Log in

Relevant bibliographies by topics / Molecular Probe Techniques / Journal articles

To see the other types of publications on this topic, follow the link: Molecular Probe Techniques.

Journal articles on the topic 'Molecular Probe Techniques'

Author: Grafiati

Published: 4 June 2021

Last updated: 30 July 2024

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Molecular Probe Techniques.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Reynolds, Fred, and Kimberly A. Kelly. "Techniques for Molecular Imaging Probe Design." Molecular Imaging 10, no. 6 (2011): 7290.2011.00003. http://dx.doi.org/10.2310/7290.2011.00003.

Full text

Abstract:

Molecular imaging allows clinicians to visualize disease-specific molecules, thereby providing relevant information in the diagnosis and treatment of patients. With advances in genomics and proteomics and underlying mechanisms of disease pathology, the number of targets identified has significantly outpaced the number of developed molecular imaging probes. There has been a concerted effort to bridge this gap with multidisciplinary efforts in chemistry, proteomics, physics, material science, and biology—all essential to progress in molecular imaging probe development. In this review, we discuss

APA, Harvard, Vancouver, ISO, and other styles

2

Asryadin, Asryadin, Nilasari Indah Yuniati, Nur Aini Hidayah Khasanah, et al. "Bioinformatics Techniques for Developing Molecular Detection Methods for the HIV-1 Gag Gene." European Journal of Biomedical Research 3, no. 4 (2024): 1–4. http://dx.doi.org/10.24018/ejbiomed.2024.3.4.97.

Full text

Abstract:

The HIV-1 Gag gene, which plays an essential role in HIV replication, can be detected accurately using qRT-PCR. The quality of qRTPCR analysis is determined by the primers and probes used for DNA amplification. This research aims to use bioinformatics techniques to design primer pair sequences and qRT-PCR probes for HIV detection using the HIV-1 Gag gene. HIV-1 Gag gene sequences were obtained from HIV-1 isolates and serotypes, downloaded from the National Center for Biotechnology Information (NCBI) GenPeptd nucleotide database. Sequences were then examined using the ClustalW algorithm of the

APA, Harvard, Vancouver, ISO, and other styles

3

Yanagisawa, Keisuke, Ryunosuke Yoshino, Genki Kudo, and Takatsugu Hirokawa. "Inverse Mixed-Solvent Molecular Dynamics for Visualization of the Residue Interaction Profile of Molecular Probes." International Journal of Molecular Sciences 23, no. 9 (2022): 4749. http://dx.doi.org/10.3390/ijms23094749.

Full text

Abstract:

To ensure efficiency in discovery and development, the application of computational technology is essential. Although virtual screening techniques are widely applied in the early stages of drug discovery research, the computational methods used in lead optimization to improve activity and reduce the toxicity of compounds are still evolving. In this study, we propose a method to construct the residue interaction profile of the chemical structure used in the lead optimization by performing “inverse” mixed-solvent molecular dynamics (MSMD) simulation. Contrary to constructing a protein-based, ato

APA, Harvard, Vancouver, ISO, and other styles

4

Yanagisawa, Keisuke, Ryunosuke Yoshino, Genki Kudo, and Takatsugu Hirokawa. "Inverse Mixed-Solvent Molecular Dynamics for Visualization of the Residue Interaction Profile of Molecular Probes." International Journal of Molecular Sciences 23, no. 9 (2022): 4749. http://dx.doi.org/10.3390/ijms23094749.

Full text

Abstract:

To ensure efficiency in discovery and development, the application of computational technology is essential. Although virtual screening techniques are widely applied in the early stages of drug discovery research, the computational methods used in lead optimization to improve activity and reduce the toxicity of compounds are still evolving. In this study, we propose a method to construct the residue interaction profile of the chemical structure used in the lead optimization by performing “inverse” mixed-solvent molecular dynamics (MSMD) simulation. Contrary to constructing a protein-based, ato

APA, Harvard, Vancouver, ISO, and other styles

5

Woods, Gail L. "Molecular Techniques in Mycobacterial Detection." Archives of Pathology & Laboratory Medicine 125, no. 1 (2001): 122–26. http://dx.doi.org/10.5858/2001-125-0122-mtimd.

