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Journal articles on the topic 'MRC5 Cell'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Kim, Ji-Young, Mi-Jin An, Geun-Seup Shin, et al. "Mercury Chloride but Not Lead Acetate Causes Apoptotic Cell Death in Human Lung Fibroblast MRC5 Cells via Regulation of Cell Cycle Progression." International Journal of Molecular Sciences 22, no. 5 (2021): 2494. http://dx.doi.org/10.3390/ijms22052494.

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Heavy metals are important for various biological systems, but, in excess, they pose a serious risk to human health. Heavy metals are commonly used in consumer and industrial products. Despite the increasing evidence on the adverse effects of heavy metals, the detailed mechanisms underlying their action on lung cancer progression are still poorly understood. In the present study, we investigated whether heavy metals (mercury chloride and lead acetate) affect cell viability, cell cycle, and apoptotic cell death in human lung fibroblast MRC5 cells. The results showed that mercury chloride arrest

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2

Siczkowski, M., J. E. Davies, and L. L. Ng. "Activity and density of the Na+/H+ antiporter in normal and transformed human lymphocytes and fibroblasts." American Journal of Physiology-Cell Physiology 267, no. 3 (1994): C745—C752. http://dx.doi.org/10.1152/ajpcell.1994.267.3.c745.

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The turnover number for the sodium-hydrogen exchanger isoform 1 (NHE-1) has been determined in human lymphocytes and MRC5 fibroblasts and in their virally transformed counterparts. Using fluorometric methods, we have determined the intracellular pH and Na+/H+ antiport activity of these cells. Intracellular pH was elevated in both lines of transformed cells. In contrast, Na+/H+ antiport activity was apparently unchanged in simian virus 40-transformed MRC5 fibroblasts (MRC5 SV1 TV1 28.9 +/- 5.2 mM/min compared with MRC5 fibroblasts 26.5 +/- 5.3 mM/min) but slightly increased in Epstein-Barr viru

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3

Mandron, Marie, Hélène Martin, Béatrice Bonjean, Jacqueline Lulé, Eric Tartour, and Christian Davrinche. "Dendritic cell-induced apoptosis of human cytomegalovirus-infected fibroblasts promotes cross-presentation of pp65 to CD8+ T cells." Journal of General Virology 89, no. 1 (2008): 78–86. http://dx.doi.org/10.1099/vir.0.83278-0.

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An efficient host response to human cytomegalovirus (HCMV) infection may depend on rapid sensing of the infection by the innate immune response prior to deployment of viral immunosubversive functions. Control of HCMV dissemination could be ensured by apoptosis of cells immediately following infection. In the present report, it is demonstrated that changes in the ratio of c-FLIP to FLICE contributed to early sensitivity of HCMV-infected MRC5 fibroblasts to tumour necrosis factor alpha (TNF-α), providing an innate response to infection. Dendritic cells (DCs) co-cultured with HCMV-infected MRC5 c

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Peristeris, Platon, Alexandre Gaspar, Pascal Gros, Philippe Laurent, Hélène Bernon, and Jacques Bienvenu. "Effects of serum amyloid A protein on lymphocytes, HeLa, and MRC5 cells in culture." Biochemistry and Cell Biology 67, no. 7 (1989): 365–70. http://dx.doi.org/10.1139/o89-057.

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A major human acute phase protein, the serum amyloid A protein, has been tested in vitro for its effect on lymphocyte proliferation, the formation of E-stable rosettes, as well as the growth of HeLa and MRC5 cell cultures. Serum amyloid A protein has been found to be markedly inhibitory at 30, 100, 200, and 300 μg/mL, and is a very potent inhibitor of in vitro biological functions.Key words: serum amyloid A protein, lymphocytes, HeLa cells, MRC5 cells.

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5

Giatromanolaki, Alexandra, Maria Liousia, Stella Arelaki, et al. "Differential effect of hypoxia and acidity on lung cancer cell and fibroblast metabolism." Biochemistry and Cell Biology 95, no. 3 (2017): 428–36. http://dx.doi.org/10.1139/bcb-2016-0197.

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This study examined the metabolic response of lung cancer cells and normal lung fibroblasts to hypoxia and acidity. GLUT1 and HXKII mRNA/protein expression was up-regulated under hypoxia in the MRC5 fibroblasts and in the A549 and H1299 lung cancer cell lines, indicating intensified glucose absorption and glycolysis. Under hypoxia, the LDHA mRNA and LDH5 protein levels increased in the cancer cells but not in the fibroblasts. Acidity suppressed the above-mentioned hypoxia effect. PDH-kinase-1 (PDK1 mRNA and protein) and inactive phosphorylated-PDH protein levels were induced under hypoxia in t

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Ng-Blichfeldt, John-Poul, Tristan de Jong, Rosa K. Kortekaas та ін. "TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation". American Journal of Physiology-Lung Cellular and Molecular Physiology 317, № 1 (2019): L14—L28. http://dx.doi.org/10.1152/ajplung.00400.2018.

