Academic literature on the topic 'Molecular taxonomy'

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Journal articles on the topic "Molecular taxonomy"

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Deák, T. "MOLECULAR TAXONOMY OF YEASTS." Acta Microbiologica et Immunologica Hungarica 46, no. 2-3 (May 1999): 181–86. http://dx.doi.org/10.1556/amicr.46.1999.2-3.3.

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Wink, M., E. Käss, and M. Kaufmann. "Molecular versus Chemical Taxonomy." Planta Medica 59, S 1 (December 1993): A594—A595. http://dx.doi.org/10.1055/s-2006-959803.

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Gómez Daglio, Liza, and Michael N. Dawson. "Integrative taxonomy: ghosts of past, present and future." Journal of the Marine Biological Association of the United Kingdom 99, no. 06 (April 26, 2019): 1237–46. http://dx.doi.org/10.1017/s0025315419000201.

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AbstractDescribing species has been a formal, intellectually rich and influential applied and basic area of study for many of the past 260 years. While formally described eukaryotic diversity still falls short of estimated eukaryotic species diversity by many hundreds of thousands of species, some recent accounts have suggested a growing number of taxonomists are within reach of describing all extant species. We present a case study that illustrates, to the contrary, a recent ‘taxonomic impediment’ in part attributable to derogation of taxonomy as a scientific discipline: contemporary practice has re-interpreted taxonomy largely as an endeavour in enumerating species. We argue that challenges lie in (1) a poor understanding of taxonomy's epistemology; (2) excessive displacement of interest toward ecological or molecular studies; (3) over-interpretation of the contributions of multiple authors describing a species; and (4) perspectives that are strongly influenced by well-known taxa. The historical and recent literature on scyphozoans reveal ghosts of taxonomy's past that persist in the present, but suggest also that a renaissance enabled by integrative taxonomy is possible in the (near) future.
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Akram, Seemab, Shamsul Khamis, Shahrizim Zulkifly, Rishzuan Talib, and Nurul Izza Ab Ghani. "Phylogeny Study of 20 Selected Species of Zingiberaceae from Ex situ Collections in Peninsular Malaysia." Pertanika Journal of Tropical Agricultural Science 46, no. 1 (February 22, 2023): 329–45. http://dx.doi.org/10.47836/pjtas.46.1.18.

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Zingiberaceae is widely distributed in Malaysia, with 750 species and 31 genera. This family comprises a different number of subfamilies and genera according to different taxonomic classification methods — classical taxonomy: one subfamily and four tribes vs molecular taxonomy: four subfamilies and six tribes. However, the taxonomic classification of Zingiberaceae is still debated, especially the classical taxonomy. It is due to some Zingiberaceae species showing cryptic morphologies that make it difficult to classify them through classical taxonomy, which refers to the unique morphological characteristics of a tribe/species. Therefore, accurate taxonomic classification is required by using a molecular approach. In this study, 20 selected species of Zingiberaceae collected from the Agricultural Conservatory Park, Institute of Bioscience (IBS), Universiti Putra Malaysia (UPM) were taxonomically classified using a molecular method with the help of three random amplified polymorphic DNA (RAPD) and three inter simple sequence repeat (ISSR) markers until the tribe level. The combined RAPD and ISSR unweighted pair group method with arithmetic mean (UPGMA) phylogenetic tree was comparable to Zingiberaceae’s current molecular and classical taxonomy. The 20 selected species were grouped into three tribes (Alpinieae, Zingiberaceae, and Globbeae). This finding has contributed additional biological information to better manage the 20 Zingiberaceae species in the Agricultural Conservatory Park, IBS, UPM. Further studies are needed to explore the genetic diversities and properties of Zingiberaceae species.
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HÖFLING, José F., Edvaldo A. R. ROSA, Mirian J. BAPTISTA, and Denise M. P. SPOLIDÓRIO. "New Strategies on Molecular Biology Applied to Microbial Systematics." Revista do Instituto de Medicina Tropical de São Paulo 39, no. 6 (November 1997): 345–52. http://dx.doi.org/10.1590/s0036-46651997000600007.