Full text

Abstract:

Abstract Objective.—To assess the clinical utility of the commercial nucleic acid amplification (NAA) tests (ie, Amplified Mycobacterium Tuberculosis Direct Test, Gen-Probe, Inc and AMPLICOR Mycobacterium tuberculosis Test, Roche Molecular Systems, Inc) for direct detection of Mycobacterium tuberculosis complex. Data Sources.—Review of the English-language literature. Conclusions.—The performance of both NAA tests is excellent (sensitivity, ≥95%; specificity, 100%) when testing respiratory specimens that are smear-positive for acid-fast bacilli (AFB). Only the Gen-Probe assay is approved for t

APA, Harvard, Vancouver, ISO, and other styles

6

Beaudette, Patrick, Oliver Popp, and Gunnar Dittmar. "Proteomic techniques to probe the ubiquitin landscape." PROTEOMICS 16, no. 2 (2015): 273–87. http://dx.doi.org/10.1002/pmic.201500290.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Karjalainen, Reijo, Leo Rouhiainen, and Hans Söderlund. "Diagnosis of plant viruses by nucleic acid hybridization." Agricultural and Food Science 59, no. 3 (1987): 179–91. http://dx.doi.org/10.23986/afsci.72262.

Full text

Abstract:

Nucleic acid hybridization is a powerful technique for the diagnosis of many plant viruses not easily detected by serological techniques. It is particularly effective in the detection of viruses occurring in low amount in plant tissue, viruses that are poor immunogens or contain satellites. Molecular probes with desired specificities can be prepared by recombinant DNA techniques for large scale use. cDNA probes of potato virus X(PVX) RNA were made by molecular cloning, and the clones were 32P labelled by nick translation. Hybridization of cDNA to PVX RNA revealed 1 ng of purified virus in 2 µl

APA, Harvard, Vancouver, ISO, and other styles

8

Leake, Mark C., and Steven D. Quinn. "A guide to small fluorescent probes for single-molecule biophysics." Chemical Physics Reviews 4, no. 1 (2023): 011302. http://dx.doi.org/10.1063/5.0131663.

Full text

Abstract:

The explosive growth of single-molecule techniques is transforming our understanding of biology, helping to develop new physics inspired by emergent biological processes, and leading to emerging areas of nanotechnology. Key biological and chemical processes can now be probed with new levels of detail, one molecule at a time, from the nanoscopic dynamics of nature's molecular machines to an ever-expanding range of exciting applications across multiple length and time scales. Their common feature is an ability to render the underlying distribution of molecular properties that ensemble averaging

APA, Harvard, Vancouver, ISO, and other styles

9

Lai, Rebecca Y., Bryce Walker, Kent Stormberg, Anita J. Zaitouna, and Weiwei Yang. "Electrochemical techniques for characterization of stem-loop probe and linear probe-based DNA sensors." Methods 64, no. 3 (2013): 267–75. http://dx.doi.org/10.1016/j.ymeth.2013.07.041.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Cragg, G., H. S. Kwon, C. Y. Dong, and P. T. C. So. "Breaking the Resolution Limit of Far Field Optical Microscopy: Imaging Molecular and Cellular Interatction on the Nanometer Scale." Microscopy and Microanalysis 5, S2 (1999): 504–5. http://dx.doi.org/10.1017/s1431927600015841.

Full text

Abstract:

Historically, high-resolution optical microscopy has played a vital role in biomedical research. The resolution of conventional optical microscopy is limited by the diffraction effect to about 200 nm and 500-900 nm in the radial and axial directions, respectively. This modest resolution is a major limitation in using optical microscopy for demanding applications. New techniques such as near field optical microscopy, scanning tunneling microscopy, and atomic force microscopy have been developed to circumvent this problem and have achieved atomic resolution in solid state specimens. Unfortunatel

APA, Harvard, Vancouver, ISO, and other styles

11

Jahan, Nusrat, Hanwei Wang, Shensheng Zhao, et al. "Optical force microscopy: combining light with atomic force microscopy for nanomaterial identification." Nanophotonics 8, no. 10 (2019): 1659–71. http://dx.doi.org/10.1515/nanoph-2019-0181.

Full text

Abstract:

AbstractScanning probe techniques have evolved significantly in recent years to detect surface morphology of materials down to subnanometer resolution, but without revealing spectroscopic information. In this review, we discuss recent advances in scanning probe techniques that capitalize on light-induced forces for studying nanomaterials down to molecular specificities with nanometer spatial resolution.

APA, Harvard, Vancouver, ISO, and other styles

12

XIE, XIAN NING, HONG JING CHUNG, and ANDREW THYE SHEN WEE. "SCANNING PROBE MICROSCOPY BASED NANOSCALE PATTERNING AND FABRICATION." COSMOS 03, no. 01 (2007): 1–21. http://dx.doi.org/10.1142/s0219607707000207.

