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Journal articles on the topic 'Molecular probe diagnostic equipment industry'

Author: Grafiati

Published: 4 June 2021

Last updated: 28 July 2025

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1

Zhang, Liding, Xuewei Du, Zhixin Chen, et al. "Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor." International Journal of Molecular Sciences 20, no. 18 (2019): 4645. http://dx.doi.org/10.3390/ijms20184645.

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Salmonella, a common foodborne pathogen, causes many cases of foodborne illness and poses a threat to public health worldwide. Immunological detection systems can be combined with nanoparticles to develop sensitive and portable detection technologies for timely screening of Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system to detect Salmonella. After adding the antibody probe, Salmonella accumulated in the samples on the surfaces of the immuno-NHS beads (INBs), forming a sandwich structure (INB–Salmonella–probes). We demonstra

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Geertsema, Sem, Bernadien H. Jansen, Harry van Goor, Gerard Dijkstra, Klaas Nico Faber, and Arno R. Bourgonje. "Unsuitability of the Oxidation-Reduction Potential Measurement for the Quantification of Fecal Redox Status in Inflammatory Bowel Disease." Biomedicines 11, no. 12 (2023): 3107. http://dx.doi.org/10.3390/biomedicines11123107.

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Oxidative stress is a key pathophysiological process associated with the development and progression of inflammatory bowel disease (IBD). Biomarkers for oxidative stress, however, are scarce, as are diagnostic tools that can interrogate an individual’s gut redox status. This proof-of-concept study aimed to evaluate the potential utility of an oxidation-reduction potential (ORP) measurement probe, to quantify redox status in the feces of both patients with IBD and healthy controls. Previous studies using this ORP measurement probe demonstrated promising data when comparing ORP from severely mal

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Jiménez, Karen Marcela, Dora Janeth Fonseca-Mendoza, Adrien Morel, et al. "Performance Comparison of a Duplex Implementation of the CDC EUA 2019-nCoV Assay with the Seegene Allplex-SARS-CoV-2 Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Swab Samples." Methods and Protocols 5, no. 5 (2022): 73. http://dx.doi.org/10.3390/mps5050073.

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RT-PCR tests have become the gold standard for detecting the SARS-CoV-2 virus in the context of the COVID-19 pandemic. Because of the extreme number of cases in periodic waves of infection, there is a severe financial and logistical strain on diagnostic laboratories. For this reason, alternative implementations and validations of academic protocols that employ the lowest cost and the most widely available equipment and reagents found in different regions are essential. In this study, we report an alternative implementation of the EUA 2019-nCoV CDC assay which uses a previously characterized du

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Sergienko, Vladimir B., and Aleksei A. Ansheles. "Nuclear medicine and molecular imaging in clinical practice: yesterday, today and tomorrow." Terapevticheskii arkhiv 93, no. 4 (2021): 357–62. http://dx.doi.org/10.26442/00403660.2021.04.200673.

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Over the past 40 years, nuclear medicine has grown to be the largest non-invasive diagnostic and therapeutic industry in the world, playing a pivotal role in various fields and disciplines of clinical practice and contributing to improved quality of life and patient prognosis. Over the first 20 years of the XXI century, the number of radionuclide procedures in the world has increased significantly, primarily due to innovations in radiopharmaceuticals, continuous improvement of the technical properties of equipment and the expansion of the boundaries of multimodal imaging. The review examines t

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Lei, Lei, Fan Liao, Lei Tan, et al. "LAMP Coupled CRISPR-Cas12a Module for Rapid, Sensitive and Visual Detection of Porcine Circovirus 2." Animals 12, no. 18 (2022): 2413. http://dx.doi.org/10.3390/ani12182413.

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Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus-associated disease (PCVAD), which can cause considerable economic loss to the pig industry. The diagnosis of PCVAD is complicated and requires a series of clinical, pathological, and virological methods. Therefore, a rapid, highly sensitive, on-site, and visual diagnostic approach would facilitate dealing with the spread of PCV2. In this study, we intended to establish a new and effective PCV2 detection method through combining the no specific equipment requirement advantage of loop-mediated isothermal amplification (LAMP)

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Vaz, Sofia C., Francisco Oliveira, Ken Herrmann, and Patrick Veit-Haibach. "Nuclear medicine and molecular imaging advances in the 21st century." British Journal of Radiology 93, no. 1110 (2020): 20200095. http://dx.doi.org/10.1259/bjr.20200095.

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Currently, Nuclear Medicine has a clearly defined role in clinical practice due to its usefulness in many medical disciplines. It provides relevant diagnostic and therapeutic options leading to patients' healthcare and quality of life improvement. During the first two decades of the 21stt century, the number of Nuclear Medicine procedures increased considerably. Clinical and research advances in Nuclear Medicine and Molecular Imaging have been based on developments in radiopharmaceuticals and equipment, namely, the introduction of multimodality imaging. In addition, new therapeutic application

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Chandu, Dilip, Sudakshina Paul, Mathew Parker, et al. "Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples." BioMed Research International 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/3145921.

