Academic literature on the topic 'MIP [Moleculary imprinted polymer]'
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Journal articles on the topic "MIP [Moleculary imprinted polymer]"
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Dong, Hong Xing, Qiu Li Jiang, Fei Tong, Zhen Xing Wang, and Jin Yong Tang. "Preparation of Imprinted Polymer with D-Phenylalanin on Silica Surface." Key Engineering Materials 419-420 (October 2009): 541–44. http://dx.doi.org/10.4028/www.scientific.net/kem.419-420.541.
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Silica gel was modified with polymerizable double bonds on the surface. Then the molecular imprinting polymer imprinted with D-Phenylalanin was grafted on the surface of modified silica gel. The molecularly imprinted polymer (MIP) based on the surface of silica gel was characterized by IR and scanning electron microscopy (SEM). The adsorption property of D-Phenylalanin by MIP was mearsured. The MIP with the combined functional monomers exhibited better adsorption properties and selectivity compared with the corresponding non-imprinted polymers or MIP with the single functional monomer. The material could be used in separation research field, such as packing material of high performance liquid chromatography, solid-phase extraction and so on.
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Wolska, Joanna, and Nasim Jalilnejad Falizi. "Membrane Emulsification Process as a Method for Obtaining Molecularly Imprinted Polymers." Polymers 13, no. 16 (August 23, 2021): 2830. http://dx.doi.org/10.3390/polym13162830.
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The membrane emulsification process (ME) using a metallic membrane was the first stage for preparing a spherical and monodisperse thermoresponsive molecularly imprinted polymer (TSMIP). In the second step of the preparation, after the ME process, the emulsion of monomers was then polymerized. Additionally, the synthesized TSMIP was fabricated using as a functional monomer N-isopropylacrylamide, which is thermosensitive. This special type of polymer was obtained for the recognition and determination of trace bisphenol A (BPA) in aqueous media. Two types of molecularly imprinted polymers (MIPs) were synthesized using amounts of BPA of 5 wt.% (MIP-2) and 7 wt.% (MIP-1) in the reaction mixtures. Additionally, a non-imprinted polymer (NIP) was also synthesized. Polymer MIP-2 showed thermocontrolled recognition for imprinted molecules and a higher binding capacity than its corresponding non-imprinted polymer and higher than other molecularly imprinted polymer (MIP-1). The best condition for the sorption process was at a temperature of 35 °C, that is, at a temperature close to the phase transition value for poly(N-isopropylacrylamide). Under these conditions, the highest levels of BPA removal from water were achieved and the highest adsorption capacity of MIP-2 was about 0.5 mmol g−1 (about 114.1 mg g−1) and was approximately 20% higher than for MIP-1 and NIP. It was also observed that during the kinetic studies, under these temperature conditions, MIP-2 sorbed BPA faster and with greater efficiency than its non-imprinted analogue.
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Roland, Rachel Marcella, and Showkat Ahmad Bhawani. "Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Piperine: Extraction of Piperine from Spiked Urine." Journal of Analytical Methods in Chemistry 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/5671507.
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Molecularly imprinted polymer (MIP) microspheres for Piperine were synthesized by precipitation polymerization with a noncovalent approach. In this research Piperine was used as a template, acrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and 2,2′-azobisisobutyronitrile (AIBN) as an initiator and acetonitrile as a solvent. The imprinted and nonimprinted polymer particles were characterized by using Fourier transform infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The synthesized polymer particles were further evaluated for their rebinding efficiency by batch binding assay. The highly selected imprinted polymer for Piperine was MIP 3 with a composition (molar ratio) of 0.5 : 3 : 8, template : monomer : cross-linker, respectively. The MIP 3 exhibits highest binding capacity (84.94%) as compared to other imprinted and nonimprinted polymers. The extraction efficiency of highly selected imprinted polymer of Piperine from spiked urine was above 80%.
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Hasanah, Aliya Nur, Nisa Safitri, Aulia Zulfa, Neli Neli, and Driyanti Rahayu. "Factors Affecting Preparation of Molecularly Imprinted Polymer and Methods on Finding Template-Monomer Interaction as the Key of Selective Properties of the Materials." Molecules 26, no. 18 (September 16, 2021): 5612. http://dx.doi.org/10.3390/molecules26185612.
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Molecular imprinting is a technique for creating artificial recognition sites on polymer matrices that complement the template in terms of size, shape, and spatial arrangement of functional groups. The main advantage of Molecularly Imprinted Polymers (MIP) as the polymer for use with a molecular imprinting technique is that they have high selectivity and affinity for the target molecules used in the molding process. The components of a Molecularly Imprinted Polymer are template, functional monomer, cross-linker, solvent, and initiator. Many things determine the success of a Molecularly Imprinted Polymer, but the Molecularly Imprinted Polymer component and the interaction between template-monomers are the most critical factors. This review will discuss how to find the interaction between template and monomer in Molecularly Imprinted Polymer before polymerization and after polymerization and choose the suitable component for MIP development. Computer simulation, UV-Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Proton-Nuclear Magnetic Resonance (1H-NMR) are generally used to determine the type and strength of intermolecular interaction on pre-polymerization stage. In turn, Suspended State Saturation Transfer Difference High Resolution/Magic Angle Spinning (STD HR/MAS) NMR, Raman Spectroscopy, and Surface-Enhanced Raman Scattering (SERS) and Fluorescence Spectroscopy are used to detect chemical interaction after polymerization. Hydrogen bonding is the type of interaction that is becoming a focus to find on all methods as this interaction strongly contributes to the affinity of molecularly imprinted polymers (MIPs).
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Huang, Chuixiu, and Xiantao Shen. "Janus molecularly imprinted polymer particles." Chem. Commun. 50, no. 20 (2014): 2646–49. http://dx.doi.org/10.1039/c3cc49586h.
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Janus MIP particles were synthesized via a wax–water Pickering emulsion. The MIP microtransporter concept provided the Janus MIP particles with attractive capabilities for autonomous binding and controlled drug delivery.
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Tadi, Kiran Kumar, and Ramani V. Motghare. "Synthesis and Evaluation of Molecularly Imprinted Polymer for Oxalic Acid." Advanced Materials Research 622-623 (December 2012): 229–35. http://dx.doi.org/10.4028/www.scientific.net/amr.622-623.229.
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Molecular imprinting technology is a convenient approach for preparing synthetic receptors that possesses user defined recognition properties. Oxalic acid imprinted bulk polymer was synthesized by thermal initiated free radical co-polymerization of oxalic acid (template) with two different functional monomers (acrylamide and methacrylic acid) and ethylene glycol dimethacrylate as crosslinker, using acetonitrile (porogen) as solvent. Scanning electron microscopy and FT-IR spectra confirmed the formation of molecularly imprinted polymer (MIP) with acrylamide. The synthesized MIP(ACR)efficiently adsorbed oxalic acid from aqueous solutions. The binding parameters of molecularly imprinted polymer and non-imprinted polymer were compared by Langmuir-Freundlich adsorption (LF) isotherm.
