Academic literature on the topic 'Minimal Inhibitory Concentration (MIC) method'
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Journal articles on the topic "Minimal Inhibitory Concentration (MIC) method"
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Surana, Anjali, Chauhan Priya, Akotiya Bhavya, G. Saha Suparna, S. Agarwal Rolly, and Manasi Kewlani. "Comparative evaluation of minimal inhibitory concentration and minimal bactericidal concentration of various herbal irrigants against Enterococcus faecalis." Journal of Conservative Dentistry and Endodontics 27, no. 7 (2024): 780–84. http://dx.doi.org/10.4103/jcde.jcde_349_23.
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Context: The purpose of this article is to evaluate the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Herbal Irrigants. Aim: The aim of the study was to evaluate MIC and MBC of herbal extracts of Azadirachta indica, Curcuma longa, and Green Tea Against Enterococcus faecalis. Methodology: The MIC and MBC of extracts of A . indica (neem), C. longa (turmeric), and Green Tea were evaluated to establish them as standard root canal irrigants against E. faecalis using agar well diffusion method. Statistical Analysis Used: The collected data were statistically analyzed using the Statistical Package for the Social Sciences (SPSS) software. Results: The present study found that green tea exhibited the most substantial antimicrobial activity among the tested herbal extracts, which was comparable to chlorhexidine. Although A. indica and C. longa required higher concentrations for effectiveness, their antimicrobial properties were also apparent. Conclusions: Within the constraints of this study, it can be concluded that green tea could be considered a promising alternative to chlorhexidine in treating endodontic infections due to its substantial antimicrobial activity against E. faecalis at lower concentrations.
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Yetendje Chimi, Larissa, Armel Joseph Agokeng, Guy Sedar Singor Njateng, and Jean Paul Dzoyem. "Effect of combination of doxycycline with natural products against planktonic cells, biofilm, and virulence factor of Pseudomonas aeruginosa." Investigational Medicinal Chemistry and Pharmacology 7, no. 1 (2023): 1–8. http://dx.doi.org/10.31183/imcp.2024.00091.
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Background: Pseudomonas aeruginosa is an important environmental, opportunistic, and nosocomial pathogen with a significant threat to public health. Combination therapy has many advantages due to the simultaneous action of two drugs on two separate cellular targets. In the present study, the effect of the combination of doxycycline and natural products against planktonic cells, biofilm, and virulence factors of P. aeruginosa was evaluated. Methods: To perform this work, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of doxycycline and natural products were determined by broth microdilution method. The microtiter plate assay method was used to determine the minimal biofilm inhibitory concentration (MBIC) and the minimum biofilm eradication concentration (MBEC). The effect of doxycycline and natural products against pyocyanin, swarming motility, and swimming motility was evaluated. The checkerboard method was used to evaluate the effect of the combination of doxycycline with natural products against planktonic and biofilm cells. Results: The MIC of doxycycline ranges between 2 µg/mL and 128 µg/mL with an average of 35.89 µg/mL. Sinapic acid shows the best inhibitory activity against planktonic cells with an average MIC of 27.79 µg/mL. At the sub-inhibitory concentrations, the pyocyanin production, swarming motility, and swimming motility decrease. Out of the six combinations tested, the combination formed by doxycycline and sinapic acid exhibited synergistic activity for the prevention of biofilm formation with a 7-fold reduction in MBIC of doxycycline. Conclusion: This study revealed that the doxycycline and sinapic acid combination could be considered as a promising candidate for the development of therapy against P. aeruginosa infections.
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Heuser, Elisa, Karsten Becker, and Evgeny A. Idelevich. "Bactericidal Activity of Sodium Bituminosulfonate against Staphylococcus aureus." Antibiotics 11, no. 7 (2022): 896. http://dx.doi.org/10.3390/antibiotics11070896.
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Antibiotic resistance is increasing worldwide making it necessary to search for alternative antimicrobials. Sodium bituminosulfonate is a long-known substance, whose antimicrobial inhibitory activity has recently been re-evaluated. However, to the best of our knowledge, the bactericidal mode of action of this substance has not been systematically characterized. The aim of this study was to investigate the in vitro bactericidal activity of sodium bituminosulfonate by determining the minimal bactericidal concentrations (MBC), as well as the rapidity of bactericidal effect by time-kill curves. Clinical isolates of methicillin-susceptible (MSSA, n = 20) and methicillin-resistant (mecA/mecC-MRSA, n = 20) Staphylococcus aureus were used to determine MBC by a broth microdilution method. Sodium bituminosulfonate (Ichthyol® light) was tested in double-dilution concentration steps ranging from 0.03 g/L to 256 g/L. For time-kill analysis, two reference and two clinical S. aureus strains were tested with different concentrations of sodium bituminosulfonate (1× minimal inhibitory concentration (MIC), 2× MIC, 4× MIC, 16× MIC and 256× MIC). For MSSA isolates, MBC50, MBC90 and the MBC range were 0.5 g/L, 1.0 g/L and 0.125–1.0 g/L; (MBC/MIC ratio)50, (MBC/MIC ratio)90 and the range of the MBC/MIC ratio were 4, 4 and 1–8, respectively. Among MRSA isolates, MBC50, MBC90 and the MBC range amounted to 0.5 g/L, 1.0 g/L and 0.06–1.0 g/L; (MBC/MIC ratio)50, (MBC/MIC ratio)90 and the range of the MBC/MIC ratio were 2, 4 and 1–8, respectively. Time-kill kinetics revealed a bactericidal effect after 30 min for sodium bituminosulfonate concentrations of 16× MIC and 256× MIC. The bactericidal activity against MSSA and MRSA was demonstrated for sodium bituminosulfonate. The killing was very rapid with the initial population reduced by 99.9% after only short incubation with concentrations of 16× MIC and higher.
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Harseno, Singgih, Latief Mooduto, and Eric Priyo Prasetyo. "DAYA ANTIBAKTERI EKSTRAK DAUN KEDONDONG BANGKOK (Spondias dulcis Forst.) TERHADAP BAKTERI Enterococcus faecalis Antibacterial Potency of Kedondong Bangkok Leaves Extract (Spondias dulcis Forst.) against Enterococcus faecalis Bacteria." Conservative Dentistry Journal 6, no. 2 (2016): 110. http://dx.doi.org/10.20473/cdj.v6i2.2016.110-116.
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Background. The prevalence of endodontic infection after root canal treatment caused by the Enterococcus faecalis bacteria ranged between 24-77%. It is caused by resilience and virulence from Enterococcus faecalis. An alternative solution have to be done toward irrigation of root canal wall which is effective to kill bacteria. Kedondong Bangkok (Spondias dulcis Forst.) is one of the plants or natural substance potentially as an antibacteria. The antibacterial potencies of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria could be identified by determining Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). Purpose. This study is aimed to prove antibacterial potencies by identifying Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of Kedondong Bangkok leaves extract (Spondias dulcis Forst.) against Enterococcus faecalis bacteria. Method. This study is an experimental laboratories through research design of The Post Test Only Control Group Design. Value of MIC and MBC were known by counting the growth of Enterococcus faecalis bacteria by treating the leaves extract of Kedondong Bangkok with concentration respectively 25%, 22,5%, 20%,17,5%, 15%, and 12,5% on nutrient agar media in CFU/ml. Result. In the concentration 12,5% there are 8.8% bacterial growth and in the concentration 15% there are no bacterial growth. Conclusion. Kedondong Bangkok leaf extract (Spondias dulcis Forst.) has an antibacterial potency against Enterococcus faecalis bacteria. The MIC shows in concentration of 12,5% and the MBC shows in concentration of 15%.
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MONI, Esther Del Florence NDEDI, Maximilienne Ascension NYEGUE, Jean Paul ASSAM ASSAM, et al. "Effects Of Essential Oil From Drypetes gossweileri S. Moore Stem Barks On Cell Release And Dna Synthesis Of Mycobacterium tuberculosis." Journal of Drug Delivery and Therapeutics 9, no. 2-s (2019): 319–24. http://dx.doi.org/10.22270/jddt.v9i2-s.2644.
