Academic literature on the topic 'MinION sequencing'

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Journal articles on the topic "MinION sequencing"

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Batovska, Jana, Stacey E. Lynch, Brendan C. Rodoni, Tim I. Sawbridge, and Noel Oi Cogan. "Metagenomic arbovirus detection using MinION nanopore sequencing." Journal of Virological Methods 249 (June 12, 2017): 79–84. https://doi.org/10.5281/zenodo.13515287.

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(Uploaded by Plazi for the Bat Literature Project) With its small size and low cost, the hand-held MinION sequencer is a powerful tool for in-field surveillance. Using a metagenomic approach, it allows non-targeted detection of viruses in a sample within a few hours. This study aimed to determine the ability of the MinION to metagenomically detect and characterise a virus from an infected mosquito. RNA was extracted from an Aedes notoscriptus mosquito infected with Ross River virus (RRV), converted into cDNA and sequenced on the MinION. Bioinformatic analysis of the MinION reads led to detecti
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Batovska, Jana, Stacey E. Lynch, Brendan C. Rodoni, Tim I. Sawbridge, and Noel Oi Cogan. "Metagenomic arbovirus detection using MinION nanopore sequencing." Journal of Virological Methods 249 (June 7, 2017): 79–84. https://doi.org/10.5281/zenodo.13515287.

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(Uploaded by Plazi for the Bat Literature Project) With its small size and low cost, the hand-held MinION sequencer is a powerful tool for in-field surveillance. Using a metagenomic approach, it allows non-targeted detection of viruses in a sample within a few hours. This study aimed to determine the ability of the MinION to metagenomically detect and characterise a virus from an infected mosquito. RNA was extracted from an Aedes notoscriptus mosquito infected with Ross River virus (RRV), converted into cDNA and sequenced on the MinION. Bioinformatic analysis of the MinION reads led to detecti
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Batovska, Jana, Stacey E. Lynch, Brendan C. Rodoni, Tim I. Sawbridge, and Noel Oi Cogan. "Metagenomic arbovirus detection using MinION nanopore sequencing." Journal of Virological Methods 249 (July 3, 2017): 79–84. https://doi.org/10.5281/zenodo.13515287.

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(Uploaded by Plazi for the Bat Literature Project) With its small size and low cost, the hand-held MinION sequencer is a powerful tool for in-field surveillance. Using a metagenomic approach, it allows non-targeted detection of viruses in a sample within a few hours. This study aimed to determine the ability of the MinION to metagenomically detect and characterise a virus from an infected mosquito. RNA was extracted from an Aedes notoscriptus mosquito infected with Ross River virus (RRV), converted into cDNA and sequenced on the MinION. Bioinformatic analysis of the MinION reads led to detecti
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Batovska, Jana, Stacey E. Lynch, Brendan C. Rodoni, Tim I. Sawbridge, and Noel Oi Cogan. "Metagenomic arbovirus detection using MinION nanopore sequencing." Journal of Virological Methods 249 (July 10, 2017): 79–84. https://doi.org/10.5281/zenodo.13515287.

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(Uploaded by Plazi for the Bat Literature Project) With its small size and low cost, the hand-held MinION sequencer is a powerful tool for in-field surveillance. Using a metagenomic approach, it allows non-targeted detection of viruses in a sample within a few hours. This study aimed to determine the ability of the MinION to metagenomically detect and characterise a virus from an infected mosquito. RNA was extracted from an Aedes notoscriptus mosquito infected with Ross River virus (RRV), converted into cDNA and sequenced on the MinION. Bioinformatic analysis of the MinION reads led to detecti
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Lemon, Jamie K., Pavel P. Khil, Karen M. Frank, and John P. Dekker. "Rapid Nanopore Sequencing of Plasmids and Resistance Gene Detection in Clinical Isolates." Journal of Clinical Microbiology 55, no. 12 (2017): 3530–43. http://dx.doi.org/10.1128/jcm.01069-17.

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ABSTRACTRecent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality. Together, these improvements may permit real-time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates. In this study, we evaluated workflows and turnaround times for a benchtop long-read sequencing approach in the clinical microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer. We performed genomic and plasmid sequencing of three clinical isolates with both MinION and Illumina MiSeq, using different li
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Agakhanov, M. M., E. A. Grigoreva, E. K. Potokina, P. S. Ulianich, and Y. V. Ukhatova. "Genome assembly of Vitis rotundifolia Michx. using third-generation sequencing (Oxford Nanopore Technologies)." Proceedings on applied botany, genetics and breeding 182, no. 2 (2021): 63–71. http://dx.doi.org/10.30901/2227-8834-2021-2-63-71.

