To see the other types of publications on this topic, follow the link: Molecular pairs based on P-alkyl fragment.

Journal articles on the topic 'Molecular pairs based on P-alkyl fragment'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Molecular pairs based on P-alkyl fragment.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Cieślińska, Mirosława, and Aneliya Borisova. "MOLECULAR CHARACTERIZATION OF ‘Candidatus PHYTOPLASMA MALI’ STRAINS FROM BULGARIA AND POLAND." Acta Scientiarum Polonorum Hortorum Cultus 18, no. 5 (2019): 181–88. http://dx.doi.org/10.24326/asphc.2019.5.18.

Full text
Abstract:
During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene amplified in nested PCR using primer pair P1/P7 followed by R16F2n/R16R2 and F1/B6 primer pairs. The nitroreductase and rhodonase like genes and ribosomal protein genes rpl22 and rps3 were then analyzed using PCR-RFLP technique to study the genetic variability of the
APA, Harvard, Vancouver, ISO, and other styles
2

Zhang, A. W., G. L. Hartman, B. Curio-Penny, W. L. Pedersen, and K. B. Becker. "Molecular Detection of Diaporthe phaseolorum and Phomopsis longicolla from Soybean Seeds." Phytopathology® 89, no. 9 (1999): 796–804. http://dx.doi.org/10.1094/phyto.1999.89.9.796.

Full text
Abstract:
Species-specific detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds was accomplished using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan chemistry. To use these detection systems, fungal DNA was released from soybean seed coats using an ultrasonic processor to break the cells. DNA fragment lengths ranged from 200 to 1,200 base pairs (bp), with the majority of fragments <500 bp. Based on DNA sequences of the internal transcribed spacer (ITS) regions of ribosomal DNA, three TaqMan primer/probe sets were designed. Primer/pr
APA, Harvard, Vancouver, ISO, and other styles
3

Krauss, Siegfried L., and Rod Peakall. "An Evaluation of the AFLP Fingerprinting Technique for the Analysis of Paternity in Natural Populations of Persoonia mollis (Proteaceae)." Australian Journal of Botany 46, no. 4 (1998): 533. http://dx.doi.org/10.1071/bt97043.

Full text
Abstract:
The accurate assignment of paternity in natural plant populations is required to address important issues in evolutionary biology, such as the factors that affect reproductive success. Newly developed molecular fingerprinting techniques offer the potential to address these aims. Here, we evaluate the utility of a new PCR-based multi-locus fingerprinting technique called Amplified Fragment Length Polymorphism (AFLP) for paternity studies in Persoonia mollis (Proteaceae). AFLPs were initially scored for five individuals from three taxonomic levels for 64 primer pairs: between species (P. mollis
APA, Harvard, Vancouver, ISO, and other styles
4

Zhang, Xue, Yuanyu Shan, Jingling Li, Qiulin Qin, Jie Yu, and Hongping Deng. "Assembly of the Complete Mitochondrial Genome of Pereskia aculeata Revealed That Two Pairs of Repetitive Elements Mediated the Recombination of the Genome." International Journal of Molecular Sciences 24, no. 9 (2023): 8366. http://dx.doi.org/10.3390/ijms24098366.

Full text
Abstract:
Pereskia aculeata is a potential new crop species that has both food and medicinal (antinociceptive activity) properties. However, comprehensive genomic research on P. aculeata is still lacking, particularly concerning its organelle genome. In this study, P. aculeata was studied to sequence the mitochondrial genome (mitogenome) and to ascertain the assembly, informational content, and developmental expression of the mitogenome. The findings revealed that the mitogenome of P. aculeata is circular and measures 515,187 bp in length with a GC content of 44.05%. It contains 52 unique genes, includi
APA, Harvard, Vancouver, ISO, and other styles
5

Choudhury, Yukti, Pannapa Pinweha, Jonathan Poh, Jing Shan Lim, and Min-Han Tan. "Early detection of cancer using cell-free DNA (cfDNA) size analysis on a multiplexed amplicon-based next-generation sequencing (NGS) platform." Journal of Clinical Oncology 40, no. 16_suppl (2022): 3041. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.3041.

Full text
Abstract:
3041 Background: Tumor-derived cfDNA fragments are observed to be shorter in length than normal cfDNA. This size (length) difference can be analyzed as a tumor-specific signal. Whole genome or probe hybridization-based NGS methods can capture cfDNA fragments of native sizes. However, amplicon-based NGS assays are not directly amenable to cfDNA size analysis due to predetermination of amplicon sizes by design. Here we present a method to extract relative distribution of cfDNA fragment lengths from a targeted amplicon-based assay and show its utility in cancer detection. Methods: The LiquidHALLM
APA, Harvard, Vancouver, ISO, and other styles
6

Choudhury, Yukti, Pannapa Pinweha, Jonathan Poh, Jing Shan Lim, and Min-Han Tan. "Early detection of cancer using cell-free DNA (cfDNA) size analysis on a multiplexed amplicon-based next-generation sequencing (NGS) platform." Journal of Clinical Oncology 40, no. 16_suppl (2022): 3041. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.3041.

Full text
Abstract:
3041 Background: Tumor-derived cfDNA fragments are observed to be shorter in length than normal cfDNA. This size (length) difference can be analyzed as a tumor-specific signal. Whole genome or probe hybridization-based NGS methods can capture cfDNA fragments of native sizes. However, amplicon-based NGS assays are not directly amenable to cfDNA size analysis due to predetermination of amplicon sizes by design. Here we present a method to extract relative distribution of cfDNA fragment lengths from a targeted amplicon-based assay and show its utility in cancer detection. Methods: The LiquidHALLM
APA, Harvard, Vancouver, ISO, and other styles
7

Massawe, F. J., M. Dickinson, J. A. Roberts, and S. N. Azam-Ali. "Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces revealed by AFLP markers." Genome 45, no. 6 (2002): 1175–80. http://dx.doi.org/10.1139/g02-093.

