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1
Murthy Kumar, Karthikeyan. "Views on antioxidant mouth washes as adjunct in periodontal therapy." Bioinformation 16, no. 12 (December 31, 2020): 1069–79. http://dx.doi.org/10.6026/973206300161069.
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Clinical decision is often difficult with chlorhexidine mouthwash. The use of antioxidant mouthwashes for the treatment of periodontal disease is in practise. Therefore, it is of interest to collect gleaned information on Antioxidant mouthwashes as periodontal therapy from known literature. Improvement in treatment using antioxidant mouthwashes is reported in several studies. The mouthwash with antioxidants has similar anti-gingivitis, antiplaque and antimicrobial effects as that of chlorhexidine mouthwash.
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Murthy Kumar, Karthikeyan. "Views on antioxidant mouth washes as adjunct in periodontal therapy." Bioinformation 16, no. 12 (December 31, 2020): 1069–79. http://dx.doi.org/10.6026/973206300161069.
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Clinical decision is often difficult with chlorhexidine mouthwash. The use of antioxidant mouthwashes for the treatment of periodontal disease is in practise. Therefore, it is of interest to collect gleaned information on Antioxidant mouthwashes as periodontal therapy from known literature. Improvement in treatment using antioxidant mouthwashes is reported in several studies. The mouthwash with antioxidants has similar anti-gingivitis, antiplaque and antimicrobial effects as that of chlorhexidine mouthwash.
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Selvakumar, Renuka, and Muralidharan Np. "COMPARISON IN BENEFITS OF HERBAL MOUTHWASHES WITH CHLORHEXIDINE MOUTHWASH: A REVIEW." Asian Journal of Pharmaceutical and Clinical Research 10, no. 2 (February 1, 2017): 3. http://dx.doi.org/10.22159/ajpcr.2017.v10i2.13304.
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Objective: To discuss the benefits of herbal mouthwashes with the standard chlorhexidine mouthwash. This review is conducted to explore the benefits of herbal mouthwashes.Methods: Many herbal extracts are now available as mouthwash for maintaining the good oral hygiene. Plaque accumulation and increase in oral micro- organisms are the main factors for poor oral hygiene. Herbal extracts such as German chamomile, Terminalia chebula, Aloe vera, Green tea, peppermint satva, turmeric, neem, triphala, pomegranate extracts, guava extract, propolis, alum, darim leaves, mulethi, etc., are similar to chlorhexidine in plaque control and gingivitis reduction. Many herbal mouthwashes contain herbs with anti-microbial property such as neem, yavani satva, nagavalli, Gandhapura taila, pilu, Bibhitaka, Ocimum, Echinacea, Chameli leaves, etc. Many herbs are with anti-inflammatory and anti-oxidant property such as neem, clove, triphala (combination of amalaki, haritaki, and vibhitaki), tulsi, grapefruit, celery, licorice, katha, spearmint, and chamomile essential oil. Some herbal mouthwash with chamomile extract kills some skin pathogens such as staphylococcus and Candida species. Mixture of Staphysagria, Chamomilla, Echinacea, Plantago, Ocimum, and Cistus extracts used as mouth wash which was is better than chlorhexidine in reducing salivary mutans streptococcicount. Hence usage of herbal mouthwash will enhance the oral hygiene comparatively with chlorhexidine mouthwash without any adverse effects. Result: Though herbal mouthwashes has the ability to maintain good oral hygiene on daily basis, but still it is less effective than chlorhexidine mouthwash during treatments like gingivitis, periodontitis, trauma, etc. Conclusion: Besides the disadvantages, chlorhexidine mouthwash plays effective role during dental treatments on short term usage. Herbal mouthwashes are suitable for maintaining good oral prophylaxis. Many programs have to be conducted to make them aware about mouthwashes in their oral hygiene.Keywords: Chlorhexidine, Herbal, Mouthwash, Oral hygiene, Prophylaxis.
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Yaghini, Jaber, Narges Naghsh, Sayed Mohsen Sadeghi, and Samaneh Soltani. "Gingival Inflammatory Indices and Dental Stain Index after Using Aloe Vera-Green Tea Mouthwash, Matrica Mouthwash, or 0.2% Chlorhexidine Mouthwash Compared with Placebo in Patients with Gingival Inflammation." Open Dentistry Journal 13, no. 1 (June 30, 2019): 214–20. http://dx.doi.org/10.2174/1874210601913010214.
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Introduction: The purpose of this study was to evaluate and compare the effects of composition of aloe vera-green tea, matrica, and chlorhexidine on gingival inflammatory indices and dental stain index. Materials & Methods: In this double-blinded placebo-controlled clinical trial, anti-inflammatory, anti-plaque formation, and dental staining effects of two herbal mouthwashes, including aloe vera-green tea and matrica in comparison with chlorhexidine in 60 patients with plaque induced gingivitis referring to Isfahan University of Medical Sciences, School of Dentistry were evaluated. The indices evaluated in this study were plaque index (Silness and Loe), gingival index (Loe and Silness), bleeding on probing index (Ainamo and Bay) and dental stain index (Lobene stain index). They were evaluated on the first day of using mouthwashes and two weeks later. The obtained data were analyzed using SPSS software version 22. One-way ANOVA, Tukey post hoc, and paired t and Chi-square tests (α=0.05) were used as appropriate. Results: There was no significant difference between the four groups in terms of the mean values of plaque index, gingival index, BOP index, and stain index before the application of mouthwash. However, after mouthwash application, the mean values of indices were significantly different between the four groups. Aloe vera-green tea and chlorhexidine mouthwashes reduced plaque index, gingival index, and bleeding on probing index significantly and there was no significant difference between these two mouthwashes (P>0.05). The effect of matrica mouthwash on plaque index and gingival index was significantly lower than aloe vera-green tea and chlorhexidine (P<0.05). The mean reduction in BOP index was not significantly different between the aloe vera-green tea, chlorhexidine, and matrica groups. Regarding dental stain index, both herbal mouthwashes caused significantly lower dental stain in comparison with chlorhexidine (P<0.05). Conclusion: The results of the present study show that aloe vera-green tea mouthwash may be an effective mouthwash owing to its antiplaque and anti-inflammatory properties and may be an ideal substitute for chlorhexidine. Clinical Trial: The clinical trial code: IR.MUI.REC.1395.3.573
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Ravikumar, Chandini, Radhika Arjunkumar, and Gopinath Prakasam. "COMPARISON OF ANTIFUNGAL EFFECTS OF COMMERCIALLY AVAILABLE HERBAL MOUTHWASHES AND CHLORHEXIDINE AGAINST CANDIDA ALBICANS IN DIABETIC PATIENTS: AN IN VITRO STUDY." Asian Journal of Pharmaceutical and Clinical Research 9, no. 5 (September 1, 2016): 214. http://dx.doi.org/10.22159/ajpcr.2016.v9i5.13275.
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ABSTRACTObjective: Candida albicans is a part of the normal flora of the mouth in diabetes mellitus (DM) patients. Periodontitis is one of the main complicationsin diabetic patients. Mechanical and chemical plaque control are the most productive methods in preventing periodontal diseases in the oral cavity.The objective of this study is to compare the in vitro effect of herbal mouthwashes and chlorhexidine (CHX) against C. albicans.Methods: Saliva samples were obtained from diabetic patients reporting for treatment to Saveetha medical college. C. albicans was cultured from thesalivary sample. A yeast suspension was made by sub culturing the C. albicans. The mouthwashes used in the study are HiOra regular (0.2%), HiOrasensitive (0.5%), and clohex plus (CHX gluconate 0.02%) mouthwashes. The fungal suspension was spread on Sabouraud’s dextrose agar (SDA) plateswith a sterile swab. Subsequently, wells of 6 mm in diameter were made with a suitable distance using sterile cork borer on pre-inoculated agar platesand filled with 100 µl of each mouthwashes. From the zones of inhibition seen, antimicrobial activity was expressed in terms of average diameter ofthe zones of inhibition measured.Results: Using HiOra regular mouthwash, 13/18 (72%) wells were found to show zone of inhibition ≥20 mm. In HiOra sensitive mouthwash, only9/18 (50%) showed inhibition zone ≥20 mm. With effect of regular CHX mouthwash, none of the strains showed the zone of inhibition to be ≥20 mm.Most of the strains responded well with all the three mouthwashes.Conclusion: Among the 2 herbal mouthwashes, HiOra regular mouthwash was most effective in inhibiting the candidal growth when compared tothe HiOra sensitive. HiOra regular mouthwash still showed better inhibitory actions when compared to the regular CHX mouthwash and the candidalspecies showed increased sensitivity to it.Keywords: Candida albicans, Chlorhexidine mouthwash, Diabetes mellitus, Herbal mouthwashes, Zone of inhibition.
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Preethikaa Guruprasath, Ravishankar PL, Prem Blaisie Rajula M, Sunanda Rao K, Padmaja Vangipuram, and Visithiriyan G. "Evaluation of antimicrobial efficacy of Chlorhexidine, Listerine, and herbal mouthwashes in decontamination of toothbrushes-An invitro study." International Journal of Research in Pharmaceutical Sciences 11, no. 4 (December 21, 2020): 7366–69. http://dx.doi.org/10.26452/ijrps.v11i4.4597.
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Toothbrushing and toothbrushes play an important role in oral hygiene. Disinfection, storage and changing of toothbrushes at regular intervals are essential. The aim of the present study is to evaluate the invitro efficacy of Chlorhexidine, Listerine ® and herbal mouthwash (Himalaya Oro-TTM) against microorganisms as toothbrush disinfectants. A total of 20 participants were recruited. Brand new identical toothbrushes were given to each participant and they were asked to brush their teeth twice daily. They were instructed to use tap water to rinse the used toothbrushes. The toothbrushes were collected after a week. The bristles of each toothbrush were cut and kept in separate vials containing transport media. Then, they were sent for microbiological evaluation. The number of colony-forming units were measured. The vials containing bristles were divided into four groups, each group containing 5 vials: Group I: Chlorhexidine mouthwash; Group II: Listerine mouthwash; Group III: Herbal mouthwash; Group IV: Distilled water. The bristles were immersed in test-tube containing respective mouthwashes for one hour. And, colony-forming units were measured. In all three test groups, there was a significant difference in the reduction of microbes after sanitization with mouthwashes. The highest reduction was seen in group I and the least reduction was group IV. Disinfection of toothbrush may prevent the occurrence of oral infections. Chlorhexidine mouthwash was found to be more effective than other mouthwashes as a disinfecting agent in reducing the microbial load.
