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Journal articles on the topic 'Mixed bacterial population'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Venkateswaran, Kasthuri, Hiroki Tanaka, Shyoko Komukai, Haruhisa Toki, Tokuro Iwabuchi, and Shigetoh Miyachi. "ECODYNAMICS OF OIL-DEGRADING BACTERIA AND SIGNIFICANCE OF MARINE MIXED POPULATIONS IN THE DEGRADATION OF PETROLEUM COMPOUNDS." International Oil Spill Conference Proceedings 1993, no. 1 (1993): 427–34. http://dx.doi.org/10.7901/2169-3358-1993-1-427.

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ABSTRACT Ecological studies, screening of hydrocarbon-degrading bacteria, and studies of the potentials of various single and mixed bacterial populations in the utilization of petroleum compounds were carried out to understand the microbial hydrocarbon degradation process in marine ecosystems. Populations of hydrocarbon utilizers were larger in coastal regions than in pelagic environments. Ecological observations indicated that oil-degrading bacteria were ubiquitously distributed in both temperate and tropical environments, irrespective of oil-polluted and unpolluted ecosystems. Bacteria were

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Pernthaler, Jakob, Thomas Posch, Karel S̆imek, et al. "Predator-Specific Enrichment of Actinobacteria from a Cosmopolitan Freshwater Clade in Mixed Continuous Culture." Applied and Environmental Microbiology 67, no. 5 (2001): 2145–55. http://dx.doi.org/10.1128/aem.67.5.2145-2155.2001.

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ABSTRACT We investigated whether individual populations of freshwater bacteria in mixed experimental communities may exhibit specific responses to the presence of different bacterivorous protists. In two successive experiments, a two-stage continuous cultivation system was inoculated with nonaxenic batch cultures of the cryptophyteCryptomonas sp. Algal exudates provided the sole source of organic carbon for growth of the accompanying microflora. The dynamics of several 16S rRNA-defined bacterial populations were followed in the experimental communities. Although the composition and stability o

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Galera-Laporta, L., and J. Garcia-Ojalvo. "Antithetic population response to antibiotics in a polybacterial community." Science Advances 6, no. 10 (2020): eaaz5108. http://dx.doi.org/10.1126/sciadv.aaz5108.

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Much is known about the effects of antibiotics on isolated bacterial species, but their influence on polybacterial communities is less understood. Here, we study the joint response of a mixed community of nonresistant Bacillus subtilis and Escherichia coli bacteria to moderate concentrations of the β-lactam antibiotic ampicillin. We show that when the two organisms coexist, their population response to the antibiotic is opposite to that in isolation: Whereas in monoculture B. subtilis is tolerant and E. coli is sensitive to ampicillin, in coculture it is E. coli who can proliferate in the pres

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Shiomi, Yoshitaka, Masaya Nishiyama, Tomoko Onizuka, and Takuya Marumoto. "Comparison of Bacterial Community Structures in the Rhizoplane of Tomato Plants Grown in Soils Suppressive and Conducive towards Bacterial Wilt." Applied and Environmental Microbiology 65, no. 9 (1999): 3996–4001. http://dx.doi.org/10.1128/aem.65.9.3996-4001.1999.

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ABSTRACT It has been reported that the growth of Ralstonia solanacearum is suppressed at the rhizoplane of tomato plants and that tomato bacterial wilt is suppressed in plants grown in a soil (Mutsumi) in Japan. To evaluate the biological factors contributing to the suppressiveness of the soil in three treated Mutsumi soils (chloroform fumigated soil; autoclaved soil mixed with intact Mutsumi soil; and autoclaved soil mixed with intact, wilt-conducive Yamadai soil) infested with R. solanacearum, we bioassayed soil samples for tomato bacterial wilt. Chloroform fumigation increased the extent of

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Plumb, Jason J., Joanne Bell, and David C. Stuckey. "Microbial Populations Associated with Treatment of an Industrial Dye Effluent in an Anaerobic Baffled Reactor." Applied and Environmental Microbiology 67, no. 7 (2001): 3226–35. http://dx.doi.org/10.1128/aem.67.7.3226-3235.2001.

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ABSTRACT Fluorescent in situ hybridization (FISH) using 16S and 23S rRNA-targeted probes together with construction of an archaeal 16S ribosomal DNA (rDNA) clone library was used to characterize the microbial populations of an anaerobic baffled reactor successfully treating industrial dye waste. Wastewater produced during the manufacture of food dyes containing several different azo and other dye compounds was decolorized and degraded under sulfidogenic and methanogenic conditions. Use of molecular methods to describe microbial populations showed that a diverse group of Bacteria andArchaea was

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Bashan, Yoav, and Luz E. de-Bashan. "Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense." Applied and Environmental Microbiology 68, no. 6 (2002): 2637–43. http://dx.doi.org/10.1128/aem.68.6.2637-2643.2002.

