Academic literature on the topic 'Molecular pharming'
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Journal articles on the topic "Molecular pharming"
Paul, Mathew, Craig van Dolleweerd, Pascal M. W. Drake, Rajko Reljic, Harry Thangaraj, Tommaso Barbi, Elena Stylianou, et al. "Molecular pharming." Human Vaccines 7, no. 3 (March 2011): 375–82. http://dx.doi.org/10.4161/hv.7.3.14456.
Full textBoscaiu, Monica, and Oscar Vicente. "Plant ‘molecular pharming’." Journal of Biotechnology 231 (August 2016): S6—S7. http://dx.doi.org/10.1016/j.jbiotec.2016.05.048.
Full textHumphreys, John M., and Clint Chapple. "Molecular ‘pharming’ with plant P450s." Trends in Plant Science 5, no. 7 (July 2000): 271–72. http://dx.doi.org/10.1016/s1360-1385(00)01680-0.
Full textRamessar, Koreen, Teresa Capell, and Paul Christou. "Molecular pharming in cereal crops." Phytochemistry Reviews 7, no. 3 (February 23, 2008): 579–92. http://dx.doi.org/10.1007/s11101-008-9087-3.
Full textMarsian, Johanna, and George P. Lomonossoff. "Molecular pharming — VLPs made in plants." Current Opinion in Biotechnology 37 (February 2016): 201–6. http://dx.doi.org/10.1016/j.copbio.2015.12.007.
Full textHorn, Michael E. "Plant molecular pharming 2012 and beyond." Plant Cell Reports 31, no. 3 (February 4, 2012): 437–38. http://dx.doi.org/10.1007/s00299-012-1227-y.
Full textSpalding, B. J. "Transgenic Pharming Advances." Nature Biotechnology 10, no. 5 (May 1992): 498–99. http://dx.doi.org/10.1038/nbt0592-498.
Full textAbdullah, M. A., Anisa ur Rahmah, A. J. Sinskey, and C. K. Rha. "Cell Engineering and Molecular Pharming for Biopharmaceuticals." Open Medicinal Chemistry Journal 2, no. 1 (May 14, 2008): 49–61. http://dx.doi.org/10.2174/1874104500802010049.
Full textMa, J. K. C. "Molecular pharming—innovation, product development and manufacture." New Biotechnology 25 (September 2009): S282. http://dx.doi.org/10.1016/j.nbt.2009.06.636.
Full textLössl, Andreas G., and Jihong L. Clarke. "Conference Scene: Molecular pharming: manufacturing medicines in plants." Immunotherapy 5, no. 1 (January 2013): 9–12. http://dx.doi.org/10.2217/imt.12.146.
Full textDissertations / Theses on the topic "Molecular pharming"
Pang, Ee Leen. "Molecular pharming of consensus dengue viral envelope glycoprotein domain III in planta and its immunogenicity profiles in BALB/c mice." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/52023/.
Full textVamvaka, Evangelia. "Rice endosperm as a production platform for third-generation HIV microbicides." Doctoral thesis, Universitat de Lleida, 2014. http://hdl.handle.net/10803/285629.
Full textEl objetivo de mi tesis doctoral es la producción en el endospermo de arroz de una tercera generación de microbicidas contra el virus del SIDA. Durante mi trabajo de investigación utilicé el anticuerpo monoclonal 2G12 y dos proteínas que neutralizan el virus, CV-N y GRFT, solas o en combinación, para explorar diferentes estrategias de producción. En la primera parte de mi tesis investigué si podía producir 2G12 en el endospermo de arroz y cuáles eran los factores que afectaban a su producción. En la segunda parte de mi tesis, he introducido GRFT en el endospermo de arroz y he investigado si la proteína producida tenía actividad neutralizante del virus del SIDA. La expresión de GRFT en el endospermo del arroz fue comparable o incluso en algún caso más alta a GRFT producida de forma transitoria en tabaco. En la tercera parte de mi tesis he investigado si el endospermo de arroz sería una plataforma funcional para la producción de CV-N y si esta proteína sería capaz de neutralizar el virus del sida. La proteína Finalmente he utilizado una combinación de microbicidas 2G12, CV-N y GRFT para introducirlos simultáneamente en el endospermo de arroz e identificar la mejor combinación contra el virus del sida, y si la co-expresión potenciaba su efecto neutralizante. La combinación Esta es la primera vez que más de una proteína que neutraliza el virus del sida es producida de forma estable en el endospermo de un cereal, abriendo un nuevo camino hacia la producción de un cóctel microbicida para evitar la transmisión del virus del sida.