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Ramamurthy, Jaiganesh, and Visha Mg. "COMPARISON OF EFFECT OF HIORA MOUTHWASH VERSUS CHLORHEXIDINE MOUTHWASH IN GINGIVITIS PATIENTS: A CLINICAL TRIAL." Asian Journal of Pharmaceutical and Clinical Research 11, no. 7 (July 7, 2018): 84. http://dx.doi.org/10.22159/ajpcr.2018.v11i7.24783.
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Objective: The aim and objective of this study was to evaluate the effect of Hiora mouthwash versus Chlorhexidine mouthwash for the treatment of gingivitis.Methods: The study population was comprised 30 gingivitis patients. The patients were randomly categorized into two groups of 15 patients in each group. Group A was given Chlorhexidine mouthwash and Group B was given Hiora mouthwash. Pre-operative measurements such as plaque index (PI), gingival index (GI), and probing depth (PD), and loss of attachment (LA) were measured. Oral prophylaxis followed by the prescription of mouthwash was done. The patients were recalled for a review after 15 days, and post-operative measurements were recorded.Results: From the statistical analysis in the experimental Group A and Group B, the mean values of PD, LA, GI, and PI were found to be significantly lower in the post-operative period than the pre-operative mean values. At the end of 15 days, almost comparable reduction in the amount of plaque and gingivitis was found in both Group A and Group B. Hence, the differences in efficacy of these two mouthwashes were non-significant.Conclusion: Hiora and Chlorhexidine mouthwashes were equally effective in the treatment of gingivitis.
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Sharma, Shivalal, C. Saimbi, Bandana Koirala, and Rakesh Shukla. "Effect of Various Mouthwashes on the Levels of Interleukin-2 and Interferon-γ in Chronic Gingivitis." Journal of Clinical Pediatric Dentistry 32, no. 2 (December 1, 2007): 111–14. http://dx.doi.org/10.17796/jcpd.32.2.u01p135561161476.
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The aim of this double blind study was to evaluate the effect of various mouthwashes: Chlorhexidine, Essential oil, Azadirachta indica (Neem) extract, and Povidone iodine on gingival tissue interleukin-2 (IL-2) and interferon-γ (IFN-γ) levels in patients with chronic gingivitis. A total of 80 patients (42 boys, 38 girls; mean age 16.0 ± 1.8 years) were included in this study. Patients were randomly assigned into four groups of 20 each: Group I- Azadirachta indica (Neem) extract, Group II - Essential oil, Group III - Povidone iodine, and Group IV - Chlorhexidine. They were instructed to use these mouthwashes for two weeks. Plaque and gingival indices scores, and IL-2 and IFN-γ levels in the gingival tissues were measured at baseline and after two weeks of mouthwash use. Results showed the reduction of plaque and gingival indices, and IL-2 and IFN-γ level with Chlorhexidine, Essential oil, and Povidone iodine, which were found to be statistically significant. Although Neem reduced the level of plaque and gingival indices, and IL-2 and IFN-γ to a certain level, it was not statistically significant. Therefore, Chlorhexidine, Essential oil, and Povidone iodine mouthwashes can be used as an adjunct to oral prophylaxis in reducing pro-inflammatory cytokines, IL-2 and IFN-γ in patients with chronic gingivitis.
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Sabarathinam, Jembulingam, Deepak Nallaswamy, Deepak Nallaswamy, Muralidharan Np, and Muralidharan Np. "COMPARISON OF HERBAL MOUTHWASH WITH COMMERCIALLY AVAILABLE 0.2% CHLORHEXIDINE AND 2% BETADINE MOUTHWASHES IN PATIENTS AFTER STAGE-1 IMPLANT SURGERY." Asian Journal of Pharmaceutical and Clinical Research 10, no. 12 (December 1, 2017): 56. http://dx.doi.org/10.22159/ajpcr.2017.v10i12.17786.
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Objective: The objective of the current study is to assess the antibacterial efficiency of an herbal mouthwash (clove and neem) against 0.2% chlorhexidine and 2% betadone mouthwash in patients who have undergone Stage-1 implant surgery.Methods: 30 patients undergoing implant surgery (Stage-1) were divided into 3 groups and were given 3 different mouthwashes. The patient was recalled after 15 days. Swab samples from the site of implant were taken after 15 days and cultured. The results were tabulated.Results: 0.2% chlorhexidine and 2% bernadine were found to have better antibacterial efficiency than herbal mouthwash (p>0.05).Conclusion: The herbal mouthwash consisting of neem and clove was not efficient in killing microbes immediately after Stage-1 implant surgery when compared to 0.2% chlorhexidine and 2% bernadine.
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Porwal, Surbhi, Aditi Mathur, Neema Shetty, Balaji Manohar, Barkha Makhijani, and Rohit Mundra. "Comparative Evaluation of the Effect of Chlorhexidine Gluconate, Raw Propolis and Hydrogen Peroxide on Dental Plaque and Gingival Inflammation." Journal of Nepalese Society of Periodontology and Oral Implantology 2, no. 1 (June 4, 2018): 14–19. http://dx.doi.org/10.3126/jnspoi.v2i1.23603.
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Background: Plaque is the soft deposit that forms the biofilm consisting of microorganisms adhering to the tooth surface and is proved beyond doubt to be the initiator of gingival and periodontal disease. Plaque control is the mainstay in periodontal disease.
Aim: To compare the effect of 0.2% chlorhexidine gluconate, raw propolis and 3% hydrogen peroxide mouthwash on dental plaque and gingival inflammation.
Materials and Methods: Thirty subjects in the age group of 20-40 years were enrolled in the study. Plaque index and Modified Gingival Index were recorded at baseline and oral prophylaxis was performed. Subjects were then randomly divided into three groups (n=10) and were asked to rinse with 10ml mouthwash twice daily for 15 days. Group I received 0.2% chlorhexidine gluconate mouth wash, Group II raw propolis diluted with distilled water (1:1), and Group III 3% hydrogen peroxide (1:1) mouthwash. Subjects were recalled on 7 day and 28 day for re-evaluation and recording plaque index and modified gingival index. Statistical analysis was done to evaluate the efficacy of all the three mouthwashes.
Results: The results showed that all the three mouthwashes were effective in reducing plaque and gingival inflammation. Chlorhexidine gluconate 0.2% showed to be the most effective in reduction of dental plaque. Propolis was observed to be the most effective in reducing gingival inflammation over a period of 28 days.
Conclusion: Propolis can be effectively used as a mouthwash without any potential side effects as an alternative to chlorhexidine mouthwash in reducing gingival inflammation.
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Sikka, Geetanjali, and KT Chandrashekar. "Comparative Anti-plaque and Anti-gingivitis Efficacy of Two Commercially Available Mouthwashes - 4 Weeks Clinical Study." Journal of Oral Health and Community Dentistry 5, no. 3 (2011): 110–12. http://dx.doi.org/10.5005/johcd-5-3-110.
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ABSTRACT Therapeutic mouthwashes are often recommended as an adjunct to mechanical plaque control for prevention of plaque formation and maintain gingival health. The present clinical study was for the duration of four weeks and focused on two specific mouthwashes: HEXIDINE (0.2% chlorhexidine) and SEFDENT (a dual action mouthrinse with clove oil and sorbitol, sol isopropyl myristate, sodium saccharin) both claims to be effective in the treatment of gingivitis. HEXIDINE (Chlorhexidine) has long-standing research to substantiate its safety and efficacy whereas the SEFDENT mouthwash used in this study is a new mouthwash with many components in it. Subjects were randomly allocated into either the test group (n = 10,Sefdent) or the control group (n= 10,Hexidine) Plaque levels were measured using the Turesky's modification of the Quigley & Hein Plaque Index (PI), and gingivitis was evaluated and recorded with the Gingival Index (Loe and Silness 1963) (GI) at 0 day, 15th days and 30th day. All Subjects were instructed with oral hygiene instructions. First scaling and root planning was performed on all subjects and then they were asked to rinse twice daily with one of the mouthwashes (HEXIDINE or SEFDENT). Both the mouth-rinses studies turned out to be statistically significant but control group (HEXIDINE) showed significant improvement in plaque index, gingival scores, when compared to test group (SEFDENT). This shows that chlorhexidine remains a gold standard in improving the gingival status.
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Amer, Ibtesam O., Raja M. Moman, Mustafa M. Gebreil, and Mohamed R. Alsagher. "GROWTH INHIBITORY EFFECT OF CHLORHEXIDINE AND HEXETIDINE CONTAINING-MOUTHWASHES AGAINST ORAL BACTERIAL ISOLATES." Scientific Journal of Applied Sciences of Sabratha University 3, no. 2 (September 27, 2020): 91–102. http://dx.doi.org/10.47891/sabujas.v3i2.91-102.
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Background
Frequent use of mouthwash (MW) is one of the most effective methods used to prevent oral bacterial infections and to assist individuals in their efforts to achieve and maintain better oral health. Using a MW containing antibacterial agents would be a simple way to prevent growth and multiplication of pathogenic organisms in oral cavity causing dental caries and other mouth diseases. Chlorhexidine (CHX) and Hexetidine (HX) have been proposed as potent biocides against oral bacteria.
Objective
The present study was performed to investigate oral bacteria growth inhibition when using any of four mouthwashes that are commercially available in the Libyan market and contain either CHX, (Zordy land Oraxin), or HX, (Hextril and Givalex), and to clarify whether CHX and HX were suitable and safe biocides that can be included in mouthwash products.
Materials and Methods
Sixty adult (45 females and 15 males) volunteers had been chosen and divided into four groups and their saliva samples were assessed for microbial count at the beginning and the end of two weeks of treatment, during which they rinsed with 15ml of mouthwashes for 30 seconds twice a day (morning and evening) in addition to their usual oral hygiene procedures. The antibacterial activity of mouthwashes was assayed by cell viable count technique and cell diffusibility measurement.
Results
The results showed wide variations in the effectiveness of mouthwashes; those containing CHX were more effective (P ≤0.05) than formulations containing HX on oral microbial count. The main findings of the present study were that Zordyl, Oraxinand Hextril exerted high effects on the salivary microbiota, causing 90%, 60% and 34% reduction in salivary bacterial counts respectively. 25% reduction was observed for Givalex. On the other hand, the zone of inhibition test showed that Zordyl and Oraxin had large zone inhibitory effects, while Hextril and Givalex, were less effective on some bacterial species.
Conclusion
It can be concluded that twice daily use of CHX mouthwash (CHX-MW) or HX mouthwash (HX-MW) reduces oral bacterial load counts in healthy subjects when used as an adjunct to their normal oral hygiene procedures. This also suggests that inhibitory power of mouth washes containing CHX is greater on oral bacteria than mouthwashes containing HX.