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ABSTRACT Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato, and the plant growth-promoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. The population dynamics in the rhizosphere and foliage, the development of bacterial speck disease, and their effects on plant growth were monitored. When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. tomato (107 versus 105 CFU/g [dr

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Coallier, Josée, Michèle Prévost, Annie Rompré, and Daniel Duchesne. "The optimization and application of two direct viable count methods for bacteria in distributed drinking water." Canadian Journal of Microbiology 40, no. 10 (1994): 830–36. http://dx.doi.org/10.1139/m94-132.

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The optimal incubation conditions for the direct viable count method with nalidixic acid were determined. They do not differ from those proposed in the literature for a laboratory strain and a mixed bacterial population isolated from drinking water. The direct viable count method with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was performed under in situ conditions. The bacteria were incubated with CTC at a concentration of 1 mM for 4–6 h at the temperature of the water in the pipes and without the addition of an exogenous substrate. The results obtained for a laboratory strain using the t

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8

Lee, Anna J., Shangying Wang, Hannah R. Meredith, Bihan Zhuang, Zhuojun Dai та Lingchong You. "Robust, linear correlations between growth rates and β-lactam–mediated lysis rates". Proceedings of the National Academy of Sciences 115, № 16 (2018): 4069–74. http://dx.doi.org/10.1073/pnas.1719504115.

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It is widely acknowledged that faster-growing bacteria are killed faster by β-lactam antibiotics. This notion serves as the foundation for the concept of bacterial persistence: dormant bacterial cells that do not grow are phenotypically tolerant against β-lactam treatment. Such correlation has often been invoked in the mathematical modeling of bacterial responses to antibiotics. Due to the lack of thorough quantification, however, it is unclear whether and to what extent the bacterial growth rate can predict the lysis rate upon β-lactam treatment under diverse conditions. Enabled by experiment

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Sulistinah, Nunik, S. Antonius, and Maman Rahmansyah. "PENGARUH RESIDU PESTISIDA TERHADAP POLA POPULASI BAKTERI DAN FUNGI TANAH DI RUMAHKACA." Jurnal Teknologi Lingkungan 12, no. 1 (2016): 43. http://dx.doi.org/10.29122/jtl.v12i1.1261.

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Pesticide Residue was Affect Bacterial and Fungal Population in the Greenhouse Soil Condition. Bacterial and fungal inhabitants were examined through agriculture soil samples. Survival of microorganism in soil was important to evaluate the mineralize process. In the greenhouse experiment, bacterial and fungal population noticed as essential assessment in soil healing with long lasting pesticide (A-soil) after the soil treated with powdered rice straw as organic matter amendment, toxic degrading bacterial as inoculants, and the both of those mixed treatments. That residue implication also asses

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Hermanowicz, S. W., and F. L. Filho. "Disinfection and Attachment of Bacterial Cells." Water Science and Technology 26, no. 3-4 (1992): 655–64. http://dx.doi.org/10.2166/wst.1992.0446.

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Attachment of a mixed bacterial population isolated from well water was investigated using a rotating disk apparatus. The effects of two disinfectants (chlorine and monochloramine) applied to bacterial suspensions on subsequent cell attachment were examined. Both chlorine and chloramine appeared to adversely affect cell attachment.

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11

Malekpour, Seyed Amir, Parsa Pakzad, Mohammad-Hadi Foroughmand-Araabi, et al. "Modeling the probability distribution of the bacterial burst size via a game-theoretic approach." Journal of Bioinformatics and Computational Biology 16, no. 04 (2018): 1850012. http://dx.doi.org/10.1142/s0219720018500129.

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Based on previous studies, empirical distribution of the bacterial burst size varies even in a population of isogenic bacteria. Since bacteriophage progenies increase linearly with time, it is the lysis time variation that results in the bacterial burst size variations.Here, the burst size variation is computationally modeled by considering the lysis time decisions as a game. Each player in the game is a bacteriophage that has initially infected and lysed its host bacterium. Also, the payoff of each burst size strategy is the average number of bacteria that are solely infected by the bacteriop

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Petropoulos, Penny, and Kimberley A. Gilbride. "Nitrification in activated sludge batch reactors is linked to protozoan grazing of the bacterial population." Canadian Journal of Microbiology 51, no. 9 (2005): 791–99. http://dx.doi.org/10.1139/w05-069.

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Protozoa feed upon free-swimming bacteria and suspended particles inducing flocculation and increasing the turnover rate of nutrients in complex mixed communities. In this study, the effect of protozoan grazing on nitrification was examined in activated sludge in batch cultures maintained over a 14-day period. A reduction in the protozoan grazing pressure was accomplished by using either a dilution series or the protozoan inhibitor cycloheximide. As the dilutions increased, the nitrification rate showed a decline, suggesting that a reduction in protozoan or bacterial concentration may cause a

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van Doorn, Wouter G., and Yke de Witte. "Effect of Bacteria on Scape Bending in Cut Gerbera jamesonii Flowers." Journal of the American Society for Horticultural Science 119, no. 3 (1994): 568–71. http://dx.doi.org/10.21273/jashs.119.3.568.