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Derz, Wiebke, Melita Fleischmann, and Paul W. Elsinghorst. "Guiding Molecularly Imprinted Polymer Design by Pharmacophore Modeling." Molecules 26, no. 16 (August 23, 2021): 5101. http://dx.doi.org/10.3390/molecules26165101.
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Molecularly imprinted polymers (MIP) combine the selectivity of immunoaffinity chromatography with the robustness of common solid-phase extraction in what is referred to as molecularly imprinted solid-phase extraction (MISPE). This contribution shows how MIP design may be guided by pharmacophore modeling for the example of citrinin, which is an emerging mycotoxin from cereals. The obtained pharmacophore model allowed searching public databases for a set of citrinin-mimicking molecular surrogates. Imprinted and non-imprinted polymers were subsequently obtained through bulk and core-shell polymerization in the presence of these surrogates. Evaluation of their binding ability for citrinin and structurally related ochratoxin A revealed a promising MIP derived from rhodizonic acid. A protocol for MISPE of citrinin from cereals was subsequently developed and compared to immunoaffinity chromatography with respect to clean-up efficiency and recovery.
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Baek, In-Hyuk, Hyung-Seop Han, Seungyun Baik, Volkhard Helms, and Youngjun Kim. "Detection of Acidic Pharmaceutical Compounds Using Virus-Based Molecularly Imprinted Polymers." Polymers 10, no. 9 (September 1, 2018): 974. http://dx.doi.org/10.3390/polym10090974.
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Molecularly imprinted polymers (MIPs) have proven to be particularly effective chemical probes for the molecular recognition of proteins, DNA, and viruses. Here, we started from a filamentous bacteriophage to synthesize a multi-functionalized MIP for detecting the acidic pharmaceutic clofibric acid (CA) as a chemical pollutant. Adsorption and quartz crystal microbalance with dissipation monitoring experiments showed that the phage-functionalized MIP had a good binding affinity for CA, compared with the non-imprinted polymer and MIP. In addition, the reusability of the phage-functionalized MIP was demonstrated for at least five repeated cycles, without significant loss in the binding activity. The results indicate that the exposed amino acids of the phage, together with the polymer matrix, create functional binding cavities that provide higher affinity to acidic pharmaceutical compounds.
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Aprilia, Suci. "PENGARUH MILLING TERHADAP KARAKTERISTIK MOLECULARLY IMPRINTED POLYMER KARBARIL (C12H11NO2)." JOURNAL ONLINE OF PHYSICS 5, no. 2 (July 25, 2020): 1–5. http://dx.doi.org/10.22437/jop.v5i2.9430.
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Molecularly Imprinted Polymer (MIP) has been synthesized with carbaryl used as a template and forms complex bonds with Methacrylic Acid (MAA)through hydrogen bonds, followed by a cross-linking process using Ethylene Glycol Dimethacrylate (EGDMA) cross-linkers. The polymerization process begins with the thermal decomposition of Benzoyl Peroxide (BPO) as an initiator. Non-Imprinted Polymer (NIP) as a control polymer has also been synthesized using a similar procedure without using carbaryl templates. In this study, two variations of carbaryl are used, which are not milled carbamates, and carbaryl has been milled using High Energy Milling (HEM). The FTIR study was carried out to investigate the presence of carbaryl in polymers, MIP, and NIP. The spectrum shows that the concentration of carbaryl compound decreases after the extraction process. This result was also confirmed by the increase in the value of the percentage of transmittance in the MIP, especially the nano carbaryl MIP. This result is supported by the X-RD results, which showed a decrease in the size of the crystals in the carbaryl MIP from 10.07 Å while the nano carbaryl MIP was 9.16 Å.
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Guo, Yan, Leilei Kang, Shaona Chen, and Xin Li. "High performance surface-enhanced Raman scattering from molecular imprinting polymer capsulated silver spheres." Physical Chemistry Chemical Physics 17, no. 33 (2015): 21343–47. http://dx.doi.org/10.1039/c5cp00206k.
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Driven by the ultrasensitivity of the surface-enhanced Raman scattering (SERS) technique and the directive selection of molecular imprinting polymers (MIPs), core–shell silver-molecularly imprinted polymer (Ag@MIP) hybrid structure was synthesized to serve as a novel SERS platform.
Dissertations / Theses on the topic "MIP [Moleculary imprinted polymer]"
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Wagner, Sabine. "Sensory molecularly imprinted polymer (MIP) coatings for nanoparticle- and fiber optic-based assays." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/19808.
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Für den Nachweis dieser Schadstoffe in niedrigen Konzentrationsbereichen sind schnelle und empfindliche Analysemethoden erforderlich. Molekular geprägte Polymere (MIPs) wurden als synthetische Materialien entwickelt, um die molekulare Erkennung von natürlichen Rezeptoren nachzuahmen, aufgrund ihrer Fähigkeit, selektiv eine Vielzahl von Analyten zu erkennen, ihre Stabilität und ihrer einfachen Herstellung. Sie sind zunehmend in der chemischen Sensorik als Rezeptormaterial für den Nachweis bestimmter Analyten bei niedrigen Konzentrationen zu finden, insbesondere in Kombination mit Fluoreszenz aufgrund dessen hoher Empfindlichkeit.
Ziel dieser Arbeit war die Entwicklung von optischen Sensormaterialien unter Verwendung von MIPs als Erkennungselemente im Zusammenhang mit Fluoreszenz zum sensitiven Nachweis von Herbiziden und Antibiotika in Wasser- und Lebensmittelproben and deren Kombination mit verschiedenen Vorrichtungsformaten für die zukünftige Detektion einer breiten Palette von wichtigen Analyten.For the detection of these contaminants in low concentration ranges fast and sensitive analytical tools are required. Molecularly imprinted polymers (MIPs) have been used as synthetic materials mimicking molecular recognition by natural receptors due to their ability to recognize selectively a wide range of analytes, their stability and ease of synthesis. They have gained more and more attention in chemical sensing as receptor material for the detection of suitable groups of analytes at low concentrations especially in combination with fluorescence due to the latter’s high sensitivity. This work aimed the development of optical sensor materials using MIPs as recognition elements connected with fluorescence for the sensitive detection of herbicides and antibiotics in water and food samples and their combination with various device formats for the future detection of a wide range of analytes.
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Tsai, Mei-Hsuan. "Boron containing molecular imprinted polymer (MIP) templates from symmetric and asymmetric diboration of olefins and other boron containing functional polymers." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608235.
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Bitar, Manal. "Design d'un iprodione-MIP (molecularly imprinted polymer) : application à la pré-concentration des fongicides dans le vin." Thesis, Dijon, 2014. http://www.theses.fr/2014DIJOS066/document.