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The immune North American grapevine species Vitis rotundifolia Michaux (subgen. Muscadinia Planch.) is regarded as a potential donor of disease resistance genes, withstanding such dangerous diseases of grapes as powdery and downy mildews. The cultivar ‘Dixie’ is the only representative of this species preserved ex situ in Russia: it is maintained by the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in the orchards of its branch, Krymsk Experiment Breeding Station. Third-generation sequencing on the MinION platform was performed to obtain information on the primary structu
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Tafess, Ketema, Timothy Ting Leung Ng, Hiu Yin Lao, et al. "Targeted-Sequencing Workflows for Comprehensive Drug Resistance Profiling of Mycobacterium tuberculosis Cultures Using Two Commercial Sequencing Platforms: Comparison of Analytical and Diagnostic Performance, Turnaround Time, and Cost." Clinical Chemistry 66, no. 6 (2020): 809–20. http://dx.doi.org/10.1093/clinchem/hvaa092.

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Abstract Background The emergence of Mycobacterium tuberculosis with complex drug resistance profiles necessitates a rapid and comprehensive drug susceptibility test for guidance of patient treatment. We developed two targeted-sequencing workflows based on Illumina MiSeq and Nanopore MinION for the prediction of drug resistance in M. tuberculosis toward 12 antibiotics. Methods A total of 163 M. tuberculosis isolates collected from Hong Kong and Ethiopia were subjected to a multiplex PCR for simultaneous amplification of 19 drug resistance-associated genetic regions. The amplicons were then bar
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de Lannoy, Carlos, Dick de Ridder, and Judith Risse. "A sequencer coming of age: De novo genome assembly using MinION reads." F1000Research 6 (July 7, 2017): 1083. http://dx.doi.org/10.12688/f1000research.12012.1.

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Nanopore technology provides a novel approach to DNA sequencing that yields long, label-free reads of constant quality. The first commercial implementation of this approach, the MinION, has shown promise in various sequencing applications. This review gives an up-to-date overview of the MinION's utility as a de novo sequencing device. It is argued that the MinION may allow for portable and affordable de novo sequencing of even complex genomes in the near future, despite the currently error-prone nature of its reads. Through continuous updates to the MinION hardware and the development of new a
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de Lannoy, Carlos, Dick de Ridder, and Judith Risse. "The long reads ahead: de novo genome assembly using the MinION." F1000Research 6 (December 12, 2017): 1083. http://dx.doi.org/10.12688/f1000research.12012.2.

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Nanopore technology provides a novel approach to DNA sequencing that yields long, label-free reads of constant quality. The first commercial implementation of this approach, the MinION, has shown promise in various sequencing applications. This review gives an up-to-date overview of the MinION's utility as a de novo sequencing device. It is argued that the MinION may allow for portable and affordable de novo sequencing of even complex genomes in the near future, despite the currently error-prone nature of its reads. Through continuous updates to the MinION hardware and the development of new a
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Jaudou, Sandra, Mai-Lan Tran, Fabien Vorimore, Patrick Fach, and Sabine Delannoy. "Evaluation of high molecular weight DNA extraction methods for long-read sequencing of Shiga toxin-producing Escherichia coli." PLOS ONE 17, no. 7 (2022): e0270751. http://dx.doi.org/10.1371/journal.pone.0270751.

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Next generation sequencing has become essential for pathogen characterization and typing. The most popular second generation sequencing technique produces data of high quality with very low error rates and high depths. One major drawback of this technique is the short reads. Indeed, short-read sequencing data of Shiga toxin-producing Escherichia coli (STEC) are difficult to assemble because of the presence of numerous mobile genetic elements (MGEs), which contain repeated elements. The resulting draft assemblies are often highly fragmented, which results in a loss of information, especially co
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Dissertations / Theses on the topic "MinION sequencing"

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Sim, Justin. "The evaluation of in-field whole genome sequencing using the minION™ nanopore sequencer for forensic DNA applications." Thesis, Sim, Justin (2018) The evaluation of in-field whole genome sequencing using the minION™ nanopore sequencer for forensic DNA applications. Masters by Coursework thesis, Murdoch University, 2018. https://researchrepository.murdoch.edu.au/id/eprint/41414/.