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Systematics is the study of diversity of the organisms and their relationships comprising classification, nomenclature and identification. The term classification or taxonomy means the arrangement of the organisms in groups (rate) and the nomenclature is the attribution of correct international scientific names to organisms and identification is the inclusion of unknown strains in groups derived from classification. Therefore, classification for a stable nomenclature and a perfect identification are required previously. The beginning of the new bacterial systematics era can be remembered by the introduction and application of new taxonomic concepts and techniques, from the 50’s and 60’s. Important progress were achieved using numerical taxonomy and molecular taxonomy. Molecular taxonomy, brought into effect after the emergence of the Molecular Biology resources, provided knowledge that comprises systematics of bacteria, in which occurs great evolutionary interest, or where is observed the necessity of eliminating any environmental interference. When you study the composition and disposition of nucleotides in certain portions of the genetic material, you study searching their genome, much less susceptible to environmental alterations than proteins, codified based on it. In the molecular taxonomy, you can research both DNA and RNA, and the main techniques that have been used in the systematics comprise the build of restriction maps, DNA-DNA hybridization, DNA-RNA hybridization, sequencing of DNA sequencing of sub-units 16S and 23S of rRNA, RAPD, RFLP, PFGE etc. Techniques such as base sequencing, though they are extremely sensible and greatly precise, are relatively onerous and impracticable to the great majority of the bacterial taxonomy laboratories. Several specialized techniques have been applied to taxonomic studies of microorganisms. In the last years, these have included preliminary electrophoretic analysis of soluble proteins and isoenzymes, and subsequently determination of deoxyribonucleic acid base composition and assessment of base sequence homology by means of DNA-RNA hybrid experiments beside others. These various techniques, as expected, have generally indicated a lack of taxonomic information in microbial systematics. There are numberless techniques and methodologies that make bacteria identification and classification study possible, part of them described here, allowing establish different degrees of subspecific and interspecific similarity through phenetic-genetic polymorphism analysis. However, was pointed out the necessity of using more than one technique for better establish similarity degrees within microorganisms. Obtaining data resulting from application of a sole technique isolatedly may not provide significant information from Bacterial Systematics viewpoint
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Guého, Eveline, M. C. Leclerc, G. S. Hoog, and B. Dupont. "Molecular taxonomy and epidemiology ofBlastomycesandHistoplasmaspecies." Mycoses 40, no. 3-4 (September 1997): 69–81. http://dx.doi.org/10.1111/j.1439-0507.1997.tb00191.x.

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Méndez-Álvarez, Sebastián, Sergio Ramírez-Moreno, and Núria Gaju. "Molecular Taxonomy of the GenusChlorobium." Critical Reviews in Microbiology 27, no. 1 (January 2001): 9–24. http://dx.doi.org/10.1080/20014091096675.

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Harley, E. H. "DNA APPROACHES TO MOLECULAR TAXONOMY." Transactions of the Royal Society of South Africa 46, no. 4 (January 1988): 283–90. http://dx.doi.org/10.1080/00359198809520131.

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Lahbib-Mansais, Yvette, Mireille Mata, and Paul Ritzenthaler. "Molecular taxonomy of Lactobacillus phages." Biochimie 70, no. 3 (March 1988): 429–35. http://dx.doi.org/10.1016/0300-9084(88)90217-9.

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Kurtzman, Cletus P. "Molecular taxonomy of the yeasts." Yeast 10, no. 13 (December 1994): 1727–40. http://dx.doi.org/10.1002/yea.320101306.

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Dissertations / Theses on the topic "Molecular taxonomy"

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Scholfield, Claire Ingrid. "Molecular Taxonomy of the Rhodophyta." Thesis, Cardiff University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310158.

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Muthumeenakshi, Sreenivasaprasad. "Molecular taxonomy of the genus Trichoderma." Thesis, Queen's University Belfast, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264087.