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Including bacteria in the vase water of cut Gerbera jamesonii Bolus flowers resulted in an increase in scape curvature depending on the concentration of bacteria in the water, cultivar, and season. In the summer, a strain of Pseudomonas aeruginosa or a mixed population of bacterial species, all isolated from the vase water of cut gerbera flowers, resulted in curvature of >90° in `Liesbeth' at 108 cfu/ml and in `Mickey' at 1010 cfu/ml. In winter, the lowest bacterial concentrations that resulted in such bending were 106 and 108 cfu/ml, respectively. `Mickey' showed bending at a lower water p

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de Boer, Annette S., Kristin Kremer, Martien W. Borgdorff, Petra E. W. de Haas, Herre F. Heersma, and Dick van Soolingen. "Genetic Heterogeneity in Mycobacterium tuberculosisIsolates Reflected in IS6110 Restriction Fragment Length Polymorphism Patterns as Low-Intensity Bands." Journal of Clinical Microbiology 38, no. 12 (2000): 4478–84. http://dx.doi.org/10.1128/jcm.38.12.4478-4484.2000.

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Mycobacterium tuberculosis isolates with identical IS6110 restriction fragment length polymorphism (RFLP) patterns are considered to originate from the same ancestral strain and thus to reflect ongoing transmission. In this study, we investigated 1,277 IS6110 RFLP patterns for the presence of multiple low-intensity bands (LIBs), which may indicate infections with multipleM. tuberculosis strains. We did not find any multiple LIBs, suggesting that multiple infections are rare in the Netherlands. However, we did observe a few LIBs in 94 patterns (7.4%) and examined the nature of this phenomenon.

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15

Waite, Richard D., and Michael A. Curtis. "Pseudomonas aeruginosa PAO1 Pyocin Production Affects Population Dynamics within Mixed-Culture Biofilms." Journal of Bacteriology 191, no. 4 (2008): 1349–54. http://dx.doi.org/10.1128/jb.01458-08.

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ABSTRACT Transcriptomic and phenotypic studies showed that pyocins are produced in Pseudomonas aeruginosa PAO1 aerobic and anaerobic biofilms. Pyocin activity was found to be high in slow-growing anaerobic biofilms but transient in aerobic biofilms. Biofilm coculture of strain PAO1 and a pyocin-sensitive isolate showed that pyocin production had a significant impact on bacterial population dynamics, particularly under anaerobic conditions.

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16

Shooner, Frédéric, and Rajeshwar D. Tyagi. "Microbial ecology of simultaneous thermophilic microbial leaching and digestion of sewage sludge." Canadian Journal of Microbiology 41, no. 12 (1995): 1071–80. http://dx.doi.org/10.1139/m95-150.

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The microbial population encountered during a simultaneous thermophilic microbial leaching and digestion process at 50 °C, based on microbial sulfur oxidation, was investigated. The cell count of the sulfuric acid producer Thiobacillus thermosulfatus increased, followed by a decrease. In the absence of sulfur (control: conventional thermophilic digestion), Thiobacillus thermosulfatus population decreased under the detection limit. Acidophilic and neutrophilic heterotrophic populations increased during the leaching process, and the final acidophilic population count was higher than the neutroph

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17

García-Ruiz, R., J. Lucena, and F. X. Niell. "Do bacteria regenerate phosphorus while decomposing seston?" Marine and Freshwater Research 50, no. 5 (1999): 459. http://dx.doi.org/10.1071/mf98077.

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The C:P ratio of both seston in suspension and seston collected by sediment traps were systematically higher than the Redfield ratio of 106 : 1 (molar) and decreased significantly with depth in the mesotrophic, phosphorus-limited La Concepción reservoir. An aerobic decomposition experiment of seston at different concentrations by a mixed population of reservoir bacteria was performed in batch cultures to investigate the bacterial role in the changes of the C:P ratio. Dissolved phosphorus was taken up during the bacterial growth phase, while carbon was transformed to CO2 . Therefore, the C:P ra

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Silyn-Roberts, G., and G. Lewis. "Substrata effects on bacterial biofilm development in a subsurface flow dairy waste treatment wetland." Water Science and Technology 48, no. 8 (2003): 261–69. http://dx.doi.org/10.2166/wst.2003.0477.

