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1

Stark, Oliver, Marius Eckert, Albertus Johannes Malan, and Sören Hohmann. "Fractional Systems’ Identification Based on Implicit Modulating Functions." Mathematics 10, no. 21 (2022): 4106. http://dx.doi.org/10.3390/math10214106.

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This paper presents a new method for parameter identification based on the modulating function method for commensurable fractional-order models. The novelty of the method lies in the automatic determination of a specific modulating function by controlling a model-based auxiliary system, instead of applying and parameterizing a generic modulating function. The input signal of the model-based auxiliary system used to determine the modulating function is designed such that a separate identification of each individual parameter of the fractional-order model is enabled. This eliminates the shortcom
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2

Liu, Da-Yan, and Taous-Meriem Laleg-Kirati. "Robust fractional order differentiators using generalized modulating functions method." Signal Processing 107 (February 2015): 395–406. http://dx.doi.org/10.1016/j.sigpro.2014.05.016.

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3

David, Pumaricra Rojas, Noack Matti, Reger Johann, and Pérez-Zúñiga Gustavo. "State Estimation for Coupled Reaction-Diffusion PDE Systems Using Modulating Functions." Sensors 2022 22, no. 13 (2022): 5008. https://doi.org/10.3390/s22135008.

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Many systems with distributed dynamics are described by partial differential equations (PDEs). Coupled reaction-diffusion equations are a particular type of these systems. The measurement of the state over the entire spatial domain is usually required for their control. However, it is often impossible to obtain full state information with physical sensors only. For this problem, observers are developed to estimate the state based on boundary measurements. The method presented applies the so-called modulating function method, relying on an orthonormal function basis representation. Auxiliary sy
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4

Tarasenko, Anna. "Method of SAR sounding signals investigation and digital forming." ITM Web of Conferences 30 (2019): 15030. http://dx.doi.org/10.1051/itmconf/20193015030.

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5

Fedele, Giuseppe, and Loredana Coluccio. "A recursive scheme for frequency estimation using the modulating functions method." Applied Mathematics and Computation 216, no. 5 (2010): 1393–400. http://dx.doi.org/10.1016/j.amc.2010.02.039.

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6

Pumaricra Rojas, David, Matti Noack, Johann Reger, and Gustavo Pérez-Zúñiga. "State Estimation for Coupled Reaction-Diffusion PDE Systems Using Modulating Functions." Sensors 22, no. 13 (2022): 5008. http://dx.doi.org/10.3390/s22135008.

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Many systems with distributed dynamics are described by partial differential equations (PDEs). Coupled reaction-diffusion equations are a particular type of these systems. The measurement of the state over the entire spatial domain is usually required for their control. However, it is often impossible to obtain full state information with physical sensors only. For this problem, observers are developed to estimate the state based on boundary measurements. The method presented applies the so-called modulating function method, relying on an orthonormal function basis representation. Auxiliary sy
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7

Preisig, H. A., and D. W. T. Rippin. "Theory and application of the modulating function method—II. algebraic representation of Maletinsky's spline-type modulating functions." Computers & Chemical Engineering 17, no. 1 (1993): 17–28. http://dx.doi.org/10.1016/0098-1354(93)80002-5.

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8

Byrski, Witold, and Michał Drapała. "On-line process identification using the Modulating Functions Method and non-asymptotic state estimation." Archives of Control Sciences 32, no. 3 (2022): 535–55. https://doi.org/10.24425/acs.2022.142845.

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The paper presents an iterative identification method dedicated for industrial processes. The method consists of two steps. In the first step, a MISO system is identified with the Modulating Functions Method to obtain sub-models with a common denominator. In the second step, the obtained subsystems are re-identified. This procedure enables to obtain the set of models with different denominators of the transfer functions. The algorithm was used for on-line identification of a glass conditioning process. Identification window is divided into intervals, in which the models can be updated based on
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9

He, Shanghong. "PARAMETER ESTIMATION OF LINEAR CONTINUOUS-TIME DYNAMIC SYSTEM USING MODULATING FUNCTIONS METHOD." Chinese Journal of Mechanical Engineering 39, no. 12 (2003): 129. http://dx.doi.org/10.3901/jme.2003.12.129.

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10

Fedele, G., C. Picardi, and D. Sgro. "A Power Electrical Signal Tracking Strategy Based on the Modulating Functions Method." IEEE Transactions on Industrial Electronics 56, no. 10 (2009): 4079–87. http://dx.doi.org/10.1109/tie.2009.2028352.