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Transforming growth factor-β (TGF-β)-induced fibroblast-to-myofibroblast differentiation contributes to remodeling in chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis, but whether this impacts the ability of fibroblasts to support lung epithelial repair remains little explored. We pretreated human lung fibroblasts [primary (phFB) or MRC5 cells] with recombinant human TGF-β to induce myofibroblast differentiation, then cocultured them with adult mouse lung epithelial cell adhesion molecule-positive cells (EpCAM+) to investigate their capacity to support epithelial organoi

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7

Dai, Yu-heng, Xiao-qing Li, Da-peng Dong, Hai-bo Gu, Cheng-ying Kong та Zhihao Xu. "P27 Promotes TGF-β-Mediated Pulmonary Fibrosis via Interacting with MTORC2". Canadian Respiratory Journal 2019 (19 вересня 2019): 1–9. http://dx.doi.org/10.1155/2019/7157861.

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Pulmonary fibrosis (PF), a progressive and life-threatening pulmonary disease, is the main pathological basis of interstitial lung disease (ILD) which includes the idiopathic pulmonary fibrosis (IPF). No effective therapeutic strategy for pulmonary fibrosis has been established. TGF-β signaling has emerged as the vital regulator of PF; however, the detailed molecular mechanisms of TGF-β in PF were uncertain. In the present study, we proved that inhibition of MTORC2 suppresses the expression of P27 in MRC5 and HLF cells. And in bleomycin-induced PF model, the expression of α-SMA and P27 was upr

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8

Abdalla, Ashraf N., Usama Shaheen, Qasem M. A. Abdallah, et al. "Proapoptotic Activity of Achillea membranacea Essential Oil and Its Major Constituent 1,8-Cineole against A2780 Ovarian Cancer Cells." Molecules 25, no. 7 (2020): 1582. http://dx.doi.org/10.3390/molecules25071582.

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Among the hundreds of reported Achillea species, A. membranacea (Labill.) DC. is one of the six that grow in Jordan. Many species of this genus are used in folk medicine to treat a variety of ailments and several biological and pharmacological activities have been ascribed to their essential oil (EO). For this study, the EO obtained from a specimen of A. membranacea grown in Jordan was analyzed by GC-MS. Ninety-six compounds were detected, of which oxygenated monoterpenes was the predominant class (47.9%), followed by non-terpene derivatives (27.9%), while sesquiterpenes represented 14.2% of t

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9

Koulaouzidou, Elisabeth A., Maria Helvatjoglu-Antoniades, George Palaghias, Artemis Karanika-Kouma, and Dimitrios Antoniades. "Cytotoxicity of Dental Adhesives In Vitro." European Journal of Dentistry 03, no. 01 (2009): 03–09. http://dx.doi.org/10.1055/s-0039-1697399.

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ABSTRACTObjectives: The purpose of this study was to evaluate the cytotoxic effect of six dental adhesives (Admira Bond, Clearfil Liner Bond 2V, ED Primer II, Fuji Bond LC, Gluma Comfort Bond, and NanoBond) applied to cell cultures.Methods: The experiments were performed on two cell lines, rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC5). Samples of the adhesives were light-cured and placed in culture medium for 24 hours. The extraction media was applied on the RPC-C2A and the MRC5 cells. Complete medium was used as a control. Cytotoxicity was evaluated with a modified sulforhodamine

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10

Boulos, Areen, Jean-Marc Rolain, and Didier Raoult. "Antibiotic Susceptibility of Tropheryma whipplei in MRC5 Cells." Antimicrobial Agents and Chemotherapy 48, no. 3 (2004): 747–52. http://dx.doi.org/10.1128/aac.48.3.747-752.2004.

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ABSTRACT Whipple's disease is considered a rare chronic disease with a broad spectrum of clinical manifestations. Several antibiotics have been used for the treatment of this disease, and the current reference treatment was determined empirically on the basis of only a few clinical observations. Patients should be treated for months, and many relapse after antibiotic withdrawal. We report here the first extensive study on the susceptibilities of three reference strains of Tropheryma whipplei to antibiotic in cell culture by using a real-time PCR assay as previously described. We found that dox

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11

Sairi, Anith M. M., Siti Izera Ismail, Arina Sukor, et al. "Cytotoxicity and Anticancer Activity of Donkioporiella mellea on MRC5 (Normal Human Lung) and A549 (Human Lung Carcinoma) Cells Lines." Evidence-Based Complementary and Alternative Medicine 2020 (December 9, 2020): 1–10. http://dx.doi.org/10.1155/2020/7415672.