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Moeintaghavi, Amir, Hamidreza Arab, Mehrangiz Khajekaramodini, Rohollah Hosseini, Hossein Danesteh, and Hamed Niknami. "In vitro Antimicrobial Comparison of Chlorhexidine, Persica Mouthwash and Miswak Extract." Journal of Contemporary Dental Practice 13, no. 2 (2012): 147–52. http://dx.doi.org/10.5005/jp-journals-10024-1111.
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ABSTRACT Aim Herbal mouthwashes, such as persica (Salvadora persica, mint and yarrow extracts) and miswak extract have been shown to decrease gingival inflammation and plaque accumulation. The aim of this study was to compare the antimicrobial activities of persica and miswak extract with the conventional mouthwash chlorhexidine against Streptococcus salivarius, Streptococcus sanguis, Lactobacillus vulgaris and Candida albicans. Materials and methods In this in vitro study, blood-agar culture (Merk, Germany) was used to grow the streptococcus strains, saburd-dextrose culture (Merk, Germany) was used to grow C. albicans and MRS-agar was used to grow L. vulgaris. Various concentrations of these substances (0.1, 0.05 and 0.025% of miswak extract, 0.1, 0.05, 0.025 and 0.0125% of persica, 0.2, 0.1, 0.05 and 0.025% of chlorhexidine) were added to paper disks, separately, inserted into culture plates and transferred into the incubator. The inhibition zone around each disk was measured after 24 hours and the data was analyzed by the Kruskal-Wallis test. Results Chlorhexidine possessed antibacterial activity at all concentrations tested. It was more effective than persica and miswak at all concentrations on S. salivarius (p = 0.022 for 0.1%, 0.009 for 0.05 and 0.025%). It had greater effect than the other two tested material on S.sanguis only at concentration 0.01%. Chlorhexidine was the most effective against S.salivarius; persica was the most effective against Lactobacillus (p = 0.005) and the least effective against S. salivarius; and miswak extract was the most effective against S. salivarius and S. sanguis at concentrations 0.1 and 0.05% (p = 0.005) and ineffective against L. vulgaris. None of these mouthwashes were effective against C. albicans. Conclusion This study revealed that chlorhexidine remains the gold standard as an antimicrobial agent, although herbalbased mouthwashes do have marginal antimicrobial activities. It is necessary to conduct more clinical and microbiological studies focusing on periodontal pathogens and anaerobic microorganisms. Clinical significance Mechanical plaque control is the main way for periodontal disease prevention and mouthrinses are used to improve its efficacy. Based on the results of this study, chlorhexidine has the most antibacterial effect and although persica mouthwash and miswak are routinely used in some Asian countries their antibacterial efficacies are suspected. How to cite this article Moeintaghavi A, Arab H, Khajekaramodini M, Hosseini R, Danesteh H, Niknami H. In vitro Antimicrobial Comparison of Chlorhexidine, Persica Mouthwash and Miswak Extract. J Contemp Dent Pract 2012;13(2):147-152.
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Raju, Ramyathilagam, Angeline Divya, Ganesh Rajendran, and James Rufus John. "Analogous assay between green tea mouthwash, listerine mouthwash and chlorhexidine mouthwash in plaque reduction, on orthodontic patients: a randomized cross-over study." International Journal Of Community Medicine And Public Health 4, no. 5 (April 24, 2017): 1429. http://dx.doi.org/10.18203/2394-6040.ijcmph20171751.
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Background: The aim of the study was to compare the efficiency of green tea mouthwash, Listerine mouthwash and Chlorhexidine mouthwash in plaque reduction among orthodontic patients. Methods: The study employed a double blinded, simple randomized, cross over design with a control group consisting of 30 orthodontic patients undergoing fixed appliance therapy. All the subjects were divided into group 1 (Green tea), group 2 (Listerine) and group 3 (Chlorhexidine) as 10 subjects per group. Gingival status was assessed using Sulcus Bleeding Index and plaque accumulation was assessed using Turesky-Gilmore-Glickman modification of Quigley Hein Index. After a relapse period of 15 days, group 1 and 2 were crossed over, however, group 3 remained the same. Indices were again recorded at baseline and 15th day. Results: The mean gingival and plaque score was reduced in all the three groups. However, green tea mouthwash was estimated to have the highest mean difference from 2.17 ± 0.610 at baseline to 1.48 ± 0.474 on the 15th day. Conclusions: Effective use of mouthwashes as supplements for tooth brushing has proved to be beneficial in oral hygiene and maintenance. The findings of this study provide useful insights on the effectiveness of different compositions of mouthwashes.
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Khoshbakht, Zoleikha, Ehsan Khashabi, Laleh Khodaie, Mohammad Ali Torbati, Farzaneh Lotfipour, and Hamed Hamishehkar. "Evaluation of Herbal Mouthwashes Containing Zataria Multiflora Boiss, Frankincense and Combination Therapy on Patients with Gingivitis: A Double-Blind, Randomized, Controlled, Clinical Trial." Galen Medical Journal 8 (July 15, 2019): 1366. http://dx.doi.org/10.31661/gmj.v8i0.1366.
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Background: Dental plaques as adhesive microbial aggregates on tooth surfaces are considered the first stage of tooth decay as well as gingivitis. Accordingly, the effect of different antimicrobial mouthwashes on removing dental plaques and preventing their formation has been evaluated in various studies. This study aimed to evaluate the efficacy of herbal mouthwashes containing hydro-alcoholic extract of Zataria multiflora (ZM), Frankincense (FR), and a combination of both (ZM+FR) and compare it with chlorhexidine (CHX) mouthwash in subjects with gingivitis. Materials and Methods: In this randomized, controlled, clinical trial a total of 140 patients with gingivitis were divided into four groups including CHX (control group), ZM, FR, and ZM+FR groups. Plaque index (PI), gingival index (GI), and gingival bleeding index (GBI) were measured in days 1, 14, and 21. Results: All three herbal types of mouthwash significantly improved plaque, gingivitis, and gingival bleeding throughout days 14 to 21 (P<0.001). There was no difference between herbal mouthwash with CHX groups. CHX mouthwash showed the most side effects (54.3%), while ZM mouthwash showed the least side effects and the highest consumer satisfaction (5.7% and 94%, respectively). Conclusion: All of the herbal mouthwashes can be good candidates for controlling gingivitis. Comparing with CHX mouthwash, herbal mouthwashes have lower side effects and negligible alcohol content. Among the herbal mouthwashes, ZM outperforms FR and FR+ZM due to its lower side effects and higher levels of patients’ satisfaction. [GMJ.2019;8:e1366]
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Sadanandan, Sowmya, Suhas S, Sanjay Venugopal, and Kavitha Karur. "Comparative Evaluation of 0.1% Octenidine Mouthwash with 0.2% Chlorhexidine Mouthwash in Prevention of Plaque and Gingivitis – A Clinicomicrobiological Study." RGUHS Journal of Dental Sciences 13, no. 3 (2021): 202–10. http://dx.doi.org/10.26715/rjds.13_3_9.
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Background: Our study aimed to assess the efficacy of 0.1% Octenidine mouthwash as an anti-plaque agent and to assess its effect on gingival inflammation and staining of teeth when compared to 0.2% chlorhexidine gluconate by evaluating the impact on plaque and gingival inflammation as well as on microbial load. Materials and Methods: A three week double blind study was conducted on 69 subjects, aged 20-50 years, with moderate to severe gingivitis. The study population was divided into three groups with 23 subjects in each group: Group A - control subjects received only scaling, Group B received 0.2% chlorhexidine gluconate in addition to scaling and Group C received 0.1% Octenidine mouthwash along with scaling. Clinical and microbiological parameters were recorded at baseline, on 14th day and on 21st day. Subjective and objective criteria were assessed on the 14th day and 21st day. Results: There was a statistically significant reduction in all the tested parameters within all the three groups and between the groups from baseline to 21st day. The highest mean reduction in all the parameters was seen in subjects using 0.1% Octenidine. On comparison with the control group, subjects using the mouthwashes (Group B and Group C) had better improvement in clinical and microbiological parameters from baseline to 21st day. On comparison with chlorhexidine, Octenidine mouthwash significantly reduced plaque (p<0.05) and showed better patient acceptability. However, it was comparable to chlorhexidine in other two tested parameters. Conclusion: Owing to Octenidine’s pronounced and comparable antibacterial properties, it can be a promising candidate for the use in antiseptic mouthwashes.
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Sastrodihardjo, Sumadhi, and Kholidina Imanda Harahap. "DISINFECTION CAPACITY OF MOUTHWASHES USING AS ADMIX SOLUTION OF ALGINATE IMPRESSION." Dentika Dental Journal 19, no. 2 (December 12, 2016): 98–101. http://dx.doi.org/10.32734/dentika.v19i2.408.
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Immersion and spraying with disinfectant solution are the effective disinfection methods for alginate impression. However these methods causes dimensional changes on impression. Therefore mouthwash, which also has disinfectant function on microorganism can be used as mixing solution for alginate impression material. The aim of this study is to analyze antibacterial capacity of mouthwashes that used as admix solution for alginate impression. Samples was made from alginate impression material in tablet form with 15 mm diameter and 1 mm thickness. Total samples are 35 , 5 for admix with aquadest as control, chlorhexidine 0.1%, povidon iodine, sodium fluoride 0.1% wv, respectively. Samples were put on incubated Staphylococcus aureus in petri dish and kept in incubator for 24 hours at 37°C. Immersion was performed by dipping 15 samples of hygedent admix with aquadest into mouthwash liquids for 15 minutes. Inhibition zone was measured by using digital calliper. Statistic analysis was performed by using ANOVA one way and unpaired t-test. The admix with chlorhexidine 0.1% show the inhibition zone by 8.09 mm, povidon iodine 0.52 mm, and sodium fluoride 0.1% wv 2.91 mm. By using immersion method they show 7.63 mm inhibition zone for chlorhexidine 0.1%, 1.51 mm for povidon iodine, and 0.91 mm for sodium fluoride 0.1% wv. There are insignificant differences between admix and immerse with chlorhexidine 0.1% solution (p= 0.25). It can be concluded that chlorhexidine mouthwash 0.1% has the equal antibacterial capacity when used for admix solution nor immerse the alginate impression.
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Sufarnap, Erliera, Kholidina Imanda Harahap, and Terry Terry. "Effect of sodium fluoride in chlorhexidine mouthwashes on force decay and permanent deformation of orthodontic elastomeric chain." Padjadjaran Journal of Dentistry 33, no. 1 (March 31, 2021): 74. http://dx.doi.org/10.24198/pjd.vol33no1.26370.