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Background: In the recent years, the proliferation of multi-drug-resistant and extensively drug-resistant strain to tuberculosis (TB) suggest that efforts are required to find alternative treatments. The designed study aimed to show the effects of essential oils (EO) from Drypetes gossweileri stem barks on Mycobacterium tuberculosis cell membrane release and DNA synthesis. Methods: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined against two clinical isolates (IS53 and IS310) and the reference strain H37Rv ATCC 27294 using microdilution method. The effect of essential oil on cell membrane release of Mycobacterium tuberculosis was evaluated by measuring DNA, RNA and proteins release in extracellular medium using NanoDrop 1000 spectrophotometer to show the membrane integrity lose. The effect on DNA was performed by measuring genomic DNA and amplicons of MIRU 04 sequence produced when treated at MICs and MBCs concentrations to put in evidence the inhibitory effect of EO during DNA synthesis. Results: The results revealed that EO from Drypetes gossweileri stem barks exhibited strong activity with MIC ranging from 4.88 µg/mL against H37Rv and IS310 to 9.76 µg/mL against IS53. The significant release of DNA, RNA and proteins in extracellular medium were observed for treated cells at MIC and MBC concentrations compare to untreated cells. The most quantified biomolecules were proteins with concentration ranging from 370.9 104 ng/µL to 10630.0 104 ng/µL released at MIC concentration which increased from 1890.0 104 ng/µL to 12000.9 104 ng/µL at MBC. The inhibitory effect of DNA synthesis by EOs enhanced lower quantity of DNA for all treated cells at MIC and MBC compare to untreated cells. The results obtained in this study enabled the identification of two cellular targets (cell membrane and DNA) of EO from D. gossweileri stem barks on M. tuberculosis.
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Sperling, D., H. Karembe, M. Zouharova, and K. Nedbalcova. "Examination of the minimum inhibitory concentration of amoxicillin and marbofloxacin against Streptococcus suis using standardised methods." Veterinární Medicína 65, No. 9 (2020): 377–86. http://dx.doi.org/10.17221/111/2020-vetmed.
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The results of the antimicrobial susceptibility testing of clinical isolates Streptococcus suis to amoxicillin and marbofloxacin obtained by the agar dilution method and broth microdilution method with the results obtained by the commercially available E-test were compared. Comparisons between the methods based on the determination of the minimal inhibitory concentration (MIC) of the antimicrobials were assessed based on the degree and frequency of the categorical agreement (Agar dilution method as a reference system) and the percentage of the categorical agreement and error rate. A statistical evaluation was determined using the Bland-Atman method. The presented MIC values, determined for the isolates in the E-test, were slightly different from the MIC values determined by the dilution tests, mainly due to the different defined testing concentrations. For the E-test as the test system and agar-dilution method as the reference system, no error of any class was detected (very major, major and minor error) and a complete categorical agreement was obtained between the evaluated methods for amoxicillin. For amoxicillin, the regression and correlation analysis show linear relationships between the E-test and the two dilution methods with significant coefficients of determination (0.62 and 0.75). The slopes of the equality and regression lines were not significantly different. However, the E-test tends to slightly overestimate the MIC values when compared to the microdilution. The reverse is true when compared with the agar dilution. There was good agreement between the E-test and the dilution methods with a low bias (0.001 3 and −0.005 0), all the experimental data were within the computed limits of agreement. For marbofloxacin, the same trends were observed with lower coefficients of determination (0.42 and 0.73) and a less favourable agreement. The E-test constantly underestimated the MIC values when compared to the two dilution methods. No significant difference between the microdilution and agar dilution was obtained.
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Qiu, Xue Jing, Ying Zi He, and Xiao Wang. "In Vitro Antibacterial Activity of Different Polar Solvent Extracts from Oxalis." Advanced Materials Research 1033-1034 (October 2014): 240–46. http://dx.doi.org/10.4028/www.scientific.net/amr.1033-1034.240.
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The Oxalis extract was obtained by soaking with ethanol, then mixed with distilled water and successively extracted using chloroform, ethyl acetate and n-butanol. The four different polar solvent (chloroform, ethyl acetate, n-butanol, aqueous) extracts were evaporated under reduced pressure to obtain four kinds of crude extractum. Antibacterial activity on Staphylococcus aureus, Escherichia coli, Albicans Saccharomyces and Pseudomonas aeruginosa of the four fractions were studied by minimal inhibitory concentration method (MIC). The results indicated that the chloroform extract and n-butanol extract from red Oxalis have no antimicrobial activity to the above 4 bacteria; the ethyl acetate extract showed significant inhibitory activities to the above 4 bacteria, the minimal inhibitory concentration (MIC) were 2560ug/ml, 1280ug/ml, 2560ug/ml, 2560ug/ml respectively; the aqueous extract have inhibitory activity to Escherichia coli and Albicans Saccharomyces, the minimal inhibitory concentration (MIC) were 2560ug/ml, 2560ug/ml respectively, but it has no antimicrobial effect on the other two kinds of bacteria. The chloroform extract and ethyl acetate extract from yellow Oxalis have no antimicrobial activity to the above 4 bacteria; the n-butanol extract has inhibitory activity to Escherichia coli, Albicans Saccharomyces and Pseudomonas aeruginosa, the minimal inhibitory concentration (MIC) were 5120ug/ml, 5120ug/ml, 5120ug/ml respectively, but it has no antimicrobial effect on Staphylococcus aureus; the aqueous extract have inhibitory activity to Escherichia coli and Albicans Saccharomyces, the minimal inhibitory concentration (MIC) were 2560ug/ml, 5120ug/ml respectively, but it has no antimicrobial effect on the other two kinds of bacteria.
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Widelski, Jarosław, Piotr Okińczyc, Katarzyna Suśniak, et al. "Phytochemical Profile and Antimicrobial Potential of Propolis Samples from Kazakhstan." Molecules 28, no. 7 (2023): 2984. http://dx.doi.org/10.3390/molecules28072984.
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In the current paper, we present the results of Kazakh propolis investigations. Due to limited data about propolis from this country, research was focused mainly on phytochemical analysis and evaluation of propolis antimicrobial activity. uHPLC-DAD (ultra-high-pressure-liquid chromatography coupled with diode array detection, UV/VIS) and uHPLC-MS/MS (ultra-high-pressure-liquid chromatography coupled with tandem mass spectrometry) were used to phytochemical characteristics while antimicrobial activity was evaluated in the serial dilution method (MIC, minimal inhibitory concentration, and MBC/MFC, minimal bactericidal/fungicidal concentration measurements). In the study, Kazakh propolis exhibited a strong presence of markers characteristic of poplar-type propolis—flavonoid aglycones (pinocembrin, galangin, pinobanksin and pinobanskin-3-O-acetate) and hydroxycinnamic acid monoesters (mainly caffeic acid phenethyl ester and different isomers of caffeic acid prenyl ester). The second plant precursor of Kazakh propolis was aspen–poplar with 2-acetyl-1,3-di-p-coumaroyl glycerol as the main marker. Regarding antimicrobial activity, Kazakh propolis revealed stronger activity against reference Gram-positive strains (MIC from 31.3 to above 4000 mg/L) and yeasts (MIC from 62.5 to 1000 mg/L) than against reference Gram-negative strains (MIC ≥ 4000 mg/L). Moreover, Kazakh propolis showed good anti-Helicobacter pylori activity (MIC and MBC were from 31.3 to 62.5 mg/L). All propolis samples were also tested for H. pylori urease inhibitory activity (IC50, half-maximal inhibitory concentration, ranged from 440.73 to 11,177.24 µg/mL). In summary Kazakh propolis are potent antimicrobial agents and may be considered as a medicament in the future.
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ADIWIJAYA, Wilma, Riani SETIADHI, and Vinna Kurniawati SUGIAMAN. "Antibacterial potential ethanol extract of beluntas leaves (Pluchea indica L) to Streptococcus sanguinis." Journal of Syiah Kuala Dentistry Society 7, no. 2 (2023): 78–85. http://dx.doi.org/10.24815/jds.v7i2.30230.
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Recurrent aphthous stomatitis (RAS) is one of the most common oral diseases in the community, with a prevalence of 5-66%, with one of the predisposing factors being Streptococcus sanguinis. Treatment for RAS has been symptomatic and supportive, including antiseptic mouthwash such as chlorhexidine gluconate 0.2% or topical corticosteroids (triamcinolone acetonide 0.1% in Orabase). However, these drugs have some side effects. Treating herbal ingredients such as Beluntas leaves low prices and minimal side effects. The active compounds in Beluntas leaves are phenols, tannins, flavonoids, saponins, triterpenoids, essential oils, terpenoids, and many compounds known to have antibacterial activity. Methods: This study aimed to determine the minimum inhibitory level (MIC), and minimum killing rate (MBC) of 96% ethanol extract of Beluntas leaves on the growth of Streptococcus sanguinis. MIC was measured by broth microdilution technique with DMSO solvent 10% and eight concentrations of beluntas extract. Chlorhexidine gluconate 0.2% was used as a positive control for the comparison compound. Furthermore, the MBC test was carried out using the total plate count method for treatments that gave the MIC value. One Way Anova analysis with Post Hoc Tukey was used to determine the significant difference between treatments. Results: The ethanol extract of Beluntas leaves (Pluchea indica L) has a Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for the growth of Streptococcus sanguinis with a minimum inhibitory concentration of 3.95 g/mL and a minimum concentration of 7.8 g/mL. Conclusion: The ethanol extract of Beluntas leaves (Pluchea indica L) has the potential as an antibacterial against Streptococcus sanguinis. KEYWORDS: Recurrent aphthous stomatitis, Streptococcus sanguinis, ethanol extract of beluntas leaves, Minimum Inhibitory Concentration, Minimum Bactericidal Concentration
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Kikowska, Małgorzata, Jolanta Długaszewska, Marcelina Maria Kubicka, Izabela Kędziora, Jaromir Budzianowski, and Barbara Thiem. "In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species." Herba Polonica 62, no. 2 (2016): 67–77. http://dx.doi.org/10.1515/hepo-2016-0012.
