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Journal articles on the topic 'Mousse poreuse'

Author: Grafiati

Published: 9 November 2024

Last updated: 31 July 2025

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1

Novo, Sergi, Leonardo Barrios, Elena Ibáñez, and Carme Nogués. "The Zona Pellucida Porosity: Three-Dimensional Reconstruction of Four Types of Mouse Oocyte Zona Pellucida Using a Dual Beam Microscope." Microscopy and Microanalysis 18, no. 6 (2012): 1442–49. http://dx.doi.org/10.1017/s1431927612013487.

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AbstractIn the last decade, the applicability of focus ion beam–field emission scanning electron microscopy (FIB-FESEM) in the biological field has begun to get relevance. Among the possibilities offered by FIB-FESEM, high-resolution three-dimensional (3D) reconstruction of biological structures is one of the most interesting. Using this tool, the 3D porosity of four different types of mouse oocyte zona pellucida (ZP) was analyzed. A surface analysis of the mouse oocyte ZP was first performed by SEM. Next, one oocyte per ZP type was selected, and an area of its ZP was completely milled, using

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Reynaud, A. "Filtration des inclusions par les matériaux poreux et les mousses en fonderie de fonte." Revue de Métallurgie 97, no. 1 (2000): 73–82. http://dx.doi.org/10.1051/metal/200097010073.

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Matsuoka, Shuji, Yasuyuki Ishii, Atsuhito Nakao, et al. "Establishment of a Therapeutic Anti-Pan HLA-Class II Monoclonal Antibody That Directly Induces Lymphoma Cell Death Via Large Pore Formation." Blood 124, no. 21 (2014): 5488. http://dx.doi.org/10.1182/blood.v124.21.5488.5488.

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Abstract To develop a new therapeutic monoclonal Antibody (mAb) for Hodgkin lymphoma (HL), we immunized a BALB/c mouse with live HL cell lines, alternating between two HL cell lines. After hybridization, we screened the hybridoma clones by assessing direct cytotoxicity against a HL cell line not used for immunization. We developed this strategy for establishing mAb to reduce the risk of obtaining clonotypic mAb specific for single HL cell line. A newly established mouse anti-human mAb (4713) triggered cytoskeleton-dependent, but complement- and caspase-independent, cell death in HL cell lines,

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Henry, Marianna M., and V. Ranga. "A Quantitative Study of the Development of Interalveolar Pores in the Postnatal Mouse." Experimental Lung Research 9, no. 3-4 (1985): 277–87. http://dx.doi.org/10.3109/01902148509057528.

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5

Bastacky, J., and J. Goerke. "Pores of Kohn are filled in normal lungs: low-temperature scanning electron microscopy." Journal of Applied Physiology 73, no. 1 (1992): 88–95. http://dx.doi.org/10.1152/jappl.1992.73.1.88.

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Interalveolar pores of Kohn, small uniform-sized epithelium-lined openings in alveolar walls of normal lung, have historically been demonstrated with electron-microscopic techniques that remove water. We show these pores to be present but almost invariably filled with material when water and surfactant are preserved in frozen hydrated lung examined with low-temperature scanning electron microscopy. In the normal mouse, 16 open empty pores per alveolus were found in instillation-fixed dried lung vs. less than 1 per alveolus in frozen hydrated lungs (P less than 0.001). In the normal rat, 13 por

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Ho, Han-Chen. "Redistribution of nuclear pores during formation of the redundant nuclear envelope in mouse spermatids." Journal of Anatomy 216, no. 4 (2010): 525–32. http://dx.doi.org/10.1111/j.1469-7580.2009.01204.x.

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Guo, Yuxuan, and Yixian Zheng. "Lamins position the nuclear pores and centrosomes by modulating dynein." Molecular Biology of the Cell 26, no. 19 (2015): 3379–89. http://dx.doi.org/10.1091/mbc.e15-07-0482.

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Lamins, the type V nuclear intermediate filament proteins, are reported to function in both interphase and mitosis. For example, lamin deletion in various cell types can lead to an uneven distribution of the nuclear pore complexes (NPCs) in the interphase nuclear envelope, whereas deletion of B-type lamins results in spindle orientation defects in mitotic neural progenitor cells. How lamins regulate these functions is unknown. Using mouse cells deleted of different combinations or all lamins, we show that lamins are required to prevent the aggregation of NPCs in the nuclear envelope near centr

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Xu, Tai-Min, Dong-Mei Wu, Neng Gao, et al. "Five New Species of Wood-Decaying Brown-Rot Fungi within Postiaceae (Polyporales, Basidiomycota) from Xinjiang, Northwest China." Journal of Fungi 10, no. 9 (2024): 655. http://dx.doi.org/10.3390/jof10090655.