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Testing for the presence of genetically modified material in seed samples is of critical importance for all stakeholders in the agricultural industry, including growers, seed manufacturers, and regulatory bodies. While rapid antibody-based testing for the transgenic protein has fulfilled this need in the past, the introduction of new variants of a given transgene demands new diagnostic regimen that allows distinguishing different traits at the nucleic acid level. Although such molecular tests can be performed by PCR in the laboratory, their requirement for expensive equipment and sophisticated

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Kim, Sang-Heon, Jae-Woo Choi, A.-Ru Kim, Sang-Choon Lee, and Moon-Young Yoon. "Development of ssDNA Aptamers for Diagnosis and Inhibition of the Highly Pathogenic Avian Influenza Virus Subtype H5N1." Biomolecules 10, no. 8 (2020): 1116. http://dx.doi.org/10.3390/biom10081116.

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Avian influenza (AI) has severely affected the poultry industry worldwide and has caused the deaths of millions of birds. Highly pathogenic avian influenza virus is characterized by high mortality and the ability to transmit from birds to humans. Early diagnosis is difficult because of the variation in pathogenicity and the genetic diversity between virus subtypes. Therefore, development of a sensitive and accurate diagnostic system is an urgent priority. We developed ssDNA aptamer probes to detect AI viruses. Through seven rounds of SELEX to search for a probe specific to the highly pathogeni

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Eftekhari-Zadeh, Ehsan, Abdallah S. Bensalama, Gholam Hossein Roshani, Ahmed S. Salama, Christian Spielmann, and Abdullah M. Iliyasu. "Enhanced Gamma-Ray Attenuation-Based Detection System Using an Artificial Neural Network." Photonics 9, no. 6 (2022): 382. http://dx.doi.org/10.3390/photonics9060382.

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Scale deposition is the accumulation of various materials in the walls of transmission lines and unwanted parts in the oil and gas production system. It is a leading moot point in all transmission lines, tanks, and petroleum equipment. Scale deposition leads to drastic detrimental problems, reduced permeability, pressure and production losses, and direct financial losses due to the failure of some equipment. The accumulation of oil and gas leads to clogged pores and obstruction of fluid flow. Considering the passage of a two-phase flow, our study determines the thickness of the scale, and the

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Qi, Yong, Wei Li, Xiaoling Li, et al. "Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test." BioMed Research International 2021 (October 4, 2021): 1–13. http://dx.doi.org/10.1155/2021/9957747.

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Objectives. Human adenoviruses (HAdV) are classified as 7 HAdV species, and some serotypes in species B like HAdV 3, HAdV 7, HAdV 21, and HAdV 55 caused severe symptoms, even fatalities. Patients may be misdiagnosed and inadequately treated without reliable and practical methods for HAdV serotyping. Developing rapid, sensitive, and specific diagnostic methods for HAdV is critical. Methods. Detection methods were established based on a recombinase polymerase amplification (RPA) assay and lateral flow (LF) test. Specific target sequence was screened, targeting which, primers and probes were desi

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Liam, C.-K., P. Lee, C.-J. Yu, C. Bai, and K. Yasufuku. "The diagnosis of lung cancer in the era of interventional pulmonology." International Journal of Tuberculosis and Lung Disease 25, no. 1 (2021): 6–15. http://dx.doi.org/10.5588/ijtld.20.0588.

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Advances in bronchoscopic and other interventional pulmonology technologies have expanded the sampling procedures pulmonologist can use to diagnose lung cancer and accurately stage the mediastinum. Among the modalities available to the interventional pulmonologist are endobronchial ultrasound-guided transbronchial needles aspiration (EBUS-TBNA) and transoesophageal bronchoscopic ultrasound-guided fine-needle aspiration (EUS-B-FNA) for sampling peribronchial/perioesophageal central lesions and for mediastinal lymph node staging, as well as navigational bronchoscopy and radial probe endobronchia

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Knox, Alexandra, and Travis Beddoe. "Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens." Animals 11, no. 7 (2021): 2150. http://dx.doi.org/10.3390/ani11072150.

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The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current s

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Bello, Muhammad Bashir, Khatijah Yusoff, Aini Ideris, Mohd Hair-Bejo, Ben P. H. Peeters, and Abdul Rahman Omar. "Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives." BioMed Research International 2018 (August 5, 2018): 1–18. http://dx.doi.org/10.1155/2018/7278459.

