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Journal articles on the topic "Mpb"
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Zhang, Wenfu, Shaohua Gu, Cuicui Wang, Haitao Cheng, and Ge Wang. "Physico-mechanical and joint performance of bamboo veneer products manufactured by mould pressing." BioResources 14, no. 2 (2019): 3823–32. http://dx.doi.org/10.15376/biores.14.2.3823-3832.
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Bamboo can be processed into engineering materials with excellent properties by reasonable processing methods. In this study, the performance of mould-pressed bamboo (MBP) veneer products was examined. The physical mechanical properties and connection properties of MPB were tested, and the application performance of the MPB was analyzed. The results show that MPB has a comprehensive property of high internal bonding and good dimensional stability, and its density and mechanical properties are similar to those of wood dimensional stock. The overall bending strength, bending modulus, and compression strength of MPB were 29.0 MPa, 6.83 GPa, and 15.6 MPa, respectively. While the overall carrying capacity was relatively low, the connection performance of BPM was good. Thus, it can be used as a connector or substructure.
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McFarlane, Bonita L., John R. Parkins, and Sharon Romanowski. "Expert perceptions of media reporting on a large-scale environmental risk issue: insights from mountain pine beetle management in Alberta, Canada." Canadian Journal of Forest Research 46, no. 1 (2016): 1–9. http://dx.doi.org/10.1139/cjfr-2015-0195.
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The media play a key role in communicating risk issues and serve as a link between experts and the public. In this study, we explore experts’ perspectives on the role of the media and media content with respect to a mountain pine beetle (MBP; Dendroctonus ponderosae Hopkins) epidemic in western Canada. We collected data using an internet-based survey of 59 MPB experts in 2009 and 1628 articles from local, regional, and national newspapers from 2000 to 2008. Survey findings show that experts do not have a favourable view of media reporting of MPB. In our media analysis, we found that the media are used effectively by experts to disseminate MPB risk information and to justify a swift response and control measures. Most articles reported information presented to them by government and industry. We conclude that the media played primarily an educational role and gave limited attention to fostering public dialogue on MPB management.
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Amar, Sapir, David Hanelin, and Ilir Agalliu. "Abstract 850: Androgenic alopecia and risks of overall and aggressive prostate cancer: An updated meta-analysis." Cancer Research 84, no. 6_Supplement (2024): 850. http://dx.doi.org/10.1158/1538-7445.am2024-850.
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Abstract Background: Androgenic alopecia, known as male pattern baldness (MPB), is a common hair loss disorder among men. MPB has similar risk factors with prostate cancer (PrCa) including advancing age, family history, and sex hormones. Several studies have examined the associations between MPB and PrCa but the evidence remains unclear. We carried out a meta-analysis of epidemiological studies that examined the relationship between patterns of baldness: frontal, vertex, or both and PrCa risk. Methods: A literature search was performed in October 2022 using PubMed and Web of Science databases for epidemiological studies published between 1/1/2000 and 10/31/2022, which examined the above hypothesis. From each eligible study, relevant data were extracted on study design, population, sample size, prevalence of MPB at various ages, and corresponding associations with risks of total and aggressive PrCa. Pooled relative risks (RR) and corresponding 95% confidence intervals (CI) were calculated using the DerSimonian and Laird random-effects models. Heterogeneity across studies was assessed by I2 statistic. Results: A total of 14 studies: 14,573 cases and 132,434 controls/non-cases were included. The prevalence of MPB increased from 5% to 70% with aging and varied across studies. Vertex MPB at ages 40 years or older was associated with a pooled RR of 1.08 (95%CI 1.00-1.17) of total PrCa, but there was no association with frontal MPB. The onset of MBP at younger ages was also associated with RR=1.10 (95% CI 0.93 - 1.29) of PrCa, although results were not statistically significant. Results were similar when data were stratified by clinical features of PrCa, although there was large heterogeneity in the definition of aggressive PrCa across studies. Conclusions: Men with vertex MPB may have modest increased risk of PrCa. However, the majority of studies were conducted in Caucasian men and they did not evaluate effect modifications by genetic variations in androgen metabolism pathway genes or changes in serum levels of androgens. Further, large well-designed prospective cohort studies are needed to better understand the complex relationship between genetic susceptibility, endogenous hormones, MBP and risk of PrCa. Citation Format: Sapir Amar, David Hanelin, Ilir Agalliu. Androgenic alopecia and risks of overall and aggressive prostate cancer: An updated meta-analysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 850.
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Rankin, L. J., and J. H. Borden. "Competitive interactions between the mountain pine beetle and the pine engraver in lodgepole pine." Canadian Journal of Forest Research 21, no. 7 (1991): 1029–36. http://dx.doi.org/10.1139/x91-141.
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The pine engraver (PE), Ipspini (Say), often coexists with the mountain pine beetle (MPB), Dendroctonusponderosae Hopk., in lodgepole pine, Pinuscontorta var. latifolia Engelm. The PE colonizes the upper bole, while the MPB infests the lower bole. We investigated the hypothesis that interspecific interactions between the PE and the MPB within trees can adversely affect MPB progeny production or survival. In lodgepole pine bark attacked by both species, PE and MPB emergence holes had a strong negative interrelationship, suggesting that high numbers of one species resulted in lower numbers of the other. In the laboratory, attacks by PEs on lodgepole pine logs resulted in significantly decreased numbers of MPB progeny, particularly when MPBs and PEs were allowed to attack logs simultaneously. Reductions in MPB progeny were 92.8 and 96.2% when the ratios of attacking beetles were 100 PE: 50 MPB per square metre and 200 PE: 50 MPB per square metre, respectively, compared with that in control logs with 50 MPB per square metre. In an August field experiment, pheromone-induced attack by the PE on trees just attacked by the MPB resulted in a 72.5% reduction in mean MPB progeny production compared with that in MPB-attacked control trees. Possible reasons for the reduced success of MPB when it and the PE coinhabit the same host may be exploitation competition, interference competition, or introduction of fungal competitors deleterious to the MPB. Induced infestation by PEs of MPB-infested trees may have the potential to reduce or control MPB populations.
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Deng, Hong Wei, Ying Zi Yang, and Xiao Jian Gao. "Properties of Phosphate Cement Mortar Used as Rapid-Repair Material." Advanced Materials Research 250-253 (May 2011): 1752–56. http://dx.doi.org/10.4028/www.scientific.net/amr.250-253.1752.