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Brown-rot fungi are an important group of wood-decaying fungi, but there has been limited research on the species diversity of brown-rot fungi in Xinjiang, China. During an investigation of brown-rot fungi in Xinjiang, from July 2018 to July 2023, five new species belonging to the family Postiaceae were discovered based on morphological and molecular evidence. Amaropostia altaiensis is characterized by a conchate pileus, circular pores (5–8 per mm), and growing on Populus. Amaropostia tianshanensis is characterized by a flabelliform-to-conchate pileus, angular pores (5–6 per mm), and growing o

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Scherthan, Harry, Martin Jerratsch, Bibo Li, et al. "Mammalian Meiotic Telomeres: Protein Composition and Redistribution in Relation to Nuclear Pores." Molecular Biology of the Cell 11, no. 12 (2000): 4189–203. http://dx.doi.org/10.1091/mbc.11.12.4189.

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Mammalian telomeres consist of TTAGGG repeats, telomeric repeat binding factor (TRF), and other proteins, resulting in a protective structure at chromosome ends. Although structure and function of the somatic telomeric complex has been elucidated in some detail, the protein composition of mammalian meiotic telomeres is undetermined. Here we show, by indirect immunofluorescence (IF), that the meiotic telomere complex is similar to its somatic counterpart and contains significant amounts of TRF1, TRF2, and hRap1, while tankyrase, a poly-(ADP-ribose)polymerase at somatic telomeres and nuclear por

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Kim, Jeonghan, Rajeev Gupta, Luz P. Blanco, et al. "VDAC oligomers form mitochondrial pores to release mtDNA fragments and promote lupus-like disease." Science 366, no. 6472 (2019): 1531–36. http://dx.doi.org/10.1126/science.aav4011.

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Mitochondrial stress releases mitochondrial DNA (mtDNA) into the cytosol, thereby triggering the type Ι interferon (IFN) response. Mitochondrial outer membrane permeabilization, which is required for mtDNA release, has been extensively studied in apoptotic cells, but little is known about its role in live cells. We found that oxidatively stressed mitochondria release short mtDNA fragments via pores formed by the voltage-dependent anion channel (VDAC) oligomers in the mitochondrial outer membrane. Furthermore, the positively charged residues in the N-terminal domain of VDAC1 interact with mtDNA

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11

MATSUDA, Takeshi, Yoshifumi FUKUO, Harumichi SHINOHARA, Satoshi MORISAWA, and Toshio NAKATANI. "The Postnatal Development of the Mouse Pericardium; the Time and Mechanism of Formation of Pericardial Pores." Okajimas Folia Anatomica Japonica 67, no. 2-3 (1990): 115–20. http://dx.doi.org/10.2535/ofaj1936.67.2-3_115.

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12

Lin, Patricia W., Peter O. Simon, Andrew T. Gewirtz, et al. "Paneth Cell Cryptdins Actin Vitroas Apical Paracrine Regulators of the Innate Inflammatory Response." Journal of Biological Chemistry 279, no. 19 (2004): 19902–7. http://dx.doi.org/10.1074/jbc.m311821200.

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Intestinal-specific antimicrobial α-defensins, termed cryptdins, are secreted into the intestinal lumen by mouse Paneth cells in response to microbial pathogens. Cryptdins kill microbes by forming pores in their limiting membranes. The cryptdin isoforms 2 and 3 also can form anion-conductive pores in eukaryotic cell membranes, thus affecting cell physiology. Here, we find that when applied to apical membranes of the human intestinal cell line T84, cryptdin 3 (Cr3) induces secretion of the proinflammatory cytokine interleukin 8 (IL-8) in a dose-dependent manner. The induction of IL-8 secretion

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D'Cruz, Akshay A., Meghan Bliss-Moreau, Maria Ericcson, and Ben A. Croker. "Mlkl Pores Release Neutrophil Extracellular Traps in Necroptotic Neutrophils." Blood 126, no. 23 (2015): 2200. http://dx.doi.org/10.1182/blood.v126.23.2200.2200.

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Abstract Neutrophil extracellular traps (NETs) are networks of extracellular nuclear DNA and microbicidal proteins released from neutrophils in response to tissue damage and infection. Despite evidence of pathogenic roles for NETs in systemic lupus erythematosus, rheumatoid arthritis, diabetes, artherosclerosis and Alzheimer's disease, the major biochemical pathways controlling their formation remains poorly understood. Apoptosis does not contribute to NET formation but the role of regulated non-apoptotic cell death pathways such as necroptosis is not known. We have investigated the role of po

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Vautier, D., P. Chesne, C. Cunha, A. Calado, J. P. Renard, and M. Carmo-Fonseca. "Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos." Journal of Cell Science 114, no. 8 (2001): 1521–31. http://dx.doi.org/10.1242/jcs.114.8.1521.