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SummaryIntroduction:Due to increasing resistance against antibiotics and antifungal agents, crude plant extracts, fractions, and isolated pure compounds became a new interest as antimicrobial agents.Objectives:The antimicrobial activity of methanolic extracts and fractions ofEryngium planumL.,E. campestreL., andE. maritimumL. was evaluated against selected bacteria, yeast and mould, and compared in testedEryngiumspecies and in their organs.Methods:The antimicrobial activity was studied with use of broth microdilution method. The antibacterial (Staphylococcus aureus,Pseudomonas aeruginosa) and antifungal (Candida albicans,Aspergillus niger) activity of selected extracts and fractions compared with the reference substance was expressed by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal/Fungicidal Concentration (MBC/MFC). The extract and fraction compounds were identified on the basis of TLC examination.Results:The saponin-phenolic acid fractions ofE. maritimumandE. planumand a saponin fraction ofE. planumshowed the highest activity againstS. aureus(MIC = 1–2.5 mg·ml−1). The growth ofC. albicanswas inhibited by methanolic extract ofE. planumcell suspension culture (MIC = 7.8 mg·ml−1).Conclusion:The antimicrobial activity depends on theEryngiumspecies, tested biomass, and microorganism.
Dissertations / Theses on the topic "Minimal Inhibitory Concentration (MIC) method"
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Cerqueira, Valdeane Dias. "Composi??o qu?mica do ?leo essencial de Lippia origanoides Kunth e atividade antimicrobiana frente a diferentes sorotipos de Haemophilus parasuis." Universidade Estadual de Feira de Santana, 2014. http://localhost:8080/tede/handle/tede/123.
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Previous issue date: 2014-03-28<br>Pig farming has become increasingly important in recent years in Brazil, because of this, studies for the treatment of diseases that cause the loss of mass of meat animals has increased significantly, such as the Glasser's disease caused by Haemophilus parasuis. Some initial studies have shown human resistance to antibiotics due to the consumption of meat produced with high levels of these substances, and alternatively treatments have been developed from natural products. Lippia origanoides Kunth is presented as a natural source of antimicrobial substances due to the composition of the essential oil obtained, mainly, from the leaves of this plant. In this study the antimicrobial activity of the essential oil from Lippia origanoides Kunth, by determining the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Haemophilus parasuis serotypes 1,2,4,5,9,10,12,13,14 and one untypable was studied. Essential oils were obtained by hydrodistillation of the dried leaves and the chemical composition analysis revealed the presence of carvacrol as the predominant component, which characterizes the chemotype B. The results of the antimicrobial activity demonstrated the inhibitory effect of essential oil samples for all tested bacteria. The best result was 0.005% against the sample MV12315 (serotype 10) while the least satisfactory was 0.078% against the sample MV12196 (serotype 12). Results demonstrate the bactericidal action of the oil against the different serotypes of Haemophilus parasuis.<br>A suinocultura vem se sobressaindo nos ?ltimos anos no Brasil, por isso aumentam os estudos para tratamento das doen?as que causam perdas de carca?a dos animais, como a doen?a de Gl?sser, provocada pelo Haemophilus parasuis. Alguns trabalhos incipientes demonstram a resist?ncia humana a antibi?ticos devido ao consumo de carnes produzidas com altos ?ndices destas subst?ncias, e tratamento alternativos com produtos naturais vem sendo desenvolvidos. Lippia origanoides Kunth se apresenta como uma fonte natural de subst?ncias antimicrobianas devido ? composi??o do seu ?leo essencial obtido principalmente das folhas desta planta. Este trabalho avaliou a atividade antimicrobiana do ?leo essencial de Lippia origanoides atrav?s da determina??o da Concentra??o Inibit?ria M?nima (CIM) e Concentra??o Bactericida M?nima (CBM) frente a amostras de campo do Haemophilus parasuis com sorotipos 1,2,4,5,9,10,12,13,14 e um n?o sorotip?vel. Os ?leos essenciais foram obtidos por hidrodestila??o das folhas secas ap?s tr?s horas, e na an?lise da composi??o qu?mica, o carvacrol foi identificado como componente predominante, caracterizando-o como quimiotipo B. Os resultados de atividade antimicrobiana demonstram o efeito inibit?rio do ?leo essencial para todas as amostras de bact?rias testadas. O melhor resultado encontrado foi de 0,005% frente a amostra MV12315 (sorotipo 10) enquanto o menos satisfat?rio foi de 0,078% contra a amostra MV12196 (sorotipo 12). Os resultados obtidos demonstram a a??o bactericida do ?leo para os diferentes sorotipos do Haemophilus parasuis.
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Zavřelová, Alena. "Vyhodnocení aktivity potenciálně antimykotických látek pomocí mikrodiluční bujónové metody III." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-334695.
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Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of thesis: Evalution of activity of potentional antimycotic substances through the use of microdilution broth method III Autor of thesis: Alena Zavřelová Supervisor: Mgr. Klára Konečná, Ph.D. Background: The aim of this thesis, due to the growing incidence of fungal diseases and the growing incidence of resistance, was to determine the activity of potentially antimycotic active substances. We tested newly synthesized compoused, structurally based on the substance of pyrazinamide which is significant antituberculotic. Methods: Testing of substances was carried out using a broth microdilution method on eight strains of pathogenic fungi consisting of five yeast strains: Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, Trichosporon asahii and three strains of filamentous fungi: Aspergillus fumigatus, Absidia corymbifera, Trichophyton mentagrophytes. Results: From the range of tested concentrations and from the total number of the thirty tested substances, the most noticeable antifungal aktivity was revealed in the substance of 5-methyl-6-(2-phenylhydrazinyl)pyrazine-2,3- dicarbonitrile. All strains, except Trichophyton mentagrophytes strain, showed a relatively...
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Kopečná, Klára. "Vyhodnocení aktivity potenciálně antibiotických látek pomocí mikrodiluční bujónové metody II." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343393.
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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of biological and medical sciences Study program: Pharmacy Autor: Klára Kopečná Supervisor: Mgr. Klára Konečná, Ph.D. Title of diploma thesis: Evaluation of activity of potencional antibiotic substances through the use of microdilution broth method II Background: To evaluate the antibacterial activity of the compounds synthesized by the Department of Pharmaceutical Chemistry and Drug control, Faculty of Pharmacy in Hradec Králové, Charles University in Prague under the leadership of prof. PharmDr. Martin Doležal, Ph.D. Methods: Using broth microdilution method was tested antibacterial activity at eight bacterial strains, namely Staphylococcus aureus, Staphylococcus aureus methicilin resistant, Staphylococcus epidermidis, Enterococcus sp., Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae ESBL positive and Pseudomonas aeruginosa. Results: Test substances were divided into 5 groups according to similarities in the chemical structure. The best antibacterial activity was observed in the group of derivatives of N-benzyl- 6-(alkylamino)pyrazine-2-carboxamide. Conclusion: Antibacterial activity was detected in 10 of 24 tested compounds. From active substances, all but one danced only Gram-positive bacteria. Among...
Book chapters on the topic "Minimal Inhibitory Concentration (MIC) method"
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Nahler, Gerhard. "minimal inhibitory concentration (MIC)." In Dictionary of Pharmaceutical Medicine. Springer Vienna, 2009. http://dx.doi.org/10.1007/978-3-211-89836-9_861.
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Pacheco, Patricia, Dévora Carrión, Sandy Gavilanes, and Mónica Espadero. "In vitro Antibacterial Activity of Ethanolic and Acetonic Extracts of Curcuma longa Against Propionibacterium acnes (Cutibacterium acnes)." In Lecture Notes in Networks and Systems. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-87065-1_27.