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Introduction: Orthodontic elastomeric chain is polyurethane elastomer that is widely used among orthodontists due to its functions. Chlorhexidine (CHX) and sodium fluoride (NaF) are listed in mouthwash composition which could affect the mechanical properties of the elastomeric chain. This study was aimed to analyze the effect of sodium fluoride in chlorhexidine mouthwashes on force decay and permanent deformation of orthodontic elastomeric chains. Methods: This research is an experimental analytic laboratory with pretest-posttest control group design. 150 samples of orthodontic chains were divided into three groups. Group 1: artificial saliva (control group); Group 2: 0,1% chlorhexidine gluconate solution (CHX); Group 3: 0,1% chlorhexidine digluconate with sodium fluoride solution (CHX-NaF). The orthodontic elastomeric chain was stretched and maintained at a standardized distance equivalent to a force of 300 g. The measurement of force decay and permanent deformation were performed with digital force gauge and digital caliper (0.01mm) at intervals of the first, seventh, fourteenth, twenty-first, and twenty-eighth days, respectively. Results: The force decay and permanent deformation of the elastomeric chain compared between three groups (control, CHX mouthwash and CHX+NaF mouthwash) showed did not have any significantly different (p-value>0,05) at the first, seventh, fourteenth, twenty-first, and twenty-eighth days, respectively. Conclusion: Sodium fluoride in chlorhexidine mouthwash showing no significant difference among saliva, CHX mouthwash and CHX+NaF mouthwash on force decay and permanent deformation of elastomeric chain.
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Sedigh-Rahimabadi, Massih, Mohammadmehdi Fani, Mahsa Rostami-chijan, Mohammad M. Zarshenas, and Mesbah Shams. "A Traditional Mouthwash (Punica granatum var pleniflora) for Controlling Gingivitis of Diabetic Patients." Journal of Evidence-Based Complementary & Alternative Medicine 22, no. 1 (July 8, 2016): 59–67. http://dx.doi.org/10.1177/2156587216633370.
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This study evaluates the safety and efficacy of Punica granatum var pleniflora mouthwash in treatment of diabetic gingivitis. In a double-blind randomized clinical trial 80 patients with diabetes mellitus and gingivitis were assigned to Golnaar and chlorhexidine 0.2% groups. After using mouthwashes for 2 weeks; participants underwent tooth scaling and the last visit was 2 weeks after scaling. The primary outcome measures were plaque, modified gingival and gingival bleeding indices, and pocket depth. Both interventions had significant improvement on all of the gingival and plaque indices ( P < .001 for all indices). There were no significant differences between Golnaar and chlorhexidine in primary outcome measures except for modified gingival index for which Golnaar mouthwash had a superiority after 2 weeks when comparing with chlorhexidine ( P = .039). Meanwhile, Golnaar mouthwash had no staining effect. Golnaar mouthwash is safe and effective in treatment of gingivitis in diabetic patients although further studies are recommended.
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Bahlouli, Sepideh, Zahra Aghazadeh, Marzieh Aghazadeh, Sevda Shojani, and Hossein Samadi Kafil. "Determining the Antibacterial Activity of Chlorhexidine Mouthwashes with and without Alcohol against Common Oral Pathogens." Journal of Advanced Oral Research 9, no. 1-2 (May 2018): 15–19. http://dx.doi.org/10.1177/2229411218762045.
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Aims and Objectives Mouthwashes with antibacterial activity inhibit the growth of bacteria in the mouth and teeth. Chlorhexidine is one of the most widely used mouthwashes that inhibits dental plaque and prevents tooth surface decay. Recently, concerns have been raised that alcohol-containing mouthwashes may have carcinogenic properties and may be harmful to children and pregnant and lactating women. The aim of this study was to determine the antibacterial effects of chlorhexidine mouthwashes with and without alcohol on common oral bacteria. Material and Methods In this in vitro study, bacterial species were purchased from a research center and were cultured separately in proprietary environments in test tubes. Thereafter, mouthwashes with alcohol, without alcohol, and with salt water (saline) were added to test tubes containing the bacteria grown. The samples were then analyzed using a spectrophotometer to determine viability, growth rate, and bacteria waste. Finally, the data were analyzed using SPSS version 17 through analysis of variance (ANOVA) and Tukey statistical tests. Results The obtained results showed that the saline group had the highest antibacterial activity and that the average antibacterial activity of the alcohol and alcohol-free groups did not differ significantly (P > 0.05). Post hoc test results showed that the antibacterial activity of the saline group was significantly different statistically from that of the other two groups. Conclusion On the basis of the results, it can be concluded that both alcohol-free chlorhexidine and alcohol-containing chlorhexidine are effective in removing oral microbes. Moreover, by using alcohol-free chlorhexidine, the harmful effects of alcohol can be prevented.
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Jiang, Qingru, Veera Kainulainen, Iva Stamatova, Sok-Ja Janket, Jukka H. Meurman, and Riitta Korpela. "Mouthwash Effects on LGG-Integrated Experimental Oral Biofilms." Dentistry Journal 8, no. 3 (September 1, 2020): 96. http://dx.doi.org/10.3390/dj8030096.
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In order to investigate the effects of mouthwashes on oral biofilms with probiotics, we compared in biofilms the susceptibility to mouthwashes of probiotic Lactobacillus rhamnosus GG (LGG) and oral pathogens Streptococcus mutans, Streptococcus sanguinis, and Candida albicans. We also evaluated these pathogens’ susceptibility to the mouthwashes and their recovery after mouthwash-rinsing in biofilms with/without LGG. First, 1-day-/3-day-old LGG-integrated multi-species biofilms were exposed for 1 min to mouthwashes containing chlorhexidine, essential oils, or amine fluoride/stannous fluoride. Cells were plate-counted and relative survival rates (RSRs) of LGG and pathogens calculated. Second, 1-day-/3-day-old multispecies biofilms with and without LGG were exposed for 1 min to mouthwashes; cells were plate-counted and the pathogens’ RSRs were calculated. Third, 1-day-old biofilms were treated for 1 min with mouthwashes. Cells were plate-counted immediately and after 2-day cultivation. Recovery rates of pathogens were calculated and compared between biofilms with/without LGG. Live/Dead® staining served for structural analyses. Our results showed that RSRs of LGG were insignificantly smaller than those of pathogens in both 1-day and 3-day biofilms. No significant differences appeared in pathogens’ RSRs and recovery rates after treatment between biofilms with/without LGG. To conclude, biofilm LGG was susceptible to the mouthwashes; but biofilm LGG altered neither the mouthwash effects on oral pathogens nor affected their recovery.
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Champaneri, Harshal Rajeshbhai, Nirma Yadav, Barkha Makhijani, Neema Shetty, Aditi Mathur, and Balaji Manohar. "Effectiveness of Indigenously Prepared Punica Granatum and Camellia Sinesis Mouthwashes as an adjunct to Non Surgical Periodontal Therapy: A Clinical Trial." Journal of Nepalese Society of Periodontology and Oral Implantology 1, no. 1 (June 1, 2017): 27–31. http://dx.doi.org/10.3126/jnspoi.v1i1.23524.
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Background: Chronic periodontitis is a bacterially induced chronic disease that is immune-inflammatory in nature. The incidence and progression of this disease is related to a substantial increase in gram negative anaerobic rods. The use of antimicrobial mouthwashes as an adjunct to mechanical plaque control is well established in dental practice. Punica granatum and Camellia sinesis extract are naturally occurring antimicrobial agents. The active ingredient in Punica granatum and Camellia sinesis is polyphenolic flavonoid, which is an antioxidant in nature. It inhibits the production of arachidonic acid metabolites such as pro- inflammatory prostaglandins and leukotriens, resulting in a decreased inflammatory response.
Aim: To evaluate the efficacy of indigenously prepared Punica granatum and Camellia sinesis mouthwashes to the commercially available chlorhexidine mouthwash.
Materials and Methods: Thirty subjects diagnosed with chronic generalized marginal gingivitis were selected and randomly divided into three groups. Group 1 – Camellia sinesis mouthwash group, Group 2 – Punica granatum mouthwash group and Group 3 – Chlorhexidine mouthwash group. Subjects were instructed to use the prescribed mouthwash for 14 days. Plaque index, gingival index and calculus component of periodontal index were recorded at baseline and at 14 days.
Results: The clinical study observed significant improvement in plaque and gingival status at all sites (p<0.05). Camellia sinesis and Punica granatum mouthwash showed significant improvement in plaque, gingival and calculus score.
Conclusion: Camellia sinesis and Punica granatum mouthwash are beneficial in improving gingival status due to its profound styptic action, with sufficient reduction in plaque scores.
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Song, In-Seok, Ji Eun Lee, and Jun-Beom Park. "The effects of various mouthwashes on osteoblast precursor cells." Open Life Sciences 14, no. 1 (July 22, 2019): 376–83. http://dx.doi.org/10.1515/biol-2019-0042.
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AbstractThis study examined whether or not various mouthwashes have significant effects on the viability or morphology of mouse osteoblast-like cells. Mouse calvarial preosteoblast cells were cultured and prepared, then treated with a 0.12% chlorhexidine digluconate solution containing essential oils with or without alcohol, and a cetylpyridinium chloride solution, and sodium fluoride, respectively. Each well was treated with one of six mouthwashes for either 30 sec, 1.5 min, or 4.5 min. The viability of the treated cells was quantitatively analyzed by a Cell Counting Kit-8. The viability of osteogenic progenitor cells decreased significantly irrespectively of the types of mouthwashes. The changes of cell morphology were seen in all groups of mouthwashes; however, they were more noticeable on the chlorhexidine digluconate-treated group. A progressive increase in treatment time over 30 sec did not seem to deteriorate cellular viability. There was no significant difference in viability or morphological change between different formulations of the same brand. Although various mouthwashes without alcohol as an ingredient are available, nonalcoholic mouthwashes were not likely to be less harmful to the cells. Collectively, commercially available mouthwashes could inhibit cell viability and alter the morphology of osteoblastic precursor cells irrespectively of brands, treatment time, or alcohol content.
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Tonetto, Mateus Rodrigues, Matheus Coelho Bandéca, Etevaldo Matos Maia Filho, José Carlos Elias Mouchrek Junior, Lívia Helena de Araújo Castro Nunes, Cleidiane Silveira Arruda, Claudia de Castro Rizzi, Adriana Quinzeiro e. Silva Mouchrek, and Rudys Rodolfo De Jesus Tavarez. "Effectiveness of Oral Antiseptics on Tooth Biofilm: A Study in vivo." Journal of Contemporary Dental Practice 16, no. 8 (2015): 674–78. http://dx.doi.org/10.5005/jp-journals-10024-1739.