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Polypores are mushrooms which are rich in bioactivities and for generations, they have been widely used as herbal remedies. Despite their significant importance in treatments of various health issues, only a few local species have been reported for their pharmacological potentials. The present study was carried out to establish cytotoxicity potentials of Donkioporiella mellea, a local polypore species collected from forested areas in Malaysia at cellular levels on normal human lung (MRC5) and human lung carcinoma (A549) cell lines. Survival and inhibition rates were analyzed by 3-(4, 5)-dimeth

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12

Hong, Sok-Lai, Guan-Serm Lee, Syarifah Nur Syed Abdul Rahman, et al. "Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines." Scientific World Journal 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/397430.

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Curcuma purpurascensBl., belonging to the Zingiberaceae family, is known astemu tisin Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil oftemu tisshowed turmerone as the major component, followed by germacrone,ar-turmerone, germacrene-B, and curlone. The rhizome oil oftemu tisexhibited strong cytotox

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13

Wong, Yau Hsiung, Habsah Abdul Kadir, and Sui Kiong Ling. "Bioassay-Guided Isolation of Cytotoxic Cycloartane Triterpenoid Glycosides from the Traditionally Used Medicinal PlantLeea indica." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–11. http://dx.doi.org/10.1155/2012/164689.

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Leea indicais a medicinal plant used traditionally to cure cancer. In this study, the cytotoxic compounds ofL. indicawere isolated using bioassay-guided approach. Two cycloartane triterpenoid glycosides, mollic acid arabinoside (MAA) and mollic acid xyloside (MAX), were firstly isolated fromL. indica. They inhibited the growth of Ca Ski cervical cancer cells with IC50of 19.21 μM (MAA) and 33.33 μM (MAX). MRC5 normal cell line was used to calculate selectivity index. MAA and MAX were about 8 and 4 times more cytotoxic to Ca Ski cells compared to MRC5. The cytotoxicity of MAA was characterized b

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14

RAIMONDI, L., G. BANCHELLI, D. DALMAZZI, et al. "Sedum telephium L.Polysaccharide Content Affects MRC5 Cell Adhesion to Laminin and Fibronectin." Journal of Pharmacy and Pharmacology 52, no. 5 (2000): 585–91. http://dx.doi.org/10.1211/0022357001774237.

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15

Hadjur, C., M. J. Richard, M. O. Parat, A. Favier, and P. Jardon. "Photodynamically induced cytotoxicity of hypericin dye on human fibroblast cell line MRC5." Journal of Photochemistry and Photobiology B: Biology 27, no. 2 (1995): 139–46. http://dx.doi.org/10.1016/1011-1344(94)07064-u.

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16

Djan, I., B. Petrovic, S. Solajic, M. Djan, G. Bogdanovic, and M. Erak. "EP-1584 LOW DOSE PRE-IRRADIATION EFFECT ON CELL SURVIVAL IN HT29 AND MRC5 CELL LINES." Radiotherapy and Oncology 103 (May 2012): S607—S608. http://dx.doi.org/10.1016/s0167-8140(12)71917-5.

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17

Perrigue, Patrick M., Magdalena Rakoczy, Kamila P. Pawlicka, et al. "Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts." Cancers 12, no. 7 (2020): 1745. http://dx.doi.org/10.3390/cancers12071745.

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Cellular senescence is a tumor-suppressive mechanism blocking cell proliferation in response to stress. However, recent evidence suggests that senescent tumor cells can re-enter the cell cycle to become cancer stem cells, leading to relapse after cancer chemotherapy treatment. Understanding how the senescence reprogramming process is a precursor to cancer stem cell formation is of great medical importance. To study the interplay between senescence, stemness, and cancer, we applied a stem cell medium (SCM) to human embryonic fibroblasts (MRC5 and WI-38) and cancer cell lines (A549 and 293T). MR

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18

Couzinet, Sabine, Elisabeth Cejas, Johannes Schittny, Peter Deplazes, Rainer Weber, and Stefan Zimmerli. "Phagocytic Uptake of Encephalitozoon cuniculi by Nonprofessional Phagocytes." Infection and Immunity 68, no. 12 (2000): 6939–45. http://dx.doi.org/10.1128/iai.68.12.6939-6945.2000.