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A unique feature of certain members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of proteins is that they shuttle continuously between nucleus and cytoplasm and their accumulation in the nucleus is transcription-dependent. An extensively characterised protein of this group is hnRNP A1. To date, most studies addressing the transcription-dependent transport of hnRNP A1 have been performed on cultured cell lines treated with transcription inhibitors. Here we have analysed the nucleocytoplasmic distribution of hnRNP A1 in early mouse embryos, where the haploid pronuclei remain tra

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Kadlečková, Markéta, Kateřina Skopalová, Barbora Ptošková, et al. "Hierarchically Structured Surfaces Prepared by Phase Separation: Tissue Mimicking Culture Substrate." International Journal of Molecular Sciences 23, no. 5 (2022): 2541. http://dx.doi.org/10.3390/ijms23052541.

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The pseudo 3D hierarchical structure mimicking in vivo microenvironment was prepared by phase separation on tissue culture plastic. For surface treatment, time-sequenced dosing of the solvent mixture with various concentrations of polymer component was used. The experiments showed that hierarchically structured surfaces with macro, meso and micro pores can be prepared with multi-step phase separation processes. Changes in polystyrene surface topography were characterized by atomic force microscopy, scanning electron microscopy and contact profilometry. The cell proliferation and changes in cel

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Rinde, Mia, Natalia Kupferschmidt, Muhammad Naeem Iqbal, et al. "Mesoporous silica with precisely controlled pores reduces food efficiency and suppresses weight gain in mice." Nanomedicine 15, no. 2 (2020): 131–44. http://dx.doi.org/10.2217/nnm-2019-0262.

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Aim: Obesity is a risk factor for cardiovascular disease and diabetes. We aimed to elucidate the effects of distinct mesoporous silica particles (MSPs) supplemented in food on metabolic parameters in obesity. Materials & methods: MSPs with precisely controlled pore size were synthesized, characterized and compared with a control in a C57Bl/6 mouse diet-induced obesity model, studying weight, adiposity, metabolic regulation and food efficiency. Results: The most effective MSPs reduced adipose tissue formation to 6.5 ± 0.5 g compared with 9.4 ± 1.2 g, leptin levels nearly halved from 32.8 ±

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Aglietti, Robin A., Alberto Estevez, Aaron Gupta, et al. "GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes." Proceedings of the National Academy of Sciences 113, no. 28 (2016): 7858–63. http://dx.doi.org/10.1073/pnas.1607769113.

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Gasdermin-D (GsdmD) is a critical mediator of innate immune defense because its cleavage by the inflammatory caspases 1, 4, 5, and 11 yields an N-terminal p30 fragment that induces pyroptosis, a death program important for the elimination of intracellular bacteria. Precisely how GsdmD p30 triggers pyroptosis has not been established. Here we show that human GsdmD p30 forms functional pores within membranes. When liberated from the corresponding C-terminal GsdmD p20 fragment in the presence of liposomes, GsdmD p30 localized to the lipid bilayer, whereas p20 remained in the aqueous environment.

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Hurt, E. C. "Targeting of a cytosolic protein to the nuclear periphery." Journal of Cell Biology 111, no. 6 (1990): 2829–37. http://dx.doi.org/10.1083/jcb.111.6.2829.

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The yeast nuclear envelope protein NSP1 is located at the nuclear pores and mediates its essential function via the carboxy-terminal domain. The passenger protein, cytosolic dihydrofolate reductase from mouse, was fused to the 220 residue long NSP1 carboxy-terminal domain. When expressed in yeast, this chimeric protein was tightly associated with nuclear structures and was localized at the nuclear periphery very similar to authentic NSP1. Furthermore, the DHFR-C-NSP1 fusion protein was able to complement a yeast mutant lacking a functional NSP1 gene showing that DHFR-C-NSP1 fulfils the same ba

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19

Kiradzhiyska, Denitsa, Nikolina Milcheva, Rositsa Mancheva, Tsvetelina Batsalova, Balik Dzhambazov, and Nikolay Zahariev. "Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications." Metals 12, no. 1 (2021): 51. http://dx.doi.org/10.3390/met12010051.

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The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al2O3 coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The mod

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Okazaki, M., Y. Tieliewuhan, and I. Hirata. "Osteoblast Behavior at the Surface of CO₃Ap-Collagen Sponges." Key Engineering Materials 309-311 (May 2006): 989–92. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.989.

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Carbonate apatite (CO3Ap) was synthesized at 60+1°C and pH 7.4+0.2, to develop a new biodegradable scaffold biomaterial. The synthetic CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures with different kinds of CO3Ap contents and porosity were frozen and dried in lyophilized into the sponges. CO3Ap-collagen mixtures were also lyophilized into sponges in a HAp frame ring with 0.5 mm pores. To examine the degree of cell invasion, mouse MC3T3-E1 cells were grown in αMEM with 10% heat-inactivated FBS in 96-well plates containing the CO3Ap-collagen sponges at 37°C in a 5

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Chaly, Nathalie, Gerald St. Aubin, and David L. Brown. "Ultrastructural localization of nuclear antigens during interphase in mouse 3T3 fibroblasts." Biochemistry and Cell Biology 67, no. 9 (1989): 563–74. http://dx.doi.org/10.1139/o89-088.

