Journal articles: 'Molecular markers ISSR'

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Ismail, Nor Asiah, M. Y. Rafii, T. M. M. Mahmud, M. M. Hanafi, and Gous Miah. "Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers." BioMed Research International 2019 (May 6, 2019): 1–14. http://dx.doi.org/10.1155/2019/5904804.

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Fifty-seven accessions of torch ginger (Etlingera elatior) collected from seven states in Peninsular Malaysia were evaluated for their molecular characteristics using ISSR and SSR markers to assess the pattern of genetic diversity and association among the characteristics. Diversity study through molecular characterization showed that high variability existed among the 57 torch ginger accessions. ISSR and SSR molecular markers revealed the presence of high genetic variability among the torch ginger accessions. The combination of different molecular markers offered reliable and convincing information about the genetic diversity of torch ginger germplasm. This study found that SSR marker was more informative compared to ISSR marker in determination of gene diversity, polymorphic information content (PIC), and heterozygosity in this population. SSR also revealed high ability in evaluating diversity levels, genetic structure, and relationships of torch ginger due to their codominance and rich allelic diversity. High level of genetic diversity discovered by SSR markers showed the effectiveness of this marker to detect the polymorphism in this germplasm collection.

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Monte-Corvo, Luisa, Luis Goulão, and Cristina Oliveira. "ISSR Analysis of Cultivars of Pear and Suitability of Molecular Markers for Clone Discrimination." Journal of the American Society for Horticultural Science 126, no. 5 (September 2001): 517–22. http://dx.doi.org/10.21273/jashs.126.5.517.

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Inter-simple sequence repeat (ISSR) markers were used for cultivar identification and for determination of the phenetic relationships among 24 pear cultivars (Pyrus communis L.). The ability of several molecular marker systems including randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLP), inter-simple sequence repeats (ISSR), simple sequence repeats (SSR), and selective amplification of microsatellite polymorphic loci (SAMPL) to detect variation among clones of the most significant Portuguese cultivar, Rocha, was also investigated. Each of the eight ISSR primers tested was able to distinguish the 24 pear cultivars. The ISSR primers generated 337 markers, 79.5% of which were polymorphic. The cultivar dendrogram obtained with the ISSR marker data was very similar to that obtained with previous RAPD+AFLP analysis, confirming the genetic divergence of `Pérola', `Carvalhal' and `Lawson' from the other cultivars. Eight out of 15 apple [Malus sylvestris (L.) Mill. var domestica (Borkh.) Mansf.] SSR primers tested also amplified microsatellites in pear. None of the five molecular marker systems analyzed (with a total of 1082 markers) detected reproducible polymorphisms among the nine `Rocha' clones, in spite of the presence of clear phenotypic differences.

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György, Zsuzsanna, Norbert Incze, and Zsuzsanna Pluhár. "Differentiating Thymus vulgaris chemotypes with ISSR molecular markers." Biochemical Systematics and Ecology 92 (October 2020): 104118. http://dx.doi.org/10.1016/j.bse.2020.104118.

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Mishra, Kumar, Sandhyarani Nishani, and J. Jayarama. "Molecular identification and genetic relationships among coffee species (Coffea L.) inferred from ISSR and SRAP marker analyses." Archives of Biological Sciences 63, no. 3 (2011): 667–79. http://dx.doi.org/10.2298/abs1103667m.

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The identification and genetic relationships of 23 coffee species and one coffee-related species Canthium diccocum were studied using ISSR and SRAP markers. The average polymorphism information content of SRAP primers (0.81) was lower than ISSR primers (0.86), whereas the average resolving power of the SRAP primers (9.74) is higher than the ISSR primers (8.64). The genetic similarity among the species ranged from 0.30 to 0.89 using ISSR and 0.11 to 0.90 using SRAP marker systems. Based on marker analysis, all twenty three coffee species were clustered into two major groups. Both the markers amplified species-specific fragments and are useful in genetic diversity analysis of coffee.

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Boyd, Meagan, Mary Anne Panoyan, Paul Michael, and Kabwe K. Nkongolo. "Development and characterization of species-diagnostic ISSR and SCAR DNA markers for differentiating red maple (Acer rubrum) and silver maple (A. saccharinum)." Genome 62, no. 8 (August 2019): 527–35. http://dx.doi.org/10.1139/gen-2019-0037.

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Red maple (Acer rubrum) and silver maple (A. saccharinum) are sister species that readily hybridize in nature. No genetic or barcoding markers have been tested in these species. The main objective of the present study is to develop and characterize molecular markers for distinguishing A. rubrum and A. saccharinum and to validate the hybridity of A. freemanii derived from their crossings using the ISSR marker system. Thirteen A. rubrum and seven A. saccharinum populations were used. Four ISSR primers including ISSR 5, ISSR 8, ISSR 10, and ISSR UBC 825 were selected to amplify genomic DNA from the two species and their hybrids. Each primer generated at least one species-diagnostic ISSR marker for a total of six. Analysis of A. freemanii collected from North Dakota (USA) confirmed that the genotypes screened were true hybrids between A. rubrum and A. saccharinum. These markers were cloned and sequenced. Successful sequences were converted to SCAR markers using specifically designed primers. Overall, the developed diagnostic and specific ISSR and SCAR markers are useful in the certification of these two maple species and their hybrids. They can be used in tracking the introgression of A. rubrum and A. saccharinum DNA in other hybrid trees or populations.

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Zhang, Xinyi, Li Liao, Zhiyong Wang, Changjun Bai, and Jianxiu Liu. "Analysis of Genetic Diversity in Chrysopogon aciculatus Using Intersimple Sequence Repeat and Sequence-related Amplified Polymorphism Markers." HortScience 51, no. 8 (August 2016): 972–79. http://dx.doi.org/10.21273/hortsci.51.8.972.

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Molecular genetic diversity and relationships among 86 Chrysopogon aciculatus (Retz.) Trin. accessions were assessed using intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Twenty-five ISSR markers generated 283 amplification bands, of which 266 were polymorphic. In addition, 576 polymorphic bands were detected from 627 bands amplified using 30 SRAP primers. Both marker types revealed a high level of genetic diversity, with ISSR markers showing a higher proportion of polymorphic loci (PPL; 94%) than SRAP markers (91.87%). The ISSR and SRAP data were significantly correlated (r = 0.8023). Cluster analysis of the separate ISSR and SRAP data sets clustered the accessions into three groups, which generally were consistent with geographic provenance. Cluster analysis of the combined ISSR and SRAP data set revealed four major groups similar to those based solely on ISSR or SRAP markers. The findings demonstrate that ISSR and SRAP markers are reliable and effective tools for analysis of genetic diversity in C. aciculatus.