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11

Preisig, H. A., and D. W. T. Rippin. "Theory and application of the modulating function method—I. Review and theory of the method and theory of the spline-type modulating functions." Computers & Chemical Engineering 17, no. 1 (1993): 1–16. http://dx.doi.org/10.1016/0098-1354(93)80001-4.

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12

Byrski, Witold, Michał Drapała, and Jȩdrzej Byrski. "An Adaptive Identification Method Based on the Modulating Functions Technique and Exact State Observers for Modeling and Simulation of a Nonlinear Miso Glass Melting Process." International Journal of Applied Mathematics and Computer Science 29, no. 4 (2019): 739–57. http://dx.doi.org/10.2478/amcs-2019-0055.

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Abstract The paper presents new concepts of the identification method based on modulating functions and exact state observers with its application for identification of a real continuous-time industrial process. The method enables transformation of a system of differential equations into an algebraic one with the same parameters. Then, these parameters can be estimated using the least-squares approach. The main problem is the nonlinearity of the MISO process and its noticeable transport delays. It requires specific modifications to be introduced into the basic identification algorithm. The mai
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13

Co, Tomas B., and Sridhar Ungarala. "Batch scheme recursive parameter estimation of continuous-time systems using the modulating functions method." Automatica 33, no. 6 (1997): 1185–91. http://dx.doi.org/10.1016/s0005-1098(97)00020-4.

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14

Asiri, Sharefa, and Taous-Meriem Laleg-Kirati. "Modulating functions-based method for parameters and source estimation in one-dimensional partial differential equations." Inverse Problems in Science and Engineering 25, no. 8 (2016): 1191–215. http://dx.doi.org/10.1080/17415977.2016.1240793.

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15

Tian, Yang, Yan-Qiao Wei, Da-Yan Liu, and Driss Boutat. "Fast and robust estimation for positions and velocities from noisy accelerations using generalized modulating functions method." Mechanical Systems and Signal Processing 133 (November 2019): 106270. http://dx.doi.org/10.1016/j.ymssp.2019.106270.

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16

Drapała, Michał, and Witold Byrski. "Online continuous-time adaptive predictive control of the technological glass conditioning process." Archives of Control Sciences 32, no. 4 (2022): 755–82. https://doi.org/10.24425/acs.2022.143670.

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Glass production has a great industrial importance and is associated with many technological challenges. Control related problems concern especially the last part of the process, so called glass conditioning. Molten glass is gradually cooled down in a long ceramic channels called forehearths during glass conditioning. The glass temperature in each zone of the forehearth should be precisely adjusted according to the assumed profile. Due to cross-couplings and unmeasured disturbances, traditional control systems based on PID controllers, often do not ensure sufficient control quality. This probl
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17

Liu, Yuqi, Nan Qiu, Zhihao Zhang, Yang Li, and Chao Bian. "Detection of Ammonia Nitrogen in Neutral Aqueous Solutions Based on In Situ Modulation Using Ultramicro Interdigitated Array Electrode Chip." Chemosensors 13, no. 4 (2025): 138. https://doi.org/10.3390/chemosensors13040138.

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In this study, an in situ electrochemical modulation method based on an ultramicro interdigitated array electrode (UIAE) sensor chip was developed for the detection of ammonia nitrogen (NH3-N) in neutral aqueous solutions. One comb of the UIAE was used as the working electrode for both the modulating and sensing functions, while the other comb was used as the counter electrode. Utilizing its enhanced mass transfer and proximity effects, the feasibility of in situ modulation of the solution environment near the UIAE chip to generate an electrochemical response for NH3-N was investigated using e
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18

Daniel-Berhe, S., and H. Unbehauen. "Physical parameters estimation of the nonlinear continuous-time dynamics of a DC motor using Hartley modulating functions method." Journal of the Franklin Institute 336, no. 3 (1999): 481–501. http://dx.doi.org/10.1016/s0016-0032(98)00043-x.

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19

Lundberg, Karl-Ola. "An Alternative Method for Measurement of Airborne Sound Insulation." Building Acoustics 8, no. 1 (2001): 57–74. http://dx.doi.org/10.1260/1351010011501731.