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ABSTRACT Aim To evaluate the effectiveness of five different mouthwashes through measurement of the plaque index. Materials and methods Fifty subjects took part in this blind study, randomized into blocks of five groups according to the active ingredient of the mouthwash: CHX group (0.12% chlorhexidine gluconate), essential oils (EO) group, cetylpyridinium chloride (CPC) group, Tri group (triclosan) and Hamamelis virginiana (HV) group. All subjects were evaluated for a reduction in the bacterial plaque index at 7, 14 and 21 days. Results There was a significant reduction in the mean plaque index during the period of evaluation (p < 0.01), and the reduction during the period of evaluation was different between mouthwashes (p < 0.01). The reduction in the plaque index at the end of 21 days was, in decreasing order, CHX > EO > CPC > Tri > HV. Conclusion The reduction in the plaque index during the period of evaluation was different between the types of mouthwash. The mouthwash containing the active ingredient chlorhexidine was the most effective, followed by the essential oil, cetylpyridinium chloride, triclosan and H. virginiana. How to cite this article Junior Mouchrek JCE, Nunes LHAC, Arruda CS, Rizzi CC, Mouchrek AQS, Tavarez RRJ, Tonetto MR, Bandeca MC, Maia Filho EM. Effectiveness of Oral Antiseptics on Tooth Biofilm: A Study in vivo. J Contemp Dent Pract 2015;16(8):674-678.
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Jamali, Zahra, Mahdieh Alipour, Syamand Ebrahimi, and Marzie Aghazadeh. "Effect of Halita mouthwash on oral halitosis treatment: A randomized triple-blind clinical trial." Journal of Dental Research, Dental Clinics, Dental Prospects 13, no. 1 (April 24, 2019): 31–35. http://dx.doi.org/10.15171/joddd.2019.005.
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Background. Halitosis (oral malodor) is a common problem all over the world and its prevalence has been estimated at 23‒ 50%. Halitosis originates from oral cavity in 85% of patients. This clinical trial was conducted to evaluate the efficacy of the Halita mouthwash in oral halitosis treatment. Methods. Fifty subjects with an organoleptic score of >2 at baseline participated in this triple-blinded clinical trial. Subjects were divided into 2 groups. Group I subjects (N=25) were instructed to rinse with 0.2% chlorhexidine (CHX) mouthwash twice a day for 1 week. Group II subjects (N=25) used Halita mouthwash with the same instruction. Halitosis was evaluated at baseline and one week after using the mouthwashes by organoleptic method. Data were analyzed with chi-squared and Mann-Whitney U tests (P<0.05). Results. In the Halita group subjects exhibited 2.04±0.65 reduction in OLS. OLS reduction in the chlorhexidine group was 1.95±0.74. Statistical analysis showed no significant difference between the two groups (P>0.05). Conclusion. Based on the results, Halita mouthwash has the same effect on oral halitosis as routine 0.2% CHX mouthwash. Halita mouthwash has fewer side effects because of lower concentration of chlorhexidine. Therefore 0.2% CHX mouthwash could be replaced by Halita mouthwash for the treatment of halitosis.
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Erriu, Matteo, Francesca Maria Giovanna Pili, Enrica Tuveri, Daniela Pigliacampo, Alessandra Scano, Caterina Montaldo, Vincenzo Piras, et al. "Oil Essential Mouthwashes Antibacterial Activity againstAggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects." International Journal of Dentistry 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/164267.
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The aim of this work is to determine the antibacterial activity of three marketed mouthwashes on suspended and sessile states ofAggregatibacter actinomycetemcomitans. The efficacy of two commonly used products in clinical practice, containing essential oils as active ingredients (menthol, thymol, methyl salicylate, and eucalyptol) in association with or without alcohol, has been evaluated in comparison with a chlorhexidine-based mouthwash. The microtiter plate assay, in order to obtain a spectrophotometric measurement of bacterial responses at growing dilutions of each antiseptic, was used for the study. The analysis revealed that a good antibacterial activity is reached when the abovementioned mouthwashes were used at concentration over a 1/24 dilution and after an exposure time of 30 seconds at least. In conclusion, the alcoholic mouthwash appears to have a better biofilm inhibition than its antiplanktonic activity while the nonalcoholic product demonstrates an opposite effect with a better antiplanktonic behavior.
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Buakaew, Watunyoo, Rungnapa Pankla Sranujit, Chanai Noysang, Supaporn Sangouam, Nungruthai Suphrom, Yordhathai Thongsri, Pachuen Potup, and Kanchana Usuwanthim. "Evaluation of Mouthwash Containing Citrus hystrix DC., Moringa oleifera Lam. and Azadirachta indica A. Juss. Leaf Extracts on Dental Plaque and Gingivitis." Plants 10, no. 6 (June 6, 2021): 1153. http://dx.doi.org/10.3390/plants10061153.
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Oral hygiene and control of microbial plaque biofilm formation are effective methods for preventing gingivitis. Mouthwashes containing leaf extracts of the medicinal plants Citrus hystrix DC. (KL), Moringa oleifera Lam. (MO) and Azadirachta indica A. Juss. (NE) were assessed for oral healthcare and gingivitis adjunctive treatment. Three types of mouthwash were developed; KL, a combination of KL and MO (KL + MO), and a combination of KL, and NE (KL + NE). The mouthwashes were tested in vivo on 47 subjects with gingivitis who were allocated into five groups as (i) placebo, (ii) KL, (iii) KL + MO, (iv) KL + NE, and (v) 0.12% chlorhexidine gluconate (CHX). Participants were instructed to rinse with herbal mouthwash twice daily for two weeks. Gingival index (GI), plaque index (PI), and oral microbial colonies were measured at baseline and 15 days. Results showed that GI and PI of groups (ii)–(iv) significantly decreased over the placebo group, while accumulative reduction percentages of both Staphylococcus spp. and Candida spp. were found in groups (iii) and (iv). Findings indicated that the herbal mouthwashes reduced GI and PI, and showed potential as oral healthcare products.
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Goel, Deepak, Aayushi Bansal, and Anant Gopal Nigam. "Effect of Achyranthes aspera, 0.2% Aqueous Chlorhexidine Gluconate and Punica granatum Oral Rinse on the Levels of Salivary Streptococcus mutans in 8 to 12 Years Old Children." Journal of Contemporary Dental Practice 16, no. 11 (2015): 903–9. http://dx.doi.org/10.5005/jp-journals-10024-1779.
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ABSTRACT Background and objectives To study the effect Achyranthes aspera, 0.2% aqueous chlorhexidine gluconate and Punica granatum oral rinse on salivary Streptococcus mutans count in children. Materials and methods A total of 60 children of 8 to 12 years of age were randomly allocated into 3 groups. Group A was given 0.2% chlorhexidine mouthwash, group B was given 10% A. aspera mouthwash and group C was given 15% P. granatum mouthwash. The day 1 saliva samples were collected from the subjects and innoculated onto mitis salivarius bacitracin (MSB) agar. The colony counts were obtained by a clinical microbiologist who was blinded to the subject allocation. Plaque scores were then recorded by the investigator with the help of a volunteer. Following this, they received a thorough scaling and polishing. Subjects in each group were then provided with 140 ml of the respective mouthwash, as a daily supervised rinse after breakfast and before sleeping as per instructions. Following mouth rinsing, the children were instructed not to eat or drink for 15 minutes. At the 7th day, unstimulated saliva was again collected from the subjects of all 3 groups, inoculated onto MSB agar and colony count was obtained. Modified Quigley- Hein plaque index was also evaluated for the refreshed score at this stage. Colony counting was done using loop method and statistical analysis was done using Statistical Package for the Social Science (SPSS) software version 21. Results All the three mouthwashes showed statistically significant reduction of S. mutans count and plaque index after 7 days, i.e. chlorhexidine (p < 0.001 for reduction in S. mutans count and p < 0.05 for plaque score reduction), A. aspera (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction) and P. granatum (p < 0.01 for reduction in S. mutans count and p < 0.05 for plaque score reduction). Chlorhexidine had marginally better results in reducing S. mutans count. Conclusion • Efficacy of chlorhexidine, A. aspera and P. granatum was statistically significant with respect to reduction of S. mutans count with chlorhexidine being marginally better than the other two, • All the three mouthwashes were found to be at par when plaque index values from baseline and after interception of 7 days was calculated, • Punica granatum has better antimicrobial effect than A. aspera. How to cite this article Bansal A, Marwah N, Nigam AG, Goenka P, Goel D. Effect of Achyranthes aspera, 0.2% Aqueous Chlorhexidine Gluconate and Punica granatum Oral Rinse on the Levels of Salivary Streptococcus mutans in 8 to 12 Years Old Children. J Contemp Dent Pract 2015;16(11):903-909.
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Ali El Basuony, Shaimaa Ali Hamouda, Naglaa El Hossary, and Nermine Raouf Amin. "Apoptosis inducing effects of chlorhexidine and essential oil mouthwashes on BHK-21 fibroblast cell line: An in vitro study." F1000Research 7 (October 26, 2018): 1703. http://dx.doi.org/10.12688/f1000research.16337.1.
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Background: The maintenance of oral health can be achieved mainly by mechanical and chemical means. Among chemical agents, mouthwashes are widely used for personal oral hygiene because of their ability to inhibit dental plaque. The antibacterial effects of essential oils (EOs) and chlorhexidine (CHX) are well documented; however, the reaction of host tissue to these substances has a poor documentation. Until now studies have not examined the effect of EOs with sodium fluoride (EOF) on fibroblast cell lines. The aim of this study was to examine the effect of mouth rinse EOs, EOF and CHX on the apoptosis of fibroblast cell line. Methods: BHK-21 fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium containing EOs, EOF and CHX mouthwashes with different doses (15% or 25%) and various exposure times. Cell apoptosis was assayed using RT-PCR. Results: EOs, EOF and CHX induce apoptotic effects on fibroblasts in a dose and time dependent manner. Conclusion: CHX is the most cytotoxic mouthwash to fibroblasts as compared to mouthwashes containing EOs and EOF.
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Thakur, Srinath, Shruti Malagi, and Anirudh B. Acharya. "Evaluation of the Antimicrobial and Anti-inflammatory Efficacy of Two Commercially Available Mouthwashes." Journal of Contemporary Dentistry 7, no. 2 (2017): 119–21. http://dx.doi.org/10.5005/jp-journals-10031-1197.