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Dhanasekaran, Vijaykrishna. "Freshwater fungi biodiversity, origins and molecular taxonomy /." Thesis, Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B32017583.

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Hodgetts, Jennfier. "The taxonomy of phytoplasmas : a molecular approach." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508216.

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Silva, Bruna Demari e. "Estudos taxonômicos de espécies do gênero Culex (Diptera: Culicidae) da região neotropical, utilizando a subunidade I do gene mitocondrial citocromo oxidase." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/6/6132/tde-28012010-105230/.

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Diversas espécies de mosquitos do gênero Culex Linnaeus são vetores de nematóides que causam filariose linfática (Wuchereria bancrofti) e de vários arbovírus, incluindo o Vírus do Nilo Ocidental (VNO) que causa encefelites em animais e humanos. Embora seja o maior gênero da família Culicidae, com 763 espécies conhecidas, pouco se sabe sobre a taxonomia e as relações filogenéticas do grupo. Considerando-se a grande diversidade de espécies do gênero Culex, as dificuldades para a identificação morfológica, devidas principalmente ao fato das fêmeas serem morfologicamente muito similares, o presente trabalho teve como objetivos: (1) Solucionar problemas relacionados à nomenclatura; (2) Estimar as relações filogenéticas entre espécies de diferentes subgêneros; (3) Examinar o monofiletismo de subgêneros da Região Neotropical; (4) Estimar as relações evolutivas entre subgêneros neotropicais; (5) Estimar a posição filogenética do gênero Lutzia em relação à Culex; (6) Discutir sobre a utilização do gene citocromo c oxidase da subunidade I (COI) para o gênero Culex. Foram analisadas sequências correspondentes a um fragmento de 478 pares de bases do gene COI de 36 indivíduos pertencentes a 16 espécies do gênero Culex. Foram avaliadas espécies de quatro subgêneros, Culex, Phenacomyia, Melanoconion e Microculex e uma espécie do gênero Lutzia. As sequências do gene COI foram comparadas através das análises de Máxima Parcimônia, Máxima Verossimilhança e Bayesiana. Os resultados das análises das sequências de COI, utilizando ao modelo de Kimura 2-parâmetros, de Culex dolosus, Culex mollis e Culex imitator, demonstram a presença de divergências intraespecíficas altas (3,1%, 2,3% e 3,5%, respectivamente). Os valores do modelo Kimura 2-parâmetros indicam que esses taxa podem representar complexos de espécies. As topologias de MV, MP e Bayesiana mostraram que tanto o gênero Culex como o subgênero Culex são parafiléticos, pois o primeiro não inclui o gênero Lutzia e o segundo exclui o Phenacomyia. Os resultados indicam que Lutzia é subgênero de Culex e Phenacomyia um grupo monofilético do subgênero Culex. O marcador molecular COI foi de fácil utilização e análise, provando ser ferramenta útil para estudos filogenéticos e para a taxonomia molecular de Culex
Species of the genus Culex Linnaeus mosquitoes have been pointed out as the main vectors of lymphatic filariases. Furthermore, they are important vectors of encephalitis across the world, including the West Nile Virus. Although being the major genus in Culicidae family, with 763 valid species, Culex, in a taxonomic and phylogenetic sense, is one of the least known. Considering the great diversity of species of mosquitoes in this genus, and the fact that females of several species are vary similar morphologically, the present study aimed to: (1) Solve problems related to nomenclature (2) estimate the phylogenetic relationships of species used in the work; (3) examine the monophyly of subgenera of the Neotropical Region; (4) estimate the evolutionary relationships between neotropical subgenera; (5) estimate the phylogenetic position of the genus Lutzia in the Culex, (6) discuss the use of the gene cytochrome c oxidase subunit I (COI) to the genus Culex. We analyzed sequences corresponding to a fragment of 478 base pairs of the COI gene of 36 individuals belonging to 16 species of the genus Culex. Species were evaluated in four subgenera, Culex, Phenacomyia, Melanoconion and Microculex and one species of the genus Lutzia. The COI gene sequences were compared using analysis of Maximum Parsimony, Maximum Likelihood and Bayesian. The results of the analysis of the COI sequences, used the model of Kimura 2-parameters of Culex dolosus, Culex mollis and Culex imitator, demonstrate the presence of high intraspecific divergence (3.1%, 2.3% and 3.5% respectively). These values indicate that these taxa may represent complexes of species. The topologies of ML, MP and Bayesian showed that both genus Culex as subgenus Culex are paraphyletic because the first does not include the genus Lutzia and the second excludes the Phenacomyia subgenus. The results indicate that Lutzia is a subgenus of Culex and Phenacomyia is a monophyletic group of subgenus Culex. The molecular marker COI was easy to use and analyzing, proving to be useful tool for phylogenetic studies and the molecular taxonomy of Culex
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Cao, Wenguang. "Wheat taxonomy and cultivar identification using molecular markers." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24007.pdf.