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Abstract Throughout history, plants have been indispensable in the development of society due to their high content of bioactive substances, which are used in different industries such as food, pharmaceutical, and cosmetics. Curcuma longa is a perennial species that belongs to the Zingiberaceae family, which is used for therapeutic purposes due to its anti-oxidant, anti-inflammatory and antibacterial properties, among others. This research evaluates the antibacterial activity of ethanolic and acetonic extracts of Curcuma longa against Propionibacterium acnes, recently renamed Cutibacterium acnes, that has long been implicated in the pathogenesis of acne. Secondary metabolites were identified by phytochemical screening tests. Curcuminoids determination was carried out by using TLC (thin-layer chromatography) and the phenolic compounds were quantified by the Folin-Ciocalteu assay. The antibacterial activity was evaluated using the Kirby-Bauer method with different treatments (50,75 and 100%) and broth microdilution method was used to determine the minimum inhibitory concentration (MIC). The extracts showed the presence of phenolic compounds, flavonoids, terpenoids, and tannins, confirming the presence of curcuminoids. In the quantification of phenolic compounds, no significant differences in their composition were observed. The treatment 100% of acetonic extract of Curcuma longa exhibited an inhibition percentage of 48.79% compared to the ethanolic ex tract 75% treatment, which reached 31.67%. Additionally, the acetonic extract showed a minimum inhibitory concentration of 15.62 µg/mL, while for the ethanolic extract, it was 31.25 µg/mL. These findings highlight the potential antibacterial activity of the acetonic extract against Cutibacterium acnes, which could make it a promising natural option for acne control.
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Srivastava, Anmol, Vivek Kumar, and Vishnu Agarwal. "Antimicrobial Activity of Some Essential Oils Against Pseudomonas aeruginosa." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_4.
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AbstractThe emergence of multidrug resistance in bacteria due to overuse of antibiotics is becoming an important health concern in recent years, which requires development of novel alternatives to fight against these bacteria. Essential oils (EOs) are secondary metabolites that have different components and chemical compositions which may provide promising solution to the problem of rising number of drug resistant bacteria, as they can effectively kill bacteria. Here, in this study our aim is to determine the efficacy of lemongrass, rosemary, clary sage, geranium and tea tree essential oil against Pseudomonas aeruginosa, using agar well diffusion method. The minimum inhibitory concentration (MIC) of these EOs were also determined. The chemical composition of these essential oils were known by gas chromatography-mass spectrometry (GC-MS) analysis. It was revealed in this study that most of the essential oils show antimicrobial property against the test bacterium. The MIC of lemongrass is 0.25% (v/v), rosemary is 1% (v/v), clary sage is 2% (v/v), geranium is 0.5% (v/v) and for tea tree oil is 1% (v/v). We can infer from this data that lemongrass, rosemary, clary sage, geranium and tree oil can be utilized to treat infections caused by Pseudomonas aeruginosa, which is a gram-negative bacterium.
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Bou, German. "Minimum Inhibitory Concentration (MIC) Analysis and Susceptibility Testing of MRSA." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-468-1_3.
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Molina-Gallardo, Axel R., Yesica R. Cruz-Martínez, Julieta Orozco-Martínez, Israel Valencia Quiroz, and C. Tzasna Hernández-Delgado. "The Roles of Farnesol and Farnesene in Curtailing Antibiotic Resistance." In Biotechnology and Drug Development for Targeting Human Diseases. BENTHAM SCIENCE PUBLISHERS, 2024. http://dx.doi.org/10.2174/9789815223163124090005.
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In the extensive domain of “biotechnology and drug development for targeting human diseases”, essential oils have long been revered for their therapeutic potential. Among these, farnesol and farnesene stand out due to their pharmacological attributes. As the challenge of antibiotic resistance intensifies, the scientific community is increasingly exploring the potential of these traditional remedies. Using the KirbyBauer agar diffusion method, a qualitative assessment was conducted on two grampositive and two gram-negative bacterial strains. The broth microdilution technique further determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and the sensitizing impacts of these compounds. Both farnesol and farnesene exhibited antibacterial efficacy against all evaluated strains. Their synergistic potential was highlighted when combined with clavulanic acid, cefuroxime, and cefepime. Among these combinations, farnesene paired with cefepime showed pronounced efficacy against Escherichia coli 82 MR, with an MIC of 0.47 μg/mL. In contrast, in the investigation of Staphylococcus aureus 23MR, it was observed that this particular strain exhibited an increased sensitivity when exposed to combinations containing farnesol. Notably, the Minimum Inhibitory Concentration (MIC) was determined to be 0.03 µg/mL in the presence of both antibiotic agents. To gain deeper molecular insights, docking experiments were performed with the βlactamases of E. coli and S. aureus, focusing on the most effective combinations. All tested compounds—cefuroxime, cefepime, farnesene, and farnesol—acted as noncompetitive inhibitors, suggesting their potential mechanisms of action.
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Arcani, Bianca Cristina Melo, and Deise Helena Baggio Ribeiro. "Evaluation of the Antimicrobial Potential of Seeds Extracts from Fruits of the Myrtaceae Family." In Métodos Microbiológicos Tradicionais e Avançados para a Segurança de Alimentos. Editora Científica Digital, 2025. https://doi.org/10.37885/250519354.
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Objective: Fruit seeds, often discarded as waste by the food industry, are underutilized source of bioactive compounds. While the antimicrobial activity of Myrtaceae species leaves is well known, few studies have investigated its seeds for this purpose. This study evaluated the antimicrobial potential of hydroalcoholic extracts from seeds of cambuci (Campomanesia phaea), grumixama (Eugenia brasiliensis), jabuticaba (Plinia cauliflora), and pitanga (Eugenia uniflora). The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined to Bacillus cereus, Candida albicans, Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, and Pseudomonas aeruginosa. Method: The ethanolic extracts were concentrated, the antimicrobial potential was assessed using the agar diffusion method, the MICs was determined by microdilution and MBC by drop plate technique. Results: All extracts showed inhibitory potencial. The lowest MICs were determined to grumixama extract (E. coli; 0.04 g/mL), followed by jabuticaba and pitanga (E. coli, L.monocytogenes, S. Typhimurium, and S. aureus; 0.06 g/mL), and cambuci (L. monocytogenes and S. Typhimurium; 0.07 g/mL). Although all extracts showed bactericidal activity at concentrations ranging from 0.06 to 0.95 g/mL, the lowest MBC was observed on pitanga’s extract to E. coli, S. Typhimurium, and S. aureus. Conclusion: The results highlight the potential of seeds as an alternative source of antimicrobial compounds, contributing to the valorization of industrial by-products and reducing the discard of materials with potential technological applications.
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Fernandes, C. C., M. B. Santiago, C. H. G. Martins, J. B. A. Silva, A. E. M. Crotti, and M. L. D. Miranda. "ESSENTIAL OIL FROM EUGENIA PYRIFORMIS CAMBESS AERIAL PARTS AND ITS MAJOR CONSTITUENT LIMONENE AS AN AGENT WITH ANTI-ACETYLCHOLINESTERASE AND ANTICARIOGENIC ACTIVITIES." In Open Science Research XII. Editora Científica Digital, 2023. http://dx.doi.org/10.37885/230613489.
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This study aimed at determining, for the first time, anticariogenic and anti-acetylcholinesterase activities of essential oil from Eugenia pyriformis aerial parts (EP-EO) and its major constituent limonene against cariogenic bacteria and the enzyme acetylcholinesterase (AChE). EP-EO and limonene were subject to the broth microdilution method on 96-well microplates and determination of minimum inhibitory concentration (MIC) and minimum inhibitory concentration of biofilm (MICB50) (µg/mL). AChE inhibition was qualitatively evaluated by Thin Layer Chromatography (TLC). EP-EO and limonene not only showed strong anticariogenic activity, since their MIC and MICB50 values ranged between 20 and 400 µg/mL, but also inhibited cholinesterase when subject to TLC. Both GC-MS and GC-FID analyses identified and quantified the following major constituents: limonene (14.8%), nerolidol (11.0%) and α-cadinol (10.3%). This study showed that EP-EO and limonene were active in in vitro assays and, thus, may be important targets of further in vivo studies.
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Gălăţanu, Mona Luciana, Mariana Panţuroiu, Mariana Popescu, and Carmen Marinela Mihăilescu. "Plant Extracts With Antibiotic Effect." In Handbook of Research on Advanced Phytochemicals and Plant-Based Drug Discovery. IGI Global, 2022. http://dx.doi.org/10.4018/978-1-6684-5129-8.ch004.
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This chapter tries to describe the most important plant extracts and their bioactive compounds which determine the antibiotic activity. Pharmacological assays performed for each plant extract are presented, including the minimum inhibitory concentration (MIC) as the most used experimental method to determine antimicrobial activity. Also, the effective associations between classic antibiotics and plant extracts with antibacterial are presented. The mechanisms of action are deeply explained to the extent that they are known and discovered by in vitro and in vivo studies. Plant-derived compounds have different mechanism of action as antibiotics. They can have other target sites than traditional antimicrobials and subsequently having different mechanisms of action against microbes. Ultimately, this chapter tries to be an invitation to use plant extract as an alternative to chemical, synthetic antibiotics, or used complementary, synergistic for better therapeutically results.