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Newcastle disease (ND) is one of the most devastating diseases that considerably cripple the global poultry industry. Because of its enormous socioeconomic importance and potential to rapidly spread to naïve birds in the vicinity, ND is included among the list of avian diseases that must be notified to the OIE immediately upon recognition. Currently, virus isolation followed by its serological or molecular identification is regarded as the gold standard method of ND diagnosis. However, this method is generally slow and requires specialised laboratory with biosafety containment facilities, maki

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Golender, Natalia, Eyal Klement, and Bernd Hoffmann. "Development of New Probe-Based Real-Time RT-qPCR Assays for the Detection of All Known Strains of Bovine Ephemeral Fever Viruses." Viruses 17, no. 3 (2025): 407. https://doi.org/10.3390/v17030407.

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Bovine ephemeral fever is an arthropod-borne viral disease that affects cattle and buffalo in many regions of the world; it causes heavy economic losses in the cattle industry. To date, all BEFV-specific diagnostic molecular assays have been based on the variable glycoprotein (G-protein)-coding genome region, potentially allowing the pathogen to escape detection. We developed two new assays, based on the less variable nucleoprotein genome region, and compared them with two G-protein-based assays. For this comparison, we used 245 samples comprising positive and negative field samples from Israe

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Yang, Hongjun, Herui Wang, Xueyu Sang, Zhengping Zhuang, Andrew Y. Fu, and Michael Sha. "Xenonucleic acid technology in high-sensitivity IDH1/2 mutation detection for glioma molecular profiling." Journal of Clinical Oncology 41, no. 16_suppl (2023): e14052-e14052. http://dx.doi.org/10.1200/jco.2023.41.16_suppl.e14052.

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e14052 Background: Glioma is a life-threatening primary brain tumor that poses significant challenges for diagnosis and treatment. Certain genetic features, such as the IDH1/2 mutation status (codon 132 of IDH1 and codon 172 of IDH2), can help distinguish between different types of gliomas. IDH1/2 wild-type and mutant gliomas have different prognoses and treatment options, with patients with IDH1/2 mutations typically having a better prognosis and a better response to chemotherapy. However, current available molecular tests for IDH1/2 mutation detection have limited sensitivity, and their appl

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Quick, V., M. Hughes, and C. H. Chan. "SAT0271 THE “GOLD STANDARD” DIAGNOSTIC TEST FOR GCA IS THE WHOLE GCA FAST TRACK PATHWAY COMBINED." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 1079–80. http://dx.doi.org/10.1136/annrheumdis-2020-eular.2038.

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Background:We have been developing a rheumatologist-led ultrasound driven giant cell arteritis (GCA) fast-track pathway (FTP), which in year 3 had the following structure:1.Rapid access to rheumatology assessment (RAS) to establish clinical probability of GCA (CP-GCA). No referral criteria required2.Temporal and axillary artery ultrasound (TAUS) if GCA not excluded with RAS. TAUS considered positive if bilateral (halo score ≥2/81,2at >1 temporal artery)3.Second test in selected patients:GCA diagnosed in those with mod-high CP-GCA and +ve TAUS and excluded in those with low CP-GCA and -ve TA

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Luo, Yifan, Chen Gao, Wujie Chen, Kefeng Zhou, and Maosheng Xu. "Molecular Magnetic Resonance Imaging with Contrast Agents for Assessment of Inflammatory Bowel Disease: A Systematic Review." Contrast Media & Molecular Imaging 2020 (May 6, 2020): 1–9. http://dx.doi.org/10.1155/2020/4764985.

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Background and Aims. Magnetic resonance imaging (MRI) has taken an important role in the diagnosis of inflammatory bowel diseases (IBD). In the wake of current advances in nanotechnology, the drug delivery industry has seen a surge of nanoparticles advertising high specificity in target imaging. Given the rapid development of the field, this review has assembled related articles to explore whether molecular contrast agents can improve the diagnostic capability on gastrointestinal imaging, especially for IBD. Methods. Relevant articles published between 1998 and 2018 from a literature search of

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Kaiser, Martin F., Dil Begum, Paula Proszek, et al. "A Rapid and Robust Molecular Diagnostic Approach For Multiple Myeloma – Results From a Large Trial Cohort." Blood 122, no. 21 (2013): 3097. http://dx.doi.org/10.1182/blood.v122.21.3097.3097.

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Abstract Introduction Obtaining reliable information about the molecular subtype of myeloma is and will become ever more important in a number of clinical settings, such as alternative treatment strategies for high risk or ultra high risk disease (Boyd KD et al., Leukemia 2011), or patient selection for small molecule inhibitors, that are currently under development, targeting myeloma subtype specific proteins (e.g. MMSET or MAF). We report here our experience with a novel, highly applicable and high throughput diagnostic approach in a large sample set of 1016 myeloma presentation cases, using

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Michalíková, Monika, Teodor Tóth, Viktória Rajťúková, and Jozef Živčák. "The Digital Pre-Operative Planning of Hip Surgical Interventions." Solid State Phenomena 199 (March 2013): 350–55. http://dx.doi.org/10.4028/www.scientific.net/ssp.199.350.