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Moradkhani, Hoda, Ali Ashraf Mehrabi, Alireza Etminan, and Alireza Pour-Aboughadareh. "Molecular diversity and phylogeny of Triticum-Aegilops species possessing D genome revealed by SSR and ISSR markers." Plant Breeding and Seed Science 71, no. 1 (December 1, 2015): 81–95. http://dx.doi.org/10.1515/plass-2015-0024.

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AbstractThe aim of this study is investigation the applicability of SSR and ISSR markers in evaluating the genetic relationships in twenty accessions ofAegilopsandTriticumspecies with D genome in different ploidy levels. Totally, 119 bands and 46 alleles were detected using ten primers for ISSR and SSR markers, respectively. Polymorphism Information Content values for all primers ranged from 0.345 to 0.375 with an average of 0.367 for SSR, and varied from 0.29 to 0.44 with the average 0.37 for ISSR marker. Analysis of molecular variance (AMOVA) revealed that 81% (ISSR) and 84% (SSR) of variability was partitioned among individuals within populations. Comparing the genetic diversity ofAegilopsandTriticumaccessions, based on genetic parameters, shows that genetic variation ofAe. crassaandAe. tauschiispecies are higher than other species, especially in terms of Nei’s gene diversity. Cluster analysis, based on both markers, separated total accessions in three groups. However, classification based on SSR marker data was not conformed to classification according to ISSR marker data. Principal co-ordinate analysis (PCoA) for SSR and ISSR data showed that, the first two components clarified 53.48% and 49.91% of the total variation, respectively. This analysis (PCoA), also, indicated consistent patterns of genetic relationships for ISSR data sets, however, the grouping of accessions was not completely accorded to their own geographical origins. Consequently, a high level of genetic diversity was revealed from the accessions sampled from different eco-geographical regions of Iran.

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Rakhymberdieva, Zh Sh, A. N. Kaliyeva, and G. D. Medeuova. "MOLECULAR GENETIC PLANT ANALYSIS, ARTEMISIA L. GENUS, WITH ISSR-MARKERS." REPORTS 6, no. 334 (December 15, 2020): 35–41. http://dx.doi.org/10.32014/2020.2518-1483.133.

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. Molecular genetic analysis of three plants of thistle family has been carried out in the scope of this paper (Artemisia karatavica Krasch. & Abolin ex Poljakov, Artemisia cina Berg ex Poljakov, Artemisia porrecta Krasch. ex Poljakov). The plants have been collected in Turkestan region, Shardarinsky district, 15 km north-east of the village Komsomol, and along the road in Turkestan region, Baydibeksky district, 4.5 km south-east of the village Shakpak, and in the steppes in Turkestan region, Aryssky district, 1 km north-east of the village Darmino. In this paper, we used modern methods of molecular biology in order to determine genetic relatedness. ISSR analysis using universal primers has been conducted. ISSR-markers are the most common markers, and they are used for phylogenic analysis. This method is based on amplification of sequences limited by two microsatellite repeats using the primer that is complementary to the sequence of this microsatellite (4-12 repeat units). ISSR (region of the genome between two adjacent, oppositely oriented microsatellites) the sequence of microsatellite medullar part with some (1-3) nucleotides adjacent to the repeat tandem are used as primers. Tens of fragments of locus variety received in PCR are separated by electrophoresis and assessed for the presence or absence (due to marker dominance) of the fragments of a particular size. The main advantage of this type of markers - lack of necessity for knowledge of the sequences during primer designing.

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Archak, S., A. B. Gaikwad, D. Gautam, E. V. V. B. Rao, K. R. M. Swamy, and J. L. Karihaloo. "Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India." Genome 46, no. 3 (June 1, 2003): 362–69. http://dx.doi.org/10.1139/g03-016.

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Nineteen cashew accessions were analysed with 50 random primers, 12 ISSR primers and 6 AFLP primer pairs to compare the efficiency and utility of these techniques for detecting variation in cashew germplasm. Each marker system could discriminate between all of the accessions, albeit with varied efficiency of polymorphism detection. AFLP exhibited maximum discrimination efficiency with a genotype index of 1. The utility of each molecular marker technique, expressed as marker index, was estimated as a function of average band informativeness and effective multiplex ratio. Marker index was calculated to be more than 10 times higher in AFLP than in RAPD and ISSR. Similarity matrices were determined based on the data generated by molecular and morphometric analyses, and compared for congruency. AFLP displayed no correspondence with RAPD and ISSR. Correlation between ISSR and RAPD similarity matrices was low but significant (r = 0.63; p < 0.005). The similarity matrix based on morphometric markers exhibited no correlation with any of the molecular markers. AFLP, with its superior marker utility, was concluded to be the marker of choice for cashew genetic analysis.Key words: Anacardium occidentale, DNA fingerprinting, RAPD, ISSR, AFLP, morphometric.

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Bashandy, Talaat, Ahmed Hussein, Mohamed Solma, Ayman Kassab, and Hatem Hamdon. "Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 68, no. 6 (2020): 929–36. http://dx.doi.org/10.11118/actaun202068060929.

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Molecular markers are the most ideal approach to study genetic diversity. Consequently, we utilized both ISSR and RAPD markers to assess genetic diversity and relationships among three different populations of Farafra, Ossimi and Rahmani Egyptian sheep breeds. Both ISSR and RAPD gave moderate polymorphism 41.3% and 48.51%, respectively. Besides, this value was consistent with the moderate value of the mean of polymorphism information content (0.16 and 0.20, respectively). Farafra-F and Farafra-D populations had the highest similarity which was 0.92 for ISSR and 0.90 for the RAPD marker. Furthermore, ISSR and RAPD constructed dendrogram separated all the studied sheep into two main clusters. All the three populations of Farafra breed combined into one main cluster, while the second cluster contained both Rahmani and Ossimi breeds. The used molecular markers were able to discriminate among evaluated sheep and displayed that Farafra breed more closely related to Ossimi than Rahmani breed.

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Son, Jae-Han, Kyeong-Hoon Kim, Sanghyun Shin, Hag-Sin Kim, Nam-Soo Kim, Jong-Nae Hyun, Sang-In Shim, Choon-Ki Lee, Kwang-Geun Park, and Chon-Sik Kang. "ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification." Plant Breeding and Biotechnology 1, no. 3 (September 30, 2013): 262–69. http://dx.doi.org/10.9787/pbb.2013.1.3.262.

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Katkar, Madhuri, S. S. Mane, and Nivedita Kadam. "Molecular characterization races ofFusarium oxysporumf.sp.ciceriusing RAPD and ISSR markers." Legume Research - An International Journal 38, no. 2 (2015): 246. http://dx.doi.org/10.5958/0976-0571.2015.00083.1.