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Biofilm development on two distinct rock substrata was investigated both in vitro and in a subsurface flow wastewater treatment wetland in order to determine the effect of hydrophobicity on initial bacterial adsorption, tertiary biofilm development and microbial population structure. Two commonly used wetland rock types, slag (a hydrophobic by-product of the steel smelter industry) and greywacke (a more hydrophilic sedimentary rock) were evaluated. In vitro investigations of initial microbial adsorption trends showed that the more hydrophobic slag displayed rapid bacterial adsorption rates com

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Vasse, Marie, Clara Torres-Barceló, and Michael E. Hochberg. "Phage selection for bacterial cheats leads to population decline." Proceedings of the Royal Society B: Biological Sciences 282, no. 1818 (2015): 20152207. http://dx.doi.org/10.1098/rspb.2015.2207.

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While predators and parasites are known for their effects on bacterial population biology, their impact on the dynamics of bacterial social evolution remains largely unclear. Siderophores are iron-chelating molecules that are key to the survival of certain bacterial species in iron-limited environments, but their production can be subject to cheating by non-producing genotypes. In a selection experiment conducted over approximately 20 bacterial generations and involving 140 populations of the pathogenic bacterium Pseudomonas aeruginosa PAO1, we assessed the impact of a lytic phage on competiti

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Avidov, Elana, Nadav Aharonson, and Jaacov Katan. "Involvement of Soil Microorganisms in the Accelerated Degradation of Diphenamid." Weed Science 38, no. 2 (1990): 186–93. http://dx.doi.org/10.1017/s0043174500056356.

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Degradation of diphenamid in soil with accelerated degradation and in nontreated control soil and the involvement of soil microorganisms in these processes were investigated. Soil with accelerated degradation and mixed bacterial cultures originating from the same soil degraded diphenamid and its monodemethylated metabolite (diphen M-1) much faster than the control. The bidemethylated derivative (diphen M-2) was degraded much more slowly than diphenamid or diphen M-1. The abundance of fungi capable of degrading diphenamid was similar in the soils with and without accelerated degradation. Degrad

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Avendaño-Pérez, Gaspar, and Carmen Pin. "Loss of Culturability of Salmonella enterica subsp. enterica Serovar Typhimurium upon Cell-Cell Contact with Human Fecal Bacteria." Applied and Environmental Microbiology 79, no. 10 (2013): 3257–63. http://dx.doi.org/10.1128/aem.00092-13.

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ABSTRACTLoss of culturability ofSalmonella entericasubsp.entericaserovar Typhimurium has been observed in mixed cultures with anaerobic fecal bacteria under conditions that allow local interaction between cells, such as cell contact. A reduction of a population of culturableS.Typhimurium on the order of ∼104to 105CFU/ml was observed in batch anaerobic mixed cultures with fecal samples from different human donors. Culturability was not affected either in supernatants collected at several times from fecal cultures, when separated from fecal bacteria by a membrane of 0.45-μm pore size, or when in

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Buonavoglia, Alessio, Gianvito Lanave, Michele Camero, et al. "Next-Generation Sequencing Analysis of Root Canal Microbiota Associated with a Severe Endodontic-Periodontal Lesion." Diagnostics 11, no. 8 (2021): 1461. http://dx.doi.org/10.3390/diagnostics11081461.

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A patient with an unusual endo-periodontal lesion, without coronal decay or damage, likely caused by a deep periodontal lesion with subsequent endodontic bacterial migration, required medical care. Next-generation sequencing (NGS) was used to assess the endodontic microbiota in vestibular and palatal canals after tooth extraction, evidencing a predominant population (Fusobacterium nucleatum) in one endodontic canal, and a mixed bacterial population with six major populations almost equally distributed in the other endodontic canal (F. nucleatum, Porphyromonas gingivalis, P. endodontis, Parvimo

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Du, Haixia, Yanxia Wu, and Huifang Wu. "Dissolved organic matter and bacterial population changes during the treatment of solid potato waste in a microbial fuel cell." Water Science and Technology 82, no. 10 (2020): 1982–94. http://dx.doi.org/10.2166/wst.2020.480.

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Abstract This study investigated the effect of mixed feeding of anaerobically cultured waste activated sludge (WAS) on the performance of microbial fuel cells (MFCs) in the treatment of solid potato waste. The maximum current densities of the four MFCs was estimated as 36, 5, 10 and 150 mA/m2, with the columbic efficiencies of 6.1, 0.3, 0.9 and 31.1%, respectively. Composition changes of dissolved organic matter (DOM) coupled with its interrelation with electricity generation and total and viable bacterial population at the end of the operation were investigated. The experimental results demon

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Yang, Hong, Bing Wang, Qing Zhang, Hui Cheng, and Zhu Yu. "Improvement of Fermentation Quality in the Fermented Total Mixed Ration with Oat Silage." Microorganisms 9, no. 2 (2021): 420. http://dx.doi.org/10.3390/microorganisms9020420.