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Les travaux de thèse ont porté sur la synthèse d’un MIP (polymère à empreintes moléculaires) spécifique d’un fongicide se trouvant dans la majorité des vins français : l’iprodione. Pour extraire l’iprodione à partir du vin, un challenge important se présentait : le milieu hydroalcoolique est un solvant dipolaire alors que l’interaction du MIP avec l’iprodione se base sur des interactions dipôle-dipôle. Les premières études d’extraction de l’iprodione ont été faites sur un MIP obtenu par polymérisation en masse à partir du méthacrylamide et de l'éthylène glycol diméthacrylate (EGDMA). Le MIP était plus efficace que le NIP (polymère non imprimé) dans des solutions hydroalcooliques ce qui vérifie que l’impression moléculaire était réussie. Un plan d’expériences 23 a été appliqué pour étudier l’influence de 3 facteurs de synthèse de MIP (la méthode de polymérisation, la nature de l'agent réticulant et le type de monomère fonctionnel) sur les propriétés de reconnaissance des polymères vis-à-vis de l’iprodione. 8 MIP et 8 NIP ont été ainsi synthétisés. Les isothermes d'adsorption de l'eau et de l’iprodione par les MIPs et les NIPs ont été déterminées. Des différences significatives entre les polymères ont été mises en évidences impliquant une relation entre l’adsorption de l’eau et l’adsorption de l’iprodione. La formation des empreintes moléculaires au sein des MIP a été démontrée par plusieurs techniques comme la calorimétrie différentielle à balayage et la résonance magnétique nucléaire. Les meilleures propriétés de reconnaissance de l’iprodione sont obtenues avec le MIP synthétisé par précipitation à partir du méthacrylamide comme monomère fonctionnel et de l’EGDMA comme réticulant. Ce MIP a été utilisé en extraction en phase solide (SPE) pour la pré-concentration de l’iprodione dans un vin blanc avec un facteur de pré-concentration égal à 6 et sa sélectivité par rapport à deux fongicides : le pyriméthanil et le procymidone a été démontréeThe aim of this study was the synthesis of a MIP (molecularly imprinted polymer) specific for a fungicide that is found in the majority of the french wine: iprodione. The challenge for extracting iprodione from wine medium was that the hydoralcoholic solution is a dipolar solvent, whereas the interaction between the MIP and iprodione is based on dipolar interactions. The first extraction study of iprodione was made on a MIP synthesized by bulk polymerization using methacrylamide and ethylene glycol dimethacrylate (EGDMA). The MIP was found to be more efficient than the NIP (non-imprinted polymer) in hydroalcoholic solutions which demonstrates that the molecular imprinting was successful. Then we synthesized 8 MIPs and 8 NIPs following an 23 experimental design in order to study the influence of three synthesis factors (the polymerization method, the nature of the crosslinker and the type of the functional monomer) on the iprodione recognition properties of the polymers. The water and the iprodione adsorption isotherms for MIPs and NIPs were determined. The result showed significant differences between the polymers involving a relationship between the water adsorption and the adsorption of iprodione. The molecular imprinting has been demonstrated by several techniques such as the differential scanning calorimetry and the nuclear magnetic resonance. The best recognition properties of iprodione are obtained with the MIP which was synthesized by precipitation polymerization using methacrylamide as functional momonomer and EGDMA as crosslinker. This MIP was used in solid phase extraction (SPE) for pre-concentration of iprodione in a white wine with a pre-concentration factor of 6. Its selectivity versus two fungicides: procymidone and pyrimethanil has been demonstrated
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Le, Moullec Sophie. "Développement de polymères à empreintes moléculaires pour l'extraction sélective de produits de dégradation de neurotoxiques organophosphorés de matrices complexes." Paris 6, 2007. http://www.theses.fr/2007PA066462.
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Ce travail a eu pour objectif de développer des polymères à empreintes moléculaires (MIP) afin d’extraire sélectivement les alkyl alkylphosphonates de matrices complexes. Ces MIP contiennent des cavités conduisant à la reconnaissance d’une molécule dite « empreinte ». Plusieurs MIP ont été synthétisés utilisant le pinacolyl méthylphosphonate comme empreinte puis caractérisés. Ces MIP ont ensuite été utilisé pour le traitement de matrices complexes mettant en évidence leur aptitude à isoler les molécules cibles des interférents. La percolation d’eau sur MIP n’étant pas possible, une méthode de SPE a été développée afin de procéder au changement de solvant. La dernière partie de ce travail a porté sur la compréhension des interactions empreinte-monomères grâce à l’utilisation de la modélisation moléculaire et de la RMN. Les résultats obtenus ont permis de mieux appréhender les mécanismes de formation des cavités et mis en évidence les monomères interagissant fortement avec l’empreinte.
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Xu, Jingjing. "Solid-phase synthesis of molecularly imprinted polymer nanoparticles for protein recognition." Thesis, Compiègne, 2017. http://www.theses.fr/2017COMP2349/document.
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Cette thèse décrit la synthèse de nanoparticules de polymères à empreintes moléculaires (MIP, de l’anglais molecularly imprinted polymer) pour la reconnaissance de protéines, par une approche de synthèse en phase solide. Les polymères à empreintes moléculaires sont des récepteurs biomimétiques synthétisés sur mesure par un processus de nanomoulage du polymère autour de la molécule unique. Ils possèdent ainsi des cavités de reconnaissance spécifiques pour leur molécule cible. La technique de l'impression moléculaire pour les petites molécules cibles est bien établie, alors que l'impression de protéines reste encore un défi en raison de la flexibilité et complexité de leur structure native et de leurs nombreux sites fonctionnels, mais aussi en raison de leur faible stabilité dans des conditions inhabituelles. Par conséquent, une approche de synthèse en phase solide a été développée ici où la protéine est immobilisée sur un support avant la synthèse de nanoparticules hydrosolubles de MIP par polymérisation radicalaire. Les MIPs obtenus ont des affinités comparables à celles des anticorps, et des réactivités croisées faibles. Ils possèdent des avantages tels qu'une stabilité meilleure, un coût plus faible et peuvent potentiellement être régénérés et réutilisés, devenant ainsi des alternatives prometteuses aux anticorps naturels. Nous avons fabriqué des MIPs contre des protéases à sérine, telles la trypsine et la kallikréine, mais aussi contre un épitope peptidique de la protéine gp41 du VIH. Des nanogels de MIP thermosensibles ont été synthétisés dans un réacteur sous la forme d’une colonne thermostatée ou une boîte de Pétri, par polymérisation radicalaire initiée par voie thermique ou photochimique. Un simple changement de la température permet de libérer les MIPs de la protéine immobilisée. Ces MIPs sont hydrosolubles en fonction de la température et ont un diamètre inférieur à 100 nm. Leur affinité pour leur cible est élevée, avec un Kd du nano ou picomolaire. Ces 'anticorps synthétiques' ont été appliqués dans des tests d'adsorption sur microbalance