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ABSTRACT Encephalitozoon cuniculi is an obligate intracellular, spore-forming parasite belonging to the microsporidia that can cause disseminated infection in immunocompromised persons. E. cuniculi spores infect host cells by germination, i.e., by explosively everting the polar filament, through which the spore contents (sporoplasms) are subsequently injected into the cytoplasm. In addition, we observed intracellular, nongerminated spores in various nonprofessional phagocytes. In MRC5 cells, the number of internalized spores was approximately 10-fold higher than the number of injected sporopla

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Wang, Qiuli, Muwen Yang, Ye Zhang, Li Zhong, and Xinyu Zheng. "Novel Combination Oncolytic Adenoviral Gene Therapy Armed with Dm-dNK and CD40L for Breast Cancer." Current Gene Therapy 19, no. 1 (2019): 54–65. http://dx.doi.org/10.2174/1566523219666190307094713.

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Background:Both Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) suicide gene therapy and exogenous CD40 ligand (CD40L)-CD40 interaction in cancer via conditionally replicating adenovirus can selectively kill tumors without damaging normal tissues.Objective:To further improve the cancer killing effect, we investigated the therapeutic effect of combined cancer gene therapy based on a selective oncolytic adenovirus vector containing Dm-dNK suicide gene and exogenous CD40L on breast carcinoma cells in vitro and in vivo.Methods:A series of conditionally replicating adenoviruses using ad

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20

Rezig, Dorra, Henda Touzi, Zina Meddeb, and Henda Triki. "Cytopathic effect of Human cosavirus (HCoSV) on primary cell cultures of human embryonic lung MRC5." Journal of Virological Methods 207 (October 2014): 12–15. http://dx.doi.org/10.1016/j.jviromet.2014.06.011.

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21

Won, Sungyong, Tetsuro Ikegami, C. J. Peters, and Shinji Makino. "NSm and 78-Kilodalton Proteins of Rift Valley Fever Virus Are Nonessential for Viral Replication in Cell Culture." Journal of Virology 80, no. 16 (2006): 8274–78. http://dx.doi.org/10.1128/jvi.00476-06.

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ABSTRACT Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental vir

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Yuan, Yingli, Luguo Sun, Xu Wang, et al. "Identification of a new GLDC gene alternative splicing variant and its protumorigenic roles in lung cancer." Future Oncology 15, no. 36 (2019): 4127–39. http://dx.doi.org/10.2217/fon-2019-0403.

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Aim: To clarify the regulatory roles of GLDCV1, the first identified truncated glycine decarboxylase (GLDC), on cancer stem cells and tumorigenesis. Materials & methods: RT-PCR or RT-qPCR, immunoblotting and immunohistochemical staining were applied to assess gene expression. MTT, BrdU incorporation and colony formation assays were used to examine cell proliferation capacity. Soft agar colony formation and in vivo transplantation were applied to evaluate cellular transformation and tumorigenesis. Results & conclusion: Expression of GLDCV1 or GLDC was enhanced in non-small-cell lung can

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Knight, Andrea, Stephen Mackinnon та Mark W. Lowdell. "CMV-Reactive γ/δ T Cells Are Equivalent to Their α/β Counterparts In the Lysis of CMV-Infected Targets – Potential for Post Transplant Adoptive Immunotherapy." Blood 116, № 21 (2010): 1277. http://dx.doi.org/10.1182/blood.v116.21.1277.1277.

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Abstract Abstract 1277 Reactivation of cytomegalovirus (CMV) post allogeneic stem cell transplantation (SCT) can be prevented and treated by adoptive immunotherapy with donor-derived alpha/beta CMV-reactive T cells (CMV-T). We have reported that VD1+ gamma/delta T cells arising post allogeneic SCT are also CMV-reactive and that these cells can be identified in and isolated from CMV-seronegative donors (Knight et al 2010). The relative ability of VD1 gamma/delta cells to lyse CMV-infected targets compared to CMV-reactive alpha/beta T cells has not been determined. Here we have isolated gamma/de

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Arrode, Géraldine, Claire Boccaccio, Jacqueline Lulé, et al. "Incoming Human Cytomegalovirus pp65 (UL83) Contained in Apoptotic Infected Fibroblasts Is Cross-Presented to CD8+ T Cells by Dendritic Cells." Journal of Virology 74, no. 21 (2000): 10018–24. http://dx.doi.org/10.1128/jvi.74.21.10018-10024.2000.

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ABSTRACT Human cytomegalovirus (HCMV) infection is well controlled mainly by cytotoxic CD8+ T lymphocytes (CTL) directed against the matrix protein pp65 despite the numerous immune escape mechanisms developed by the virus. Dendritic cells (DCs) are key antigen-presenting cells for the generation of an immune response which have the capacity to acquire antigens via endocytosis of apoptotic cells and thus present peptides to major histocompatibility complex class I-restricted T cells. We examined whether this mechanism could contribute to the activation of anti-pp65 CTL. In this study, we show t

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Kumar, Brajesh, and Abhishek Suman. "Synthesis, spectroscopic characterization and biological application of copper complex of 5-carbethoxy-2-thiouracil." Journal of Drug Delivery and Therapeutics 10, no. 6 (2020): 145–48. http://dx.doi.org/10.22270/jddt.v10i6.4417.

