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Academic literature on the topic 'Modified triple antibiotic paste'
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Journal articles on the topic "Modified triple antibiotic paste"
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Khoshkhounejad, Mehrfam, Mahsa Sobhi Afshar, Fereshteh Jabalameli, Mohammad Emaneini, and Mohammadreza Sharifian. "Cytotoxicity Evaluation of Minimum Antibacterial Values of Different Medicaments Used in Endodontic Regenerative Procedures." European Journal of Dentistry 13, no. 04 (October 2019): 514–20. http://dx.doi.org/10.1055/s-0039-3401369.
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Abstract Objectives This study aimed to evaluate the cytotoxicity of minimum antibacterial values of medicaments used in endodontic regeneration on stem cells. Materials and Methods “Minimum inhibitory concentration,” “minimum bactericidal concentration,” and “minimum biofilm inhibitory concentration” of triple and double antibiotic paste, a modified triple antibiotic paste (minocycline replaced by clindamycin), Augmentin, and calcium hydroxide were determined using Enterococcus faecalis (ATCC 29212) by microtiter plate method. Direct cytotoxic effects of drugs were evaluated by lactate dehydrogenase and water-soluble tetrazolium salt-1 assays using stem cells of apical papilla obtained from immature third molars via enzymatic digestion. Statistical Analysis Data were analyzed using IBM SPSS Statistics 24, one-way analysis of variance and post hoc comparisons. The statistical power was set at p < 0.05. Results All medicaments caused similar cytotoxicity and cell proliferation at “minimum inhibitory concentration” (p > 0.05) except Augmentin which was significantly more toxic than others (p < 0.05). At “minimum bactericidal concentration,” calcium hydroxide was more toxic than other drugs (p < 0.001), but its adverse effect on cell proliferation was the same as Augmentin (p > 0.05). Triple and double antibiotic paste revealed similar favorable effects in terms of toxicity and proliferation rate at most of the tested concentrations (p > 0.05). At “minimum biofilm inhibitory concentration” both the modified paste and Augmentin caused less proliferation rate than triple and double antibiotic paste (p < 0.001and p < 0.05, respectively) and Augmentin induced more cytotoxicity (p < 0.05). Conclusions Considering the antimicrobial potency, triple antibiotic paste seems to be the safest drug for the stem cells of apical papilla, while Augmentin may have some adverse effects.
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Ojha Tiwari, Urvashi, Afsana Begum, Jyoti Jain, Sandhya Yadav, and Nurez Anwar. "COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF TRIPLE ANTIBIOTIC PASTE AND MODIFIED DOUBLE ANTIBIOTIC PASTE USING DIFFERENT VEHICLES AGAINST CANDIDA ALBICANS." International Journal of Advanced Research 8, no. 1 (January 31, 2020): 528–33. http://dx.doi.org/10.21474/ijar01/10325.
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Asnaashari, Mohammad, Mohammad Jafar Eghbal, Amirali Sahba Yaghmayi, Mehdi Shokri, and Saranaz Azari-Marhabi. "Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study." Journal of Lasers in Medical Sciences 10, no. 5 (December 1, 2019): S23—S29. http://dx.doi.org/10.15171/jlms.2019.s5.
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Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.
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Akman, Melek, Makbule Bilge Akbulut, Hale Arı Aydınbelge, and Sema Belli. "Comparison of Different Irrigation Activation Regimens and Conventional Irrigation Techniques for the Removal of Modified Triple Antibiotic Paste from Root Canals." Journal of Endodontics 41, no. 5 (May 2015): 720–24. http://dx.doi.org/10.1016/j.joen.2015.01.001.
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Kumar, Amit, Vijay Parashar, Shabab A. Khan, Puja Singh, Shishir Sharma, and Kumar Anand. "Effect of Intracanal Medicaments (Modified Triple Antibiotic Paste, Calcium Hydroxide, and Aloe Vera) on Microhardness of Root Dentine: An In Vitro Study." Journal of Contemporary Dental Practice 21, no. 6 (2020): 632–35. http://dx.doi.org/10.5005/jp-journals-10024-2813.
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Singhal, Somya, Meenakshi Venkataraman, AseemP Tikku, and Anil Chandra. "Comparative analysis of tooth discoloration induced by conventional and modified triple antibiotic pastes used in regenerative endodontics." Indian Journal of Dental Research 30, no. 6 (2019): 933. http://dx.doi.org/10.4103/ijdr.ijdr_782_18.