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A method for determination of the transmission coefficient from Complex Modulation Transfer Functions CMTF:s based on measured impulse-responses is shown. In the method a separate measurement of the equivalent sound absorption area is not needed in contrast to in the standardised measurement. By averaging over a number of estimates of the impulse-response the influence of background noise can be reduced substantially, implying that low-power sources can be used. A model for the power balance in the receiving room with time-varying power is considered. In the model the quotient of the receiving
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20

Li, Lixia, Yu Shi, Xulei Nan, et al. "Focusing control study of flexural waves by tunable radial metasurfaces." Physica Scripta 100, no. 6 (2025): 065982. https://doi.org/10.1088/1402-4896/add96c.

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Abstract Tunable metasurfaces show promising applications in modulating flexural waves, but existing research lacks effective modulation of multidirectional flexural waves. In this paper, a tunable radial metasurface is proposed, which is able to manipulate the flexural waves to achieve various functions, especially the control of the single-focus and multi-focus focusing positions inside the metasurface. The tunable radial metasurface consists of arc sub-units, and the height of the slider in the arc sub-units can be adjusted to obtain a phase shift covering a range of 2 π with a high transmi
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21

Zhou, Guang-Dong, You-Liang Ding, and Ai-Qun Li. "Wavelet-Based Methodology for Evolutionary Spectra Estimation of Nonstationary Typhoon Processes." Mathematical Problems in Engineering 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/870420.

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Closed-form expressions are proposed to estimate the evolutionary power spectral density (EPSD) of nonstationary typhoon processes by employing the wavelet transform. Relying on the definition of the EPSD and the concept of the wavelet transform, wavelet coefficients of a nonstationary typhoon process at a certain time instant are interpreted as the Fourier transform of a new nonstationary oscillatory process, whose modulating function is equal to the modulating function of the nonstationary typhoon process multiplied by the wavelet function in time domain. Then, the EPSD of nonstationary typh
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22

Byrski, Witold, and Jęedrzej Byrski. "The role of parameter constraints in EE and OE methods for optimal identification of continuous LTI models." International Journal of Applied Mathematics and Computer Science 22, no. 2 (2012): 379–88. http://dx.doi.org/10.2478/v10006-012-0028-3.

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The role of parameter constraints in EE and OE methods for optimal identification of continuous LTI modelsThe paper presents two methods used for the identification of Continuous-time Linear Time Invariant (CLTI) systems. In both methods the idea of using modulating functions and a convolution filter is exploited. It enables the proper transformation of a differential equation to an algebraic equation with the same parameters. Possible different normalizations of the model are strictly connected with different parameter constraints which have to be assumed for the nontrivial solution of the op
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23

Laplante, Véronique, Ulysse Nadeau, Marine Rousseau, Sylvie Lesage, Jean-François Gauchat, and Sarah Pasquin. "Detection of Cardiotrophin-like Cytokine Factor 1 (CLCF1) by flow cytometry." Journal of Immunology 206, no. 1_Supplement (2021): 22.12. http://dx.doi.org/10.4049/jimmunol.206.supp.22.12.

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Abstract Cardiotrophin-like Cytokine Factor 1 (CLCF1) belongs to the IL6 family of cytokines and possesses pro-neurotrophic and immuno-modulating functions. Coding mRNA for CLCF1 has been detected in primary and secondary lymphoid organs (i.e. lymph nodes, spleen and bone marrow), as well as in the lungs and feminine reproductive organs. Modulation of CLCF1’s mRNA levels has been associated with the Th17 polarization in CD4+ T cells. However, little information is available regarding CLCF1 protein levels in these tissues or the nature of the immune cells responsible for its production. This ca
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24

Mykhailenko, Vladyslav, Vadym Svyatnenko, Yuliya Chunyak, and Vladyslav Bachynskiy. "Research of processes in a converter with four-zone voltage regulation and electromechanical load." Bulletin of NTU "KhPI". Series: Problems of Electrical Machines and Apparatus Perfection. The Theory and Practice, no. 1 (5) (May 28, 2021): 39–41. http://dx.doi.org/10.20998/2079-3944.2021.1.07.