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Yanpeng, Zhao, Wang Hongmei, Liang Wei, Majid Khayatnezhad, and F. Faisal. "Genetic diversity and relationships among salvia species by ISSR markers." Genetika 53, no. 2 (2021): 559–74. http://dx.doi.org/10.2298/gensr2102559y.

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Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

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Pinar, Hasan, Sezai Ercisli, Mustafa Unlu, Mustafa Bircan, Aydın Uzun, Davut Keles, Filiz Baysal, Halit Atli, and Kadir Yilmaz. "Determination of genetic diversity among some almond accessions." Genetika 47, no. 1 (2015): 13–22. http://dx.doi.org/10.2298/gensr1501013p.

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More recently the use of different molecular markers in fruit species to determine particularly genetic diversity, genetic relationships and cultivar identification has been gained more importance. In the study, 13 randomly amplified polimorfic DNA (RAPD) and 4 inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships among 95 almong accessions (26 foreign cultivars and 69 national cultivars and selections). The all plant material found in Almond Germplasm Repository in Gaziantep, Turkey. Both RAPD and ISSR markers distinguished the almond cultivars and selections in various levels. 17 RAPD and ISSR markers yielded a total of 73 scorable bands, which 51 are polymorphic. The two marker system exhibited variation with regard to average band sizes and polymorphism ratio. The average polymorphism was higher in ISSR (88%) compared to RAPD (74%). RAPD and ISSR marker systems were found to be useful for determining genetic diversity among almong genotypes and cultivars. Combining of two dendrograms obtained through these markers show different clustering of 96 almond specimens without geographical isolation. These results supported that almonds in Turkey indicated considerable genetic diversity.

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Rawat, A., S. Barthwal, and H. S. Ginwal. "Comparative assessment of SSR, ISSR and AFLP markers for characterization of selected genotypes of Himalayan Chir pine (Pinus roxburghii Sarg.) based on resin yield." Silvae Genetica 63, no. 1-6 (December 1, 2014): 94–108. http://dx.doi.org/10.1515/sg-2014-0013.

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AbstractA set of 19 SSR (Simple Sequence Repeats), 9 ISSR (Inter-Simple Sequence Repeats) and 5 AFLP (Amplified Fragment Length Polymorphism) primer combinations were used to evaluate the variability among 53 genotypes of Pinus roxburghii selected based on resin yield from the natural zone of occurrence of this species in Uttarakhand, India. The selected trees of pine varied in resin yield from 0.25 to 8 kg/year/tree. Based on the comparative assessment of SSR, ISSR and AFLP markers, SSR markers were found most polymorphic with an average PIC value of 0.327 and 2.42 alleles per marker, while ISSR markers showed the highest effective multiplex ratio (15.536) and marker index (4.958). AFLP markers showed the maximum resolving power (8.099) which was comparable to the resolving power (8.059) of ISSR markers. UPGMA-based dendrogram using SSR markers revealed more distinct grouping of genotypes on the basis of resin yield as compared to ISSR and AFLP markers. AMOVA by collection site revealed no significant variation among the populations. Whereas, AMOVA by resin yield using SSR, ISSR and AFLP markers revealed FSTvalues to be 0.1096, 0.0483 and 0.2422 indicating moderate, low and great genetic differentiation among the groups. This clearly indicated that the variation at the molecular level was attributed to the resin yield and not the site of collection.

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Rout, G. R., S. K. Senapati, and S. Aparajita. "Study of relationships among twelve Phyllanthus species with the use of molecular markers." Czech Journal of Genetics and Plant Breeding 46, No. 3 (October 14, 2010): 135–41. http://dx.doi.org/10.17221/74/2009-cjgpb.

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The present investigation was undertaken to describe the relationships among twelve species of Phyllanthus collected in India by help of molecular markers. In total, 259 marker loci were assessed, out of which 249 were polymorphic revealing 96.13% polymorphism. Nei's similarity index varied from 0.35 to 0.76 for RAPD (Random Amplified Polymorphic DNA) and from 0.31 to 0.76 for ISSR marker systems. Cluster analysis by the unweighted pair group method (UPGMA) of Dice coefficient of similarity generated dendrogram with more or less similar topology for both the analyses that offered a better explanation for diversity and affinities between the species. The phylogenetic tree obtained from both RAPD and ISSR (Inter Simple Sequence Repeat) markers has divided the 12 species into two groups: group I consisting of only one species Phyllanthus angustifolius (Sw.) Sw and group II with the rest of 11 species. Basically, these results were in compliance with notable morphological characterization. The present study revealed high variation among the species of Phyllanthus and will help to identify different Phyllanthus species.

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Suárez-Contreras, Liliana Yanet, Michell Juliett Arango-Toloza, and Izquel Sánchez-Pabón. "Molecular characterization of mandarin (Citrus reticulata Blanco) using ISSR markers." Revista Colombiana de Ciencias Hortícolas 14, no. 2 (May 1, 2020): 168–77. http://dx.doi.org/10.17584/rcch.2020v14i2.9397.

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In order to establish the molecular diversity of citrus production systems from Villa Sucre (Colombia), mainly "Creole mandarin", the study analyzed C. reticulata farm’s samples using six ISSR molecular markers. A total of 61 polymorphisms were characterized, 42% of them were highly common, with a mid- average polymorphic information content (PIC) of 0.42 indicating a high polymorphic variation. Molecular relations based in Dice coefficient and the UPGMA algorithm, showed the existing genetic relationships between the crop areas, grouping them in five separate clades and two main subgroups (MDS). This is the first molecular classification done in the area, sets the basis for mandarin crop molecular diversity, and provides vital information for mandarin crop management programs.

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Khalifa, Noha, Maher Shehata, Ahmed Abodoma, and Abdullah Alkumbezy. "Molecular analysis of commercial date palm cultivars in Lybia using ISSR and SRAP PCR-based markers." Genetika 48, no. 1 (2016): 307–22. http://dx.doi.org/10.2298/gensr1601307k.

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Little is known about the molecular structure of the date palm (Phoenix dactylifera L.) despite its importance as invaluable drought tolerant crop. Intervarietal variation and cultivar identification are crucial for breeding and gene bank conservation of this plant worldwide. In this work, two PCR based marker systems (ISSR and SRAP) were applied on top quality eight commercial cultivars in Libya (Umfetity, Bekrary, Alhamraya, Sufeer Genab, Alsaeedy Show, Farag Barameel, Majhool Alheelo and Alkhadraya). DNA variations were explored using eleven ISSR and nine combinations of SRAP markers. All markers used generated polymorphic bands among the different cultivars that can be used as molecular markers for their differentiation. The genetic distance between cultivars was also estimated from banding patterns. Our results indicate that ISSR and SRAP systems can efficiently identify and differentiate between the selected cultivars. This work can be used as a model to establish a road map for all date palm cultivars worldwide.