The focus of this thesis is the production of third-generation HIV microbicides in rice cells. I used one monoclonal antibody (2G12) and two anti-HIV proteins (CV-N and GRFT) alone or in combination, to explore different strategies to produce an efficient microbicide against HIV. The first part of my PhD thesis was to test whether rice endosperm can function as a platform for the production of 2G12 and to gain insight into the factors that affect the production and functional efficacy of neutralizing antibodies in plants. The human mAb2G12 was expressed successfully into rice endosperms as an aglycosylated form. In the second part of the thesis I introduced GRFT into rice endosperm and I tested whether the rice-derived GRFT protein retains its potent HIV-neutralizing activity. GRFT was successfully expressed in rice endosperm resulting in yields comparable or even higher to GRFT produced transiently in tobacco and other recombinant proteins. In the third part of my thesis I explored whether rice endosperm could function as a production platform to produce CV-N and I evaluated whether the rice-derived CV-N could neutralize efficiently the HIV virus. Finally I used a combinatorial approach introducing the 2G12, CV-N and GRFT into rice cells to identify the best combination against HIV, and whether their co-expression enhanced their effect. This is the first time that more than one protein against HIV is stable expressed into endosperm opening a new way for the production of a microbicide cocktail for the prevention of HIV transmission.
Sabalza, Gallués Maite. "Maize seeds as a production and delivery platform for HIV microbicides." Doctoral thesis, Universitat de Lleida, 2013. http://hdl.handle.net/10803/127189.
Full textEl virus de la inmunodeficiencia humana (VIH) es en la actualidad uno de los problemas de salud pública más graves en el mundo. La aplicación de medidas preventivas para evitar la infección por VIH es la principal vía para reducir la expansión del virus. Los microbicidas son una nueva clase de productos antivirales de bajo coste que podrían ayudar a conseguirlo. Algunos de los obstáculos que pueden frenar el desarrollo de microbicidas son la viabilidad, el aumento de área de cultivo y los altos costes de producción. Se están evaluando muchas plataformas para la producción de microbicidas. Una de las más prometedoras son las plantas, ya que constituyen un sistema económico y seguro. A la producción de proteínas recombinantes farmacéuticas en plantas se le denomina “molecular pharming”. El principal objetivo de esta tesis es el estudio de la producción de moléculas microbicidas tanto a nivel individual (una sola molécula) como combinada (simultaneamente varias moléculas) en semillas de maíz con el fin de desarrollar una nueva estrategia mas económica capaz de evitar la infección por VIH en países en vías de desarrollo. La bioseguridad y los temas relacionados con la legislación relativa a cultivos mejorados genéticamente también son una parte importante de esta tesis, ya que limitan el desarrollo y la comercialización global de este tipo de cultivos.
HIV remains one of the world’s most serious health and development challenges. Preventing HIV infection is one of the strategies that could slow down the spread of the virus. Microbicides are a new class of products that could address this need. However, the feasibility of manufacturing, scalability, and cost are some of the challenges that can undermine the development of cost effective microbicides. One of the most promising production systems is plants because of cost benefits and biological safety. Production of medically important recombinant proteins in plants is known as molecular pharming. The focus of this thesis is the production of microbicide components individually or in combination in maize seeds in order to develop a novel and inexpensive strategy for the prevention of HIV infections in the developing world. I also discuss non-technical barriers to the adoption of GE crops in the European Union and their serious consequences.
Gomes, Carolina Nogueira Carvalho. "Production and analysis of pharmaceutically relevant peptides in Lactuca sativa and Medicago truncatula." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/21655.
