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Dissertations / Theses on the topic 'Molecular probes'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Nathubhai, Amit. "Molecular probes for mammalian chitinases." Thesis, University of Bath, 2010. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.518107.

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Chitin is a glycopolymer consisting of +-(1, 4)-linked N-acetyl-D-glucosamine residues that occurs widely in nature and is a constituent of many organisms that are pathogenic to humans, including insects, fungi, and parasitic nematodes. As these organisms depend on the ability to break down chitin at key points of their life-cycle, inhibitors of the enzymes termed chitinases that catalyse the hydrolysis of chitin, are of considerable interest as potential drugs and insecticides. Although chitin is absent from mammalian physiology, two human chitinases along with several chitin-binding proteins

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2

Sapkal, S. "Spectroscopic investigation of molecular probes." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2021. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/6010.

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3

Wang, Lei. "Molecular Probes for Pancreatic Cancer Imaging." PDXScholar, 2016. http://pdxscholar.library.pdx.edu/open_access_etds/3108.

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Pancreatic ductal adenocarcinoma (PDAC) has the poorest five-year survival rate of any cancer. Currently, there are no effective diagnostics or chemotherapeutics. Surgical resection is the only curative therapy. However, most patients experience recurrence due largely to challenges in assessing tumor margin status in the operating room. Molecular probes that selectively highlight pancreatic cancer tissue, having the potential to improve PDAC margin assessment intraoperatively, are urgently needed. In this work, a series of red and near-infrared fluorescent probes is reported. Two were found to

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4

Willcocks, T. C. "Mapping of human genes with molecular probes." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370272.

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5

James, David Thomas. "Developing structural probes for designed molecular architectures." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/10732.

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This thesis is concerned with the development of techniques to probe and control molecular morphology in organic semiconductors and investigate structure-property relationships. An angle-dependent polarised Raman technique is developed to probe molecular order and orientation in 6,13-bis(triisopropylsilylethynyl) pentacene (TIPS-pentacene) thin lms, and applied to understand the role of blending with polystyrene on morphology and charge transport properties in inkjet-printed organic field-effect transistors (OFETs). Compared to pristine devices, blending improves film uniformity in terms of de

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6

Caries, Christopher Cain. "Organometallics : a platform for molecular imaging probes /." May be available electronically:, 2009. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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7

Chang, Yuan-Pin. "Novel probes of angular momentum polarization." Thesis, University of Oxford, 2010. http://ora.ox.ac.uk/objects/uuid:d3880edf-436a-415e-8a74-6b1c0fd26e65.

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New dynamical applications of quantum beat spectroscopy (QBS) to molecular dynamics are employed to probe the angular momentum polarization effects in photodissociation and molecular collisions. The magnitude and the dynamical behaviour of angular momentum alignment and orientation, two types of polarization, can be measured via QBS technique on a shot-by-shot basis. The first part of this thesis describes the experimental studies of collisional angular momentum depolarization for the electronically excited state radicals in the presence of the collider partners. Depolarization accompanies bot

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8

Kujala, Naresh Gandhi Yu Ping. "Frequency domain fluorescent molecular tomography and molecular probes for small animal imaging." Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/7021.

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Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 26, 2010). The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Dissertation advisor: Dr. Ping Yu. Vita. Includes bibliographical references.

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9

Mastrodonato, Cristiano Matteo. "Elaboration of fluorescent molecular probes and molecular-based nanoparticles for bioimaging purposes." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0652/document.

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Les techniques de fluorescence sont des outils de choix pour l’étude et la compréhension fine des processus biologiques. Ceci requiert toutefois l’utilisation de sondes fluorescentes parfaitement adaptées au but visé et répondant aux différentes exigences requises pour l’application visée. Dans ce cadre, nous nous sommes plus particulièrement intéressés à l’élaboration de sondes biphotoniques de pH adaptées à une mesure très sensible de faibles variations de pH autour du pH neutre. Les variations et gradients de pH sont en effet impliqués dans un certain nombre de processus biologiques importa

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10

Lenaerts, Jeremy. "Molecular beacons as hybridisation probes in microbial ecology." Thesis, University of Exeter, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.493638.

