Academic literature on the topic 'MSC culture'

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Journal articles on the topic "MSC culture"

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Peters, R. E., M. Heikenwälder, and A. Knuth. "Expansion of umbilical cord blood mesenchymal stem cells." Journal of Clinical Oncology 27, no. 15_suppl (2009): 7103. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.7103.

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7103 Background: Umbilical cord blood (UCB) is known to harbor 2 major types of stem cells, the hematopoietic stem cells (HSC) & the non-hematopoietic or mesenchymal stem cells (MSC). Under appropriate conditions, MSCs can give rise to cells of bone, fat, hepatic lineages, etc. Based on this potential, MSC hold promise for clinical applications in regenerative medicine. Methods: Stroma-free liquid culture: UCB cryopreserved mononuclear cells (MNC) were cultured in the presence of early growth factors: Flt-3 & SCF (25ng/ml), MGDF (10ng/ml) & human serum (10%). MNC derived adherent M
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Prewitz, Marina, Friedrich Philipp Seib, Martin Bornhaeuser, and Carsten Werner. "Engineering Biomimetic Culture Systems: Impact On Human Bone Marrow-Derived Stem Cells." Blood 114, no. 22 (2009): 3628. http://dx.doi.org/10.1182/blood.v114.22.3628.3628.

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Abstract Abstract 3628 Poster Board III-564 The bone marrow (BM) harbours haematopoietic stem/progenitor cells (HSCs) in anatomically distinct sites (niches) where HSCs are subjected to regulatory cues such as cytokines, cell-cell contacts and extra-cellular matrix (ECM) all of which control stem cell fate. In particular mesenchymal stromal cells (MSCs) are an integral part of the bone marrow and are known to be key regulators of the HSC niche. We have previously shown that bio-artificial scaffolds can have a significant impact on the in vitro behaviour of MSCs. Here, we are therefore focussin
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Robinson, Simon N., Paul J. Simmons, Nathalie Brouard, et al. "Efficacy of ‘Off-the-Shelf’, Commercially-Available, Third-Party Mesenchymal Stem Cells (MSC) in Ex Vivo Cord Blood (CB) Co-Culture Expansion." Blood 110, no. 11 (2007): 4106. http://dx.doi.org/10.1182/blood.v110.11.4106.4106.

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Abstract INTRODUCTION: Our previous studies have shown that clinically-relevant levels of hematopoietic stem and progenitor cell (HSPC) expansion are possible by ex vivo co-culture of cord blood (CB) mononuclear cells (MNC) with third-party bone marrow (BM)-derived mesenchymal stem cells (MSC) and growth factors.1 A recently activated M. D. Anderson protocol requires that BM from a haplo-identical family member be used for the de novo generation of sufficient MSC for subsequent co-culture, a process requiring ∼3 weeks. Time constraints, uncertainties associated with the identification of a sui
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Bucher, Christian, Amiq Gazdhar, Lorin M. Benneker, Thomas Geiser, and Benjamin Gantenbein-Ritter. "Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System." Stem Cells International 2013 (2013): 1–9. http://dx.doi.org/10.1155/2013/326828.

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Intervertebral disc (IVD) cell therapy with unconditioned 2D expanded mesenchymal stem cells (MSC) is a promising concept yet challenging to realize. Differentiation of MSCs by nonviral gene delivery of growth and differentiation factor 5 (GDF5) by electroporation mediated gene transfer could be an excellent source for cell transplantation. Human MSCs were harvested from bone marrow aspirate and GDF5 gene transfer was achieved byin vitroelectroporation. Transfected cells were cultured as monolayers and as 3D cultures in 1.2% alginate bead culture. MSC expressed GDF5 efficiently for up to 21 da
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Tormin, Ariane, Jan Claas Brune, Stuart Walsh, Johan Richter, Xiaolong Fan, and Stefan Scheding. "Human Primary Mensenchymal Stromal Progenitor Cells Are Highly Enriched in Both, the CD271+/CD146+ and CD271+/CD146− Bone Marrow Population with the Latter Acquiring CD146 Expression upon Culture in-Vitro." Blood 112, no. 11 (2008): 2422. http://dx.doi.org/10.1182/blood.v112.11.2422.2422.

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Abstract Culture-derived mesenchymal stromal cells (MSC), which are attractive candidates for clinical cell therapy approaches, arise from primary MSC progenitor/stem cells in the bone marrow. Recently, several groups have reported markers (CD271, CD146, GD2, SSEA4, etc.) that allowed for an enrichment of CFU-F, i.e. primary MSC progenitors. However, the exact phenotype of the bona fide mesenchymal stem/progenitor cells has not yet not been sufficiently defined. We therefore aimed to investigate primary MSC in bone marrow subpopulations defined by the expression of CD271 and CD146, as both mar
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Giallongo, Cesarina, Daniele Tibullo, Nunziatina Laura Parrinello, et al. "Mesenchymal Stem Cells (MSC) from Patients with Multiple Myeloma Promote Myeloid Cells to Become Granulocytic-Myeloid-Derived Suppressor Cells (G-MDSC) with Immunosuppressive, Bone Resorption and Pro-Angiogenic Activity." Blood 128, no. 22 (2016): 4458. http://dx.doi.org/10.1182/blood.v128.22.4458.4458.