Full text

Abstract:

Nanotechnology is vital to the fabrication of integrated circuits, memory devices, display units, biochips and biosensors. Scanning probe microscope (SPM) has emerged to be a unique tool for materials structuring and patterning with atomic and molecular resolution. SPM includes scanning tunneling microscopy (STM) and atomic force microscopy (AFM). In this chapter, we selectively discuss the atomic and molecular manipulation capabilities of STM nanolithography. As for AFM nanolithography, we focus on those nanopatterning techniques involving water and/or air when operated in ambient. The typica

APA, Harvard, Vancouver, ISO, and other styles

13

Xiao, Ying, Adya P. Singh, and Robin N. Wakeling. "Detecting Bacteria in Wood using a Fluorescent Lipid Probe." Microscopy Today 6, no. 7 (1998): 16–17. http://dx.doi.org/10.1017/s1551929500068632.

Full text

Abstract:

Wood cells have strong autofluorescence in a wide wavelength band due to lignin in the cell walls. The detection of microorganisms in wood is very difficult when using fluorescent microscopy because of interferences. We have recently developed fluorescent staining techniques to differentiate fungal hyphae from wood cell walls (Singh, et al., 1997; Xiao, et al., 1997). This study was aimed at developing fluorescent techniques to visualize bacteria in wood using confocal laser scanning microscopy (CLSM). Nitrobenzoxadiazole glycerophosphoethanolamine (NBD-PE, Molecular Probes), a widely used mem

APA, Harvard, Vancouver, ISO, and other styles

14

Richter, Elvira, Stefan Niemann, Sabine Rüsch-Gerdes, and Sven Hoffner. "Identification of Mycobacterium kansasiiby Using a DNA Probe (AccuProbe) and Molecular Techniques." Journal of Clinical Microbiology 37, no. 4 (1999): 964–70. http://dx.doi.org/10.1128/jcm.37.4.964-970.1999.

Full text

Abstract:

The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of this new formulation was 97.4% and the specificity was 100%. Still, three M. kansasii strains were missed by this probe. To evaluate the variability within the species, genetic analyses of the hsp65 gene, the spacer sequence between the 16S and 23S rRNA genes, and the 16S rRNA ge

APA, Harvard, Vancouver, ISO, and other styles

15

Scherer, N. F., J. W. Perry, F. E. Doany, and Ahmed H. Zewail. "Ultrafast molecular relaxation of isolated stilbene: measurements by picosecond pump-probe techniques." Journal of Physical Chemistry 89, no. 6 (1985): 894–96. http://dx.doi.org/10.1021/j100252a004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Kellett, Andrew, Zara Molphy, Creina Slator, Vickie McKee, and Nicholas P. Farrell. "Molecular methods for assessment of non-covalent metallodrug–DNA interactions." Chemical Society Reviews 48, no. 4 (2019): 971–88. http://dx.doi.org/10.1039/c8cs00157j.

Full text

Abstract:

Herein we provide an accessible account of molecular methods to probe inorganic–nucleic acid interactions. Techniques are described using copper(ii) and platinum(ii) complexes prepared in our laboratories.

APA, Harvard, Vancouver, ISO, and other styles

17

Jahan Zeb Baber, Imran Ali, Zahida Qasim, et al. "Epidemiology of Molecular Probes in Xpert MTB/RIF Assay in AJK, Pakistan." Annals of PIMS-Shaheed Zulfiqar Ali Bhutto Medical University 19, no. 2 (2023): 72–78. http://dx.doi.org/10.48036/apims.v19i2.519.

Full text

Abstract:

Objective: This study aimed to detect rifampicin-resistant tuberculosis cases and assess the frequency of missing probes in different study populations in Azad Jammu and Kashmir (AJK), Pakistan. Methodology: The study was conducted at the State TB Reference Laboratory, District Headquarters Teaching Hospital, Mirpur, AJK. A total of 2,790 specimens collected between March 2016 to August 2019 were analyzed. Pulmonary TB (PTB) accounted for 94% of the cases, while 6% were classified as extra-pulmonary cases. All respiratory and non-respiratory samples underwent fluorescence smear microscopy (AFB

APA, Harvard, Vancouver, ISO, and other styles

18

Kim, Byung I. "Nanotribology and nanoindentation using advanced scanning probe techniques." Scanning 32, no. 5 (2010): v—vi. http://dx.doi.org/10.1002/sca.20213.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Fu, Xiaoni, Yinmin Shi, Jiying Ma, et al. "Advances of multiplex ligation-dependent probe amplification technology in molecular diagnostics." BioTechniques 73, no. 4 (2022): 205–13. http://dx.doi.org/10.2144/btn-2022-0017.

