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Relevant bibliographies by topics / MRNA profiling

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Academic literature on the topic 'MRNA profiling'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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Journal articles on the topic "MRNA profiling"

1

Fortina, Paolo, and Saul Surrey. "Digital mRNA profiling." Nature Biotechnology 26, no. 3 (2008): 293–94. http://dx.doi.org/10.1038/nbt0308-293.

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Fisunov, G. Yu, D. V. Evsyutina, A. A. Arzamasov, I. O. Butenko, and V. M. Govorun. "Profiling of Mycoplasma gallisepticum Ribosomes." Acta Naturae 7, no. 4 (2015): 107–12. http://dx.doi.org/10.32607/20758251-2015-7-4-107-112.

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The development of high-throughput technologies is increasingly resulting in identification of numerous cases of low correlation between mRNA and the protein level in cells. These controversial observations were made on various bacteria, such as E. coli, Desulfovibrio vulgaris, and Lactococcus lactis. Thus, it is important to develop technologies, including high-throughput techniques, aimed at studying gene expression regulation at the level of translation. In the current study, we performed proteomic profiling of M. gallisepticum ribosomes and identified high abundant noncanonical proteins. We found that binding of mRNAs to ribosomes is mainly determined by two parameters: (1) abundance of mRNA itself and (2) complimentary interactions between the 3 end of 16S rRNA and the ribosome binding site in the 5-untranslated region of mRNA.

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Flintoft, Louisa. "mRNA profiling of activated neurons." Nature Reviews Genetics 14, no. 1 (2012): 4. http://dx.doi.org/10.1038/nrg3398.

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Vennemann, Marielle, and Antje Koppelkamm. "Postmortem mRNA profiling II: Practical considerations." Forensic Science International 203, no. 1-3 (2010): 76–82. http://dx.doi.org/10.1016/j.forsciint.2010.07.007.

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Haas, C., B. Klesser, A. Kratzer, and W. Bär. "mRNA profiling for body fluid identification." Forensic Science International: Genetics Supplement Series 1, no. 1 (2008): 37–38. http://dx.doi.org/10.1016/j.fsigss.2007.10.064.

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Fabbri, M., M. Venturi, A. Talarico, P. Frisoni, R. M. Gaudio, and M. Neri. "mRNA profiling in ancient blood stains." Forensic Science International: Genetics Supplement Series 6 (December 2017): e500-e503. http://dx.doi.org/10.1016/j.fsigss.2017.09.182.

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Li, Y., X. Zhou, M. A. R. St. John, and D. T. W. Wong. "RNA Profiling of Cell-free Saliva Using Microarray Technology." Journal of Dental Research 83, no. 3 (2004): 199–203. http://dx.doi.org/10.1177/154405910408300303.

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Saliva, like other bodily fluids, has been used to monitor human health and disease. This study tests the hypothesis that informative human mRNA exists in cell-free saliva. If present, salivary mRNA may provide potential biomarkers to identify populations and patients at high risk for oral and systemic diseases. Unstimulated saliva was collected from ten normal subjects. RNA was isolated from the cell-free saliva supernatant and linearly amplified. High-density oligonucleotide microarrays were used to profile salivary mRNA. The results demonstrated that there are thousands of human mRNAs in cell-free saliva. Quantitative PCR (Q-PCR) analysis confirmed the present of mRNA identified by our microarray study. A reference database was generated based on the mRNA profiles in normal saliva. Our finding proposes a novel clinical approach to salivary diagnostics, Salivary Transcriptome Diagnostics (STD), for potential applications in disease diagnostics as well as normal health surveillance.

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Wang, Ena, Lance D. Miller, Galen A. Ohnmacht, Edison T. Liu, and Francesco M. Marincola. "High-fidelity mRNA amplification for gene profiling." Nature Biotechnology 18, no. 4 (2000): 457–59. http://dx.doi.org/10.1038/74546.