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In order to determine the optimal proportion of magnesium phosphate cement mortar, the influences of ratio of magnesium phosphate cement-based binder (MPB) to sand (MPB/S), effects of fly ash on fluidity and strength development of MPB mortar, and the compatibility between MPB and traditional concrete and shrinkage of MPB mortar were investigated in this paper. The optimal proportion in this test was: setting adjusting agent of 12%, P/M ratio of 1:2(in weight), MPB/S ratio of 1:1 and FA/S ratio of 15%. The results showed that the MPB mortar met the higher early strength requirement of rapid-repair materials, with compressive strength beyond 50MPa and flexural strength more than 9.1MPa at 3 hours, and at later ages no reduction of strength happened. There was a good compatibility between MPB and traditional concrete. The shrinkage of MPB mortar at 28 days was less than 2.89 × 10-4. Therefore MPB is very suitable for rapid repairing of concrete structures.
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Panthakkal Abdul Muthalif, Mohammed, and Youngson Choe. "Influence of Maleinized Polybutadiene on Adhesive Strength and Toughness of Epoxy Resins." International Journal of Polymer Science 2022 (December 28, 2022): 1–8. http://dx.doi.org/10.1155/2022/9517467.
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This study explored the effect of maleinized polybutadiene (MPB) on the mechanical properties of epoxy resins. Diglycidyl ether of bisphenol-A, an epoxy resin, was modified by incorporating MPB having different molecular weights in order to improve the fracture toughness and peel strength. MPB was mixed with epoxy resin at several concentrations (5, 10, and 15 phr), with the epoxy resin as the major phase and MPB as the minor phase. A comparative study was performed to investigate the influence of MPB on epoxy resins based on their molecular weight difference. Lap shear test results showed that the shear strength of the MPB-modified epoxy resins was superior to that of the neat epoxy resin. At 10 wt% MPB loading, the modified epoxy resin exhibited an 87% enhancement in T-peel strength relative to that of the neat epoxy resin. Moreover, the fracture energy of the modified epoxy system increased proportionally with the amount of MPB in the epoxy matrix. These results indicate that MPB incorporation is a simple and effective method for designing multifunctional epoxy resins, thus facilitating their industrial application in various spheres.
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Pushkarev, Averyan V., Alexey V. Orlov, Sergey L. Znoyko, Vera A. Bragina, and Petr I. Nikitin. "Rapid and Easy-to-Use Method for Accurate Characterization of Target Binding and Kinetics of Magnetic Particle Bioconjugates for Biosensing." Sensors 21, no. 8 (2021): 2802. http://dx.doi.org/10.3390/s21082802.
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The ever-increasing use of magnetic particle bioconjugates (MPB) in biosensors calls for methods of comprehensive characterization of their interaction with targets. Label-free optical sensors commonly used for studying inter-molecular interactions have limited potential for MPB because of their large size and multi-component non-transparent structure. We present an easy-to-use method that requires only three 20-min express measurements to determine the key parameters for selection of optimal MPB for a biosensor: kinetic and equilibrium characteristics, and a fraction of biomolecules on the MPB surface that are capable of active targeting. The method also provides a prognostic dependence of MPB targeting efficiency upon interaction duration and sample volume. These features are possible due to joining a magnetic lateral flow assay, a highly sensitive sensor for MPB detection by the magnetic particle quantification technique, and a novel mathematical model that explicitly describes the MPB-target interactions and does not comprise parameters to be fitted additionally. The method was demonstrated by experiments on MPB targeting of cardiac troponin I and staphylococcal enterotoxin B. The validation by an independent label-free technique of spectral-correlation interferometry showed good correlation between the results obtained by both methods. The presented method can be applied to other targets for faster development and selection of MPB for affinity sensors, analytical technologies, and realization of novel concepts of MPB-based biosensing in vivo.
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Straub, Paul. "MPB and Tissue Expansion." International Society of Hair Restoration Surgery 3, no. 5 (1993): 10–11. http://dx.doi.org/10.33589/3.5.0010.
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Hochfeld, Lara M., Marta Bertolini, David Broadley, et al. "Evidence for a functional interaction of WNT10A and EBF1 in male-pattern baldness." PLOS ONE 16, no. 9 (2021): e0256846. http://dx.doi.org/10.1371/journal.pone.0256846.
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More than 300 genetic risk loci have been identified for male pattern baldness (MPB) but little is known about the exact molecular mechanisms through which the associated variants exert their effects on MPB pathophysiology. Here, we aimed at further elucidating the regulatory architecture of the MPB risk locus on chromosome (chr.) 2q35, where we have previously reported a regulatory effect of the MPB lead variant on the expression of WNT10A. A HaploReg database research for regulatory annotations revealed that the association signal at 2q35 maps to a binding site for the transcription factor EBF1, whose gene is located at a second MPB risk locus on chr. 5q33.3. To investigate a potential interaction between EBF1 and WNT10A during MPB development, we performed in vitro luciferase reporter assays as well as expression analyses and immunofluorescence co-stainings in microdissected human hair follicles. Our experiments confirm that EBF1 activates the WNT10A promoter and that the WNT10A/EBF1 interaction is impacted by the allelic expression of the MPB risk allele at 2q35. Expression analyses across different hair cycle phases and immunhistochemical (co)stainings against WNT10A and EBF1 suggest a predominant relevance of EBF1/WNT10A interaction for hair shaft formation during anagen. Based on these findings we suggest a functional mechanism at the 2q35 risk locus for MPB, where an MPB-risk allele associated reduction in WNT10A promoter activation via EBF1 results in a decrease in WNT10A expression that eventually results in anagen shortening, that is frequently observed in MPB affected hair follicles. To our knowledge, this study is the first follow-up study on MPB that proves functional interaction between two MPB risk loci and sheds light on the underlying pathophysiological mechanism at these loci.
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Echappé, Caroline, Pierre Gernez, Vona Méléder, et al. "Satellite remote sensing reveals a positive impact of living oyster reefs on microalgal biofilm development." Biogeosciences 15, no. 3 (2018): 905–18. http://dx.doi.org/10.5194/bg-15-905-2018.