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KIANI, Ghaffar, and Camellia KATALANI. "Divergence in hybrid rice parental lines detected by RAPD and ISSR markers." Acta agriculturae Slovenica 111, no. 2 (October 29, 2018): 369. http://dx.doi.org/10.14720/aas.2018.111.2.12.

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Genetic distance between parental lines used in hybrid rice breeding program was estimated based on information from molecular markers data. Sixteen parents (5 CMS, 5 maintainers and 6 restorers) were analyzed with 15 random amplification polymorphic DNA (RAPD) and 20 inter simple sequence repeat (ISSR) marker. Out of 15 RAPD markers, 9 were polymorphic and 79 bands were generated, of which 28 bands were polymorphic (35 %). By using 10 out of 20 ISSR markers 86 bands were detected, of which 35 bands were polymorphic (41 %). Marker index (RAPD = 1.68; ISSR = 1.88) and percent of polymorphic bands indicated that ISSR markers were relatively more efficient in polymorphism detection. Cluster analysis of the parents based on Jaccard’s genetic similarity and UPGMA method were revealed 3 groups. Restorer lines IR68061 and IR5931 with origin of the Philippines were found in distinct group. Results suggesting the cross between CMS lines Neda and Nemat with restorer lines IR68061 and IR5931, can be used as the best heterotic groups for exploration of heterosis.

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Kafkas, Salih, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker. "Genetic Characterization of Hazelnut (Corylus avellana L.) Cultivars from Turkey Using Molecular Markers." HortScience 44, no. 6 (October 2009): 1557–61. http://dx.doi.org/10.21273/hortsci.44.6.1557.

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Genetic relationships among 18 Turkish hazelnut (Corylus avellana L.) cultivars were investigated using randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) markers. Twenty-five RAPD primers, 25 ISSR primers, and eight AFLP primer pairs generated a total of 434 polymorphic marker loci. The three marker systems were able to differentiate the cultivars. Genetic similarity index values ranged from a high of 0.96 for ‘Kan’ and ‘UzunMusa’ to a low of 0.73 for ‘Yassi Badem’ and ‘Kalinkara’. The genetic relationships were presented as an unweighted pair group method with arithmetic average (UPGMA) dendrogram and a three-dimensional principal coordinate analysis (PCoA) plot. The UPGMA dendrogram showed two main clusters, while PCoA analysis showed three groups. Cultivar-specific markers were produced by all marker systems for 10 cultivars. This study demonstrates the usefulness of molecular markers for identification of hazelnut cultivars.

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Chatterjee, S. N., and T. P. Mohandas. "Identification of ISSR markers associated with productivity traits in silkworm, Bombyx mori L." Genome 46, no. 3 (June 1, 2003): 438–47. http://dx.doi.org/10.1139/g03-024.

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Bombyx mori L., commonly recognised around the world as the mulberry silkworm, is characterized by a wide variability in yield and developmental traits, which have been proven through conventional genetic analysis to be of polygenic nature. A large number of morpho-biochemical traits and RFLP and RAPD markers are mapped on different linkage groups, but to this point very little attention has been given to unravelling the genetics of yield traits. To address this issue, polymorphic profiles of 147 markers generated with 12 ISSR primers on the genomic DNA of 20 silkworm stocks of diverse yield status were subjected to multiple regression and discriminant function analyses (DFA). This led to the identification of eight markers generated by six primers, which demonstrated high β-coefficient indices of 0.451 to 0.940. Furthermore, a significant difference between the yield traits for stocks with and without the specific marker could also be established. The inheritance pattern of one marker, L13800bp, identified at the first step of selection of markers through stepwise regression analyses for five yield parameters is discussed in the context of applying multiple regression analysis for establishing association, if not linkage, between a group of DNA markers and a particular yield trait of polygenic nature and using such markers in molecular marker-assisted breeding programs.Key words: Bombyx mori, silkworm, ISSR markers, yield components.

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Čurn, V., M. Dědouchová, B. Kubátová, J. Malá, P. Máchová, and H. Cvrčková. "Assessment of genetic variability in autochthonous elm populations using ISSR markers." Journal of Forest Science 60, No. 12 (November 27, 2014): 511–18. http://dx.doi.org/10.17221/81/2013-jfs.

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:Genetic diversity between 110 individuals from 6 elm populations (Ulmus minor, U. glabra and U. laevis)was determined using ISSR markers. Altogether 73 ISSR markers were evaluated with the average rate of polymorphic bands of 99.1%, which indicates high genetic diversity between the populations/species. The higher genetic diversity was revealed particularly in the population of U. glabra and this result was supported by the analysis of genetic diversity and differentiation of elm populations. Molecular analyses of ISSR markers allowed to assess the extent of genetic variability of native elm populations and characterize the levels of their genetic diversity and differentiation. Their further use can be seen in conservation and management activities.  

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Shishir Tiwari, Shweta Sao, Antu Kurrey, and Pulak Das. "Isolation and Identification of Molecular Markers for Fingerprinting of Chilli Hybrids & its Parental Lines." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (January 22, 2020): 713–16. http://dx.doi.org/10.26452/ijrps.v11i1.1883.

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Chilli (Capsicum annum) is the predominant sp., which is cultivated in both hot and sweet papers. The maintenance of the genetic purity of chilli plant is a matter of great concern for the breeders. For genetic purity analysis, between true hybrids and off-types, breeders find out morphological differences between them, but this technique is cannot be recognized easily and also costly, tedious to score, and environmentally sensitive. Alternatively, molecular markers based genetic purity analysis can be employed. The molecular marker-based technique was thus used to overcome the conventional method drawbacks. The main objective of the study is to identify informative molecular markers (ISSR and RAPD) capable of distinguishing Chilli hybrids and their parental lines and their utilization in seed purity assessment. Five parental lines of Chilli (i.eCH10, CH12, CH530, CH709, CH734) were used for the production of 3 hybrids. Total 30 ISSR and 8 RAPD primers were selected for the study of 5 parental lines, among them 2ISSR and 1 RAPD primers produced unique fingerprinting across the hybrids. The ISSR marker UBC815 amplified alleles specific to different parental lines(CH10 & CH12) for hybrids (ACH112), The ISSR marker UBC 827, amplified alleles specific to different parental lines(CH709 & CH12) for hybrids (ACH179). Likewise, RAPD primer B20 for hybrid ACH 753 and their parental lines(CH734 & CH530). Thus, the above study showed that the aid of molecular markers is more reliable, highly efficient, and reproducible for assessing fingerprinting of Chilli commercial hybrid seeds with more accuracy.