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Microorganisms are of fundamental importance to global biogeochemical cycling, yet aspects of their ecology are poorly understood. Within the present methods used to study microbial ecology are limitations with regards to sensitivity, applicability and automation. This study aimed to investigate the potential application of novel nucleic acid probes (molecular beacons and locked nucleic acid probes) in microbial ecology.

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11

Allen, Nicholas Denby. "Transgenes as molecular probes of mouse developmental genetics." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335023.

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12

Shchepinova, Maria M. "Molecular probes for monitoring mitochondrial movement and function." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7835/.

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This thesis explores two distinct parts of mitochondrial physiology: the role of mitochondria in generation of reactive oxygen species (ROS) and mitochondrial morphology and dynamics within cells. The first area of research is covered in Chapters 1-8. Mitochondrial biofunctionality and ROS production are discussed in Chapter 1, followed by the strategy of targeting bioactive compounds to mitochondria by linking them to lipophilic triphenylphosphonium cations (TPP) (Chapter 2). ROS sensors relevant to the research are reviewed in Chapter 3. Chapter 4 presents design and synthesis of novel probe

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13

Cowell, Sheena. "Development of molecular probes responsive to matrix metalloproteinases." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/23890.

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Matrix metalloproteinases (MMPs) have been identified as biomarkers for cancer, offering prognostic potential. However, they lack non-invasive detection. This thesis highlights the work carried out on the design, synthesis and development of 19F MRI probes for the molecular imaging of MMP activity. The design of these MMP-activated imaging agents was based on the paramagnetic effects of gadolinium(III) on the NMR/MRI signal of a CF3 group in close proximity to the metal cation. 1st generation probes were prepared, incorporating MMP-2 selective peptide substrates SPAYYTA or SLAYYTA as a linker

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14

Savage, Michelle L. "Synthesis and development of light-activated molecular probes." Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:3532bd21-ad68-4e8e-9e77-9fd6b620d4f3.

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Judicious addition of photolabile caging groups (PCGs) to protect biologically im- portant molecules, has enabled the development of many powerful chemical tools for the study of biological processes. These tools have the potential to be activated in a cellular setting by irradiation with light of appropriate wavelengths, restoring functionality, with excellent spatial and temporal control. This D. Phil. dissertation highlights two biologically relevant examples where PCGs can be applied: (i) 4,5-dimethoxynitrobenzyl (DMNB) caged derivatives of GSH and ESG, were synthesised and uncaging of the

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15

Gawel, Justyna M. "Targeted molecular probes for investigating biological redox processes." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/7000/.

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This thesis explores molecular probes for understanding redox biology and oxidative stress. It is divided into eleven chapters and a reference section. The first two chapters comprise the general background of mitochondria, oxidative stress and strategies for targeting bioactive molecules to mitochondria (Chapter 1) and to the extracellular environment (Chapter 2). The work presented in Chapters 3-5 includes the synthesis of extracellulary-targeted antioxidants (ExCell antioxidants) in the University of Glasgow. The targeting strategy is novel and was confirmed by biological work performed in

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16

Martinez, Diaz Karen. "Polymer linker DNA probe design, characterization and comparison with molecular beacons." [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0010881.

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17

Peng, Hanjing. "Development of Bacterial Quorum Sensing Inhibitors and Molecular Probes." Digital Archive @ GSU, 2012. http://digitalarchive.gsu.edu/chemistry_diss/73.

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Bacterial quorum sensing is regarded as a novel target for the design of antimicrobials. Based on lead structures identified from HTS, 39 analogues have been synthesized and evaluated in Vibrio haveyi. Potent inhibitors with IC50 values at single-digit micromolar concentrations for AI-2 mediated quorum sensing have been identified. On the second project, post-synthesis modifications of DNA provide easy functionalizations for expanded applications such as aptamer selection. A CBT-modified thymidine analogue (CBT-TTP) has been synthesized and used for enzymatic incorporation into DNA. Post-synth

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18

Sim, Neil. "Molecular imaging probes for N-methyl-D-aspartate receptors." Thesis, Durham University, 2014. http://etheses.dur.ac.uk/10816/.