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Abstract Purpose A well-recognized feature of MM is the intimate relationship between plasma cells and bone marrow microenvironment, which is mainly composed of MSC, endothelial cells, immune cells and extracellular matrix. G-MDSC accumulate in the tumor microenvironment during tumor development promoting tumor growth and immunosuppression. Aim Analyzing MSC from MGUS, Smoldering myeloma (SMM) and MM patients in promoting G-MDSC generation. Methods Human peripheral blood mononucleated cells (PBMC) isolated from healthy subjects (HS) were cultured alone and with HS- (n=10), MGUS- (n=10), SMM- (
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Maijenburg, Marijke W., Marion Kleijer, Kim Vermeul, et al. "Primary Bone Marrow-Derived MSC Subsets Have Distinct Wnt-Signatures Compared to Conventionally Cultured MSC and Differ in Their Capacity to Support Hematopoiesis in Vitro,." Blood 118, no. 21 (2011): 3414. http://dx.doi.org/10.1182/blood.v118.21.3414.3414.

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Abstract Abstract 3414 Mesenchymal stromal cells (MSC) are of promising therapeutic use to suppress immunogenic responses following transplantation, and to support expansion of hematopoietic stem- and progenitors cells (HSPC) from small transplants derived for instance from cord blood. Culture-expanded MSC produce a wide variety and quantity of Wnt-proteins and the crucial role of Wnt-signaling in the hematopoietic stem cell niche is well established. However, studies addressing Wnt-signaling in MSC have (i) only focused on culture-expanded MSC and (ii) did not discriminate between phenotypica
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Burk, Janina, Anna Sassmann, Cornelia Kasper, Ariane Nimptsch, and Susanna Schubert. "Extracellular Matrix Synthesis and Remodeling by Mesenchymal Stromal Cells Is Context-Sensitive." International Journal of Molecular Sciences 23, no. 3 (2022): 1758. http://dx.doi.org/10.3390/ijms23031758.

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Matrix remodeling could be an important mode of action of multipotent mesenchymal stromal cells (MSC) in extracellular matrix (ECM) disease, but knowledge is limited in this respect. As MSC are well-known to adapt their behavior to their environment, we aimed to investigate if their mode of action would change in response to healthy versus pathologically altered ECM. Human MSC-derived ECM was produced under different culture conditions, including standard culture, culture on Matrigel-coated dishes, and stimulation with the pro-fibrotic transforming growth factor-β1 (TGFβ1). The MSC-ECM was dec
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Kaminska, Agnieszka, Aleksandra Wedzinska, Marta Kot, and Anna Sarnowska. "Effect of Long-Term 3D Spheroid Culture on WJ-MSC." Cells 10, no. 4 (2021): 719. http://dx.doi.org/10.3390/cells10040719.

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The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in
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Koh, Benson, Nadiah Sulaiman, Mh Busra Fauzi, et al. "A Three-Dimensional Xeno-Free Culture Condition for Wharton’s Jelly-Mesenchymal Stem Cells: The Pros and Cons." International Journal of Molecular Sciences 24, no. 4 (2023): 3745. http://dx.doi.org/10.3390/ijms24043745.

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Xeno-free three-dimensional cultures are gaining attention for mesenchymal stem cell (MSCs) expansion in clinical applications. We investigated the potential of xeno-free serum alternatives, human serum and human platelet lysate, to replace the current conventional use of foetal bovine serum for subsequent MSCs microcarrier cultures. In this study, Wharton’s Jelly MSCs were cultured in nine different media combinations to identify the best xeno-free culture media for MSCs culture. Cell proliferation and viability were identified, and the cultured MSCs were characterised in accordance with the
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Dissertations / Theses on the topic "MSC culture"

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Maillot, Charlotte. "Quantification and impact of microcarrier collisions during mesenchymal stem cell culture in bioreactors." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0314.

