Dissertations / Theses on the topic 'Monolithic structure'
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Sebag, J., W. Gressler, M. Liang, D. Neill, C. Araujo-Hauck, J. Andrew, G. Angeli, et al. "LSST primary/tertiary monolithic mirror." SPIE-INT SOC OPTICAL ENGINEERING, 2016. http://hdl.handle.net/10150/622513.
Full textLiu, Xinyang. "A Monolithic Lagrangian Meshfree Method for Fluid-Structure Interaction." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1459348741.
Full textChiang, Chen-Yu. "Transport in biological systems. Monolithic method for fluid-structure interaction." Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUS477.
Full textThe present work aims at developing a numerical solver for fluid-structure interaction (FSI) problems, especially those encountered in biology such as blood circulation in valved veins. Blood flow is investigated using anatomically and physically relevant models. The first aspect of FSI problems is related to management of algorithm stability. An Eulerian monolithic formulation based on the characteristic method unconditionally achieves stability and introduce a first order in time approximation with two distinct hyperelastic material models. The second aspect deals with between-solid domain contact such as that between valve leaflets during closure and in the closed state over a finite surface, which avoid vcusp tilting and back flow. A contact algorithm is proposed and validated using benchmarks. Computational study of blood flow in valved veins is investigated, once the solver was verified and validated. The 2D computational domain comprises a single basic unit or the ladder-like model of a deep and superficial veins communicating by a set of perforating veins. A 3D mesh of the basic unit was also built. Three-dimensional computation relies on high performance computing. Blood flow dynamics is strongly coupled to vessel wall mechanics. Deformable vascular walls of large veins and arteries are composed of three main layers (intima, media, and adventitia) that consist of composite material with a composition specific to each layer. In the present work, the wall rheology is assumed to be a Mooney-Rivlin material
Ishizuka, Norio. "Studies on structure control and chromatographic properties of monolithic silica column." 京都大学 (Kyoto University), 2002. http://hdl.handle.net/2433/149779.
Full textSelmadji, Anfel. "From monolithic architectural style to microservice one : structure-based and task-based approaches." Thesis, Montpellier, 2019. http://www.theses.fr/2019MONTS026/document.
Full textSoftware technologies are constantly evolving to facilitate the development, deployment, and maintenance of applications in different areas. In parallel, these applications evolve continuously to guarantee an adequate quality of service, and they become more and more complex. Such evolution often involves increased development and maintenance costs, that can become even higher when these applications are deployed in recent execution infrastructures such as the cloud. Nowadays, reducing these costs and improving the quality of applications are main objectives of software engineering. Recently, microservices have emerged as an example of a technology or architectural style that helps to achieve these objectives.While microservices can be used to develop new applications, there are monolithic ones (i.e., monoliths) built as a single unit and their owners (e.g., companies, etc.) want to maintain and deploy them in the cloud. In this case, it is common to consider rewriting these applications from scratch or migrating them towards recent architectural styles. Rewriting an application or migrating it manually can quickly become a long, error-prone, and expensive task. An automatic migration appears as an evident solution.The ultimate aim of our dissertation is contributing to automate the migration of monolithic Object-Oriented (OO) applications to microservices. This migration consists of two steps: microservice identification and microservice packaging. We focus on microservice identification based on source code analysis. Specifically, we propose two approaches.The first one identifies microservices from the source code of a monolithic OO application relying on code structure, data accesses, and software architect recommendations. The originality of our approach can be viewed from three aspects. Firstly, microservices are identified based on the evaluation of a well-defined function measuring their quality. This function relies on metrics reflecting the "semantics" of the concept "microservice". Secondly, software architect recommendations are exploited only when they are available. Finally, two algorithmic models have been used to partition the classes of an OO application into microservices: clustering and genetic algorithms.The second approach extracts from an OO source code a workflow that can be used as an input of some existing microservice identification approaches. A workflow describes the sequencing of tasks constituting an application according to two formalisms: control flow and /or data flow. Extracting a workflow from source code requires the ability to map OO conceptsinto workflow ones.To validate both approaches, we implemented two prototypes and conducted experiments on several case studies. The identified microservices have been evaluated qualitatively and quantitatively. The extracted workflows have been manually evaluated relying on test suites. The obtained results show respectively the relevance of the identified microservices and the correctness of the extracted workflows
Bna', Simone <1985>. "Multilevel Domain Decomposition Algorithms for Monolithic Fluid-Structure Interaction Problems with Application to Haemodynamics." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6293/.