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The non-invasive detection of neuronal transmission is of prime importance in order to understand brain function better. This will aid cognitive neuroscience, as well as medical science, in the early detection of diseased states. Herein, approaches to molecular imaging of the NMDA receptor, a receptor subtype of the excitatory neurotransmitter glutamate, through the use of targeted contrast agents, is described. Initially, a series of NMDA receptor-targeted MRI contrast agents was developed based upon a known competitive NMDA receptor antagonist, appended to an N-linked ‘Gd-DOTA’ core that pos

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19

Crane, Peter. "Protein based molecular probes by unnatural amino acid incorporation." Thesis, University of Oxford, 2018. http://ora.ox.ac.uk/objects/uuid:772076fc-00f2-4ca7-bfa9-3da1ce7093cb.

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The "tag & modify" strategy for protein modification relies upon the genetic incorporation of an uncommon or unnatural amino acid into a protein backbone, followed by a chemo-selective modification to yield differentially modified proteins. This thesis describes the creation of a protein-based glycoconjugate tool for interrogating biological function. In Chapter 2, the unnatural amino acid, azidohomoalanine was genetically incorporated into a library of distance defined Np276 proteins via a selective pressure incorporation. Methods to prevent the common post translational modification N-termin

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20

Zhu, Wenxia. "Development of Molecular Imaging Probes for Positron Emission Tomography." Case Western Reserve University School of Graduate Studies / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=case1333944487.

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21

Torode, Thomas Adrian. "Molecular probes for the cell walls of brown algae." Thesis, University of Leeds, 2014. http://etheses.whiterose.ac.uk/12218/.

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The brown algae are an abundant source of renewable biomass that do not require arable land or fresh water for cultivation. Cell walls of the brown algae contain a range of unique polysaccharides with useful bioactivities; namely the sulphated fucans and alginate. The generation and characterisation of ten new monoclonal antibodies (MAbs) towards components of brown algal cell walls are described. Four MAbs, BAM1 to BAM4, recognise un-sulphated (BAM1) and sulphated (BAM2/3/4) structures of the sulphated fucans. Six MAbs, GIN1 to GIN6, recognise different structures of the alginate, ranging fro

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22

Vaccaro, Carlos. "Use of luminescence energy transfer probes to detect genetic variants." Thesis, University of North Texas, 2004. https://digital.library.unt.edu/ark:/67531/metadc4566/.

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The purpose of this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, the formation of an alternative complex structure such as a dimer. Of all three factors, dimer formation is the most troublesome, since it reduces the emission of the reporter molecules. A new probe design was used to reduce dimer formation. The emission signal of the improved probe was se

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23

Exton, S. P. "Synthesis and characterisation of novel luminescent probes." Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368774.

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24

Duer, Melinda J. "The parametric probes of ligand field theory." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304165.

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25

Jain, Mudita 1968. "Algorithms for physical mapping using unique probes." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/290622.

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DNA molecules are sequences of characters over a four letter alphabet. Determining the text of the DNA sequence contained in human cells is the goal of the Human Genome Project. The structure of a DNA sequence is reconstructed from a set of shorter fragments sampled from it at unknown locations, as it is usually too long to be determined directly. We consider the problem when the the fragments are very long, and each fragment has a fingerprint consisting of the presence of two or three pre-selected, smaller sequences called probes within it. These probes have a unique location along the origin

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26

Sulistyowati, Emy. "Development of molecular probes to distinguish vesicular-arbuscular mycorrhizal fungi." Title page, Summary and Contents only, 1995. http://web4.library.adelaide.edu.au/theses/09A/09as949.pdf.

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Bibliography: leaves 71-79. Almost 80 percent of plant taxa develop vesicular-arbuscular mycorrhizae (VAM) which are symbiotic associations between plant roots and soil fungi. The fungi are biotropic-obligate symbionts. Identification of VAM fungi is currently based on spore characteristics. Molecular techniques provide tools for better and more accurate identification of species, as well as for the examination of genetic variability occuring between individual spores of a single species.

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27

Stanton-Humphreys, Megan. "Design, synthesis and application of novel light-activated molecular probes." Thesis, University of Oxford, 2010. http://ora.ox.ac.uk/objects/uuid:1d0905c4-7fba-4796-92a5-848715204950.