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Computer technology has many applications in different fields of industry, health care and medicine. This encompasses paper-based information processing as well as data processing machines (Hospital information system or Clinical information system) and image digitalization of a large variety of medical diagnostic equipment (e.g. computer images of X-ray, MR, CT). The aim of the computer technology in medicine is to achieve the best possible support of patient care, preoperative surgery planning and administration by electronic data processing. At the present time in many countries of the worl

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Kaiser, Martin F., Paula Proszek, Dil Begum, et al. "Rapid, Robust and Accessible Molecular Profiling of Biologically and Clinically Relevant Copy Number Alterations in Multiple Myeloma from Small Amounts of Tumor DNA." Blood 124, no. 21 (2014): 5168. http://dx.doi.org/10.1182/blood.v124.21.5168.5168.

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Abstract Background Structural aberrations like copy number alterations (CNA) and translocations play a central role in multiple myeloma tumor biology. The analysis of CNA for complete molecular profiling of myeloma retains clinical significance in the context of recent next-generation sequencing (NGS) results which highlight the highly heterogeneous landscape of single nucleotide mutations, which are often sub-clonal. Detection of CNAs remains the domain of array-based technologies, as demonstrated by the use of copy number arrays by the TCGA consortium and others for CNA detection in NGS pro

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Kaiser, Martin F., Eileen M. Boyle, Brian A. Walker, et al. "High Throughput Genetic Profiling Using a Robust All-Molecular Diagnostic Approach Is Feasible to Specifically Identify a High Risk Group of Myeloma Patients." Blood 124, no. 21 (2014): 172. http://dx.doi.org/10.1182/blood.v124.21.172.172.

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Abstract Introduction Co-segregation of two or more adverse structural genetic aberrations in myeloma is associated with a particularly bad outcome and defines a molecular high risk subgroup of patients that is in urgent need of innovative treatment approaches (Boyd, Leukemia 2012). Interphase in situ fluorescence hybridization(iFISH) is the current clinical standard for detecting structural genetic aberrations in myeloma. However, iFISH is labor-intensive, slow and dependent on investigator expertise, which makes standardization difficult. There is an urgent need to develop a standardized and

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Kotrashetti, Vijayalakshmi Sharadindu, Kishore Gajanan Bhat, Vijay Mahadev Kumbar, and Sachita Shrikant Naik. "Isolation, identification, and quantification of Prevotella intermedia and Prevotella nigrescens in subgingival plaque samples of periodontally healthy and chronic periodontitis patients through chromogenic in situ hybridization." Journal of Indian Society of Periodontology 28, no. 4 (2024): 443–48. https://doi.org/10.4103/jisp.jisp_99_23.

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Background: The genus Prevotella comprising over 50 identified species is a normal commensal of the oral cavity. The commonly studied species are Prevotella intermedia (PI) and Prevotella nigrescens (PN). The molecular technique, used for isolating and identifying these species, requires specialized equipment, making them not feasible in a standard laboratory. Chromogenic in situ hybridization (CISH) can be performed in routine laboratory and is economical and less time-consuming. Hence, the aim of this study was to isolate, identify, and quantify PI and PN from subgingival plaque samples of c

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Minnucci, Giulia, Giulia Amicarelli, Silvia Salmoiraghi, Orietta Spinelli, Daniel Adlerstein, and Alessandro Rambaldi. "A Novel, Highly Sensitive, Rapid, Non-PCR Based Method for the Detection of the JAK2 V617F Mutation in Chronic Myeloproliferative Neoplasms." Blood 114, no. 22 (2009): 3892. http://dx.doi.org/10.1182/blood.v114.22.3892.3892.

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Abstract Abstract 3892 Poster Board III-828 Background The point mutation G1849T (V617F) of the JAK2 gene occurs at high frequency in several Ph-negative chronic myeloproliferative neoplasms (Ph-neg-CMNs), such as Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Myelofibrosis (MF). The molecular analysis of this mutation is mandatory in the diagnostic work up of these diseases. Several molecular diagnostic techniques are currently used but each of them present some important limitations such as a low sensitivity and the requirement of labor intensive procedures performed with expensi

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Honnens, Ä., C. Klein, K. G. Hadeler, H. Niemann, H. Bollwein, and C. Wrenzycki. "295 DOPPLER SONOGRAPHY OF THE OVARIAN ARTERIES IN SUPEROVULATED CATTLE." Reproduction, Fertility and Development 21, no. 1 (2009): 244. http://dx.doi.org/10.1071/rdv21n1ab295.

