Relevant bibliographies by topics / Molecular cloning, recombinant DNA technology

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Academic literature on the topic 'Molecular cloning, recombinant DNA technology'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 February 2022

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Journal articles on the topic "Molecular cloning, recombinant DNA technology"

Dulal, Kalpana, Benjamin Silver, and Hua Zhu. "Use of Recombination-Mediated Genetic Engineering for Construction of Rescue Human Cytomegalovirus Bacterial Artificial Chromosome Clones." Journal of Biomedicine and Biotechnology 2012 (2012): 1–10. http://dx.doi.org/10.1155/2012/357147.

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Bacterial artificial chromosome (BAC) technology has contributed immensely to manipulation of larger genomes in many organisms including large DNA viruses like human cytomegalovirus (HCMV). The HCMV BAC clone propagated and maintained insideE. coliallows for accurate recombinant virus generation. Using this system, we have generated a panel of HCMV deletion mutants and their rescue clones. In this paper, we describe the construction of HCMV BAC mutants using a homologous recombination system. A gene capture method, or gap repair cloning, to seize large fragments of DNA from the virus BAC in or

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Murray, Noreen E. "The impact of phage lambda: from restriction to recombineering." Biochemical Society Transactions 34, no. 2 (2006): 203–7. http://dx.doi.org/10.1042/bst0340203.

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Experiments using phage lambda provided early insights into important molecular mechanisms, including genetic recombination and the control of gene expression. Before recombinant DNA technology, the use of lambda, most particularly lambda transducing phages, illustrated the importance of cloning bacterial genes, already providing some insight into how to use cloned genes to advantage. Subsequently, lambda made significant contributions to recombinant DNA technology, including the early generation of genomic and cDNA libraries. More recently, lambda genes associated with recombination have enab

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D., Batsaikhan, Lkhagvasuren S., Munkhtogtokh B., Tuvshin B., Bayar-Enkh B., and Ganbold Ya. "AN ATTEMPT TO PRODUCE RECOMBINANT HORSE INSULIN." Mongolian Journal of Agricultural Sciences 19, no. 3 (2017): 3–9. http://dx.doi.org/10.5564/mjas.v19i3.728.

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The present study aimed to produce horse insulin by use of recombinant DNA technology. Because the hormones such as somatotropin, prolactin and leptin, which are practically applicable in veterinary medicine, are also peptides, they can be obtained on the basis of this model technology. To isolate total RNA, horse pancreas samples were collected in RNA stabilizing solution from local abattoirs, sent to the laboratory, and stored the samples in the freezer at -80°C. Molecular biological reagents and kits for total RNA extraction,one step RT (reverse transcription)-PCR reactions, cloning, expres

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Hobden, A. N., and T. J. R. Harris. "The impact of biotechnology and molecular biology on the pharmaceutical industry." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 99, no. 1-2 (1992): 37–45. http://dx.doi.org/10.1017/s0269727000013038.

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Synopsis:Biotechnology had its initial impact on the pharmaceutical industry well before the perceived time. The use of fermentation technology to produce antibiotics was a cornerstone for the development of the industry. This event was both before cloning (BC) and before DNA (rather than after DNA – AD). Even now the antibiotic market, which is worth over 10 billion U.S. dollars a year, is the most valuable segment of the total market, (c.200 billion dollars per year). Nevertheless the impact of biotechnology in drug discovery was until recently perceived solely to be the use of recombinant D

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Fakruddin, Md, Reaz Mohammad Mazumdar, Khanjada Shahnewaj Bin Mannan, Abhijit Chowdhury, and Md Nur Hossain. "Critical Factors Affecting the Success of Cloning, Expression, and Mass Production of Enzymes by Recombinant E. coli." ISRN Biotechnology 2013 (September 13, 2013): 1–7. http://dx.doi.org/10.5402/2013/590587.

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E. coli is the most frequently used host for production of enzymes and other proteins by recombinant DNA technology. E. coli is preferable for its relative simplicity, inexpensive and fast high-density cultivation, well-known genetics, and large number of compatible molecular tools available. Despite all these advantages, expression and production of recombinant enzymes are not always successful and often result in insoluble and nonfunctional proteins. There are many factors that affect the success of cloning, expression, and mass production of enzymes by recombinant E. coli. In this paper, th

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Tami, Joseph A. "Major Techniques of Biotechnology." Journal of Pharmacy Practice 11, no. 1 (1998): 28–37. http://dx.doi.org/10.1177/089719009801100106.

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Since the discovery of the structure and function of DNA over 40 years ago, the established knowledge of molecular biology has increased dramatically, and many new tools have been discovered and utilized by scientists to develop new therapeutic agents. Important tools that are used in recombinant DNA technology include restriction endonucleases (cleave DNA), DNA ligase (link DNA molecules together), and cloning vectors (place foreign DNA into an organism such as bacterial or yeast cells in order to mass produce the protein encoded by that foreign DNA). The development of hybridoma technology p

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Pradhananga, Sarbendra, and Jon R. Sayers. "Natural synthesis: Biologics, biosimilars and biobetters in protein hormone therapy." Biochemist 34, no. 1 (2012): 10–15. http://dx.doi.org/10.1042/bio03401010.