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5-carbethoxy-2-thiouracil (eitotH2) reacts with CuX (X= Cl, Br, I) halides to give the formula [CuX(eitotH)2]2 dinuclear complexes, while the formula [CuX(PPh3)2(eitotH)2] mononuclear mixed ligand complexes result when reaction is carried out in the presence of two equivalent of triphenylphosphine (PPh3). The new copper (I) complexes were studied against two tumor cell lines, A549 (human pulmonary carcinoma cell line) and HeLa (human epithelial carcinoma cell line) and one regular immortalized cell line, MRC5 (human fetal lung fibroblast). In comparison to the phosphine free ones that hindered

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Lee, Dong Un, Min Jeong Ji, Jung Yun Kang, Sun Young Kyung, and Jeong Hee Hong. "Dust particles-induced intracellular Ca2+ signaling and reactive oxygen species in lung fibroblast cell line MRC5." Korean Journal of Physiology & Pharmacology 21, no. 3 (2017): 327. http://dx.doi.org/10.4196/kjpp.2017.21.3.327.

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Wang, Tien-Chun, Chih-Nan Fang, Chih-Chien Shen, et al. "Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–12. http://dx.doi.org/10.1155/2012/276032.

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Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassia

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Bader, Ammar, Majdi M. Bkhaitan, Ashraf N. Abdalla, et al. "Design and Synthesis of 4-O-Podophyllotoxin Sulfamate Derivatives as Potential Cytotoxic Agents." Evidence-Based Complementary and Alternative Medicine 2021 (January 25, 2021): 1–7. http://dx.doi.org/10.1155/2021/6672807.

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4-O-Podophyllotoxin sulfamate derivatives were prepared using the natural lignan podophyllotoxin. The prepared compounds were afforded by reacting O-sulfonyl chloride podophyllotoxin with ammonia or aminoaryl/heteroaryl motif. Biological evaluation was performed in human breast cancer (MCF7), ovarian cancer (A2780), colon adenocarcinoma (HT29), and normal lung fibroblast (MRC5) cell lines. Compound 3 exhibited potent inhibitory activity and good selectivity margin. Compounds 2, 3, and 7 exerted apoptotic effect in MCF7 cells in a dose-dependent manner. The cytotoxicity of the verified compound

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Vaisertrager, I. S. R., O. I. Podgornaya, and N. I. Enukashvily. "Constitutive heterochromatin DNA fragments are demethylated and decondensed in senescent primary fibroblasts MRC5 and malignant A431 cell line." Cell and Tissue Biology 1, no. 1 (2007): 50–57. http://dx.doi.org/10.1134/s1990519x07010075.

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Arrode, Géraldine, Claire Boccaccio, Jean-Pierre Abastado, and Christian Davrinche. "Cross-Presentation of Human Cytomegalovirus pp65 (UL83) to CD8+ T Cells Is Regulated by Virus-Induced, Soluble-Mediator-Dependent Maturation of Dendritic Cells." Journal of Virology 76, no. 1 (2002): 142–50. http://dx.doi.org/10.1128/jvi.76.1.142-150.2002.

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ABSTRACT Cytotoxic CD8+ T lymphocytes (CTL) directed against the matrix protein pp65 are major effectors in controlling infection against human cytomegalovirus (HCMV), a persistent virus of the Betaherpesvirus family. We previously suggested that cross-presentation of pp65 by nonpermissive dendritic cells (DCs) could overcome viral strategies that interfere with activation of CTL (G. Arrode, C. Boccaccio, J. Lule, S. Allart, N. Moinard, J. Abastado, A. Alam, and C. Davrinche, J. Virol. 74:10018–10024, 2000). It is well established that mature DCs are very potent in initiating T-cell-mediated i

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Hałas-Wiśniewska *, Marta, Wioletta Zielińska*, Karolina Matulewicz, et al. "The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549." Medical Research Journal 2, no. 3 (2018): 102–10. http://dx.doi.org/10.5603/mrj.2017.0013.

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Mohamad Saimi, Norfatin Izzatie, Norazlinaliza Salim, Noraini Ahmad, Emilia Abdulmalek, and Mohd Basyaruddin Abdul Rahman. "Aerosolized Niosome Formulation Containing Gemcitabine and Cisplatin for Lung Cancer Treatment: Optimization, Characterization and In Vitro Evaluation." Pharmaceutics 13, no. 1 (2021): 59. http://dx.doi.org/10.3390/pharmaceutics13010059.