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Caged compounds are biologically active molecules that are rendered inert by masking an important functionality with a photolabile protecting, ‘caging’, group. The caging group can be removed by irradiation with light to reveal the active compound with restored pharmacological activity with high spatial and temporal control. This technology provides an ideal tool for the study of many chemical, physiological and biological systems. This DPhil dissertation highlights several projects in which caging technology has been employed to address biological problems and questions. The first example of

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28

Guo, Qi. "Improving the discovery of molecular imaging probes through biomathematical modelling." Thesis, University of Oxford, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543482.

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29

Bacigalupo, Maria Candelaria Rogart. "Locked nucleic acid analogues for novel exciplex-based molecular probes." Thesis, University of Manchester, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538490.

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30

Fediv, V. I. "Quantum dot as the basis of multimodal molecular imaging probes." Thesis, БДМУ, 2021. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/18844.

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31

Ogawa, Michihiro. "Photovoltaic devices and molecular imaging probes based on polymeric nanomaterials." 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120889.

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32

Hedayat, Farzaneh. "Investigation of the molecular probes for PM oxidative capacity measurements." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/98552/1/Farzaneh_Hedayat_Thesis.pdf.

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This thesis improved our understanding of the performance of various assays that are employed to investigate the oxidative potential (OP) of the particulate matter (PM) generated from combustion sources. The OP is expressed through the concentration of reactive oxygen species produced by diesel and biodiesel fuels. Although the combustion of the biofuel generates a lower amount of PM, the particles have a higher OP, with the increase being proportional to the oxygen content of the biofuel. Oxygen content does not only influence the OP of the particle phase but also influences the OP of the gas

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33

Bielanska, Magdalena M. "Telomeric probes for FISH, technical aspects and clinical applications." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0026/MQ37095.pdf.

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34

Hirata, Nao. "Molecular Imaging Probes and Radiation-Activated Prodrugs Targeting Tumor Hypoxic Microenvironments: Molecular Design, Synthesis, and Evaluation." 京都大学 (Kyoto University), 2009. http://hdl.handle.net/2433/84827.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(工学)<br>甲第14870号<br>工博第3138号<br>新制||工||1470(附属図書館)<br>27292<br>UT51-2009-K666<br>京都大学大学院工学研究科物質エネルギー化学専攻<br>(主査)教授西本清一, 教授大江浩一, 教授中條善樹<br>学位規則第4条第1項該当

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35

Shi, Chao. "Molecular fiber sensor probes based on surface enhanced Raman scattering (SERS) /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2009. http://uclibs.org/PID/11984.

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36

Bai, Ge. "Cell Permeability Studies of AApeptides and Novel Molecular Probes for AD." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6173.

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Alzheimer's diseases(AD) has been discovered and under research for more than 70 years, However there is no cure for these progressive and devastating diseases. Based on the following hypothsis: Aß metabolite problem/over production result in the accumulation, and lead to aggregation is the cause of Alzheimer’s disease. AApeptide and Melatonin derivatives can bind to Aß and block the aggregation of β amyloid monomers, decrease the toxicity of Aß to neurons and slow the progressive of Alzheimer’s diseases. In addition, AApeptide which mimic transmembrane peptide Tat will have similar transmem

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37

Adams, Elizabeth L. "Developing molecular probes in arabinoxylan structural analysis using Atomic Force Microscopy." Thesis, University of East Anglia, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393318.

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38

Wainman, Yéléna Alexandra. "Developing novel chemical probes for molecular imaging of glycans in cancer." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708565.

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39

Yang, Chaoyong. "Molecular engineering of nucleic acid probes for intracellular imaging and bioanalysis." [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0015562.

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40

Moorhouse, Alan. "Molecular hydrogen line ratios as probes of shocks in dense clouds." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/28656.

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This thesis is concerned with the structure of shocks occurring in dense regions of molec?ular clouds. These shocks are associated with the outflows from young stars, Herbig-Haro objects, expanding HII regions and the interaction of supernovae remnants with molecu?lar clouds. Momentum, mass and energy are imparted to the cloud. A full understanding of the shock process is thus needed if we are to understand the structure of molecular clouds and the impact on star formation. Emission from the near-infrared transitions of molecular hydrogen is commonly excited in these shocks. A major puzzle is

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41

Chakraborty, Sourav. "Molecular Probes for Biologically Important Molecules: A Study of Thiourea, Hydroxyl radical, Peroxynitrite and Hypochlorous acid." ScholarWorks@UNO, 2010. http://scholarworks.uno.edu/td/1132.