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Les thérapies cellulaires et tissulaires sont des thérapies innovantes à base de cellules du patient (on parle alors de thérapies autologues) ou de cellules d'un donneur compatible (on parle alors de thérapies allogéniques). Contrairement aux médicaments chimiques ou à base des protéines souvent recombinantes (anticorps monoclonaux par exemple), le fait d'utiliser des cellules en tant que produit permet d'envisager de nouvelles cibles thérapeutiques qui peuvent être difficiles d'accès, et aussi d'augmenter la spécificité en engageant une transition vers des médecines ciblées et personnalisées.
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Futrega, Katarzyna. "Device and application development for haematopoietic stem and progenitor cell (HSPC) and mesenchymal stromal cell (MSC) 3D spheroid cultures." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/92605/1/Katarzyna_Futrega_Thesis.pdf.

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With an overall aim to improve haematopoietic stem/progenitor cell (HSPC) and mesesnchymal stromal cells (MSC) 3D culture systems, this PhD Thesis addressed the following four interlinked AIMs: (1) The development of a high throughput microwell platform that enabled evaluation of MSC spheroid potential to expand HSPC in vitro; (2) Utilization of the high throughput microwell platform to manufacture HSPC/MSC spheroids to improve the efficacy of direct bone marrow transplantation; (3) The development of an improved microwell platform that retains spheroids within discrete microwells throughout c
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Corner, Helen. "An exploration into transfer of knowledge acquired from taught MSc Human Resource Management (HRM) programmes into workplace Human Resource (HR) Departments and wider dissemination across intra-organisational boundaries." Thesis, University of Derby, 2018. http://hdl.handle.net/10545/622720.

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The purpose of this thesis was to explore how knowledge gained during taught Masters in Human Resource Management (MSc HRM) programmes was transferred into working organisations, whether knowledge gained from academic study could be transferred if individuals were motivated to transfer and if organisations had a culture that was receptive to transfer. The term knowledge transfer was defined as sharing of information between one individual and another individual or group. This study looked at the perceived value of Human Resource (HR) knowledge within organisational contexts, with a focus on ho
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Schrön, Felix [Verfasser], Carsten [Akademischer Betreuer] Werner, Carsten [Gutachter] Werner, and Marcy [Gutachter] Zenobi-Wong. "Inkjet bioprinting and 3D culture of human MSC-laden binary starPEG-heparin hydrogels for cartilage tissue engineering / Felix Schrön ; Gutachter: Carsten Werner, Marcy Zenobi-Wong ; Betreuer: Carsten Werner." Dresden : Technische Universität Dresden, 2019. http://d-nb.info/1226944744/34.

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Monterosso, Melissa Eileen. "The Microwell-mesh platform: A multifaceted microtissue technology to link cell culture, animal models and patients." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/236375/1/Melissa_Monterosso_Thesis.pdf.

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Work towards effective translational medicine has revealed that current in vitro platforms are not sufficient to produce relevant clinical results. Advances in 3D technologies and preclinical models must be made to address the gaps lead to failure of initially promising therapies. The Microwell-mesh platform is a versatile device demonstrated to help bridge this gap. The device within the context of completed work was used to not only generate cancer xenograft models, crucial for successful personalized cancer therapeutics, but was also used to investigate the capacity of adult mesenchymal ste
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Chokder, Rafiul Abedin, and Tapia Paulina Vanessa Díaz. "The role of corporate culture in managing cultural diversity - A case study on a German multinational company." Thesis, Högskolan i Gävle, Avdelningen för ekonomi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-29275.

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Research Aim: Our aim is to understand how multinational companies integrate cultural diversity of employees in their corporate culture. To achieve this objective, we compare the employees’ perception with the company's view on the topic. Design/methodology/approach: A qualitative case study is conducted with three sets of questionnaires. Two sets of questionnaires were designed for the foreign and the local employees. The third set was created for the department of human resource management who represented the company’s view. Analysis is done by comparing the theories with empirical findings
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Zomer, Helena Debiazi. "Estabelecimento de cultura de células de pluripotência induzida a partir de células tronco derivadas do tecido adiposo de coelhos." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-18072014-103412/.

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As células de pluripotência induzida (iPS) foram reportadas pela primeira vez em 2006 por Takahashi e Yamanaka e desde então vem sendo extensivamente estudadas. Por meio da técnica, células somáticas adultas adquirem comportamento muito semelhante às células tronco embrionárias, reduzindo as questões éticas relacionadas ao uso destas em pesquisas. Entretanto, os mecanismos biológicos das células iPS ainda não estão completamente elucidados. Ainda são necessárias pesquisas mais aprofundadas para garantir a segurança e eficácia de sua possível utilização em futuras terapias. Os coelhos, como mod
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Tetenková, Pavla. "Diverzita a interkulturní aspekty fungování MNC." Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-261825.