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ABSTRACT Introduction Various chemical agents are being used as an adjunct to mechanical therapy. Chlorhexidine (CHX), though considered as the gold standard, has certain side effects. The use of herbal products as an adjunctive therapy is thus gaining more popularity. The aim of this study was to evaluate the antimicrobial and anti-inflammatory efficacies of two commercially available mouthwashes. Materials and methods Antimicrobial activity of the mouthwashes was analyzed in vitro by evaluating their minimal inhibitory concentration (MIC) against four microorganisms. The anti-inflammatory efficacy was evaluated in 84 individuals, who were divided into four groups of 21 each based on their gingival index (GI) score. The subjects used the respective mouthwashes allotted to them for 2 weeks. Plaque index (PI), GI, and modified sulcus bleeding index (mSBI) of the participants were recorded at baseline and 14 days. Results The herbal mouthwash was effective against the tested organisms in vitro. The PI and GI scores reduced in all the four groups at the end of 14 days with the CHX group showing more reduction. The herbal mouthwash showed significantly better clinical outcomes when compared with other groups and results comparable to CHX. Conclusion The herbal mouthwash showed antimicrobial and anti-inflammatory effects comparable with that of 0.2% CHX, and may have a potential as an adjunct to scaling and root planing. How to cite this article Thakur S, Malagi S, Acharya AB. Evaluation of the Antimicrobial and Anti-inflammatory Efficacy of Two Commercially Available Mouthwashes. J Contemp Dent 2017;7(2):118-121.
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Jafari, Karim, Saeed Rahimzadeh, and Somayeh Hekmatfar. "Nickel ion release from dental alloys in two different mouthwashes." Journal of Dental Research, Dental Clinics, Dental Prospects 13, no. 1 (April 24, 2019): 19–23. http://dx.doi.org/10.15171/joddd.2019.003.
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Background. Mouthwashes are widely used as adjuncts to mechanical oral hygiene procedures. Nonetheless, there is little information regarding the effect of various mouthwashes on the amount of ions released from the nickel-chromium (Ni‒Cr) alloys used in the fabrication of fixed prostheses. Therefore, the present study was conducted to evaluate the effect of two types of mouthwash on the release of Ni ions from dental alloys. Methods. Forty-two disk-shaped specimens were prepared with a diameter of 10 mm and a height of 2 mm. Two mouthwashes were examined in this study: Oral B and Listerine. A control group was also considered using distilled water. Each Ni‒Cr disk was immersed in the mouthwashes and distilled water in polypropylene test tubes, and then incubated at 37°C to simulate the oral temperature. After 45 days of incubation, the samples were tested for Ni ions using inductively coupled plasma mass spectroscopy. Data were analyzed using ANOVA. Results. In the Halita group subjects exhibited 2.04±0.65 reduction in OLS. OLS reduction in the chlorhexidine group was 1.95±0.74. Statistical analysis showed no significant difference between the two groups (P>0.05). Conclusion. As the results indicated, the amount of ion release was within the safe limits in the two experimental groups. However, it is recommended that prescribe Listerine mouthwash should not be prescribed for the patients with a history of Ni allergy.
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Zarandi, Ali, Sina Salahaddin, and Masoumeh Faramarzi. "Efficacy of Different Concentrations of Chlorhexidine Mouthwash on Plaque Accumulation and Periodontal Parameters." Journal of Periodontology & Implant Dentistry 8, no. 1 (October 8, 2016): 8–11. http://dx.doi.org/10.15171/jpid.2016.002.
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Background and aims. Dental plaque and gingivitis were controlled by administration of chemical agents such as chlorhexidine (CHX) which is recognized as a gold standard of chemical agents. The aim of this study was to compare the effects of two mouthwashes (0.2% CHX and Kin Gingival) on clinical parameters. Materials and methods. A total of 88 subjects were included in this interventional‒experimental study. The subjects were divided into two groups of 44 (group 1: 0.2% CHX and group 2: Kin Gingival). The study involved no mechanical plaque control methods. Patients used the mouthwashes twice a day for two weeks. Clinical parameters included plaque index (PI), gingival index (GI), probing depth (PD) and bleeding on probing (BOP), which were measured before and after the use of mouthwashes. The results were analyzed by Man-Whitney U and chi-squared tests. Statistical significance was set at P < 0.05. Results. The results indicated that PI, GI and PD significantly decreased in group 2 (Kin Gingival) in comparison with group 1 (0.2% CHX) (P < 0.05). However, the two mouthwashes did not differ significantly from each other in relation to BOP (P > 0.05). Conclusion. Based on the results it can be concluded that Kin Gingival and 0.2% CHX mouthwashes decrease the clinical parameters in patients significantly. However, Kin Gingival is more effective than 0.2% CHX, which might be attributed to the synergic antibacterial potential of Kin Gingival ingredients like sodium fluoride.
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Nishad, A., NS Sreesan, Joseph Joy, Lakshmi Lakshmanan, Joyce Thomas, and VA Anjali. "Impact of Mouthwashes on Antibacterial Activity of Subjects with Fixed Orthodontic Appliances: A Randomized Clinical Trial." Journal of Contemporary Dental Practice 18, no. 12 (2017): 1112–16. http://dx.doi.org/10.5005/jp-journals-10024-2185.
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ABSTRACT Aim The study aimed to assess the impact of mouthwashes on antibacterial activity of individuals with fixed orthodontic appliances. Materials and methods A total of 60 individuals were considered in the study. Sixty (20 each group) nonextraction class I individuals were randomly divided into experimental and control groups. Group I: Experimental group [chlorhexidine (CHX) mouthwash], group II: Experimental group (neem mouthwash), group III: Control group (distilled water). All the clinical examinations were done at baseline and 30th day respectively, after the start of orthodontic treatment. The mean differences between the different experimental groups were calculated using one-way analysis of variance (ANOVA) test. Results There was no statistical significance at baseline mean plaque index (PI), gingival index (GI) scores, and Streptococcus mutans (SM) colony count between groups. The PI and GI scores among CHX and neem mouthwash groups (p = 0.002, p = 0.032 respectively) were significantly reduced after intervention and also the SM colonies count was reduced in CHX and neem mouthwash groups and there was significant difference between the groups. Conclusion As both mouthwashes showed significant effectiveness on antibacterial activity in individuals with fixed orthodontic appliances, neem mouthwash can be used as an alternative to CHX. Clinical significance It is better to have a sound knowledge regarding the use of mouthwash in long term as fixed orthodontics are associated with accumulation of SM, enamel demineralization, and an increased number of carious lesions, predominantly in sites adjacent to bracket. How to cite this article Nishad A, Sreesan NS, Joy J, Lakshmanan L, Thomas J, Anjali VA. Impact of Mouthwashes on Antibacterial Activity of Subjects with Fixed Orthodontic Appliances: A Randomized Clinical Trial. J Contemp Dent Pract 2017;18(12):1112-1116.
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Kasuma, Nila, Fildzah Nurul Fajrin, and Yufri Aldi. "MORINDA CITRIFOLIA EXTRACT MOUTHWASH AS ANTIGINGIVITIS." Dentika Dental Journal 19, no. 2 (December 12, 2016): 102–9. http://dx.doi.org/10.32734/dentika.v19i2.409.
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Gingivitis is a inflamatory mild form of periodontal disease in gingiva. The early stage of gingivitis characterized by the accumulation of plaque, leukocytes, and PMN. Noni (Morinda citrifolia L.) is known to have anti-inflammatory compositions which also affects the activity of leukocytes during inflammation. The research objective was to analyze the clinical effects of mouthwash that contains extracts of noni (Morinda citrifolia L.) on leukocyte levels in the saliva in patients with gingivitis, in order to obtain traditional mouthwash that is economical, readily available, and effective in reducing gingivitis and can be used by the public. Extracts of noni fruit (Morinda citrifolia L.) is given in the form of mouthwashes used for 7 days in a row. Observations conducted on three groups of respondents: group 1 using mouthwash placebo, group 2 using mouthwash noni fruit extract 5%, and group 3 using chlorhexidine 0.1%. The use of mouthwash done in the morning and at night before bed as much as 15 ml for 30 seconds. The results showed a decrease in the number of leukocytes in the group treated with placebo cyst, 5% noni fruit extract, and chlorhexidine 0.1% (p <0.05). Both test preparations equally decreased the number of leukocytes in gingival sulcus in gingivitis patients.
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Becker, Kathrin, Giulia Brunello, Luisa Scotti, Dieter Drescher, and Gordon John. "Efficacy of 0.05% Chlorhexidine and 0.05% Cetylpyridinium Chloride Mouthwash to Eliminate Living Bacteria on In Situ Collected Biofilms: An In Vitro Study." Antibiotics 10, no. 6 (June 17, 2021): 730. http://dx.doi.org/10.3390/antibiotics10060730.
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Chlorhexidine (CHX) mouthwashes are frequently used as an adjunctive measure for the treatment of periodontitis and peri-implantitis, as well as in patients on maintenance therapy. However, their prolonged use is associated with several side effects. This study aimed at evaluating if a mouthwash with a reduced concentration of CHX combined with cetylpyridnium chloride (CPC) was as effective as a conventional CHX mouthwash in the reduction in living cells in oral biofilms attached to hydroxyapatite (HA) and micro-rough titanium (Ti) surfaces. Four healthy volunteers wore a customized acrylic appliance containing HA and Ti discs for in situ plaque accumulation. Biofilms were grown on the discs for 24 or 48 h and then randomly exposed for 60 s to: 0.05% CHX + 0.05% CPC, 0.1% CHX (positive control) or sterile saline (negative control). Viability assay and live-dead staining were performed to quantify bacterial viability and to distinguish live and dead cells, respectively. At both time points, contrary to saline, CHX, both alone and in combination with CPC, exhibited high antibacterial properties and induced a significant reduction in biofilm viability. This study demonstrates the potential of mouthwashes containing a low concentration of CHX combined with CPC as effective antibacterial agents for long-term applications with reduced undesired side effects.
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Öznurhan, Fatih, Burak Buldur, Özgül Cartı, Uğur Tutar, Cem Çelik, and Ceylan Hepokur. "Antimicrobial Efficacy of Chlorhexidine and Licorice Mouthwashes in Children." Meandros Medical and Dental Journal 20, no. 1 (April 1, 2019): 13–19. http://dx.doi.org/10.4274/meandros.galenos.2018.79663.
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Oyanagi, Takehiro, Junji Tagami, and Khairul Matin. "Potentials of Mouthwashes in Disinfecting Cariogenic Bacteria and Biofilms Leading to Inhibition of Caries." Open Dentistry Journal 6, no. 1 (January 19, 2012): 23–30. http://dx.doi.org/10.2174/1874210601206010023.