Full text

Abstract:

Multiplex ligation-dependent probe amplification (MLPA) is a multiplex copy number analysis tool which is routinely used to detect large mutations in genetic diseases. With continuous modifications, MLPA has been extended for the detection of DNA methylation variation, single nucleotide polymorphisms, chromosome abnormalities and other forms of genomic variation. The combination with other techniques has even enlarged the application of MLPA in molecular diagnostics of various human diseases. In this review, the principle of MLPA-based techniques as well as their main and latest applications i

APA, Harvard, Vancouver, ISO, and other styles

20

Bogliş, Alina, Florin Tripon, and Claudia Bănescu. "The utility of molecular genetic techniques in craniosynostosis cases associated with intellectual disability." Revista Romana de Medicina de Laborator 26, no. 4 (2018): 471–77. http://dx.doi.org/10.2478/rrlm-2018-0033.

Full text

Abstract:

Abstract Molecular genetic testing in craniosynostosis leads to the detection of the mutations in the genes encoding fibroblast growth factor receptors (FGFR), providing information about the etiology of the genetic disorder. Muenke syndrome is produced by p.Pro250Arg mutation in FGFR3 gene with evidence of variable expressivity, representing 8% of the syndromic craniosynostoses. Here, we present the identification of a p.Pro250Arg pathogenic mutation (c.749C>G) in the FGFR3 gene using Multiplex Ligation-dependent Probes Amplification (MLPA) analysis in conjunction with Sanger sequencing in

APA, Harvard, Vancouver, ISO, and other styles

21

Yan, Yuling, M. Emma Marriott, Chutima Petchprayoon, and Gerard Marriott. "Optical switch probes and optical lock-in detection (OLID) imaging microscopy: high-contrast fluorescence imaging within living systems." Biochemical Journal 433, no. 3 (2011): 411–22. http://dx.doi.org/10.1042/bj20100992.

Full text

Abstract:

Few to single molecule imaging of fluorescent probe molecules can provide information on the distribution, dynamics, interactions and activity of specific fluorescently tagged proteins during cellular processes. Unfortunately, these imaging studies are made challenging in living cells because of fluorescence signals from endogenous cofactors. Moreover, related background signals within multi-cell systems and intact tissue are even higher and reduce signal contrast even for ensemble populations of probe molecules. High-contrast optical imaging within high-background environments will therefore

APA, Harvard, Vancouver, ISO, and other styles

22

Faltin, Bernd, Roland Zengerle, and Felix von Stetten. "Current Methods for Fluorescence-Based Universal Sequence-Dependent Detection of Nucleic Acids in Homogenous Assays and Clinical Applications." Clinical Chemistry 59, no. 11 (2013): 1567–82. http://dx.doi.org/10.1373/clinchem.2013.205211.

Full text

Abstract:

BACKGROUND Specific and sensitive nucleic acid (NA) testing in research and clinical diagnostics is usually performed by use of labeled oligonucleotide probes. However, the use of target-specific fluorogenic probes increases the cost of analysis. Therefore, universal sequence-dependent (USD) NA detection methods have been developed to facilitate cost-effective target detection using standardized reagents. CONTENT We provide a comprehensive review of the current methods for fluorescence-based USD NA detection. Initially, we focus on the emergence of these methods as a means to overcome the shor

APA, Harvard, Vancouver, ISO, and other styles

23

Schiffman, Joshua D., Hanlee Ji, Katrina M. Welch, et al. "Novel Allele Quantification Method To Classify Childhood Leukemia." Blood 108, no. 11 (2006): 2273. http://dx.doi.org/10.1182/blood.v108.11.2273.2273.

Full text

Abstract:

Abstract Background: Leukemia is the most common pediatric malignancy, accounting for nearly 40% of all new childhood cancer. The cure rates for pediatric ALL have increased to more than 80% and the cure rates for pediatric AML now approach 50%. Much of this progress can be attributed to cytogenetic and molecular risk stratification with subsequent randomized control trials. Genomic instability events may also serve as relevant prognostic biomarkers. A novel high-throughput genomic technology called Molecular Inversion Probes (MIPs) quantifies genomic instability, gene copy number and allelic

APA, Harvard, Vancouver, ISO, and other styles

24

Tedder, Sarah A., Markus C. Weikl, Thomas Seeger, and Alfred Leipertz. "Determination of probe volume dimensions in coherent measurement techniques." Applied Optics 47, no. 35 (2008): 6601. http://dx.doi.org/10.1364/ao.47.006601.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Meaney, Paul, Robin Augustine, Adrian Welteke, Bernd Pfrommer, Adam M. Pearson, and Helena Brisby. "Transmission-Based Vertebrae Strength Probe Development: Far Field Probe Property Extraction and Integrated Machine Vision Distance Validation Experiments." Sensors 23, no. 10 (2023): 4819. http://dx.doi.org/10.3390/s23104819.