à cristal de quartz, mais également comme 'chaperons synthétiques'. Des études préliminaires de la protection des protéines d'une dénaturation thermique ou par un pH défavorable ont été effectuées. L'utilisation d'un iniferter pour initier la photopolymérisation vivante du MIP a permis de synthétiser des nanogels de type core-shell. En introduisant des marqueurs fluorescents dans les MIPs, les tests d’immunoessai dans des fluides biologiques ont été démontrés, ce qui indique le grand potentiel de ces MIPs dans le diagnostic clinique. En conclusion, nous avons développé une nouvelle approche de synthèse de nanoparticules de MIP hydrosoluble ayant une haute affinité pour une protéine, utilisables à la place des anticorps dans des applications dans le monde réel tel que la détection de protéines biomarqueurs dans des échantillons complexes, et potentiellement comme principe actif in vivoThis thesis describes the synthesis, by a solid-phase synthesis approach, of nanoparticles of molecularly imprinted polymers (MIPs) for the recognition of proteins. Molecularly imprinted polymers are biomimetic receptors synthesized by a nanomolding process of the polymer around single molecules. They therefore possess specific recognition cavities for their target molecule. The technique of molecular imprinting for small target molecules is well established, while protein imprinting remains a challenge due to the flexibility and complexity of their native structure and functional sites, but also because of their low stability under unusual conditions. Therefore, a solid-phase synthesis approach has been developed where the protein is immobilized on a support before the synthesis of water-soluble MIP nanogel particles by radical polymerization. The MIPs obtained have affinities comparable to those of antibodies, and low cross-reactivities. They have advantages such as better stability, lower cost, and can potentially be regenerated and reused, thus becoming promising alternatives to real antibodies. We have synthesized MIPs against serine proteases such as trypsin, and kallikrein, but also against a peptide epitope of the HIV gp41 protein. Thermosensitive MIP nanogels were synthesized in a thermostated column-type reactor or a petri dish, by thermally or photo-initiated radical polymerization. Their thermosensitivity allows the MIPs to be released from the immobilized protein by a simple temperature change. They are water-soluble as a function of temperature and have a diameter of less than 100 nm. Their affinity for their target is strong, with a Kd in the nano or picomolar range. These 'synthetic antibodies' have been applied in binding assays with quartz crystal microbalance, but also as 'synthetic chaperones'. Preliminary studies of the protection of proteins from thermal denaturation or from denaturation by an unfavorable pH have been carried out. The use of an iniferter to initiate the living photopolymerization of MIP made it possible to synthesize nanogels of core-shell type. By introducing fluorescent markers into MIPs, immunoassay applications in biological fluids have been demonstrated, indicating the great potential of these MIPs in clinical diagnostics. In conclusion, we have developed a novel approach to the synthesis of soluble MIP nanoparticles having high affinity for a protein, usable in place of antibodies in real world applications such as the detection of biomarker proteins in complex samples, and potentially as an active principle in vivo
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Santos, Wilney de Jesus Rodrigues. "Nanoreatores biomimeticos a peroxidase baseados em MIP : uma estrategia promissora para determinação de compostos fenolicos." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248404.
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Orientador: Lauro Tatsuo KubotaTese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica
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Resumo: O presente trabalho descreve as aplicações de nanoreatores biomiméticos à peroxidase baseados em MIP ("Molecularly Imprinted Polymers") como uma ferramenta promissora para determinação de substâncias de grande interesse biológico e ambiental, tais como os compostos fenólicos (4-aminofenol e serotonina). Neste sentido, a síntese dos MIPs foi baseada na polimerização convencional em "bulk". Cada polímero foi sintetizado a partir do ácido metacrílico (monômero funcional), etileno glicoldimetilacrilato (reagente de ligação cruzada), 2¿2-azo-bis-isobutironitrila (iniciador radicalar), em presença de Fe(III)protoporfrina(IX) (hemina) como centro catalítico, o qual é responsável pela mimetização do sítio ativo da peroxidase, criando portanto, um polímero com impressão molecular cataliticamente ativo para o reconhecimento do 4-aminofenol e serotonina (moléculas molde). Além disso, a fim de avaliar a seletividade do material, foram preparados, paralelamente, polímeros sem a impressão molecular (NIP Non Imprinted Polymers) e também na ausência de hemina. Os MIPs foram caracterizados pelas técnicas de espectroscopia no infravermelho, área superficial específica, volume específico dos poros, análise termogravimétrica, microscopia eletrônica de varredura. Parâmetros cinéticos, incluindo valores de velocidade máxima, Vmax e constante aparente de Michaelis¿Menten, Km foram obtidas pelo gráfico de Lineweaver-Burk. Para aplicação analítica, em amostras de água e soro sanguíneo, sistemas amperométricos foram otimizados através de análise multivariada
Abstract: The present work describes the applications of biomimetic nanoreactor to the based peroxidase in molecularly imprinted polymers (MIP) as a promising tool for determination of substances of high biological and environmental interest, such as phenolic compounds (4-aminophenol and serotonin). In this sense, the synthesis of MIPs was based on the conventional polymerization in bulk. Each polymer was synthesized from methacrylic acid (functional monomer), ethylene glycol dimethacrylate (cross-linking reagent), 2,2'-azobis-isobutyronitrile (initiator), in the presence of Fe(III)protoporphyrin(IX) (hemin) as a catalytic center, which is responsible for the mimic of the active site of peroxidase, creating therefore, a molecularly imprinted polymer active catalytically for the recognition of the 4-aminophenol and serotonin (template molecules). Furthermore, in order to evaluate the selectivity of the material, were prepared, parallel, polymers without the molecular impression (NIP - Non imprinted polymers) and also in the hemin absence. The MIPs were characterized by the techniques of infrared spectroscopy, specific surface area, specific pore volume, thermogravimetric analysis, scanning electron microscopy. Kinetic parameters, including values for maximum rate, Vmax and Michaelis-Menten apparent constant, Km were obtained from Lineweaver-Burk plots. For analytical application, in samples of water and blood serum, amperometric systems were optimized through multivariate analysis
Doutorado
Quimica Analitica
Doutor em Ciências
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COSTA, Alessandra Carolina da. "Síntese e estudo de um polímero de impressão molecular (Molecularly Imprinted Polymer, MIP) usando bisfenol A como molécula molde para aplicação em extração em fase sólida (Molecularly Imprinted Solid Phase Extraction, MISPE)." reponame:Repositório Institucional da UNIFEI, 2017. http://repositorio.unifei.edu.br/xmlui/handle/123456789/1031.