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Gemcitabine (Gem) and cisplatin (Cis) are currently being used for lung cancer treatment, but they are highly toxic in high dosages. This research aimed to develop a niosome formulation containing a low-dosage Gem and Cis (NGC), as an alternative formulation for lung cancer treatment. NGC was prepared using a very simple heating method and was further optimized by D-optimal mixture design. The optimum NGC formulation with particle size, polydispersity index (PDI), and zeta potential of 166.45 nm, 0.16, and −15.28 mV, respectively, was obtained and remained stable at 27 °C with no phase separat

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Khalid, Asaad, Hassan A. Alhazmi, Ashraf N. Abdalla, et al. "GC-MS Analysis and Cytotoxicity Evaluation of Shammah (Smokeless Tobacco) Samples of Jazan Region of Saudi Arabia as Promoter of Cancer Cell Proliferation." Journal of Chemistry 2019 (April 15, 2019): 1–8. http://dx.doi.org/10.1155/2019/3254836.

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Shammah is a locally manufactured form of smokeless tobacco (ST) which is traditionally used in Middle Eastern countries including Saudi Arabia, Sudan, and Yemen. Presence of a high concentration of nicotine, in addition to various other toxic and carcinogenic constituents, makes it a serious human health threat. It is an admixture containing powdered tobacco, along with several additives, such as lime, ash, black pepper, volatile oils, and flavoring agents. This study was conducted to investigate the constituents of eight different samples of widely used shammah varieties in the Jazan region

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Davies, J. E., and L. L. Ng. "Simvastatin and Intracellular pH Regulation by the Na+/H+ Antiport of Sv40-Virus-Transformed Human Mrc5 Fibroblasts." Clinical Science 84, no. 6 (1993): 633–43. http://dx.doi.org/10.1042/cs0840633.

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1. Inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase by simvastatin leads to inhibition of both cell growth and Na+/H+ antiport activity. The effect of simvastatin on intracellular pH and Na+/H+ antiport activity was therefore studied on an adherent cell line, the SV40-virus-transformed MRC5 human fibroblast. 2. Simvastatin led to a dose-dependent decrease in intracellular pH, attributed to a reduction in Na+/H+ exchange, together with a rounding of cell shape. Mevalonate (1 mmol/l) prevented these effects of simvastatin, and when added after inhibition of the antiport by simvastatin, rev

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Saklatvala, J., P. Kaur, and F. Guesdon. "Phosphorylation of the small heat-shock protein is regulated by interleukin 1, tumour necrosis factor, growth factors, bradykinin and ATP." Biochemical Journal 277, no. 3 (1991): 635–42. http://dx.doi.org/10.1042/bj2770635.

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Interleukin 1 (IL1) increased phosphorylation of the small heat-shock protein (hsp 27) in MRC5 fibroblasts. The increase was maintained for at least 30 min, but levels had returned to pre-stimulation values by 2 h. When hsp 27 was metabolically labelled with [3H]leucine, about 15% was phosphorylated in resting confluent cells; this rose to 90% upon stimulation by IL1. Peptide maps of the three differently charged phosphorylated forms were consistent with their arising by phosphorylation of increasing numbers of serine residues. IL1 had the same effect on hsp 27 in pig articular chondrocytes, e

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Mazière, Cécile, Arnaud Meignotte, Françoise Dantin, Marie-Alix Conte, and Jean-Claude Mazière. "Oxidized LDL Induces an Oxidative Stress and Activates the Tumor Suppressor p53 in MRC5 Human Fibroblasts." Biochemical and Biophysical Research Communications 276, no. 2 (2000): 718–23. http://dx.doi.org/10.1006/bbrc.2000.3528.

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Wharton, S. A., and A. R. Hipkiss. "Degradation of peptides and proteins of different sizes by homogenates of human MRC5 lung fibroblasts." FEBS Letters 184, no. 2 (1985): 249–53. http://dx.doi.org/10.1016/0014-5793(85)80616-5.

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Manríquez-Torres, José de Jesús, Marco Antonio Hernández-Lepe, José Román Chávez-Méndez, et al. "Isolation and Cytotoxic Activity of Phyllocladanes from the Roots of Acacia schaffneri (Leguminosae)." Molecules 25, no. 17 (2020): 3944. http://dx.doi.org/10.3390/molecules25173944.

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In research on natural molecules with cytotoxic activity that can be used for the development of new anticancer agents, the cytotoxic activity of hexane, chloroform, and methanol extracts from the roots of Acacia schaffneri against colon, lung, and skin cancer cell lines was explored. The hexane extract showed the best activity with an average IC50 of 10.6 µg mL−1. From this extract, three diterpenoids, phyllocladan-16α,19-diol (1), phyllocladan-16α-ol (2), and phylloclad-16-en-3-ol (3), were isolated and characterized by their physical and spectroscopic properties. Diterpenoids 1 and 2 were t

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Willett, Mark, Michele Brocard, Alexandre Davide, and Simon J. Morley. "Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts." Biochemical Journal 438, no. 1 (2011): 217–27. http://dx.doi.org/10.1042/bj20110435.