Full text

Abstract:

We are developing a transmission-based probe for point-of-care assessment of vertebrae strength needed for fabricating the instrumentation used in supporting the spinal column during spinal fusion surgery. The device is based on a transmission probe whereby thin coaxial probes are inserted into the small canals through the pedicles and into the vertebrae, and a broad band signal is transmitted from one probe to the other across the bone tissue. Simultaneously, a machine vision scheme has been developed to measure the separation distance between the probe tips while they are inserted into the v

APA, Harvard, Vancouver, ISO, and other styles

26

Sreehari Sastry, S., V. Venkata Rao, P. Narayana Murty, G. Satyanandam, and T. F. Sundar Raj. "Molecular Ordering in Nematic MBCA and EPAPU." Zeitschrift für Naturforschung A 45, no. 1 (1990): 29–32. http://dx.doi.org/10.1515/zna-1990-0106.

Full text

Abstract:

AbstractBy EPR, two nematic liquid crystals (MBCA and EPAPU) were investigated using a steroidal nitroxide spin probe. In both liquid crystals the isotropic-nematic phase transition is of first order. The observed variation of the order parameter with temperature is compared with predictions from the Maier-Saupe and Humphries-James-Luckhurst models and with results obtained by several other experimental techniques.

APA, Harvard, Vancouver, ISO, and other styles

27

Sheet, Partha Sarathi, Suji Park, Pavel Sengupta, and Dipankar Koley. "Multifunctional dendritic molecular probe for selective detection of Cu²⁺ ions using potentiometric and fluorometric techniques." Analyst 146, no. 23 (2021): 7109–17. http://dx.doi.org/10.1039/d1an01417j.

Full text

Abstract:

We have designed and synthesized a multifunctional dendritic molecular probe that selectively detects Cu2+ ions via potentiometric and fluorometric techniques with low detection limits (3.5 μM in potentiometry, 15 nM in fluorometry).

APA, Harvard, Vancouver, ISO, and other styles

28

Aoiz, F. J., M. Brouard, S. D. S. Gordon, B. Nichols, S. Stolte, and V. Walpole. "A new perspective: imaging the stereochemistry of molecular collisions." Physical Chemistry Chemical Physics 17, no. 45 (2015): 30210–28. http://dx.doi.org/10.1039/c5cp03273c.

Full text

Abstract:

The concept of the steric effect plays a central role in chemistry. This Perspective describes how the polarization of reactant molecules in space can be used to probe directly the steric effect, and highlights some of the new measurements that are made possible by coupling reactant orientation and alignment with ion imaging techniques.

APA, Harvard, Vancouver, ISO, and other styles

29

Valiati Barreto, Cynthia Aparecida, Marco Antônio Peixoto, Késsia Leite de Souza, Natália Martins Travenzoli, Renato Neves Feio, and Jorge Abdala Dergam. "Further insights into chromosomal evolution of the genus Enyalius with karyotype description of Enyalius boulengeri Etheridge, 1969 (Squamata, Leiosauridae)." Caryologia 74, no. 3 (2021): 169–75. http://dx.doi.org/10.36253/caryologia-1120.

Full text

Abstract:

The genus Enyalius is composed of 11 described species inhabiting forest areas in Amazônia, Cerrado and Atlantic forest biomes. Currently, eight species with high levels of chromosome variation have been karyotyped. The study aims to characterize the karyotype of Enyalius boulengeri, with classical and molecular techniques, and improve knowledge about the karyotype evolution of the lizard genus Enyalius. The species has 2n = 36 chromosomes (8m + 4sm + 24mc), FN = 24; NORs and 18S rDNA were subtelomeric and located on chromosome pair 2. Repetitive DNA probes (CAT)10 accumulated on centromeric a

APA, Harvard, Vancouver, ISO, and other styles

30

Magonov, S. "Industrial Applications of Scanning Probe Microscopy." Microscopy and Microanalysis 4, S2 (1998): 522–23. http://dx.doi.org/10.1017/s143192760002273x.

Full text

Abstract:

The evolution of scanning tunneling microscopy (STM) into atomic force microscopy (AFM) have led to a family of scanning probe techniques which are widely applied in fundamental research and in industry. Visualization of the atomic- and molecular-scale structures and the possibility of modifying these structures using a sharp probe were demonstrated with the techniques on many materials. These unique capabilities initiated the further development of AFM and related methods generalized as scanning probe microscopy (SPM). The first STM experiments were performed in the clean conditions of ultra-

APA, Harvard, Vancouver, ISO, and other styles

31

Matos, Tiago, Vânia Pinto, Paulo Sousa, et al. "Design and In Situ Validation of Low-Cost and Easy to Apply Anti-Biofouling Techniques for Oceanographic Continuous Monitoring with Optical Instruments." Sensors 23, no. 2 (2023): 605. http://dx.doi.org/10.3390/s23020605.