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Abstract. Satellite remote sensing (RS) is routinely used for the large-scale monitoring of microphytobenthos (MPB) biomass in intertidal mudflats and has greatly improved our knowledge of MPB spatio-temporal variability and its potential drivers. Processes operating on smaller scales however, such as the impact of benthic macrofauna on MPB development, to date remain underinvestigated. In this study, we analysed the influence of wild Crassostrea gigas oyster reefs on MPB biofilm development using multispectral RS. A 30-year time series (1985–2015) combining high-resolution (30 m) Landsat and SPOT data was built in order to explore the relationship between C. gigas reefs and MPB spatial distribution and seasonal dynamics, using the normalized difference vegetation index (NDVI). Emphasis was placed on the analysis of a before–after control-impact (BACI) experiment designed to assess the effect of oyster killing on the surrounding MPB biofilms. Our RS data reveal that the presence of oyster reefs positively affects MPB biofilm development. Analysis of the historical time series first showed the presence of persistent, highly concentrated MPB patches around oyster reefs. This observation was supported by the BACI experiment which showed that killing the oysters (while leaving the physical reef structure, i.e. oyster shells, intact) negatively affected both MPB biofilm biomass and spatial stability around the reef. As such, our results are consistent with the hypothesis of nutrient input as an explanation for the MPB growth-promoting effect of oysters, whereby organic and inorganic matter released through oyster excretion and biodeposition stimulates MPB biomass accumulation. MPB also showed marked seasonal variations in biomass and patch shape, size and degree of aggregation around the oyster reefs. Seasonal variations in biomass, with higher NDVI during spring and autumn, were consistent with those observed on broader scales in other European mudflats. Our study provides the first multi-sensor RS satellite evidence of the promoting and structuring effect of oyster reefs on MPB biofilms.
Dissertations / Theses on the topic "Mpb"
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Saldanha, Rafael Machado. "Estudando a MPB: reflexões sobre a MPB, nova MPB e o que o público entende por isso." reponame:Repositório Institucional do FGV, 2008. http://hdl.handle.net/10438/2618.
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CPDOC2008RafaelMachadoSaldanha.pdf: 426771 bytes, checksum: 35b75e94fe63e25eaeb9a47250a25e8e (MD5)<br>The purpose of this research is to discuss the evolution of the use of the abbreviation MPB (Música Popular Brasileira – Brazilian Popular Music), since its popularization, on 60’s, until the present days, by placing emphasis on its recent ramification as known as 'Nova MPB'. It also tried to understand how its reception by the Brazilian public works, trying to contrast the information gotten in the bibliographical research with the opinion of MPB listeners congregated in an Internet group.<br>Este trabalho pretende discutir a evolução do uso da sigla MPB (Música Popular Brasileira), desde sua popularização, nos anos 60, até os dias de hoje, dando ênfase para sua ramificação recente conhecida como 'Nova MPB'. Também se buscou compreender como se dá a recepção por parte do público brasileiro, procurando contrastar as informações obtidas nas pesquisas bibliográficas com a opinião de ouvintes de MPB reunidos através de um fórum da Internet.
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ALMEIDA, Laís Barros Falcão de. "A MPB em mudança: cartografando a controvérsia da nova MPB." Universidade Federal de Pernambuco, 2016. https://repositorio.ufpe.br/handle/123456789/17438.
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Previous issue date: 2016-02-25<br>FACEPE<br>A Nova MPB surgiu como termo controverso na crítica musical brasileira no início do século XXI para se referir a atores que se apresentaram no contexto de reconfiguração da indústria da música, desencadeado pelas plataformas digitais e o download de músicas pela internet, levantando questionamentos sobre a Música Popular Brasileira (MPB). O objetivo deste estudo foi cartografar a controvérsia da Nova MPB descrevendo e analisando sua rede sociotécnica para indicar as rearticulações que a controvérsia da Nova MPB negocia com a MPB. Trata-se de um estudo teórico e metodológico sobre a MPB, segundo os pressupostos teóricos e os conceitos da Teoria do Ator Rede (TAR), suas articulações possíveis com os estudos de comunicação e música, especificamente com os gêneros musicais, pensados a partir da TAR como constituídos de controvérsias, sendo a Nova MPB uma controvérsia contemporânea da MPB. Seguindo essa perspectiva, foi aplicada a Cartografia das Controvérsias (CC) como metodologia para rastrear e mapear a Nova MPB, identificando os seus atores e analisando suas ações, declarações e opiniões disponibilizadas nas redes digitais online, descrevendo seu surgimento e a abertura de caixas pretas sobre a MPB no que diz respeito ao critério de rotulação de artistas, busca por aprovação de nomes consagrados, o popular, autonomia comercial e artística, a qualidade musical, a aceitação e negação de rótulos e a questão do novo na MPB. A partir da descrição da controvérsia, foi elaborado um relato crítico sobre a Nova MPB, descrevendo e analisando suas possíveis estabilizações enquanto rede sociotécnica, dando o destaque necessário aos principais atores não-humanos da Nova MPB e as mudanças na MPB com a incorporação da mediação técnica da internet no modo de operação independente na Nova MPB e de produções artísticas que trazem entre suas possibilidades estéticas atravessamentos do indie rock e da música pop, além da noção de canção expandida. Por fim, a TAR indicou os rumos possíveis de pesquisa para os estudos da MPB na contemporaneidade e como as controvérsias são as principais mobilizadoras nos processos de renovação e transformação de gêneros musicais.<br>The New MPB emerged as a controversial term in Brazilian music critic in the early twenty-first century to refer to actors who performed in the context of the music industry reconfiguration, triggered by digital platforms and downloading music on the internet, raising questions about the Brazilian Popular Music (MPB). The objective of this study was to map the controversy of New MPB describing and analyzing its socio-technical network to indicate the rearticulations that New MPB controversy negotiates with the MPB. This is a theoretical and methodological study of the MPB, according to the theoretical assumptions and concepts of Actor-Network Theory (ANT), their possible links with the communication and music studies, specifically with musical genres, thought from the ANT as consisting of disputes, the New MPB is a contemporary controversy of MPB. Following this perspective, it was applied the Cartography of Controversies (CC) as a methodology to track and map the New MPB, identifying their actors and analyzing their actions, statements and opinions provided in the online digital networks, describing their appearance and the opening of black boxes on MPB about the labeling criteria for artists, search for approval in established names, the popular, commercial and artistic autonomy, the musical quality, acceptance and denial of labels and the issue of new in the MPB. From the description of the controversy, a critical account of the New MPB was prepared, describing and analyzing its possible stabilization as socio-technical network, giving the necessary prominence to the main non-human actors of the New MPB and the changes in MPB with the incorporation of internet technical mediation in the independent mode of operation in the New MPB and artistic productions that bring between their aesthetic possibilities the penetrations of indie rock and pop music, beyond the notion of expanded song. Finally, the ANT indicated the possible direction for researches in the MPB studies nowadays and how controversies are the main mobilizing the processes of renewal and transformation of musical genres
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Guimarães, Selene Santa Rosa Macieira e. "O discurso amoroso do (NA) MPB." [s.n.], 1989. http://repositorio.unicamp.br/jspui/handle/REPOSIP/270672.