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Fabbri, M., M. Venturi, A. Talarico, R. Inglese, R. M. Gaudio, and M. Neri. "mRNA profiling: Application to an old casework." Forensic Science International: Genetics Supplement Series 6 (December 2017): e380-e382. http://dx.doi.org/10.1016/j.fsigss.2017.09.170.

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Munchel, Sarah E., Ryan K. Shultzaberger, Naoki Takizawa, and Karsten Weis. "Dynamic profiling of mRNA turnover reveals gene-specific and system-wide regulation of mRNA decay." Molecular Biology of the Cell 22, no. 15 (2011): 2787–95. http://dx.doi.org/10.1091/mbc.e11-01-0028.

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RNA levels are determined by the rates of both transcription and decay, and a mechanistic understanding of the complex networks regulating gene expression requires methods that allow dynamic measurements of transcription and decay in living cells with minimal perturbation. Here, we describe a metabolic pulse-chase labeling protocol using 4-thiouracil combined with large-scale RNA sequencing to determine decay rates of all mRNAs in Saccharomyces cerevisiae. Profiling in various growth and stress conditions reveals that mRNA turnover is highly regulated both for specific groups of transcripts and at the system-wide level. For example, acute glucose starvation induces global mRNA stabilization but increases the degradation of all 132 detected ribosomal protein mRNAs. This effect is transient and can be mimicked by inhibiting the target-of-rapamycin kinase. Half-lives of mRNAs critical for galactose (GAL) metabolism are also highly sensitive to changes in carbon source. The fast reduction of GAL transcripts in glucose requires their dramatically enhanced turnover, highlighting the importance of mRNA decay in the control of gene expression. The approach described here provides a general platform for the global analysis of mRNA turnover and transcription and can be applied to dissect gene expression programs in a wide range of organisms and conditions.

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Dissertations / Theses on the topic "MRNA profiling"

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Mayer, Andreas. "Insights into the mRNA transcription cycle from genome-wide occupancy profiling." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-152649.

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Heimiller, Joseph Karl. "Genome-wide analysis of splicing requirements and function through mRNA profiling." Thesis, University of Colorado at Boulder, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3607314.

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<p> The RNA-binding proteins U2AF and PTB play important roles in gene expression in many eukaryotic species. Although U2AF and PTB have been well-studied, their functional requirements have not been investigated on a genome-wide scale. In this thesis, I analyze RNA expression data to determine the requirement of the general splicing factor U2AF in <i>S. pombe</i> and to identify genes misregulated in Drosophila PTB mutants. I find that many introns are insensitive to U2AF inactivation in a <i>Schizosaccharomyces pombe</i> U2AF59 mutant, <i>prp2.1.</i> Bioinformatics analysis indicates that U2AF-insensitive introns have stronger 5' splice sites and higher A/U composition. The importance of intronic nucleotide composition was further investigated using wild type RNA expression data sets. I show that nucleotide composition is a relatively important factor for regulated intron retention in a variety of species. I also analyzed the RNA-binding protein PTB using RNA Seq data to reveal genes misregulated in PTB mutants in <i>D. melanogaster.</i> I identify misregulation of alternative splicing in PTB mutants and putative PTB binding sites. In the PTB embryonic lethal mutant, which shows dorsoventral patterning defects, I show that dorsal fate genes are significantly up-regulated. I present a model to link PTB to dorsal closure defects. This thesis provides the first genome-wide analysis of U2AF in <i>S. pombe</i> and PTB in <i>Drosophila melanogaster. </i></p>

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Blin-Gonthier, Juliana. "Regulation of mRNA translation during the inflammatory response in murine macrophages." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSEN032.