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de Sousa, Danyelle Gomes, Yasmin Macedo de Sousa, Kelry de Souza Fechine Andrade, et al. "GC-MS ANALYSIS AND IN VITRO ANTIMICROBIAL ACTIVITY OF Ocotea catharinesis MEZ essential oil (LAURACEAE)." In Knowledge Integration: A Multidisciplinary Approach to Science. Seven Editora, 2025. https://doi.org/10.56238/sevened2025.018-046.
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The use of plants to treat and prevent diseases is an ancestral practice of humanity. In recent years, the resistance of microorganisms to antibiotics has generated a growing interest in the research of therapeutic alternatives, including the chemical compounds present in essential oils. The aim of the present study was to evaluate the antibacterial and antifungal effect of Ocotea catharinesis essential oil (EOOc) and to identify its main compounds. The extraction of the essential oil was done by hydrodistillation for 4.5 hours, using 50g of dried leaves. To identify the chemical components, gas chromatography with mass spectrometry was used. For the microbiological tests, the strains tested included Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. The Minimum Inhibitory Concentration (MIC) was determined by the microdilution method. The essential oil was tested as a modulator of antibiotic resistance, using gentamicin, penicillin and norfloxacin as references for antibacterials and fluconazole for fungi, with or without the presence of the oil. Analysis of the oil revealed two main compounds: alpha pinene (13.9%) and limonene (13.4%). Alpha pinene, a terpenoid, can help reduce oxidative stress by acting as an antioxidant. The cEOOc showed resistance against E. coli and P. aeruginosa bacteria, with values equal to or greater than 1024 μg/mL, showing that gram-negative bacteria are more resistant. With the antibiotic penicillin, the EOOc showed similar results, also because of receptor proteins. Tests with Norfloxacin and Gentamicin showed values of 512 μg/mL, with no synergistic potential with antibiotics. It was observed that against the strains of Candida tropicalis, the EOOc presented a modulating effect with a lower concentration than the drug tested. The results indicate that the essential oil of Ocotea catharinesis Mez may have an antifungal effect against Candida tropicalis strains.
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Soares Gettens, Cristina, Thauana Heberle, Nadia Carbonera, Eliezer Avila Gandra, Aline Machado Pereira, and Marcia Arocha Gularte. "ANTIMICROBIAL POTENTIAL AND CHEMICAL AND BIOACTIVE COMPOUNDS IN AGROINDUSTRIAL BY PRODUCTS FROM PEACH." In Ciência e Tecnologia de Alimentos: o avanço da ciência no Brasil - Volume 3. Editora Científica Digital, 2023. http://dx.doi.org/10.37885/230312287.
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Objective: The objective was to determine the antimicrobial potential and evaluate the chemical and bioactive compounds in the by-products of peach syrup production, which were “substandard peaches” and the peach kernel almond and its extracts. Methods: The analyses included total soluble solids, pH, total titratable acidity, centesimal composition, vitamin C, carotenoids, total antioxidant activity, total phenols, antifungal potential, antibacterial activity, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The most concentrated extracts from the peach and peach kernel almond presented higher antioxidant activity in IC50, with levels of 2.66 and 7.88 μg. mL-¹ respectively. The total phenol content was 253.4 mg GAE.100g-¹ for the peach and 29.3 mg GAE.100g-¹ for the almond. The extracts tested showed no antifungal potential; however, the extracts of peach and almond presented antibacterial potential against S. Typhimurim and S. aureus bacteria. The MIC for kernel a l m o n d and peach extracts against S. aureus was 0.75 mg.mL-1 for both, but these did not present CBM. The extracts obtained from the peach by-products did not present antifungal activity, but the peach almond showed an inhibitory effect on S. aureus. Conclusion: The peach and peach kernel almond presented significant antioxidant activity and phenolic compound contents, owning high levels of bioactive compounds.
Conference papers on the topic "Minimal Inhibitory Concentration (MIC) method"
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Jones, C. R., M. J. Hails, and B. L. Downward. "New Convenient Analytical Method for Biodegradable Squeeze Scale Inhibitors." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07062.
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Abstract Traditionally, phosphonate based scale inhibitors have dominated the global squeeze treatment market and for very good reasons: they combine high performance with ease of measurement. However, increasingly stringent environmental legislation has resulted in the development of new, enhanced polymeric scale inhibitors that contain low levels of phosphorus, such as phosphonate end-capped polymers. These polymers are highly effective with particularly low MIC (minimum inhibition concentration) values, but this creates analytical problems as they can’t currently be easily measured at these low levels. Therefore, to realise the full performance and environmental benefits of such polymers it is necessary to be able to readily and easily measure them at low concentrations in a variety of brine chemistries. A novel separation and pre-concentration procedure has been developed that can be combined with industry standard analytical methods (e.g. Hyamine) to detect such scale inhibitors. It is simple to use, flexible, requires no specialist knowledge or equipment and can measure sub-ppm levels of inhibitor reliably and accurately in even the most concentrated brines.
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Shen, Dong, Jeffrey Russek, Haiping Lu, Larry Hines, and Michael Jordon Oney. "How to Acquire a Reliable Scale Inhibitor MEC Value Based on Laboratory Testing Results – a Field Case Study." In CONFERENCE 2022. AMPP, 2022. https://doi.org/10.5006/c2022-17623.
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Abstract Laboratory scale inhibitor performance tests have been widely accepted to screen products and determine the minimum effective concentrations (MEC) of selected products for field treatment. The dynamic tube-blocking test is the most common lab testing method used to obtain an inhibitor MEC number because the testing temperature, oxygen level, pH, and pressure can be well controlled to duplicate the field scaling conditions. A new benchtop testing method, the Kinetic Turbidity Test (KTT), has drawn increased attention for scale inhibitor evaluation and MEC determination under some scaling circumstances due to its ability to monitor the formation and growth of mineral scales continuously throughout the test duration. In west Texas, a well being evaluated had failed three times within 1.5 years because of the formation of scale on the ESP pump, even though it had an aggressive squeeze treatment with the inhibitor residuals of 20 ppm and above. Both lab dynamic tube-blocking tests and KTT results show that 5 ppm of the selected scale inhibitor would be sufficient to prevent the formation of scales for a general produced water sample. However, under the worst scaling scenario according to historical produced water data, KTT results indicate that more than 30 ppm of scale inhibitor is required to achieve an effective scale treatment, whereas the dynamic tube-blocking test MEC is still well below 20 ppm. This paper is intended to give some insight on the field treatment failure analysis by comparing the difference between the MEC obtained from different testing methods at various scaling conditions. The other possible reasons that may cause treatment failure, such as uneven scale inhibitor distribution at different producing zones and inhibitor residual samples contamination, are also included in this study.
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Aldeia, W., J. C. Del Bigio, V. S. Lourenço, A. L. Martins, B. B. Castro, and H. E. Schluter. "Possible Increase of Production Through Magnetic Field and Inhibitor Association - Evaluation of Reduction Minimal Inhibitory Concentration - MIC in a Brazilian Pre-Salt Well." In Offshore Technology Conference Brasil. OTC, 2023. http://dx.doi.org/10.4043/32696-ms.
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Abstract This article presents the results obtained in the evaluation of the joint action of the chemical inhibition (inhibitor) and the magnetic field, aiming to evaluate the possibility of reducing the minimal inhibitory concentration (MIC), seeking an increase in production in oil extraction wells, increasing the reliability of the guarantee of flow in deep water wells. The magnetic field has the potential to retard the scaling of inorganic salts in oil production/water treatment systems. An evaluation of the combination of magnetic field and inhibitor to retard carbonate scaling was carried out, based on the tube blocking test method (TBT). The evaluation was carried out in two brine solutions, with reduced concentrations from a brine solution representative of the Brazilian pre-salt. A reactor with an internal diameter of 0.045 cm and a total flow rate of 20 cm3/min was used. (Re ≈ 900), reactor inlet pressure of 10 kgf/cm2 and outlet pressure of 1 kgf/cm2. The temperature was 60 °C and the inhibitor used was hydroxymethyl amino-di(methylene phosphonic acid) type in different concentrations. The magnetic field used was 1.0 Tesla. The results indicate that the association of inhibitor and magnetic field under certain conditions can lead to significant delays in calcium carbonate fouling, proving to be an interesting strategy for mitigating calcium carbonate fouling.
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A.GHAFIL, Jenan, Nihad Taha Mohammed JADDOA, and Marwa shakib ALRAWI. "ACALYPHA AUSTRALIS PLANT PROMISING TREATMENT AGAINST BACTERIA." In VI.International Scientific Congress of Pure,Applied and Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress6-31.