Full text

Abstract:

Biofouling is the major factor that limits long-term monitoring studies with automated optical instruments. Protection of the sensing areas, surfaces, and structural housing of the sensors must be considered to deliver reliable data without the need for cleaning or maintenance. In this work, we present the design and field validation of different techniques for biofouling protection based on different housing materials, biocides, and transparent coatings. Six optical turbidity probes were built using polylactic acid (PLA), acrylonitrile butadiene styrene (ABS), PLA with copper filament, ABS co

APA, Harvard, Vancouver, ISO, and other styles

32

Nezammahalleh, Hassan, Faezeh Ghanati, Shima Rezaei, et al. "Biochemical Interactions through Microscopic Techniques: Structural and Molecular Characterization." Polymers 14, no. 14 (2022): 2853. http://dx.doi.org/10.3390/polym14142853.

Full text

Abstract:

Many researchers and scientists have contributed significantly to provide structural and molecular characterizations of biochemical interactions using microscopic techniques in the recent decade, as these biochemical interactions play a crucial role in the production of diverse biomaterials and the organization of biological systems. The properties, activities, and functionalities of the biomaterials and biological systems need to be identified and modified for different purposes in both the material and life sciences. The present study aimed to review the advantages and disadvantages of three

APA, Harvard, Vancouver, ISO, and other styles

33

Beggs, Marjorie L., Rossina Stevanova, and Kathleen D. Eisenach. "Species Identification of Mycobacterium avium Complex Isolates by a Variety of Molecular Techniques." Journal of Clinical Microbiology 38, no. 2 (2000): 508–12. http://dx.doi.org/10.1128/jcm.38.2.508-512.2000.

Full text

Abstract:

Organisms in the Mycobacterium avium complex (MAC;M. avium, M. intracellulare, and “nonspecific or X” MAC) are emerging pathogens among individual organisms of which significant genetic variability is displayed. The objective of the present study was to evaluate various molecular methods for the rapid and definitive identification of MAC species. Isolates were obtained from both human immunodeficiency virus (HIV)-positive patients and HIV-negative patients with and without known predisposing conditions. The isolates were initially hybridized with nucleic acid probes complementary to the rRNA o

APA, Harvard, Vancouver, ISO, and other styles

34

Moldovan, Valeriu, and Elena Moldovan. "Multiplex ligation-dependent probe amplification – a short overview." Revista Romana de Medicina de Laborator 28, no. 2 (2020): 123–31. http://dx.doi.org/10.2478/rrlm-2020-0016.

Full text

Abstract:

AbstractMultiplex Ligation-dependent Probe Amplification is a technique proposed for the detection of deletions or duplications that may lead to copy number variations in genomic DNA, mainly due to its higher resolution, and shorter overall diagnosis time, when compared with techniques traditionally used, namely karyotyping, fluorescence in situ hybridization, and array comparative genomic hybridization. Multiplex Ligation-dependent Probe Amplification is a fast (about 2 days), useful and cost-effective technique, being suitable for the diagnosis of hereditary conditions caused by complete or

APA, Harvard, Vancouver, ISO, and other styles

35

Kondzior, Mateusz, and Iwona Grabowska. "Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors." Sensors 20, no. 7 (2020): 2014. http://dx.doi.org/10.3390/s20072014.

Full text

Abstract:

Suitable immobilization of a biorecognition element, such as an antigen or antibody, on a transducer surface is essential for development of sensitive and analytically reliable immunosensors. In this review, we report on (1) methods of antibody prefunctionalization using electroactive probes, (2) methods for immobilization of such conjugates on the surfaces of electrodes in electrochemical immunosensor construction and (3) the use of antibody-electroactive probe conjugates as bioreceptors and sensor signal generators. We focus on different strategies of antibody functionalization using the red

APA, Harvard, Vancouver, ISO, and other styles

36

Watkins, Tylan S., Dipobrato Sarbapalli, Michael J. Counihan, et al. "A combined SECM and electrochemical AFM approach to probe interfacial processes affecting molecular reactivity at redox flow battery electrodes." Journal of Materials Chemistry A 8, no. 31 (2020): 15734–45. http://dx.doi.org/10.1039/d0ta00836b.

Full text

Abstract:

Understanding interfacial reaction mechanisms of redoxmers at redox flow battery model carbon electrodes using insightful electrochemical scanning probe techniques enables new strategies for high-performance energy storage.