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Thin sections of mouse 3T3 fibroblast nuclei labelled by immunoperoxidase with anti-nuclear antibodies I1, PI1, PI2, anti-peripherin, -lamin, and -centromere have been examined in the electron microscope. Staining was compared with the corresponding immunofluorescence labelling patterns, and was correlated with nuclear ultrastructure in conventionally fixed and uranyl-lead stained samples and in unlabelled immunoperoxidase controls. Peripherin was detected at the nuclear rim in a band broader and more irregular than the lamins/lamina. The peripheral components of PI1 and PI2 appear to be local

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Matsuoka, Shuji, Hiromichi Tsurui, Masaaki Abe та ін. "A Monoclonal Antibody to the α2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model". Journal of Experimental Medicine 198, № 3 (2003): 497–503. http://dx.doi.org/10.1084/jem.20021301.

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We earlier found that a rat monoclonal antibody (mAb) RE2 can induce rapid death of murine activated, but not resting, lymphocytes and lymphocyte cell lines, in a complement-independent manner, a cell death differing from typical apoptosis or necrosis. We here found that this cell death is independent of pathways involving Fas, caspase, and phosphoinositide-3 kinase. With the advantage of producing human B cell line transfectants with stable expression of human/mouse xeno-chimeric MHC class I genes, we found that RE2 epitope resides on the murine class I α2 domain. However, the α3 domain plays

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Hamada, Masakazu, Anna Haeger, Karthik B. Jeganathan та ін. "Ran-dependent docking of importin-β to RanBP2/Nup358 filaments is essential for protein import and cell viability". Journal of Cell Biology 194, № 4 (2011): 597–612. http://dx.doi.org/10.1083/jcb.201102018.

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RanBP2/Nup358, the major component of the cytoplasmic filaments of the nuclear pore complex (NPC), is essential for mouse embryogenesis and is implicated in both macromolecular transport and mitosis, but its specific molecular functions are unknown. Using RanBP2 conditional knockout mouse embryonic fibroblasts and a series of mutant constructs, we show that transport, rather than mitotic, functions of RanBP2 are required for cell viability. Cre-mediated RanBP2 inactivation caused cell death with defects in M9- and classical nuclear localization signal (cNLS)–mediated protein import, nuclear ex

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Feng, Dian, Janice A. Nagy, John Hipp, Kathryn Pyne, Harold F. Dvorak, and Ann M. Dvorak. "Reinterpretation of endothelial cell gaps induced by vasoactive mediators in guinea-fig, mouse and rat: many are transcellular pores." Journal of Physiology 504, no. 3 (1997): 747–61. http://dx.doi.org/10.1111/j.1469-7793.1997.747bd.x.

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Okumura, Noriko, Takafumi Yoshikawa, Akitaka Nonomura, and Yoshinori Takakura. "Organ Regeneration in Porous Hydoroxyapatite." Key Engineering Materials 309-311 (May 2006): 1017–22. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.1017.

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HA has a high affinity for bone as well as various tissues. In the present study, we investigated an affinity for abdominal organs. Coralline hydroxyapatite ceramic (HA, cubic structure 4x4x4mm, Interpore 500) was used in this experiment. We made two incisions in the lower back of a 5-week-old male nude mouse, and implanted HA blocks. One was placed around the liver at the right side and another one was placed around the kidney at the left side. The organ fibrous capsule was not removed. At 6 weeks after implantation, mice were sacrificed under overanesthesia and HA blocks were retrieved and p

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Séméria, Yves, and Guy Vannier. "Les espèces continentales du phylum Tardigrada dans leurs rapports avec la porosphère. De la respiration aquatique à la respiration aérienne." Bulletin de la société linnéenne de Lyon 89, no. 1 (2020): 27–38. https://doi.org/10.3406/linly.2020.17986.

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Au cours de l’évolution, les Invertébrés comme les Tardigrades sont passés des habitats aquatiques aux habitats aériens à travers un milieu de transition représenté par les produits divisés de l’érosion des roches cristallines et sédimentaires, les sols, les humus, les matières organiques en décomposition, les plantes pionnières que sont les Mousses, les Lichens, les Hépatiques... qui forment le groupe des corps poreux composés de cavités minuscules et d’interstices avec une surface interne qui appartiennent au terme collectif de porosphère entre l’hydrosphère et l’atmosphère. Le processus de

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Zhang, Steven B., David Maguire, Mei Zhang, et al. "Mitochondrial DNA and Functional Investigations into the Radiosensitivity of Four Mouse Strains." International Journal of Cell Biology 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/850460.