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Krüger, Manuela. "Molecular phylogeny, taxonomy and evolution of arbuscular mycorrhizal fungi." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-140769.

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Jeewon, Rajesh. "Pestalotiopsis taxonomy : molecular phylogenetics, species nomenclature and teleomorph relationships /." Thesis, Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B24367394.

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Cai, Junpeng. "Molecular phylogenetic analysis on some ascomycetous spoilage yeasts." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297332.

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Lee, Chung-Kun. "Phylogeny and Taxonomy of Commelinaceae (Commelinales)." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263508.

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Books on the topic "Molecular taxonomy"

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Besse, Pascale, ed. Molecular Plant Taxonomy. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-767-9.

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Besse, Pascale, ed. Molecular Plant Taxonomy. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-0997-2.

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Hewitt, Godfrey M., Andrew W. B. Johnston, and J. Peter W. Young, eds. Molecular Techniques in Taxonomy. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7.

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NATO Advanced Study Institute on Molecular Techniques in Taxonomy (1990 Norwich, England). Molecular techniques in taxonomy. Berlin: Springer-Verlag, 1991.

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Sneh, Baruch, Suha Jabaji-Hare, Stephen Neate, and Gerda Dijst, eds. Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-017-2901-7.

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Dietzgen, Ralf Georg, and Ivan V. Kuzmin. Rhabdoviruses: Molecular taxonomy, evolution, genomics, ecology, host-vector interactions, cytopathology, and control. Norfolk, UK: Caister Academic Press, 2012.

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J, Towner K., Bergogne-Bérézin E, Fewson C. A, Federation of European Microbiological Societies., and International Workshop on Acinetobacter (2nd : 1990 : Paris, France), eds. The Biology of acinetobacter: Taxonomy, clinical importance, molecular, biology, physiology, industrial relevance. New York: Plenum Press, 1991.

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Hood, Derek W. The application of molecular biology techniques to the taxonomy of the pink pigmented facultative methylotrophs. [s.l.]: typescript, 1985.

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Ōsawa, Syōzō. Molecular phylogeny and evolution of carabid ground beetles. New York: Springer, 2003.

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Antunes Carvalho, Fernanda. Molecular Phylogeny, Biogeography and an e-Monograph of the Papaya Family (Caricaceae) as an Example of Taxonomy in the Electronic Age. Wiesbaden: Springer Fachmedien Wiesbaden, 2015. http://dx.doi.org/10.1007/978-3-658-10267-8.

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Book chapters on the topic "Molecular taxonomy"

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Ryan, Una, and Lihua Xiao. "Taxonomy and Molecular Taxonomy." In Cryptosporidium: parasite and disease, 3–41. Vienna: Springer Vienna, 2013. http://dx.doi.org/10.1007/978-3-7091-1562-6_1.

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Pagano, Marcela C., and Luiz H. Rosa. "Fungal molecular taxonomy." In Fungal Biomolecules, 311–21. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118958308.ch20.