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In-field whole genome sequencing has been made possible by the introduction of the MinION™ DNA sequencer by Oxford Nanopore Technologies. Current forensic DNA analysis requires samples to be sent to a dedicated lab offsite. Depending on the location this process can take time. The evaluation of alternatives such as field sequencing using the MinION™, with the possibility of providing access to results in a shorter time with lower costs, should be researched and validated. The potential applications of the MinION™ in forensic scenarios have not been studied partly due to the recent release of t
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Cain, Elizabeth. "Targeted STR and SNP in-field sequencing by Oxford Nanopore MinION™ for the identification of an individual in a military scenario." Thesis, Cain, Elizabeth (2019) Targeted STR and SNP in-field sequencing by Oxford Nanopore MinION™ for the identification of an individual in a military scenario. Masters by Coursework thesis, Murdoch University, 2019. https://researchrepository.murdoch.edu.au/id/eprint/49630/.

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The requirement for DNA evidence in forensics has increased, meaning the demand for DNA typing has also increased. Current analytical processes for DNA evidence are known to be costly and time-consuming and traditionally occur at a centralised laboratory which can impact on the amount of time from sample collection to DNA profile generation. Therefore, research has focused on creating technologies that are capable of in-field analysis. Oxford Nanopore Technologies developed MinION™, a portable, cost-effective nanopore sequencer that is capable of in-field analysis. The development of in-field
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Fruchard, Cécile. "Étude des chromosomes sexuels et du déterminisme du sexe chez les plantes : comparaison des systèmes Silene et Coccinia." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1108/document.

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Bien que les sexes séparés (dioecie) soient plus rares que chez les animaux, ∼15 600 espèces dioiques ont évolué chez les angiospermes (∼6% de l'ensemble des espèces). La manière dont le sexe de ces plantes est contrôlé est une question centrale de la biologie végétale, mais également de l'agronomie car de nombreuses plantes cultivées sont des plantes dioiques (∼20% des espèces cultivées) mais dont un seul sexe (généralement les femelles) présente un intérêt agronomique. Pourtant, seulement trois gènes du déterminisme du sexe ont été identifiés à ce jour chez les plantes dioiques, chez le kaki
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Ulrich, Kristina. "Molecular epidemiological study on Infectious Pancreatic Necrosis Virus isolates from aquafarms in Scotland over three decades." Thesis, University of Stirling, 2018. http://hdl.handle.net/1893/28340.

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Introduction: RNA viruses are economically important pathogens of fish, and among these viruses, infectious pancreatic necrosis virus (IPNV) is of particular concern for the aquaculture industry, especially for farmed rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). This non-enveloped aquatic virus, which was first isolated in the UK in 1971, belongs to the family of Birnaviridae and has a bi-segmented dsRNA genome of about 6kb. IPNV is classified in 6 genogroups with correspondence to 10 known serotypes and an additional proposed genogroup of marine aquabirnaviruses (MAB
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Ilango, Sankaralingam. "Computer-aided underground mining machine sequencing." Ohio : Ohio University, 1987. http://www.ohiolink.edu/etd/view.cgi?ohiou1183047349.

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Pelley, Charles W. "A study of sequencing strategy for steep, tabular, hardrock orebodies." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41746.

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This thesis reviews, analyses and classifies extraction methods and sequences used in steep, tabular orebodies in Ontario hardrock mines and how new bulk mining techniques have affected them.<br>The thesis examines, as a case study, the complexities of sequencing the extraction of the Hemlo orebody through three adjacent mines and details the planning and monitoring of extracting one section of the David Bell orebody. It examines the use of numerical modelling as a tool in extraction sequence planning and demonstrates how, in conjunction with an instrumentation program, the results assist succ
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Smoljanovic, Muñoz Milivoj Antonio. "Optimum sequencing of underground ore reserves for different mining systems." Tesis, Universidad de Chile, 2012. http://www.repositorio.uchile.cl/handle/2250/111985.

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Magíster en Minería<br>Ingeniero Civil de Minas<br>Currently, mine plans are optimized by using many criteria, such as profit, life of mine, concentration of some pollutants, mining costs, confidence level or mineral resources, while attending constraints related to production rates, plant capacities and grades. Whilst this approach is successful in terms of producing high value production schedules, it uses a static sequence of production units (for open pit and underground mine) and therefore the optimization is performed within the level of freedom left by the original opening schedule and
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Quan, Aaron. "Batch Sequencing Methods for Computer Experiments." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1401462464.

