Academic literature on the topic 'Molecular cloning techniques'

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Journal articles on the topic "Molecular cloning techniques"

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Malcolm, S. "Guide to Molecular Cloning Techniques." Journal of Medical Genetics 27, no. 1 (1990): 70. http://dx.doi.org/10.1136/jmg.27.1.70.

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Darlison, Mark G. "Guide to molecular cloning techniques." Trends in Neurosciences 11, no. 11 (1988): 510–11. http://dx.doi.org/10.1016/0166-2236(88)90014-8.

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Dong, Xu, Yi Xin, Li Ye, and Yufang Ma. "UsingPseudomonas putida xylEgene to teach molecular cloning techniques for undergraduates." Biochemistry and Molecular Biology Education 37, no. 6 (2009): 339–43. http://dx.doi.org/10.1002/bmb.20328.

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Karn, Jonathan. "Methods in enzymology, vol. 152: guide to molecular cloning techniques." Trends in Genetics 4, no. 9 (1988): 268. http://dx.doi.org/10.1016/0168-9525(88)90035-2.

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Perrett, C. W. "Analysis of brain gene expression in schizophrenia by molecular cloning techniques." Schizophrenia Research 2, no. 1-2 (1989): 46. http://dx.doi.org/10.1016/0920-9964(89)90082-0.

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Roberts, Robert. "Impact for molecular biology in cardiology." American Journal of Physiology-Lung Cellular and Molecular Physiology 261, no. 4 (1991): L8—L14. http://dx.doi.org/10.1152/ajplung.1991.261.4.l8.

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The recent development and application of the techniques of recombinant DNA and molecular biology ignited an explosion in biomedical research, which has been embraced by medicine. However, cardiology as a subspecialty has been slower in adopting these techniques, in part because the heart is a nonproliferating organ and in part because it was not easily accessible until recently. The techniques of recombinant DNA were not possible until the 1970s. In that decade four major discoveries occurred that launched molecular biology into the 21st century. These seminal contributions were 1) the discov
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Roberts, Robert. "Impact for molecular biology in cardiology." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 4 (1991): 8–14. http://dx.doi.org/10.1152/ajpheart.1991.261.4.8.

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The recent development and application of the techniques of recombinant DNA and molecular biology ignited an explosion in biomedical research, which has been embraced by medicine. However, cardiology as a subspecialty has been slower in adopting these techniques, in part because the heart is a nonproliferating organ and in part because it was not easily accessible until recently. The techniques of recombinant DNA were not possible until the 1970s. In that decade four major discoveries occurred that launched molecular biology into the 21st century. These seminal contributions were 1) the discov
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Tami, Joseph A. "Major Techniques of Biotechnology." Journal of Pharmacy Practice 11, no. 1 (1998): 28–37. http://dx.doi.org/10.1177/089719009801100106.

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Since the discovery of the structure and function of DNA over 40 years ago, the established knowledge of molecular biology has increased dramatically, and many new tools have been discovered and utilized by scientists to develop new therapeutic agents. Important tools that are used in recombinant DNA technology include restriction endonucleases (cleave DNA), DNA ligase (link DNA molecules together), and cloning vectors (place foreign DNA into an organism such as bacterial or yeast cells in order to mass produce the protein encoded by that foreign DNA). The development of hybridoma technology p
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Silver, S. C., and S. W. Hunt. "Techniques for cloning cDNAs encoding interactive transcriptional regulatory proteins." Molecular Biology Reports 17, no. 3 (1993): 155–65. http://dx.doi.org/10.1007/bf00986725.

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Миненко, I. Minenko, Сердюков, and D. Serdyukov. "To The Question of the Cloninig History." Journal of New Medical Technologies. eJournal 8, no. 1 (2014): 1–9. http://dx.doi.org/10.12737/2907.