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The use of the fermented total mixed ration (FTMR) is a promising approach for the preservation of homogeneous feed, but changes during fermentation and links with the bacterial community of FTMR are not fully understood. This study investigated the effects of adding oat silage (OS) to the fermented total mixed ration (FTMR) in terms of fermentation, chemical composition, and the bacterial community. The fermentation quality of FTMR with 22% OS was greatly improved, as demonstrated by decreases in the butyric acid concentration, a lower lactic acid/acetic acid ratio, a larger population of lac

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Ruelens, Philip, and J. Arjan G. M. de Visser. "Clonal Interference and Mutation Bias in Small Bacterial Populations in Droplets." Genes 12, no. 2 (2021): 223. http://dx.doi.org/10.3390/genes12020223.

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Experimental evolution studies have provided key insights into the fundamental mechanisms of evolution. One striking observation is that parallel and convergent evolution during laboratory evolution can be surprisingly common. However, these experiments are typically performed with well-mixed cultures and large effective population sizes, while pathogenic microbes typically experience strong bottlenecks during infection or drug treatment. Yet, our knowledge about adaptation in very small populations, where selection strength and mutation supplies are limited, is scant. In this study, wild-type

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Khan, Fazlurrahman, Sandra Folarin Oloketuyi, and Young-Mog Kim. "Diversity of Bacteria and Bacterial Products as Antibiofilm and Antiquorum Sensing Drugs Against Pathogenic Bacteria." Current Drug Targets 20, no. 11 (2019): 1156–79. http://dx.doi.org/10.2174/1389450120666190423161249.

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The increase in antibiotic resistance of pathogenic bacteria has led to the development of new therapeutic approaches to inhibit biofilm formation as well as interfere quorum sensing (QS) signaling systems. The QS system is a phenomenon in which pathogenic bacteria produce signaling molecules that are involved in cell to cell communication, production of virulence factors, biofilm maturation, and several other functions. In the natural environment, several non-pathogenic bacteria are present as mixed population along with pathogenic bacteria and they control the behavior of microbial community

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Song, M. K., and J. J. Kennelly. "Ruminal fermentation pattern, bacterial population and ruminal degradation of feed ingredients as influenced by ruminal ammonia concentration." Journal of Animal Science 68, no. 4 (1990): 1110–20. http://dx.doi.org/10.2527/1990.6841110x.

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Abstract The effects of ruminal ammonia concentration on bacterial numbers, fermentation pattern and degradation of feed ingredients in the rumen were examined with three nonlactating Holstein cows fitted with ruminal cannulas. Cows were fed twice daily a complete mixed diet (90% whole plant oat silage and 10% concentrate mixture) containing 11.1% CP on a DM basis. Ammonium bicarbonate (NH4HCO3) was infused continuously into the rumen at levels of 0, 95, 190 or 280 g/d. Infusion of NH4HCO3 linearly increased (P < .05) the concentrations of ruminal ammonia and blood urea N. Total VFA concent

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Karampoula, Doulgeraki, Fotiadis, Tampakaki, and Nychas. "Monitoring Biofilm Formation and Microbial Interactions that May Occur During a Salmonella Contamination Incident across the Network of a Water Bottling Plant." Microorganisms 7, no. 8 (2019): 236. http://dx.doi.org/10.3390/microorganisms7080236.

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The present study aims to monitor the ability of Salmonella to colonize and compete as a member of the mixed species biofilm within key points at a water bottling plant, in case of a contamination incident with this major foodborne pathogen. To achieve this goal, bacterial communities throughout the production line were collected and their identities were investigated by microbial counts and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). These bacterial communities alone or along with constructed Salmonella enterica serovar Typhimurium (ST) fluorescence-based bio

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Duncan, Alison B., Eike Dusi, Martina Schrallhammer, Thomas Berendonk, and Oliver Kaltz. "Population-level dynamics in experimental mixed infections: evidence for competitive exclusion among bacterial parasites ofParamecium caudatum." Oikos 127, no. 9 (2018): 1380–89. http://dx.doi.org/10.1111/oik.05280.

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Hudcova, Tereza, Martin Halecky, Evguenii Kozliak, Marie Stiborova, and Jan Paca. "Aerobic degradation of 2,4-dinitrotoluene by individual bacterial strains and defined mixed population in submerged cultures." Journal of Hazardous Materials 192, no. 2 (2011): 605–13. http://dx.doi.org/10.1016/j.jhazmat.2011.05.061.

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Kumar, Gopalakrishnan, Periyasamy Sivagurunathan, Jeong-Hoon Park, et al. "HRT dependent performance and bacterial community population of granular hydrogen-producing mixed cultures fed with galactose." Bioresource Technology 206 (April 2016): 188–94. http://dx.doi.org/10.1016/j.biortech.2016.01.104.