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Mousavi, Nogholi Amin Alah. "Optimisation of open pit mine block sequencing." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/86697/1/Amin%20Alah_Mousavi%20Nogholi_Thesis.pdf.

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This study presents a comprehensive mathematical model for open pit mine block sequencing problem which considers technical aspects of real-life mine operations. As the open pit block sequencing problem is an NP-hard, state-of-the-art heuristics algorithms, including constructive heuristic, local search, simulated annealing, and tabu search are developed and coded using MATLAB programming language. Computational experiments show that the proposed algorithms are satisfactory to solve industrial-scale instances. Numerical investigation and sensitivity analysis based on real-world data are also c
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桂宏胜 and Hongsheng Gui. "Data mining of post genome-wide association studies and next generation sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193431.

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Books on the topic "MinION sequencing"

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Soliman, Mohamed Y., and Ahmed Alzahabi. Optimization of Hydraulic Fracture Stages and Sequencing in Unconventional Formations. Taylor & Francis Group, 2018.

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Soliman, Mohamed Y., and Ahmed Alzahabi. Optimization of Hydraulic Fracture Stages and Sequencing in Unconventional Formations. Taylor & Francis Group, 2018.

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Soliman, Mohamed Y., and Ahmed Alzahabi. Optimization of Hydraulic Fracture Stages and Sequencing in Unconventional Formations. Taylor & Francis Group, 2021.

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Soliman, Mohamed Y., and Ahmed Alzahabi. Optimization of Hydraulic Fracture Stages and Sequencing in Unconventional Formations. Taylor & Francis Group, 2018.

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Optimization of Hydraulic Fracture Stages and Sequencing in Unconventional Formations. Taylor & Francis Group, 2018.

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Chaudhry, Bill, José Luis de la Pompa, and Nadia Mercader. The zebrafish as a model for cardiac development and regeneration. Edited by José Maria Pérez-Pomares, Robert G. Kelly, Maurice van den Hoff, et al. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780198757269.003.0029.

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The zebrafish has become an established laboratory model for developmental studies and is increasingly used to model aspects of human development and disease. However, reviewers and grant funding bodies continue to speculate on the utility of this Himalayan minnow. In this chapter we explain the similarities and differences between the heart from this distantly related vertebrate and the mammalian heart, in order to reveal the common fundamental processes and to prevent misleading extrapolations. We provide an overview of zebrafish including their husbandry, development, peculiarities of their
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Barker, Richard. The accelerating pace of biomedical advance. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780198737780.003.0002.

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Bioscience has progressed exponentially, in scientific advances and enabling technology. From quicker and much cheaper gene sequencing to the emergence of data-mining tools, the last 20 years has been unprecedented in exploitable advances brought by research. We have the tools and insights to trace disease from underlying genetics and epigenetics, through proteins that represent intervention options, to ways to create molecules, diagnostics, and devices based on those insights. The life sciences enterprise, once largely confined to Europe, the USA, and Japan, is now seeing major investment fro
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Book chapters on the topic "MinION sequencing"

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Runtuwene, Lucky R., Josef S. B. Tuda, Arthur E. Mongan, and Yutaka Suzuki. "On-Site MinION Sequencing." In Single Molecule and Single Cell Sequencing. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6037-4_10.

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Iralu, Nulevino, Dasari Meghanath, Sumiah Wani, Sahar Saleem, and Aflaq Hamid. "Preparation of Sequencing Library for Oxford Nanopore Minion Sequencing." In Springer Protocols Handbooks. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4390-7_32.

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Karamitros, Timokratis, and Gkikas Magiorkinis. "Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7514-3_4.

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Suzuki, Yasuyuki, and Shuang Liu. "Bulk RNA-seq Assessment of Murine Spleen Using a Portable MinION Sequencing Device." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3682-4_30.

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Yan, Lifang, Alejandro Banda, and Leyi Wang. "Whole Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION." In Springer Protocols Handbooks. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2091-5_22.

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Kumar, Anil, Max M. Häggblom, and Lee J. Kerkhof. "A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes Using the Oxford Nanopore MinION." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-4192-7_2.