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Objectives:The aim of this study was to compare the effects of certain commercially available mouthwashes on cariogenic bacteria and biofilms, following the acquisition of inhibition potentials of caries.Materials and Methods:Mouthwashes containing I) chlorhexidine gluconate (CHG; 0.0005% w/v), II) benzethonium chloride (BTC; 0.01% w/v), III) an essential oil (Listerine), and IV) povidone-iodine (PVP-I; 0.035% w/v) were tested on planktonic cariogenic bacteria, biofilms, and an ex vivo caries model. Bacterial aliquots were inoculated with each solution separately and vortexed for 10 seconds at room temperature. Bacterial viability was subsequently investigated by fluorescence microscopy (FM) after staining with a BacLight viability kit and the number of colony-forming units (CFUs) was counted. Similarly, mouthwash solutions were applied to artificial cariogenic biofilms, and bacterial viability of the biofilms was investigated as stated above. Inhibition potentials of two selected mouthwashes of carious lesions were investigated using biofilm-induced caries and a secondary caries model. In all steps, a phosphate-buffered saline (PBS) solution was included as a control.Results:Planktonic cariogenic bacteria and bacteria embedded in biofilms were killed in remarkably large numbers with Listerine and PVP-I treatment compared to PBS and other gargles. CFU counts also showed significant reduction after treatment with Listerine and PVP-I compared to other solutions (P<0.05). Listerine also displayed significant (P<0.05) inhibition effects in preventing the progression of demineralization.Conclusion:Bactericidal potencies of the mouthwashes varied significantly, suggesting that mouthwashes like Listerine can be useful for the prevention of caries and secondary caries.
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Cai, He, Junyu Chen, Nirmala K. Panagodage Perera, and Xing Liang. "Effects of Herbal Mouthwashes on Plaque and Inflammation Control for Patients with Gingivitis: A Systematic Review and Meta-Analysis of Randomised Controlled Trials." Evidence-Based Complementary and Alternative Medicine 2020 (January 20, 2020): 1–16. http://dx.doi.org/10.1155/2020/2829854.
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Objective. The aim of this study was to evaluate the overall effects of herbal mouthwashes as supplements to daily oral hygiene on plaque and inflammation control compared with placebos and chlorhexidine (CHX) mouthwashes in the treatment of gingivitis. Methods. PubMed, EMBASE, Cochrane Database of Systematic Reviews, Cochrane Central Register of Controlled Trials, and grey literature databases were searched. Only randomised controlled trials (RCTs) comparing herbal mouthwashes with placebos or CHX in the daily oral hygiene of patient with gingivitis were included to compare the effect of different mouthwashes on plaque and inflammation control. Results. A total of 13 studies satisfied the eligibility criteria, and 11 studies were included in meta-analyses. Significant differences were observed in favour of herbal mouthwashes compared with placebos in both plaque- and inflammation-related indices (Quigley-Hein Plaque Index, QHPI: WMD = −0.61, 95% CI (−0.80, −0.42), P<0.001; Gingival Index, GI: −0.28 (−0.51, −0.06), P=0.01; Modified Gingival Index, MGI: −0.59 (−1.08, −0.11), P=0.02; Gingival Bleeding Index, GBI: −0.06 (−0.09, −0.04), P<0.001). No significant difference was found between herbal and CHX mouthwashes. Conclusions. Herbal mouthwashes have potential benefits in plaque and inflammation control as supplements to the daily oral hygiene of patients with gingivitis. Although no difference was observed between herbal and CHX mouthwashes in the selected studies, further high-quality RCTs are needed for more firm support before advising patients with gingivitis about whether they can use herbal mouthwashes to substitute for CHX mouthwashes or not (PROSPERO registration number: CRD42019122841).
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Masocatto, Danilo Chizzolini, Mariza Akemi Matsumoto, Tulio Marcos Kalife Coelho, Ellen Cristina Gaetti Jardim, José Carlos Garcia de Mendonça, Julio Cesar Leite da Silva, Carlos Alexandre Carollo, et al. "Comparison of antimicrobial activity of thymol and carvacrol to chlorhexidine in surgery." Research, Society and Development 10, no. 7 (June 13, 2021): e4310716310. http://dx.doi.org/10.33448/rsd-v10i7.16310.
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Currently, there is a constant search for new chemical compounds through plant extracts and phytochemical compounds that do not cause so many side effects in the oral cavity and that have good antimicrobial properties. Therefore, the present study evaluated the synergistic antimicrobial efficacy of mouthwashes containing thymol and carvacrol when compared with 0.12% chlorhexidine mouthwash after bilateral lower third molar extraction. Nineteen healthy (male and female) patients (n = 19) aged between 18 and 30 years, of both sexes, treated at the Faculty of Dentistry of Mato Grosso do Sul (UFMS, MS, Brazil) participated in this study. All patients were submitted to two different therapeutic treatments, divided into two groups: group 1 (control) - patient used 0.12% chlorhexidine mouthwash solution; group 2 (experimental) - patient used 0.05% thymol and 0.025% carvacrol solutions. The suture was removed at seven postoperative days and aseptically transferred to a tube containing sterile potassium buffer saline solution. Samples were submitted to microbiological analysis at the Microbiology Laboratory at UFMS. The mean and standard deviation of colony forming units/ml of isolate microorganisms in the suture grown in BHI agar obtained from the control and experimental groups were 4.766 x 106 ± 4.069 and 4.847 x 106 ± 3.971, respectively. In conclusion, the thymol and carvacrol based mouthwash showed antimicrobial effectivity in biofilm adherence of the silk thread after extraction of third molars.
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Javali, Mukhatar Ahmed, Mohasin Abdul Khader, Razan Mansour Alqahtani, Muna Jubran Almufarrij, Thamra Mohammed Alqahtani, and Mohamed Khaled Addas. "Spectrophotometric Analysis of Dental Enamel Staining to Antiseptic and Dietary Agents: In Vitro Study." International Journal of Dentistry 2020 (June 5, 2020): 1–5. http://dx.doi.org/10.1155/2020/5429725.
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Background/Objectives. Use of antiseptics as an adjunct to a traditional mechanical tooth brushing method has limited their application for long duration because of their side effects such as staining and calculus formation. The objective of this in vitro study is to analyse the staining effects of antiseptic mouthwashes on dental enamel and compare it with those containing nanoparticles, dietary agents, and distilled water (control). Material and Methods. 105 intact premolars extracted for orthodontic reasons and without any caries or anatomical defects were selected for analysis. The samples were randomly divided into 7 different groups of fifteen teeth each for different solutions. A spectrophotometer was used to assess the colorimeter analysis of buccal dental enamel surface at R1 (baseline examination), R2 (24 hours after immersion in different solutions), and R3 (after brushing). Statistical analysis was done using the Kolmogorov–Smirnov test and Levene’s test (p<0.05), respectively. One-way ANOVA was used to compare the difference in color (∆E) between the readings, R1, R2, and R3. Results. The mouthwash containing titanium dioxide (TiO2) nanoparticles produced the greater enamel discoloration compared to that of chlorhexidine. Brushing had little effect on removal of stains induced by all mouthwashes except for dietary solutions (lemon with sodium bicarbonate and olive with laurel) and distilled water (control). Conclusion. The results from this study show that mouthwashes containing TiO2 nanoparticles and other antiseptic mouthwashes cause change in color of the teeth and lead to poor esthetic appearance when compared to dietary and control solutions. Thus, future in vivo studies have to be conducted to confirm these findings as in vitro studies may not provide a reliable simulation of the clinical situations.
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Anderson, Gissela Bernal, Jim Bowden, Edith C. Morrison, and Raul G. Caffesse. "Clinical effects of chlorhexidine mouthwashes on patients undergoing orthodontic treatment." American Journal of Orthodontics and Dentofacial Orthopedics 111, no. 6 (June 1997): 606–12. http://dx.doi.org/10.1016/s0889-5406(97)70312-3.
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Rajendiran, Meenakshi, Harsh M. Trivedi, Dandan Chen, Praveen Gajendrareddy, and Lin Chen. "Recent Development of Active Ingredients in Mouthwashes and Toothpastes for Periodontal Diseases." Molecules 26, no. 7 (April 1, 2021): 2001. http://dx.doi.org/10.3390/molecules26072001.
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Periodontal diseases like gingivitis and periodontitis are primarily caused by dental plaque. Several antiplaque and anti-microbial agents have been successfully incorporated into toothpastes and mouthwashes to control plaque biofilms and to prevent and treat gingivitis and periodontitis. The aim of this article was to review recent developments in the antiplaque, anti-gingivitis, and anti-periodontitis properties of some common compounds in toothpastes and mouthwashes by evaluating basic and clinical studies, especially the ones published in the past five years. The common active ingredients in toothpastes and mouthwashes included in this review are chlorhexidine, cetylpyridinium chloride, sodium fluoride, stannous fluoride, stannous chloride, zinc oxide, zinc chloride, and two herbs—licorice and curcumin. We believe this comprehensive review will provide useful up-to-date information for dental care professionals and the general public regarding the major oral care products on the market that are in daily use.
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Sam, Jonathan E., Paulaian Benin, Ruth H. Beaulah, Gnanaseelan LNU, Lal Krishna, and Jacob Raja. "Comparative Evaluation of Antibacterial Efficacy of Four Toothpastes and Mouthwashes against Streptococcus mutans and Lactobacillus: An in vivo Study." Journal of Operative Dentistry & Endodontics 1, no. 2 (2016): 60–65. http://dx.doi.org/10.5005/jp-journals-10047-0013.
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ABSTRACT Background Cariogenic microorganisms are the most important cause for occurrence of dental caries. Dentifrices and mouthwashes containing antimicrobial substances are proven to be effective in the eradication of these pathogens from the oral cavity. Aim To evaluate the antimicrobial efficacy of fluoride, chlorhexidine (CHX), herbal, and xylitol containing toothpastes and mouthwashes against Streptococcus mutans (S. mutans) and Lactobacillus (LB) in subjects within the age group of 18 to 22 years at time intervals of 1, 3, and 6 months. Materials and methods One hundred subjects were randomly divided into four groups. Group I: fluoride, group II: chlorhexidine, group III: herbal, group IV: xylitol and instructed to use toothpastes and mouthwashes containing the specific agents. Salivary samples were collected to evaluate the levels of S. mutans and LB at baseline, 1, 3, and 6 months. Bacterial levels were evaluated using caries risk test (CRT) kit. Data were analyzed using analysis of variance and post hoc test. Results During intragroup comparison, S. mutans levels in group I showed statistically significant difference among the four time intervals. On intergroup comparison, S. mutans levels after 6 months for groups I, II, III, and IV were 1.12, 1.16, 1.28, and 1.4 respectively. Conclusion It can be concluded that fluoride, CHX, and xylitol showed a significant reduction in S. mutans and LB count after a time period of 6 months while herbal group did not show a significant reduction in S. mutans and LB count at any intervals. How to cite this article Sam JE, Benin P, Beaulah RH, Gnanaseelan, Krishna L, Raja J. Comparative Evaluation of Antibacterial Efficacy of Four Toothpastes and Mouthwashes against Streptococcus mutans and Lactobacillus: An in vivo Study. J Oper Dent Endod 2016;1(2):60-65.