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Previous issue date: 1989<br>Resumo: Este trabalho tem como objetivo o estudo do Discurso Amoroso da Música Popular Brasileira dentro da metodologia proposta pela Análise
de Discurso. Inicialmente, abordo a questão da seleção do corpus através de sucessivas e significativas filtragens que este se delimita. Estudando, então, discursivamente, as letras de sambas líricos-amorosos e sambas-canções, de Ataulfo Alves e Lupiscínio Rodrigues, procuro investigar como através da figura da mulher, amoroso, presença constante e ausência inevitável, se constrói identidade do sujeito amoroso. Na tentativa de deslocar o t.ema amoroso como "mera banalidade", lugar tão somente do mesmo, da reprodução, e demonstrá-lo, ao contrário, como possibilidade de ruptura, de transformação, busco descrever, a partir de determinados conceitos teóricos estabelecidos pela Análise de Discurso, a relação que se pode observar entre discurso amoroso da MPB (enquanto discurso pertencente a um grupo social dominado) e discurso cientifico (enquanto discurso pertencente a um grupo social dominante) isto porque, revestindo-se o tema amoroso de uma função estruturante, importa sobretudo ao discurso amoroso o compromisso da explicação, da descrição, da definição, enfim, da organização de um "eu", identidade representativa de uma cultura dominada. É ainda no quadro dessa preocupação a respeito da relação de forças (conflito ideológico) entre grupos sociais distintos, constitutiva de seus discursos, que se insere, e se desenvolve, a discussão sobre as condições sócio-históricas de surgimento do samba e sambacanção; pois que a determinação do que vem a ser orodução popular, de um grupo a outro, participa sianificativamente do dizer amoroso da MPB<br>Mestrado<br>Mestre em Linguística
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Nunes, Célia Regina. "O ciúme na MPB: uma análise retórica." Pontifícia Universidade Católica de São Paulo, 2007. https://tede2.pucsp.br/handle/handle/14436.
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Previous issue date: 2007-05-17<br>This work shows us the importance of reading and its possibilities. We worked with sociointeract reading and one of the possibilities of facilitator text to develop the reading process in school. We brought to discussion the use of MPB like important text and appropriated to develop reader learners.
We used as theoretical referential Aristotle s passion and a brief concept of old rhetoric to analyze with the new Perelman and Tyteca´s rhetoric. We detached the use of argument and metaphor in the new rhetoric and found, by means of jealousy´s representation, this presence in MPB songs of 70 s.
Finally, we analyzed two MPB songs and pointed the jealousy s presence by means of passion. We followed the rhetoric s bases to identify persuasive speech in MPB and we believe that the argument be able to persuade a lot of types of audience and for phatos be reached it´s just invest in the ethos´ constitution<br>Essa dissertação trata da importância da leitura e aborda uma das suas nuances como colaboradora na formação do indivíduo leitor. Optamos por trabalhar com a leitura sócio-interativa e a MPB como uma das possibilidades de texto facilitador para desenvolver o processo de leitura: trazemos para a discussão o uso da canção como texto significativo e apropriado para desenvolver a leitura.
Utilizamos como referencial teórico as paixões de Aristóteles e um breve conceito da antiga retórica, bem como e traçamos um paralelo com a nova retórica de Perelman e Tyteca. Destacamos o papel do uso do argumento e da metáfora na nova retórica e localizamos, por meio da representação do ciúme, esta presença nas canções de MPB da década de 70.
Por fim, analisamos duas canções e apontamos a presença do ciúme por meio das paixões. Seguimos os fundamentos da retórica para identificar o discurso persuasivo na MPB. Concluímos que o argumento é capaz de persuadir os mais diversos auditórios. Para que o pathos seja atingido, é só investir na constituição do ethos
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Pinheiro, Luiz Roberto Martins. "Ruptura e continuidade na MPB: a questão da linha evolutiva." reponame:Repositório Institucional da UFSC, 1992. http://repositorio.ufsc.br/xmlui/handle/123456789/76875.
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91782.pdf: 2553491 bytes, checksum: 9717ac004c2d21dc4c22d26d3c9e713f (MD5)<br>O objetivo central deste trabalho está em interpretar o sentido da discursividade nativa Bossa Nova (BN) e Tropicalista (TROPI) no que tange à evolução musical popular associado à ruptura estética moderna. Para obter este objetivo utilizo-me de duas frentes: a primeira, examina a topologia discursiva, enquanto a segunda mergulha na feitura das canções. A obra divide-se em quatro partes. Na primeira, faço um apanhado de umas obras musicológicas para demonstrar o atual estágio deste assunto em ciências humanas. Na segunda verifico a discursividade em seus nexos com a ideologia de construção nacional do Brasil através da música. Na terceira parte realizo um exame semântico de algumas canções do movimento BN-TROPI, tentanto apontar para os momentos de entrelaçamento destes com esquemas estilísticos tradicionais. Por fim, a quarta parte é a conclusão, onde faço um rápido arremate de algumas idéias levantadas nas partes anteriores.
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Lunardi, Rafaela. "Preparando a tinta, enfeitando a praça: o papel da MPB na \'abertura política\' brasileira (1977-1984)." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/8/8138/tde-03112016-150306/.
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O trabalho procura analisar o papel da Música Popular Brasileira (MPB) nos eventos de protesto no período final do Regime Militar, entre 1977 a 1984. Nesse momento, a MPB esteve presente em shows, comícios, TV, rádios e em caminhões de som, embalando manifestações públicas contrárias ao Regime e campanhas eleitorais promovidas por estudantes, partidos de oposição, nos quais destacaram-se o MDB, posteriormente PMDB, PT e PDT, movimentos sindicais, movimentos de bairro, pela Anistia e pelas Diretas Já. É possível constatar que muitos artistas, como Elis Regina, Chico Buarque, Caetano Veloso, Gonzaguinha, Gilberto Gil, entre outros do elenco já consagrado de MPB, estiveram presentes nessas manifestações. Nosso objetivo é compreender as atuações desses artistas como agentes políticos, suas possíveis ligações com os partidos de oposição e o papel das canções na ressignificação dos valores políticos e na construção de um sentido de pertencimento coletivo na oposição ao Regime Militar.<br>The paper analyzes the role of Popular Brazilian Music (MPB) in protest events in the final period of the Military Regime from 1977 to 1984. At that time, the MPB was present at concerts, rallies, TV, radio and sound trucks, cradling public demonstrations against the regime and electoral campaigns by students , opposition parties, in which stood out the MDB later PMDB, PT and PDT, labor movements, neighborhood movements , by Anistia and the Diretas Já. You can see that many artists like Elis Regina, Chico Buarque, Caetano Veloso, Gonzaguinha, Gilberto Gil, among other already established cast of MPB, attended these events. Our goal is to understand the performances of these artists as political agents, their possible links with the opposition parties and the role of songs in reframing of political values and building a sense of collective belonging in opposition to the Military Regime.