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Singh, Roshan Kumar, Anurag Panchal, Mehanathan Muthamilarasan, and Manoj Prasad. "Genome Sequencing Efforts in Minor Millets: Current Knowledge and Emerging Insights." In Minor Millets. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-4265-6_11.

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Qian, Yu, Kang Zhang, and Wei Lai. "Constraint-Based Graph Clustering through Node Sequencing and Partitioning." In Advances in Knowledge Discovery and Data Mining. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-540-24775-3_7.

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A., Salim, Amjesh R., and Vinod Chandra S.S. "An Improved Algorithm for MicroRNA Profiling from Next Generation Sequencing Data." In Data Mining and Big Data. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-40973-3_4.

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Zhang, Qian, Junping Zhang, and Chenghai Xue. "Measuring Reproducibility of High-Throughput Deep-Sequencing Experiments Based on Self-adaptive Mixture Copula." In Advances in Knowledge Discovery and Data Mining. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37453-1_25.

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Conference papers on the topic "MinION sequencing"

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Arrones, Andrea, Adriel Latorre, Cristina Vilanova, et al. "INTRODUCTION TO ADVANCED SEQUENCING TECHNOLOGIES FOR UNDERGRADUATE STUDENTS IN GENETICS: MINION REAL-TIME SEQUENCING." In 16th International Technology, Education and Development Conference. IATED, 2022. http://dx.doi.org/10.21125/inted.2022.2333.

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Kchouk, Mehdi, and Mourad Elloumi. "Hybrid error correction approach and de novo assembly for minion sequencing long reads." In 2016 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2016. http://dx.doi.org/10.1109/bibm.2016.7822504.

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Kchouk, Mehdi, and Mourad Elloumi. "Error correction and DeNovo genome Assembly for the MinIon sequencing reads mixing Illumina short reads." In 2015 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2015. http://dx.doi.org/10.1109/bibm.2015.7359962.

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Matos, William Latosinski, Dariane Castro Pereira, Patricia Orlandi Barth, et al. "Evaluation of extraction methods of high molecular weight DNA to reconstruct the circular genome of Enterobacterales using Oxford Nanopore Sequencing technology." In Resumos do 56º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2024. https://doi.org/10.5327/1516-3180.142s1.13081.

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Objective: The Oxford Nanopore Technologies (ONT) enables the identification of resistance genes and tracking of mobile genetic elements in clinical isolates. However, the quality and molecular weight of genomic DNA (gDNA) are vital for successful ONT sequencing, making DNA isolation a key step. The objective of his work was to compare two commercial DNA extraction kits in carbapenem-resistant enterobacterales (CRE) strains to evaluate their effectiveness in preserving gDNA integrity and purity for sequencing analysis and their impact on sequencing outcomes. Method: Sixteen bacterial isolates
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De Block, T., I. De Baetselier, S. Abdellati, et al. "P207 Evaluation of Oxford Nanopore MinION sequencing to predict antimicrobial resistance profiles in clinical N. gonorrhoeae strains." In Abstracts for the STI & HIV World Congress, July 14–17 2021. BMJ Publishing Group Ltd, 2021. http://dx.doi.org/10.1136/sextrans-2021-sti.296.

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Johnson, Jack L., Jose Garcia-Bravo, Pawan Panwar, and Paul Michael. "Strategies to Minimize Data Sample Size for Regression-Based Pump/Motor Models." In SICFP’21 The 17:th Scandinavian International Conference on Fluid Power. Linköping University Electronic Press, 2021. http://dx.doi.org/10.3384/ecp182p134.

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This work presents an analysis for tracking the evolution of regression coefficients and the Root-Mean-Square of their residuals on a test dataset for a hydraulic pump. The method starts by iteratively regressing data points that are undergoing sequencing by adding one new data sample at a time, then regressing with each iteration. This process was named Progressively Sequenced Regression Analysis, shortened to “PSR analysis” in this paper. The motivating and guiding postulate of PSR analysis is based on the belief that a plateau of the regression coefficients and statistical figures of merit
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Xiao, Yiou, Kishan G. Mehrotra, Damian G. Allis, and Phillip N. Borer. "A fast sorting algorithm for aptamer identification using deep sequencing." In 2014 IEEE/ACM International Conference on Advances in Social Networks Analysis and Mining (ASONAM). IEEE, 2014. http://dx.doi.org/10.1109/asonam.2014.6921671.