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Kariminik, Ashraf, and Mohammad-Mahdi Motaghi. "Evaluation of Antimicrobial susceptibility pattern of Streptococcus mutans isolated from dental plaques to chlorhexidine, nanosil and common antibiotics." International Journal of Life Sciences 9, no. 2 (February 10, 2015): 18–21. http://dx.doi.org/10.3126/ijls.v9i2.12040.
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Streptococcus mutans is one of the most important leading causes of dental diseases worldwide and is considered as one of the main causative agent of dental caries. Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems. The aim of this study was to examine susceptibility of Streptococcus mutans isolates to some antibiotics, chlorhexidine and nanosil. The study subjects comprised of caries active individual volunteers attending the outpatient department of different dental college hospitals of Kerman. The saliva sample of 2-5 ml was collected from each individual in sterile capped bottles and were immediately transported to the laboratory and processed for the screening of S. mutans. The specific selective media, Blood agar and Tryptone yeast cysteine media agar were used for the screening and isolation of Streptococcus mutans and incubated anaerobically at 37?C for 48 hrs. Colonies of mutans streptococci were examined under a dissecting microscope and identified by their distinctive colony morphology and biochemical tests .Mutacin production of all isolates were investigated.The isolates were tested for susceptibility to some antibiotics (penicillin, gentamycin, vancomycin, cephalotin), chlorhexidine and nanosil mouthwashes by disc diffusion method. The results showed that the majority of mutacin and non mutacin-producing isolates were more sensitive to the nanosil than antibiotics and chlorhexidine. More extensive research on the use of mouthwashes containing silver nanoparticles is suggested.DOI: http://dx.doi.org/10.3126/ijls.v9i2.12040 International Journal of Life Sciences 9 (2) : 2015; 18-21
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Devinyak, O. T., E. M. Vashkeba-Bitler, M. M. Fizer, I. Yu Stan, Ya I. Deyak, and O. V. Lytvyn. "The assortment analysis of chlorhexidine-containing medicines and procurement data of state and municipal organizations." Clinical pharmacy 25, no. 1 (February 26, 2021): 41–48. http://dx.doi.org/10.24959/cphj.21.1543.
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Chlorhexidine is an effective cationic antiseptic and disinfectant that has been used in medical practice for decades. It is generally recommended to use different concentrations of aqueous or alcoholic solutions of chlorhexidine for different therapeutic purposes.
Objective. To determine the proportions of different chlorhexidine concentrations in medicines purchased by state and municipal organizations and to identify possible issue of chlorhexidine formulations utilization in the suboptimal or insufficient concentrations.
Results. The vast majority of the chlorhexidine-containing medicines found in the State Register of Medicines of Ukraine are 0.05% solutions for external use, 0.05% gels for dental and urological use and vaginal suppositories (pessaries) with 16 mg of chlorhexidine in one dose. While the most common representatives of chlorhexidine-containing medicines abroad are solutions with a concentration of 4% and 2%, as well as mouthwashes with a concentration of 0.12%. The highest share - 96% - among the procurements of chlorhexidine-containing medicines by state and municipal organizations is 0.05% aqueous solution for external use. Instructions for medical use of 0.05% aqueous chlorhexidine solution for external use contain indications that are not supported by international documents: WHO Model List of Essential Medicines and British National Formulary.
Conclusions. The obtained results suggest probable cases of irrational use of chlorhexidine medicines in medical practice in Ukraine. The consequences of such cases are not limited to the failure to achieve therapeutic goals or to the development of nosocomial infection, but includes also the selection of chlorhexidine-resistant strains of microorganisms.
Key words: chlorhexidine; assortment analysis; medicinal procurements study; suboptimal concentration; rational pharmacotherapy
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Pathi, Jayashree, Kumudini Panigrahi, Ipsa Mohapatra, and Ratikanta Tripathy. "A Comparative Assessment of the Antibacterial Efficacy of Licorice Mouth-Rinse with Chlorhexidine on Salivary Streptococcus mutans." Journal of Evolution of Medical and Dental Sciences 10, no. 15 (April 12, 2021): 1042–47. http://dx.doi.org/10.14260/jemds/2021/223.
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BACKGROUND Dental and periodontal diseases are common problems worldwide. Strong association exists between Streptococcus mutans and dental caries. Mouthwashes like chlorohexidine and extracts of medicinal plants like liquorice have antimicrobial properties. The objective of the study was to compare the antimicrobial efficacy of licorice mouth-rinse with chlorhexidine on salivary Streptococcus mutans. METHODS A randomised control trial was undertaken in the department of Public Health Dentistry, in a tertiary care hospital of Bhubaneswar, Odisha. Children of both sexes, aged 7 to 14 years, with high risk of caries and providing willingness were included. The products used were Aqueous and Ethanolic licorice root extract – 15 gm and 375 mg / 10 ml respectively, Chlorhexidine 0.12 %. MIC of the products against Streptococcus mutans was determined. The children were divided into three groups, fifteen in each. Each participant rinsed with 10 ml of the randomly allocated prepared suspension for 1 min. Five saliva samples were collected from each, one pre-rinse and four post-rinse 2 mins, 30 mins, 1 hour and 2 hours after the intervention. Streptococcus mutans colony count and salivary pH was used to study the efficacy of the mouthwashes. RESULTS The study revealed that ethanolic extract of licorice had better antimicrobial efficacy. The efficacy of antimicrobial action of licorice extract at 30 minutes of rinsing and rise in salivary pH by use of both the preparations of licorice was significant as compared to the chlorhexidine group. CONCLUSIONS The antimicrobial and cariostatic efficacy of licorice extracts which was evident in the present study suggests and recommends that licorice can be used as a preventive regimen in clinical practice for diseases of mouth cavity especially dental caries. KEY WORDS Dental Caries, Streptococcus mutans, Licorice, Chlorhexidine, Mouth Wash
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Alsarhan, Mohammed, Hourya Alnofaie, Rawan Ateeq, and Ahmed Almahdy. "The Effect of Chlorhexidine and Listerine® Mouthwashes on the Tensile Strength of Selected Absorbable Sutures: An In Vitro Study." BioMed Research International 2018 (November 13, 2018): 1–8. http://dx.doi.org/10.1155/2018/8531706.
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Background. Suturing plays a critical role in the healing of surgical wounds. The tensile strength of suture materials indicates the ability of the material to withstand stress during knotting and protect the wound during an extended period of healing. Objective. An in vitro study was conducted to determine the effect of two commercially available mouthwashes on the tensile strength and breakage mode of two absorbable intraoral sutures. Materials and Methods. Two common absorbable sutures, Vicryl® and Monocryl®, both with 4-0 and 5-0 gauges were used. A total of 400 specimens were sutured around rubber rods and immersed in three thermostatically controlled experimental conditions: artificial saliva, 0.2% chlorhexidine gluconate (Parodontax® extra), and essential oils-based rinse (Listerine® Zero™), and these were compared to a nonimmersed dry condition. All specimens were stored in an incubator at 37°C. Tensile strengths were assessed after days 1, 3, 7, 10, and 14 of immersion using a universal Instron® testing machine. The maximum load for suture breakage and the location of the point of breakage were assessed. Results. Unlike Monocryl® 4-0, the tensile strength of both gauges of Vicryl® sutures significantly increased in chlorhexidine and Listerine®. There was a significant decrease in the strength for all suture types after day 10, regardless of the immersion solution. Listerine® significantly reduced the tensile strength of Monocryl® 5-0. Conclusion. Oral surgeons and periodontists should be cautious when prescribing commercial mouthwashes for patients relative to their selection of suture materials. However, further studies are needed to understand the molecular changes in sutures when exposed to chemical solutions found in mouthwashes.
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Warad, Shivaraj, and Ravi V. Bhatagunaki. "Effect of different mouth washes as a pre-procedural rinse to combat aerosol contamination– A cross-sectional study." UNIVERSITY JOURNAL OF DENTAL SCIENCES 6, no. 3 (January 11, 2021): 58–62. http://dx.doi.org/10.21276/ujds.2020.6.3.8.
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Background: Ultrasonic scaling is a potent source of aerosol generation in dental offices, thus causing increase risk of bacterial infections. Pre-procedural mouth rinsing has been found effective in reducing the bacterial load of the aerosol produced during the procedure. Aim and objectives: the aim of the present study was to evaluate and compare the efficacy of two different mouthwashes containing Chlorhexidine and Octenidine with distilled water, by using them as preprocedural rinsing agents in reducing the bacterial load of the aerosol produced by ultrasonic scaler. Materials and Methods: 80 subjects aged 18-35years were randomly divided into three groups on the basis of mouth rinses used for preprocedural mouthrinsing - Group 1: Distilled Water (Control), Group 2: 0.2% Chlorhexidine (CHX), Group 3: 0.1% Octenidine. The aerosols were collected on agar plates placed and stabilized on patient’s as well as on operator’s chest. all the agar plates were sent for microbiological analysis to the microbiological laboratory for Colony Forming Unit (CFU) count on the same day of ultrasonic scaling procedure. The data obtained was subjected to the statistical analysis using SPSS software version 20.0. Result: At all locations, the mean CFU was significantly highest in Group I, followed by Group II and Group III. It was observed that aerosol generation on patients was significantly more than operator. Conclusion: In our study 0.1% octenidine was found to be most effective preprocedural mouthwash in reducing the bacterial load in the aerosol produced during ultrasonic scaling followed by 0.2% chlorhexidine and distilled water.
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Mostafa, Basma, and Mai Zakaria. "Comparing pomegranate extract and chlorhexidine mouthwashes in treatment of recurrent intraoral herpes." Journal of The Arab Society for Medical Research 13, no. 1 (2018): 53. http://dx.doi.org/10.4103/jasmr.jasmr_5_18.
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Chye, Rachel Mei Ling, Vittoria Perrotti, Adriano Piattelli, Flavia Iaculli, and Alessandro Quaranta. "Effectiveness of Different Commercial Chlorhexidine-Based Mouthwashes After Periodontal and Implant Surgery." Implant Dentistry 28, no. 1 (February 2019): 74–85. http://dx.doi.org/10.1097/id.0000000000000854.
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