Full text
Abstract:
Bambara groundnut (Vigna subterranea (L.) Verdc), an African indigenous legume, is popular in most parts of Africa. The present study was undertaken to establish genetic relationships among 16 cultivated bambara groundnut landraces using fluorescence-based amplified fragment length polymorphism (AFLP) markers. Seven selective primer combinations generated 504 amplification products, ranging from 50 to 400 bp. Several landrace-specific products were identified that could be effectively used to produce landrace-specific markers for identification purposes. On average, each primer combination gen
APA, Harvard, Vancouver, ISO, and other styles
8

Nkongolo, K. K., P. Michael, and W. S. Gratton. "Identification and characterization of RAPD markers inferring genetic relationships among Pine species." Genome 45, no. 1 (2002): 51–58. http://dx.doi.org/10.1139/g01-121.

Full text
Abstract:
Total genomic DNAs were extracted from several populations of pine species and amplified using oligonucleotides of random sequences. Polymorphism in random amplified polymorphic DNA (RAPD) markers was high and sufficient in distinguishing each of the species. Genetic relationships among eight pine species (Pinus sylvestris, Pinus strobus, Pinus rigida, Pinus resinosa, Pinus nigra, Pinus contorta, Pinus monticola, and Pinus banksiana) from different provenances were analyzed. The degree of band sharing was used to evaluate genetic distance between species and to construct a phylogenetic tree. I
APA, Harvard, Vancouver, ISO, and other styles
9

Ahmed, Md Sagir, Sujan Kumar Datta, and Ayesha Akhter Zhilik. "Molecular diversity of freshwater fishes of Bangladesh assessed by DNA barcoding." Bangladesh Journal of Zoology 48, no. 1 (2020): 1–19. http://dx.doi.org/10.3329/bjz.v48i1.47872.

Full text
Abstract:
This study represents the comprehensive molecular identification of freshwater fishes of Bangladesh based on a fragment of the cytochrome c oxidase subunit I (COI) gene in the mitochondrial genome. A total of 315 mitochondrial COI barcode sequences were obtained from 153 species,114 genera, 49 families and 16 orders of fishes. The mean length of the sequences was 652 base pairs. For all the samples, %G was significantly lower compared to the other nucleotides and %GC was lower compared to %AT (p-value ˂ 0.05). Also, a significantly lower %GC content was observed in second and third codon posit
APA, Harvard, Vancouver, ISO, and other styles
10

Kolmer, J. A. "Molecular polymorphism and virulence phenotypes of the wheat leaf rust fungus Puccinia triticina in Canada." Canadian Journal of Botany 79, no. 8 (2001): 917–26. http://dx.doi.org/10.1139/b01-075.

Full text
Abstract:
Sixty-nine Puccinia triticina isolates from the eastern region of Ontario and Quebec, the prairie region of Manitoba and Saskatchewan, and Alberta were tested for virulence to 22 near-isogenic Thatcher wheat lines in seedling tests and the Thatcher line with Lr22b in adult plant tests. The isolates were also tested for amplified fragment length polymorphism (AFLP) using 10 specific amplification primer pairs. Thirty-seven distinct virulence phenotypes were identified using the Thatcher lines and 69 molecular phenotypes were identified with 164 AFLP markers. The correlation of isolate grouping
APA, Harvard, Vancouver, ISO, and other styles
11

Sakulsathaporn, Arpakorn, Intira Choradet, and Chanram Roopkham. "Genetic Diversity of the Endemic Species Phanera sirindhorniae in the Mekong Basin of Thailand Based on ISSR Markers." Trends in Sciences 20, no. 12 (2023): 7053. http://dx.doi.org/10.48048/tis.2023.7053.

Full text
Abstract:
Phanera sirindhorniae, an endemic species to the Mekong Basin’s upper northeastern region in Thailand, is notable for its therapeutic properties in alleviating pain and discomfort. The objective of this study was to assess the genetic diversity of P. sirindhorniae derived from four natural sources, specifically Sakon Nakhon, Nakhon Phanom, Bueng Kan and Mukdahan provinces, utilizing Inter-Simple Sequence Repeat (ISSR) markers. A total of 51 samples were analyzed, and 10 primers exhibited distinct, robust amplified fragments and polymorphism. The amplified fragment sizes ranged from 170 to 1,30
APA, Harvard, Vancouver, ISO, and other styles
12

Seemüller, Erich, and Bernd Schneider. "Differences in Virulence and Genomic Features of Strains of ‘Candidatus Phytoplasma mali’, the Apple Proliferation Agent." Phytopathology® 97, no. 8 (2007): 964–70. http://dx.doi.org/10.1094/phyto-97-8-0964.

Full text
Abstract:
Root and shoot samples from 24 symptomatic or nonsymptomatic apple trees infected with ‘Candidatus Phytoplasma mali’ were collected at different locations in Germany and France and used to inoculate rootstock M11 top grafted with cv. Golden Delicious. Inoculated trees were monitored over a 12-year period for apple proliferation (AP) symptoms and categorized as not or slightly, moderately, or severely affected. Based on symptomatology, the phytoplasma strains were defined as being avirulent to mildly, moderately, or highly virulent. Determination of phytoplasma titers by quantitative polymerase
APA, Harvard, Vancouver, ISO, and other styles
13

Martin, Frank N., Paul W. Tooley, and Cheryl Blomquist. "Molecular Detection of Phytophthora ramorum, the Causal Agent of Sudden Oak Death in California, and Two Additional Species Commonly Recovered from Diseased Plant Material." Phytopathology® 94, no. 6 (2004): 621–31. http://dx.doi.org/10.1094/phyto.2004.94.6.621.

Full text
Abstract:
Sudden oak death is a disease currently devastating forest ecosystems in several coastal areas of California. The pathogen causing this is Phy-tophthora ramorum, although species such as P. nemorosa and P. pseudo-syringae often are recovered from symptomatic plants as well. A molecular marker system was developed based on mitochondrial sequences of the cox I and II genes for detection of Phytophthora spp. in general, and P. ramorum, P. nemorosa, and P. pseudosyringae in particular. The first-round multiplex amplification contained two primer pairs, one for amplification of plant sequences to s
APA, Harvard, Vancouver, ISO, and other styles
14

Friden, P., and P. Schimmel. "LEU3 of Saccharomyces cerevisiae activates multiple genes for branched-chain amino acid biosynthesis by binding to a common decanucleotide core sequence." Molecular and Cellular Biology 8, no. 7 (1988): 2690–97. http://dx.doi.org/10.1128/mcb.8.7.2690-2697.1988.