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MELLO, THIAGO DE ANA RIBEIRO GOMES THIAGO DE. "FROM THE PEOPLE´S MPB TO THE COMMUNITIES IN POPULAR MUSIC." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2007. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=11693@1.
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COORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR<br>O trabalho compara concepções de música popular, nação e
camadas
populares desenvolvidas em dois períodos da música popular
brasileira: o de
meados dos anos 1960, em que se construiu a idéia de MPB, e
o atual,
caracterizado por sonoridades oriundas das periferias de
grandes cidades, muitas
vezes denominadas comunidades por seus habitantes. Partindo
das categorias
musicais de Mário de Andrade criadas na década de 1920,
analiso o contexto de
formação da MPB como um momento de desenvolvimento do
populário, em
que se propôs uma estética que expressasse musicalmente o
Brasil. Em 67, a
Tropicália, questionando os fundamentos do projeto nacional-
popular, ensejou
outra proposta de representação do país, ao reunir
repertórios locais e
globais. Contemplando mudanças nas interpretações sobre
música popular,
nação e camadas populares, contrasto a produção da MPB dos
anos 60 com a
obra mais recente de Chico Buarque, o Cd Carioca, de 2006,
através de sua
assimilação de ritmos e segmentos da cena musical
brasileira contemporânea,
especialmente o hip-hop, o manguebeat e os sons eletrônicos.<br>This thesis compares conceptions of popular music, nation
and popular
layers developed in two periods of the Popular Brazilian
Music(MPB): The
one in the middles of the 60´s, in which the idea of MPB
was built, and the
current days, characterized by sonorities originating from
the suburbs of big cities,
so-called communities for his inhabitants. Leaving from the
musical categories
of Mário de Andrade created in the decade of 1920, I
analyse the context of
formation of the MPB like a moment of development of the
populário, in
which there was proposed an aesthetics that was
representing musically Brazil. In
67, while questioning the bases of the popular-national
project, the Tropicália
provided with an opportunity for another proposal of
representation of the
country, while joining locals and globals repertoires.
Contemplating the
changes in conceptions of popular music, nation and popular
layers, I
contrast the MPB of the 60´s with the most recent
production of Chico Buarque,
the 2006-year-old Cd Carioca, through his relation with
rhythms and segments of
the musical Brazilian contemporary scene, specially the hip-
hop, the
manguebeat and the electronic sounds.
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Almeida, Mariangela Ribeiro de. "A canção como narrativa : o discurso social na MPB (1965-1975)." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/278914.
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Orientador: Marcelo Siqueira Ridenti<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas<br>Made available in DSpace on 2018-08-04T03:31:27Z (GMT). No. of bitstreams: 1
Almeida_MariangelaRibeirode_M.pdf: 5407724 bytes, checksum: 57716b19b42faf92e17e68b92d51098e (MD5)
Previous issue date: 2005<br>Resumo: Nossa dissertação tem como objeto o discurso social da canção brasileira que foi produzida nas décadas de 1960 e 1970. Por tennos o objetivo de apreender, através da canção, as tensões presentes nas relações sociais do periodo em questão, optamos por trabalhar com as canções apresentadas nos Festivais de Música Popular Brasileira. Isso porque estes eventos tiveram um sucesso considerável e uma divulgação de longo alcance, na medida em que eram vinculados pelas emissoras de televisão. Buscar-se-á entender, pela análise das letras das canções finalistas dos festivais, quem fala através da música popular brasileira, de quais
fOl111aSesta obra de cultura constrói um detenninado discurso e até que ponto este discurso torna-se um possível retrato de um tempo<br>Abstract: Our work has as object the social speech of the brazilian song that was produced in the decades of 1960 and 1970. To tenns the objective to apprehend, through the song, the tensions gifts in the social relations of the period in question, we opt to working with the songs presented in the FestivaIs of Brazilian Popular Music. This because these events had had a considerable success and a long-range spreading, in the measure where they were tied by the television senders. One will search to understand, for the analysis of the letters of the
finalistas songs of the festivaIs, who speaks through Brazilian popular music, of which this workmanship of culture constructs one definitive speech and until point this speech becomes a possible picture of a time<br>Mestrado<br>Mestre em Sociologia
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Gatti, Vanessa Vilas Boas. "Súditos da rebelião: estrutura de sentimento da Nova MPB (2009-2015)." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/8/8132/tde-22122015-110930/.
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Este trabalho analisa a inserção de um grupo de músicos e compositores que surge no final da década de 2000 em São Paulo, denominado Nova MPB, num arranjo de forças que constitui o campo da música popular brasileira. Trata-se de caracterizar a estrutura de sentimento, definindo princípios implícitos e explícitos que permeiam a formação da Nova MPB, cristalizada na sua produção musical. Partindo do pressuposto de que a produção musical é uma produção social, o enredamento que sofrem esses artistas, tanto ao se inserirem num arranjo de forças em curso, quanto nas relações que mantém internas ao grupo, tem efeitos nos seus posicionamentos e composições. O objetivo deste trabalho é problematizar os processos sociais que envolvem a produção musical deste grupo enfocado. Dadas as novas formas de produção, distribuição e divulgação arranjadas pelos artistas, valendo-se de avanços tecnológicos, a Nova MPB colocase como protagonista de uma nova produção musical independente, de maneira autônoma e empreendedora. Os artistas formam uma rede colaborativa abrindo caminhos alternativos aos instituídos pela indústria fonográfica. Ademais, o pertencimento à fração artística da classe média intelectualizada e a proximidade da sonoridade de suas composições, ainda que com algumas exceções, reforçam os valores e princípios que conformam o grupo. Os artistas da Nova MPB se relacionam com a tradição da música brasileira de forma peculiar, se aproximando de figuras consagradas e enaltecendo nomes e momentos da história da música popular brasileira que foram obscurecidos pela atuação da indústria fonográfica. Os novos artistas intentam reescrever a história da música brasileira à sua maneira, incorporando gêneros e nomes antes desconsiderados. Por meio de homenagens, parcerias e releituras, eles se aproximam de figuras icônicas, consagradas ou em vias de consagração. A exaltação de momentos do passado dessa história pode ser interpretada como um dos princípios implícitos que compõe a estrutura de sentimento da Nova MPB.<br>This study aims at analyzing the insertion of a group of Brazilian musicians and composers, collectively known as Nova MPB, in the popular music field, understood here as a power structure. Our purpose is to understand the processes of collective construction of the symbolic positions within this field. The structure of feeling of the Nova MPB group is characterized by both the explicitly and the implicitly principles that guide its formation, which is crystalized in the musical production. Taking for granted that any musical production is a social production, we consider the artists suffer an entanglement as they are inserted in an undergoing power arrangement and as they build relations inside of the group. This entanglement affects their positioning and their compositions. The purpose of this study is to problematize the social processes involved in the musical production of the Nova MPB. Given the new forms of production, distribution, and diffusion proposed by the artists with the help of the new technologies, the Nova MPB, in an autonomous and entrepreneurial way, places itself as the protagonist of a new and independent musical production. A collaborative web is formed as the artists try to find alternatives to the imperatives of the phonographic industry. Although there are a few exceptions, the fact that the artists in general come from an intellectualized middle class and have a similar music style helps the creation of this notion of group. The Nova MPB artists have a peculiar relationship with the Brazilian music: they approach famous and recognized artists, as well as they praise obscure artists and musical movements that the phonographic industry has despised. The new artists intend to rewrite their history by means of the incorporation of long forgotten genres and artists. They approach the already renowned artists and the ascending ones as they organize tributes, produce new versions of their songs, and propose partnerships. The celebration of past moments of the Brazilian popular music history can be interpreted as an implicit principle that compose the structure of feeling of the Nova MPB.