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Hormone therapies have been used since the early 20th Century and belong to a group of drugs that has recently become known as ‘biologics’. Biologics are medicinal products that have been produced by biological processes as opposed to chemically synthesized drugs. The term biologics spans a wide range of products that include therapeutics such as organs, tissue, cells, blood or blood components, vaccines and proteins. This ‘proteins’ subgroup can be further subdivided into therapeutics such as antibodies, enzymes and hormones. The first hormone therapeutics were extracted from human or animal

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Klein, Ronald D., and Timothy G. Geary. "Recombinant Microorganisms as Tools for High Throughput Screening for Nonantibiotic Compounds." Journal of Biomolecular Screening 2, no. 1 (1997): 41–49. http://dx.doi.org/10.1177/108705719700200108.

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Microorganisms were among the first tools used for the discovery of biologically active compounds. Their utility reached a zenith during the era of antibiotic development in the 1950s and 1960s, then declined. Subsequently, a substantial role for microorganisms in the pharmaceutical industry developed with the realization that microbial fermentations were intriguing sources of nonantibiotic natural products. From recombinant DNA technology emerged another important role for microorganisms in pharmaceutical research: the expression of heterologous proteins for therapeutic products or for in vit

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Lan Anh, Le Thi, Minh Thi Hang, Nguyen Thi Thu Hien, et al. "Cloning, expression and purification of Leptospira LigB antigen in Escherichia coli." Vietnam Journal of Biotechnology 17, no. 3 (2020): 569–75. http://dx.doi.org/10.15625/1811-4989/17/3/14364.

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Leptospira is one of the most common zoonotic diseases in the tropics and subtropics. Humans are infected by exposure to Leptospira contained water or food sources. Leptospirosis usually breaks out after the flood and causes several consequences for people and economy. Leptospirosis disease, if not rapidly detected and treated promptly, it causes serious consequences such as acute hepatitis-kidneys, meningitis and bleeding, heart and nerve complications, and severe illness can lead to death. Therefore, quick and accurate detection of Leptospira pathogen plays a very important role in Leptospir

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Ausubel, Frederick M. "Tracing My Roots: How I Became a Plant Biologist." Annual Review of Genetics 52, no. 1 (2018): 1–20. http://dx.doi.org/10.1146/annurev-genet-120417-031722.

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My trajectory to becoming a plant biologist was shaped by a complex mix of scientific, political, sociological, and personal factors. I was trained as a microbiologist and molecular biologist in the late 1960s and early 1970s, a time of political upheaval surrounding the Vietnam War. My political activism taught me to be wary of the potential misuses of scientific knowledge and to promote the positive applications of science for the benefit of society. I chose agricultural science for my postdoctoral work. Because I was not trained as a plant biologist, I devised a postdoctoral project that to

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Dissertations / Theses on the topic "Molecular cloning, recombinant DNA technology"

Vedoveli, Naiara Cristina Pulzi Saito. "Construção e análise funcional de vetores lentivirais de interesse biotecnológico." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17156/tde-26082016-152814/.

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Vetores lentivirais são ferramentas fundamentais para modificação celular. Sua utilização ganhou destaque devido à capacidade desses em integrar ao genoma de células que estão ou não em divisão. Grande parte dos vetores desenvolvidos são derivados do genoma do Vírus da Imunodeficiência Humana (HIV-1), portanto, modificações foram necessárias a fim de evitar a formação de Partículas Competentes em Replicação (RCLs) e garantir uma utilização segura. Com as modificações, foram produzidos os vetores lentivirais de terceira geração utilizados atualmente. Esses vetores podem ser usados para expressã

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Loftus, M. G. "The application of recombinant DNA technology to the cultivated mushroom, Agaricus bisporus." Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383176.

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Pardigon, Nathalie. "Le clonage moleculaire du segment m du bunyavirus germiston : etude de la transcription et de la traduction a l'aide de cadn recombinants." Paris 7, 1988. http://www.theses.fr/1988PA077134.

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"Molecular cloning and protein characterization of the developmentally regulated human 1433 epsilon isoform." 1997. http://library.cuhk.edu.hk/record=b6073070.

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by Sharon, Chui-Wah Luk.<br>Thesis (Ph.D.)--Chinese University of Hong Kong, 1997.<br>Includes bibliographical references (p. 128-146).<br>Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.<br>Mode of access: World Wide Web.

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Books on the topic "Molecular cloning, recombinant DNA technology"

Dale, Jeremy. From genes to genomes: Concepts and applications of DNA technology. 2nd ed. John Wiley & Sons, 2007.

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Winnacker, Ernst L. From genes to clones: Introduction to gene technology. VCH, 1987.

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von, Schantz Malcolm, and Plant Nick, eds. From genes to genomes: Concepts and applications of DNA technology. 3rd ed. John Wiley & Sons, 2011.

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A practical guide to molecular cloning. 2nd ed. Wiley, 1988.

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Gene cloning and manipulation. Cambridge University Press, 1995.