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We investigated whether genetic radiosensitivity-related changes in mtDNA/nDNA ratios are significant to mitochondrial function and if a material effect on mtDNA content and function exists. BALB/c (radiosensitive), C57BL/6 (radioresistant), and F1 hybrid mouse strains were exposed to total body irradiation. Hepatic genomic DNA was extracted, and mitochondria were isolated. Mitochondrial oxygen consumption, ROS, and calcium-induced mitochondrial swelling were measured. Radiation influenced strain-specific survivalin vivo. F1 hybrid survival was influenced by maternal input. Changes in mitochon

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ZHANG, QIU-YUE, HONG-GAO LIU, GUANG-YU ZENG, and YU-CHENG DAI. "Polyporus miscanthi (Polyporaceae, Basidiomycota), a new polypore on Miscanthus (Poaceae) from Guangxi, South China." Phytotaxa 655, no. 3 (2024): 249–60. http://dx.doi.org/10.11646/phytotaxa.655.3.3.

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Polyporus is a widespread genus with highly variable morphological features and intricate phylogenetic relationships. Polyporus miscanthi, collected from Guangxi Autonomous Region, South China, is presented as a new species based on both morphological and phylogenetic analyses. It is characterized by annual, solitary, eccentrically stipitate basidiomata with irregularly circular, depressed center or infundibuliform pilei, yellowish to buff pileal surface with radially aligned stripes, white to cream pores, 4–5 per mm, a mouse gray to black stipe, cylindrical basidiospores measuring 9–11 × 3.5–

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Forysenkova, Anna A., Inna V. Fadeeva, Dina V. Deyneko, et al. "Polyvinylpyrrolidone—Alginate—Carbonate Hydroxyapatite Porous Composites for Dental Applications." Materials 16, no. 12 (2023): 4478. http://dx.doi.org/10.3390/ma16124478.

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An alternative approach for the currently used replacement therapy in dentistry is to apply materials that restore tooth tissue. Among them, composites, based on biopolymers with calcium phosphates, and cells can be applied. In the present work, a composite based on polyvinylpyrrolidone (PVP) and alginate (Alg) with carbonate hydroxyapatite (CHA) was prepared and characterized. The composite was investigated by X-ray diffraction, infrared spectroscopy, electron paramagnetic resonance (EPR) and scanning electron microscopy methods, and the microstructure, porosity, and swelling properties of th

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Petitto, Evelina, Jennifer K. Blackburn, M. Atiqur Rahman, and Yuri A. Ushkaryov. "The Dissociation of Latrophilin Fragments by Perfluorooctanoic Acid (PFOA) Inhibits LTXN4C-Induced Neurotransmitter Release." Toxins 17, no. 7 (2025): 359. https://doi.org/10.3390/toxins17070359.

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α-Latrotoxin stimulates neurotransmitter release by binding to a presynaptic receptor and then forming ion-permeable membrane pores and/or stimulating the receptor, latrophilin-1, or Adhesion G-protein-coupled receptor type L1 (ADGRL1). To avoid pore formation, we use the mutant α-latrotoxin (LTXN4C), which does not form pores and only acts through ADGRL1. ADGRL1 is cleaved into an N-terminal fragment (NTF) and a C-terminal fragment (CTF), which behave as independent cell-surface proteins, reassociating upon binding LTXN4C. We investigated the role of the NTF-CTF association in LTXN4C action,

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Zubova, Ganna, Hanna Melnyk, Iryna Zaets, et al. "Halochromic Bacterial Cellulose/Anthocyanins Hybrid Polymer Film with Wound-Healing Potential." Polymers 16, no. 16 (2024): 2327. http://dx.doi.org/10.3390/polym16162327.

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Polymer-based dressings deriving from natural biomaterials have advantages such as nontoxicity, biocompatibility, and mechanical stability, which are essential for efficient wound healing and microbial infection diagnostics. Here, we designed a prototype of an intelligent hydrogel dressing on the base of bacterial cellulose (BC) for monitoring wound microbial infection due to the uploaded natural pH dye-sensor, anthocyanins (ANC) of elderberry fruit (Sambucus nigra L.). The highest sensor responses to bacterial metabolites for ANC immobilized to BC were observed at pH 5.0 and 6.0. The detectio

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Singh, Mandakini B., Jesse A. White, Eric J. McKimm, Milena M. Milosevic, and Srdjan D. Antic. "Mechanisms of Spontaneous Electrical Activity in the Developing Cerebral Cortex—Mouse Subplate Zone." Cerebral Cortex 29, no. 8 (2018): 3363–79. http://dx.doi.org/10.1093/cercor/bhy205.