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The research aimedtoevaluate theantibacterial effect of ethanol extract of Acalypha australisagainst eightbacterialstrains (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Actinomyces, Proteus mirabilis and Streptococcus pneumonia) with concentrations ranged from 3.90 to 2000 µg/ml. The sensitivity of bacterial isolates to various antibiotics was tested by VITIK2 Densi-Check equipment. The xtricate was made by a soaked powder of Acalypha australis with 80%ethanol in the unit of soxhlet extractions and after that was aseptically sifted. The antibacterial effects of theextricate were surveyed utilizing the agar dissemination strategy and the broth microdilution-method, which was utilized to gauge the extract's minimal inhibitory concentration (MIC). Results appearedthat the ethanol-extricate has antibacterial action in a concentration-dependent way, with the normal distance across zone of hindrance watched against bacterial segragates extending from 15±0.5 mm to 25±0.5 mmThe xtract had the greatest effectagainst E. coli, followed by P. aeruginosa, and the least effect against P. mirabilis. The extract's minimal inhibitory concentration varies from one species to another, ranging from 31.25 to 250 µg/ml.
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Damayanti, Dini Sri, Putra Agung Dewata, and Nuris Bashirotul Hidayah. "Antibacterial Effect of Aloe vera Ethanol Extract Against the Growth of Bacteria Staphylococcus aureus and Escherichia coli through ZOI Test, MIC and MBC Measurement." In 8th International Students Science Congress. ULUSLARARASI ÖĞRENCİ DERNEKLERİ FEDERASYONU (UDEF), 2024. https://doi.org/10.52460/issc.2024.008.
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Staphylococcus aureus is a gram-positive bacterium while Escherichia coli is a gram-negative bacterium that often causes infections and be used as a bacteriological model for many antibacterial screenings. Aloe vera is known to have active compounds that act as antibacterial, however, the effect of Aloe vera ethanol extract on the inhibition of growth and death of Staphylococcus aureus and Escherichia coli bacteria is not clear yet. Aloe vera was extracted using maceration in ethanol. Phytochemical tests were carried out to de-termine the active compounds in the extract [3]. To measure antibacterial efficacy, a disc diffusion method using concentrations of 3,125%, 6,25%, 12,5%, 25%, 50%, 100%, control (+) and control (-) was done using S. aureus and E. coli. Data analysis using One Way Analysis of Variance (ANOVA) test and then continued Tukey's Post Hock test with a significance of p< 0.05 [4] Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was then determined with the same concentrations. The study's Aloe vera ethanol extract contained flavonoids and alkaloids. The zone of inhibitions diameter on both bacteria at the concentration of 3.125%, 6.25%, 12.5%, 25% and the negative con-trol were 0 mm. At 50% concentration, the diameter was 7.67±0.58 mm, while at 100% concentra-tion was 20.67±1.53 mm. The diameter of tetracycline and amoxicillin were 34 mm and 36 mm, respectively. MIC and MBC of Aloe vera ethanol extract against Staphylococcus aureus at a concen-tration of 12.5% while against Escherichia coli at a concentration of 25%. Aloe vera ethanol extract has antibacterial effects and is able to inhibit and kill Staphylococcus aureus and Escherichia coli bacteria with higher inhibitory power against Staphylococcus aureus than against Escherichia coli.
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Todorović, Jovana D., Aleksandra D. Vesić, Nevena N. Petrović, and Marijana M. Kosanić. "Antimicrobial potential of mushrooms Macrolepiota procera and Chlorophyllum rhacodes." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.304t.
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The aim of this study is to investigate the in vitro antimicrobial properties of the acetone extracts of the mushrooms Macrolepiota procera and Chlorophyllum rhacodes. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and assess the antimicrobial effects against three bacterial and ten fungal species. Overall, the tested mushroom extracts had relatively strong antimicrobial activity against the tested microorganisms. The minimum inhibitory concentration for both extracts related to the tested bacteria and fungi was 0.625 – 20 mg/mL. Extract of C. rhacodes exhibited more powerful antimicrobial properties, with ranged MIC values from 0.625 mg/mL to 10 mg/mL. The Acetone extract of C. rhacodes has shown the most antibacterial activity against S. aureus, while the extract of M. procera has not shown activity against S. aureus as against G. candidum. In comparison to the standard antibiotics as positive controls, the antimicrobial activity of studied extracts was less expressed. The results suggest that mushroom species may be used for pharmaceutical purposes in treating various diseases.
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Panić, Jovana J., Snežana M. Papović, Teona Teodora V. Borović, et al. "The hydration and antimicrobial properties of selected imidazole-based ionic liquids with a homologous series of chloride oxyanions." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.124p.
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The aim of this work was to get a detailed insight into the ion’s interactions along with the structure-making/structure-breaking tendency that has been retrieved through the perusal of calculated parameters from volumetric measurements for aqueous solutions of three newly synthesized ionic liquids: 1-butyl-3-methylimidazolium chlorite, 1-butyl-3-methylimidazolium chlorate and 1-butyl-3-methylimidazolium perchlorate. Further, the antimicrobial activity of synthesized and commercial (1-butyl-3-methylimidazolium chloride) ionic liquids on certain strains of bacteria and fungi was obtained. Antimicrobial tests were performed using the in vitro microdilution method against isolated strains of Escherichia coli, Staphylococcus aureus, Bacillus cereus bacteria, and the fungus Candida guilliermondii. This method is a rapid, quantitative method for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using small amounts of samples (µl) and test compound. Based on the obtained results, the influence of the homologous series of chloride oxyanions on hydration and antimicrobial properties of imidazole-based ionic liquids will be discussed.
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Mudhar Bintang, Muhammad Ali Khumaini, and Teerapol Srichana. "Antibacterial Activity and <i>In Vitro</i> Cytotoxicity of Colistin in Sodium Deoxycholate Sulfate Formulation." In 5th International Conference and Exhibition on Pharmaceutical Sciences and Technology 2022. Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-19rsw3.
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Colistin has its problem with nephrotoxicity despite its capability for combatting multi-drug resistant gram-negative bacteria. Sodium deoxycholate sulfate (SDCS) has been shown to increase the safety profile of nephrotoxic drugs. This study aimed to explore the antimicrobial activity of colistin-SDCS versus free colistin against P. aeruginosa and investigate their cytotoxicity on kidney cells. The colistin micelles were formulated with SDCS followed by lyophilization and their properties were analyzed. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of colistin were determined using the broth microdilution method. The static time-kill kinetics were also employed to monitor the bactericidal activity of formulation over time. The cytotoxicity of formulations was analyzed using MTT colorimetric assay against kidney cells. The colistin-SDCS dry-powder was stable after reconstitution and resulted in 240 to 297 nm in particle size with the zeta potential of -22.8 to -23.4 mV. The colistin-SDCS formulations showed similar antibacterial activity against P. aeruginosa to pure colistin. MIC and MBC were 7.81 and 15.63 µg/mL, respectively. The static-time kill results displayed slightly better bactericidal activity at 24 h. The viability of kidney cells exposure to colistin-SDCS micelle was higher than that of pure colistin.
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Katanić Stanković, Jelena S., Nikola Srećković, and Vladimir Mihailović. "PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.359ks.
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In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.
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de Souza, Heide Mendonça Moreira, Karen Cristina Comin Maldonado, Carla Alciati, and Cristina Lúcia Feijó Ortolani. "Analysis of the antibacterial efficacy of Plantago major L. extract against Streptococcus mutansby microdilution in broth." In VI Seven International Multidisciplinary Congress. Seven Congress, 2024. http://dx.doi.org/10.56238/sevenvimulti2024-050.
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The present study investigated the efficacy of Plantago majorL. (Plantaginaceae) extract against Streptococcus mutans, a bacterium associated with the development of biofilms and dental caries. The main objective was to determine the efficacy of this extract by means of the broth microdilution method, seeking to establish the Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of the extract. For this, bacterial suspensions were prepared and incubated with different concentrations of the extract in BHI medium. After 48 hours at 36°C, the turbidity of the samples was evaluated, followed by subculture to check for bacterial growth. The extract of Plantago majorand 1% chlorhexidine were tested, and the MIC of the extract against S. mutansat a concentration of 1.5 x 107 CFU/mL was determined at 25 mg/mL. The results indicate that the extract has significant antimicrobial activity against S. mutans, suggesting that it is a promising natural alternative for the prevention of dental caries. The comparison with chlorhexidine suggests that the extract may be a viable option, especially considering the growing demand for natural antimicrobial agents. In conclusion, this study confirms the efficacy of Plantago major extract against Streptococcus mutans, highlighting its potential as a natural antimicrobial agent. Further research is needed to further explore the mechanisms of action and efficacy of this extract in in vivo models, aiming at its development as a treatment for caries prevention
Reports on the topic "Minimal Inhibitory Concentration (MIC) method"
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ตัณฑวนิช, ทิพวรรณ, มาลินี จงเจริญใจ, วีณา เคยพุดซา, Tippawan Tantawanich, Malinee Jongjareanjai та Weena Koeypudsa. ประสิทธิภาพของสารสกัดหยาบจากสมุนไพรไทย ในการยับยั้งเชื้อแบคทีเรีย Vibrio spp. ที่ก่อให้เกิดโรคในหอยหวาน (Babylonia areolata Link 1807) : รายงานวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2013. https://doi.org/10.58837/chula.res.2013.87.