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Rontani, J. F., E. Rambeloarisoa, J. C. Bertrand, and G. Giusti. "Degradation of alkyl-substituted benzenes and their photo-oxidation products by a marine mixed bacterial population." Marine Environmental Research 16, no. 4 (1985): 301–14. http://dx.doi.org/10.1016/0141-1136(85)90025-x.

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Nocker, Andreas, and Anne K. Camper. "Selective Removal of DNA from Dead Cells of Mixed Bacterial Communities by Use of Ethidium Monoazide." Applied and Environmental Microbiology 72, no. 3 (2006): 1997–2004. http://dx.doi.org/10.1128/aem.72.3.1997-2004.2006.

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ABSTRACT The distinction between viable and dead bacterial cells poses a major challenge in microbial diagnostics. Due to the persistence of DNA in the environment after cells have lost viability, DNA-based quantification methods overestimate the number of viable cells in mixed populations or even lead to false-positive results in the absence of viable cells. On the other hand, RNA-based diagnostic methods, which circumvent this problem, are technically demanding and suffer from some drawbacks. A promising and easy-to-use alternative utilizing the DNA-intercalating dye ethidium monoazide bromi

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Sajjan, Uma, George Thanassoulis, Vera Cherapanov, et al. "Enhanced Susceptibility to Pulmonary Infection withBurkholderia cepacia inCftr−/− Mice." Infection and Immunity 69, no. 8 (2001): 5138–50. http://dx.doi.org/10.1128/iai.69.8.5138-5150.2001.

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ABSTRACT Progressive pulmonary infection is the dominant clinical feature of cystic fibrosis (CF), but the molecular basis for this susceptibility remains incompletely understood. To study this problem, we developed a model of chronic pneumonia by repeated instillation of a clinical isolate of Burkholderia cepacia (genomovar III, ET12 strain), an opportunistic gram-negative bacterium, from a case of CF into the lungs of Cftr m1unc−/−(Cftr −/−) and congenicCftr +/+ controls. Nine days after the last instillation, the CF transmembrane regulator knockout mice showed persistence of viable bacteria

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Kamizela, Tomasz, Anna Grobelak, and Malgorzata Worwag. "Use of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans in the Recovery of Heavy Metals from Landfill Leachates." Energies 14, no. 11 (2021): 3336. http://dx.doi.org/10.3390/en14113336.

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Among the methods used to remove metals and their compounds from landfill leachates with low application costs and high efficiency are bioleaching and biosorption. The most effective bacteria used in the metal removal process are Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The aim of the study was to determine the usefulness of the A. ferrooxidans and A. thiooxidans population in removing heavy metals from landfill leachate. In addition, development opportunities for bacterial population using landfill leachate as growth medium were identified. The substrate for the resea

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Tartakovsky, B., M. J. Levesque, R. Dumortier, R. Beaudet, and S. R. Guiot. "Biodegradation of Pentachlorophenol in a Continuous Anaerobic Reactor Augmented with Desulfitobacterium frappieriPCP-1." Applied and Environmental Microbiology 65, no. 10 (1999): 4357–62. http://dx.doi.org/10.1128/aem.65.10.4357-4362.1999.

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ABSTRACT In this work, a strain of anaerobic pentachlorophenol (PCP) degrader, Desulfitobacterium frappieri PCP-1, was used to augment a mixed bacterial community of an anaerobic upflow sludge bed reactor degrading PCP. To estimate the efficiency of augmentation, the population of PCP-1 in the reactor was enumerated by a competitive PCR technique. The PCP-1 strain appeared to compete well with other microorganisms of the mixed bacterial community, with its population increasing from 106 to 1010 cells/g of volatile suspended solids within a period of 70 days. Proliferation of strain PCP-1 allow

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Lemaire, Benny, Steven Janssens, Erik Smets, and Steven Dessein. "Endosymbiont Transmission Mode in Bacterial Leaf Nodulation as Revealed by a Population Genetic Study of Psychotria leptophylla." Applied and Environmental Microbiology 78, no. 1 (2011): 284–87. http://dx.doi.org/10.1128/aem.06130-11.

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ABSTRACTLeaf-nodulated plants are colonized by vertically inherited bacterial endosymbionts, which maintain symbioses throughout host generations. The permanent character of the interaction implies phylogenetic congruence between the host and the endosymbiont. However, the present population genetic study ofPsychotria leptophyllaprovides evidence for a mixed symbiont transmission involving both vertical inheritance and horizontal transfers from the environment.

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Frigon, D., E. Arnaiz, D. B. Oertherf, and L. Raskin. "Who eats what? Classifying microbial populations based on diurnal profiles of rRNA levels." Water Science and Technology 46, no. 1-2 (2002): 1–9. http://dx.doi.org/10.2166/wst.2002.0448.