APA, Harvard, Vancouver, ISO, and other styles

37

Hartmann, J. M., J. Vander Auwera, C. Boulet, et al. "Infrared absorption by molecular gases to probe porous materials and comparisons with other techniques." Microporous and Mesoporous Materials 237 (January 2017): 31–37. http://dx.doi.org/10.1016/j.micromeso.2016.09.014.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Hall, David B., R. D. Miller, and John M. Torkelson. "Molecular probe techniques for studying diffusion and relaxation in thin and ultrathin polymer films." Journal of Polymer Science Part B: Polymer Physics 35, no. 17 (1997): 2795–802. http://dx.doi.org/10.1002/(sici)1099-0488(199712)35:17<2795::aid-polb4>3.0.co;2-q.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Kahlke, Tim, Paavo Jumppanen, Ralf Westram, Guy C. G. Abell, and Levente Bodrossy. "ProbeSpec: batch specificity testing and visualization of oligonucleotide probe sets implemented in ARB." F1000Research 7 (December 6, 2018): 1901. http://dx.doi.org/10.12688/f1000research.16905.1.

Full text

Abstract:

High-throughput molecular methods such as quantitative polymerase chain reaction (qPCR) and environmental microarrays are cost-effective methods for semi-quantitative assessment of bacterial community structure and the identification of specific target organisms. Both techniques rely on short nucleotide sequences, so-called oligonucleotide probes, which require high specificity to the organisms in question to avoid cross-hybridization with non-target taxa. However, designing oligonucleotide probes for novel taxa or marker genes that show sufficient phylogenetic sensitivity and specificity is o

APA, Harvard, Vancouver, ISO, and other styles

40

Milsom, Adam, Adam M. Squires, Jack Macklin, Paul Wady, and Christian Pfrang. "Acoustic levitation combined with laboratory-based small-angle X-ray scattering (SAXS) to probe changes in crystallinity and molecular organisation." RSC Advances 14, no. 25 (2024): 17519–25. http://dx.doi.org/10.1039/d4ra01418a.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Rosati, Barbara, Frederic Grau, Anneke Kuehler, Samantha Rodriguez, and David McKinnon. "Comparison of different probe-level analysis techniques for oligonucleotide microarrays." BioTechniques 36, no. 2 (2004): 316–22. http://dx.doi.org/10.2144/04362mt03.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Rivera, Yago, Maxime Bidon, José-Luis Muñoz-Cobo, Cesar Berna, and Alberto Escrivá. "A Comparative Analysis of Conductance Probes and High-Speed Camera Measurements for Interfacial Behavior in Annular Air–Water Flow." Sensors 23, no. 20 (2023): 8617. http://dx.doi.org/10.3390/s23208617.

Full text

Abstract:

Different techniques are used to analyze annular flow, but the more interesting ones are those techniques that do not perturb the flow and provide enough resolution to clearly distinguish the interfacial phenomena that take place at the interface, especially the disturbance waves (DW) and the ripple waves (DW). The understanding of these events is important because it influences the heat and mass transfer taking place through the thin film formed near the walls in this flow regime. The laser-induced fluorescence (LIF) and the three-electrode conductance probe are two commonly used techniques t

APA, Harvard, Vancouver, ISO, and other styles

43

MacNeil, L., T. Kauri, and W. Robertson. "Molecular techniques and their potential application in monitoring the microbiological quality of indoor air." Canadian Journal of Microbiology 41, no. 8 (1995): 657–65. http://dx.doi.org/10.1139/m95-091.

Full text

Abstract:

Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This review discusses the shortcomings of current methods of identification and detection and focuses on the potential for molecular techniques in this emerging field. Probe techniques, restriction endonuclease analysis, karyotyping, and DNA and polymerase chain reaction fingerprinting

APA, Harvard, Vancouver, ISO, and other styles

44

Ouellette, Marc, John J. Rossi, Renée Bazin та Paul H. Roy. "Oligonucleotide probes for the detection of TEM-1 and TEM-2 β-lactamase genes and their transposons". Canadian Journal of Microbiology 33, № 3 (1987): 205–11. http://dx.doi.org/10.1139/m87-035.

Full text

Abstract:

We describe the use of molecular probes to detect the TEM-type β-lactamase genes. As a general probe, we prepared a 656 base pair restriction fragment, entirely within the TEM structural gene. This probe was specific for the TEM family, hybridizing only with TEM-1 and TEM-2. The TEM-1 and TEM-2 β-lactamases differ by only one amino acid. We synthesized two oligonucleotides whose central bases correspond to this difference. The use of these oligonucleotides enables us to discriminate between TEM-1 and TEM-2 genes. Using oligonucleotides homologous to parts of Tn3, we also monitored the presence

APA, Harvard, Vancouver, ISO, and other styles

45

Frank Haverkort, C., and Christopher Gilpin. "A unique strain of Mycobacterium tuberculosis and a cautionary tale for users of molecular techniques." Microbiology Australia 25, no. 4 (2004): 32. http://dx.doi.org/10.1071/ma04432.