Full text
Abstract:
LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that regulates transcription of a group of genes involved in leucine biosynthesis and has been shown to bind specifically to a 114-base-pair DNA fragment of the LEU2 upstream region (P. Friden and P. Schimmel, Mol. Cell. Biol. 7:2707-2717, 1987). We show here that, in addition to LEU2, LEU3 binds in vitro to sequences in the promoter regions of LEU1, LEU4, ILV2, and, by inference, ILV5. The largely conserved decanucleotide core sequence shared by the binding sites in these genes is CCGGNNCCGG. Methylation interference footp
APA, Harvard, Vancouver, ISO, and other styles
15

Maharani, Andi Nacisa Malfin Savina, Ridwan Sala, Abdul Hamid A. Toha, et al. "Morphological and Genetic Characteristics of Red Snapper (Lutjanidae) in Nabire Waters." ILMU KELAUTAN: Indonesian Journal of Marine Sciences 30, no. 1 (2025): 83–91. https://doi.org/10.14710/ik.ijms.30.1.83-91.

Full text
Abstract:
The Lutjanidae family has a wide range of varieties, posing challenges in their morphological identification. Molecular identification is crucial for augmenting the current morphological data as a comprehensive database for documenting the presence of economically significant fish species in Nabire water. This article provides a comprehensive analysis of the morphological and genetic characteristics of red snapper species, as well as assessment of the evolutionary connections among snapper fish found in the water. This study aims to analyze the morphological and genetic characteristics of red
APA, Harvard, Vancouver, ISO, and other styles
16

Patel, Jaimin S., Steven W. Meinhardt, Helge Sierotzki, Gerd Stammler, Neil C. Gudmestad, and Tika B. Adhikari. "A Two-Step Molecular Detection Method for Pyrenophora tritici-repentis Isolates Insensitive to QoI Fungicides." Plant Disease 95, no. 12 (2011): 1558–64. http://dx.doi.org/10.1094/pdis-05-11-0413.

Full text
Abstract:
Tan spot, caused by Pyrenophora tritici-repentis, is an important disease of wheat worldwide. To manage tan spot, quinone outside inhibitor (QoI) fungicides such as azoxystrobin and pyraclostrobin have been applied in many countries. QoI fungicides target the cytochrome b (cyt b) site in complex III of mitochondria and, thus, pose a serious risk for resistance development. The resistance mechanism to QoI fungicides is mainly due to point mutations in the cyt b gene. The objective of this study was to develop a molecular detection method for the four currently known mutations responsible for sh
APA, Harvard, Vancouver, ISO, and other styles
17

Deng, Quanqing. "Development of a SCAR Marker Based on SCoT Polymorphisms for Sugarcane Smut Resistance." International Journal of Agriculture and Biology 25, no. 06 (2021): 1272–80. http://dx.doi.org/10.17957/ijab/15.1789.

Full text
Abstract:
Sugarcane smut caused by Sporisorium scitanmineumis the most severe sugarcane disease that causes major economic losses in sugarcane production in China, and disease resistance breeding is an important way of preventing and controlling this disease. In this study, BC3F1lines derived from the cross between YC 73-226 and YCE 06-111 were used to generate sugarcane smut-resistant and -susceptible gene pools using bulked segregant analysis (BSA). Eighty-nine random primers of start codon targeted (SCoT) polymorphisms were screened, whereas only primer SCoT44 could stably amplify the specific fragme
APA, Harvard, Vancouver, ISO, and other styles
18

Ciucă, Matilda, Mădălina Butac, Elena Laura Conțescu, Alina Gabriela Turcu, and Mihaela Iordăchescu. "STUDY OF GENETIC DIVERSITY IN PLUM GENOTYPES USING SRAP MARKERS." Fruit Growing Research 38 (December 22, 2022): 70–75. http://dx.doi.org/10.33045/fgr.v38.2022.10.

Full text
Abstract:
The hexaploid European plums (Prunus domestica L. and Prunus domestica subsp. insititia (L.) C.K.Schneid.) are main traditional fruit tree culture in Romania and represent an economically important fruit species with limited information on its genetic structure. Our objective was to fingerprint 33 cultivars using four Sequence-Related Amplified Polymorphism (SRAP) primer pairs to estimate the genetic relationships among local and international cultivars. The primer pairs amplified a total of 70 alleles ranging from 14 to 21 alleles per marker. A wide range of fragment length was detected among
APA, Harvard, Vancouver, ISO, and other styles
19

Wahba, Lydia E., Nor Hazlina, A. Fadelah, and Wickneswari Ratnam. "Genetic Relatedness among Dendrobium (Orchidaceae) Species and Hybrids Using Morphological and AFLP Markers." HortScience 49, no. 5 (2014): 524–30. http://dx.doi.org/10.21273/hortsci.49.5.524.

Full text
Abstract:
Dendrobium is one of the largest genera in the Orchidaceae family. Information on the genetic diversity and relationships among species and hybrids is important for breeding purposes and species conservation. The objectives of this study were to assess genetic relatedness and to determine whether morphological, molecular, or combined analysis can discriminate among Dendrobium species, commercial hybrids, and interspecific hybrids. A total of 81 Dendrobium accessions were characterized with 12 amplified fragment length polymorphism (AFLP) primer pairs and 21 morphological characters. Mean genet
APA, Harvard, Vancouver, ISO, and other styles
20

Kopylov, K. V., and K. V. Kopylova. "STUDY OF NON-CODING AREAS OF THE DNA GENOME OF DIFFERENT ANIMAL SPECIES." Animal Breeding and Genetics 65 (July 3, 2023): 184–90. http://dx.doi.org/10.31073/abg.65.17.