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Identifying the relationships between various bacterial populations and the substrates they consume is central to the understanding of population dynamics and to the development of process control in activated sludge. However, linking a heterotrophic population to its activity in situ is difficult because ribosomal RNA (rRNA) techniques, while allowing the rapid identification of populations, provide little information about their heterotrophic activity. Activated sludge models describe biodegradation kinetics by classifying substrates into two types: readily and slowly degradable substrates.

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Zou, Songbao, Qianqian Zhang, and Jun Gong. "Comparative Transcriptomics Reveals Distinct Gene Expressions of a Model Ciliated Protozoan Feeding on Bacteria-Free Medium, Digestible, and Digestion-Resistant Bacteria." Microorganisms 8, no. 4 (2020): 559. http://dx.doi.org/10.3390/microorganisms8040559.

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Bacterivory is an important ecological function of protists in natural ecosystems. However, there are diverse bacterial species resistant to protistan digestion, which reduces the carbon flow to higher trophic levels. So far, a molecular biological view of metabolic processes in heterotrophic protists during predation of bacterial preys of different digestibility is still lacking. In this study, we investigated the growth performance a ciliated protozoan Tetrahymena thermophila cultivated in a bacteria-free Super Proteose Peptone (SPP) medium (control), and in the media mixed with either a dig

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Tetz, George, and Victor Tetz. "Evaluation of a New Culture-Based AtbFinder Test-System Employing a Novel Nutrient Medium for the Selection of Optimal Antibiotics for Critically Ill Patients with Polymicrobial Infections within 4 h." Microorganisms 9, no. 5 (2021): 990. http://dx.doi.org/10.3390/microorganisms9050990.

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Here, we describe the validation of a new phenotypic culture-based AtbFinder method for rapid selection of antibiotics in vitro using specimens with mono- and polybacterial infections. AtbFinder, which can be applied to any type of non-blood tissue, does not require isolation of pure bacterial cultures. The method uses a novel TGV medium that allows more rapid bacterial growth of Gram-positive and Gram-negative monoisolates compared with that achieved with conventional laboratory media, demonstrating overall sensitivity, specificity, PPV, NPV values of 99.6%, 98.1%, 98.5%, and 99.4%, respectiv

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Kikuchi, Yoshitomo, and Joerg Graf. "Spatial and Temporal Population Dynamics of a Naturally Occurring Two-Species Microbial Community inside the Digestive Tract of the Medicinal Leech." Applied and Environmental Microbiology 73, no. 6 (2007): 1984–91. http://dx.doi.org/10.1128/aem.01833-06.

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ABSTRACT The medicinal leech, Hirudo verbana, is one of the simplest naturally occurring models for digestive-tract symbioses, where only two bacterial species, Aeromonas veronii bv. sobria (γ-Proteobacteria) and a Rikenella-like bacterium (Bacteroidetes), colonize the crop, the largest compartment of the leech digestive tract. In this study, we investigated spatial and temporal changes of the localization and microcolony structure of the native symbionts in the crop, after ingestion of a sterile blood meal, by fluorescence in situ hybridization. The population dynamics differed between the tw

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SONG, M. K., and J. J. KENNELLY. "IN SITU DEGRADATION OF FEED INGREDIENTS, FERMENTATION PATTERN AND MICROBIAL POPULATION AS INFLUENCED BY RUMINAL AMMONIA CONCENTRATION." Canadian Journal of Animal Science 69, no. 4 (1989): 999–1006. http://dx.doi.org/10.4141/cjas89-114.

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Effects of ruminal ammonia concentration on ruminal parameters and in situ degradation of feed ingredients were examined with nonlactating Holstein cows fed a complete mixed diet (70% barley silage and 30% concentrate mixture, dry matter (DM) basis). Ruminal ammonia concentrations of 16.3, 24.8 and 34.9 mg 100 mL−1 were achieved by continuous intraruminal infusions of NH4Cl. Ammonia concentrations in control animals were 11.2 mg 100 mL−1. Ruminal fluid pH decreased (P < 0.05) and blood urea N increased (P < 0.05) with NH4Cl infusion. NH4Cl infusion increased (P < 0.05) viable counts o

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Blaustein, Ryan Andrew, Patrick Casey Seed, and Erica Marie Hartmann. "3449 Bacterial biotransformation of chemotherapeutics may promote diversity among the intestinal microbiota." Journal of Clinical and Translational Science 3, s1 (2019): 3. http://dx.doi.org/10.1017/cts.2019.11.