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Dantas, Laura Figueiredo. "O canônico em xeque na MPB: processos de legitimação e ideário de modernidade." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/27/27158/tde-13032017-100118/.
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O ideário modernista de construção de uma identidade nacional no Brasil reverberou no âmbito político-ideológico dos anos 1960. Com o advento dos festivais de música televisionados, o processo de legitimação da sigla MPB perante a audiência estabeleceu um padrão estetizante, a partir de uma cadeia de instituições a serviço das classes dominantes. A edificação de uma MPB tornada canônica a partir da segunda metade do século XX parece resultar do que o historiador Eric Hobsbawn chamou de \"tradição inventada\", em que o sentido de superioridade das elites, inculcado nas demais classes, foi, assim, absorvido pela maioria. A presente pesquisa pretende observar de que maneira as instituições e os agentes culturais atuaram no processo de elitização das ideias de vanguarda que nortearam a MPB, e como, nos dias atuais, a perspectiva de \'evolução\' da sigla é entendida pelos agentes/instituições que, neste início de século, passaram a legitimar a Nova MPB. Esta pesquisa traça, assim, um pequeno esboço do ideário de modernidade da sigla, observando a sua trajetória paradigmática enquanto expressão simbólica de uma identidade nacional.<br>The modernist ideal of building a national identity in Brazil reverberated in the political-ideological context of the 1960s with the advent of televised music festivals, the process of legitimation of MPB acronym before the hearing established an esthetic standard, from a chain institutions in the service of the dominant classes. The building of a made canonical MPB from the second half of the twentieth century seems to result from the historian Eric Hobsbawm called \"invented tradition\" in the sense of superiority of elites, inculcated in other classes, it was thus absorbed by majority. This research aims to observe how the institutions and cultural agents acted in the gentrification process of cutting-edge ideas that guided the MPB, and how, today, the prospect of \'evolution\' of the acronym is understood by agents / institutions the start of this century began to legitimize the new MPB. This research draws thus a small outline abbreviation of modernity of ideas, observing its paradigmatic trajectory as a symbolic expression of national identity.
Books on the topic "Mpb"
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Rego, Enylton José de Sá. MPB, contemporary Brazilian popular music. Latin American Institute, University of New Mexico, 1985.
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Albin, Ricardo Cravo. MPB, a alma do Brasil. Instituto Cultural Cravo Albin, 2009.
Find full textBook chapters on the topic "Mpb"
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"Farewell to MPB." In Brazilian Popular Music and Citizenship. Duke University Press, 2020. http://dx.doi.org/10.1515/9780822393603-005.
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Sandroni, Carlos. "Farewell to MPB." In Brazilian Popular Music and Citizenship. Duke University Press, 2011. http://dx.doi.org/10.1215/9780822393603-004.
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Sandroni, Carlos. "Farewell to MPB." In Brazilian Popular Music and Citizenship. Duke University Press, 2011. http://dx.doi.org/10.2307/j.ctv120qsnp.7.
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"CHAPTER SEVEN. Food Chains and Omnivory." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.103.
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"CHAPTER EIGHT. More Modules." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.123.
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"CHAPTER NINE. Coupling Modules in Space: A Landscape Theory." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.145.
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"CHAPTER TEN. Classic Food Web Theory." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.170.
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"CHAPTER ELEVEN. Adding the Ecosystem." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.189.
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"CHAPTER TWO. A Primer for Dynamical Systems." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.20.
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"CHAPTER TWELVE. Food Webs as Complex Adaptive Systems." In Food Webs (MPB-50). Princeton University Press, 2011. http://dx.doi.org/10.1515/9781400840687.201.
Full textConference papers on the topic "Mpb"
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Dreze, C., A. Waksberg, and B. W. Davis. "Schottky Diode Detector Research At MPB Technologies Inc." In 1986 Quebec Symposium, edited by Jerald A. R. Izatt. SPIE, 1986. http://dx.doi.org/10.1117/12.938821.
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Han, Xing, Yuzhuo Fu, and Jiang Jiang. "Reconfigurable MPB combined with cache coherence protocol in many-core." In 2016 IEEE Advanced Information Management, Communicates, Electronic and Automation Control Conference (IMCEC). IEEE, 2016. http://dx.doi.org/10.1109/imcec.2016.7867239.
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Sreedurga, Gogulapati, Mayank Ratan Bhardwaj, and Yadati Narahari. "Maxmin Participatory Budgeting." In Thirty-First International Joint Conference on Artificial Intelligence {IJCAI-22}. International Joint Conferences on Artificial Intelligence Organization, 2022. http://dx.doi.org/10.24963/ijcai.2022/70.