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The thesis concentrates on culture, its levels and characteristics, including business culture. The first part focuses on cultural diversity and management of diversity in business culture, with emphasis on the related incorrect tendencies, caused by various culturally conditioned biases. The following part considers different aspects of intercultural communication, its possible barriers and the question of intercultural training. The practical part analyses an existing multinational corporation, particularly with regards to its corporate values, practices, corporate diversity and intercultura
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Inacio, Fatima Pacheco de Santana. "A política de formação de professores em Goiás no contexto dos acordos MEC-USAID (1961-1983)." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/251183.

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Orientador: Ediogenes Aragão Santos<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Educação<br>Made available in DSpace on 2018-08-19T01:10:42Z (GMT). No. of bitstreams: 1 Inacio_FatimaPachecodeSantana_D.pdf: 205029521 bytes, checksum: 5e0312c25a9d06d86af947a1528d72b5 (MD5) Previous issue date: 2011<br>Resumo: Este texto analisa a formação de professores, realizada no âmbito das políticas públicas decorrentes dos acordos MEC/USAID/UNESCO, no pós Segunda Guerra Mundial, que resultaram na criação dos Centros de Formação de Professores Primários, entre 1961 e 1983, no mun
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Pina, Fabiana [UNESP]. "O acordo MEC-USAID: ações e reações (1966 – 1968)." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/93369.

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Made available in DSpace on 2014-06-11T19:26:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-25Bitstream added on 2014-06-13T20:47:34Z : No. of bitstreams: 1 pina_f_me_assis.pdf: 558950 bytes, checksum: 60aa1e70aec72204edda905a19c4a6a6 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>O presente trabalho procura analisar o Acordo MEC-USAID, buscando destacar sua importância para a estrutura universitária brasileira, as modificações ainda presentes que partiram deste Acordo e o contexto histórico no qual ele foi efetivado. Procuramos desenvolver uma
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Books on the topic "MSC culture"

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Martins, Maria do Rosa rio Rodrigues., ред. Moc ʹambique, aspectos da cultura material. Universidade de Coimbra, Instituto de Antropologia, 1986.

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Michael, Fraase, ed. Guide to the Macintosh underground: Mac culture from the inside. Hayden Books, 1993.

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author, Simion Nina, ed. Mic dicționar de artă sacră și de cultură veche românească. Basilica, 2017.

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Bancks, Tristan. Mac Slater hunts the cool. Simon & Schuster Books for Young Readers, 2010.

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Bancks, Tristan. Mac Slater vs. the city. Simon & Schuster Books for Young Readers, 2011.

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Bancks, Tristan. Mac Slater hunts the cool. Simon & Schuster Books for Young Readers, 2010.

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Montandon, Mac. Jetpack dreams: One man's up and down (but mostly down) search for the greatest invention that never was. Da Capo Press, 2008.

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Sanborn, Michelle. Teaching about world cultures: Focus on developing regions. Center for Teaching International Relations, University of Denver, 1986.

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Sociedade dos Amigos Ouvintes da Rádio MEC. Programas educativos e culturais escritos para a Rádio MEC de 1947 a 2009. Sociedade dos Amigos Ouvintes da Rádio MEC, 2009.

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Austria. Das Ausfuhrverbotsgesetz für Kulturgut (AusfVKG): Zugleich 2. Ergänzungsband zu Die Rechtsvorschriften für den Denkmalschutz (MSA 49). Manz, 1987.

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Book chapters on the topic "MSC culture"

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Vischi, Alessandra. "Employability and Transitions towards Work: MSc Degree Programme in Educational Planning and Human Resource Development, Catholic University of the Sacred Heart of Brescia." In Employability & Competences. Firenze University Press, 2018. http://dx.doi.org/10.36253/978-88-6453-672-9.50.

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The acceleration of changes underlines the need to enhance our efforts to adapt education to the dynamics of the current economic situation and the issue of employment. In the framework of the circular economy, pedagogy, which is based on the educability of individuals, takes into consideration forms of educational planning to identify a long-lasting balance between economic prosperity, social wellness, and environmental development. The challenge of the future is the possibility of increasing youth employment; this calls for pedagogical expertise and organizational planning to ensure that eve
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Dean, Joan FitzPatrick, and Radvan Markus. "The Internationalist Dramaturgy of Hilton Edwards and Micheál mac Liammóir." In Cultural Convergence. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57562-5_2.

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Abstract In part because the Gate explored an experimental dramaturgy, its artistic directors Micheál mac Liammóir and Hilton Edwards often wrote, spoke and advocated for a drama that could move beyond realism. The analysis of Hilton Edwards’s dramatic commentary reaches from his early articles on dramaturgy right up to his encounter with the Berliner Ensemble in 1956 that influenced Edwards’s most elaborate statement on drama, The Mantle of Harlequin (1958). An important part of Edwards’s vision was his cosmopolitanism, his refusal to view drama within a restricted national framework. Nationa
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Clare, David, and Nicola Morris. "The Transnational Roots of Key Figures from the Early Years of the Gate Theatre, Dublin." In Cultural Convergence. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57562-5_4.