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Cloning in biology - cloning in biology is getting both asexually by natural means, including vegetative reproduction, and by means of biotechnological methods genetically identical organisms, cells or molecules. In the XXI century, in the conditions of the impetuous growth of the population and the food problem aggravation, humanity faces a growing and more demanding challenge of creating the necessary quantity of living organisms-plants and animals, with pre-defined properties: resistant to disease, climate fluctuations, high productivity, fertility, desired flavoring, aromatic, nourishing,
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Dissertations / Theses on the topic "Molecular cloning techniques"

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Fairbairn, L. J. "Investigations on erythrocyte membrane proteins using molecular cloning techniques." Thesis, University of Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379600.

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Woodward, Robert. "Molecular and immunological characterization of cytoskeletal proteins in Trypanosoma brucei." Thesis, University of Kent, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292369.

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Baker, N. E. "Wingless : A gene required for segmentation in Drosophila." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377244.

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O'Neill, Andrew. "The microbial diversity of wetland sediments constructed to treat acid mine drainage as determined by molecular techniques /." St. Lucia, Qld, 2001. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16527.pdf.

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High, S. "Studies of the human red cell sailoglycoproteins using the techniques of molecular cloning." Thesis, University of Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381052.

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Andorrà, Solsona Immaculada. "Adaptation and development of culture-independent techniques for the indentification and enumeration of microorganisms in wine fermentations." Doctoral thesis, Universitat Rovira i Virgili, 2010. http://hdl.handle.net/10803/8690.

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L'objectiu d'aquesta tesi va ser l'adaptació i validació de diferents tècniques independents de cultiu per a la detecció i quantificació de la microbiota present a la fermentació vínica. Es van assajar la QPCR (PCR quantitativa) per a la quantificació i seguiment d'espècies clau i la diversitat ecològica es va analitzar per DGGE (electroforesi en gel desnaturalitzant) i la clonació d'un fragment ribosomal.<br/>Tanmateix es va estudiar l'aplicació de la Hibridació in Situ (FISH) i la QPCR amb colorants específics per tal de diferenciar cèl·lules vives i mortes. <br/>Aquestes tècniques es varen
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Coccia, Marco Anthony. "Identification and cloning of amplified DNA sequences by a modified-in-gel renaturation technique: Isolation of an amplified DNA sequence from a human sarcoma." Diss., The University of Arizona, 1991. http://hdl.handle.net/10150/185368.

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A modified in gel renaturation technique has been developed which detects DNA sequence amplifications of ≥10-fold without prior identification of the target gene. In order to determine the sensitivity of detection and applicability of modified in gel renaturation, primary human tumor cultures and drug resistant human cell lines with identified cellular gene amplifications were assayed. The modified in gel renaturation technique was then used to screen 71 human tumor cell lines and direct tumor biopsies for amplified sequences. Particular emphasis was placed on screening cell lines derived from
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Yeh, Patrice. "Biosynthese de la lysine chez corynebacterium glutamicum : clonage et organisation des genes." Toulouse 3, 1988. http://www.theses.fr/1988TOU30089.

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Bruyère, Thierry. "Etude immunologique et genetique d'une adhesine de streptococcus mutans." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13044.

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Gowan, Bhavna Manilal. "Development of techniques for cloning Nocardioform genes of the enzymes involved in detoxifying acrylamide." Thesis, 2015. http://hdl.handle.net/10539/16693.

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Books on the topic "Molecular cloning techniques"

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R, Kimmel Alan, ed. Guide to molecular cloning techniques. Academic Press, 1987.

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François, Baneyx, ed. Introduction to molecular cloning techniques. VCH Publishers, 1993.

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A, Fuchs James, and Messing Joachim W, eds. An introduction to recombinant DNA techniques: Basic experiments in gene manipulation. 2nd ed. Benjamin/Cummings, 1988.

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1955-, Whitney Donald B., ed. Biotechnology: Proteins to PCR : a course in strategies and lab techniques. Birkhäuser, 1995.

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Introduction to Molecular Cloning Techniques. Wiley-VCH, 1993.