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Viktorovich, Vlasov Valentin, ed. Klonirovanie genov. Izd-vo "Nauka," Sibirskoe otd-nie, 1986.

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Bothwell, Alfred. Methodsfor cloning and analysis of eukaryotic genes. Jones and Bartlett, 1990.

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Gene cloning and DNA analysis: An introduction. 6th ed. Wiley-Blackwell, 2010.

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Understanding DNA and gene cloning: A guide for the curious. 3rd ed. Wiley, 1997.

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Understanding DNA and gene cloning: A guide for the curious. 2nd ed. Wiley, 1992.

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Book chapters on the topic "Molecular cloning, recombinant DNA technology"

Smith-Keary, Peter. "Recombinant DNA technology." In Molecular Genetics. Macmillan Education UK, 1991. http://dx.doi.org/10.1007/978-1-349-11732-1_13.

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Smith, C. A., and E. J. Wood. "Recombinant DNA technology." In Molecular Biology and Biotechnology. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3866-0_9.

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Ross, Dennis W. "Tools of Recombinant DNA Technology." In Introduction to Molecular Medicine. Springer New York, 1996. http://dx.doi.org/10.1007/978-1-4757-2460-8_3.

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Ross, Dennis W. "Tools of Recombinant DNA Technology." In Introduction to Molecular Medicine. Springer New York, 1992. http://dx.doi.org/10.1007/978-1-4757-4076-9_3.

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Priyadarshini, Anjali, and Prerna Pandey. "Genetic Manipulation by Recombinant DNA Technology." In Molecular Biology. Apple Academic Press, 2018. http://dx.doi.org/10.1201/b22354-5.

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Temin, Howard M. "Developments in Molecular Virology: Cloning of Retrovirus DNA in Bacteria and Cloning of other DNA in Retroviruses." In Recombinant DNA Research and Viruses. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2565-9_1.

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Stoltzfus, Jon R. "Enzymes and Cloning Vectors Used to Create Recombinant DNA, Characteristics of." In Molecular Life Sciences. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4614-1531-2_84.

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Stoltzfus, Jon R. "Characteristics of Enzymes and Cloning Vectors Used to Create Recombinant DNA." In Molecular Life Sciences. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-6436-5_84-2.

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Gottschamel, Johanna, and Andreas Lössl. "Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3289-4_1.

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Benz, E. J. "Introduction to Molecular Genetics and Recombinant DNA Technology." In Biotechnology in blood transfusion. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1761-6_2.

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Conference papers on the topic "Molecular cloning, recombinant DNA technology"

Uzan, G., A. Lajmanovich, M. H. Prandini, Ph Frachet, A. Duperray, and G. Marguerie. "MOLECULAR CLONING OF PLATELET GPIIb FROM HEL CELLS AND HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643960.

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Platelet GP IIb-IIIa is an heterodimer which functions as a receptor for fibrinogen, fibronectin and Von Willebrand factor and is implicated in platelet adhesive reactions. To study the structure function relationship of this glycoprotein, a recombinant DNA approach was initiated. cDNA expression libraries were constructed in » gtll vector, from erythro-leukemia cells (HEL) and megakaryocytes mRNA. The human megakaryocytes were isolated from patients with chronic myeloid leukemia. The HEL library was initially screened with polyclonal antibodies anti GPIIb IIIa. One clone, λIIbI, containing a

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Liu, Xiaohong. "Notice of Retraction: A Report on the Teaching Contents and Methods of DNA Recombinant Technology in the Course Molecular Biology." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780096.

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Ny, T., L. Hansson, and B. Åstedt. "ISOLATION OF cDNA FOR TYPE-2 PLASMINOGEN ACTIVATOR INHIBITOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642855.

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The placental type plasminogen activator inhibitor (PAI-2) has been purified from extracts of human placenta and from a histiocytic lymphoma cell line. It is mainly an uPA inhibitor but it also inhibits the two-chain form of tPA.In order to determine the factors regulating PAI-2 gene expression and thereby clarify the physiological role of PAI-2 we have undertaken the molecular cloning of PAI-2 cDNA. A λgt11 expression library prepared from placental mRNA, was screened, immunologically using a monoclonal antibody probe developed against PAI-2 purified from human placenta. When 1.7×105 recombin

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Freund, M., J.-P. Cazenave, M.-L. Wiesel, et al. "RECOMBINANT HIRUDIN INHIBITS EXPERIMENTAL VENOUS THROMBOSIS INDUCED BY INJECTION OF TISSUE FACTOR AND STASIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643917.

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Hirudin (HIR), a polypeptide of 65 aminoacids, is the most potent natural inhibitor of coagulation by forming rapidly a very stable and specific non covalent 1:1 complex with α-thrombin, independent of antithrombin III. Although natural HIR has in vivo anticoagulant and antithrombotic properties, its limited availability for large scale purification has prevented further clinical testing and potential use; this can now be solved by recombinant DNA technology. We have previously reported the cloning and expression of a cDNA encoding one variant (called HV-2) of Hirudo medicinalis HIR (Proc. Nat

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