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Para otimizar o processo de síntese do polímero de impressão molecular (MIP) foi realizado um planejamento de triagem estatística Box-Behnken codificados em três níveis, sendo, baixo, médio e alto (-1, 0 e +1) e três fatores, sendo, tipo de solvente, tempo de reação e velocidade de agitação. A técnica cromatográfica utilizada foi a Cromatografia Líquida de Alta Eficiência (CLAE), com detector por Fluorescência (FLD), validada e otimizada de acordo com as figuras de mérito (seletividade, sensibilidade, linearidade, precisão, exatidão, robustez, limite de detecção e limite de quantificação). Para a síntese do MIP, em uma ampola de síntese adicionou-se o BFA como molécula-molde (do inglês Template), ácido metacrílico (MAA) como monômero funcional, o solvente acetonitrila, clorofórmio ou tolueno, etilenoglicol dimetacrilato (EGDMA) como agente de ligação cruzada e o 4,4’-azo-(ácido-4-cianovalérico) (AACV) como iniciador radicalar. Também foi realizado a síntese de um polímero não impresso (NIP) ausente de molécula molde BFA. Após testes prévios da adsorção e de seletividade dos MIPs empacotados em cartucho de extração em fase sólida foram selecionados os melhores materiais poliméricos para estudos posteriores. O MIP sintetizado apresentou aparência de polímero amorfo, aspectos esbranquiçados e uma estrutura aparentemente rígida. A recuperação do MIP ficou compreendida na faixa de 77,20 – 87,54% com um coeficiente de variação (CV) inferior a 10%. A recuperação do polímero não impresso foi inferior à do MIP. A quantidade remanescente de molécula-molde presente no MIP foi inferior a 1% indicando que a técnica por extração Soxhlet utilizada para a remoção da molécula-molde foi eficiente. A seletividade do MIP e NIP otimizado foi comparada com o composto fenol que se mostrou seletivo para o MIP e NIP com uma recuperação de 48,20% (MIP) e 48,25% (NIP) com CV inferior a 45%. Os materiais poliméricos sintetizados foram caracterizados por espectroscopia de absorção molecular na região do infravermelho por transformada de Fourier (FTIR), microscopia eletrônica por varredura (MEV), analisador de tamanho de partículas, analisador de área superficial e porosidade por adsorção/dessorção com nitrogênio pelo método B.E.T, análise termogravimétrica (TGA) e calorimetria exploratória diferencial (DSC).
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Gonzato, Carlo. "Chemical nanosensors based on molecularly imprinted polymer nanocomposites synthesized by controlled radical polymerization." Compiègne, 2012. http://www.theses.fr/2012COMP2035.
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Les polymères à empreintes moléculaires (MIP, pour molecularly imprinted polymers en anglais) sont des récepteurs synthétiques, parfois appelés "anticorps en plastique", capables de reconnaitre et fixer spécifiquement une molécule cible. L’impression moléculaire s’est imposée, durant les trente dernières années, comme une technique pour la synthèse des structures réticulées possédant une remarquable affinité et sélectivité vis-à-vis d’une espèce chimique utilisée comme molécule empreinte dans un procédé de moulage au niveau moléculaire. La grande variété de matériaux et de formats accessibles à cette technique lui ont permis de trouver un grand nombre d’applications, telles que la séparation, les capteurs, la catalyse, le ré-largage contrôlé de médicaments. Depuis leur apparition, la plupart des MIPs a été synthétisée par polymérisation radicalaire libre (FRP) des monomères vinyliques. Cette méthode de polymérisation représente un excellent choix en termes de simplicité de mise en place, tolérance par rapport aux solvants et aux différents groupements fonctionnels des ingrédients. Cependant, plusieurs désavantages liés à cette technique limitent la possibilité d’obtenir un contrôle adéquate vis-à-vis de certaines caractéristiques fondamentales pour des applications en nano-technologies. L’introduction des techniques de polymérisation radicalaire contrôlée/vivante (CRP) a donc représentée une avancée importante et a permis de dépasser certains limites associés aux MIPs synthétisés par FRP. Dans ce contexte, ce travail de thèse a étudié les avantages provenant de l’utilisation d'une méthode CRP, le RAFT, pour la synthèse des MIP. Ce travail a été mené en se focalisant sur les caractéristiques principales des CRPs : le caractère vivant et, en même temps, contrôlé. Dans un premier temps, nous avons utilisé l’aspect vivant de la polymérisation pour la synthèse des nanocomposites MIP, possédant des propriétés superparamagnétiques. Celle-ci a été effectuée par polymérisation de couches p(EGDMA-co-MAA) par RAFT amorcée à la surface des particules de Fe3O4 préfonctionnalisées avec des groupements amine. Le greffage de ces couches a été obtenu en employant des ultrasons comme source d'agitation, et en testant différents solvants pour en apprécier l’influence sur la structure et la morphologie des composites résultants. Nous avons démontré que le greffage se produit d’une façon homogène, et que grâce au caractère vivant de la polymérisation RAFT, les composites peuvent être fonctionnalisés davantage, par exemple par des chaines p(EGMP), pour ajuster leur propriétés de surface. Dans un deuxième temps, nous nous sommes consacrés à une étude comparative visant la mise en évidence des avantages de la RAFT par rapport à la FRP en termes de performances des MIP acryliques et méthacryliques. Pour mieux apprécier les différences induites par la méthode de polymérisation, le dégrée de réticulation et donc la flexibilité des réseaux ont été variés de façon systématique. Cette stratégie a permis de bien apprécier les différences induites par chaque technique de polymérisation. Les résultats ont démontré que la RAFT permet de synthétiser des MIPs ayant une meilleure affinité pour leur molécule cible, et que cette amélioration est due à une distribution plus homogène des points de réticulation au sein du réseau. Finalement, nous avons appliqué la RAFT pour la synthèse de nanocapteurs individuels basés sur des composite MIP intégrant des nanoparticules d'or, et utilisant la spectroscopie Raman exaltée (SERS) pour la détectionMolecularly imprinted polymers (MIPs) are synthetic receptors, also known as antibody mimics, that can specifically bind target molecules. Molecular imprinting has emerged, over the last 30 years; it is an extremely versatile strategy for synthesizing networks possessing high affinity and selectivity for a chemical species, used as a molecular template during their synthesis. The wide variety of materials and formats that are accessible through this strategy has resulted in a broad spectrum of applications for such MIPs, ranging from separation to sensing, catalysis, drug delivery, etc. Since the beginning, the great majority of the imprinted networks has been synthesized by assembling vinyl monomers via free-radical polymerization (FRP). This polymerization method represents a convenient choice for synthesizing MIPs, due to its easy setup, versatility, tolerance with respect to many solvents and functional groups. However, some drawbacks greatly affect the possibility of achieving of suitable degree of control over some “polymeric” parameters which become important for specific applications. The introduction of controlled/”living” radical polymerization (CRP) techniques has then represented an opportunity for MIPs to reduce, and in some cases even to overcome, some of their limits arising from FRP. In this respect, this Ph. D. Thesis has studied how the use of RAFT polymerization, one of the most applied CRPs, can be advantageously used to syntheze MIPs. This has been done by focusing on the main characteristics of CRPs: their living and controlled nature. The living nature has been exploited during the first part of this work, which involved the synthesis of superparamagnetic molecularly imprinted nanocomposites via surface-initiated RAFT polymerization of p(EGDMA-co-MAA) on amino-modified Fe3O4 nanoparticles. The polymer grafting has been performed using an unusual stirring technique (i. E. Ultrasonication) during the polymerization step, and by testing different polymerization solvents for evaluating their effect on the composite structure. It has been observed that the grafting resulted in homogeneous polymer layers, the thickness of which could be controlled by adjusting the RAFT/radical source ratio. Moreover, the living nature of RAFT fragments has been exploited for post-functionalizing the surface of a composite particle with p(EGMP) brushes, thus demonstrating the potential of fine-tuning the particle surface properties through the living chain ends. In the second part of the thesis, an in-depth study has been performed on the effects induced by the use of controlled (RAFT) polymerization conditions on the binding behaviour and structural parameters of bulk acrylic and methacrylic MIPs and the corresponding non-imprinted polymers, synthesized by RAFT and FRP with varying cross-linking degree. This strategy actually provided scaffolds with progressively increased degree of flexibility (especially in the case of acrylics) which allowed visualize the enhancement of binding and structural differences arising from the polymerization technique. As a result, it has been observed that the use of controlled (RAFT) conditions induced, on the imprinted networks, an increased template affinity over equivalent FRPs, and it has been demonstrated that this improved affinity can be related to more homogeneous distributions of the cross-linking points achieved during RAFT polymerization. The third part presents preliminary results toward the synthesis by RAFT of individual multi-composite MIP nanosensors using enhanced Raman spectroscopy (SERS) for detection
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Wagner, Sabine [Verfasser], Ulrich [Gutachter] Panne, Kannan [Gutachter] Balasubramanian, and Annabelle [Gutachter] Bertin. "Sensory molecularly imprinted polymer (MIP) coatings for nanoparticle- and fiber optic-based assays / Sabine Wagner ; Gutachter: Ulrich Panne, Kannan Balasubramanian, Annabelle Bertin." Berlin : Humboldt-Universität zu Berlin, 2019. http://d-nb.info/1188714554/34.