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Despite considerable progress in the understanding of ovarian follicular growth in cattle, the variable and unpredictable superovulatory response of donor animals is still a limiting factor to the success in the embryo transfer industry. One of the main factors affecting the outcome is the presence or absence of a dominant follicle at the time of gonadotropin treatment. It has been shown that the removal of the dominant follicle using ultrasound-guided aspiration before superovulation increases the number of ova and transferable embryos (Bungartz and Niemann 1994 J. Reprod. Fertil. 101, 581–59

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Kaiser, Martin F., Eileen Mary Boyle, Brian A. Walker, et al. "Specific Identification of High Risk Disease Using Molecular Profiling By Mymap (Myeloma MLPA and translocation PCR) of 1,036 Cases." Blood 126, no. 23 (2015): 2981. http://dx.doi.org/10.1182/blood.v126.23.2981.2981.

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Abstract Introduction Identifying molecular high risk myeloma remains a diagnostic challenge. We previously reported co-segregation of >1 adverse lesion [t(4;14), t(14;16), t(14;20), gain(1q), del(17p)] by iFISH to specifically characterise a group of high risk patients (Boyd et al., Leukemia 2012). However, implementation of this approach is difficult using FISH because of its technical limitations. We recently developed and validated a novel high-throughput all-molecular testing strategy against FISH (MyMaP- Myeloma MLPA and translocation PCR; Kaiser MF et al., Leukemia 2013; Boyle EM et

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Nonaka, Carolina Kymie Vasques, Antônio Augusto Fonseca Junior, Estefânia Oliveira Guedes, et al. "Different methods of real-time PCR for detection of pseudorabies virus." Ciência Rural 47, no. 3 (2017). http://dx.doi.org/10.1590/0103-8478cr20160342.

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ABSTRACT: Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two equipment (ABI7500, Rotorgene 3000) were evaluated. Oligonucleotides targeting gB using hydrolysis probes showed the best performance after evaluating efficiency (99%), the detection limit

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Weng, Shaoting, Shengming Ma, Yueteng Xing, et al. "Toward establishing a rapid constant temperature detection method for canine parvovirus based on endonuclease activities." Microbiology Spectrum, September 17, 2024. http://dx.doi.org/10.1128/spectrum.04222-23.

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ABSTRACT Canine parvovirus (CPV) can cause high morbidity and mortality rates in puppies, posing a significant threat to both pet dogs and the breeding industry. Rapid, accurate, and convenient detection methods are important for the early intervention and treatment of canine parvovirus. In this study, we propose a visual CPV detection system called nucleic acid mismatch enzyme digestion (NMED). This system combines loop-mediated isothermal amplification (LAMP), endonuclease for gene mismatch detection, and colloidal gold lateral chromatography. We demonstrated that NMED can induce the binding

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Isabel, Sandra, Mariana Abdulnoor, Karel Boissinot, et al. "Emergence of a mutation in the nucleocapsid gene of SARS-CoV-2 interferes with PCR detection in Canada." Scientific Reports 12, no. 1 (2022). http://dx.doi.org/10.1038/s41598-022-13995-4.

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AbstractThe emergence of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) was met with rapid development of robust molecular-based detection assays. Many SARS-CoV-2 molecular tests target multiple genetic regions of the virus to maximize detection and protect against diagnostic escape. Despite the relatively moderate mutational rate of SARS-CoV-2, numerous mutations with known negative impact on diagnostic assays have been identified. In early 2021, we identified four samples positive for SARS-CoV-2 with a nucleocapsid (N) gene drop out on Cepheid Xpert® Xpress SARS-CoV-2 assay. Sequ

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Stackhouse, Tammy, Sarah Boggess, Denita Hadziabdic, Robert N. Trigiano, Matthew Ginzel, and William Klingeman. "Conventional Gel Electrophoresis and TaqMan Probes Enable Rapid Confirmation of Thousand Cankers Disease from Diagnostic Samples." Plant Disease, February 16, 2021. http://dx.doi.org/10.1094/pdis-10-20-2258-re.

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Thousand cankers disease (TCD) is caused by the fungal pathogen, Geosmithia morbida,a, and vectored by the walnut twig beetle, Pityophthorus juglandis. In infected walnut and butternut (Juglans spp.) hosts, tree decline and death results in ecological disruption and economic losses. A rapid molecular detection protocol for TCD using microsatellite markers can confirm the presence of insect vector or fungal pathogen DNA, but it requires specialized expensive equipment, and technical expertise. Using four different experimental approaches, capillary and conventional gel electrophoresis, and trad

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"Industry Watch." Asia-Pacific Biotech News 12, no. 12 (2008): 38–66. http://dx.doi.org/10.1142/s0219030308000773.