Full text
Abstract:
The introduction to the review article contains information that the genome is the totality of the DNA of a species, that is, the totality of all genes, non-coding regions of nuclear DNA and extrachromosomal genetic material, which includes mitochondrial, plastid DNA, plasmids, etc. An exon is a stretch of DNA within a gene that is translated into a mature matrix RNA (mRNA) molecule by transcription and splicing. They are separated by non-coding sequences (introns), that is, sections of DNA that are part of the gene, but, unlike exons, do not contain information about the amino acid sequence.
APA, Harvard, Vancouver, ISO, and other styles
21

Kon, T., T. Melgarejo, A. Almanzar, and R. L. Gilbertson. "Recent Emergence of the Mild Strain of Tomato yellow leaf curl virus as a Cause of Tomato Yellow Leaf Curl Disease of Processing Tomatoes (Solanum lycopersicon) in the Dominican Republic." Plant Disease 98, no. 11 (2014): 1592. http://dx.doi.org/10.1094/pdis-06-14-0651-pdn.

Full text
Abstract:
In the early 1990s, the monopartite begomovirus Tomato yellow leaf curl virus (TYLCV) was introduced into the Dominican Republic (DO), and molecular characterization revealed it was an isolate of TYLCV-Israel (TYLCV-IL[DO]) (3,5). In 2006, a study of the variability of TYLCV in DO revealed that TYLCV-IL[DO] was associated with all samples of tomato yellow leaf curl (TYLC) tested and, thus, that the virus had been genetically stable for >15 years (2). However, in 2010 and 2011, 2 of 10 and 11 of 18 samples of TYLC, respectively, were negative for TYLCV infection based upon PCR with the TYLCV
APA, Harvard, Vancouver, ISO, and other styles
22

Khrabrova, Liudmila A., Anna A. Nikolaeva, Nina V. Blohina, and Sergey I. Sorokin. "GENETIC DIVERSITY OF MITOCHONDRIAL DNA HAPLOGROUPS IN THE DON HORSE BREED." Siberian Journal of Life Sciences and Agriculture 15, no. 4 (2023): 278–90. http://dx.doi.org/10.12731/2658-6649-2023-15-4-278-290.

Full text
Abstract:
The Don breed of horses, which became famous in Russia already in the middle of the 18th century, was obtained by long-term crossing of nomadic horses with cultured oriental breeds. Due to the reduction in the number of Don queens to 200 broodmares, the research of the genetic characteristics of this breed is of particular relevance. The purpose of the research was to study the matrilineal structure of the Don horse breed based on phylogenetic analysis of 530 nucleotide pairs [n.p.] of the hypervariable section of the D-loop of mitochondrial DNA [mtDNA] in 26 mares representing the main female
APA, Harvard, Vancouver, ISO, and other styles
23

Shih, S. L., S. K. Green, W. S. Tsai, and C. Ssekyewa. "Molecular Characterization of a Begomovirus Associated with Tomato Leaf Curl Disease in Uganda." Plant Disease 90, no. 2 (2006): 246. http://dx.doi.org/10.1094/pd-90-0246a.

Full text
Abstract:
During the summer of 2003, leaf curl symptoms were observed in tomato (Lycopersicon esculentum) plantings in the Iganga District of Uganda. Begomoviral infection was suspected. Twelve symptomatic samples were collected. Begomoviral DNA was extracted and amplified using polymerase chain reaction (PCR) with the begomovirus-specific degenerate primer pair PAL1v1978/PAR1c715 (4). The expected 1.4-kb PCR products were obtained from 11 of 12 samples. The 1.4-kb PCR product of one of the samples was cloned and sequenced. Based on the sequence of the 1.4-kb DNA product, specific primers were designed
APA, Harvard, Vancouver, ISO, and other styles
24

Holeva, M. C., P. E. Glynos, C. D. Karafla, E. M. Koutsioumari, K. B. Simoglou, and E. Eleftheriadis. "First Report of Candidatus Phytoplasma solani Associated with Potato Plants in Greece." Plant Disease 98, no. 12 (2014): 1739. http://dx.doi.org/10.1094/pdis-05-14-0488-pdn.

Full text
Abstract:
In August 2013, potato plants (Solanum tuberosum) cv. Banba displaying symptoms resembling those caused by Candidatus Phytoplasma solani (potato stolbur phytoplasma) were observed in a 2-ha field in the area of the Peripheral Unit of Drama (northern Greece). The plants were 10 weeks old and their symptoms included reddening and upward rolling of leaflets, reduced size of leaves, shortened internodes, and aerial tuber formation. Incidence of affected plants was estimated to be 40% in the field. Four symptomatic potato plants were collected for laboratory testing of possible phytoplasma infectio
APA, Harvard, Vancouver, ISO, and other styles
25

Ahmed, Y., G. Cirvilleri, and A. M. D'Onghia. "First Report of Verticillium Wilt of Mango (Mangifera indica) Caused by Verticillium dahliae in Italy." Plant Disease 98, no. 8 (2014): 1156. http://dx.doi.org/10.1094/pdis-02-14-0130-pdn.

Full text
Abstract:
Mango (Mangifera indica L.) is an important fruit crop in many tropical and subtropical countries. This crop has been recently introduced in Italy, mainly in Sicily (southern Italy), where it proves to be a good commodity for the national market. However, the future of mango cultivation in Sicily is threatened by diverse biotic and abiotic factors, which may limit production and fruit quality. For this reason, an investigation into soilborne diseases of mango was carried out in summer 2009 and spring 2010 (1). During May 2009, typical symptoms of Verticillium wilt were observed in four young m
APA, Harvard, Vancouver, ISO, and other styles
26

Fedorov, Mikhail S., Alexander A. Filippov, Svetlana A. Syrbu, and Mikhail R. Kiselev. "MOLECULAR COMPLEXES BASED ON para-ALKYLSULFONIC ACIDS AND PYRIDINE DERIVATIVES: STRUCTURE AND MESOMORPHIC PROPERTIES." ChemChemTech 67, no. 12 (2024): 64–72. https://doi.org/10.6060/ivkkt.20246712.7094.