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Guide to molecular cloning techniques. Academic Press, 1987.

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Introduction to Molecular Cloning Techniques. Wiley-VCH Verlag GmbH, 1993.

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8

(Editor), Alan R. Kimmel, ed. Guide to Molecular Cloning Techniques, Volume 152: Volume 152: Guide to Molecular Cloning Techniques (Methods in Enzymology). Academic Press, 1987.

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Guide to Molecular Cloning Techniques, Volume 152: Volume 152: Guide to Molecular Cloning Techniques (Methods in Enzymology). Academic Press, 1987.

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(Editor), Shelby L. Berger, and Alan R. Kimmel (Editor), eds. Methods in Enzymology, Volume 152: Guide to Molecular Cloning Techniques. Academic Press, 1987.

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Book chapters on the topic "Molecular cloning techniques"

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Deichmann, Annette, and Klaus Deichmann. "Cloning Vectors." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_15.

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Gingold, Elliot B. "Cloning in Yeast." In Techniques in Molecular Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_8.

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Gaastra, Wim, and Hans E. N. Bergmans. "Plasmid Derived Cloning Vectors." In Techniques in Molecular Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_6.

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Dale, Jeremy W. "Cloning in Bacteriophage Lambda." In Techniques in Molecular Biology. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9799-5_9.

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Hildebrandt, Friedhelm. "Cloning Strategies: An Overview." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_16.

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Markovich, Daniel, Andreas Werner, and Heini Murer. "Expression Cloning with Xenopus Oocytes." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_20.

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Hildebrandt, Friedhelm, and Heymut Omran. "Positional Cloning and Linkage Analysis." In Techniques in Molecular Medicine. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59811-1_23.

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Surzycki, Stefan. "DNA Cloning. General Consideration." In Basic Techniques in Molecular Biology. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-56968-5_13.

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Surzycki, Stefan. "DNA Cloning - Experimental Procedures." In Basic Techniques in Molecular Biology. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-56968-5_14.

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Subbotin, Sergei A. "Molecular identification of nematodes using polymerase chain reaction (PCR)." In Techniques for work with plant and soil nematodes. CABI, 2021. http://dx.doi.org/10.1079/9781786391759.0218.

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Abstract Molecular diagnostics are a vital component of the management of economically important pests, including plant-parasitic nematodes. Various molecular techniques for diagnostics have been introduced to nematology during last decades, but the most popular is Polymerase Chain Reaction (PCR) based. This chapter presents procedures for DNA extraction, PCR techniques, cloning and DNA sequencing of nematodes.
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Conference papers on the topic "Molecular cloning techniques"

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Wicki, A. N., A. Walz, and K. J. Clemetson. "IDENTIFICATION OF GLYCOPROTEIN lb IN “IN VITRO” TRANSLATES FROM ISOLATED HUMAN PLATELET mRNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643628.

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Platelet membrane glycoproteins play a crucial role in platelet adhesion and activation. To understand how they function it is of great importance to know their amino acid sequences and structures. It is rather difficult to purify membrane glycoproteins in amounts that are sufficient to determine their amino acid sequences by protein sequencing techniques. The easier way seems to be molecular cloning of the genes for these proteins.Metabolically stable mRNA derived from nucleated megakaryocytes is known to be present in the anuclear human platelets. We have developed a purification method for
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Ergenc, Ali Fuat, and Nejat Olgac. "Optical Fiber Sensor for Non-Contact Monitoring of ICSI-Pipettes." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-13178.

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The injection of cells (e.g., sperm), subcellular organelles (e.g., nucleus), and solutes into cells (e.g., eggs) for cloning, embryology and molecular biology purposes are broadly practiced biological procedures. These cellular micro-injection operations are performed using drawn glass pipettes of microscopic diameters (as small as a few microns), which are extremely compliant structures. The pipette is pressed on the cell membrane causing a dimple until a predetermined tension is created. In the next phase, the membrane is pierced using various forcing techniques, and the pipette is driven i
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