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Abstract Subplate (SP) neurons exhibit spontaneous plateau depolarizations mediated by connexin hemichannels. Postnatal (P1–P6) mice show identical voltage pattern and drug-sensitivity as observed in slices from human fetal cortex; indicating that the mouse is a useful model for studying the cellular physiology of the developing neocortex. In mouse SP neurons, spontaneous plateau depolarizations were insensitive to blockers of: synaptic transmission (glutamatergic, GABAergic, or glycinergic), pannexins (probenecid), or calcium channels (mibefradil, verapamil, diltiazem); while highly sensitive

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Popken, J., M. Dahlhoff, T. Guengoer, et al. "92 NUCLEAR INVAGINATIONS ADAPT TO RABBIT EARLY EMBRYONIC DEVELOPMENT." Reproduction, Fertility and Development 27, no. 1 (2015): 139. http://dx.doi.org/10.1071/rdv27n1ab92.

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Nuclear invaginations carrying nuclear pores may facilitate increased mRNA export and protein import to areas inside the nucleus remote from the nuclear border. In this study on rabbit embryos, we investigated whether large early embryonic nuclei and the increased import/export demands around major embryonic genome activation (MGA) at the 8-cell stage affected the quantity of nuclear invaginations carrying nuclear pores. To achieve this objective, we used super-resolution 3-dimensional structured illumination microscopy on 10 pronuclei or nuclei per stage of 23 in vivo-fertilized and in vitro-

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Fan, Hui, Junfeng Hui, Zhiguang Duan, et al. "Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering." BioMed Research International 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/652432.

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We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of73.6±2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Sub

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Ninivaggi, Marisa, Hilde Kelchtermans, Marijke J. Kuijpers, et al. "Whole blood thrombin generation in Bmal1-deficient mice." Thrombosis and Haemostasis 112, no. 08 (2014): 271–75. http://dx.doi.org/10.1160/th13-11-0910.

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SummaryThe Calibrated Automated Thrombogram (CAT) assay that measures thrombin generation (TG) in platelet-poor and -rich plasma, is increasingly being recognised as a more sensitive tool to determine the overall function of the haemostatic system. We developed a method enabling the measurement of TG in a small aliquot of blood. The objective was to validate this assay in mouse blood and to examine the rate and extent of TG in a mouse model of premature aging. TG was assayed in blood from 20– to 28-week-old brain and muscle ARNT-like protein-1 (Bmal1)-deficient (knockout, KO) mice and wild-typ

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Grigoriev, A. M., Yu B. Basok, A. D. Belova, et al. "Biological properties of macroporous cryostructurate based on extracellular matrix components." Russian Journal of Transplantology and Artificial Organs 25, no. 4 (2023): 109–20. http://dx.doi.org/10.15825/1995-1191-2023-4-109-120.

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Objective: to study the biological properties of macroporous cryostructurate from multicomponent concentrated collagen-containing solution (MCCS) as a promising matrix for the formation of cell- and tissue-engineered constructs.Materials and methods. A macroporous spongy carrier was obtained by cryostructuring of collagencontaining extract, prepared by acetic acid hydrolysis of chicken connective tissue (BIOMIR Service, Russian Federation). N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (Sigma-Aldrich, USA) was used to make the cryostructurate water insoluble. The micromorphology of the sponge

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Agapova, O. I., A. E. Efimov, M. M. Moisenovich, V. G. Bogush, and I. I. Agapov. "COMPARATIVE ANALYSIS OF THREE-DIMENSIONAL NANOSTRUCTURE OF POROUS BIOCOMPATIBLE SCAFFOLDS MADE OF RECOMBINANT SPIDROIN AND SILK FIBROIN FOR REGENERATIVE MEDICINE." Russian Journal of Transplantology and Artificial Organs 17, no. 2 (2015): 37–44. http://dx.doi.org/10.15825/1995-1191-2015-2-37-44.

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Aim.To perform a comparison of three-dimensional nanostructure of porous biocompatible scaffolds made of fibroinBombix moriand recombinant spidroin rS1/9.Materials and methods.Three-dimensional porous scaffolds were produced by salt leaching technique. The comparison of biological characteristics of the scaffolds shows that adhesion and proliferation of mouse fibroblastsin vitroon these two types of scaffolds do not differ significantly. Comparative experimentsin vivoshow that regeneration of bone tissue of rats is faster with implantation of recombinant spidroin scaffolds. Three-dimensional n

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Hodaei, Mohammad, and Andreas Mandelis. "Quantitative osteoporosis diagnosis of porous cancellous bone using poroelastodynamic modal analysis." Journal of the Acoustical Society of America 154, no. 5 (2023): 3101–24. http://dx.doi.org/10.1121/10.0022351.