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Stem Cell Sciences Announces the Creation of the World's First Authentic Rat Embryonic Stem Cells from Its Exclusively Licensed Technology QIAGEN Unveils Diagnostic Assay in the EU to Detect Genetic Variation Causing Adverse Reactions in AIDS Patients QIAGEN and Chinese Academy of Sciences Form Collaboration for Development of New Molecular Food Safety Tests Agilent Technologies Creates Specialized Training Program in China for Melamine Analysis in Milk, Milk Products Sinovac Initiates Development of Vaccine against HFMD Varian Medical Systems Signs Collaboration Agreement to Bring Advanced Ra

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Li, Changfeng, Yuliang Ju, Xun Wu, et al. "Development of recombinase polymerase amplification combined with lateral flow detection assay for rapid and visual detection of Ralstonia solanacearum in tobacco." Plant Disease, July 8, 2021. http://dx.doi.org/10.1094/pdis-04-21-0688-re.

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Bacterial wilt caused by Ralstonia solanacearum is a serious soil-borne disease that results in severe losses to tobacco (Nicotiana tabacum) production in China. In this study, a novel RPA-LFD assay for the rapid visual detection of R. solanacearum was established using recombinase polymerase amplification (RPA) and lateral-flow dipstick (LFD). The RPA-LFD assay was performed at 37°C in 30 min without complex equipment. Targeting the sequence of the RipTALI-9 gene, we designed RPA primers (Rs-rpa-F/R) and an LF probe (Rs-LF-probe) that showed high specificity to R. solanacearum. The sensitivit

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Menezes Gama, Rodolpho Guilherme, Aline de Souza Ramos, and Ana Claudia Fernandes Amaral. "A Diagnostic Tool for Good Chromatographic Practices Applied to HPLC in Pharmaceutical Quality Control." Current Pharmaceutical Analysis 19 (August 23, 2023). http://dx.doi.org/10.2174/1573412919666230823140503.

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Background: High-performance liquid chromatography is one of the most used analytical techniques in quality control in the pharmaceutical industry. Since it is a complex technique, it needs good practices that can contribute to compliance with regulatory requirements. Objetive: This study aims to establish a diagnostic tool for Good Chromatographic Practices (GCP) for the self-assessment of a Quality Control Laboratory (QCL). Methods: The research was carried out on scientific bases, pharmaceutical legislation, as well as guides published by manufacturers. Results: Seven axes of action were id

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Kaur, Harleen, Raghuwinder Singh, Vinson P. Doyle, and Rodrigo Valverde. "A Diagnostic TaqMan Real-Time PCR Assay for in planta Detection and Quantification of Colletotrichum theobromicola, Causal Agent of Boxwood Dieback." Plant Disease, February 25, 2021. http://dx.doi.org/10.1094/pdis-11-20-2439-re.

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Boxwood dieback, caused by Colletotrichum theobromicola, is spreading at an alarming rate in the boxwood industry in the United States. Although C. theobromicola has been accepted as a distinct species within the C. gloeosporioides species complex, it is difficult to distinguish it from other closely related species based on morphology. Moreover, molecular identification of C. theobromicola requires amplification and sequencing of multiple loci, which can be expensive and time consuming. Therefore, a diagnostic TaqMan real-time PCR assay was developed for early and accurate detection and quant

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Zhao, Mengdi, Xizhen Wang, Kun Wang, et al. "Recombinant polymerase amplification combined with lateral flow strips for the detection of deep-seated Candida krusei infections." Frontiers in Cellular and Infection Microbiology 12 (July 29, 2022). http://dx.doi.org/10.3389/fcimb.2022.958858.

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The incidence of Candida infections in intensive care units (ICU) has significantly increased in recent years, and these infections have become one of the most serious complications threatening the lives of ICU patients. The proportion of non-Candida albicans infections, such as Candida krusei and Candida glabrata infections, which are resistant to fluconazole, is increasing each year. Early identification of the strains causing Candida infections is important for the timely implementation of targeted treatments to save patients’ lives. However, the current methods of direct microscopy, cultur

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Zhao, Yu, Changyu Zhou, Boyan Guo, Xin Yang, and Hongning Wang. "Pyrococcus furiosus Argonaute-mediated porcine epidemic diarrhea virus detection." Applied Microbiology and Biotechnology 108, no. 1 (2024). http://dx.doi.org/10.1007/s00253-023-12919-0.

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Abstract Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, induces severe vomiting and acute watery diarrhea in unweaned piglets. The pig industry has suffered tremendous financial losses due to the high mortality rate of piglets caused by PEDV. Consequently, a simple and rapid on-site diagnostic technology is crucial for preventing and controlling PEDV. This study established a detection method for PEDV using recombinase-aided amplification (RAA) and Pyrococcus furiosus Argonaute (PfAgo), which can detect 100 copies of PEDV without cross-reactivity with other pathogens. The enti

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Quintela, Irwin A., Tyler Vasse, Chih-Sheng Lin, and Vivian C. H. Wu. "Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens." Frontiers in Microbiology 13 (December 5, 2022). http://dx.doi.org/10.3389/fmicb.2022.1054782.