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Warnke, Julia, and Hesham Ali. "A Tolerance Graph Approach for Domain-Specific Assembly of Next Generation Sequencing Data." In 2013 IEEE 13th International Conference on Data Mining Workshops (ICDMW). IEEE, 2013. http://dx.doi.org/10.1109/icdmw.2013.105.

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Gillard, Xavier, and Pierre Schaus. "Large Neighborhood Search with Decision Diagrams." In Thirty-First International Joint Conference on Artificial Intelligence {IJCAI-22}. International Joint Conferences on Artificial Intelligence Organization, 2022. http://dx.doi.org/10.24963/ijcai.2022/659.

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Local search is a popular technique to solve combinatorial optimization problems efficiently. To escape local minima one generally uses metaheuristics or try to design large neighborhoods around the current best solution. A somewhat more black box approach consists in using an optimization solver to explore a large neighborhood. This is the large-neighborhood search (LNS) idea that we reuse in this work. We introduce a generic neighborhood exploration algorithm based on restricted decision diagrams (DD) constructed from the current best solution. We experiment DD-LNS on two sequencing problems
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Medina, Rafael Sales, Ana Paula Couto Da Silva, and Fabricio Murai. "SEMPLICe: Um Modelo Sequencial de Proficiência em Comunidades Online para Aprendizado de Idioma ∗." In VIII Brazilian Workshop on Social Network Analysis and Mining. Sociedade Brasileira de Computação - SBC, 2019. http://dx.doi.org/10.5753/brasnam.2019.6548.

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O Reddit é uma rede social online em que usuários interessados em um mesmo tópico interagem uns com os outros em subreddits. Subreddits para aprendizado de idioma vem atraindo usuários de diferentes nı́veis de pro- ficiência a cada ano, buscando melhorar o aprendizado. Em particular, no subreddit German, os usuários são aconselhados a informar seu nı́vel de pro- ficiência ao escrever um post. Contudo, apenas 20% dos posts possuem tais tags. Abordamos aqui o problema de classificar a proficiência dos usuários a partir de suas publicações. Conduzimos uma série de experimentos que demonstram que
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Reports on the topic "MinION sequencing"

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Cytryn, Eddie, Mark R. Liles, and Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7598174.bard.

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Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed
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Rodriguez Muxica, Natalia. Open configuration options Bioinformatics for Researchers in Life Sciences: Tools and Learning Resources. Inter-American Development Bank, 2022. http://dx.doi.org/10.18235/0003982.

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The COVID-19 pandemic has shown that bioinformatics--a multidisciplinary field that combines biological knowledge with computer programming concerned with the acquisition, storage, analysis, and dissemination of biological data--has a fundamental role in scientific research strategies in all disciplines involved in fighting the virus and its variants. It aids in sequencing and annotating genomes and their observed mutations; analyzing gene and protein expression; simulation and modeling of DNA, RNA, proteins and biomolecular interactions; and mining of biological literature, among many other c
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Ghanim, Murad, Joe Cicero, Judith K. Brown, and Henryk Czosnek. Dissection of Whitefly-geminivirus Interactions at the Transcriptomic, Proteomic and Cellular Levels. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592654.bard.

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Our project focuses on gene expression and proteomics of the whitefly Bemisia tabaci (Gennadius) species complex in relation to the internal anatomy and localization of expressed genes and virions in the whitefly vector, which poses a major constraint to vegetable and fiber production in Israel and the USA. While many biological parameters are known for begomovirus transmission, nothing is known about vector proteins involved in the specific interactions between begomoviruses and their whitefly vectors. Identifying such proteins is expected to lead to the design of novel control methods that i
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Weller, Joel I., Derek M. Bickhart, Micha Ron, Eyal Seroussi, George Liu, and George R. Wiggans. Determination of actual polymorphisms responsible for economic trait variation in dairy cattle. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600017.bard.

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The project’s general objectives were to determine specific polymorphisms at the DNA level responsible for observed quantitative trait loci (QTLs) and to estimate their effects, frequencies, and selection potential in the Holstein dairy cattle breed. The specific objectives were to (1) localize the causative polymorphisms to small chromosomal segments based on analysis of 52 U.S. Holstein bulls each with at least 100 sons with high-reliability genetic evaluations using the a posteriori granddaughter design; (2) sequence the complete genomes of at least 40 of those bulls to 20 coverage; (3) de
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