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Relevant bibliographies by topics / Molecular pairs based on P-alkyl fragment

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Academic literature on the topic 'Molecular pairs based on P-alkyl fragment'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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Journal articles on the topic "Molecular pairs based on P-alkyl fragment"

1

Cieślińska, Mirosława, and Aneliya Borisova. "MOLECULAR CHARACTERIZATION OF ‘Candidatus PHYTOPLASMA MALI’ STRAINS FROM BULGARIA AND POLAND." Acta Scientiarum Polonorum Hortorum Cultus 18, no. 5 (2019): 181–88. http://dx.doi.org/10.24326/asphc.2019.5.18.

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During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene amplified in nested PCR using primer pair P1/P7 followed by R16F2n/R16R2 and F1/B6 primer pairs. The nitroreductase and rhodonase like genes and ribosomal protein genes rpl22 and rps3 were then analyzed using PCR-RFLP technique to study the genetic variability of the

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2

Zhang, A. W., G. L. Hartman, B. Curio-Penny, W. L. Pedersen, and K. B. Becker. "Molecular Detection of Diaporthe phaseolorum and Phomopsis longicolla from Soybean Seeds." Phytopathology® 89, no. 9 (1999): 796–804. http://dx.doi.org/10.1094/phyto.1999.89.9.796.

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Species-specific detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds was accomplished using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan chemistry. To use these detection systems, fungal DNA was released from soybean seed coats using an ultrasonic processor to break the cells. DNA fragment lengths ranged from 200 to 1,200 base pairs (bp), with the majority of fragments <500 bp. Based on DNA sequences of the internal transcribed spacer (ITS) regions of ribosomal DNA, three TaqMan primer/probe sets were designed. Primer/pr

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3

Krauss, Siegfried L., and Rod Peakall. "An Evaluation of the AFLP Fingerprinting Technique for the Analysis of Paternity in Natural Populations of Persoonia mollis (Proteaceae)." Australian Journal of Botany 46, no. 4 (1998): 533. http://dx.doi.org/10.1071/bt97043.

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The accurate assignment of paternity in natural plant populations is required to address important issues in evolutionary biology, such as the factors that affect reproductive success. Newly developed molecular fingerprinting techniques offer the potential to address these aims. Here, we evaluate the utility of a new PCR-based multi-locus fingerprinting technique called Amplified Fragment Length Polymorphism (AFLP) for paternity studies in Persoonia mollis (Proteaceae). AFLPs were initially scored for five individuals from three taxonomic levels for 64 primer pairs: between species (P. mollis

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4

Zhang, Xue, Yuanyu Shan, Jingling Li, Qiulin Qin, Jie Yu, and Hongping Deng. "Assembly of the Complete Mitochondrial Genome of Pereskia aculeata Revealed That Two Pairs of Repetitive Elements Mediated the Recombination of the Genome." International Journal of Molecular Sciences 24, no. 9 (2023): 8366. http://dx.doi.org/10.3390/ijms24098366.

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Pereskia aculeata is a potential new crop species that has both food and medicinal (antinociceptive activity) properties. However, comprehensive genomic research on P. aculeata is still lacking, particularly concerning its organelle genome. In this study, P. aculeata was studied to sequence the mitochondrial genome (mitogenome) and to ascertain the assembly, informational content, and developmental expression of the mitogenome. The findings revealed that the mitogenome of P. aculeata is circular and measures 515,187 bp in length with a GC content of 44.05%. It contains 52 unique genes, includi

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5

Choudhury, Yukti, Pannapa Pinweha, Jonathan Poh, Jing Shan Lim, and Min-Han Tan. "Early detection of cancer using cell-free DNA (cfDNA) size analysis on a multiplexed amplicon-based next-generation sequencing (NGS) platform." Journal of Clinical Oncology 40, no. 16_suppl (2022): 3041. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.3041.