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Paulindraraj, Shankar, Hemalatha Ramkumar, Karthika Shankar, Senthil Dhakshinamoorthy, Trophimus G. Jayakaran, and Charishma S. Bommareddy. "Comparison of Modified Triple Antibiotic Paste in Two Concentrations for Lesion Sterilization and Tissue Repair in Primary Molars: An In Vivo Interventional Randomized Clinical Trial." International Journal of Clinical Pediatric Dentistry 14, no. 3 (September 17, 2021): 388–92. http://dx.doi.org/10.5005/jp-journals-10005-1951.
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Alrahman, Mewan Salahalddin A., Bestoon Muhammed Faraj, and Kawa F. Dizaye. "Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics." BioMed Research International 2020 (February 18, 2020): 1–13. http://dx.doi.org/10.1155/2020/2128473.
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Background and Objectives. Multiple antibacterial agents have been mixed and used as an intracanal medicament-like modified triple antibiotic paste (MTAP) to eliminate Enterococcus faecalis (EF), which has been most frequently identified in the cases of failed root canal treatment and periapical lesions. This study is aimed at using a single antibacterial agent, nitrofurantoin (Nit), as an experimental intracanal medicament paste against different clinical isolates of EF bacteria and at comparing its antimicrobial efficacy with MTAP. Materials and Methods. Three strains of EF (S1, S2, and S3) were clinically isolated. A total of 198 straight single-rooted human teeth were collected and divided randomly into three main groups: group N (Nit) (n=90), group M (MTAP) (n=90), and group W (distilled water) (n=18). The main groups were subdivided into three subgroups according to the strain of EF: in groups N and M, subgroups S1, S2, and S3 (n=30), while in group W, subgroups S1, S2, and S3 (n=6). Then, each subgroup of N and M was divided into five groups (n=6) according to the concentrations of Nit or MTAP (6.25, 12.5, 25, 50, and 100 mg/mL). The colony-forming unit (CFU) of EF from the canal lumen and dentinal chips was measured. Results. Nit could eradicate S1, S2, and S3 completely with concentrations of 6.25, 12.5, and 25 mg/mL, respectively, while MTAP showed complete eradication of the three strains only at 25 mg/mL. In all the groups, it was found that the CFU counts of EF in the dentinal chips were higher than those in the root canal lumen. Conclusion. At the concentration of 25 mg/mL, the Nit paste is effective in eradicating EF completely when it is used as an intracanal medicament.
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El-Baz, Alaa, and Geraldine Ahmed. "COMPARISON OF THE EFFECT OF CALCIUM HYDROXIDE AND THE MODIFIED TRIPLE ANTIBIOTIC PASTE AS INTRACANAL MEDICATION ON THE ELIMINATION OF ENTEROCOCCUS FAECALIS IN RETREATMENT CASES A RANDOMIZED DOUBLE BLIND CLINICAL TRIAL." Egyptian Dental Journal 63, no. 4 (October 1, 2017): 3995–4004. http://dx.doi.org/10.21608/edj.2017.76466.
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El-Baz, Alaa, and Geraldine Ahmed. "COMPARISON OF THE EFFECT OF CALCIUM HYDROXIDE AND THE MODIFIED TRIPLE ANTIBIOTIC PASTE AS INTRACANAL MEDICATION ON THE ELIMINATION OF ENTEROCOCCUS FAECALIS IN RETREATMENT CASES. A RANDOMIZED DOUBLE BLIND CLINICAL TRIAL." Egyptian Dental Journal 63, no. 4 (October 1, 2017): 3683–92. http://dx.doi.org/10.21608/edj.2017.92784.
Full textDissertations / Theses on the topic "Modified triple antibiotic paste"
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Stiles, Spencer R. "The effects on the antimicrobial properties of Hoshino's triple antibiotic paste when chlorhexidine gluconate (0.12%) is substituted for the propylene glycol and macrogol ointment mixture." Morgantown, W. Va. : [West Virginia University Libraries], 2010. http://hdl.handle.net/10450/11207.
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Thesis (M.S.)--West Virginia University, 2010.Title from document title page. Document formatted into pages; contains v, 47 p. : col. ill. Includes abstract. Includes bibliographical references (p. 44-47).