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The new mathematical models of the electromagnetic processes is designed in three-phase electric circuit of the semiconductor converters with many-server zoned regulation phases voltages disregarding losses in semiconductor commutator for quick estimation of the influence parameter loads on level and form of the output voltage. mathematical model of the semiconductor converter is also used for study of the connecting processes in semiconductor converter with actively-inductive load. Will develop the method multivariable function, what fall into algorithmic equations of the analysis formed and
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25

Asiri, Sharefa, and Taous-Meriem Laleg-Kirati. "Source Estimation for the Damped Wave Equation Using Modulating Functions Method: Application to the Estimation of the Cerebral Blood Flow." IFAC-PapersOnLine 50, no. 1 (2017): 7082–88. http://dx.doi.org/10.1016/j.ifacol.2017.08.1356.

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26

Zhu, Ying, Meiling Xiao, Tangying Kang, et al. "The Role of Inactivation Methods in Shaping Postbiotic Composition and Modulating Bioactivity: A Review." Foods 14, no. 13 (2025): 2358. https://doi.org/10.3390/foods14132358.

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Postbiotics, as the metabolic products and cellular components of probiotics, possess the characteristics of being non-living yet retaining biological activity. Postbiotics have unique advantages such as high stability, good security, and a clear target of action. In recent years, they have attracted extensive attention due to their potential roles in immune regulation, anti-inflammation, antioxidation, antibacterial activity, and improving intestinal health. This article systematically reviews the composition of postbiotics and their diversity in fermented foods, with a focus on the impact of
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27

Ishihara, Seiichiro, Haruna Kurosawa, and Hisashi Haga. "Stiffness-Modulation of Collagen Gels by Genipin-Crosslinking for Cell Culture." Gels 9, no. 2 (2023): 148. http://dx.doi.org/10.3390/gels9020148.

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The stiffness of extracellular matrices (ECMs) is critical for cellular functions. Therefore, modulating the stiffness of ECMs in vitro is necessary to investigate the role of stiffness in cellular phenomena. Collagen gels are widely used for cell culture matrices in vitro. However, modulation of the stiffness in collagen gels for cell culture is challenging owing to the limited knowledge of the method to increase the stiffness while maintaining low cytotoxicity. Here, we established a novel method to modulate collagen gel stiffness from 0.0292 to 12.5 kPa with low cytotoxicity. We prepared co
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Gyurova, Ivayla E., Hannah Than Win, and Stephen N. Waggoner. "Non-canonical functions of adaptive natural killer cells." Journal of Immunology 204, no. 1_Supplement (2020): 154.7. http://dx.doi.org/10.4049/jimmunol.204.supp.154.7.

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Abstract Natural killer (NK) cells are critical for the control of cytomegalovirus (CMV) infections in both mice and humans. Subsets of phenotypically and functionally distinct adaptive NK cells accumulate after CMV infection and potentially represent desirable new targets for a preventive CMV vaccine owing to their putatively greater antiviral functionality. However, our lab and others have described a series of non-canonical functions of NK cells that prevent harmful disease outcomes by modulating the responses of other leukocytes. In fact, we now demonstrate that bulk human blood NK cells c
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29

Cardarelli, Mariateresa, Youssef Rouphael, Marios C. Kyriacou, Giuseppe Colla, and Catello Pane. "Augmenting the Sustainability of Vegetable Cropping Systems by Configuring Rootstock-Dependent Rhizomicrobiomes that Support Plant Protection." Agronomy 10, no. 8 (2020): 1185. http://dx.doi.org/10.3390/agronomy10081185.

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Herbaceous grafting is a propagation method largely used in solanaceous and cucurbit crops for enhancing their agronomic performances especially under (a)biotic stress conditions. Besides these grafting-mediated benefits, recent advances about microbial networking in the soil/root interface, indicated further grafting potentialities to act as soil environment conditioner by modulating microbial communities in the rhizosphere. By selecting a suitable rootstock, grafting can modify the way of interacting root system with the soil environment regulating the plant ecological functions able to mode
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30

Tian, Ling, Rongsheng E. Wang, Ying Fei, and Yie-Hwa Chang. "A Homogeneous Fluorescent Assay for cAMP-Phosphodiesterase Enzyme Activity." Journal of Biomolecular Screening 17, no. 3 (2011): 409–14. http://dx.doi.org/10.1177/1087057111426901.