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La régulation dynamique de la synthèse des protéines en fonction des besoins de la cellule facilite son adaptation face aux fluctuations de l’environnement. Malgré l’importance de la régulation de la traduction au cours du processus d’expression des gènes, l’impact de ce mécanisme sur des processus biologiques fondamentaux, comme la mise en place d’une réponse immunitaire, reste mal compris. Grâce au développement de nouvelles technologies basées sur l’utilisation du séquençage à haut débit, comme le ribosome profiling, il est désormais possible d’étudier en détails la façon dont la synthèse des protéines est contrôlée. Le monosome vs polysome footprinting est une nouvelle méthode qui permet d’étudier la traduction des ARN messagers (ARNm) selon leur association avec un seul ribosome (monosome) ou avec plusieurs ribosomes (polysomes). Au cours de ma thèse, j’ai identifié les paramètres essentiels pour la mise en place d’une expérience de monosome vs polysome footprinting donnant des résultats fiables en utilisant des macrophages primaires dérivés de la moëlle osseuse de souris. Je me suis intéressée à ce type de cellules immunitaires particulier car elles présentent une grande capacité à détecter des modifications dans leur environnement et à modifier leur taux d’expression de protéines en fonction des signaux reçus. Leur grande plasticité est notamment essentielle pour assurer leurs diverses fonctions de protection de l’organisme, comme le déclenchement et la résolution de la réponse inflammatoire. La méthode de monosome vs polysome footprinting ayant été initialement développée chez la levure, son utilisation avec un modèle d’étude différent a nécessité de nombreuses modifications du protocole. Suite à cette phase de développement technologique, j’ai pu confirmer que les monosomes, une population de ribosomes historiquement considérés comme inactifs, sont activement impliqués dans le processus de traduction dans les macrophages primaires de souris. Les données obtenues ont également permis d’identifier des caractéristiques communes entre les ARNm enrichis dans les monosomes chez la levure et dans les macrophages murins. La régulation de la synthèse des protéines via l’association à des monosomes ou à des polysomes pourrait donc être un mécanisme conservé chez les organismes eucaryotes. Enfin, le travail d’optimisation réalisé dans les macrophages primaires murins ouvre la possibilité d’étudier l’effet de la régulation de la traduction sur la mise en place et la résolution de la réponse inflammatoire de façon très détaillée<br>The dynamic regulation of the protein synthesis process participates in the cell adaptation to a constantly evolving environment. Despite its critical role in gene expression regulation, the understanding of translational control in fundamental biological processes, such as immune responses, is still incomplete. The implementation of new approaches based on deep sequencing can be used to fill the gap in the knowledge of protein synthesis regulation. Notably, monosome vs polysome footprinting is an innovative approach derived from ribosome profiling that allow the characterization of 80S footprints derived either from monosomes or polysomes associated ribosomes. In this work, I identified the key parameters required to obtain a robust picture of ribosomal densities across cellular mRNAs using monosome vs polysome footprinting in murine primary bone-marrow derived macrophages (pBMDM). These immune cells are particularly interesting to study protein synthesis regulation in evolving conditions as they display a high sensitivity towards their environment and have the ability to trigger different gene expression programs depending on external cues. Their high phenotypic plasticity is in fact essential to ensure their protective functions in the organism such as the triggering and the resolution of the inflammatory response. As monosome vs polysome footprinting was initially developed in yeast, the adaptation of this method to study murine immune cells required extensive optimizations. The resulting protocol developed in this work was used to confirm that, contrary to a long lasting belief in the scientific community, murine pBMDM monosomes are actively involved in the translation process. Interestingly, we were able to recapitulate similar observations to what was previously observed in yeast regarding the features of mRNAs preferentially bound to monosomes or polysomes in murine pBMDM. This could suggest that the differential trafficking of ribosomes depending on specific features of the cellular mRNAs is a conserved mechanism of translational control. Importantly, the distribution of ribosomes across the different mRNAs is not random and the proper ribosome allocation pattern could be critical to adapt protein synthesis levels to the cellular needs. Here we developed a robust strategy to study this overlooked transcript-specific mechanism of translational control. Moreover, our optimized protocol can now be used to study the impact of translation through monosomes or polysomes at different stages of the inflammatory response in murine macrophages

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Blasco, Moreno Bernat 1986. "Uncovering a novel function of the exonuclease Xrn1 in viral and cellular mRNA translation." Doctoral thesis, Universitat Pompeu Fabra, 2016. http://hdl.handle.net/10803/664207.