Full textGrew, Benjamin A. "Studies on monolithic tandem structure for low cost and high efficiency dye-sensitized solar cells." Thesis, Heriot-Watt University, 2016. http://hdl.handle.net/10399/3191.
Full textElsafti, Hisham [Verfasser], and H. [Akademischer Betreuer] Oumeraci. "Modelling and Analysis of Wave-Structure-Foundation Interaction for Monolithic Breakwaters / Hisham Elsafti ; Betreuer: H. Oumeraci." Braunschweig : Technische Universität Braunschweig, 2015. http://d-nb.info/117581959X/34.
Full textEverett, Judith Helen. "Structure of monolithic human insulin at 1.8A resolution and development of computer software for application in biophysics." Thesis, Liverpool John Moores University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290739.
Full textEder, Felix. "Comparing Monolithic and Event-Driven Architecture when Designing Large-scale Systems." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-300393.
Full textStrukturen som system och program designas efter är väldigt viktigt. När en arbetar med mindre grupper av system så kommer den valda arkitekturen inte att påverka prestandan mycket. Men när dessa system växter i storlek och komplexitet så kommer valet av arkitektur vara väldigt viktigt. Problem som kan uppstå när mjukvarukomplexiteten ökar är väntandet på dataaccesser, långa sekventiella exekveringar och potentiell förlust av data. Det finns ingen optimal mjukvaruarkitektur, det finns oräkneligt många sätt att designa program. Det är intressant att kolla på vilka arkitekturer som preseterar bäst sätt till exekveringstid när en hanterar ett flertal större system och stora mängder data. I den här avhandlingen kommer ett fall, kallat "Ingångsavdraget", att implementeras i en monolitisk och en event-driven arkitekturell stil och sedan köras igenom tre olika scenarion. Den monolitiska arkitekturen var vald på grund av dess enkelhet och populäritet vid utveckling av enklar program och system. Den event-drivna arkitekturen valdes på grund av vissa teoretiska fördelar, så som att kunna undvika sekventiell kommunikation mellan systemen och därmed reducera tiden som systemen väntar på svar från varandra. Den huvudsakliga forskningsfrågan som ska besvaras är vad de största fördelarna och nackdelarna är när man bygger större system med en event-driven arkitekturell stil. Andra forskningsfrågor inkludera hur arkitekturen påverkar effektiviteten hos en organisation och samarbetet mellan olika team, samt hur datasäkerheten hanteras. De två implementationerna sattes igång tre olika scenarion inom fallet, där exekveringstid, antal HTTP-anrop skickade, databasaccesser och event skickad mättes. Resultaten visar att den event-drivna arkitekturen presterade 9.4% långsamare i det första scenariot och 0.5% långsamare i det andra scenariot. I det tredje scenariot presterade den event-drivna lösningen 49.0% snabbare än den monolitiska lösningen och avslutade därmed scenariot under hälften av tiden. Den monolitiska implementationen presterade generellt väl under de simplare scenarion 1 och 2, där systemen hade färre integrationer till varandra. I dessa fallen så är den den föredragna lösningen eftersom det är lättare att designa och implementera. Den event-drivna lösningen presterade mycket bättre i det mer komplexa scenario 3, där många system och integrationer var inblandade, eftersom den kunde ta bort vissa kopplingar mellan system. Slutligen så diskuteras även hållbarhet och etik i studien, samt begränsningarna av forskningen och potentiellt framtida arbete.