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Prather, Blake. "Effect of Antibiotic Pastes on Chemical Structure and Microhardness of Radicular Dentin." Thesis, 2014. http://hdl.handle.net/1805/6503.
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Indiana University-Purdue University Indianapolis (IUPUI)Introduction: Regenerative endodontic therapy in immature teeth with necrotic pulps triggers continued root development, thereby improving the prognosis of these teeth. Disinfection of the canal is accomplished with an intracanal medicament, such as triple antibiotic paste (TAP) composed of metronidazole, ciprofloxacin, and minocycline. A modified triple antibiotic paste (MTAP) that replaces minocycline with clindamycin has recently been suggested to avoid the tooth discoloration and potential demineralization from minocycline. The effect these pastes have on radicular dentin is unknown. Objectives: The aim of this study was to investigate the effects of two intracanal medicaments used during endodontic regeneration, TAP and MTAP, at concentrations of 1 g/mL and 1 mg/mL, on the microhardness and chemical structure of radicular dentin. Materials and Methods: Roots from extracted, unrestored, non-carious human premolar teeth were sectioned. An antibiotic paste (MTAP or TAP) or sterile water (control) was applied to treatment groups and stored for four weeks in 80-percent humidity at 37 °C. The effect of each paste on the microhardness of radicular dentin was measured using a Vickers Microhardness Tester (n = 17) to take three pretreatment and post-treatment measurements at both 500 µm and 1000 µm from the pulp-dentin interface. The chemical structure was assessed from dentin specimens treated with the same medicaments or sterile water for four weeks. After treatment, three measurements were taken on each specimen using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy to measure the phosphate/amide I ratios of dentin (n = 7). Results: The 1 g/mL of TAP or MTAP and the 1 mg/mL methylcellulose-based TAP caused significant reduction in microhardness of roots compared with untreated control roots at 500 µm and 1000 µm from the pulp-dentin interface. Furthermore, the methylcellulose-based 1 mg/mL TAP and MTAP caused significantly less reduction in microhardness compared with 1 g/mL TAP and MTAP. The 1 g/mL of TAP and DAP caused significantly lower phosphate/amide I ratios compared with other groups. Conclusion: The use of methylcellulose based 1 mg/mL of TAP and MTAP may minimize the reduction in microhardness of roots compared with the currently used 1 g/mL concentration of these antibiotics.
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Alyas, Sarmad Mazin. "The Longevity of Residual Antibacterial Effect of Dentin Treated with Various Concentrations of Triple Antibiotic Paste." Thesis, 2016. http://hdl.handle.net/1805/10235.
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Indiana University School of Dentistry
Department of EndodonticsIntroduction: Triple antibiotic paste (TAP, 1000 mg/ml) is composed of equal portions of ciprofloxacin, metronidazole and minocycline and is used as an intracanal dressing to disinfect the infected immature root canal during endodontic regeneration procedures. Lower concentrations of TAP have been recommended to minimize detrimental effects on pulp stem cells. TAP can be retained within the dentin matrix and its continual release confers an antibacterial effect to the dentin. Objective: The aim of this in vitro study was to investigate the residual antibacterial effect of dentin treated with various concentrations of TAP loaded into a gel system. Materials and Methods: Radicular dentin slabs were prepared from human teeth after obtaining IRB approval. The slabs were sterilized and treated with methylcellulose-based TAP of 100 mg/mL, 10 mg/mL, 1 mg/mL, 1.5% NaOCl, placebo paste with no TAP, or a positive control group with pure 1000 mg/mL TAP. Samples in each group were treated with the assigned TAP concentration for three weeks or immersed in 1.5% NaOCl for five minutes (n =18 per group). All samples were then irrigated with sterile water followed by 17% EDTA and incubated in phosphate buffered saline for either 2 or 4 weeks. Samples were then inoculated with Enterococcus faecalis and incubated for an additional 3 weeks. Biofilm formed on each sample was then dislodged and spiral plated to evaluate the bacterial colony-forming units. Data were analyzed using Fisher’s Exact tests and Wilcoxon rank sum tests (α = 0.05). Results: Dentin treated with 10, 100, or 1000 mg/mL of TAP demonstrated significant residual antibacterial effects up to four weeks. However, only 100 mg/mL TAP was able to completely prevent bacterial colonization after four weeks. No considerable residual antibacterial effect was observed in dentin treated with placebo gel, 1 mg/ml TAP or 1.5% NaOCl. Conclusion: At least 10 mg/mL of TAP loaded into a methylcellulose system is required to achieve a substantial residual antibacterial effect for four weeks.