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The exonuclease Xrn1 degrades messenger RNAs (mRNAs) and controls transcription. By using a model system that replicates brome mosaic virus (BMV) in yeast, here we show that Xrn1 promotes translation initiation of BMV RNA. This function is linked to the highly structured 5’UTR and the coding sequence. The exonuclease activity of Xrn1 is required for its role in viral RNA translation. In contrast, the role of Xrn1 in translation is independent from its function in transcription. Importantly, ribosome profiling analyses reveal that Xrn1 acts as a translational activator of a specific group of cellular mRNAs enriched for glycosylation functions. As for BMV RNA, these cellular mRNAs depend on the exonuclease activity of Xrn1 for translational activation and contain long and structured 5’UTR. Our results reveal a new cross-talk between mRNA degradation and translation, and uncover an unexpected function of the exonuclease Xrn1 in viral and cellular translational control. Xrn1 és una exonucleasa que degrada l’ARN missatger (ARNm) i en regula la transcripció. Emprant un sistema model basat en el virus del mosaic del brom (BMV) i el llevat S. cerevisiae, en aquest treball es demostra que Xrn1 promou l’inici de la traducció de l’ARN viral. Aquesta funció en traducció està lligada a la regió no traduïda a l’extrem 5’ i a la seqüència codificant. L’activitat exonucleasa pròpia de Xrn1 és necessària per tal que actuï en traducció. En canvi, la funció de Xrn1 en transcripció és independent de la funció en traducció. Mitjançant estudis amb perfil de ribosomes, es va constatar que Xrn1 també actua com a activador de la traducció d’un subgrup d’ARNm cel·lulars. Aquests, codifiquen per funcions relacionades amb la glucosilació. Tal i com s’observa per l’ARN de BMV, aquests ARNm cel·lulars depenen de l’activitat exonucleasa de Xrn1 per a la seva traducció i tenen una elevada estructura secundària a la regió no traduïda de l’extrem 5’. En conjunt, els nostres resultats descriuen un nou exemple de la comunicació existent entre la degradació i la traducció de l’ARNm i revelen que Xrn1 té una funció inesperada en el control de la traducció de l’ARNm viral i cel·lular.<br>Xrn1 és una exonucleasa molt conservada entre espècies i que té un paper cabdal en la degradació de l’ARN missatger (ARNm) i la regulació de la transcripció. Emprant un sistema model basat en el virus del mosaic del brom (BMV) i el llevat Saccharomyces cerevisiae, en aquest treball es demostra que Xrn1 promou la traducció de l’ARN viral. Aquesta funció en traducció està lligada a la regió no traduïda a l’extrem 5’ i a la seqüència codiﬁcant. A més, mitjançant l’anàlisi amb perﬁls de polisomes s’observa que Xrn1 assisteix la iniciació de la traducció de l’ARN viral i que interacciona directament amb la maquinària de traducció. Tenint en compte que Xrn1 par􀀁cipa en la degradació i la transcripció d’ARNm, es van crear diferents mutants per tal d’analitzar la relació entre aquestes funcions i el rol de Xrn1 en traducció. En primer lloc, es va observar que l’ac􀀁vitat exonucleasa pròpia de Xrn1 és necessària per tal que Xrn1 actuï en traducció. Expressant la exonucleasa nuclear (Rat1) al citoplasma de cèl·lules xrn1∆, s’aconsegueix compensar els defectes en degradació de l’ARN i en la taxa de creixement. En canvi, no es rescaten els defectes en la traducció de l’ARN viral. En segon lloc, u􀀁litzant un mutant de Xrn1 que no pot ser importat al nucli (Xrn1∆NLS) es va concloure que la funció de Xrn1 en transcripció és independent de la funció en traducció. Tot seguit, mitjançant estudis amb perﬁl de ribosomes, es va constatar que Xrn1 també actua com a ac􀀁vador de la traducció d’un subgrup d’ARNm cel·lulars. Aquests, codiﬁquen per funcions relacionades amb la glucosilació i estan enriquits en proteïnes del re􀀁cle endoplasmà􀀁c. Tal i com s’observa per l’ARN de BMV, aquests ARNm cel·lulars depenen de l’ac􀀁vitat exonucleasa de Xrn1 per a la seva traducció i tenen una elevada estructura secundària a la regió no traduïda de l’extrem 5’. En conjunt, els nostres resultats descriuen un nou exemple de la comunicació existent entre la degradació i la traducció de l’ARNm i revelen que Xrn1 té una funció inesperada en el control de la traducció de l’ARNm viral i cel·lular.