Puttmann, John Paul. "Spatially Targeted Activation of a SMP." University of Dayton / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1525166147319011.
Full textSimonot, Thimothé. "Conception et hybridation de l'environnement électronique des composants de puissance à structure verticale." Thesis, Grenoble, 2011. http://www.theses.fr/2011GRENT127/document.
Full textThe work presented in this PhD manuscript deals with heterogeneous integration of the control functions for driving a vertical power MOSFET. This work consisted in the design of the driving functions for a vertical power MOSFET in a planar CMOS technology, then in the design of the power transistor itself, including specific functions for the self powering of its driving circuitry. The second aspect of this work was the technological study and realization of thick metallization at the surface of silicon dies for the chip on chip assembly of the control and the power parts. This manuscript is composed of three equal chapters: the theoretical study and concepts presentation, the design and experimental validation of the driver part, and the design of the power part and the technological realizations. The field of application of this work covers a large spectrum as it concerns every hybrid converter
Hetmer, Jakub. "Nosná konstrukce víceúčelové budovy." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2015. http://www.nusl.cz/ntk/nusl-227219.
Full textTunc, Yeliz. "Synthesis Of Acrylic Based High Internal Phase Emulsion Polymers And Their Application In Chromatography." Phd thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/2/12611133/index.pdf.
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m. The PolyHIPEs produced from the monomers of SA and IDA demonstrated elastomeric property and had high ability of recovery when the applied stress is removed. IBMA based polyHIPEs were ductile and demonstrated higher Young&rsquo
s modulus and compression strength than that of conventional styrene based polyHIPEs. Therefore, by varying the composition, it became possible to alter the mechanical properties of polyHIPEs from ductile to elastomeric, without changing the interconnected cellular structures. One of the prepared IDA based polyHIPE was evaluated as stationary phase for capillary electrochromatography for the first time in literature. The column was very efficient in the separation of alkylbenzenes namely thiourea, benzene, toluene, ethylbenzene, propylbenzene and butylbenzene with high column efficiency (up to 200.000 plates/m).
Chateau, Denis. "Etude de l'influence de la structure et de la composition de matériaux hybrides monolithiques sur les propriétés optiques (luminescence et absorption non-linéaire)." Phd thesis, Ecole normale supérieure de lyon - ENS LYON, 2013. http://tel.archives-ouvertes.fr/tel-00863807.
Full textGuepratte, Kevin. "Onduleur triphasé à structure innovante pour application aéronautique." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00647128.
Full textMayr, Matthias [Verfasser], Michael W. [Akademischer Betreuer] [Gutachter] Gee, Wolfgang A. [Gutachter] Wall, and Simone [Gutachter] Deparis. "A Monolithic Solver for Fluid-Structure Interaction with Adaptive Time Stepping and a Hybrid Preconditioner / Matthias Mayr ; Gutachter: Wolfgang A. Wall, Simone Deparis, Michael W. Gee ; Betreuer: Michael W. Gee." München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/112321090X/34.
Full textCrawford, D. L. "An inversion channel material system - toward an integrated technology : Characterisation of both optoelectronic and electronic devices fabricated from an identical inversion channel material structure and with applications in monolithic OEIC technologies." Thesis, University of Bradford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233672.
Full textEl, Feghali Stéphanie. "Nouvelle formulation monolithique en élément finis stabilisés pour l'interaction fluide-structure." Phd thesis, Ecole Nationale Supérieure des Mines de Paris, 2012. http://pastel.archives-ouvertes.fr/pastel-00743488.
Full textBroha, Vincent. "Encapsulation couche mince des dispositifs photovoltaïquesorganiques." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAI027.