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Azabi, Asma Abulqasem. "The antimicrobial efficacy of innovative 3D triple antibiotic paste-mimic tubular scaffold against actinomyces naeslundii." Thesis, 2015. http://hdl.handle.net/1805/8043.
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Indiana University-Purdue University Indianapolis (IUPUI)Background: Root canal disinfection is an essential requirement for the success of regenerative endodontics. Currently, the so-called triple antibiotic paste (TAP) is considered the standard of care. Notwithstanding the good antimicrobial capacity, the high concentration of TAP has shown significant toxicity to human cells, especially dental pulp stem cells. A novel drug release system, i.e., a triple antibiotic paste-mimic electrospun scaffold containing low concentrations of the antibiotics present in the TAP, has emerged as an effective and reliable alternative to fight root canal infections without potential toxic effects on dental stem cells, which are an integral part of the regenerative treatment. Objectives: The aim of this study was to determine the antimicrobial efficacy of an innovative three-dimensional (3D) triple antibiotic paste-mimic tubular scaffold against Actinomyces naeslundii biofilm formed inside human root canal dentinal tubules. Materials and methods: Pure polydioxanone (PDS) polymer solution and PDS loaded with metronidazole, ciprofloxacin and minocycline (35 wt.% of each antibiotic, 3D-TAP-mimic scaffold) were spun into 3D fibrous scaffolds. A. naeslundii (ATCC 43146) was centrifuged to induce biofilm formation inside human root canal dentinal tubules using a dentin slice model (1 mm thickness and 2.5 mm canal diameter). The infected dentin slices were exposed to the 3D-TAP-mimic scaffold, TAP solution (50 mg/mL of each antibiotic), and antibiotic-free PDS. Biofilm elimination was quantitatively and qualitatively analyzed by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), respectively. Results: A dense penetration of A. naeslundii biofilm was observed by CLSM throughout the dentinal tubules. 3D-TAP-mimic scaffold significantly reduced the percentage of viable bacteria compared with PDS (p <.05). TAP solution completely eliminated viable bacteria without differing from 3D-TAP-mimic scaffolds. SEM images showed results similar to CLSM. Conclusion: Collectively, the proposed tubular 3D-TAP-mimic scaffold holds significant clinical potential for root canal disinfection strategy prior to regenerative endodontics.
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Nerness, Andrew. "The effect of triple antibiotic paste and EDTA on the surface loss and surface roughness of radicular dentin." Thesis, 2014. http://hdl.handle.net/1805/6505.
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Indiana University-Purdue University Indianapolis (IUPUI)Introduction: Regenerative endodontic therapy in immature teeth with necrotic pulps triggers continued root development thereby improving the prognosis of these teeth. Several agents are under consideration for the disinfection and conditioning phases of this therapy. Triple antibiotic paste (TAP, i.e. equal parts of ciprofloxacin, metronidazole, minocycline) is used for canal disinfection and 17% EDTA solution is used for dentin conditioning. However, TAP and EDTA cause demineralization and their effect on surface loss and surface roughness of radicular dentin during regenerative procedures has not been quantified. Surface loss may be correlated with reduced tooth strength and surface roughness may be correlated with stem cell attachment. Objectives: The aim of this in vitro study was to quantitatively investigate the surface loss and surface roughness on human radicular dentin after treatment with two concentrations of TAP followed by EDTA. Materials and Methods: Human radicular dentin specimens were prepared from extracted human anterior teeth and randomized into six experimental groups. Group 1: saline control; Group 2: 17% EDTA; Group 3: TAP 1 mg/mL; Group 4: TAP 1 mg/mL and 17% EDTA; Group 5: TAP 1,000 mg/mL; Group 6: TAP 1,000 mg/mL and 17% EDTA for 5 minutes. After TAP is applied to Groups 3-6, all groups were incubated for 4 weeks. Then, groups 2, 4, and 6 were treated with EDTA for 5 minutes. Dentin surface loss (μm) and surface roughness (Ra, μm) were quantified after various treatments using non-contact and contact profilometry, respectively. Data were analyzed by one-way analysis of variance (α = 0.05) Hypothesis: It was hypothesized that there would be a significant difference in surface loss or surface roughness between at least two treatment groups. Results: All treatment groups showed significantly higher surface loss compared to untreated control. Dentin treated with 1g/mL TAP caused significant increase in surface loss and surface roughness compared to dentin treated with 1 mg/mL TAP. However, only 1g/mL TAP treated dentin showed significantly higher surface roughness compared to untreated control. The use of EDTA after both concentrations of TAP did not have significant additive effect on surface loss and surface roughness of dentin. Conclusion: The use of 1 mg/mL TAP can minimize surface loss and surface roughness of radicular dentin compared to higher concentrations. The use of EDTA after TAP may not cause additional surface loss and surface roughness of dentin.