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Cyclic adenosine monophosphate–phosphodiesterases (cAMP-PDEs) regulate the cellular level of cAMP by selectively catalyzing the hydrolysis of the phosphodiester bond in the cAMP molecule. They play important roles in modulating cellular and physiological functions. There is a growing interest in the study of cAMP-PDEs as therapeutic targets. We describe a novel method for measuring the enzyme activity of cAMP-PDEs that is based on a homogeneous fluorescence assay employing a cAMP-dependent DNA-binding protein (CAP). We demonstrate that the assay is quick and robust compared to traditional meth
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31

Deng, Meihong, Li Xu, Rosemary Hoffmann, and Timothy R. Billiar. "TLR9 regulates host defense via modulation of peritoneal B1 cell recruitment and functions during intra-abdominal sepsis." Journal of Immunology 200, no. 1_Supplement (2018): 114.8. http://dx.doi.org/10.4049/jimmunol.200.supp.114.8.

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Abstract Background Toll like receptor 9 (TLR9) is identified as anintracellular DNA receptor playing important roles in immunity. However, the mechanisms of how TLR9 regulates host defense are unclear. B1 cells are aninnate-like B cell population predominately residing in peritoneum and highly express TLR9. Therefore, we hypothesize that TLR9 may regulate host defense via modulation of peritoneal B1 pathobiology during sepsis. Method C57BL/6 (WT) mice and TLR9−/− mice were subjected to cecal ligation and puncture (CLP) with for 18 hours. Result Deletion of TLR9 increased survival as well as d
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32

Mihaylenko, V., J. Chunyak, and V. Bachinskiy. "Analysis of processes in converter with eleven zone regulation output voltage." POWER ENGINEERING: economics, technique, ecology, no. 3 (April 6, 2020): 35–40. http://dx.doi.org/10.20535/1813-5420.3.2020.228610.

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 Analysis of the electromagnetic processes is organized beside this article in electric circuit with semiconductor commutator . Mathematical model is created for analysis electro-magnetic processes in semiconductor converter with width pulsed regulation of the output voltage. The broughted graphs, which reflect the electromagnetic processes in electric circuit. Method much parametric functions was used when performing calculation. The mathematical model of the converter is created for eleven zoned regulations of the output voltage. Article is devoted to the development of a
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33

Xia, Shaofeng, and Chenliang Wang. "Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1." International Journal of Genomics 2023 (June 16, 2023): 1–12. http://dx.doi.org/10.1155/2023/8845152.

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Circular RNAs (circRNAs) have been shown to have critical roles in developing cancer and treatment resistance in an increasing body of research. The aim was to look into the functions and processes of hsa_circ_0003220 in the non-small cell lung cancer (NSCLC) chemoresistance. The NSCLC cell lines H460 and A549 were employed in present work. hsa_circ_0003220, miR-489-3p, and insulin-like growth factors (IGF1) mRNA levels were assessed with a quantitative real time polymerase chain reaction (qRT-PCR). The cisplatin, docetaxel, and paclitaxel (PTX) resistances were determined using 3-(4,5-dimethy
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34

Popova, TA, II Prokofiev, IS Mokrousov, et al. "Effects of Neuroactive Amino Acids Derivatives on Cardiac and Cerebral Mitochondria and Endothelial functions in Animals Exposed to Stress." Journal of Clinical and Health Sciences 2, no. 2 (2017): 34. http://dx.doi.org/10.24191/jchs.v2i2.5885.

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Introduction: To study the effects of glufimet, a new derivative of glutamic acid, and phenibut, a derivative of γ-aminobutyric acid (GABA), on cardiac and cerebral mitochondria and endothelial functions in animals following exposure to stress and inducible nitric oxide synthase (iNOS) inhibition. Methods: Rats suspended by their dorsal cervical skin fold for 24 hours served as the immobilization and pain stress model. Arterial blood pressure was determined using a non-invasive blood pressure monitor. Mitochondrial fraction of heart and brain homogenates were isolated by differential centrifug
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35

Tabansky, Inna, Yupu Liang, Maya Frankfurt, et al. "Molecular profiling of reticular gigantocellularis neurons indicates that eNOS modulates environmentally dependent levels of arousal." Proceedings of the National Academy of Sciences 115, no. 29 (2018): E6900—E6909. http://dx.doi.org/10.1073/pnas.1806123115.