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Murthy, K. Srinivasa, S. K. Jalali, and R. Stouthamer. "Molecular Taxonomy of Trichogrammatids." In Biological Control of Insect Pests Using Egg Parasitoids, 39–65. New Delhi: Springer India, 2013. http://dx.doi.org/10.1007/978-81-322-1181-5_3.

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Willis, Dawn B. "Taxonomy of Iridoviruses." In Molecular Biology of Iridoviruses, 1–12. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-1615-2_1.

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David, Jean R. "Past and Future of Taxonomy." In Molecular Techniques in Taxonomy, 1–3. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_1.

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Parkin, David T., and J. H. Wetton. "DNA Fingerprinting." In Molecular Techniques in Taxonomy, 145–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_10.

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Brookfield, J. F. Y. "The Statistical Interpretation of Hypervariable DNAs." In Molecular Techniques in Taxonomy, 159–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_11.

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Sbordoni, V., G. Allegrucci, and D. Cesaroni. "A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets." In Molecular Techniques in Taxonomy, 171–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_12.

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Lachaise, D., and D. Joly. "Sperm and Evolution in Drosophila: morphological and molecular aspects." In Molecular Techniques in Taxonomy, 201–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_13.

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Carson, H. L. "Episodic Evolutionary Change in Local Populations." In Molecular Techniques in Taxonomy, 217–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-83962-7_14.

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Conference papers on the topic "Molecular taxonomy"

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Bi, Wenya Linda, Samantha Hoffman, Eleanor Woodward, Joseph Driver, Sherwin Tavakol, Ayal A. Aizer, Malak Abedalthagafi, Ossama Al-Mefty, Ian F. Dunn, and Sandro Santagata. "Molecular Taxonomy of Meningioma." In 30th Annual Meeting North American Skull Base Society. Georg Thieme Verlag KG, 2020. http://dx.doi.org/10.1055/s-0040-1702452.

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Park, Jisoo, Benjamin J. Hescott, and Donna K. Slonim. "Building a Molecular Taxonomy of Disease." In BCB '17: 8th ACM International Conference on Bioinformatics, Computational Biology, and Health Informatics. New York, NY, USA: ACM, 2017. http://dx.doi.org/10.1145/3107411.3108236.

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McGuire, Brett, Michael McCarthy, John Stanton, and Marie-Aline Martin-Drumel. "MICROWAVE SPECTRAL TAXONOMY AND ASTRONOMICAL SEARCHES FOR VIBRATIONALLY-EXCITED C2S AND C3S." In 71st International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2016. http://dx.doi.org/10.15278/isms.2016.fc06.

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Portas, Jesús, Rafael Bañón, José Luis del Río, David Barros-García, and Alejandro de Carlos. "Molecular Taxonomy of Deep-Sea Swallowers of the Family Chiasmodontidae." In SIBIC 2022. Basel Switzerland: MDPI, 2022. http://dx.doi.org/10.3390/blsf2022013013.

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McCarthy, Michael, and Marie-Aline Martin-Drumel. "TO KINK OR NOT: THE SEARCH FOR LONG CHAIN CUMULENONES USING MICROWAVE SPECTRAL TAXONOMY." In 71st International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2016. http://dx.doi.org/10.15278/isms.2016.rj01.

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Montero-Astúa, Mauricio. "Molecular taxonomy: A not so straightforward path to insect species identification." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112913.

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PETROV, N. B., and I. P. VLADYCHENSKAYA. "MOLECULAR COI TAXONOMY OF FRESHWATER MUSSELS OF GENUS UNIO FROM VOLGA BASIN." In 5TH MOSCOW INTERNATIONAL CONFERENCE "MOLECULAR PHYLOGENETICSAND BIODIVERSITY BIOBANKING". TORUS PRESS, 2018. http://dx.doi.org/10.30826/molphy2018-65.