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Traditional foodborne pathogen detection methods are highly dependent on pre-treatment of samples and selective microbiological plating to reliably screen target microorganisms. Inherent limitations of conventional methods include longer turnaround time and high costs, use of bulky equipment, and the need for trained staff in centralized laboratory settings. Researchers have developed stable, reliable, sensitive, and selective, rapid foodborne pathogens detection assays to work around these limitations. Recent advances in rapid diagnostic technologies have shifted to on-site testing, which off

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Bohorquez, Jose Alejandro, Saraswathi Lanka, Rosa Rosell, et al. "Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method." Frontiers in Cellular and Infection Microbiology 13 (January 27, 2023). http://dx.doi.org/10.3389/fcimb.2023.1114772.

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African swine fever virus (ASFV) currently represents the biggest threat to the porcine industry worldwide, with high economic impact and severe animal health and welfare concerns. Outbreaks have occurred in Europe and Asia since ASFV was reintroduced into the continent in 2007 and, in 2021, ASFV was detected in the Caribbean, raising alarm about the reemergence of the virus in the Americas. Given the lack of vaccines against ASFV, control of the virus relies on molecular surveillance, which can be delayed due to the need for sample shipment to specialized laboratories. Isothermal PCR techniqu

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Wang, Fang, Yan Wang, Xia Liu, et al. "Rapid, Simple, and Highly Specific Detection of Streptococcus pneumoniae With Visualized Recombinase Polymerase Amplification." Frontiers in Cellular and Infection Microbiology 12 (June 2, 2022). http://dx.doi.org/10.3389/fcimb.2022.878881.

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Streptococcus pneumoniae is a major pathogen that causes microbiological illness in humans. The introduction of polyvalent vaccines has resulted in a significant decrease in pneumococcal-related mortality. However, pneumococcal infections continue to be a leading cause of death in children under the age of 5 and adults over the age of 65 worldwide. A speedy and highly sensitive diagnostic tool is necessary for routine adoption to adequately manage patients and control the spread of infection. In this study, we investigated a new nucleic acid amplification technique, isothermal recombinase poly

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Ge, Duobao, Fang Wang, Yanyan Hu, Bendi Wang, Xuzhu Gao, and Zhenxing Chen. "Fast, Simple, and Highly Specific Molecular Detection of Porphyromonas gingivalis Using Isothermal Amplification and Lateral Flow Strip Methods." Frontiers in Cellular and Infection Microbiology 12 (May 25, 2022). http://dx.doi.org/10.3389/fcimb.2022.895261.

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Porphyromonas gingivalis is an important oral pathogen that causes periodontal disease and is difficult to culture under conventional conditions. Therefore, a reliable technique for detecting this pathogenic bacterium is required. Here, isothermal recombinase polymerase amplification (RPA), a new nucleic acid amplification method, was combined with a visualization method based on nanoparticle-based lateral flow strips (LFS) for the rapid detection of P. gingivalis. The species-specific 16S rRNA sequence of P. gingivalis was used as the target for RPA, and a set of specific primer–probe combina

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Chakraborty, Moutoshi, Shamsul Arafin Bhuiyan, Simon Strachan, Muhammad J. A. Shiddiky, Nam-Trung Nguyen, and Rebecca Ford. "Development and validation of a LAMP-based method for rapid and reliable detection of Xanthomonas albilineans, the causal agent of sugarcane leaf scald." Frontiers in Microbiology 16 (January 30, 2025). https://doi.org/10.3389/fmicb.2025.1537812.

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IntroductionXanthomonas albilineans (Xalb)-induced leaf scald (LS) is a significant bacterial disease affecting sugarcane and posing a global threat to the sugarcane industry. The presence of irregular symptoms makes traditional phenotypic detection difficult, and molecular methods necessitate costly equipment, labor, and extended sample-to-answer processing times.MethodsThis study introduces an innovative rapid DNA isolation method requiring no reagents, combined with an isothermal amplification-based assay for efficient detection of Xalb DNA in sugarcane xylem sap, leaf tissue, and meristema

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Li, Weisong, Bo Cai, Ranran Chen, Jianchen Cui, Hui Wang, and Zhihong Li. "Application of Recombinase Polymerase Amplification with CRISPR/Cas12a and Multienzyme lsothermal Rapid Amplification with lateral flow dipstick assay for Bactrocera correcta." Pest Management Science, February 20, 2024. http://dx.doi.org/10.1002/ps.8035.