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Krstulja, Aleksandra. "Development of molecularly imprinted polymers for the recognition of urinary nucleoside cancer biomarkers." Thesis, Orléans, 2015. http://www.theses.fr/2015ORLE2009.
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Ce rapport de thèse présente l’étude de la technologie des empreintes moléculaires pour le développement de polymères spécifiques et sélectifs envers des biomarqueurs urinaires nucléosidiques du cancer colorectal chez l’Homme. L’objectif principal était de développer des polymères à empreintes moléculaires compatibles aux milieux aqueux en utilisant la technique du « dummy template », l’approche non-covalente and la polymérisation radicalaire en masse. Nous nous sommes concentrés principalement sur la qualité des polymères à partir de leur formulation, c’est-à-dire la spécificité et la sélectivité. Cela a été mené de façon empirique d’abord par la production de poudres issues de polymères monolithiques. Ainsi, pour atteindre les objectifs fixés, nous avons exploré le choix de la molécule « template ». Une étude de modèle est présentée au chapitre 3, en utilisant trois nucléosides 2’,3’,5’-peracétylés comme molécule empreinte dans une approche « dummy template ». Ensuite, en s’appuyant sur la connaissance apportée par le chapitre 3, nous avons développé des polymères à empreintes moléculaires (MIPs) sélectifs de la pseudouridine et de la N7-méthylguanosine dans les chapitres 4 et 5, respectivement, en utilisant la 2’,3’,5’-tri-O-acétylpseudouridine et la 2’,3’,5’-tri-O-acétylguanosine comme templates. L’étude de la rétention des nucléosides recherchés et de leurs analogues structuraux menée par chromatographie en phase liquide et par analyse frontale a permis de déterminer la capacité des différents polymères et de connaître leur comportement dans de l’urine synthétique. Finalement, pour évaluer la possible application de ces polymères dans un échantillon réel, l’urine humaine, la technique de l’extraction sur phase solide à empreintes moléculaires ou MISPE a été développée. Ainsi, une purification sélective des biomarqueurs cibles, tels que la pseudouridine et la N7-méthylguanosine, dans des échantillons d’urines a pu être démontréeThis thesis report presents the exploration of molecularly imprinted polymer (MIP) technology for developing of a sensitive and selective polymers used in urinary nucleoside biomarker recognition. The main goal was to develop water compatible MIPs prepared by a “dummy template” imprinting technology, using a non-covalent approach and radical-polymerization in bulk. We were focusing mostly on the polymer quality in the formulation (rigidity, stability and repeatability). This was chosen empirically first by production of powders from monolithic MIP. Thus, to accomplish the stated goals, we have explored the choice of the template molecule. A model study presented by Chapter 3, using three 2’3’5’-tri-Operacylateduridine nucleosides as templates in a “dummy” template approach was first developed. Then, applying the knowledge of the type of template choice, we developed a selective MIP for recognition of pseudouridine and N7-methylguanosine in the studies presented in Chapter 4 and Chapter 5 respectively. By using 2’3’5’-tri-O-acetylpseudouridine and 2’3’5’-tri-O-acetylguanosine as templates. Chromatographic methods like HPLC retention and frontal analysis were used in the interest of determining the binding capacity of synthesized polymers, and the behavior in synthetic urine. Finally, to evaluate the possible application of these polymers in urine, molecularly imprinted solid phase extraction (MISPE) was developed. Selective purification of urine samples containing pseudouridine and N7-methylguanosine obtained in the end
Book chapters on the topic "MIP [Moleculary imprinted polymer]"
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Cieplak, Maciej, and Wlodzimierz Kutner. "CHAPTER 9. Protein Determination Using Molecularly Imprinted Polymer (MIP) Chemosensors." In Polymer Chemistry Series, 282–329. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010474-00282.
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Cyago, Allan, and Rigoberto Advincula. "Surface Plasmon Resonance Spectroscopy and Molecularly Imprinted Polymer (MIP) Sensors." In Handbook of Spectroscopy, 1229–58. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527654703.ch33.
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Furtado, Ana I., Raquel Viveiros, and Teresa Casimiro. "MIP Synthesis and Processing Using Supercritical Fluids." In Molecularly Imprinted Polymers, 19–42. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1629-1_3.
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Feng, Jing, and Zhaosheng Liu. "MIP as Drug Delivery Systems of Anticancer Agents." In Molecularly Imprinted Polymers as Advanced Drug Delivery Systems, 133–52. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0227-6_7.
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Zhao, Long, and Zhaosheng Liu. "MIP as Drug Delivery Systems of Ophthalmic Drugs." In Molecularly Imprinted Polymers as Advanced Drug Delivery Systems, 153–78. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0227-6_8.
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Wei, Zehui, Lina Mu, and Zhaosheng Liu. "MIP as Drug Delivery Systems for Dermal Delivery." In Molecularly Imprinted Polymers as Advanced Drug Delivery Systems, 111–31. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0227-6_6.
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Ma, Li, and Zhaosheng Liu. "MIP as Drug Delivery Systems for Special Application." In Molecularly Imprinted Polymers as Advanced Drug Delivery Systems, 179–200. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-0227-6_9.
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Alexander, Cameron, and Nicole Kirsch. "New methodologies in the preparation of imprinted polymers." In Polymer Chemistry. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780198503095.003.0013.