Full textO molecular pharming permite a produção de proteinas terapêuticas recombinantes a larga escala, de forma segura e a baixo custo. No presente trabalho, é proposta a produção heteróloga de quatro péptidos inibidores da ACE em dois emergentes sistemas de expressão vegetal, Lactuca sativa (alface) e Medicago truncatula (luz cortada). A utilização da alface, uma planta comestível, pode proporcionar um meio para a administração oral de péptidos anti-hipertensivos, criando um novo alimento funcional. Por outro lado, a utilização de M. truncatula, uma leguminosa modelo, garante não só a facilidade de transformação mas também a extrapolação processual para outras leguminosas. No contexto actual de demanda por terapias alternativas para a hipertensão e de processos mais eficientes de produção de péptidos inibidores da ACE, este trabalho assume particular importância.
Molecular pharming is a cost-effective, scalable and safe system to produce high-quality and biologically active recombinant therapeutic proteins. In the present work the heterologous production of four ACE inhibitory peptides in two emerging plant expression hosts, Lactuca sativa (lettuce) and Medicago truncatula is proposed. The use of lettuce, an edible plant, can provide a means for oral delivery of antihypertensive peptides, thus creating a novel functional food. On another hand, the use of M. truncatula, a model legume, ensures not only the simple transformation process but also the procedural extrapolation to other legume species. In the current scenario of global demand for alternative hypertension therapies and easier ACE inhibitory peptide manufacturing processes, this work assumes particular importance.
Oliveira, Ana Lúcia da Silva. "Molecular pharming in Lactuca sativa L. (Lettuce) : development of protocols." Master's thesis, 2009. http://hdl.handle.net/1822/10869.
Full textAgrobacterium tumefaciens- mediated genetic transformation is an indispensable tool used for the production of genetically modified plants. In our laboratory, we have established a transformation system for lettuce (Lactuca sativa L.) cultivars Batávia Blonde, Butterhead and Queen of May, using Agrobacterium strain EHA 105. Five-day-old mature cotyledon explants was used for transformation study. The selection of transformed shoots was carried out in MS medium fortified with BA (0.1 mg L), NAA (0.1 mg/L) and under the selection of Kanamycin sulphate (50 to BB and 100mg/L to BH and QM) and Ticarcillin (250 mg/L). The transient GUS expression assay was carried out in order to find transformed shoots and to asses the influence of the genotype in the infection process. No differences were found between varieties in the T-DNA transfer rate (100%), but it was observed different behaviours during the regeneration process. Thus, it was shown that the genotype influences the response of different varieties during regeneration but not influences the predisposition to infection by A. tumefaciens. The molecular confirmation by PCR and Southern blot of transformed shoots revealed the foreign gene integration into lettuce genome. The reporter gene show a segregation pattern of Mendel in lettuce, since the generations T1 and T2 had a segregation ration of 3:1. It was proven that the gene gusA has no influence in the phenolic profile of the plants, since no differences were found in the type of compounds produced between transgenic plants and non-transgenic. Having established the protocol for infection of L. sativa L., in an attempt to demonstrate the applicability of this method, it was constructed an expression vector for an animal protein, leukaemia inhibitor factor (LIF), wich has not yet been expressed in plants. The construction of this vector will allow the expression of LIF in plants like lettuce and future studies on LIF yield production and purification in systems like plants. In this way will be possible to compare the different methods to produce LIF and select the ideal.
Transformação mediada por Agrobacterium tumefaciens- é uma ferramenta indispensável na produção de plantas geneticamente modificadas. O presente estudo foi efectuado de forma a estabelecer um sistema de transformação eficiente para diferentes variedades de alface (Batávia Blonde, Butterhead e Queen of May), usando a estirpe de Agrobacterium EHA105. Cotilédones com cinco dias de idade foram usados no estudo de transformação. A selecção dos rebentos caulinares desenvolvidos foi efectuado em meio basal MS suplementado com BA e NAA a 0.1 mg/L e sobre a selecção de canamicina a 50mg/L para Batávia Blonde e 100mg/L para Butterhead e Queen of May e ticarcilina a 250mg/L. A expressão do gene gusA foi realizada para detectar rebentos transformados e verificar a influência do genótipo no processo de infecção. Não foram detectadas diferenças entre as variedades no que diz respeito à percentagem de transferência de T-DNA (100%). Pelo contrário foram detectadas diferenças entre as variedades no processo de regeneração. Isto demonstra que o genótipo exerce influência na resposta das diferentes variedades no processo regenerativo mas não tem influência na prédisposição das plantas para a infecção por Agrobacterium. A confirmação da integração do gene no genoma foi feita por análise de PCR e Southern blot. O gene repórter demonstrou que na alface transformada houve segregação do gene segundo Mendel, já que as gerações T1 e T2 apresentaram segregação 3:1. Foi provado que o gene gusA não exerceu influência no perfil fenólico das plantas já que não foram observadas diferenças significativas entre planas transgénicas e não transgénicas. Uma vez estabelecido um protocolo de infecção de L. sativa L., de forma a demonstrar a aplicabilidade do método, foi construído um vector de expressão para a proteína animal Leukaemia inhibitory factor (LIF), proteína que ainda não foi expressa em plantas. A construção deste vector permitirá a expressão de LIF em plantas como a alface e permitirá a realização de futuros estudos relativamente ao rendimento da produção da proteína e purificação. Desta forma será possível comparar os diferentes métodos para a produção de LIF e seleccionar o ideal.