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Novella, Victoria. "Efeito da medicação intracanalar nas propriedades dos cimentos biocerâmicos: revisão narrativa." Master's thesis, 2020. http://hdl.handle.net/10284/9505.
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Os medicamentos intracanalares como o hidróxido de cálcio e as pastas dupla (DAP) e
tripla antibióticas (TAP) podem ser usados em várias condições endodônticas, e deveriam
ser removidos antes da colocação dos materiais obturadores. O agregado de trióxido
mineral (MTA) e a Biodentine® estão entre os materiais os mais populares. Este trabalho
tem por objetivo fazer uma revisão narrativa bibliográfica dos efeitos da medicação
intracanalar nas propriedades dos cimentos biocerâmicos à luz dos conhecimentos atuais.
Foi realizada uma pesquisa nos motores de busca Pubmed, B-On e Google Scholar
utilizando como palavras-chave “intracanal medicaments”, “bioceramic sealers”,
“calicum silicate sealer”, “bioceramic endodontic cement”, “Mineral trioxide
aggregate”, “Biodentine”, “Triple antibiotic paste”,” double antibiotic paste”,
“Calicum hydroxyde”. Os autores não demostraram resultados concordantes, sobretudo
para o hidróxido de cálcio, na resistência ao deslocamento, isto é, a resistência desses
materiais quando expostos às forças oclusais ou procedimentos operatórios. A maioria
dos estudos demostraram que as pastas triplas antibióticas e duplas antibióticas reduzem
a força de adesão do MTA e da Biodentine®. A adaptação marginal do MTA aumenta
com a aplicação de medicação de hidróxido de cálcio. Existem ainda muitas questões por
demonstrar, no que diz respeito a este tema, sendo necessários mais estudos clínicos para
se conseguir concluir com mais clareza quais os efeitos dos medicamentos intracanalares
sobre as propriedades dos cimentos biocerâmicos.Intracanal medications like calcium hydroxide, the double (DAP) and triple antibiotics pastes (TAP) can be used in various endodontic conditions and should be removed before any placement of filling or restorative materials. Mineral trioxide aggregate (MTA) and Biodentine are among the most popular materials. This work aims to make a bibliographic narrative review of the effects of intracanal canal medication on the properties of bioceramics cements in the light of current knowledge. A search was performed on the search engines Pubmed, B-On and Google Scholar using as keywords “intracanal medicaments”, “bioceramic sealers”, “calicum silicate sealer”, “bioceramic endodontic cement”, “Mineral trioxide aggregate”, “Biodentine "," Triple antibiotic paste",”,”double antibiotic paste”" Calicum hydroxyde ". The authors did not demonstrate concordant results, especially for calcium hydroxide, on the push-out bond strength. The push-out bond strength is the force needed for the displacement of the dental material tested under mechanical procedure or occlusal forces. The majority of the studies have shown that triple antibiotic pastes and double antibiotic pastes reduce the bond strength of MTA and Biodentine®. The marginal adaptation of the MTA is improved with calcium hydroxide. The studies are not completely conclusive, still exist various questions about this theme and further clinical studies are needed to see the effects of intracanal canal medications on the properties of bioceramics cements.
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Yassen, Ghaeth H. "The effect of endodontic regeneration medicaments on mechanical properties of radicular dentin." Thesis, 2013. http://hdl.handle.net/1805/3226.