Full text
Abstract:
The paper presents the results of theoretical and experimental studies of new mesogenic molecular complexes based on 4-alkylsulfonic acids and 4-pyridyl-4'-n-alkyloxybenzoates. Using quantum chemistry methods (DFT), the structural units of the systems under study, which are hydrogen-bonded complexes, were modeled. The relationship between molecular parameters, electronic effects of substituents, gas-phase acidity and characteristics of the formed H-complexes is shown. Electron-donating alkyl substituents in the para-position of benzenesulfonic acid reduce gas-phase acidity compared to the unsu
APA, Harvard, Vancouver, ISO, and other styles
27

Li, Kangcong, Yan Gao, Xiuqin Li, Yan Zhang, Benfeng Zhu, and Qinghe Zhang. "Fragmentation Pathway of Organophosphorus Flame Retardants by Liquid Chromatography–Orbitrap-Based High-Resolution Mass Spectrometry." Molecules 29, no. 3 (2024): 680. http://dx.doi.org/10.3390/molecules29030680.

Full text
Abstract:
Organophosphorus flame retardants (OPFRs) have been widely used in polymeric materials owing to their flame retardant and plasticizing effects. Investigating the fragmentation pathway of OPFRs is of great necessity for further discovering and identifying novel pollutants using orbitrap-based high-resolution mass spectrometry (HRMS). A total of 25 OPFRs, including alkyl, halogenated, and aromatic types, were analyzed in this study. The fragmentation pathways of the OPFRs were investigated using orbitrap-based HRMS with high-energy collision dissociation (HCD) in positive mode. The major fragmen
APA, Harvard, Vancouver, ISO, and other styles
28

Cajachagua-Torres, Kim N., Sophia M. Blaauwendraad, Hanan El Marroun, et al. "Fetal Exposure to Maternal Smoking and Neonatal Metabolite Profiles." Metabolites 12, no. 11 (2022): 1101. http://dx.doi.org/10.3390/metabo12111101.

Full text
Abstract:
Fetal tobacco exposure has persistent effects on growth and metabolism. The underlying mechanisms of these relationships are yet unknown. We investigated the associations of fetal exposure to maternal smoking with neonatal metabolite profiles. In a population-based cohort study among 828 mother-infant pairs, we assessed maternal tobacco use by questionnaire. Metabolite concentrations of amino acids, non-esterified fatty acids, phospholipids and carnitines were determined by using LC-MS/MS in cord blood samples. Metabolite ratios reflecting metabolic pathways were computed. Compared to non-expo
APA, Harvard, Vancouver, ISO, and other styles
29

Ermolaev, Vadim, Tatiana Gerasimova, Liliya Kadyrgulova, et al. "Ferrocene-Containing Sterically Hindered Phosphonium Salts." Molecules 23, no. 11 (2018): 2773. http://dx.doi.org/10.3390/molecules23112773.

Full text
Abstract:
The synthesis and physical properties of the series of the ferrocenyl-containing sterically hindered phosphonium salts based on di(tert-butyl)ferrocenylphosphine is reported. Analysis of voltamogramms of the obtained compounds revealed some correlations between their structures and electrochemical properties. The elongation of the alkyl chain at the P atom as well as replacement of the Br− anion by [BF4]− shifts the ferrocene/ferrocenium transition of the resulting salts into the positive region. DFT results shows that in the former case, the Br− anion destabilizes the corresponding ion pair,
APA, Harvard, Vancouver, ISO, and other styles
30

Sánchez, Ulises Arellano, Lifang Chen, Jin An Wang, et al. "One-Pot Synthesis of W-TiO2/SiO2 Catalysts for the Photodegradation of p-Nitrophenol." International Journal of Photoenergy 2019 (August 1, 2019): 1–13. http://dx.doi.org/10.1155/2019/5748586.

Full text
Abstract:
Double modifications of TiO2 by doping with WO3 and by dispersing on a SiO2 support were made by the one-pot sol-gel method. Doping with W shifts the TiO2 band gap energy from 3.2 eV to around 3.06 eV. The surface area of the supported W-TiO2/SiO2 material was significantly increased, by approximately 3 times, in comparison to the bare TiO2. The photocatalytic activities of the catalysts were evaluated in the degradation reaction of p-nitrophenol in aqueous solution and basic medium. After 240 min of photodegradation, more than approximately 99% p-nitrophenol could be mineralized with the most
APA, Harvard, Vancouver, ISO, and other styles
31

Velikzhanina, Elena I., Tatiana A. Sashina, Olga V. Morozova, Alexander Yu Kashnikov, Natalia V. Epifanova, and Nadezhda A. Novikova. "Unusual BA222-like strains of <i>Rotavirus A</i> (Sedoreoviridae: <i>Rotavirus: Rotavirus A</i>): molecular and genetic analysis based on all genome segments." Problems of Virology 69, no. 4 (2024): 363–76. http://dx.doi.org/10.36233/0507-4088-254.

Full text
Abstract:
Introduction. Rotavirus infection is the major cause of severe dehydrating diarrhea requiring hospitalization in young children worldwide. Due to their segmented genome, rotaviruses are capable of gene reassortment, which makes the emergence and spread of genetically novel strains possible. The purpose of this study was to search for unusual rotaviruses circulating in Nizhny Novgorod in 2021‒2023 and their molecular genetic characterization based on all genome segments. Materials and methods. Rotavirus-positive stool samples of children were examined by PCR genotyping and electrophoresis in PA
APA, Harvard, Vancouver, ISO, and other styles
32

Fernando, W. G. D., J. X. Zhang, M. Dusabenyagasani, X. W. Guo, H. Ahmed, and B. McCallum. "Genetic Diversity of Gibberella zeae Isolates from Manitoba." Plant Disease 90, no. 10 (2006): 1337–42. http://dx.doi.org/10.1094/pd-90-1337.