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ศึกษาประสิทธิภาพของสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิด ได้แก่ ฟ้าทะลายโจร ข่า และใบฝรั่งโดยแบ่งการทดลองออกเป็น 4 ขั้นตอน ขั้นตอนที่ 1 หาค่าความเข้มข้นต่ำสุดในการยับยั้งการเจริญเติบโตของเชื้อแบคทีเรีย Vibrio alginolyticus ที่ทำให้เกิดโรคปากบวมแดงในหอยหวาน (Minimal Inhibitory Concentration: MIC) และค่าความเข้มข้นต่ำสุดในการกำจัดเชื้อแบคทีเรีย (Minimal Bactericidal Concentration: MBC) พบว่าสารสกัดหยาบใบฝรั่งที่สกัดด้วยน้ำกลั่น โดยวิธีการต้มมีประสิทธิภาพในการยับยั้งและกำจัดแบคทีเรีย V.alginolyticus ได้สูงสุด (MIC = 2.73 mg/L และ MBC = 21.88 mg/L) รองลงมาคือ สารสกัดหยาบข่าที่สกัดด้วยเอธานอล 100% และสารสกัดหยาบข่าที่สกัดด้วยน้ำกลั่น (MIC = 2.73 – 10.94 mg/L และ MBC = 21.88 – 43.75 mg/L) ส่วนสารสกัดหยาบฟ้าทะลายโจรมีประสิทธิภาพในการกำจัดแบคทีเรียได้ต่ำสุด (MIC = 5.47 mg/L และ MBC = 87.50 mg/L) ขั้นตอนที่ 2 หาค่าความเข้มข้นของเชื้อแบคทีเรีย V. alginolyticus ที่ทำให้หอยหวานตายลง 50 เปอร์เซ็นต์ ภายในเวลา 24 ชั่วโมง (Median lethal Dose at 24 hr: LD₅₀ at 24 hr) ด้วยวิธีการฉีดเชื้อเข้ากล้ามเนื้อเท้าของหอยหวาน พบว่ามีค่า LD₅₀ at 24 hr เท่ากับ 1.426 x 10⁸ cfu/ml ขั้นตอนที่ 3 ทดสอบหาความเป็นพิษของสารสกัดหยาบจากสมุนไพรไทยทั้ง 3 ชนิดที่ทำให้หอยหวานตายครึ่งหนึ่ง (50 เปอร์เซ็นต์) ภายในเวลา 96 ชั่วโมง โดยวิธีการแช่ พบว่าหอยหวานที่เลี้ยงในสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิดจะมีอาการกระวนกระวายและดิ้นไปดิ้นมาทันทีหลังจากแช่ในสารสกัด โดยพบว่าสารสกัดหยาบฟ้าทะลายโจรมีความเป็นพิษระดับสูงที่สุด LD₅₀ at 96 hr = 385.25 มิลลิกรัม/ลิตร) รองลงมาคือ สารสกัดหยาบข่า LD₅₀ 96 hr = 809.39 มิลลิกรัม/ลิตร) และสารสกัดหยาบใบฝรั่งมีความเป็นพิษระดับต่ำที่สุด (LD₅₀ 96 hr = 3,017.11 มิลลิกรัม/ลิตร) และขั้นตอนที่ 4 ศึกษาประสิทธิภาพของสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิดในการรักษาโรควิบริโอซีสในหอยหวานด้วยวิธีการแช่ต่อเนื่องเป็นเวลา 14 วัน พบว่าสารสกัดหยาบข่ามีประสิทธิภาพในการป้องกันและรักษาโรคติดเชื้อในหอยหวานได้ดีที่สุด โดยระดับความเข้มข้นของสารสกัดหยาบฟ้าทะลายโจร ข่าและใบฝรั่งที่มีประสิทธิภาพในการป้องกันรักษาโรคสูงสุดคือ ระดับความเข้มข้น 18.75 และ 37.50 มิลลิกรัม/ลิตร ตามลำดับ แต่อัตราการรอดชีวิตของหอยหวานที่แช่ในสารสกัดหยาบสมุนไพรทั้ง 3 ชนิด ไม่มีความแตกต่างกับชุดควบคุมบวกที่ได้รับเชื้อแบคทีเรียแต่ไม่ได้แช่สารสกัดสมุนไพร จากการศึกษานี้พบว่าสารสกัดหยาบใบฝรั่งมีฤทธิ์ในการยับยั้งแบคทีเรีย v. alginolyticus ได้สูงสุด จึงมีความเป็นไปได้ในการนำสารสกัดหยาบจากใบฝรั่งมาประยุกต์ใช้ในวงการเพาะเลี้ยงสัตว์น้ำต่อไป
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Li, Yuan, Benjamin Metcalf, Sopio Chochua та ін. Validation of β-lactam minimum inhibitory concentration predictions for pneumococcal isolates with newly encountered penicillin binding protein (PBP) sequences [Supporting data]. Centers for Disease Control and Prevention (U.S.), 2017. http://dx.doi.org/10.15620/cdc/147467.
Full textAbstract:
The datafiles, R scripts, MIC tables, and other files were used to evaluate the prediction performance of a penicillin-binding protein (PBP) typing system and two methods (Random Forest (RF) and Mode MIC (MM) previously developed by this research team. This data and these files support the finding of the paper "Validation of β-lactam minimum inhibitory concentration predictions for pneumococcal isolates with newly encountered penicillin binding protein (PBP) sequences" at https://doi.org/10.1186%2Fs12864-017-4017-7 or at https://stacks.cdc.gov/view/cdc/47684.
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ชวนชื่น, รุ่งทิพย์, พรเพ็ญ พัฒนโสภณ та ศุภชัย เนื้อนวลสุวรรณ. การเฝ้าระวังการดื้อต่อยาฆ่าเชื้อและยาปฏิชีวนะของ Salmonella spp. และ Escherichia coli ในสายพันธุ์ที่แยกได้ในประเทศไทย : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2006. https://doi.org/10.58837/chula.res.2006.87.
Full textAbstract:
แยก Salmonella enterica จำนวน 257 isolates และ Escherichia coli จำนวน 60 isolates จากตัวอย่างที่ได้จาก ไก่ สุกรและสิ่งแวดล้อมในฟาร์ม ทำการหาค่า minimal inhibitory concentration (MIC) ต่อยาปฏิชีวนะและยาฆ่าเชื้อ ได้แก่ ampicillin (AMP) chloramphenicol (CHP) ciprofloxacin (CIP) erythromycin (ERY) gentamycin (GEN) tetracycline (TET) trmethoprim (TRI) benzalkonium chloride (BKC) chlorhexidine (CHX), copper sulfate (CuSO4) และ zinc chloride (ZnCl2) พบว่า salmonella 162 isolates (63.04%) และ E. coli 50 isolates (83.33%) ดื้อต่อยาปฏิชีวนะอย่างน้อยหนึ่งชนิด ค่า MIC ต่อ BKC, CHX, CUSO4 และ ZnCl2 เกาะกันเป็นกลุ่มใหญ่ โดยเชื้อทั้งหมดนี้ไวต่อ cyclohexane และการเติม CCCP ไม่ทำให้ค่า MIC ต่อ BKC และ CHX เปลี่ยนแปลง เมื่อตรวจหาการปรากฏของ ยีน qacE และ qacE[triangle]1 ใน Salmonella ที่มีค่า MIC ต่อ BKC สูงสุดและต่ำสุดจำนวน 127 isolates ไม่พบว่ามีการปรากฏของยีน qacE และพบยีน qacE[triangle]1 ใน 38 isolates (29.92%) ที่อาจอยู่และไม่อยู่บน class I integron เชื้อที่มียีน qacE[triangle]1 ไม่มีค่า MIC ต่อ BKC และยาปฏิชีวนะสูงกว่าเชื้อที่ไม่มียีน qacE[triangle]1นอกจากนี้ Salmonella สามารถถ่ายทอดยีนดื้อยาปฏิชีวนะไปยังแบคทีเรียชนิดอื่นได้ แต่ไม่พบการถ่ายทอดยีนที่ควบคุมการลดความไวต่อ BKC และ CHX การสัมผัส BKC ในระดับต่ำให้ mutant ที่มีค่า MIC ต่อยาฆ่าเชื้อชนิดนี้สูงขึ้นและความไวต่อ CHP และ ERY ลดลง โดยที่ CCCP ทำให้ค่า MIC เปลี่ยนแปลง ผลการวิจัยครั้งนี้แสดงให้เห็นว่า Salmonella และ E. coli ยังไม่พัฒนาการดื้อต่อยาฆ่าเชื้อและโลหะหนักที่ทดสอบ หรือมีการพัฒนาในระดับที่ยังไม่สูงนัก ระบบ AcrAB และระบบ efflux systems อื่นๆ ที่ใช้พลังงานจาก Proton Motif Force (PMF) ไม่มีบทบาทสำคัญต่อการลดความไวต่อ BKC และ CHX การใช้ BKC ในระดับต่ำทำให้เชื้อพัฒนาการดื้อยาข้ามต่อยาปฏิชีวนะได้ ซึ่งสนับสนุนว่าควรมีการวางมาตรการควบคุมการใช้ยาฆ่าเชื้ออย่างสุขุมรอบคอบเช่นกัน
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ประภัสระกูล, ณุวีร์, เผด็จ ธรรมรักษ์ та ธงชัย เฉลิมชัยกิจ. ผลของสารเร่งการเจริญเติบโตต่อความไวรัสของเชื้ออี.โคไลในอุจจาระสุกรอนุบาลต่อยาต้านจุลชีพ. จุฬาลงกรณ์มหาวิทยาลัย, 2005. https://doi.org/10.58837/chula.res.2005.61.