Full text

Abstract:

Molecular techniques are now widely applied in Australia and elsewhere for the direct detection of Mycobacterium tuberculosis DNA in clinical specimens and culturally enhanced material. All nucleic acid testing methods have the potential to give false negative results due to mutations that may arise at primer or probe binding sites. We describe one such strain of M. tuberculosis that was encountered in 1995 and that has not been encountered in Australia since.

APA, Harvard, Vancouver, ISO, and other styles

46

Merrill, F. E. "Imaging with penetrating radiation for the study of small dynamic physical processes." Laser and Particle Beams 33, no. 3 (2015): 425–31. http://dx.doi.org/10.1017/s0263034615000282.

Full text

Abstract:

AbstractSince Roentgen's discovery of X rays in the late 1800s the use of penetrating radiation to form images has become a part of our everyday life as well as providing a useful tool for the scientific study of processes that have been previously impossible to measure. This can include the study of processes that are too deeply embedded in opaque materials for direct observation, or that occur on a length or time scale smaller than otherwise can be easily measured. As technologies to generate penetrating radiation and quickly collect images have matured, new techniques have emerged to measur

APA, Harvard, Vancouver, ISO, and other styles

47

Uchizono, N. M., R. E. Wirz, A. L. Collins, C. Marrese-Reading, S. M. Arestie, and J. K. Ziemer. "A diagnostic for quantifying secondary species emission from electrospray devices." Review of Scientific Instruments 94, no. 2 (2023): 025008. http://dx.doi.org/10.1063/5.0117666.

Full text

Abstract:

Measuring the polydisperse beam of charged species emitted by an electrospray device requires accurate measurements of current. Secondary species emission (SSE) caused by high-velocity nanodroplet or molecular ion impacts on surfaces contributes to substantial uncertainty in current measurements. SSE consists of both positive and negative species; hence, mitigating measurement uncertainty requires different considerations other than plasma diagnostic techniques. The probe and analysis methods described herein distinguish between current contributions from positive SSE, negative SSE, and primar

APA, Harvard, Vancouver, ISO, and other styles

48

Hassan, Muntaha M., Mushtak T. S. Al-Ouqaili, Nidhal A. Mohammed, and Omar A. Mohammed. "Advanced and conventional molecular techniques in the diagnosis of Mycoplasma pneumoniae in patients with respiratory tract infection." Journal of the Faculty of Medicine Baghdad 56, no. 1 (2014): 95–100. http://dx.doi.org/10.32007/jfacmedbagdad.561438.

Full text

Abstract:

Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy.Objective: This study has been undertaken to detect M. pneumoniae in respiratory samples (throat swabs, throat wash and sputum) in patients with respiratory tract infection qualitatively by conventional polymerase chain reaction (PCR). Also, more advanced one, real time PCR was used to determine mycoplasmal target gene qualitatively and quantitatively.P

APA, Harvard, Vancouver, ISO, and other styles

49

Nikiforova, Alena, and Igor Sedov. "Molecular Design of Magnetic Resonance Imaging Agents Binding to Amyloid Deposits." International Journal of Molecular Sciences 24, no. 13 (2023): 11152. http://dx.doi.org/10.3390/ijms241311152.

Full text

Abstract:

The ability to detect and monitor amyloid deposition in the brain using non-invasive imaging techniques provides valuable insights into the early diagnosis and progression of Alzheimer’s disease and helps to evaluate the efficacy of potential treatments. Magnetic resonance imaging (MRI) is a widely available technique offering high-spatial-resolution imaging. It can be used to visualize amyloid deposits with the help of amyloid-binding diagnostic agents injected into the body. In recent years, a number of amyloid-targeted MRI probes have been developed, but none of them has entered clinical pr

APA, Harvard, Vancouver, ISO, and other styles

50

Bartoš, Josef, Bożena Zgardzińska, Helena Švajdlenková, et al. "A Combined Atomic and Molecular Probe Characterization of Aromatic Hydrocarbons via PALS and ESR: Methylbenzene." Materials 15, no. 2 (2022): 462. http://dx.doi.org/10.3390/ma15020462.

Full text

Abstract:

A combined study of one of the simplest aromatic hydrocarbons, i.e., methylbenzene (toluene) (TOL), via the annihilation of an ortho-positronium (o-Ps) probe via positron annihilation lifetime spectroscopy (PALS) and the rotation dynamics of nitroxide spin probe 2,2,6,6-tetramethyl-piperidinyl-1-oxy (TEMPO) using electron spin resonance (ESR) over a wide temperature range, 10–300 K, is reported. The o-Ps lifetime, τ3, and the relative o-Ps intensity, I3, as a function of temperature exhibit changes defining several characteristic PALS temperatures in the slowly and rapidly cooled samples. Simi

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!