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Farahani, Farah, Masoud Sheidai, and Fahimeh Koohdar. "Genetic finger printing of cotton cultivars by ISSR molecular markers." Genetika 50, no. 2 (2018): 627–34. http://dx.doi.org/10.2298/gensr1802627f.

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Gossypium hirsutum is one of the main tetraploid cotton species that is cultivated throughout the world. Due to continuous selection of cotton cultivars for specific agronomic traits, the genetic variability within the cultivars decrease that lead to genetic erosion. To tackle the problem of reduced genetic variability, we should track all available genetic diversity within cotton germplasm and use them for inter-specific and intra-specific hybridization and produce new elite cotton cultivars. Therefore, the present study used ISSR molecular markers to illustrate genetic variability in 13 tetraploid cotton genotypes (Gossypium hirsutum L.) and to categorize these genotypes based on genetic affinity. 65 cotton plants were studied. The results identified private bands in the studied genotypes, while Network and STRUCTURE analyses of molecular data obtained grouped the genotypes with genetic affinity together. Some of the genotypes differed in their genetic content from the others; therefore, studying the genetic and agronomic variability within available cultivars is very important and produced data to broaden the gene pool for planning further hybridization in cotton.

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Ghosh, Sanchita, M. Ganga, K. Soorianathasundaram, and Ajit Kumar. "Molecular characterization of jasmine genotypes using RAPD and ISSR markers." Indian Journal of Horticulture 77, no. 1 (2020): 149. http://dx.doi.org/10.5958/0974-0112.2020.00016.x.

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Lombardo, G., R. Schicchi, P. Marino, and F. Palla. "Genetic analysis ofCitrus aurantiumL. (Rutaceae) cultivars by ISSR molecular markers." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 146, sup1 (June 3, 2011): 19–26. http://dx.doi.org/10.1080/11263504.2011.557101.

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Melo, Roberto de A., Luciane V. Resende, Dimas Menezes, Ana Paula A. Beck, José Carlos da Costa, Alisson E. Coutinho, and Ana Verônica S. do Nascimento. "Genetic similarity between coriander genotypes using ISSR markers." Horticultura Brasileira 29, no. 4 (December 2011): 526–30. http://dx.doi.org/10.1590/s0102-05362011000400014.

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With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.

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Vijayan, K., P. P. Srivastava, and A. K. Awasthi. "Analysis of phylogenetic relationship among five mulberry (Morus) species using molecular markers." Genome 47, no. 3 (June 1, 2004): 439–48. http://dx.doi.org/10.1139/g03-147.

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Species identification in mulberry (Morus) continues to be a point of great debate among scientists despite the number of criteria such as floral characters, wood, and leaf anatomical and biochemical characters used to identify the species within this genus. However, no consensus system of classification has emerged. Hence, an investigation was undertaken with inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers to find out the possibility of using these DNA markers to confirm the identity of genotypes in a particular species. Fifteen ISSR and 15 RAPD primers generated 86% and 78% polymorphism, respectively, among 19 mulberry genotypes. The polymorphism among the species varied from 50% to 57% in ISSR markers and 31% to 53% in RAPD markers. Similarity coefficients were higher among the genotypes of M. latifolia, M. bombycis and M. alba. Cluster analyses separated genotypes of M. laevigata and M. indica from those of the other species. Population structure analysis of these species further showed high genetic differentiation coefficients (GST), high heterozygosity between two species (DST), and total heterozygosity among populations (Ht) coupled with considerably low gene flow (Nm) when M. laevigata was paired with other species. Based on these parameters and the result of cluster analysis it is concluded that M. laevigata can be considered as a separate species of mulberry, whereas the other four species may be grouped together and treated as subspecies.Key words: Morus species, genetic marker, ISSR, RAPD, DNA polymorphism, genetic flow.

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Ozyurt, Ibrahim, Yasar Akca, and Sezai Ercisli. "Molecular characterization of Prunus mahaleb L. rootstock canditates by ISSR markers." Genetika 45, no. 3 (2013): 717–26. http://dx.doi.org/10.2298/gensr1303717o.

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Prunus mahaleb is widely used as rootstocks particularly on calcareous and dry soils for both sweet and sour cherry cultivars in Turkey. Genetic diversity and relationships among members of Prunus mahaleb including 29 preselected rootstock candidate accessions from Tokat region in Turkey were investigated by using 15 ISSR markers. The study revealed high genetic diversity among accessions, detecting 138 fragments, of which 103 (75%) were polymorphic. The number of polymorphic bands per primer was between 3-13, with average of 6.86. The primers 890 and 891 gave the highest polymorphism ratio (100%). The UPGMA dendrogram and the principal coordinate analysis revealed a clear differentiation among accessions. Reference rootstock, SL-64 clustered separately. The study demonstrates that ISSRs provide promising marker tools in revealing genetic diversity and relationships in Prunus mahaleb rootstock candidate accessions and can contribute to efficient identification, conservation, and utilization of germplasm for rootstock improvement through conventional as well as molecular breeding approaches.

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M, Tungalag, Ariungerel M, Otgonbayar B, and Myagmarsuren Ya. "Varietal identification study of six wheat varieties using ISSR markers." Mongolian Journal of Agricultural Sciences 23, no. 01 (October 11, 2018): 14–17. http://dx.doi.org/10.5564/mjas.v23i01.1014.

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The identification of cereal and horticultural varieties are important for registration and agricultural systems. The traditional way to variety identification is the recording of morphological characters using descriptors. But molecular markers may serve as a modern and suitable approach to variety identification such as ISSR. The objective of the study was to identify the 17 ISSR primers, 801~849, for varietal identification of six wheat varieties, Darkhan-34, Darkhan-166 (Arvin), Darkhan-131, Darkhan-144, Khalkhgol-1 and Tsogt. As a result of ISSR marker observation on varieties, Arvin can be identified with 817, Khalkhgol-1 with 817 and 827, Tsogt with 822, Darkhan-34 with 830, Darkhan-131 with 830 and 848 and Darkhan-144 with 827, 830 and 848 primers. Among the surveyed ISSR primers, five can be used as variety-specific primers, 817, 827, 822, 830 and 848.

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FELIX, FRANCIVAL CARDOSO, KYVIA PONTES TEIXEIRA DAS CHAGAS, CIBELE DOS SANTOS FERRARI, FÁBIO DE ALMEIDA VIEIRA, and MAURO VASCONCELOS PACHECO. "APPLICATIONS OF ISSR MARKERS IN STUDIES OF GENETIC DIVERSITY OF Pityrocarpa moniliformis." Revista Caatinga 33, no. 4 (October 2020): 1017–24. http://dx.doi.org/10.1590/1983-21252020v33n417rc.