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Numerous chemical species are important to the health of biological systems. Some species can be beneficial at low doses and harmful at high doses. Other species are highly reactive and trigger serious cell damage. Improved methods to detect the presence and activity of such species are needed. In this work, several biologically important species were studied using appropriate analytical techniques. Fluoride is an important species in human physiology. It strengthens teeth and gives protection against dental caries. However, elevated concentrations of fluoride in the body can lead to he

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42

Mackay, Martha. "The development of fluorescent probes targeting Caspase-3 for detecting apoptosis." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/17972.

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The design and development of fluorescent reporters focussed on highly sensitive, specific, and selective imaging of cancer targets is described. These novel optical molecular probes were synthesised with the aim of creating bio-imaging breakthroughs that will aid the clinical analysis of cancer. A specific target of the project was to develop fluorescent reporters for Caspases; intracellular endopeptidases that play an essential role in apoptosis. Lack of activation of the ‘Caspase Cascade’ causes uncontrolled proliferation of cells and has been deemed a ‘Hallmark of Cancer’. In particular, l

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43

Torres, Martínez Ana. "Molecular nanogels as nanocarriers. Intracellular transport of photosensitizers and nitric oxide probes." Doctoral thesis, Universitat Jaume I, 2021. http://hdl.handle.net/10803/671255.

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The preparation of molecular nanogels is reported in this thesis. These new nanoparticles, formed by low molecular weight gelators, represent an exciting alternative to their polymeric counterparts considering their intrinsic characteristics (fully reversible stimuli-regulated assembly, easy biodegradation, excellent biocompatibility and tailored properties). Novel hydrogel@liposome particles were also obtained by molecular gel formation inside liposomes, merging the benefits of liposomal vehicles with the inherent characteristics of molecular gels. The capability of molecular nanogels to inco

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44

Jannasch, Anita. "High performance photonic probes and applications of optical tweezers to molecular motors." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-103696.

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Optical tweezers are a sensitive position and force transducer widely employed in physics and biology. In a focussed laser, forces due to radiation pressure enable to trap and manipulate small dielectric particles used as probes for various experiments. For sensitive biophysical measurements, microspheres are often used as a handle for the molecule of interest. The force range of optical traps well covers the piconewton forces generated by individual biomolecules such as kinesin molecular motors. However, cellular processes are often driven by ensembles of molecular machines generating forces

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45

Wagner, Janine. "Migration of molecular probes from can coatings into foods and food simulants." Thesis, University of Leeds, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.496207.

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46

Okoh, Okoh Adeyi. "The synthesis and evaluation of non-targeted near-infrared heptamethine molecular probes." Thesis, University of Central Lancashire, 2013. http://clok.uclan.ac.uk/9232/.

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Detecting and quantifying biomolecules is an important tool in biological research. Ideal dyes have to have a number of characteristics i.e. low toxicity and limited autofluorescence. The properties of near infrared dyes make these ideal for cell and tissue imaging. The work within this thesis focuses on the development of three families of NIR dyes (linear, rigid and substituted polymethine) and these are actively compared against the clinical standard Indocyanine Green (ICG) and its structural derivative New Indocyanine Green (IR-820). The ultimate aim being to identify dyes which can be use

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47

Arnberg, Elise. "Investigation of molecular probes for pH determination with electrospray ionization mass spectrometry." Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-381962.

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48

Condie, Allison Gamble. "Development of Molecular Probes for Biomedical Imaging of Cancer and Neurological Disease." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1432726238.

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49

Kim, Youngmi. "Molecular engineering of multifunctional nucleic acid probes for bioanalytical and biomedical applications." [Gainesville, Fla.] : University of Florida, 2008. http://purl.fcla.edu/fcla/etd/UFE0023647.

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50

GAMBINO, GIUSEPPE. "High-relaxivity systems and molecular imaging probes for Magnetic Resonance Imaging applications." Doctoral thesis, Università del Piemonte Orientale, 2014. http://hdl.handle.net/11579/46171.

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