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Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scannin

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40

Haferlach, Claudia, Torsten Haferlach, Tamara Alpermann, Wolfgang Kern, and Susanne Schnittger. "Specific Molecular Mutations with Prognostic Relevance in AML with Normal Karyotype Are Also Associated with Outcome in AML with Aberrant Karyotype: A Study on 1981 Cases,." Blood 118, no. 21 (2011): 3517. http://dx.doi.org/10.1182/blood.v118.21.3517.3517.

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Abstract Abstract 3517 Background and Aim: The karyotype and molecular mutations are well established prognostic parameters in AML. However, the impact on outcome of molecular mutations has been evaluated mainly in the subset of AML with normal karyotype (NK). The aim of this study was 1. to determine the frequency of NPM1 mutations (NPM1 mut), partial tandem duplications within the MLL gene (MLL -PTD), length mutations within the FLT3 gene (FLT3 -ITD) and CEPBA mutations (CEPBA mut) in distinct cytogenetic subgroups and 2. to evaluate the prognostic impact of these mutations in relation to ch

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Abubakar, Ibrahim Babangida, та Hwei-San Loh. "Potentiation of in vitro apoptotic effects of δ-Tocotrienol and Jerantinine A on human lung adenocarcinoma cells". Journal of Herbmed Pharmacology 8, № 4 (2019): 333–38. http://dx.doi.org/10.15171/jhp.2019.49.

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Introduction: The apoptotic effects of single-compound and combined sub-effective concentrations of δ-tocotrienol and jerantinine A on human lung adenocarcinoma (A549) cells were investigated. Methods: Assays including cell viability, histochemical and immunofluorescence staining techniques, flow cytometry and enzyme activity were used. Results: The combination of δ-tocotrienol with jerantinine A at sub-effective concentrations induced a synergistic effect and improved selective toxicity towards cancerous A549 cells over normal lung fibroblast (MRC5) cells compared to the single-compound jeran

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Gao, Xue, Yuming Zhou, Xiaoqi Zheng, et al. "Peniciketal A, A Novel Spiroketal Compound, Exerts Anticancer Effects by Inhibiting Cell Proliferation, Migration and Invasion of A549 Lung Cancer Cells." Anti-Cancer Agents in Medicinal Chemistry 18, no. 11 (2019): 1573–81. http://dx.doi.org/10.2174/1871520618666180604090802.

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Background: Peniciketal A (Pe-A) is a spiroketal compound isolated from saline soil-derived fungus Penicillium raistrickii. However, its role for biological processes has not been clarified. In this study, we for the first time investigated the anticancer effects and the underlying mechanisms of Pe-A in A549 lung cancer cells. Metheds: Cell proliferation was tested by MTT assay and colony formation assay. Flow cytometry was performed to examine the cell cycle, apoptosis and mitochondrial membrane potential. Invasion and migration were analyzed using transwell assay and wound healing analysis.

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Ballerini, Paola, Pierre-Yves Boelle, Caroline Deswarte, et al. "Prognostic Significance of SALL4 Expression Levels in Paediatric Acute Myeloid Leukaemia (AML)." Blood 112, no. 11 (2008): 2243. http://dx.doi.org/10.1182/blood.v112.11.2243.2243.

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Abstract Pediatric AML still represent an unfavourable disease resulting from the heterogeneous clonal expansion of malignant transformed haematopoietic stem or progenitor cell. The leukemia cell population is continuously replenish by rare, functionally distinct “leukaemia stem cells” (LSC) endowed with the capacity to self renew as well with the ability to generate clonogenic leukemic progenitors The AML-LSCs have been well documented and seem to behave like quiescent or slowly dividing hematopoietic stem cells. Therefore, LSC are considered less sensitive to treatments, which rather target

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Abdalla, Ashraf N., Waleed H. Malki, Amal Qattan, Imran Shahid, Mohammad Akbar Hossain, and Muhammad Ahmed. "Chemosensitization of HT29 and HT29-5FU Cell Lines by a Combination of a Multi-Tyrosine Kinase Inhibitor and 5FU Downregulates ABCC1 and Inhibits PIK3CA in Light of Their Importance in Saudi Colorectal Cancer." Molecules 26, no. 2 (2021): 334. http://dx.doi.org/10.3390/molecules26020334.