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Neurons of the medullary reticular nucleus gigantocellularis (NGC) and their targets have recently been a focus of research on mechanisms supporting generalized CNS arousal (GA) required for proper cognitive functions. Using the retro-TRAP method, we characterized transcripts enriched in NGC neurons which have projections to the thalamus. The unique expression and activation of the endothelial nitric oxide (eNOS) signaling pathway in these cells and their intimate connections with blood vessels indicate that these neurons exert direct neurovascular coupling. Production of nitric oxide (NO) wit
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36

Tsukanov, Vladislav V., Julia L. Tonkikh, Edward V. Kasparov та Alexander V. Vasyutin. "Inhibition of M2 tumor-associated macrophages polarization by modulating the Wnt/β-catenin pathway as a possible liver cancer therapy method". World Journal of Gastroenterology 30, № 40 (2024): 4399–403. http://dx.doi.org/10.3748/wjg.v30.i40.4399.

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The problem of liver cancer is becoming increasingly important due to the epidemic of metabolic diseases and persistent high alcohol consumption. This determines great attention to the development and improvement of methods for early diagnosis and treatment of liver cancer. Huang et al presented a study in the World Journal of Gastroenterology , in which they showed that the use of the traditional Chinese medicine Calculus bovis (CB) can suppress tumor growth in mice by inhibiting M2 tumor-associated macrophages (TAM) through modulating the activity of the Wnt/β-catenin pathway. The interactio
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37

Мihaylenko, V., J. Chunyak, K. Trubitsin, and V. Bachynskiy. "MATHEMATICAL MODEL OF A TWELVE-PULSE CONVERTER WITH SIXTEEN ZONE CONTROL OF THE OUTPUT VOLTAGE AND ACTIVE-INDUCTIVE LOADS." Collection of scholarly papers of Dniprovsk State Technical University (Technical Sciences) 2, no. 37 (2021): 68–72. http://dx.doi.org/10.31319/2519-2884.37.2020.13.

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Analysis of the electromagnetic processes is organized in this article in electric circuit with semiconductor commutator. Mathematical model twelve pulses of the converter is created for analysis of the electromagnetic processes in semiconductor converter with width-pulse regulation of the output voltage. The broughted graphs, which reflect the electromagnetic processes in electric circuit. Method multivariable function was used when performing calculation. The mathematical model of the converter is created for five zoned regulations of the output voltage. Using method multivariable function w
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38

Matyash, I. Ye, I. A. Minailova, O. M. Mischuk, and B. K. Serdega. "Component Analysis of Radiation-Induced Thermoe-lasticity Using Modulation Polarimetry." Ukrainian Journal of Physics 63, no. 11 (2018): 994. http://dx.doi.org/10.15407/ujpe63.11.994.

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A radiation field of an external or internal origin creates a non-uniform temperature gradient in a glass specimen. In this case, there appears a heat flux in the specimen, which generates mechanical stresses and induces an optical anisotropy in the form of birefringence. In this work, using the optical-polarization method, the birefringence magnitude is measured as the phase difference between the orthogonal components of the linearly polarized probing radiation. The capability of the method is enhanced by modulating the radiation polarization, which provided a reliable registration of stress
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39

Tada, Yuko, and Phillip C. Yang. "Iron Oxide Labeling and Tracking of Extracellular Vesicles." Magnetochemistry 5, no. 4 (2019): 60. http://dx.doi.org/10.3390/magnetochemistry5040060.

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Extracellular vesicles (EVs) are essential tools for conveying biological information and modulating functions of recipient cells. Implantation of isolated or modulated EVs can be innovative therapeutics for various diseases. Furthermore, EVs could be a biocompatible drug delivery vehicle to carry both endogenous and exogenous biologics. Tracking EVs should play essential roles in understanding the functions of EVs and advancing EV therapeutics. EVs have the characteristic structures consisting of the lipid bilayer and specific membrane proteins, through which they can be labeled efficiently.
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40

Peng, Xin, Zahid Khan, Yanan Dong, and Lian-Xi Xing. "Oral Transfer of Anti-Aging Substances: Key Chemical Found in Reproductive Caste of Termites." International Journal of Molecular Sciences 26, no. 4 (2025): 1543. https://doi.org/10.3390/ijms26041543.