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ABSTRACT Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (Fabaceae) is a native brazilian species with high potential for economic development programs in semiarid regions, mainly related to the production of honey, animal food and firewood. Thus, the objective of this work was to select Inter-Simple Sequence Repeat (ISSR) molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population. For this, 28 ISSR molecular markers were tested, evaluating the total number of loci, polymorphism rate and the Polymorphism Information Content (PIC) for the selected primers, the “Marker Index”, and the “Resolving Power”. Genetic diversity parameters (Nei genetic distance and Shannon index) were evaluated for 30 individuals located in Macaíba, Rio Grande do Norte State, Brazil. Seven primers were selected, which provided 74 loci, with 82% being polymorphic, while the PIC value was 0.344. The Nei genetic distance was 0.244, and the Shannon index was 0.374. Therefore, ISSR molecular markers (UBC 827, 840, 844, 857, 859, 860 and 873) are considered efficient in studying the genetic diversity of populations for the selection of matrices and germplasm banks, and may contribute to the conservation and genetic improvement of P. moniliformis populations.

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Madadi, Meysam, Zabihollah Zamani, and Reza Fatahi. "Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L.) Cultivars, Using ISSR and SSR Markers." Turkish Journal of Agriculture - Food Science and Technology 5, no. 6 (July 12, 2017): 622. http://dx.doi.org/10.24925/turjaf.v5i6.622-628.1100.

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Pomegranate is one of the most important horticultural crops in Iran, and has been cultivated for thousands of years in this country. At this period due to selection of superior cultivars from nature or mutation emerged in these cultivars, and their vegetative propagation, substantial genetic variation has occurred within and among the cultivars. Thus, each cultivar may consist of different clones. According to this issue, diversity within four commercial cultivars of pomegranate was analyzed. Two molecular marker systems including ISSR and SSR were used to evaluate variability between 36 samples of four commercial cultivars. ISSR markers produced 114 amplification products, out of which 97 were polymorphic (83.23%). Mean resolving power was 2.96 for ISSR markers. 19 SSR molecular markers were used, 15 of which amplified polymorphic products, while the remaining ones monomorphic., The number of polymorphic alleles per locus ranged from two to four (average 3.6). The observed and expected heterozygosities ranged from 0.04 to 0.92 and 0.14 to 0.62, respectively. In addition, mean polymorphic information content was 0.45 for SSR loci. Our results showed that commercial Iranian pomegranate have different clones. Therefore, ISSR and SSR markers can be a useful tools for detecting clones of each cultivar.

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Siragusa, Mirko, Fabio De Pasquale, Loredana Abbate, and Nicasio Tusa. "Identification of Sour Orange Accessions and Evaluation of Their Genetic Variability by Molecular Marker Analyses." HortScience 41, no. 1 (February 2006): 84–89. http://dx.doi.org/10.21273/hortsci.41.1.84.

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A collection of 18 accessions of sour orange (Citrus aurantium L.) coming from Sicily and other countries was investigated by two polymerase chain reaction (PCR)-based DNA marker technologies. Ten inter-simple sequence repeat (ISSR) primers and fifteen randomly amplified polymorphic DNA (RAPD) primers were used to identify and to evaluate the genetic variability and relationship of accessions. A total of 111 ISSR and 145 RAPD amplified fragments were used to estimate the Dice's coefficient of similarity for cluster analysis using a unweighted pair-group method using an arithmetic averaging (UPGMA) algorithm. The genetic relationships identified using ISSR and RAPD markers were highly concordant, such that the correlation between ISSR and RAPD genetic distance (GD) estimates was r = 0.93. The ISSR and RAPD analysis of 18 sour orange accessions found a high grade of genetic diversity in foreign accessions, while a low variability was detected in local accessions. Sicilian accessions could be grouped in two distinct clusters, including indistinctly plants from three origin regions. Some markers could be linked to the different growing areas. The ISSR and RAPD molecular reference system seems to be suitable for a fine identification of tightly related plants and the obtained results can form the basis for future setting up of Citrus rootstock genetic improvement projects.

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Nassonova, E., Yu Vasileva, Ya Prishnivskaya, and A. Zhulanov. "Molecular-Genetic Identification and Analysis of Pinus sylvestris L. Populations in Kirov Oblast and Perm Krai." Bulletin of Science and Practice 5, no. 4 (April 15, 2019): 47–57. http://dx.doi.org/10.33619/2414-2948/41/05.

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Molecular–genetic analysis and identification of 5 Pinus sylvestris L. hauls, collected in Kirov oblast and Perm krai was provided. Molecular–genetic analysis was provided with using ISSR–method of DNA polymorphism analysis. In 5 researched hauls of P. sylvestris 126 ISSR–markers were identified, 122 (P95=0.968) were polymorphic. Molecular–genetic identification of researched P. sylvestris populations was provided and molecular–genetic formulas and bar–codes were made. Identification ISSR–markers were identified. Genetic structure and differentiation analysis of P. sylvestris showed that hauls were distributed in three genetic populations.

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Celka, Zbigniew, Monika Szczecińska, and Jakub Sawicki. "Genetic relationships between some of Malva species as determined with ISSR and ISJ markers." Biodiversity: Research and Conservation 19, no. 1 (January 1, 2010): 23–32. http://dx.doi.org/10.2478/v10119-010-0006-2.

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Genetic relationships between some ofMalvaspecies as determined with ISSR and ISJ markersTwo categories of DNA markers were used to determine genetic relationships among eightMalvataxa. A maximum parsimony analysis validated the division of the genusMalvainto the sectionsBismalvaandMalva.The species classified into those sections formed separate clusters.M. moschatawas a distinctive species in the sectionBismalva, as confirmed by previous genetic research based on ITS and cpDNA sequence analyses. The applied markers revealed a very high level of genetic identity betweenM. alcea and M. excisaand enabled molecular identification ofM. alceavar.fastigiata.Speciesspecific markers were determined for the majority of the analyzed species, permitting their molecular identification. A specific marker supporting the differentiation ofM. alceaandM. excisawas not found.

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Мaherovska, О. M. "SELECTION AND ASSESSMENT OF ISSR-MARKERS FOR ANALYSIS OF SEPARATE CATTLE POPULATIONS." Animal Breeding and Genetics 61 (May 27, 2021): 137–45. http://dx.doi.org/10.31073/abg.61.15.