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Hwang, Jae Yeon. "Analyses of mRNA Cleavage by RelE and the Role of tRNA Methyltransferase TrmD Using Bacterial Ribosome Profiling." BYU ScholarsArchive, 2016. https://scholarsarchive.byu.edu/etd/6443.

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Protein synthesis is a fundamental and ultimate process in living cells. Cells possess sophisticated machineries and continuously carry out complex processes. Monitoring protein synthesis in living cells not only inform us about the mechanism of translation but also deepen our insights about all aspects of life. Understanding the structure and mechanism of the ribosome and its associated factors helped us enlarge our knowledge on protein synthesis. Recently, with the dramatic advances of high-throughput sequencing and bioinformatics, a new technique called ribosome profiling emerged. By retrieving mRNA fragments protected by translating ribosomes, ribosome profiling reveals global ribosome occupancy along mRNAs in living cells, which can inform us with the identity and quantity of proteins being made. Easily adapted to other organisms, ribosome profiling technique is expanding its application in revealing various cellular activities as well as the knowledge on protein synthesis. Here, we report the mechanism of translating mRNA cleavage by endoribonuclease RelE in vivo. RelE is an endoribonuclease that is induced during nutrient deficiency stress and specifically cleaves translating mRNAs upon binding to the ribosomal A site. Overexpression of RelE in living cells causes growth arrest by inhibiting global translation. We monitored RelE activity in vivo upon overexpression using ribosome profiling. The data show that RelE actively cuts translating mRNAs whenever the ribosomal A site is accessible, resulting in truncated mRNAs. RelE causes the ribosome complexes to accumulate near the 5' end of genes as the process of ribosome rescue, translation, and cleavage by RelE repeats. RelE cleavage specific sub-codon level ribosome profiling data also represent reading frame in Escherichia coli and sequence specificity of RelE cleavage in vivo. We report another ribosome profiling study on a methyltransferase TrmD in E. coli. TrmD is known to methylate G37 (the residue at 3' side of anticodon) of some tRNAs and be responsible for codon-anticodon interaction. We constructed a TrmD depletion E. coli strain, whose deletion results in lethality of cells. Resulting depletion of m1G37 in the strain leads to growth arrest. Lack of m1G37 of some tRNAs whose codons start with C showed frequent frameshift when translating the gene message in vitro. By using ribosome profiling, we successfully observed significant difference on translation process when codons interact with anticodons of tRNAs lacking m1G37. The data reveal slow translation rate or pauses on the tRNAs when missing the appropriate methylation, which corresponds to the previous biochemical data in vitro.

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Mayer, Andreas [Verfasser], and Patrick [Akademischer Betreuer] Cramer. "Insights into the mRNA transcription cycle from genome-wide occupancy profiling / Andreas Mayer. Betreuer: Patrick Cramer." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1030210349/34.