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Molecular imprinting is a rapidly emerging method for the creation of recognition sites in synthetic polymers, and the resultant materials offer considerable promise as selective adsorbents in a number of applications. The technique exploits the principle of using elements of a target molecule to create its own recognition site. This is achieved by the formation of a highly cross-linked polymeric matrix around a template, which can be the target molecule itself or a close structural analogue. The key to this procedure is to ensure that, during the polymerization, functional groups of the template molecule are fully engaged in interactions with ‘complementary functionality’ of polymer-forming components. These interactions are then ‘locked in’ by the incorporation of the whole assembly into the polymer structure. Subsequent removal of the template reveals the newly created binding sites containing functional groups in the precise stereochemical arrangement to ensure recognition of the target in a highly selective manner (Scheme 1). The first reports of molecular imprinting in organic polymers involved the templating of protected sugars, in the form of esters with a polymerizable boronic acid (however, see Ref. 18 for an earlier example of the imprinting concept) into a cross-linked polymer ‘scaffold’, and variations of the basic technique have now been adopted by many research groups around the world. In general, molecularly imprinted polymers (MIPs) are prepared by thermal or photochemical free-radical routes, employing acrylic or vinylic monomers in a solvent chosen to ensure that the final matrix is microporous. The numbers and types of molecules which have now been imprinted is very large, but a key factor in the preparation of MIP materials with the desired recognition properties is still the chemical nature of the link between the template and the polymer backbone. Consequently, strategies by which the template can be securely fixed in space as the growing matrix forms around it, yet be readily removed to generate the recognition site after polymer synthesis is complete, are of particular interest.
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Türkoğlu, Emir Alper. "Molecularly-Imprinted Nanomaterial-Based Surface Plasmon Resonance Biosensors in Molecular Diagnosis." In Applications of Nanomaterials in Agriculture, Food Science, and Medicine, 1–28. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-5563-7.ch001.
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Molecular diagnosis is based on target detection associated with many diseases and abnormalities. Common analytical techniques have disadvantages in the diagnosis. In contrast, biosensors are performed quick and selective detection of various markers. Surface plasmon resonance (SPR) biosensor allows qualitative and quantitative measurements without labelling and frequently used in medical applications. Recent developments in various emerging disciplines have allowed the design of novel bioreceptors for SPR biosensors. In recent years, the use of bioreceptors combined with nanotechnology and molecular imprinting technologies has increased in SPR instruments. Molecularly imprinted polymers (MIPs) are synthetic polymeric materials and provide specific and selective recognition sites for the target of interest. MIPs as bioreceptor show unique features compared to natural receptors. Therefore, MIP-based bioreceptors in SPR sensors have gained more attention recently. This chapter primarily focuses on the status and applications of nano-MIP-based SPR biosensors for the molecular diagnosis.
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Dembaremba, Tendai O., Adeniyi S. Ogunlaja, and Zenixole R. Tshentu. "Coordination Polymers and Polymer Nanofibers for Effective Adsorptive Desulfurization." In Nanocomposites for the Desulfurization of Fuels, 168–234. IGI Global, 2020. http://dx.doi.org/10.4018/978-1-7998-2146-5.ch006.
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Desulfurization of fuel oils is an essential process employed in petroleum refineries to reduce the sulfur content to levels mandated for environmental protection. Hydrodesulfurization (HDS), which is currently being employed, is limited in treating refractory organosulfur compounds and only reduces the sulfur content in fuels to a range of 200-500 ppmS. In this chapter, several scientific and technological advances reported in the literature for the desulfurization of fuels are reviewed and discussed. Amongst these techniques, oxidative desulfurization (ODS) and adsorptive desulfurization (ADS) are proposed as additional steps to complement HDS in meeting the mandated ultra-low sulfur levels (10 ppmS). In the ODS technique, refractory organosulfur compounds are oxidized to organosulfones, followed by solvent extraction or adsorption of the organosulfones. The chemistry involved in the development and fabrication of sulfur/sulfone responsive adsorbents is also discussed. The use of molecular imprinted polymers (MIPs) and coordination polymers (CPs) for the selective adsorption of organosulfone compounds (in ODS) and/or organosulfur (in ADS) offers various properties such as imprinting effect, hydrogen bonding, π-π interactions, van der Waals forces, π-complexation, and electrostatic interactions. CPs, in particular metal organic frameworks (MOFs), have been reported to possess suitable features to overcome most of these challenges associated with adsorptive ultra-deep desulfurization when design strategies to achieve good selectivity are strictly followed. Matching the sizes of the cavities to the critical dimensions of the sulfur containing compounds (SCCs), using suitable metal centres which allow for coordinative interaction with the SCCs and using linkers with suitable functionality as to enhance specific interaction (dispersion forces) with the SCCs were considered to be pivotal features to prioritize. The prospects for the use of MIPs and CPs for future industrial applications in desulfurization are envisaged.
Conference papers on the topic "MIP [Moleculary imprinted polymer]"
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Nurhamidah, Nurhamidah, Popo Marinda, and Erri Koryanti. "PEMBUATAN MOLECULARLY IMPRINTED POLYMER (MIP) MELAMIN MENGGUNAKAN METODE COOLING-HEATING." In SEMINAR NASIONAL FISIKA 2017 UNJ. Pendidikan Fisika dan Fisika FMIPA UNJ, 2017. http://dx.doi.org/10.21009/03.snf2017.02.mps.08.
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Vitale, U., A. Rechichi, M. D’Alonzo, C. Cristallini, N. Barbani, G. Ciardelli, and P. Giusti. "Selective Peptide Recognition With Molecularly Imprinted Polymers in Designing New Biomedical Devices." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95587.
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Molecular imprinting is a technique for the synthesis of polymers capable to bind selectively specific molecules. The imprinting of large proteins, like cell adhesion proteins or cell receptors, can lead to important and innovative biomedical applications. However such molecules show such important conformational changes in the polymerisation environment that the recognition sites are poorly specific. The “epitope approach” can overcome this limit by adopting, as template, a stable short peptide sequence representative of an accessible fragment of a larger protein. The resulting imprinted polymer can recognize both the template and the whole molecule thanks to the specific cavities for the epitope. In this work two molecularly imprinted polymer formulations (macroporous monolith and nanospheres) were obtained with the protected peptides Z-Thr-Ala-Ala-OMe, as template, and Z-Thr-Ile-Leu-OMe, as analogue for the selectivity evaluation, the methacrylic acid, as functional monomer, the trimethylolpropane trimethacrylate and pentaerythritol triacrylate, as cross-linkers. Polymers were synthesized by precipitation polymerisation in acetonitrile at 60 °C, thermally initiated with azobisisobutyronitrile. All polymers were characterized by the standard techniques SEM, FT-IR, and TGA. The supernatants from the polymerisation and the rebinding solutions were analysed by HPLC. The higher cross-linked polymers retained about the 70% of the template, against about the 20% for the lower ones. The extracted template amount and the rebinding capacity decreased with the cross-linking degree, while the selectivity showed the opposite behaviour. The pentaerythritol triacrylate cross-linked polymers showed the best recognition (MIP 2−, α = 1.71) and selectivity (MIP 2+, α′ = 5.58) capabilities.