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Abstract In Gate Theatre studies, the venue’s original artistic directors, Hilton Edwards and Micheál mac Liammóir, are commonly described as ‘Englishmen’. This chapter breaks new ground by exploring the Irish roots of Edwards and mac Liammóir, and the rumours that mac Liammóir had Spanish and Jewish ancestry. ‘The Boys’ were not the only figures associated with the early Gate to have transnational backgrounds. Coralie Carmichael, the theatre’s biggest female star in its early years, was of mixed Moroccan and Scottish ancestry, and Nancy Beckh, who worked as an actor, costume designer and mill
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Kallunki, Jarmo, Jaakko Kauko, and Oren Pizmony-Levy. "Finland’s Ministry of Education and Culture in the Light of Its Working Groups." In Finland’s Famous Education System. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-8241-5_3.

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AbstractThe Ministry of Education and Culture (MEC) has traditionally been considered as the most central actor and the powerhouse in the education policy field in Finland. While the position of the MEC in the Finnish education policy system seems stable, there have been several organisational changes within the MEC over the past three decades. One of these is the disintegration of the committee system and its replacement by the working groups system, a trend that is part of a more general change from governing to governance since the 1990s. In this chapter we analyse data containing the MEC’s
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Sisson, Elaine. "Kismet: Hollywood, Orientalism and the Design Language of Padraic Colum’s Mogu of the Desert." In Cultural Convergence. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57562-5_7.

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Abstract The lure of the exotic ‘other’ was implicit from the early years of the Gate’s repertoire. In 1931 the Gate produced Padraic Colum’s Mogu of the Desert, designed by Micheál mac Liammóir and featuring a young Orson Welles. Exploring Mogu uncovers a broader engagement with ‘exotic’ or oriental narratives at the Gate generally. The history and subject matter of Mogu contextualizes mac Liammóir’s fascination with oriental and Middle-Eastern culture within contemporary film. Archival photos illustrate how production stills copied the iconographic styling of film publicity using ‘film-star’
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Todesco, Gian Marco. "M.C. Escher e il piano iperbolico." In Matematica e cultura 2010. Springer Milan, 2010. http://dx.doi.org/10.1007/978-88-470-1594-4_10.

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Trabelsi, Khaled, Semy Majoul, Fatma Charfi, and Héla Kallel. "Measles Virus Production in MRC-5 Cells Grown on Microcarriers in a Stirred Bioreactor." In Cells and Culture. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_133.

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Pilný, Ondřej. "The Brothers Čapek at the Gate: R.U.R. and The Insect Play." In Cultural Convergence. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57562-5_6.

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Abstract The chapter examines two important but hitherto largely neglected productions of famous European dramas at Dublin’s Gate Theatre, R.U.R. (1929, revived 1931) and The Insect Play (1943) by the brothers Čapek, which it attempts to reconstruct insofar as the available documentary evidence allows. In the process, it discusses the complicated textual history of the English versions produced by the Gate and compares their staging by Hilton Edwards and Micheál mac Liammóir with the celebrated original productions in Czechoslovakia. Finally, the reception of the respective productions is juxt
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McIvor, Charlotte. "Ghosting Bridgie Cleary: Tom Mac Intyre and Staging This Woman’s Death." In Crossroads: Performance Studies and Irish Culture. Palgrave Macmillan UK, 2009. http://dx.doi.org/10.1057/9780230244788_14.

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Ivory, Yvonne. "Prussian Discipline and Lesbian Vulnerability: Christa Winsloe’s Children in Uniform at the Gate." In Cultural Convergence. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-57562-5_8.

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Abstract This chapter examines the Dublin production and critical reception of Christa Winsloe’s Children in Uniform, which ran to full houses at the Gate for three weeks in April 1934. The play, which deals with the love between a Prussian schoolgirl and her female teacher, had premiered in Leipzig (1930), run successfully in Berlin (1931), and been adapted for the screen as Mädchen in Uniform (1931) before it was translated into English for a successful London run in 1932-1933. Edwards and mac Liammóir probably saw the original German play in Berlin in 1931. Using the prompt copy, lighting p
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Conference papers on the topic "MSC culture"

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Shukla, Pavan K., Roderick E. Fuentes, Andrew Nordquist, Bruce J. Wiersma, and Ingrid Pederson. "Effectiveness of Vapor Corrosion Inhibitors under Bacterial Activity for Aboveground Storage Tank Application." In CONFERENCE 2024. AMPP, 2024. https://doi.org/10.5006/c2024-20789.