Full textOxygen and water present in the atmosphere are important actors of the degradation of materialscontained in optoelectronic devices. In order to increase the stability and the lifetime ofOPV, the devices are encapsulated with gas-barrier materials by lamination encapsulation orthin film encapsulation. These latter, espacially used in OLED technology, provides high performancegas barriers by depositing dense inorganic layers directly onto the devices. However,they are subject to the defects of the surfaces on which they are deposited.The purpose of this study is to develop a planarinzing layer in order to homogenize the surfaceof organic photovoltaic devices (OPV) and to reduce the roughness with the aim to obtain animproved gas barrier protection, conferred by the subsequent deposition of dense inorganic layersby various ways (liquid and gaseous routes).In a first step, the planarization layers were developed from six p(VDF-HFP) co-polymers. Thesehave been characterized to improve our knowledge on those materials.Through a solubility study, inks at different concentrations in ethyl acetate were made. Thelatter were studied by rheological measurements and surface tension to understand better theirspread, and the surface conditions obtained on PET substrates and OPV devices. Those researchswere completed with a topography control and consequently the planarization of OPVdevices by confocal microscopy.Finally, the study of the barrier performance of hybrid encapsulation structures (organic-inorganic)revealed a good compatibility when the rugosity of the planarization layer is very low. Theseresults are confirmed by permeation measurements and accelerated aging tests of OPV devicesencapsulated in climatic chambers that illustrate the interest of the planarized ink developed.This work has been performed in the LMPO Laboratory at CEA/LITEN in collaboration withthe chemical company Arkema in order to be able to provide performant encapsulation technologies
Lagovský, Ivan. "Rekontrukce a dostavba hotelu Maximus v Brně – příprava realizace a řízení stavby." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2012. http://www.nusl.cz/ntk/nusl-225412.
Full textTomoryová, Bianka. "Facelift EDU." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2020. http://www.nusl.cz/ntk/nusl-414291.
Full textŠvehla, Ľuboš. "Vysokoškolský internát." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-226334.
Full textPappová, Martina. "Architektonická studie cyklistického stadionu /dráhy/ Favorit Brno /na volné ploše v Brně Komárově/." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2018. http://www.nusl.cz/ntk/nusl-391844.
Full textSy, Soyibou. "Algorithmes semi-implicites pour des problèmes d’interaction fluide structure : approches procédures partagées et monolithiques." Thesis, Mulhouse, 2009. http://www.theses.fr/2009MULH3092/document.
Full textOur aim was to develop some partitioned procedures and monolithic semi-implicit algorithms for solving the interaction between a fluid governed by Navier Stokes equations and a structure. In the first chapter, we propose a partitioned procedures semi-implicit algorithm for solving fluid-structure interaction problems, with a structure governed either by linear elasticity equations or by the non-linear Saint-Venant Kirchhoff model. In the second chapter, we present a partitioned procedures semi-implicit algorithm for solving fluid-structure interaction problem with a linear model for the structure and we prove an unconditional stability result of the algorithm. In the above algorithms, an optimization problem must be solved in order to get the continuity of the velocity as well as the continuity of the stress at the interface. During the iterations of BFGS for solving the optimization problem, the fluid mesh does not move and the fluid matrix is only factorized once, which reduces the computational effort. In the fast chapter, we present a monolithic semi-implicit algorithm for solving fluid-structure interaction problem with linear model for the structure. The algorithm uses one global mesh for the fluid-structure domain. The continuity of velocity at the interface is automatically satisfied and the continuity of stress does not appear explicitly in the global weak form due to the action and reaction principle. At each time step, we have to solve a monolithic system of unknowns velocity and pressure defined on the global fluid-structure domain. When the monolithic approach is used the CPU time is reduced compared to a particular partitioned procedures strategy
Munné, Vicente. "Monolithic structures formed from alumina hollow microspheres." Thesis, Georgia Institute of Technology, 1988. http://hdl.handle.net/1853/8636.
Full textMacourková, Klára. "Nosná ŽB konstrukce objektu hotelu." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2014. http://www.nusl.cz/ntk/nusl-226723.