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Colorectal cancer (CRC) remains one of the main causes of death worldwide and in Saudi Arabia. The toxicity and the development of resistance against 5 fluorouracil 5FU pose increasing therapeutic difficulties, which necessitates the development of personalized drugs and drug combinations. Objectives: First, to determine the most important kinases and kinase pathways, and the amount of ABC transporters and KRAS in samples taken from Saudi CRC patients. Second, to investigate the chemosensitizing effect of LY294002 and HAA2020 and their combinations with 5FU on HT29, HT29-5FU, HCT116, and HCT11

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Abdalla, Ashraf N., Waleed H. Malki, Amal Qattan, Imran Shahid, Mohammad Akbar Hossain, and Muhammad Ahmed. "Chemosensitization of HT29 and HT29-5FU Cell Lines by a Combination of a Multi-Tyrosine Kinase Inhibitor and 5FU Downregulates ABCC1 and Inhibits PIK3CA in Light of Their Importance in Saudi Colorectal Cancer." Molecules 26, no. 2 (2021): 334. http://dx.doi.org/10.3390/molecules26020334.

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Colorectal cancer (CRC) remains one of the main causes of death worldwide and in Saudi Arabia. The toxicity and the development of resistance against 5 fluorouracil 5FU pose increasing therapeutic difficulties, which necessitates the development of personalized drugs and drug combinations. Objectives: First, to determine the most important kinases and kinase pathways, and the amount of ABC transporters and KRAS in samples taken from Saudi CRC patients. Second, to investigate the chemosensitizing effect of LY294002 and HAA2020 and their combinations with 5FU on HT29, HT29-5FU, HCT116, and HCT11

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Kundaković, Tatjana, Tatjana Stanojković, Branka Kolundžija, et al. "Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp. pisidica (Lamiceae)." Natural Product Communications 9, no. 4 (2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900437.

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The antimicrobial and cytotoxic activities of the essential oil of Satureja montana ssp. pisidica from two localities (mountains Korab and Galičica) were studied. Forty-nine components were identified in the each sample. Oxygenated monoterpene hydrocarbons were the major compounds: carvacrol, thymol, carvacrol methyl ether and β-linalool. Both tested essential oils showed very high and similar antimicrobial activity. Minimal inhibitory concentrations ranged from 12.5 μg/mL against S. epidermidis to 50 μg/mL against P. aeruginosa and C. albicans. The cytotoxic effect of the essential oils was t

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Willett, Mark, Michele Brocard, Hilary J. Pollard, and Simon J. Morley. "mRNA encoding WAVE–Arp2/3-associated proteins is co-localized with foci of active protein synthesis at the leading edge of MRC5 fibroblasts during cell migration." Biochemical Journal 452, no. 1 (2013): 45–55. http://dx.doi.org/10.1042/bj20121803.

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During cell spreading, mammalian cells migrate using lamellipodia formed from a large dense branched actin network which produces the protrusive force required for leading edge advancement. The formation of lamellipodia is a dynamic process and is dependent on a variety of protein cofactors that mediate their local regulation, structural characteristics and dynamics. In the present study, we show that mRNAs encoding some structural and regulatory components of the WAVE [WASP (Wiskott–Aldrich syndrome protein) verprolin homologous] complex are localized to the leading edge of the cell and assoc

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Sable, J., A. Amiglir, C. Daniels, N. Hazi, and A. Laskowski. "Oncolytic virus production using MRC5 cells in Pall's iCELLis nano bioreactor is equivalent in high and low compaction beds." Cytotherapy 22, no. 5 (2020): S150—S151. http://dx.doi.org/10.1016/j.jcyt.2020.03.310.

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Taylor, Lisa M., Alexander James, Christine E. Schuller, Jesena Brce, Richard B. Lock, and Karen L. MacKenzie. "Inactivation of p16INK4a, with Retention of pRB and p53/p21cip1 Function, in Human MRC5 Fibroblasts That Overcome a Telomere-independent Crisis during Immortalization." Journal of Biological Chemistry 279, no. 42 (2004): 43634–45. http://dx.doi.org/10.1074/jbc.m402388200.

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Singh-Kaw, P., R. Zarnegar, and J. M. Siegfried. "Stimulatory effects of hepatocyte growth factor on normal and neoplastic human bronchial epithelial cells." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 6 (1995): L1012—L1020. http://dx.doi.org/10.1152/ajplung.1995.268.6.l1012.

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We examined the mitogenic, chemoinvasive, and chemotactic effects of hepatocyte growth factor (HGF) toward normal and neoplastic human epithelial cells derived from the bronchial mucosa. Primary cultures of human bronchial epithelial cells (HBE cells), immortalized bronchial epithelial cells (IB3-1 cells), and cells derived from a squamous cell carcinoma of the lung (128-88T cells) were used as targets. HGF was mitogenic for all three cell types as measured by bromodeoxyuridine (BrdU) labeling and colony-forming efficiency (CFE). With the use of BrdU labeling, 9.8-16.8% of nuclei were labeled

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