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Abstract Bacterial activity is known to influence corrosion and can result in increasing soil-side corrosion of the aboveground storage tanks (ASTs) floor plates. Association for Materials Protection and Performance (AMPP) defines this phenomenon as Microbiologically Influenced Corrosion (MIC). It has been hypothesized that vapor corrosion inhibitors (VCIs) can not only function in presence of bacterial activity but can also function as biocides. Experiments were conducted to study the effect of bacterial activity on corrosion in the sand pad conditions and effect of VCIs in mitigating MIC. Va
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Brutinel, Evan, Ben Wilson, and Kristi Gangelhoff. "Evaluation of a Self-contained, Anaerobic Environment-generating Culture Device for Detection of Sulfate-reducing Bacteria." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13174.

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Absrract Certain classes of microorganisms (e.g., sulfate reducing bacteria or SRB) have been linked to an increased risk of microbiologically influenced corrosion (MIC) and reservoir souring. The ability to quickly and accurately detect and enumerate MIC-associated microbes is an essential part of an informed and efficacious mitigation strategy. Despite advances in molecular microbiology methods (MMMs) and adenosine triphosphate (ATP) detection technologies, culture-based detection remains a key aspect of industrial and oil field microbiology. Detection and enumeration of MIC-causing microbes
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Dockens, Kim, Shawna Johnston, Marc Demeter, and Stan Leong. "Comparison of Planktonic and Sessile Bacteria Counts Using ATP and DNA Based Methods." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09414.

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Abstract Microbiologically influenced corrosion (MIC) is a term used to describe corrosive damage to metals caused by microbes. Corrosion damage is costly to operations, the environment and life. As such, monitoring for, and diagnosing MIC as part of a complete corrosion mitigation strategy is of paramount importance. Traditional MIC diagnostic techniques employ culture-based methods aimed at enumerating microbes presumed to be associated with MIC. Culture-based diagnostics are time consuming and may severely under estimate populations. Moreover, microbes mediating corrosion typically exhibit
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Pagnussat, Mariana, Mónica Zapponi, Verónica Rodríguez Bauzada, et al. "Evaluation of Polymeric Coatings for MIC Problems During Oil and Gas Transportation." In LatinCORR 2023. AMPP, 2023. https://doi.org/10.5006/lac23-20428.

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Extended Abstract During oil and gas operations, pipelines are subjected to corrosion deterioration mechanisms, one of which is microbiologically influenced corrosion (MIC). The aim of this work was to analyze the behavior of different polymeric coatings in a continuous culture of sulfate-reducing bacteria (SRB) after 3 and 6 months of exposure. L80 API carbon steel samples with different coatings were placed in the bioreactor. At the end of the exposure periods, the samples were scraped with a sterile scalpel and cultured in Postgate B liquid medium to count biofilm-forming bacteria. Control
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Kotu, Susmitha Purnima, Celal Erbay, Nebras Sobahi, Arum Han, Sam Mannan, and Arul Jayaraman. "Integration of Electrochemical Impedance Spectroscopy and Microfluidics for Investigating Microbially Influenced Corrosion Using Co-culture Biofilms." In CORROSION 2016. NACE International, 2016. https://doi.org/10.5006/c2016-07793.

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Abstract Microbially influenced corrosion (MIC) is a major problem in various sectors including chemical process plants, on-shore and off-shore oil and gas, pipelines, marine and aviation industries resulting in annual losses just in the United States of several billions of dollars. MIC often develops as a result of biofilm formation by multiple microbial species that form well-defined and organized structures. Development of effective mitigation strategies for MIC requires a fundamental understanding of how biofilms are formed. The aim of this study was to investigate the factors underlying f
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Kilbane, John J., Bill Bogan, and Brigid Lamb. "Quantifying the Contribution of Various Bacterial Groups to Microbiologically Influenced Corrosion." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05491.

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Abstract It is well known that various types of bacteria are relevant to microbiologically influenced corrosion (MIC); however, it is not clear what the quantitative contribution of various types of bacterial groups may be. Mixed cultures and experimental conditions representative of actual field conditions where MIC occurs in gas industry operations were used in conjunction with various chemicals that can selectively inhibit the growth and/or activity of various microbial groups. In this way the contribution of the various types of microbial groups to MIC was determined. Sulfate reducing bact
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Sublette, Kerry, Richard Eckert, Dora Ogles, Brett Baldwin, and Katherine Clark. "Molecular Analysis of Aramid Polymer Film Impressions of Corrosion Coupon Biofilms." In CORROSION 2014. NACE International, 2014. https://doi.org/10.5006/c2014-4477.