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Huth, Antje [Verfasser], and Sabine [Akademischer Betreuer] Lutz-Bonengel. "Studien zum mRNA profiling an humanem postmortalem Gewebe / Antje Huth (geb. Koppelkamm). Betreuer: Sabine Lutz-Bonengel." Würzburg : Universitätsbibliothek der Universität Würzburg, 2012. http://d-nb.info/1029426805/34.

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Juusola, Jane. "MESSENGER RNA PROFILING: A PROTOTYPE METHOD FOR BODY FLUIDAND TISSUE IDENTIFICATION." Doctoral diss., University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3456.

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Conventional methods of body fluid identification use labor-intensive, technologically diverse techniques that are performed in a series, not parallel, manner and are costly in terms of time and sample. Furthermore, for some frequently encountered body fluids, such as saliva or vaginal secretions, no confirmatory technique exists. Terminally differentiated cells, such as blood lymphocytes or epithelial cells lining the oral cavity, have a unique pattern of gene expression, which is evinced by the presence and relative abundance of specific mRNA species. If the type and abundance of mRNAs can be determined in a stain or tissue sample recovered at the crime scene, it would be possible to definitively identify the tissue or body fluid in question. Advantages of an mRNA-based approach, compared to conventional biochemical analysis, include greater specificity, simultaneous and semi-automated analysis though a common assay format, improved timeliness, decreased sample consumption and compatibility with DNA extraction methodologies. In this report, we demonstrate that RNA is stable in biological stains and can be recovered in sufficient quantity and quality for analysis using reverse transcriptasepolymerase chain reaction assay (RT-PCR). We have identified sets of candidate tissuespecific genes for body fluids and tissues of forensic interest, namely blood, saliva, semen, vaginal secretions, menstrual blood, urine, skin, muscle, adipose, and brain. We also report the identification of a new housekeeping gene for use in mRNA based assays. Select body fluid-specific genes have been incorporated into multiplex PCR and real-time PCR assays. These assays allow for the positive identification of blood, saliva, semen,vaginal secretions, and/or menstrual blood in a stain. The final task of this work was the molecular characterization of mRNA degradation patterns in biological stains, which not only has fundamental importance in possibly revealing mRNA degradation pathways in dried biological stains, but may ultimately lead to better assay design strategies for mRNA markers for forensic use. An mRNA-based approach described in this report could allow the facile identification of the tissue components present in a body fluid stain and could conceivably supplant the battery of serological and biochemical tests currently employed in the forensic serology laboratory.<br>Ph.D.<br>Department of Chemistry<br>Burnett College of Biomedical Sciences<br>Biomolecular Sciences: Ph.D.

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Matilda, Rentoft. "The use of formalin fixed paraffin embedded tissue and global gene expression profiling for increased understanding of squamous cell carcinoma of the tongue." Doctoral thesis, Umeå universitet, Patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-54005.