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3041 Background: Tumor-derived cfDNA fragments are observed to be shorter in length than normal cfDNA. This size (length) difference can be analyzed as a tumor-specific signal. Whole genome or probe hybridization-based NGS methods can capture cfDNA fragments of native sizes. However, amplicon-based NGS assays are not directly amenable to cfDNA size analysis due to predetermination of amplicon sizes by design. Here we present a method to extract relative distribution of cfDNA fragment lengths from a targeted amplicon-based assay and show its utility in cancer detection. Methods: The LiquidHALLM

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6

Choudhury, Yukti, Pannapa Pinweha, Jonathan Poh, Jing Shan Lim, and Min-Han Tan. "Early detection of cancer using cell-free DNA (cfDNA) size analysis on a multiplexed amplicon-based next-generation sequencing (NGS) platform." Journal of Clinical Oncology 40, no. 16_suppl (2022): 3041. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.3041.

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3041 Background: Tumor-derived cfDNA fragments are observed to be shorter in length than normal cfDNA. This size (length) difference can be analyzed as a tumor-specific signal. Whole genome or probe hybridization-based NGS methods can capture cfDNA fragments of native sizes. However, amplicon-based NGS assays are not directly amenable to cfDNA size analysis due to predetermination of amplicon sizes by design. Here we present a method to extract relative distribution of cfDNA fragment lengths from a targeted amplicon-based assay and show its utility in cancer detection. Methods: The LiquidHALLM

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7

Massawe, F. J., M. Dickinson, J. A. Roberts, and S. N. Azam-Ali. "Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces revealed by AFLP markers." Genome 45, no. 6 (2002): 1175–80. http://dx.doi.org/10.1139/g02-093.

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Bambara groundnut (Vigna subterranea (L.) Verdc), an African indigenous legume, is popular in most parts of Africa. The present study was undertaken to establish genetic relationships among 16 cultivated bambara groundnut landraces using fluorescence-based amplified fragment length polymorphism (AFLP) markers. Seven selective primer combinations generated 504 amplification products, ranging from 50 to 400 bp. Several landrace-specific products were identified that could be effectively used to produce landrace-specific markers for identification purposes. On average, each primer combination gen

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8

Nkongolo, K. K., P. Michael, and W. S. Gratton. "Identification and characterization of RAPD markers inferring genetic relationships among Pine species." Genome 45, no. 1 (2002): 51–58. http://dx.doi.org/10.1139/g01-121.

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Total genomic DNAs were extracted from several populations of pine species and amplified using oligonucleotides of random sequences. Polymorphism in random amplified polymorphic DNA (RAPD) markers was high and sufficient in distinguishing each of the species. Genetic relationships among eight pine species (Pinus sylvestris, Pinus strobus, Pinus rigida, Pinus resinosa, Pinus nigra, Pinus contorta, Pinus monticola, and Pinus banksiana) from different provenances were analyzed. The degree of band sharing was used to evaluate genetic distance between species and to construct a phylogenetic tree. I

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9

Ahmed, Md Sagir, Sujan Kumar Datta, and Ayesha Akhter Zhilik. "Molecular diversity of freshwater fishes of Bangladesh assessed by DNA barcoding." Bangladesh Journal of Zoology 48, no. 1 (2020): 1–19. http://dx.doi.org/10.3329/bjz.v48i1.47872.

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This study represents the comprehensive molecular identification of freshwater fishes of Bangladesh based on a fragment of the cytochrome c oxidase subunit I (COI) gene in the mitochondrial genome. A total of 315 mitochondrial COI barcode sequences were obtained from 153 species,114 genera, 49 families and 16 orders of fishes. The mean length of the sequences was 652 base pairs. For all the samples, %G was significantly lower compared to the other nucleotides and %GC was lower compared to %AT (p-value ˂ 0.05). Also, a significantly lower %GC content was observed in second and third codon posit

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10

Kolmer, J. A. "Molecular polymorphism and virulence phenotypes of the wheat leaf rust fungus Puccinia triticina in Canada." Canadian Journal of Botany 79, no. 8 (2001): 917–26. http://dx.doi.org/10.1139/b01-075.

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Sixty-nine Puccinia triticina isolates from the eastern region of Ontario and Quebec, the prairie region of Manitoba and Saskatchewan, and Alberta were tested for virulence to 22 near-isogenic Thatcher wheat lines in seedling tests and the Thatcher line with Lr22b in adult plant tests. The isolates were also tested for amplified fragment length polymorphism (AFLP) using 10 specific amplification primer pairs. Thirty-seven distinct virulence phenotypes were identified using the Thatcher lines and 69 molecular phenotypes were identified with 164 AFLP markers. The correlation of isolate grouping

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