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Participatory Budgeting (PB) is a popular voting method by which a limited budget is divided among a set of projects, based on the preferences of voters over the projects. PB is broadly categorised as divisible PB (if the projects are fractionally implementable) and indivisible PB (if the projects are atomic). Egalitarianism, an important objective in PB, has not received much attention in the context of indivisible PB. This paper addresses this gap through a detailed study of a natural egalitarian rule, Maxmin Participatory Budgeting (MPB), in the context of indivisible PB. Our study is in two parts: (1) computational (2) axiomatic. In the first part, we prove that MPB is computationally hard and give pseudo-polynomial time and polynomial-time algorithms when parameterized by certain well-motivated parameters. We propose an algorithm that achieves for MPB, additive approximation guarantees for restricted spaces of instances and empirically show that our algorithm in fact gives exact optimal solutions on real-world PB datasets. We also establish an upper bound on the approximation ratio achievable for MPB by the family of exhaustive strategy-proof PB algorithms. In the second part, we undertake an axiomatic study of the MPB rule by generalizing known axioms in the literature. Our study leads to the proposal of a new axiom, maximal coverage, which captures fairness aspects. We prove that MPB satisfies maximal coverage.
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Alrashdan, Mohd H. S., Azrul Azlan Hamzah, and Burhanuddin Yeop Majlis. "RF sputtered PZT thin film at MPB for piezoelectric harvester devices." In 2015 IEEE Regional Symposium on Micro and Nanoelectronics (RSM). IEEE, 2015. http://dx.doi.org/10.1109/rsm.2015.7355018.
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Pandey, Adityanarayan H., Kumarswamy Miriyala, Pravin Varade, N. Shara Sowmya, Ajit R. Kulkarni, and N. Venkataramani. "(Na0.8K0.2)0.5Bi0.5TiO3 thin film: A Pb-free MPB composition with large piezoelectricity." In DAE SOLID STATE PHYSICS SYMPOSIUM 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0016687.
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"Analysis of Substorms Related to Strong MPB at Panagjurishte Station in 2022." In Sixteenth Workshop Solar Influences on the Magnetosphere, Ionosphere and Atmosphere. Space Research and Technology Institute, Bulgarian Academy of Sciences, Sofia Bulgaria, 2024. https://doi.org/10.31401/ws.2024.proc.08.
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Odarenko, E. N., Y. V. Sashkova, E. N. Odarenko, A. A. Shmat'ko, and N. G. Shevchenko. "Analysis of Slow Wave Modes in Modified Photonic Crystal Waveguides Using the MPB Package." In 2018 IEEE 17th International Conference on Mathematical Methods in Electromagnetic Theory (MMET). IEEE, 2018. http://dx.doi.org/10.1109/mmet.2018.8460468.
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Sekhar, K. S. K. R. Chandra, M. L. V. Mahesh, and Tirupathi Patri. "Impact of ac frequency on polarization in Sn-substituted NBT-BT ceramics near MPB." In 3RD PROCESS SYSTEMS ENGINEERING & SAFETY (PROSES) SYMPOSIUM 2023. AIP Publishing, 2025. https://doi.org/10.1063/5.0248266.
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Isaifan, Dina Jamal, and Yousra Suleiman. "Quantifying Biomass of Microphytobenthos in sediments of Mangroves in the east coast of Qatar." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0061.
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Mangroves, Avicennia marina, are highly productive coastal ecosystems with capacity to store carbon within plants and in sediments. Micropytobenthos (MPB) in the sediments also fix carbon and play a significant role in carbon burial. However, there is paucity of information on the role of MPB in coastal carbon budget. We quantified the biomass of MPB as an important carbon pool in the mangrove of Al Thakhira, located at the east coast of Qatar. Sediments at different tidal levels namely, supratidal, intertidal, and subtidal were collected and analyzed for grain size, chlorophyll (a), total carbon, and inorganic carbon contents. Results indicated that sand was the dominant species (60%), followed by silt (39%) and clay (1%) at all tidal levels. While the supratidal level had significantly higher silty sand content, silt dominated the intertidal levels. Moreover, chlorophyll (a) was significantly influenced by tidal levels with highest levels in the subtidal level sediments, where mangroves grow extensively. Results also demonstrated that as we move towards the intertidal zone, the total carbon content in sediments gets higher. Finally, chlorophyll (a) and TOC% were positively associated (r=0.643) in all tidal zones. As we move towards the mangrove subtidal growth area, the total carbon content in sediments gets higher. This work recommends that mangrove forests in Qatar be protected by special sanctuaries and law-enforcement to maintain this natural and dynamic blue carbon ecosystem.
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Pradhan, Lagen kumr, Rabichandra Pandey, and Manoranjan Kar. "Existence of multi crystallographic phase in BNT-BTO solid solution near morphotropic phase boundary (MPB)." In DAE SOLID STATE PHYSICS SYMPOSIUM 2016. Author(s), 2017. http://dx.doi.org/10.1063/1.4980370.
Full textReports on the topic "Mpb"
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Nance, Douglas V. Practical Photonic Bandgap Calculations using MPB. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada570980.
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Mohr, Michelle, Jennifer Cribbs, Joan Dudney, et al. Inventory of white pine blister rust, fire, and bark beetles in sugar pine at Yosemite National Park. National Park Service, 2025. https://doi.org/10.36967/2308879.
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Invasive white pine blister rust (Cronartium ribicola, WPBR) threatens white pine populations throughout North America. Sugar pine (Pinus lambertiana), for example, has been declining in Sequoia and King Canyon National Parks (SEKI) due to WPBR, as well as other mortality agents, including fire, drought, and mountain pine beetle (Dendroctonus ponderosae, MPB). Whether these declines reflect population trends farther north in Yosemite National Park, however, is largely unknown. To this end, we surveyed 56 plots between June and October, 2023 in Yosemite. To test whether fire impacted WPBR or sugar pine recruitment, 23 plots were established in the Rim Fire footprint. Average extent (% of plots with ? 1 infection) and infection rate (% of all live stems with ? 1 infection) was 48.2% and 3.7%, respectively, though the extent was much lower in the Rim Fire plots (26.1%) compared to the primary plots (63.6%). Our generalized linear mixed models of infection rate suggested that fire and the presence of alternate hosts were important correlates of WPBR. As fire severity increased, WPBR infections declined, suggesting that high severity fire may dampen infection rates. Additionally, MPB was typically found in larger diameter stems, and the extent was higher in primary plots (54.6%) compared to the Rim Fire plots (30.4%). Recent surveys from SEKI show that sugar pine and WPBR infection rates are declining, likely due to complex interactions with drought, MPB, and fire. Comparable infection rates in Yosemite suggest that both sugar pine and WPBR may be following a similar trajectory. Though long-term monitoring is needed to quantify trends, our results underscore that restoration will help ensure the persistence of this ecologically important species.
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Gilbert, Dominique, Brett Hayhurst, Sarah Grubisich, et al. A bellwether for microplastic in wetland catchments in the Great Lakes region. Engineer Research and Development Center (U.S.), 2025. https://doi.org/10.21079/11681/49795.