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AbstractBACKGROUNDBactrocera correcta is a quarantine pest that negatively impacts the fruit and vegetable industry. Differentiating B. correcta from similar species, especially in non‐adult stages, remains challenging. Rapid molecular identification techniques, such as Recombinase Polymerase Amplification (RPA) combined with CRISPR/Cas12a and Multienzyme lsothermal Rapid Amplification with lateral flow dipstick (MIRA‐LFD), play a crucial role in early monitoring and safeguarding agricultural production. Our study introduces two methods for the rapid visual identification of B. correcta.RESULT

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"Bioboard." Asia-Pacific Biotech News 12, no. 12 (2008): 5–18. http://dx.doi.org/10.1142/s021903030800075x.

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AUSTRALIA — Prototype Test for Predicting Clinical Outcome for Melanoma Patients AUSTRALIA — New Model Predicts Long-term Survival of Critically Ill Patients AUSTRALIA — World's First Computerized System to Predict Premature Births CHINA — Biomagnetics (BMGP) Enters Chinese Biotech Market CHINA — More Than 12 000 Babies Hospitalized for Taking Tainted Milk CHINA — New Test Could Prevent Cervical Cancer INDONESIA — Indonesia's Pharmaceutical Companies Get Boost in Competitive Advantage from Launch of Newport Horizon Premium and IDRAC Solutions INDIA — India's Government Nods to Set up 10 Testin

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Bel Hadj Ali, Insaf, Yusr Saadi-Ben Aoun, Imen Khammeri, et al. "Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis patients." Frontiers in Microbiology 15 (January 27, 2025). https://doi.org/10.3389/fmicb.2024.1514684.

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IntroductionCutaneous leishmaniases (CL), a wide range of cutaneous diseases caused by diverse species of Leishmania genus parasites, are among the most neglected infectious diseases. While they are non-fatal, CL are highly morbid with disfiguring lesions, which could be chronic, leaving lifelong unsightly scars; they are combined with psychological distress and social stigma. The efficiency of treatment highly depends on the infecting Leishmania species. Diagnosis is mainly based on microscopic direct examination (DE) of Giemsa-stained smears needing experienced microscopists. It can be labor

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Peano, Andrea. "S1.2c Diagnosis of fungal infections in animals: Combining the old and the new to maximize results." Medical Mycology 60, Supplement_1 (2022). http://dx.doi.org/10.1093/mmy/myac072.s1.2c.

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Abstract S1.2 Emerging and Expanding Endemic Mycoses, September 21, 2022, 11:00 AM - 12:30 PM There is a broad spectrum of fungal infections involving companion, zootechnical and wild animals. Some fungi are distributed worldwide and act as opportunistic pathogens. Others, such as the dimorphic fungi Blastomyces dermatitidis and Sporothrix brasiliensis, are primary pathogens with a more defined geographical distribution. Dermatophytes cause less severe diseases limited to the skin. However, they are relevant since they are widely diffused. Moreover, some dermatophytes are transmitted from anim

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sprotocols. "Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots." January 7, 2015. https://doi.org/10.5281/zenodo.13799.

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Authors: Alyona Sukhanova, Klervi Even-Desrumeaux, Patrick Chames, Daniel Baty, Mikhail Artemyev, Vladimir Oleinikov & Igor Nabiev ### Abstract Nanoparticle-based biodetection commonly employs monoclonal antibodies (mAbs) for targeting. Although several types of conjugates have been used for biomarker labeling, the large size of mAbs limits the number of ligands per nanoparticle, impedes their intratumoral distribution, and limits intracellular penetration. Furthermore, the conditions of mAb conjugation using conventional techniques provide nanoprobes with irregular orientation of mAbs on

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Tanchuco, Joven Jeremius Q. "Quo Vadis, COVID-19?" Acta Medica Philippina 54, no. 2 (2021). http://dx.doi.org/10.47895/amp.v54i2.4474.

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The World Health Organization (WHO) declared a COVID-19 pandemic last March 11, 2020.1,2 According to the WHO Director General, “In the past two weeks, the number of cases of COVID-19 outside China has increased 13-fold, and the number of affected countries has tripled. There are now more than 118,000 cases in 114 countries, and 4,291 people have lost their lives. Thousands more are fighting for their lives in hospitals.” Soon after, Metro Manila was placed on a complete lockdown which started on March 15, 2020 and continues up to the time of this writing.2 So, what exactly is this COVID

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sprotocols. "High Resolution Melting Analysis for fast and cheap polymorphism screening of marine populations." January 14, 2015. https://doi.org/10.5281/zenodo.13940.

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Authors: Anne-Leila Meistertzheim, Isabelle Calves, Sébastien Artigaud, Carolyn S. Friedman, Christine Paillard, Jean Laroche & Claude Ferec ### Abstract This protocol permits the mutation scanning of PCR products by high-resolution DNA melting analysis requiring the inclusion of a saturating intercalating dye in the PCR mix without labelled probe. During a scanning process, fluorescent melting curves of PCR amplicons are analyzed. Mutations modifying melting curve shapes, are allowed to be further characterized by sequencing because melting is not destructive. The method detects on small

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