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Holthoff, Ellen L., Lily Li, Tobias Hiller, and Kimberly L. Turner. "A molecularly imprinted polymer (MIP)-coated microbeam MEMS sensor for chemical detection." In SPIE Defense + Security, edited by Augustus W. Fountain. SPIE, 2015. http://dx.doi.org/10.1117/12.2179694.
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García-Garibay, M., I. Méndez-Palacios, A. López-Luna, E. Bárzana, and J. Jiménez-Guzmán. "Development of a Molecularly Imprinted Polymer (MIP) for the Recovery of Lactoferrin." In 13th World Congress of Food Science & Technology. Les Ulis, France: EDP Sciences, 2006. http://dx.doi.org/10.1051/iufost:20060639.
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Kia, Solmaz. "A new Voltametric sensor, based on molecularly imprinted polymer (MIP) for vitamin D3 Detection." In 2019 International Conference on Biomedical Innovations and Applications (BIA). IEEE, 2019. http://dx.doi.org/10.1109/bia48344.2019.8967459.
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Aouled, N. Omar, H. Hallil, B. Plano, D. Rebiere, C. Dejous, R. Delepee, and L. Agrofoglio. "Love wave sensor based on thin film molecularly imprinted polymer : MIP layer morphology and nucleosides analogs detection." In 2013 IEEE Sensors. IEEE, 2013. http://dx.doi.org/10.1109/icsens.2013.6688280.
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Naskar, Hemanta, Sheikh Saharuk Ali, A. H. M. Toufique Ahmed, Debangana Das, Shreya Nag, Bipan Tudu, and Rajib Bandyopadhyay. "Detection of Curcumin using a Simple and Sensitive Molecularly Imprinted Polymer (MIP) Embedded Graphite Electrode Based Electrochemical Sensor." In 2020 International Conference on Emerging Frontiers in Electrical and Electronic Technologies (ICEFEET). IEEE, 2020. http://dx.doi.org/10.1109/icefeet49149.2020.9186985.
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Sianita, Maria Monica, Ni Nyoman Tri Puspaningsih, Miratul Khazanah, and Gaden Supriyanto. "Comparison of the method used for extraction chloramphenicol from its Molecularly Imprinted Polymer (MIP) using chloroform as porogen." In Proceedings of the National Seminar on Chemistry 2019 (SNK-19). Paris, France: Atlantis Press, 2019. http://dx.doi.org/10.2991/snk-19.2019.5.
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Hawari, H. F., N. M. Samsudin, A. Y. Shakaff, A. Zakaria, S. A. Ghani, M. N. Ahmad, Pusat Pengajian Jejawi II, Taman Muhibbah, Y. Wahab, and U. Hashim. "5.2.1 Development of Highly Selective Interdigitated Electrode (IDE) Sensor Array using Molecular Imprinted Polymer (MIP) for Detection of Mango Fruit Ripeness." In 14th International Meeting on Chemical Sensors - IMCS 2012. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2012. http://dx.doi.org/10.5162/imcs2012/5.2.1.
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Ciardelli, G., F. M. Montevecchi, P. Giusti, D. Silvestri, I. Morelli, C. Cristallini, and G. Vozzi. "Molecular Imprinted Nanostructures in Biomedical Applications." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95669.
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Molecular imprinting is an emerging technology that allows to introduce nanostructured cavities into a polymer. In preparing molecular imprinted polymers (MIPs), the functional monomer(s) is first prearranged around the template molecule by specific interactions; the polymerisation is then carried out with a high percentage of cross-linking agent (which “freezes” the macromolecular network). Molecular mechanics and dynamics can be used to gain indications on the best monomers to be used in order to maximize interactions with the template. Once the polymerization reaction has been completed, the template is removed from the rigid three-dimensional network, leaving free recognition cavities available for the successive selective rebinding of the template itself. Precipitation polymerisation in dilute solutions involves the spontaneous formation of submicron scale polymer particles, which result suitable for recognition-rebinding application. Therapeutic applications: The recognition mechanism by MIPs relies mainly on the establishment of reversible hydrogen bonding interactions. It is clear that the efficiency of this mechanism is endangered in aqueous environments. MIPs working in water solutions are clearly of great interest in the medical and food industry and in sensor applications. We recently overcame these difficulties by the realisation of a system where cross-linked MI methylmethacrylate-methacrylic acid nanospheres where loaded on the surface or inside the matrix of porous membranes created by phase inversion. E.g. membranes were modified by adding cholesterol imprinted nanoparticles. Rebinding performances of nanoparticles modified membranes in buffer solution were tested showing a specific recognition of 14.09 mg of cholesterol/g of system (membrane and nanoparticles), indicating maintained binding capacity of supported particles as well. Tissue engineering: The engineering of functionalised polymeric structures for the study of cell activity is essential to the development of biological substitutes containing vital cells capable of regenerating or enhancing tissue function. Cells are organised within a complex matrix consisting of high molecular weight protein and polysaccharides known as the Extracellular Matrix (ECM). Two approaches are described to explore the possibility to provide scaffolds with specific and selective recognition of peptide sequences or proteins involved in cell adhesion mechanisms: one approach consists in the modification of porous structures with nanoparticles imprinted with aminoacid sequences (epitopes) of ECM proteins or transmembrane integrins, while the other consists in the combination of Soft Litography and Molecular Imprinting technologies (SOFT-MI). This technology allows to create imprinting nanocavities selective towards ECM proteins in microfabricated scaffolds, and in particular it permits to realise patterns with a well defined microscale geometry in polymethylmethacrylate (PMMA) scaffolds providing them with cell adhesion properties that were missing in the non-imprinted scaffold.
Reports on the topic "MIP [Moleculary imprinted polymer]"
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Holthoff, Ellen L., Lily Li, Tobias Hiller, and Kimberly L. Turner. A Molecularly Imprinted Polymer (MIP)-Coated Microbeam MEMS Sensor for Chemical Detection. Fort Belvoir, VA: Defense Technical Information Center, September 2015. http://dx.doi.org/10.21236/ada622335.
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Harvey, Scott D. Ultraselective Sorbents. Task 2: Molecularly Imprinted Polymers (MIPs)/Stabilized Antibody Fragments (STABs). Final Report FY 2004. Office of Scientific and Technical Information (OSTI), September 2004. http://dx.doi.org/10.2172/15016482.
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Holthoff, Ellen, and Dimitra Stratis-Cullum. A Nanosensor for Explosives Detection Based on Molecularly Imprinted Polymers (MIPs) and Surfaced-enhanced Raman Scattering (SERS). Fort Belvoir, VA: Defense Technical Information Center, March 2010. http://dx.doi.org/10.21236/ada516676.
Full text
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Harvey, Scott D. Ultraselective Sorbents. Task 2: Molecularly Imprinted Polymers (MIPs)/Stabilized Antibody Fragments (STABs). Final Report -- Fiscal Year (FY) 2005. Office of Scientific and Technical Information (OSTI), September 2005. http://dx.doi.org/10.2172/860003.
Full text