Full textAbstract:
จากการทดลองของคณะผู้วิจัยพบว่าการใช้โคลิสตินและสารสมุนไพรเบอร์เบอร์รีนผสมอาหารเพื่อเร่งการเจริญเติบโตมีประสิทธิภาพในการควบคุมปริมาณเชื้ออี. โคไล ดีกว่าการใช้ฮาควินอล อย่างไรก็ตามเชื้อที่สามารถอยู่รอดหลังจากการได้รับสารต้านจุลชีพในระดับต่ำกว่าการรักษาอาจส่งผลให้เกิดการดื้อยาได้ การทดลองนี้มีวัตถุประสงค์เพื่อศึกษาเปรียบเทียบค่าความเข้มข้นที่ต่ำที่สุดของยาที่สามารถยับยั้งการเจริญเติบโตของเชื้อ(Minimal Inhibitory Concentration; MIC) ของเชื้ออี. โคไล จำนวน 78 เชื้อ ที่ได้จากการทดลองของคณะวิจัยเองการทดลองพบว่า เชื้ออี. โคไล ที่แยกได้ในสัปดาห์แรกก่อนให้สารต้านจุลชีพมีค่า MIC50 เท่ากับ 2 [mu]g/ml และหลังจากให้ยาติดต่อกับ 6 สัปดาห์ (ที่อายุ 10 สัปดาห์) ค่า MIC50 และ MIC90 ต่อโคลิสตินเพิ่มเป็น 8 และ16 [mu]g/ml ตามลำดับ ในขณะที่ไม่พบการเปลี่ยนแปลงของค่า MIC50ต่อโคลิสตินในเชื้ออี. โคไลที่แยกได้จากสุกรในกลุ่มที่ได้รับฮาควินอลและเบอร์เบอร์รีน ในกลุ่มสุกรที่ได้รับฮาควินอล เชื้ออี. โคไล มีค่า MIC50 ต่อยาฮาควินอล มีค่าเท่ากับ 8 [mu]g/ml ในสัปดาห์ที่ 4 และเพิ่มเป็น 16 [mu]g/ml ในสัปดาห์ที่ 6 และ 10 ในขณะที่เชื้ออี. โคไล ที่แยกได้จากสุกรที่ได้เบอร์เบอร์รีน มีค่า MIC50ต่อเบอร์เบอร์รีนมากกว่า 128 [mu]g/ml ตลอดการทดลอง นอกจากนี้ได้ทำการหาค่า MIC ของเชื้อ อี. โคไล ที่แยกได้ทั้งหมดต่อยาต้านจุลชีพอีกจำนวน 13 ชนิด พบว่าการให้สารเร่งการเจริญเติบโตทั้ง 3 ชนิด ไม่มีผลต่อการเพิ่มของระดับ MIC ต่อยาทั้ง 13 ชนิด และพบว่ายาต้านจุลชีพที่ยับยั้งการเจริญของเชื้ออี.โคไลที่มีช่วงค่าMIC50ที่มากกว่า 128 [mu]g/ml ดังนี้คือ เพนิซิลลิน อะม็อกซี่ซิลลิน นาลิดิซิกแอซิด ลินโคมัยซิน ไทโลซิน ไทอะมูลิน เตตราไซคลิน คลอเตตราไซคลิน ยาที่มีช่วงค่า MIC50 อยู่ในระดับสูง (32-128 [mu]g/ml) ได้แก่ สเตรปโตมัยซิน อีริโทรมัยซิน และด๊อกซี่ไซคลิน ซัลฟาเมทอกซาซอลร่วมกับไตเมทโทพริมมีค่า MIC50>8/152[mu]g/ml และเอนโรฟอกซาซินมีค่าMIC50เท่ากับ 16[mu]g/ml จากการศึกษาครั้งนี้พบว่าการใช้โคลิสตินและฮาควินอลผสมอาหารเป็นระยะเวลา 6 สัปดาห์ มีแนวโน้มทำให้ค่า MIC ของเชื้อ อี. โคไล เพิ่มขึ้น ซึ่งแสดงให้เห็นถึงผลของการใช้ยาปฏิชีวนะผสมอาหารในระดับต่ำกว่าระดับรักษาที่ส่งผลต่อภาวะการคัดเลือกเชื้อแบคทีเรียดื้อยาในลำไส้ และอาจมีผลต่อแนวโน้มการเพิ่มขึ้นของเชื้อที่ดื้อยาในตัวสัตว์และสิ่งแวดล้อมในระยะยาว
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Jorgensen, Frieda, John Rodgers, Daisy Duncan, Joanna Lawes, Charles Byrne, and Craig Swift. Levels and trends of antimicrobial resistance in Campylobacter spp. from chicken in the UK. Food Standards Agency, 2022. http://dx.doi.org/10.46756/sci.fsa.dud728.
Full textAbstract:
Campylobacter spp. are the most common bacterial cause of foodborne illness in the UK, with chicken considered to be the most important vehicle of transmission for this organism. It is estimated there are 500,000 cases of campylobacteriosis in the UK annually, with Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) accounting for approximately 91% and 8 % of infections, respectively. Although severe infection in humans is uncommon, treatment is seldom needed for human infection but usually involves the administration of a macrolide (e.g., azithromycin) or a fluoroquinolone (e.g., ciprofloxacin). An increased rate of resistance in Campylobacter in chicken to such antimicrobials could limit effective treatment options for human infections and it is therefore important to monitor changes in rates of resistance over time. In this report we analysed trends in antimicrobial resistance (AMR) in C. jejuni and C. coli isolated from chicken in the UK. The chicken samples were from chicken reared for meat (ie. broiler chicken as opposed to layer chicken (ie. egg-laying chicken)) and included chicken sampled at slaughterhouses as well as from retail stores in the UK. Datasets included AMR results from retail surveys of Campylobacter spp. on chicken sampled in the UK from various projects in the time period from 2001 to 2020. In the retail surveys, samples were obtained from stores including major and minor retail stores throughout the UK (in proportion to the population size of each nation) and Campylobacter spp. testing was performed using standard methods with the majority of isolates obtained from direct culture on standard media (mCCDA). Data from national scale surveys of broiler chicken, sampling caecal contents and carcase neckskins at slaughterhouses, undertaken by APHA in 2007/2008, and between 2012 and 2018 were also included in the study. In the APHA-led surveys, Campylobacter were isolated using standard culture methods (culture onto mCCDA) and antimicrobial susceptibility testing was performed by a standard microbroth dilution method to determine the minimum inhibitory concentration (MIC) of isolates. Care was taken when comparing data from different studies as there had been changes to the threshold used to determine if an isolate was susceptible or resistant to an antimicrobial in a small number of scenarios. Harmonised thresholds (using epidemiological cut-off (ECOFF) values) were employed to assess AMR with appropriate adjustments made where required to allow meaningful comparisons of resistance prevalence over time. Data from additional isolates where resistance to antimicrobials were predicted from genome sequence data were also considered.