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This study is intended as a bellwether for the occurrence of microplastics (MPs) in Great Lakes wetlands. In 2020, sediment, surface water, and atmospheric deposition samples were collected from wetland catchments in or near five National Wildlife Refuges (NWRs) in the Great Lakes region: Horicon-WI, Seney-MI, Shiawassee-MI, Ottawa- OH, and Montezuma-NY. Sediment and surface water samples were taken from river, stream, and canal inflows and outflows to and from wetland areas. Atmospheric deposition samples were collected in carboys placed near established rain gauges. These sample sites were chosen as indicators of MP deposition into and out of the region’s wetland systems. MPs were extracted from each sample, enumerated, and categorized by particle morphology and polymer type. Average MP particle abundances in the sediment and surface water samples ranged from 344 to 538 particles kg-1 (dry weight) and 2–68 particles m-3, respectively. Atmospheric MP deposition ranged from 5.8 to 22.6 particles m-2 d-1. Fibers were the most abundant MP particle type found in each sample type (sediment, surface water, and atmospheric deposition), followed by fragments. These results suggest that input and retention of MPs are pervasive in the Great Lakes region and surrounding wetland areas.
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Stute, Petra, Susanne Theis, Argy Kolokythas, et al. Systematic review on the influence of progestogens on the endometrium. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2023. http://dx.doi.org/10.37766/inplasy2023.6.0028.
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Review question / Objective: The objective of this systematic review is to summarize published data from randomized controlled trials regarding the influence of a combined menopausal hormone therapy on the endometrium with specific focus on endometrial hyperplasia and endometrial cancer incidence. Eligibility criteria: The following progestagens will be investigated: Norethisterone (acetate) = NET(A), Dienogest = DNG, Dydrogesterone = DYD, Micronized Progesterone = MP, Drospirenone = DRSP, Levonorgestrel = LNG, Cyproterone acetate = CPA, Medroxyprogesterone acetate = MPA, Chlormadinone acetate = CMAExogenously administered on oral, transdermal or vaginal route; duration of study at least 3 months; in humans.
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Lajoie, John. PHENIX Muon Piston Calorimeter (MPC) APD and Prototype MPC Extension (MPC-EX) Tests. Office of Scientific and Technical Information (OSTI), 2013. http://dx.doi.org/10.2172/1128068.
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Han, D., and T. Jones. MPI Profiling. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/15014654.
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St., M., and M. Rose. Identification MIB. RFC Editor, 1993. http://dx.doi.org/10.17487/rfc1414.
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Smith, R., F. Wright, T. Hastings, S. Zilles, and J. Gyllenskog. Printer MIB. RFC Editor, 1995. http://dx.doi.org/10.17487/rfc1759.
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ภู่ทอง, ทรงจันทร์, กิตตินันท์ โกมลภิส, อุมาพร พิมพิทักษ์ та อนุมาศ บัวเขียว. การพัฒนาวิธีเอนไซม์ลิงก์อิมมิวโนซอร์เบนต์แอสเสย์สำหรับตรวจเคซีนจากวัว : รายงานการวิจัยฉบับสมบูรณ์. สถาบันวิจัยเทคโนโลยีชีวภาพและวิศวกรรมพันธุศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2018. https://doi.org/10.58837/chula.res.2018.98.
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น้ำนมวัวเป็นอาหารหลักที่สำคัญชนิดหนึ่งสำหรับทั้งเด็กและผู้ใหญ่ อย่างไรก็ตามมีการพบว่ามีเด็กบางคนไม่สามารถบริโภคน้ำนมวัวได้เนื่องจากมีอาการแพ้ ซึ่งมีสาเหตุส่วนใหญ่มาจากการแพ้เคซีน (casein, CN) ที่พบมากถึง 80% ของโปรตีนในน้ำนม ดังนั้นผู้บริโภคกลุ่มนี้ต้องหลีกเลี่ยงการบริโภคน้ำนมวัวหรืออาหารที่มีน้ำนมวัวเป็นส่วนผสม ดังนั้นในงานวิจัยนี้จึงมีวัตถุประสงค์เพื่อจะสร้างมอนอโคลนที่สามารถผลิตมอนอโคลนอลแอนติบอดี (Mab) ต่อเคซีนจากวัว ที่มีลักษณะสมบัติเหมาะสมสำหรับนำมาใช้เตรียมชุดตรวจเคซีน โดยได้ทำการฉีดกระตุ้นหนูทดลองและเตรียมเซลล์ไฮบริโดมาด้วยวิธีการหลอมรวมเซลล์ที่ใช้กันทั่วไป พบว่าสามารถเตรียม Mab ได้ทั้งสิ้น 60 มอนอโคลน จากเซลล์หลุมต้นกำเนิด 13 หลุม จากการศึกษาลักษณะสมบัติพบว่า Mab มีไอโชไทป์เป็น IgG₁ 46 โคลน IgG₂a 5 โคลน และ IgM 9 โคลน แยกเป็นกลุ่มได้เป็น 7 กลุ่มตามความจำเพาะในการจับกับเคซีนชนิดต่าง ๆ ได้แก่ CN, αs-CN, β-CN, k-CN รวมทั้งแลกโตโกลบูลินและโอวัลบูมิน จึงได้ทำการคัดเลือก Mab มา 10 ตัว ที่จับ CN ได้แต่ไม่จับกับแลกโตโกลบูลินและโอวัลบูมิน ไปศึกษาความจำเพาะของ Mab ต่ออิพิโทปที่ต่างกันบนโมเลกุลของ CN พบว่ามี Mab 8 ตัวที่อาจจับกับ CN ที่อิพิโทปต่างกันได้แก่ CN1F4, CN3F4, CN5F4, CN10F4, CN11F4, CN12F4, CN16F4 และ CN20F4 นำ Mab ทั้ง 8 ตัวมาทดสอบความไวในการตรวจเคซีนจากวัวด้วยวิธี indirect enzyme-linked immunosorbent assay พบว่ามีค่าความเข้มข้นในการยับยั้งที่ 50% (IC₅₀) อยู่ในช่วง 6.1-21.5 นาโนกรัมต่อมิลลิลิตร โดย Mab CN1F4 มีความไวสูงสุดซึ่งมีค่าขีดจำกัดในการตรวจและ IC₅₀ เท่ากับ 2.46±0.01 และ 17.01 ±4.29 ไมโครกรัมต่อมิลลิลิตร ตามลำดับ ดังนั้นจึงเลือก Mab CN1F4 สำหรับการศึกษาต่อไป