Full textAdámek, Lukáš. "Administrativní budova." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2017. http://www.nusl.cz/ntk/nusl-265220.
Full textVaňková, Ivana. "Polyfunkční dům." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2017. http://www.nusl.cz/ntk/nusl-265651.
Full textKolb, William Blake. "The coating of monolithic structures analysis of flow phenomena /." Access abstract and link to full text, 1993. http://0-wwwlib.umi.com.library.utulsa.edu/dissertations/fullcit/9318176.
Full textMeliš, Lenka. "Dům nábytku v Trenčíně." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2018. http://www.nusl.cz/ntk/nusl-372236.
Full textMcCallef, Karl. "The dynamic response of blast-loaded monolithic and composite plated structures." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/40127.
Full textDarebníček, Pavel. "Statické řešení mostu na dálnici Jánovce-Jablonov." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-226421.
Full textBaricci, Riccardo Andrea. "Structural Analysis and Form-Finding of Mycelium-Based Monolithic Domes." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amslaurea.unibo.it/11296/.
Full textRoberge, Jean-François. "Fatigue evaluation of thick monolithic aluminum structures repaired using composite bonded doublers." [S.l. : s.n.], 2001.
Find full textRoberge, Jean-François. "Fatigue evaluation of thick monolithic aluminum structures repaired using composite bonded doublers." Mémoire, Université de Sherbrooke, 2001. http://savoirs.usherbrooke.ca/handle/11143/1295.
Full textRugeland, Patrik. "Applications of monolithic fiber interferometers and actively controlled fibers." Doctoral thesis, KTH, Laserfysik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-118750.
Full textSyftet med denna avhandling var att utveckla tillämpningar av monolitiska fiber komponenter samt aktivt kontrollerbara fiber. En speciell tvillingkärnefiber, även kallad ’Geminifiber’ användes för att konstruera fiber interferometrar med identisk armlängd som ej påverkas av termiska och mekaniska variationer. En bredbanding utbytarmultiplexor konstruerades genom att skriva in fiber Bragg gitter inuti grenarna på en Gemini Mach-Zehnder interferometer. Geminifibrer med interna metallelektroder användes för att konstruera en bredbandig nanosekundsnabb interferometrisk fiberomkopplare. Därtill användes en tvillingkärnefiber som en hög-temperatursensor. Även om komponenten direkt kan användas upp till 300 °C, måste den värmebehandlas för att kunna användas upp till 700 °C. Arbetet har innefattat utveckling, konstruktion och utvärdering av komponenterna parallellt med numeriska simuleringar för att analysera deras beteenden samt få insikt om de underliggande fysikaliska processerna. Avhandlingen behandlar även tillämpningar av en elektriskt styrbar fiber för att filtrera radiofrekvenser. Ett ultrasmalt fasskiftat fiber Bragg gitter skrevs in i en fiber med interna elektroder och användes som ett svepande filter för att mäta modulationsfrekvensen på en optisk bärfrekvens. Ett liknande gitter användes inuti en laserkavitet för att generera två olika våglängder samtidigt. Dessa två våglängder användes sedan för att generera en svävningsfrekvens i mikrovågsbandet. De undersökta monolitiska fiberinterferometrarna och de aktivt styrbara fibrerna erbjuder en utmärkt byggsten inom så pass skiljda områden som Mikrovågsfotonik, Telekommunikation, Sensorer samt Höghastighets-omkopplare och bör kunna användas inom många olika tillämpningar i framtiden.
QC 20130226
Sergeeva, Anna. "Centrum chytré čtvrti Špitálka." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2021. http://www.nusl.cz/ntk/nusl-443698.
Full textChitti, Babu Surendra. "Development of tailored preform processing technology for net-shape manufacturing of large monolithic structures." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4861.
Full textThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on January 31, 2008) Vita. Includes bibliographical references.
Wehring, Markus, Jan-Henrik Smått, Mika Lindén, Frank Stallmach, and Jörg Kärger. "NMR studies on silica monoliths - diffusion in a hierarchical pore structure." Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-194114.