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Osteoporosis is a skeletal disease characterized by reduced bone mass and microarchitectural deterioration, leading to increased fragility. This study presents a novel three-dimensional poroelastodynamic model for analyzing cancellous bone free vibration responses. The model incorporates the Navier-Stokes equations of linear elasticity and the Biot theory of porous media, allowing the investigation of osteoporosis-related changes. The analysis considers parameters like porosity, density, elasticity, Poisson ratio, and viscosity of bone marrow within the porous medium. Our findings indicate tha

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Solel, E., E. Brudvik, S. Afroz, et al. "P06.02.B GSDME-MEDIATED PYROPTOSIS IN GLIOBLASTOMA." Neuro-Oncology 25, Supplement_2 (2023): ii44—ii45. http://dx.doi.org/10.1093/neuonc/noad137.142.

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Abstract BACKGROUND Immunotherapeutic strategies have largely failed for glioblastoma , the most frequent primary brain tumor. Beyond apoptosis and autophagy, little is known about cell death mechanisms in glioblastoma, despite their potential impact on the immune microenvironment. Pyroptosis, unlike apoptosis, is an immunogenic and lytic type of programmed cell death, where gasdermin proteins form pores into the plasma membrane, releasing cell content to the tumor microenvironment. Gasdermin E (GSDME), often inactivated in cancers by mutations/low expression, is proteolytically cleaved by cas

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TAN, Jing-he, Zhong-Hua LIU, Wei REN, Hua NI, Xing-shen SUN, and Gui-xin HE. "The Role of Extracellular Ca2+ and Formation and Duration of Pores on the Oolemma in the Electrical Activation of Mouse Oocytes." Journal of Reproduction and Development 43, no. 4 (1997): 289–93. http://dx.doi.org/10.1262/jrd.43.289.

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Wardenburg, Juliane Bubeck, and Olaf Schneewind. "Vaccine protection against Staphylococcus aureus pneumonia." Journal of Experimental Medicine 205, no. 2 (2008): 287–94. http://dx.doi.org/10.1084/jem.20072208.

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Staphylococcus aureus pneumonia causes significant mortality in hospitalized or healthy individuals, and recent increases in morbidity are attributed to the rapid spread of methicillin-resistant S. aureus (MRSA) strains, which are often not susceptible to antibiotic therapy. α-Hemolysin (Hla), a secreted pore-forming toxin, is an essential virulence factor of MRSA in a mouse model of S. aureus pneumonia. We show that the level of Hla expression by independent S. aureus strains directly correlates with their virulence. Active immunization with a mutant form of Hla (HlaH35L), which cannot form p

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Seki, Shinsuke, Keisuke Edashige, Sakiko Wada, and Peter Mazur. "Effect of the expression of aquaporins 1 and 3 in mouse oocytes and compacted eight-cell embryos on the nucleation temperature for intracellular ice formation." REPRODUCTION 142, no. 4 (2011): 505–15. http://dx.doi.org/10.1530/rep-10-0538.

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The occurrence of intracellular ice formation (IIF) is the most important factor determining whether cells survive a cryopreservation procedure. What is not clear is the mechanism or route by which an external ice crystal can traverse the plasma membrane and cause the heterogeneous nucleation of the supercooled solution within the cell. We have hypothesized that one route is through preexisting pores in aquaporin (AQP) proteins that span the plasma membranes of many cell types. Since the plasma membrane of mature mouse oocytes expresses little AQP, we compared the ice nucleation temperature of

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OH, Seunguk, Rick Odland, Scott R. Wilson, et al. "Improved distribution of small molecules and viral vectors in the murine brain using a hollow fiber catheter." Journal of Neurosurgery 107, no. 3 (2007): 568–77. http://dx.doi.org/10.3171/jns-07/09/0568.

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Object A hollow fiber catheter was developed to improve the distribution of drugs administered via direct infusion into the central nervous system (CNS). It is a porous catheter that significantly increases the surface area of brain tissue into which a drug is infused. Methods Dye was infused into the mouse brain through convection-enhanced delivery (CED) using a 28-gauge needle compared with a 3-mm-long hollow fiber catheter. To determine whether a hollow fiber catheter could increase the distribution of gene therapy vectors, a recombinant adenovirus expressing the firefly luciferase reporter

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Liu, C. C., P. M. Persechini, and J. D. Young. "Expression and characterization of functionally active recombinant perforin produced in insect cells." Journal of Immunology 156, no. 9 (1996): 3292–300. http://dx.doi.org/10.4049/jimmunol.156.9.3292.

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Abstract A key cytolytic mediator used by killer lymphocytes, perforin (also known as pore-forming protein or cytolysin), has been shown to be capable of undergoing polymerization to form pores in cell membranes and cause osmotic lysis of target cells. Although perforin has been purified from killer lymphocytes and the coding gene has been cloned and sequenced, information concerning the domain structure of the perforin molecule has remained scarce. To overcome the difficulty in obtaining sufficient amounts of perforin and to further assess the functional relevance of the N-terminal portion of

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Arzumanyan, V. G., T. A. Artemeva, and A. M. Iksanova. "ANTIFUNGAL ACTIVITY OF HUMAN AND SOME MAMMALS SERA." Journal of microbiology epidemiology immunobiology 1, no. 1 (2019): 17–22. http://dx.doi.org/10.36233/0372-9311-2019-1-17-22.