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Head and neck cancer is the 6th most common malignancy worldwide, with tumours of the tongue being one of the most prevalent sites. Despite advances in surgery and radiotherapy, the five-year survival has not changed during the last decades and remains at approximately 50%. Identification of novel biomarkers for more personalized treatment is important for increasing survival in these patients. One of the most commonly used methods in the search for new biomarkers is microarray analysis. A substantial limitation with this technique is the requirement for fresh frozen samples from which high quality RNA can be extracted. This becomes particularly problematic when attempting to discover differences associated with individual sub-types or rare cancers. Recent developments, including the DASL microarray platform, have provided the possibility of analysing RNA of poorer quality from formalin fixed paraffin embedded (FFPE) samples. FFPE is the standard way of preserving tissue from patients and millions of samples are stored around the world. In this thesis we have evaluated the use of FFPE samples and global gene expression profiling for increasing basic knowledge in a subgroup of oral cancer patients with tumours of the tongue. As confirmation of microarray results using qPCR is of outmost importance for conclusive data evaluation, we first aimed at finding a housekeeping gene stably expressed across malignant and non-malignant FFPE oral tissue. TUBA6, which belongs to the tubulin family was detected as being the most stable out of eight possible genes and was thus used for qPCR normalization throughout the following studies. We have performed three separate microarray experiments. Initially only a focused DASL array covering 502 cancer related genes was available and we used it to analyze a smaller cohort of patients and controls (n=36). A similar cohort (n=29) was also analyzed for expression of 836 micoRNAs. In 2009 a whole genome DASL array was launched, covering over 20,000 genes, and all tongue tumour samples available between 1997 and 2010 (n=87) were analysed using this array. Similar to other research groups we observed very high replicate reproducibility using both DASL arrays. When using the microRNA array and the whole genome DASL array an effect of sample quality on the detected expression level of individual genes was noticed. While the expression of some genes severely decreased with a decrease in sample quality others were not changed. This will impair normalization, leading to a residual non-biological variation within the data. Based on our findings we have presented some recommendations for minimizing the effect of sample quality and maximizing the level of biologically relevant information obtained from these experiments, e.g. ensuring that samples in groups to be compared are of the same quality range. For the microRNA data we also introduced an additional normalization step to the standard normalizations. We could show that lists of differentially expressed genes generated when taking these precautions were enriched for genes involved in cancer related processes and contained for tongue carcinoma previously identified changes. A number of differentially expressed genes, novel for tongue carcinoma, were also confirmed in high quality fresh frozen samples, including BCL2A1 (apoptosis), CXCL10 (immune response), SLC2A6 (energy transport) and miR-424 (angiogenesis). In conclusion microarrays can be used to analyze FFPE samples but should be performed with care. Standard normalization methods will not remove the variation introduced by samples being of different quality, leading to spurious results. Taking a few precautions, however, led to the identification of differentially expressed genes relevant in tumour development and maintenance. The recommendations we make can facilitate design of future studies using FFPE samples. The genes we identified as being differentially expressed in tumour tissue now need to be further evaluated for their potential as biomarkers in tongue carcinoma.

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Islam, Md Aminul [Verfasser]. "Global mRNA and miRNA transcriptome profiling of peripheral blood mononuclear cells to investigate the host immunogenetic response to PRRSV vaccination in pigs / Md. Aminul Islam." Bonn : Universitäts- und Landesbibliothek Bonn, 2017. http://d-nb.info/1124590781/34.

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Book chapters on the topic "MRNA profiling"

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Willis, Dianna E., and Jeffery L. Twiss. "Profiling Axonal mRNA Transport." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-005-8_21.

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Jiao, Yuling, and José Luis Riechmann. "Genome-Wide Profiling of Uncapped mRNA." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-809-2_17.

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Roeder, Amy D., and Cordula Haas. "Body Fluid Identification Using mRNA Profiling." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3597-0_2.

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Kudla, Marek, and Fedor V. Karginov. "Measuring mRNA Translation by Polysome Profiling." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3591-8_11.

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Martins, Joao P. Sousa, and Marco Conti. "Profiling Maternal mRNA Translation During Oocyte Development." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8603-3_6.

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Ramachandran, Shyam, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray. "Microarray mRNA Expression Profiling to Study Cystic Fibrosis." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-120-8_12.

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Bustin, Stephen A., and Tania Nolan. "Real-Time Quantitative RT-PCR for mRNA Profiling." In Genomics. John Wiley & Sons, Ltd, 2010. http://dx.doi.org/10.1002/9780470711675.ch6.

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Kainkaryam, Raghunandan M., Angela Bruex, Peter J. Woolf, and John Schiefelbein. "Smart Pooling of mRNA Samples for Efficient Transcript Profiling." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-809-2_15.

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Jakubowska, Joanna, Agnieszka Maciejewska, and Ryszard Pawłowski. "mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3597-0_3.

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Rox, Jutta M., Jens Müller, and Bernd Pötzsch. "PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples." In DNA and RNA Profiling in Human Blood. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-553-4_18.