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Indiana University-Purdue University Indianapolis (IUPUI)Endodontic regeneration treatment of necrotic immature teeth has gained popularity in recent years. The approach suggests a biological alternative to induce a continuous root development. In this project, three in vitro experiments were conducted to investigate the effect of three medicaments used in endodontic regeneration on mechanical properties and chemical structure of radicular dentin. In the first experiment, we investigated longitudinally the effect of medicaments on the indentation properties of the root canal surface of immature teeth using a novel BioDent reference point indenter. A significant difference in the majority of indentation parameters between all groups was found after one-week and one-month application of medicaments (p<0.0001): triple antibiotic paste (TAP) > double antibiotic paste (DAP) > control > calcium hydroxide [Ca(OH)2]. The four-week exposure of dentin to TAP and DAP caused 43% and 31% increase in total indentation distance outcome, respectively. In the second experiment, we investigated longitudinally the effect of medicaments on the chemical structure of immature radicular dentin by measuring the phosphate/amide I ratios of dentin using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy. Phosphate/amide I ratios were significantly different between the four groups after one week, two weeks and four week application of medicaments (p<0.0001): Ca(OH)2-treated dentin > untreated dentin > DAP-treated dentin > TAP-treated dentin. In the third experiment, we investigated longitudinally the effect of medicaments on root fracture resistance and microhardness of radicular dentin. For the microhardness, the two-way interaction between group and time was significant (p<0.001). TAP and DAP caused a significant and continuous decrease in dentin microhardness after one and three month application, respectively. The three-month intracanal application of Ca(OH)2 significantly increased the microhardness of root dentin. The time factor had a significant effect on fracture resistance (p<0.001). All medicaments caused significant decrease in fracture resistance ranging between 19%-30% after three month application compared to one week application. The three medicaments used in endodontic regeneration caused significant change in the chemical integrity of the superficial radicular dentin and significantly affected the indentation properties of the root canal surface. Furthermore, the three month intracanal application of medicaments significantly reduced the fracture resistance of roots.
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Sabrah, Ala'a Hussein Aref 1984. "Diluted antibiotics for treating traumatized immature teeth." Thesis, 2014. http://hdl.handle.net/1805/6500.
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Indiana University-Purdue University Indianapolis (IUPUI)Endodontic regeneration (ERP) has been successfully used in the treatment of traumatized immature teeth. The procedure has three essential steps: disinfecting the root canal (i.e. triple antibiotic paste (TAP) or double antibiotic paste (DAP)), provoking bleeding inside the canal to form a scaffold upon which pulp stem cells will be deposited and continue root growth, and creating a good coronal seal. Previous research has reported that antibiotic pastes (TAP and DAP) are cytotoxic to stem cells in the concentrations commonly used in endodontic regeneration (1000 mg/mL). To decrease the adverse effects on stem cells and increase the rate of success of the regeneration, defining appropriate antibiotic concentrations for ERP is critical. In this project, five in-vitro experiments were conducted to determine the breakpoint dilutions of both TAP and DAP medicaments, and to prepare a suitable novel pastes containing diluted TAP or DAP medicaments for ERP. In the first experiment, we compared the antibacterial effect of TAP, and DAP against early biofilm formation of Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis bacteria. In the second study, we investigated the antibacterial effect of various dilutions of TAP and DAP antibiotic medicaments against established E. faecalis biofilm. In the third experiment, we investigated longitudinally the residual antibacterial activity of human radicular dentin treated with 1000, 1 or 0.5 mg/ml of TAP and DAP. In the fourth study, we investigated the cytotoxic effect of various dilutions of TAP and DAP antibiotic medicaments on the survival of human dental pulp stem cells (DPSC). And in the fifth experiment, we investigated the antibacterial and cytotoxic effect of novel intracanal medicaments consisting of methylcellulose (MC) and/or propylene glycol (PG) mixed with 1mg/ml of TAP or DAP. 1 mg/ml of DAP or TAP medicaments had a significant antibacterial effect against early bacterial biofilm formation, and established bacterial biofilm. Furthermore, 1 mg/ml had a residual antibacterial activity comparable to 1000 mg/ml. The novel intracanal medicaments had comparable antibacterial effect to currently used medicaments (1000 mg/ml). Additionally, the novel intracanal medicaments significantly enhanced DPSC metabolic activity, compared to currently used medicaments in endodontic regeneration procedures.