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With the rapid increase in global population aging, the incidence and mortality rates of age-related diseases are rising, becoming a worldwide issue. Therefore, researching and discovering natural compounds with anti-aging properties is crucial. Social insects such as termites exhibit significant differences in lifespan between reproductive and non-reproductive castes. Reproductive castes are exclusively fed by worker termites through trophallaxis, providing a convenient model for the discovery of natural anti-aging compounds. This thesis systematically investigates the trophallactic fluid amo
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Gong, Xianghui, Xixi Zhao, Bin Li, et al. "Quantitative Studies of Endothelial Cell Fibronectin and Filamentous Actin (F-Actin) Coalignment in Response to Shear Stress." Microscopy and Microanalysis 23, no. 5 (2017): 1013–23. http://dx.doi.org/10.1017/s1431927617012454.

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AbstractBoth fibronectin (FN) and filamentous actin (F-actin) fibers play a critical role for endothelial cells (ECs) in responding to shear stress and modulating cell alignment and functions. FN is dynamically coupled to the F-actin cytoskeleton via focal adhesions. However, it is unclear how ECs cooperatively remodel their subcellular FN matrix and intracellular F-actin cytoskeleton in response to shear stress. Current studies are hampered by the lack of a reliable and sensitive quantification method of FN orientation. In this study, we developed a MATLAB-based feature enhancement method to
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42

Yeo, In-Seok, Yeo-Jin Yoon, Nari Seo, Hyun Joo An, and Jae-Han Kim. "Biopurification of Oligosaccharides by Immobilized Kluyveromyces Lactis." Applied Sciences 9, no. 14 (2019): 2845. http://dx.doi.org/10.3390/app9142845.

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Oligosaccharides with diverse and complex structures such as milk oligosaccharides have physiological functions including modulating intestinal microbiota or stimulating immune cell responses. However, milk carbohydrates include about 40–50% of lactose which requires a cost-effective method to separate. We developed a new method to purify the oligosaccharides from carbohydrate mixtures such as human milk oligosaccharides (HMOs) and galactooligosaccharides (GOSs) by exploiting immobilized Kluyveromyces lactis as microbial catalysts. Evaluation of media components exhibited no significant differ
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Lv, Meixia, Hongzhi Hu, Abuduheni Adila, Yibo Yan, Yang Liu, and Zunqi Liu. "Tunability of Photovoltaic Functions via Halogen Substitution [(Ade)2 CdX4](X = Cl, Br): A Class of Three-Dimensional Organic–Inorganic Hybrid Materials." Molecules 29, no. 12 (2024): 2773. http://dx.doi.org/10.3390/molecules29122773.

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Two new three-dimensional organic–inorganic hybrid crystalline materials, [(Ade)2 CdCl4] (1) and [(Ade)2 CdBr4] (2), were obtained by the slow evaporation of adenine (Ade) and cadmium chloride in aqueous solution at room temperature with hydrochloric acid and hydrobromic acid used as halogen sources. The structural, thermal, optical, and electrical properties were characterized by single-crystal X-ray diffraction, infrared spectroscopy, thermogravimetric analysis, variable-temperature–variable-frequency dielectric constant analysis, and electrochemical tests. With increasing the substitution o
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Meng, Fanhui, Jun Li, Wenjun Wang, and Yan Fu. "Gengnianchun, a Traditional Chinese Medicine, Enhances Oxidative Stress Resistance and Lifespan in Caenorhabditis elegans by Modulating daf-16/FOXO." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/8432306.

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Objective. Gengnianchun (GNC), a traditional Chinese medicine (TCM), is primarily used to improve declining functions related to aging. In this study, we investigated its prolongevity and stress resistance properties and explored the associated regulatory mechanism using a Caenorhabditis elegans model. Methods. Wild-type C. elegans N2 was used for lifespan analysis and oxidative stress resistance assays. Transgenic animals were used to investigate pathways associated with antioxidative stress activity. The effects of GNC on levels of reactive oxygen species (ROS) and expression of specific gen
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Chen, Xiao, Alex Morehead, Jian Liu, and Jianlin Cheng. "A gated graph transformer for protein complex structure quality assessment and its performance in CASP15." Bioinformatics 39, Supplement_1 (2023): i308—i317. http://dx.doi.org/10.1093/bioinformatics/btad203.

