.125653.

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This study used DNA barcoding and DNA mini-barcoding to test a variety of animal-derived food products sold in the Chinese market for potential mislabeling. Samples (52) including meat, poultry, and fish purchased from retail and online sources were examined. Regions of cytochrome <em>C</em> oxidase I (<em>COI</em>) gene (~650 bp) and <em>16S rRNA</em> (~220 bp) were used as full- and mini-barcode markers, respectively. Approximately 94% (49 of 52) of the samples generated barcode sequences. The failure rate for full <em>COI</em> full-barcodes was 44%, but we obta

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Mahima, Karthikeyan, Koppala Narayana Sunil Kumar, Kanakarajan Vijayakumari Rakhesh, Parameswaran Sathiya Rajeswaran, Ashutosh Sharma, and Ramalingam Sathishkumar. "Advancements and future prospective of DNA barcodes in the herbal drug industry." Frontiers in Pharmacology 13 (October 21, 2022). http://dx.doi.org/10.3389/fphar.2022.947512.

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Ethnopharmacological relevance: The past couple of decades have witnessed the global resurgence of medicinal plants in the field of herbal-based health care. Increased consumption of medicinal plants and their derivative products is the major cause of the adulteration issues in herbal industries. As a result, the quality of herbal products is affected by spurious and unauthorized raw materials. Recent development in molecular plant identification using DNA barcodes has become a robust methodology to identify and authenticate the adulterants in herbal samples. Hence, rapid and accurate identifi

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Zhu, Shuang, Qiaozhen Liu, Simin Qiu, Jiangpeng Dai, and Xiaoxia Gao. "DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances." Chinese Medicine 17, no. 1 (2022). http://dx.doi.org/10.1186/s13020-022-00655-y.

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AbstractTraditional Chinese medicine (TCM) plays an important role in the global traditional health systems. However, adulterated and counterfeit TCM is on the rise. DNA barcoding is an effective, rapid, and accurate technique for identifying plant species. In this study, we collected manuscripts on DNA barcoding published in the last decade and summarized the use of this technique in identifying 50 common Chinese herbs listed in the Chinese pharmacopoeia. Based on the dataset of the major seven DNA barcodes of plants in the NCBI database, the strengths and limitations of the barcodes and thei

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