Full text
Abstract:
Gibberella zeae (anamorph Fusarium graminearum) causes Fusarium head blight, one of the most important diseases of cereals in the Canadian prairies for the last decade. In 2002, 60 isolates of G. zeae were collected and single spored from naturally infected spikes of wheat from Carman and Winnipeg in Manitoba. These isolates were compared using vegetative compatibility analysis and polymerase chain reaction (PCR)-based sequence related amplified polymorphisms (SRAP). Sixteen vegetative compatibility groups (VCG) were found among the 50 isolates tested. Five VCGs were found in the two locations
APA, Harvard, Vancouver, ISO, and other styles
33

Aminov, R. I., N. Garrigues-Jeanjean, and R. I. Mackie. "Molecular Ecology of Tetracycline Resistance: Development and Validation of Primers for Detection of Tetracycline Resistance Genes Encoding Ribosomal Protection Proteins." Applied and Environmental Microbiology 67, no. 1 (2001): 22–32. http://dx.doi.org/10.1128/aem.67.1.22-32.2001.

Full text
Abstract:
ABSTRACT Phylogenetic analysis of tetracycline resistance genes encoding the ribosomal protection proteins (RPPs) revealed the monophyletic origin of these genes. The most deeply branching class, exemplified bytet and otrA, consisted of genes from the antibiotic-producing organisms Streptomyces rimosus andStreptomyces lividans. With a high degree of confidence, the corresponding genes of the other seven classes (Tet M, Tet S, Tet O, Tet W, Tet Q, Tet T, and TetB P) formed phylogenetically distinct separate clusters. Based on this phylogenetic analysis, a set of PCR primers for detection, retri
APA, Harvard, Vancouver, ISO, and other styles
34

Phuong, Linh Nguyen, Frédéric Fina, Romain Zakrajsek, et al. "Abstract 3697: Cell-free DNA fragmentome dynamics as a biomarker for immune-checkpoint inhibition in advanced carcinoma." Cancer Research 85, no. 8_Supplement_1 (2025): 3697. https://doi.org/10.1158/1538-7445.am2025-3697.

Full text
Abstract:
Background: Immune-checkpoint inhibitors (ICIs) have revolutionized cancer treatment, but a large proportion of patients is resistant to the treatment. Genome-wide cfDNA fragmentation profiles (fragmentome) represent a promising non-invasive approach able to monitor and predict response. Methods: The SChISM (Size cfDNA Immunotherapies Signature Monitoring) enrolled 128 advanced pan-cancer ICI-treated patients. Using Adelis technologies (France), based on the patented BIABooster system, pre- and on-treatment cfDNA size profiles were generated from the plasma, without prior DNA extraction. First
APA, Harvard, Vancouver, ISO, and other styles
35

Hadiati, Sri, Riry Prihatini, and Ellina Mansyah. "Identifikasi Molekuler dan Analisis Kekerabatan Aksesi Nenas Menggunakan Marka RAPD Menunjang Perakitan Varietas Unggul Baru." Jurnal Hortikultura 28, no. 1 (2019): 1. http://dx.doi.org/10.21082/jhort.v28n1.2018.p1-12.

Full text
Abstract:
&lt;p&gt;&lt;strong&gt;(&lt;/strong&gt;&lt;em&gt;&lt;strong&gt;Molecular Identification and Relationships Among Several Pineapple Accessions Using RAPD Marker to Support the Assembling New Varieties&lt;/strong&gt;&lt;/em&gt;&lt;strong&gt;)&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Produksi dan produktivitas nenas dapat ditingkatkan antara lain melalui penggunaan varietas unggul. Dalam perakitan varietas, dibutuhkan informasi hubungan kekerabatan antartetuanya agar diperoleh efek heterosis yang tinggi melalui kegiatan identifikasi secara molekuler. Penelitian bertujuan (1) mengetahui tingkat polimorfism
APA, Harvard, Vancouver, ISO, and other styles
36

Zou, Hesong, Wei Liu, Xiaojuan Wang, et al. "Dynamic monitoring of circulating tumor DNA reveals outcomes and genomic alterations in patients with relapsed or refractory large B-cell lymphoma undergoing CAR T-cell therapy." Journal for ImmunoTherapy of Cancer 12, no. 3 (2024): e008450. http://dx.doi.org/10.1136/jitc-2023-008450.

Full text
Abstract:
BackgroundOver 50% of patients with relapsed or refractory large B-cell lymphoma (r/r LBCL) receiving CD19-targeted chimeric antigen receptor (CAR19) T-cell therapy fail to achieve durable remission. Early identification of relapse or progression remains a significant challenge. In this study, we prospectively investigate the prognostic value of dynamic circulating tumor DNA (ctDNA) and track genetic evolution non-invasively, for the first time in an Asian population of r/r patients undergoing CAR19 T-cell therapy.MethodsLongitudinal plasma samples were prospectively collected both before lymp
APA, Harvard, Vancouver, ISO, and other styles
37

Zhang, Jinxiu, W. G. Dilantha Fernando, and William R. Remphrey. "Genetic Diversity and Structure of the Apiosporina morbosa Populations on Prunus spp." Phytopathology® 95, no. 8 (2005): 859–66. http://dx.doi.org/10.1094/phyto-95-0859.

Full text
Abstract:
Populations of Apiosporina morbosa collected from 15 geographic locations in Canada and the United States and three host species, Prunus virginiana, P. pensylvanica, and P. padus, were evaluated using the sequence-related amplified polymorphism (SRAP) technique to determine their genetic diversity and population differentiation. Extensive diversity was detected in the A. morbosa populations, including 134 isolates from Canada and the United States, regardless of the origin of the population. The number of polymorphic loci varied from 6.9 to 82.8% in the geographic populations, and from 41.4 to
APA, Harvard, Vancouver, ISO, and other styles
38

Zhou, Jing, Lingyu Yang, Xin Chen, et al. "Genome-Wide Identification and Characterization of the NF-YA Gene Family and Its Expression in Response to Different Nitrogen Forms in Populus × canescens." International Journal of Molecular Sciences 23, no. 19 (2022): 11217. http://dx.doi.org/10.3390/ijms231911217.