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Aim. Estimation of activity of native human serum and its antimicrobial peptides fraction against clinically important yeasts and comparison with the activity of some mammals sera. Materials and methods. Pooled samples of human, bovine, rabbit and mouse sera and collection strains of yeasts Candida albicans, Rhodotorula mucilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cutaneum, Saccharomyces cerevisiae were used in the study. Antimicrobial peptides fractions (AMP) were obtained by filtration through molecular filters with 100 kDa pores. Activity of se

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Qiu, Yujia, Elma Sakinatus Sajidah, Sota Kondo, et al. "An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy." Cells 13, no. 3 (2024): 279. http://dx.doi.org/10.3390/cells13030279.

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Nuclear pore complexes (NPCs) on the nuclear membrane surface have a crucial function in controlling the movement of small molecules and macromolecules between the cell nucleus and cytoplasm through their intricate core channel resembling a spiderweb with several layers. Currently, there are few methods available to accurately measure the dynamics of nuclear pores on the nuclear membranes at the nanoscale. The limitation of traditional optical imaging is due to diffraction, which prevents achieving the required resolution for observing a diverse array of organelles and proteins within cells. S

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Leung, Jennifer, Michael Chang, Richard E. Moore, Jargalsaikhan Dagvadorj, Fayyaz S. Sutterwala та Suzanne L. Cassel. "Gasdermin D and Gasdermin E Are Dispensable for Silica-Mediated IL-1β Secretion from Mouse Macrophages". ImmunoHorizons 8, № 9 (2024): 679–87. http://dx.doi.org/10.4049/immunohorizons.2400019.

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Abstract Silica crystals activate the NLRP3 inflammasome in macrophages, resulting in the caspase-1–dependent secretion of the proinflammatory cytokine IL-1β. Caspase-1–mediated cleavage of gasdermin D (GSDMD) triggers the formation of GSDMD pores, which drive pyroptotic cell death and facilitate the rapid release of IL-1β. However, the role of GSDMD in silica-induced lung injury is unclear. In this study, we show that although silica-induced lung injury is dependent on the inflammasome adaptor ASC and IL-1R1 signaling, GSDMD is dispensable for acute lung injury. Although the early rapid secre

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Predescu, D., and G. E. Palade. "Plasmalemmal vesicles represent the large pore system of continuous microvascular endothelium." American Journal of Physiology-Heart and Circulatory Physiology 265, no. 2 (1993): H725—H733. http://dx.doi.org/10.1152/ajpheart.1993.265.2.h725.

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In the capillary physiology literature, molecules and particles larger than 10 nm are assumed to leave the plasma mostly through large pores located at the level of intercellular junctions in microvessels lined with a continuous endothelium. In morphological studies of similar microvessels, outgoing particles > 10 nm were detected in endothelial plasmalemmal vesicles not in intercellular junctions. Because the probes may not be found in transit through the junctions because they may be swept away by strong currents generated by Starling forces, we have examined a large number of junctions i

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Matsuura, T., M. Kawada, S. Hasumura, et al. "High Density Culture of Immortalized Liver Endothelial Cells in the Radial-flow Bioreactor in the Development of an Artificial Liver." International Journal of Artificial Organs 21, no. 4 (1998): 229–34. http://dx.doi.org/10.1177/039139889802100410.

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Liver endothelial cells are important components of the tissue along the hepatic sinusoid. They are responsible for microcirculation in the liver and scavenger functions. It would therefore be important to include these cells in any hybrid type of artificial liver in addition to hepatocytes. However, it is difficult to culture these cells in vitro. The development of a liver endothelial cell line, which maintains the characteristics of the primary culture, would thus be of great benefit in the development of an artificial liver. In the present study we established immortalized liver endothelia

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Dean, Kristin T., Bockgie Jung, and Buka Samten. "Identification of N-formylated peptides as the neutrophilic chemotactic factors of Mycobacterium tuberculosis." Journal of Immunology 204, no. 1_Supplement (2020): 148.19. http://dx.doi.org/10.4049/jimmunol.204.supp.148.19.

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Abstract Since tuberculosis (TB) remains a major cause of human death, it is urgent to understand the pathology of TB. Lung neutrophil infiltration initiates granuloma formation and is associated with disease severity. Though several cytokines and chemokines are associated with neutrophil infiltration in chronic inflammation, neutrophil chemotactic factors of Mycobacterium tuberculosis (Mtb) remain unexplored. Thus, we performed neutrophil chemotactic assays by culturing neutrophils of healthy human blood samples and mouse bone marrow in the upper chamber of a transwell plate with 5–8 μm pores

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