Oliveira, Filipe Silva Nunes de. "Heterologous production of oligopeptides with antihypertensive effects in Lactuca sativa and Medicago truncatula." Master's thesis, 2018. http://hdl.handle.net/10362/47275.
Full textBooks on the topic "Molecular pharming"
Kermode, Allison R., and Liwen Jiang, eds. Molecular Pharming. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.
Full textKermode, Allison R., and Liwen Jiang. Molecular Pharming: Applications, Challenges and Emerging Areas. Wiley & Sons, Incorporated, John, 2018.
Find full textKermode, Allison R., and Liwen Jiang. Molecular Pharming: Applications, Challenges and Emerging Areas. Wiley & Sons, Incorporated, John, 2018.
Find full textKermode, Allison R., and Liwen Jiang. Molecular Pharming: Applications, Challenges and Emerging Areas. Wiley & Sons, Incorporated, John, 2018.
Find full textBook chapters on the topic "Molecular pharming"
Chen, Qiang, Matthew Dent, and Hugh Mason. "Molecular Pharming." In Molecular Pharming, 231–73. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch10.
Full textSpiegel, Holger, Eva Stöger, Richard M. Twyman, and Johannes F. Buyel. "Current Status and Perspectives of the Molecular Farming Landscape." In Molecular Pharming, 1–23. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch1.
Full textYang, Daichang, Jiquan Ou, Jingni Shi, Zhibin Guo, Bo Shi, and Naghmeh Abiri. "Transgenic Rice for the Production of Recombinant Pharmaceutical Proteins." In Molecular Pharming, 275–307. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch11.
Full textHood, Elizabeth E., and Carole L. Cramer. "Enzymes for Industrial and Pharmaceutical Applications - From Individual to Population Level Impact." In Molecular Pharming, 309–25. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch12.
Full textHundleby, Penny A. C., Markus Sack, and Richard M. Twyman. "Biosafety, Risk Assessment, and Regulation of Molecular Farming." In Molecular Pharming, 327–51. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch13.
Full textTissier, Alain. "Harnessing Plant Trichome Biochemistry for the Production of Useful Compounds." In Molecular Pharming, 353–82. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch14.
Full textFidan, Ozkan, and Jixun Zhan. "Reconstitution of Medicinally Important Plant Natural Products in Microorganisms." In Molecular Pharming, 383–415. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch15.
Full textFeng, Li, and Jingwu Kang. "Screening of Epidermal Growth Factor Receptor Inhibitors in Natural Products Derived From Extracts of Traditional Chinese Medicines." In Molecular Pharming, 417–33. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch16.
Full textLittleton, John, Dustin Brown, Deane Falcone, Gregory Gerhardt, Samir Gunjan, Dennis T. Rogers, and Jatinder Sambi. "Target-Directed Evolution of Mutant Transgenic Plant Cells as a Novel Source of Drugs." In Molecular Pharming, 435–56. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch17.
Full textBasak, Indranil, and Simon G. Møller. "Plant Thermotolerance Proteins, Misfolded Proteins, and Neurodegenerative Diseases." In Molecular Pharming, 457–74. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781118801512.ch18.
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