Full text
Abstract:
The NF-YA gene family is a class of conserved transcription factors that play important roles in plant growth and development and the response to abiotic stress. Poplar is a model organism for studying the rapid growth of woody plants that need to consume many nutrients. However, studies on the response of the NF-YA gene family to nitrogen in woody plants are limited. In this study, we conducted a systematic and comprehensive bioinformatic analysis of the NF-YA gene family based on Populus × canescens genomic data. A total of 13 PcNF-YA genes were identified and mapped to 6 chromosomes. Accord
APA, Harvard, Vancouver, ISO, and other styles
39

Kurniasih, Surti, Rubiyo Rubiyo, Asep Setiawan, Agus Purwantara, and Sudarsono Sudarsono. "ANALISIS KERAGAMAN GENETIK PLASMA NUTFAH KAKAO (Theobroma cacaoL.) BERDASARKANMARKA SSR / Analysis of Genetic Variability Germplasm of Cacao (Theobroma cacaoL.)Basedon SSR Marker." Jurnal Penelitian Tanaman Industri 17, no. 4 (2020): 156. http://dx.doi.org/10.21082/jlittri.v17n4.2011.156-162.

Full text
Abstract:
&lt;p&gt;Microsatellite or simple sequence repeat (SSR) markers have proven to be an excellent tool for cultivar identification, pedigree analysis, and genetic distance evaluations among organisms. The objectives of this research were to characterize cacao collection of Indonesian Coffee and Cacao Research Institute (ICCRI) and to analyze their genetic diversity using SSR markers. In this research, 39 SSR primer pairs were used to amplify genomic DNA of 29 cacao clones. Amplified SSR fragments for each primer pair were scored as individual band and used to determine genetic distance among eval
APA, Harvard, Vancouver, ISO, and other styles
40

Xu, Yuhao, Shun Ma, Bo Cai, et al. "Taxonomic Revision of Ningshan Odd-Scaled Snake, Achalinus ningshanensis (Serpentes, Xenodermidae), with Description of a New Subspecies from Western China." Animals 14, no. 23 (2024): 3425. http://dx.doi.org/10.3390/ani14233425.

Full text
Abstract:
Achalinu ningshanensis (Yang, Huang, Jiang, Burbrink, and Huang, 2022) was first described in Ningshan County, Shaanxi Province, China in 2022, based on seven female specimens. In this study, based on phylogenetic analyses using mitochondrial 12S ribosomal RNA (12S), 16S ribosomal RNA (16S), cytochrome c oxidase subunit 1 (CO1), cytochrome b (cyt b) gene fragments, and morphological examinations of specimens, we revise the taxonomic status of A. ningshanensis, and provide additional data on this species. The molecular phylogeny indicated that A. ningshanensis is nested in a highly supported mo
APA, Harvard, Vancouver, ISO, and other styles
41

Montes-Borrego, M., F. J. Muñoz-Ledesma, R. M. Jiménez-Díaz, and B. B. Landa. "Peronospora arborescens Causes Downy Mildew Disease in Commercial Opium Poppy Crops in France." Plant Disease 92, no. 5 (2008): 834. http://dx.doi.org/10.1094/pdis-92-5-0834b.

Full text
Abstract:
Opium poppy is a strategic crop for the pharmaceutical industry because it is the only source of morphine, codeine, and thebaine alkaloid drugs. Approximately 7,360 ha (average from 2001 through 2007) of opium poppy (Papaver somniferum) are grown annually in France, mainly in the Northern-East (Champagne-Ardenne) and Centre-West (Centre and Poitou-Charentes) regions of the country. This acreage accounts for nearly 5.6% of the legally cultivated opium poppies worldwide. Disease symptoms resembling those of downy mildew (2) have been observed frequently in those opium-poppy-growing areas, especi
APA, Harvard, Vancouver, ISO, and other styles
42

Chitsazi, Rezvan, Phillip W. Gingrich, James P. Long, Chong Wu, and Bissan Al-Lazikani. "Abstract 4460: OpencanSARchem: chemistry registration and standardization pipeline for FAIR integration of bioassay data." Cancer Research 85, no. 8_Supplement_1 (2025): 4460. https://doi.org/10.1158/1538-7445.am2025-4460.

Full text
Abstract:
Abstract Introduction: The rapid growth of medicinal chemistry and bioactivity data has driven AI advancements but inconsistencies in chemical representation across databases make bioactivity-focused data integration challenging. Tautomerism, a key challenge, complicates chemical registration due to the existence of multiple interchangeable molecular forms, significantly impacting database uniqueness and separating bioactivity data across tautomeric forms. We previously developed a standalone, fully deployable solution to address these challenges (Dolciami et al. 2022), now a core component of
APA, Harvard, Vancouver, ISO, and other styles
43

Guniganti, Devendar, and Kumar K. Raj. "DEVELOPMENT OF DOUBLE GENE MAMMALIAN EXPRESSION VECTOR." Biolife 2, no. 3 (2022): 731–37. https://doi.org/10.5281/zenodo.7219727.

Full text
Abstract:
<strong>ABSTRACT</strong> Recombinant vectors are valuable tools in the biopharmaceutical industry with a number of novel vectors being developed every day. These Antibodies are often expressed in mammalian cells by co-transfecting light chain and heavy chain containing plasmids. However, co-transfection can lead to variable in the copy number of both heavy chain and light chain, there by affecting the protein productivity. Double gene expression vectors can overcome these problems. In the current design The first transcriptional unit comprises sequences for the CMV promoter, multiple cloning
APA, Harvard, Vancouver, ISO, and other styles
44

Yang, Chunhai, Huilong Dong, Xue Li, et al. "IGMH‐based research on the intramolecular weak interaction of TKX‐50." Propellants, Explosives, Pyrotechnics, March 7, 2024. http://dx.doi.org/10.1002/prep.202300272.