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The article covers molecular genetic analysis of intermicrosatellite DNA sequences of dairy cattle productivity. Molecular markers based on DNA polymorphism were used for this monitoring. Such markers make it possible to assess quickly the genetic polymorphism of taprin in the herd. Іnsofar as a large number of intermicrosatellite repeats is in the genome of cattle, that increases the probability of detecting polymorphic loci. The ISSR markers selected for the study are based on multiclocus synthesis in polymerase chain reaction (PCR) and allow an objective study of the breed and interbreed diversity. And it makes possible to assess quickly and accurately genetic diversity for the presence of genes associated with economically useful traits. The purpose of this work is the selection and evaluation of ISSR-markers for the analysis and study of genetic diversity of Ukrainian and imported breeds of dairy cattle. Samples of biological material from representatives of three herds of cattle (Ukrainian Red-and-White spotted dairy, Montbeliard breed and their crossbreeds) were selected for the study by the method of groups-analogues. For the analysis of this material the generally accepted zootechnical methods of studying of a selection material and methods of an estimation of animals on molecular – genetic markers are included. According to standard methods, DNA was isolated from peripheral blood lymphocytes using a set of reagents "DNA Sorb B". Amplification of total DNA with ISSR primers was performed on a thermal cycler "Tertsyk" ("DNA technology" of the Russian Federation). Electrophoretic separation of DNA fragments was performed in an agarose gel according to conventional methods. The size of the obtained PCR products was detected using a molecular weight marker SM1343. As a result of the study of the biological material of these animals, the obtained ISSR-PCR products were quite heterogeneous. The vast majority of polylocus spectra had clear discrete bands, but there were amplicons without clear discrete bands. Analyzing the results of the study of the genetic structure of animals of the Ukrainian Red-and-White dairy breed, using primers ISSR-1, ISSR-2, ISSR-3 and ISSR-4, the range of obtained PCR products ranges from 250 bp. up to 3000 bp. The range of amplification products in Montbeliard animals has a smaller range and ranges from 250 bp, respectively. up to 1500 bp.The obtained amplicons for the use of primers ISSR-1 and ISSR-2, ISSR-3 and ISSR-4 in the turf of Ukrainian Red-and-White dairy and Montbeliard breeds have sizes from 350 bp to 2000 bp. Having analyzed the information you can determine the distribution of the number and length of DNA fragments using 4 ISSR-markers. The total number of amplified DNA fragments varies depending on the primer from 21 to 106, and their size ranges from 250 BP up to 3000 bp. Based on the analysis of DNA plymorphism, it is possible to assess the heterogeneity of selected populations of cattle. Thus, as a result of studying the genetic structure of animals of two breeds of dairy cattle and their crossbreeds by intermicrosatellite DNA loci, their individual polymorphism was revealed. The amplification products have significant variations depending on the primer used. Primers ISSR-1 and ISSR-2 were the most informative for the analysis of cattle DNA polymorphism.

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Bello-Bello, Jericó J., Lourdes G. Iglesias-Andreu, Susana A. Avilés-Viñas, Eunice Gómez-Uc, Adriana Canto-Flick, and Nancy Santana-Buzzy. "Somaclonal Variation in Habanero Pepper (Capsicum chinense Jacq.) as Assessed ISSR Molecular Markers." HortScience 49, no. 4 (April 2014): 481–85. http://dx.doi.org/10.21273/hortsci.49.4.481.

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Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embryogenic callus system. A cluster analysis was performed to express in the form of dendrogram the relationships among different regeneration systems and the genetic variability detected. Genetic distance analysis indicated that our regeneration protocols are inappropriate for micropropagation, conservation, or genetic transformation; however, they may be applicable to breeding. This is the first report on the use of molecular analysis to evaluate genetic variation of in vitro-regenerated plants of Habanero pepper using ISSR markers.

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Pharmawati, M., G. Yan, and I. J. McFarlane. "Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae)." Australian Systematic Botany 17, no. 1 (2004): 49. http://dx.doi.org/10.1071/sb03016.

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The potential of RAPD and ISSR markers to construct molecular relationships of Grevillea was evaluated with 23 RAPD and 12 ISSR primers. The 16 genotypes representing 12 species and 3 subspecies of Grevillea were sampled from the collection of the Mt Anann Botanic Garden, NSW. RAPD and ISSR assays generated a total of 401 RAPD and 280 ISSR fragments. High frequencies of polymorphisms, 99.39% for RAPD and 99.51% for ISSR, were detected by both markers. Three statistical approaches were employed to construct phylogenetic relationships from combined RAPD and ISSR data. Cluster analysis by the unweighted pair group method (UPGMA) of Jaccard's similarity and Neighbour-Joining analysis of total character difference generated dendograms with similar topology. Parsimony analysis also generated a tree that was in broad agreement with the two dendograms. The phylogenetic trees divided the Grevillea species studied into three groups. Group A consisted of G. buxifolia subsp. buxifolia, G. phylicoides and G. sphacelata. In group B, G. mucronulata was grouped together with G. montana, while G. diffusa, G. humilis, G. linearifolia, G. molyneuxii, G. oldei, G. sericea and G. speciosa formed group C. This molecular result was comparable to groupings suggested by a previous author (Makinson 2000) based on morphological characteristics. However, in contrast to the morphological taxonomy, molecular phylogeny suggests that G. oldei and G. speciosa belong to the same subgroup sensu Makinson (2000), whereas G. linearifolia and G. molyneuxii should not be placed in their originally suggested subgroups sensu Makinson (2000). The present study is the first published report on molecular relationships of Grevillea and can be considered as an initial point for further research on the genetic relationships and evolution of Grevillea.

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Chokheli, Vasiliy, Boris Kozlovsky, Mikhail Sereda, Vladimir Lysenko, Igor Fesenko, Tatiana Varduny, Olga Kapralova, and Elena Bondarenko. "Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers." Journal of Botany 2016 (February 22, 2016): 1–7. http://dx.doi.org/10.1155/2016/7910451.

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Quercus robur L. is a valuable wood species having long ontogeny and promising to create long-living artificial plantings of recreational and ameliorative purposes in the steppes zone of Russia and other countries. In this work we have performed the genotyping of varieties of Quercus robur L. obtained from collection of Botanical Garden of Southern Federal University using intersimple sequence repeat (ISSR) molecular markers. The most polymorphic ISSR-marker (GA) 8YC was found in the collection. The polymorphic DNA markers identified in the present study can be used for the future breeding works to obtain valuable genotypes of Quercus genus. In addition we have performed DNA fingerprinting of the prospective sample of the variety Q. robur var. tardiflora Czern.

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JHANG, T., M. KAUR, P. KALIA, and T. R. SHARMA. "Efficiency of different marker systems for molecular characterization of subtropical carrot germplasm." Journal of Agricultural Science 148, no. 2 (January 22, 2010): 171–81. http://dx.doi.org/10.1017/s0021859609990591.