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Abstract Microbiologically influenced corrosion (MIC) is a serious problem that impacts nearly all industries and exacts a severe toll in terms of operating costs, loss of production, deterioration of capital equipment and the consequences of corrosion related failures. While a proactive step, monitoring for MIC-associated microorganisms is often hindered by the fact that sampling methods may not capture key members of the microbial community involved in MIC (sampling biases) and current reliance on conventional culture-dependent methods may underestimate and oversimplify the problem (cultivat
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Olabisi, Olagoke, Saleh Al-Sulaiman, Amer Jarragh, and Adel Al-Mutairi. "Ribosomal RNA Characterization of Bacteria: Linkage with Field Data Based on Culture Media." In CORROSION 2015. NACE International, 2015. https://doi.org/10.5006/c2015-05591.

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Abstract One of the major challenges that face our national oil company is microbiologically influenced corrosion (MIC). In spite of biocide treatment in the facilities, the serial dilution results continue to confirm a high proliferation of sessile and planktonic bacteria in all the water handling systems. Planktonic and sessile samples from different gathering centers are now being analyzed for their molecular identities based on 16S rRNA characterization employing a molecular microbiological method (MMM) of quantitative polymerase chain reaction (qPCR) for enumeration of specific microorgan
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Borenstein, S. W., and P. B. Lindsay. "Planning and Conducting Microbiologically Influenced Corrosion Failure Analyses." In CORROSION 1987. NACE International, 1987. https://doi.org/10.5006/c1987-87381.

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Abstract Microbiologically influenced corrosion (MIC) can be misdiagnosed as attack due to conventional chloride crevice/pitting corrosion unless specialized techniques are used during the failure analysis. These techniques include in-situ bacterial sampling of residual water, bacterial analysis of corrosion products using analytical chemistry, culture growth, and scanning electron microscopy, as well as nondestructive examination (NDE) using ultrasonics and radio-graphic techniques. Metallographic examination can reveal MIC characteristics such as dendritic corrosion attack in weld metal. If
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Demeter, Marc, Shawna Johnston, Kim Dockens, and Raymond J. Turner. "Molecular MIC Diagnoses from ATP Field Test: Streamlined Workflow from Field to 16S rRNA Gene Metagenomics Results." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09420.

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Abstract Microbiologically influenced corrosion (MIC) describes the negative impacts of microbial growth within various industries. While culture based growth tests are the traditional means of assessing MIC, they are not accurate. New culture-independent assays have been developed; one of which is the adenosine triphosphate (ATP) assay. This field assay allows for indirect enumeration of microorganisms; however, it does not divulge which microorganisms are present. Molecular methods such as 16S metagenomics (16S rRNA gene sequencing and data interpretation) are often used for characterizing t
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Reports on the topic "MSC culture"

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Wick, Charles H., and Patrick E. McCubbin. Removing Complex Growth Media from MS2 Bacteriophage Cultures. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada368537.

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Sawangmake, Chenphop. Development of genetic manipulation approach for in vitro production of islet-like cell cluster (ILCCs) or insulin-producing cells (IPCs) from canine bone marrow-derived mesenchymal stem cells (cBM-MSCs). Chulalongkorn University, 2018. https://doi.org/10.58837/chula.res.2018.88.

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In this study, trend of stem cell-based treatment for diabetes type 1 in veterinary practice has been preliminarily investigated. Production of pancreatic lineages by canine bone marrow-derived mesenchymal stem cells (cBM-MSCs) using genetic manipulation approach has been studied. Transfection efficiency of second-generation lentiviral vector on cBM-MSCs employing “pLenti CMV GFP Puro (658-5)” (Addgene plasmid #17448) was investigated and the results suggested the susceptibility of the cell to such transfection. Further study was performed to evaluate the efficiency of PDX1 transfection on cBM
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Jact. L52122 Mitigation of MIC using CP Under Disbonded Coating. Pipeline Research Council International, Inc. (PRCI), 2008. http://dx.doi.org/10.55274/r0011099.

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This report summarizes lab work carried out in a test cell in which line pipe steel was exposed under anaerobic conditions to a freshly prepared synthetic corrosion product consisting of iron sulfide and iron carbonate under a permeable �coating� barrier. Weight loss for the steel specimen was sharply reduced by application of CP with rates of corrosion for short term experiments dropping to less than 0.05 mm/year at potentials more negative than �950 mVCSE at the pipe surface. This value is in good agreement with past literature. Addition of a mixed microbial culture including SRB to the expe
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Estrada Villaseñor, Cecilia, Adam Dubin Edelstein, Jose Manuel Aparicio Malo, and Raquel Verdasco Martínez. Data Culture in Human Trafficking II: Technical Research Report. Universidad Pontificia Comillas, 2024. http://dx.doi.org/10.14422/iuem.20241031.