Full textWehring, Markus, Jan-Henrik Smått, Mika Lindén, Frank Stallmach, and Jörg Kärger. "NMR studies on silica monoliths - diffusion in a hierarchical pore structure." Diffusion fundamentals 6 (2007) 71, S. 1-2, 2007. https://ul.qucosa.de/id/qucosa%3A14251.
Full textRokotianskaia, Kseniia. "Facelift EDU." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2020. http://www.nusl.cz/ntk/nusl-414282.
Full textZhakupbekova, Rakhil. "Facelift EDU." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2020. http://www.nusl.cz/ntk/nusl-414302.
Full textKonishi, Junko. "Studies on functional TiO2 and ZrO2 monoliths with controlled porous structures." 京都大学 (Kyoto University), 2008. http://hdl.handle.net/2433/136298.
Full textGonçalves, Bianca Leopoldo. "Porous structures for the purification of biopharmaceuticals." Master's thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/12128.
Full textproject PTDC/EBB-BIO/118317/2010
Bombardier, Yan. "Prediction of the shape of fatigue cracks propagating in thick monolithic aluminium structures repaired using composite bonded doublers." Mémoire, Université de Sherbrooke, 2004. http://savoirs.usherbrooke.ca/handle/11143/1417.
Full textNovák, Jiří. "Novostavba Přírodovědecké fakulty Jihočeské univerzity v Českých Budějovicích - příprava realizace stavby." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2013. http://www.nusl.cz/ntk/nusl-226089.
Full textMalečková, Kateřina. "Architektonická studie sakrálního objektu Brno - Líšeň." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2020. http://www.nusl.cz/ntk/nusl-414268.
Full textSousa, Margarida Bucho Nunes de. "Smart macroporous structures for the purification of viral particles." Master's thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/12179.
Full textThe increasing application of viral particles in vaccination and gene-based therapies, has led to the development of alternative and improved purification processes. Traditional purification methods include chromatographic techniques, however the chromatographic matrices used present limitations specially when aimed at the purification of large molecules. This work presents the preparation of chitosan-based monoliths using clean processes and easy functionalization techniques intending to improve Adenovirus serotype 5 (Ad5) purification. Monoliths were prepared by blending chitosan (CHT) with glycidylmethacrylate (GMA) or poly(vinyl alcohol) (PVA), using two preparation techniques, freeze-drying and a scCO2 – assisted drying process, and were subsequently functionalized with Q ligands by three different methods. In addition, monoliths blended with magnetic nanoparticles were also prepared using the same strategies to confer them a controlled magnetic response. The monoliths produced were characterized in terms of ligand immobilization yield, and evaluated for Ad5 purification. Two types of monoliths showed potential: the CHT/PVA(50:50) prepared by freeze drying and functionalized by the alternative plasma technique (M2) and the CHT/PVA(50:50) 7% monolith prepared by scCO2 – assisted drying process and functionalized by the epoxyactivation technique (M1). The amount of ligand Q immobilized on the supports was monitored by titration assays, among which the CHT/PVA(50:50) 7% M2 prepared by scCO2 – assisted drying process exhibited the highest immobilization yield (91%). Among the results for Ad5 purification, the CHT/PVA(50:50)M2 and the CHT/PVA(50:50)7% M1 resulted in a 40% and 14% of the viral particles, respectively. Protein-binding assays were conducted using bovine serum albumin (BSA) and lysozyme, to evaluate the anionic-exchange capacity of the supports. The results make us believe in the potential of the produced monoliths to be applied in chromatographic techniques. However further improvements are necessary to enhance virus binding and recovery, to obtain an improved purification process.
project PTDC/EBB-BIO/118317/2010
Findejs, Lukáš. "Stavebně technologický projekt haly firmy PP&T." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2015. http://www.nusl.cz/ntk/nusl-227774.
Full text