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Conference papers on the topic "MRNA profiling"

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Kabardaeva, K. V., O. N. Mustafaev, I. V. Deineko, A. V. Suhorukova, and I. V. Goldenkova-Pavlova. "Finding of regulatory codes in 5`-UTR of A. thaliana mRNAs by polysome profiling method." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.107.

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The polysome profiling method was used to separate mRNAs depending on their loading by ribosomes into polysomal and monosomal fractions. Pools separation of such mRNAs and analysis of transcripts (mRNAs) which are associated with each mRNA pool due to RNA sequencing allowed to get an idea of the translational efficiency of individual mRNAs. Moreover, subsequent in silico analysis make possible searching of regulatory contexts in the 5'-UTR of plant A. thaliana, which may be potentially important for efficient translation of mRNA.

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Wei, Qiao, Kuaile Zhao, Weiwei Yu, et al. "Abstract 3102: miRNA-mRNA regulatory networks by integrating miRNA and mRNA expression profiling of esophageal squamous cell carcinoma." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3102.

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Blackburn, MR, S. Zhang, L. Song, et al. "mRNA and microRNA Profiling in Mice Exhibiting Adenosine-Dependent Lung Disease." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1897.

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Yadav, Ravi Shanker, Surya Kant, Prashant Mani Tripathi, and Anuj Kumar Pandey. "Evaluation of mRNA expression profiling of NF-?B mediated signaling in COPD patients." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.2300.

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Haines, Zoe, Josh Cull, Samuel Baldwin, Guy Whitley, Angela Clerk, and Daniel Meijles. "BS23 mRNA expression profiling of dual specificity phosphatases (DUSPS) in the hypertensive heart." In British Cardiovascular Society Virtual Annual Conference, ‘Cardiology and the Environment’, 7–10 June 2021. BMJ Publishing Group Ltd and British Cardiovascular Society, 2021. http://dx.doi.org/10.1136/heartjnl-2021-bcs.221.

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Prabakaran, Indira, Paul J. Zhang, and Marina Guvakova. "Abstract B24: The diagnostic power of direct mRNA profiling in breast cancer core biopsy." In Abstracts: Fourth AACR International Conference on Frontiers in Basic Cancer Research; October 23-26, 2015; Philadelphia, PA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.fbcr15-b24.

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Mohammadnejad, Afsaneh, Shuxia Li, Hongmei Duan, et al. "Weighted gene co-expression network analysis of microarray mRNA expression profiling in response to electroacupuncture." In 2018 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2018. http://dx.doi.org/10.1109/bibm.2018.8621258.

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Giraldez, Maria D., Ryan M. Spengler, Alton Etheridge, et al. "Abstract IA23: Phospho-RNA-seq: A liquid biopsy approach for cell-free mRNA/lncRNA profiling." In Abstracts: AACR Special Conference on Advances in Liquid Biopsies; January 13-16, 2020; Miami, FL. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1557-3265.liqbiop20-ia23.

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Lapin, Morten, Kjersti Tjensvoll, Satu Oltedal, et al. "Abstract LB-252: Single-cell mRNA profiling reveal transcriptional heterogeneity among pancreatic circulating tumor cells." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-lb-252.

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Lee, Yi-Te, Na Sun, Ryan Y. Zhang, et al. "Abstract 5436: Purification and mRNA profiling of extracellular vesicles for early detection of hepatocellular carcinoma." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-5436.

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Reports on the topic "MRNA profiling"

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Albertson, Donna G. Cell Type-Specific mRNA Amplification and Expression Profiling from Breast Tumor Sections. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada382518.

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Albertson, Donna G. Cell Type-Specific mRNA Amplification and Expression Profiling from Breast Tumor Sections. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada405268.

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Albertson, Donna. Cell Type-Specific mRNA Amplification and Expression Profiling from Breast Tumor Sections. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada366918.

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