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Abstract Motivation Proteins interact to form complexes to carry out essential biological functions. Computational methods such as AlphaFold-multimer have been developed to predict the quaternary structures of protein complexes. An important yet largely unsolved challenge in protein complex structure prediction is to accurately estimate the quality of predicted protein complex structures without any knowledge of the corresponding native structures. Such estimations can then be used to select high-quality predicted complex structures to facilitate biomedical research such as protein function an
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Pang, Tianxiang, Qinghua Li, Jianxiang Wang, Wensu Yuan, Lei Zhang, and Zhongchao Han. "Effect of Roline Rich Domain of an RNA-Binding Protein Sam68 in B Lymphocyte Leukemia Cell Line Growth Process, Death and B Cell Signal Transduction." Blood 108, no. 11 (2006): 4465. http://dx.doi.org/10.1182/blood.v108.11.4465.4465.

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Abstract Sam68 plays an important role as a multiple functional RNA binding nuclear protein in cell cycle progress, RNA usage, signal transduction regulation of cell growth and proliferation, and tyrosine phosphorylation by Src during mitosis. However, its precise impact on these essential cellular functions remains unclear. The purpose of this study is to further elucidate Sam68 functions in B lymphocyte leukemia DT40 cell line. By using gene targeting method, we isolated a mutation of deleted proline rich domain from Sam68 in DT40 cells and described its effect on cell growth process and sig
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Sun, Eryi, and Ping Zhang. "RNF12 Promotes Glioblastoma Malignant Proliferation via Destructing RB1 and Regulating MAPK Pathway." Journal of Healthcare Engineering 2021 (November 30, 2021): 1–8. http://dx.doi.org/10.1155/2021/4711232.

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Background. RNF12 has been linked to a variety of biological activities, including the control of the MDM2/P53 pathway, although its additional functions remain unclear. RNF12 was discovered to be a new ubiquitin ligase (E3) for RB1, amongst the most frequently repressed proteins in cancer of human. Method. Cell Counting Kit-8 was used to detect the cell proliferation; coimmunoprecipitation was used to determine that RNF12 interacts with RB1. Xenograft studies were used to verify the results. Result. In vivo and in vitro RNF12 interacts with RB1 regardless of E3 ligase activity. The ubiquitina
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Bhatt, Megha, Prafull Salvi, and Pushpa Lohani. "Gateway cloning and in-planta transformation of drought stress responsive Ecmyb1 gene isolated from Eleusine coracana var.PRM 6107." Environment Conservation Journal 22, no. 1&2 (2021): 205–11. http://dx.doi.org/10.36953/ecj.2021.221229.

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Drought is one of the key abiotic stress that critically influences the crops by restraining their growth and yield potential. Being sessile, plants tackle the detrimental effects of drought stress via modulating the cellular state by changing the gene expression. Such alteration of gene expression is essentially driven by the transcriptional syndicate. Transcription factors (TF) are the key regulatory protein that controls the expression of their target gene by binding to the cis-regulatory elements present in the promoter region. Myb-TFs ubiquitously present in all eukaryotes belong to one o
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Giddens, John P., Joseph V. Lomino, David J. DiLillo, Jeffrey V. Ravetch, and Lai-Xi Wang. "Site-selective chemoenzymatic glycoengineering of Fab and Fc glycans of a therapeutic antibody." Proceedings of the National Academy of Sciences 115, no. 47 (2018): 12023–27. http://dx.doi.org/10.1073/pnas.1812833115.

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The N-glycans attached to the Fab and Fc domains play distinct roles in modulating the functions of antibodies. However, posttranslational site-selective modifications of glycans in antibodies and other multiply glycosylated proteins remain a challenging task. Here, we report a chemoenzymatic method that permits independent manipulation of the Fab and Fc N-glycans, using cetuximab as a model therapeutic monoclonal antibody. Taking advantage of the substrate specificity of three endoglycosidases (Endo-S, Endo-S2, and Endo-F3) and their glycosynthase mutants, together with an unexpected substrat
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Benigni, Valentina. "È stato un mezzo disastro… Fenomeni di convergenza tra approssimazione e intensificazione: uno studio contrastivo su italiano e russo." Italica Wratislaviensia 14, no. 1 (2023): 9–34. http://dx.doi.org/10.15804/iw.2023.14.1.01.

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Based on the case-study of the Italian downtoner mezzo, ‘half of’, the present work aims to highlight, through a usage-based approach, interesting points of semantic and functional convergence between approximation and intensification, which are traditionally considered divergent meaningconstruction strategies. Mezzo functions both on the semantic level, by modulating the referential force of nouns, verbs, and adjectives, and on the pragmatic level, where it acts as a discourse marker, enabling the speaker to reduce the illocutionary force of their assertions and helping the receiver to make t
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