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Human trafficking is recognized worldwide as a form of modern-day slavery that continues to devastate lives and communities. This criminal phenomenon not only constitutes a serious violation of human rights, but also represents one of the most lucrative illicit businesses globally, compared only to drug and arms trafficking. In this context, the collection and analysis of accurate and reliable data is essential to understand the magnitude and characteristics of human trafficking at our borders and to develop effective public policies. This report, developed within the framework of the "Data Cu
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Pound. L52104 Differentiation of Corrosion Mechanisms by Morphological Feature Characterization. Pipeline Research Council International, Inc. (PRCI), 2004. http://dx.doi.org/10.55274/r0011097.

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Corrosion of liquid and gas pipelines can occur by various mechanisms. The ability to differentiate between mechanisms is crucial if corrosion control measures are to be effective. The objective of this work was to determine whether corrosion of pipeline steels results in characteristic morphological features that are diagnostic for specific corrosion mechanisms, particularly with regard to microbiologically influenced corrosion (MIC). Coupons of 1018 carbon steel were exposed for two weeks in 5 wt% NaCl under abiotic and biotic conditions in different environments (N2, N2-CO2, and N2-H2S). Pi
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Estrada Villaseñor, Cecilia, Adam Dubin Edelstein, Jose Manuel Aparicio Malo, and Raquel Verdasco Martínez. Cultura de Datos en Trata de Seres Humanos II: Informe Técnico de Investigación. Universidad Pontificia Comillas, 2024. http://dx.doi.org/10.14422/iuem.20240923.

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La trata de personas es reconocida mundialmente como una forma de esclavitud mo¬derna que continúa devastando vidas y comunidades. Este fenómeno delictivo no solo constituye una grave violación de los derechos humanos, sino que también representa uno de los negocios ilícitos más lucrativos a nivel global, comparado solo con el tráfico de drogas y armas. En este contexto, la recopilación y análisis de datos precisos y fiables es fundamental para comprender la magnitud y las características de la trata de personas en nuestras fronteras y para desarrollar políticas públicas efectivas. Este inform
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Romero Ramírez, Álvaro Fernando, and Felipe Eugenio Mora Parra. Plan estratégico y prospectivo del municipio de la Plata Huila como eje regional de desarrollo de turismo sostenible 2020 – 2030. Universidad Nacional Abierta y a Distancia, 2021. http://dx.doi.org/10.22490/ecacen.5439.

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El turismo en Colombia, es un sector importante en la economía; el departamento del Huila posee un potencial alto de desarrollo en este sector; este trabajo se realizó en el municipio de La Plata, en un ámbito de desarrollo turístico sostenible; entendiendo esto como el equilibrio entre el sector privado, publico, académico y la comunidad. El objetivo del presente trabajo fue estructurar el plan estratégico y prospectivo del municipio de La Plata Huila para convertirlo como eje regional de desarrollo en el turismo sostenible 2020 – 2030. Para esto se utilizaron los métodos de prospectiva estra
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Jorgensen, Frieda, John Rodgers, Daisy Duncan, Joanna Lawes, Charles Byrne, and Craig Swift. Levels and trends of antimicrobial resistance in Campylobacter spp. from chicken in the UK. Food Standards Agency, 2022. http://dx.doi.org/10.46756/sci.fsa.dud728.

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Campylobacter spp. are the most common bacterial cause of foodborne illness in the UK, with chicken considered to be the most important vehicle of transmission for this organism. It is estimated there are 500,000 cases of campylobacteriosis in the UK annually, with Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) accounting for approximately 91% and 8 % of infections, respectively. Although severe infection in humans is uncommon, treatment is seldom needed for human infection but usually involves the administration of a macrolide (e.g., azithromycin) or a fluoroquinolone (e.g.
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Day, Kelsey. Nursing students' self-efficacy in caring for gender-diverse patients. University of Missouri - Columbia, 2024. https://doi.org/10.32469/10355/106482.

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Background Gender-diverse patients (GDP) face barriers in healthcare, including discrimination and limited access to preventive care. Nursing curricula often lack GDP health content, leaving students underprepared to provide equitable care. This QI project aimed to improve RN-BSN students' self-efficacy and comfort in caring for GDP by integrating an educational module into their coursework. Methods A literature review revealed the need for increased education on GDP healthcare needs. An evidence-based educational module including a recorded presentation and an interactive case study was devel
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‘Understanding developmental cognitive science from different cultural perspectives’ – In Conversation with Tochukwu Nweze. ACAMH, 2020. http://dx.doi.org/10.13056/acamh.13666.

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Tochukwu Nweze, lecturer in the Department of Psychology, University of Nigeria, Nsukka and, PhD student in MRC Cognition and Brain Sciences Unit, University of Cambridge talks about his recent paper on parentally deprived Nigerian children having enhanced working memory ability, how important is it to study cultural differences in cognitive adaption during and following periods of adversity, and how can mental health professionals translate this understanding of difference into their work.
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