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Korneenko, E. V., А. E. Samoilov, I. V. Artyushin, M. V. Safonova, V. G. Dedkov, K. F. Khafizov, A. A. Deviatkin, V. V. Kaptelova, E. V. Pimkina, and A. S. Speranskaya. "DETECTION OF ALFACORONAVIRUSES, BETACORONAVIRUSES AND ASTROVIRUSES IN BAT FECAL SAMPLES FROM MOSCOW REGION." In Molecular Diagnostics and Biosafety. Federal Budget Institute of Science 'Central Research Institute for Epidemiology', 2020. http://dx.doi.org/10.36233/978-5-9900432-9-9-42.

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In our study we analyzed viral RNA in bat fecal samples from Moscow region (Zvenigorod district) collected in 2015. To detect various virus families and genera in bat fecal samples we used PCR amplification of viral genome fragments, followed by high-throughput sequencing. Blastn search of unassembled reads revealed the presence of viruses from families Astroviridae, Coronaviridae and Herpesviridae. Assembly using SPAdes 3.14 yields contigs of length 460–530 b.p. which correspond to genome fragments of Coronaviridae and Astroviridae. The taxonomy of coronaviruses has been determined to the genus level. We also showed that one bat can be a reservoir of several virus genuses. Thus, the bats in the Moscow region were confirmed as reservoir hosts for potentially zoonotic viruses.
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Crabtree, Kyle, and Michael McCarthy. "SPECTRAL TAXONOMY: A SEMI-AUTOMATED COMBINATION OF CHIRPED-PULSE AND CAVITY FOURIER TRANSFORM MICROWAVE SPECTROSCOPY." In 69th International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2014. http://dx.doi.org/10.15278/isms.2014.re03.

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Erst, A. S., E. Yu Mitrenina, W. Wang, M. V. Skaptsov, and V. A. Kostikova. "How many species of Eranthis (Ranunculaceae) are there in Siberia and Far East part of Russia? An integrative taxonomic approach." In Problems of studying the vegetation cover of Siberia. TSU Press, 2020. http://dx.doi.org/10.17223/978-5-94621-927-3-2020-51.

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For Siberian and Far East Eranthis systematics and taxonomy problems solving the integrative approach which includes morphometrical, cytological, molecular-philogenetical and biochemical methods has been applied. Complex obtained data has showed the genus population heterogeneity within the territory under study. Based on integrative taxonomy the new species – Eranthis tanhoensis was described. Our further research will allow study hybrids and evolution of Siberian and Far East taxa.
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Reports on the topic "Molecular taxonomy"

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Eshed, Yuval, and Sarah Hake. Exploring General and Specific Regulators of Phase Transitions for Crop Improvement. United States Department of Agriculture, November 2012. http://dx.doi.org/10.32747/2012.7699851.bard.

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The transition of plants from a juvenile to adult growth phase entails a wide range of changes in growth habit, physiological competence and composition. Strikingly, most of these changes are coordinated by the expression of a single regulator, micro RNA 156 (miR156) that coordinately regulates a family of SBP genes containing a miR156 recognition site in the coding region or in their 3’ UTR. In the framework of this research, we have taken a broad taxonomic approach to examine the role of miR156 and other genetic regulators in phase change transition and its implication to plant development and crop improvement. We set to: Determine the common and unique factors that are altered upon juvenile to adult phase transition. Determine the functions of select miR156 target genes in tomato and maize, and identify those targets that mediate phase transition. Characterize the role of miR172 and its targets in tomato phase change. Determine the relationships between the various molecular circuits directing phase change. Determine the effects of regulated manipulation of phase change genes on plant architecture and if applicable, productivity. In the course of the study, a new technology for gene expression was introduced – next generation sequencing (NGS). Hence some of the original experiments that were planned with other platforms of RNA profiling, primarily Affymetrix arrays, were substituted with the new technology. Yet, not all were fully completed. Moreover, once the initial stage was completed, each group chose to focus its efforts on specific components of the phase change program. The Israeli group focused on the roles of the DELAYED SYMPODIAL TERMINATION and FALSIFLORA factors in tomato age dependent programs whereas the US group characterized in detail the role of miR156 (also termed Cg) in other grasses and in maize, its interplay with the many genes encoding miR172.
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