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OBJECTIVES/SPECIFIC AIMS: This study aims to test the hypothesis that bacterial biotransformation of chemotherapeutics promotes gut microbial diversity by enhancing persistence of drug-sensitive taxa. METHODS/STUDY POPULATION: The impacts of doxorubicin on a model community of gut bacteria was investigated in vitro in anaerobic batch culture. The synthetic community was composed of specific members predicted by genomic analysis to be sensitive to the therapeutic (i.e., Clostridium innocuum, Lactobacillus sp.), resistant via putative biotransformation (i.e., Escherichia coli, Klebsiella pneumon

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Wainwright, M., S. Alharbi, and N. C. Wickramasinghe. "How do microorganisms reach the stratosphere?" International Journal of Astrobiology 5, no. 1 (2006): 13–15. http://dx.doi.org/10.1017/s1473550406002825.

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A number of studies have demonstrated that bacteria and fungi are present in the stratosphere. Since the tropopause is generally regarded as a barrier to the upward movement of particles it is difficult to see how such microorganisms can reach heights above 17 km. Volcanoes provide an obvious means by which this could be achieved, but these occur infrequently and any microorganisms entering the stratosphere from this source will rapidly fall out of the stratosphere. Here, we suggest mechanisms by which microorganisms might reach the stratosphere on a more regular basis; such mechanisms are, ho

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Anpilov, A. M., E. M. Barkhudarov, N. Christofi, et al. "The effectiveness of a multi-spark electric discharge system in the destruction of microorganisms in domestic and industrial wastewaters." Journal of Water and Health 2, no. 4 (2004): 267–77. http://dx.doi.org/10.2166/wh.2004.0024.

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The aim of this work was to investigate the effectiveness of a high voltage multi-spark electric discharge, with pulse energy of 1 Joule, in killing microorganisms in wastewater. Wastewater from primary treated effluent arising from domestic and industrial sources was abstracted for continuous pulsed discharge disinfection. The wastewater contained a large mixed population of microorganisms (∼107 CFU ml−1 [109 CFU 100 ml−1] total aerobic heterotrophic bacteria) including vegetative cells and spores. The electrical conductivity of the wastewater ranged from 900–1400 μS cm−1 and it was shown tha

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Avila, Vicente A. Diaz, Venício M. Carvalho, Aylle Medeiros Matos, et al. "PSXI-15 Clove and cashew nut shell oils on the growth of ruminal gram-positive bacteria." Journal of Animal Science 97, Supplement_3 (2019): 407. http://dx.doi.org/10.1093/jas/skz258.807.

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Abstract Livestock production efficiency is dependent on the rumen environment. Daily or long-term alterations of the rumen bacterial population can modify the degradability of feeds and the production of volatile fatty acids. This work was realized to evaluate the effect of clove (Syzygium aromaticum) and cashew nut shell (Anacardium occidentale) oils on the growth of ruminal bacteria Ruminococcus albus, Ruminococcus flavefaciens and Streptococcus bovis, gram-positive bacteria involved on carbohydrate degradation. The experiments were designed using each bacteria exposed to two different dose

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Dexter, Sarah J., Miguel Cámara, Martyn Davies, and Kevin M. Shakesheff. "Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell number from a mixed population." Biomaterials 24, no. 1 (2003): 27–34. http://dx.doi.org/10.1016/s0142-9612(02)00239-9.

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Bathe, Stephan, Norbert Schwarzenbeck, and Martina Hausner. "Bioaugmentation of activated sludge towards 3-chloroaniline removal with a mixed bacterial population carrying a degradative plasmid." Bioresource Technology 100, no. 12 (2009): 2902–9. http://dx.doi.org/10.1016/j.biortech.2009.01.060.

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Marques, Sirlei G., Maria Rosa Q. Bomfim, Conceição de Maria P. S. Azevedo, et al. "Mixed secondary bacterial infection is associated with severe lesions of chromoblastomycosis in a neglected population from Brazil." Diagnostic Microbiology and Infectious Disease 95, no. 2 (2019): 201–7. http://dx.doi.org/10.1016/j.diagmicrobio.2019.05.018.

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Gül, Bahar Yavuztürk, Derya Yüksel Imer, Pyung-Kyu Park, and Ismail Koyuncu. "Selection of quorum quenching (QQ) bacteria for membrane biofouling control: effect of different Gram-staining QQ bacteria, Bacillus sp. T5 and Delftia sp. T6, on microbial population in membrane bioreactors." Water Science and Technology 78, no. 2 (2018): 358–66. http://dx.doi.org/10.2166/wst.2018.305.

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Abstract This study aimed to address the gap in understanding how the microbial community present within quorum quenching-membrane bioreactor (QQ-MBRs) changes during the operations by investigating the behavior of two different types of QQ bacteria, Bacillus sp. T5 and Delftia sp. T6. The anti-biofouling effects of T5 and T6 in the QQ-MBR were 85% and 76%, respectively. According to the Illumina HiSeq results, when the QQ-MBR was operated with Gram-positive bacteria, T5, in the mixed liquor a reduction was observed in Gram-positive bacteria and Gram-negative bacteria population increased. In

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