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SUMMARYGenetic variability in carrots is a consequence of allogamy, which leads to a high level of inbreeding depression, affecting the development of new varieties. To understand the extent of genetic variability in 40 elite indigenous breeding lines of subtropical carrots, 48 DNA markers consisting of 16 inter simple sequence repeats (ISSRs), 10 universal rice primers (URPs), 16 random amplification of polymorphic DNA (RAPD) and six simple sequence repeat (SSR) markers were used. These 48 markers amplified a total of 591 bands, of which 569 were polymorphic (0·96). Amplicon size ranged from 200 to 3500 base pairs (bp) in ISSR, RAPD and URPs markers and from 100 to 300 bp in SSR markers. The ISSR marker system was found to be most efficient with (GT)n motifs as the most abundant SSR loci in the carrot genome. The unweighted pair group method with arithmetic mean (UPGMA) analysis of the combined data set of all the DNA markers obtained by four marker systems classified 40 genotypes in two groups with 0·45 genetic similarity with high Mantel matrix correlation (r=0·92). The principal component analysis (PCA) of marker data also explained 0·55 of the variation by first three components. Molecular diversity was very high and non-structured in these open-pollinated genotypes. The study demonstrated for the first time that URPs can be used successfully in genetic diversity analysis of tropical carrots. In addition, an entirely a new set of microsatellite markers, derived from the expressed sequence tags (ESTs) sequences of carrots, has been developed and utilized successfully.

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YOUSEFIAZARKHANIAN, Masoumeh, Ali ASGHARI, Jafar AHMADI, Behvar ASGHARI, and Ali Ashraf JAFARI. "Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44, no. 2 (December 14, 2016): 431–36. http://dx.doi.org/10.15835/nbha44210391.

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The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Neiâ€™s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

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Guasmi, Ferdaous, Walid Elfalleh, Hédia Hannachi, Khadija Fères, Leila Touil, Nidhal Marzougui, Tebra Triki, and Ali Ferchichi. "The Use of ISSR and RAPD Markers for Genetic Diversity among South Tunisian Barley." ISRN Agronomy 2012 (January 3, 2012): 1–10. http://dx.doi.org/10.5402/2012/952196.

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Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) were assayed to determine the genetic diversity of 80 barley specimens from South Tunisia. The ISSR primers showed variation in the percentage of polymorphism, band informativeness (Ib), and resolving power (Rp). The percentage of polymorphism is 66.67%, the average Ib ranged from 0.24 to 0.39, while Rp ranged from 0.74 to 1.16. In RAPD analysis, three primers yielded a total of 17 scorable bands, which are all polymorphic. The three polymorphic primers exhibited variation with regard to average band informativeness (AvIb) and resolving power (Rp). RAPD and ISSR marker systems were found to be useful for the genetic diversity among the barley specimens. The two dendrograms obtained through these markers show different clustering of 80 barely specimens, but we noted that some clusters were similar in some cases. A poor correlation () was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships. Therefore, the ISSR and RAPD molecular markers show two genetic grouping of studied barely specimens.

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El Harfi, Meriem, Jamal Charafi, Karim Houmanat, Hafida Hanine, and Abdelghani Nabloussi. "Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers." OCL 28 (2021): 3. http://dx.doi.org/10.1051/ocl/2020072.

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There is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated using ISSR (Inter Sequence Simple Repeats) markers as a simple method to reveal polymorphism among them. A total of 130 individuals representing 31 populations were sampled. Twenty-four ISSR primers were used for analysis of individuals representing the 31 different sesame populations grown in different agroclimatic zones of Tadla, accounting for 90% of sesame cultivation area in Morocco. Indeed, seven primers showed legible and reproducible genomic profiles with an interesting number of bands. A total of 57 bands were obtained with ISSR primers, of which 47 were polymorphic. PIC (Polymorphic Information Content) ranged from 0.002 to 0.350, showing that ISSR markers are informative and relevant for discriminating the populations evaluated. The similarity coefficient of ISSR data ranged from 0.509 to 1, with an average of 0.870. The results obtained showed that Moroccan sesame populations are characterized by a low genetic diversity, suggesting a genetic proximity among them. Therefore, new germplasm should be either introduced from diverse geographical origins or created through mutagenesis breeding in order to broaden the existing genetic variability.

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Luz, G. A., S. O. Gomes, R. B. Araújo Neto, M. S. C. B. Nascimento, and P. S. C. Lima. "Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers." Genetics and Molecular Research 14, no. 4 (2015): 15242–48. http://dx.doi.org/10.4238/2015.november.25.12.

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Asadova, Almas, Sevda Babayeva, Vusala Izzatullayeva, Seadet Akbarova, Gunel Aghazade, Ilhama Mirzaliyeva, and Mehraj Abbasov. "Molecular characterization of L. sativus L. collection based on ISSR markers." Genetika 52, no. 2 (2020): 777–86. http://dx.doi.org/10.2298/gensr2002777a.

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Kumar, Mukesh, Navneet Kumar, Sunil Malik, Arvind Kumar, and Vipin Kumar. "Molecular Characterization of Chickpea (Cicer ArietinumL) Through Rapd and Issr Markers." Progressive Agriculture 15, no. 2 (2015): 277. http://dx.doi.org/10.5958/0976-4615.2015.00018.6.

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Nam, Jae-Ik, Sea-Hyun Kim, and Chul-Woo Kim. "Genetic Variation Analysis of Chinese Jujube Cultivars Using ISSR Molecular Markers." Plant Breeding and Biotechnology 7, no. 3 (September 1, 2019): 200–207. http://dx.doi.org/10.9787/pbb.2019.7.3.200.

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Raja, M. Bharathi, K. Rajamani, J. Suresh, A. John Joel, and D. Uma. "Molecular characterization of vetiver [Chrysopogon zizanioides Roberty] genotypes using ISSR markers." Medicinal Plants - International Journal of Phytomedicines and Related Industries 11, no. 3 (2019): 246. http://dx.doi.org/10.5958/0975-6892.2019.00033.9.

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PHARMAWATI, MADE, GUIJUN YAN, and PATRICK M. FINNEGAN. "Molecular Variation and Fingerprinting of Leucadendron Cultivars (Proteaceae) by ISSR Markers." Annals of Botany 95, no. 7 (March 24, 2005): 1163–70. http://dx.doi.org/10.1093/aob/mci127.

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Hiremath, Channayya, Roja Philip, and Velusamy Sundaresan. "Molecular characterization of India Ginseng Withania somnifera (L) using ISSR markers." Molecular Biology Reports 48, no. 5 (May 2021): 3971–77. http://dx.doi.org/10.1007/s11033-021-06397-8.

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Journal articles on the topic 'Molecular markers ISSR'

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