Full text
Abstract:
AbstractTo further comprehensively study the intramolecular weak interaction of the gaseous TKX‐50 molecule, the two conformations of the TKX‐50 molecule were analyzed via the Independent Gradient Model based on the Hirshfeld Partition (IGMH) method based on the B3LYP/6‐311 g (d,p) level for geometry optimization and for single point energy. The conclusions manifest that the weak interactions between these fragments are mainly composed of hydrogen bonds and van der Waals interactions. From the strength of inter‐fragment interactions formed by contributing atomic pairs and their percentage cont
APA, Harvard, Vancouver, ISO, and other styles
45

Albert, Kireev, Osipenko Sergey, Nikolaev Evgeny, and Kostyukevich Yury. "In silico structures, mass-spectra and retention indices database development for purposes of Chemical Weapons Convention." July 10, 2023. https://doi.org/10.5281/zenodo.8131235.

Full text
Abstract:
<strong>Abstract: </strong>The Chemical Weapons Convention (CWC) is a science-based international treaty for the disarmament and non-proliferation of chemical weapons. However, reference OPCW central analytical database (OCAD) of structures with mass-spectra (MS) and retention indices (RI) includes only minor part of all possible chemical species defined in the Schedules of CWC. In this work we employed OCAD <em>in silico</em> augmentation based on chemoinformatics approach for chemical structures enumeration, MS data generation based on message passing neural network and based on P-alkyl mole
APA, Harvard, Vancouver, ISO, and other styles
46

Kaur, Harleen, Jhulia Gelain, Marcus Vinicius Marin, Natalia A. Peres, and Guido Schnabel. "Development of a molecular tool for identification of a new Neopestalotiopsis sp. associated with disease outbreaks on strawberry." Plant Disease, November 16, 2022. http://dx.doi.org/10.1094/pdis-09-22-2117-re.

Full text
Abstract:
A new Neopestalotiopsis sp. was recently reported causing outbreak of leaf spot and fruit rot on strawberry in Florida, Georgia, and South Carolina. In contrast to other Pestalotiopsis pathogens, the new species appears more aggressive and destructive on strawberry. Current chemical options for management are disease suppressive at best and affected growers have been experiencing major yield losses. In this study, we developed a molecular method based on polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) for identification of the new Neopestalotiopsis sp. from strawb
APA, Harvard, Vancouver, ISO, and other styles
47

Chan, Kin Onn, Stefan T. Hertwig, Dario N. Neokleous, Jana M. Flury, and Rafe M. Brown. "Widely used, short 16S rRNA mitochondrial gene fragments yield poor and erratic results in phylogenetic estimation and species delimitation of amphibians." BMC Ecology and Evolution 22, no. 1 (2022). http://dx.doi.org/10.1186/s12862-022-01994-y.

Full text
Abstract:
Abstract Background The 16S mitochondrial rRNA gene is the most widely sequenced molecular marker in amphibian systematic studies, making it comparable to the universal CO1 barcode that is more commonly used in other animal groups. However, studies employ different primer combinations that target different lengths/regions of the 16S gene ranging from complete gene sequences (~ 1500 bp) to short fragments (~ 500 bp), the latter of which is the most ubiquitously used. Sequences of different lengths are often concatenated, compared, and/or jointly analyzed to infer phylogenetic relationships, est
APA, Harvard, Vancouver, ISO, and other styles
48

Xu, Yanchun, Ying Dong, Yan Deng, et al. "Molecular identification of vivax malaria relapse patients in the Yunnan Province based on homology analysis of the Plasmodium vivax circumsporozoite protein gene." Parasitology Research, November 5, 2022. http://dx.doi.org/10.1007/s00436-022-07700-7.

Full text
Abstract:
AbstractMore than 85% of the malaria burden in the Yunnan Province is caused by imported vivax malaria, and Yunnan is also where the majority of vivax malaria patients are diagnosed in China. Timely removal of the infection sources of Plasmodium vivax and its breeding environment remains the key to eliminating the secondary transmission of imported malaria. To that end, blood samples were collected from cases diagnosed and revalidated as single species infection with P. vivax in the Yunnan Province from 2013 to 2020. Specifically, samples from vivax malaria patients with suspected relapses epi
APA, Harvard, Vancouver, ISO, and other styles
49

Indumathy, R. "NATURE INSPIRED ALGORITHMS TO SOLVE DNA FRAGMENT ASSEMBLY PROBLEM: A SURVEY." June 29, 2012. https://doi.org/10.5121/ijbb.2012.2205.

Full text
Abstract:
International Journal on Bioinformatics &amp; Biosciences (IJBB) Vol.2, No.2, June 2012 DOI : 10.5121/ijbb.2012.2205 45 NATURE INSPIRED ALGORITHMS TO SOLVE DNA FRAGMENT ASSEMBLY PROBLEM: A SURVEY Indumathy R 1 and Uma Maheswari S 2 1Research Scholar, 2Associate Professor Department of Electronics and Communication Engineering, Coimbatore Institute of Technology, Tamilnadu, India 1indumathy.rajagopal@gmail.com 2umamaheswari.cit@gmail.com ABSTRACT Evolutionary search algorithms are becoming an essential advantage in the algorithmic toolbox for solving multi-dimensional optimization problems in a
APA, Harvard, Vancouver, ISO, and other styles
50

Witter, Rute, Andréia Lima Tomé Melo, Thábata Dos Anjos Pacheco, et al. "MOLECULAR DETECTION AND SEROPREVALENCE OF Ehrlichia sp. IN DAIRY CATTLE FROM BRAZIL’S WESTERN AMAZON REGION." Archives of Veterinary Science 26, no. 2 (2021). http://dx.doi.org/10.5380/avs.v26i2.74784.

Full text
Abstract:
Ehrlichia minasensis, a novel species of Ehrlichia that is closely related to E. canis, is known to infect cattle and deer in Canada. This rickettsial bacterium was isolated from Rhipicephalus (Boophilus) microplus ticks and from dairy and beef cattle in southeast and midwestern Brazil, respectively. The purpose of this study was to determine the seroprevalence and perform molecular detection of Ehrlichia sp. in dairy cattle in the northern region of Brazil. The study was conducted in the municipality of Ji-Paraná, located in the state of Rondônia in the western Brazilian Amazon region. Blood
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!